efna5b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	efna5b
Entrez Gene ID	30223
Full Name	ephrin-A5b
Synonyms	EPLG7,L4,ZfEphL4,al1,efna5,lerk7,tkral1
General protein information	Preferred Names ephrin-A5b Names ephrin-A5b AL-1 EPH-related receptor tyrosine kinase ligand 7 LERK-7 tyrosine kinase ligand AL-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131026.2	NP_571101.1	ephrin-A5b precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC608458	XP_850582.2
Pan troglodytes (chimpanzee)	EFNA5	XP_526972.2
Rattus norvegicus (Norway rat)	Efna5	NP_446355.1
	EFNA5	XP_001099753.1
Bos taurus (cattle)	EFNA5	NP_001069900.1
Danio rerio (zebrafish)	efna5b	NP_571101.1
Homo sapiens (human)	EFNA5	NP_001953.1
Mus musculus (house mouse)	Efna5	NP_997537.1
Gallus gallus (chicken)	EFNA5	NP_990515.1
Xenopus tropicalis (tropical clawed frog)	efna5	NP_001016422.1

Gene Ontology
Bibliography

Related articles in PubMed

Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer.
Zhang J, Jiang Z, Liu X, Meng A
Development (Cambridge, England)143(14)2603-15(2016 07)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Protein tyrosine phosphatase receptor type O (Ptpro) regulates cerebellar formation during zebrafish development through modulating Fgf signaling.
Liao WH, Cheng CH, Hung KS, Chiu WT, Chen GD, Hwang PP, Hwang SP, Kuan YS, Huang CJ
Cellular and molecular life sciences : CMLS70(13)2367-81(2013 Jul)

Retinotopic order in the absence of axon competition.
Gosse NJ, Nevin LM, Baier H
Nature452(7189)892-5(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following efna5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efna5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03361	NM_131026.2 Latest version!	Danio rerio ephrin-A5b (efna5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

efna5b ( NM_131026.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571101.1 Danio rerio ephrin-A5b (efna5b), mRNA.

Price & Availability↑

CloneID	ODa03361
Clone ID Related Accession (Same CDS sequence)	NM_131026.2
Accession Version	NM_131026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Danio rerio(zebrafish)
Product	ephrin-A5b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y09669.1. On May 25, 2007 this sequence version replaced NM_131026.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y09669.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCAGG CGGAGATGAT CGTGTTCGTG GGTGTGATTC TGTGGATGTG TGTATTTAGT 
CAGGAGCCCA GCTCAAAGGT GATGGCTGAT CGATATGCTG TCTTCTGGAA CCGAACCAAC 
CCCAGGTTTC AGCGTGGAGA TTACCACATC GACGTGTGTA TAAACGACTA TCTGGACGTG 
TACTGTCCGC ATTACGAGGA CTCGGTGCCG GAGGAGCGTA CGGAGAGATA TGTTCTCTAC 
ATGGTGAATT ATGACGGATA CAGCACCTGC GACCACACGG CCAAAGGATT CAAGCGCTGG 
GAGTGCAATC GGCCTCATTC CCCCAACGGG CCGCTCAAGT TCTCGGAGAA ATTTCAGCTT 
TTTACACCCT TTTCTCTGGG CTTCGAGTTC AGACCCGGAC GGGAGTATTA CTACATCTCC 
TCCATGATCA CAGAAACCGG CAGAAGGTCA TGTCTCAAGC TGAAGGTCTT CGTGAGGCCG 
CCGAATGGCT GTGAGAAAAC CATAGGCGTT CATGACCGCG TTTTTGTCGA TGACAAAGTA 
GACAATGCAT TAGAACCAAG AGACGACACG AGTCACGAGG CTGAACCATC CCGGAGCGAC 
GTGTCGACTT CAGGCCTGCG GCACCAGACG TCGCGCCCCC TGCTGGCGCT CCTGCTGCTC 
TGCATCTCGC TCTATCTGCT CTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571101.1
CDS	200..886
Translation	MLQAEMIVFVGVILWMCVFSQEPSSKVMADRYAVFWNRTNPRFQRGDYHIDVCINDYLDVYCPHYEDSVPEERTERYVLYMVNYDGYSTCDHTAKGFKRWECNRPHSPNGPLKFSEKFQLFTPFSLGFEFRPGREYYYISSMITETGRRSCLKLKVFVRPPNGCEKTIGVHDRVFVDDKVDNALEPRDDTSHEAEPSRSDVSTSGLRHQTSRPLLALLLLCISLYLLL

Target ORF information:

RefSeq Version	NM_131026.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ephrin-A5b (efna5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131026.2

ORF Insert Sequence:

ATGCTGCAGG CGGAGATGAT CGTGTTCGTG GGTGTGATTC TGTGGATGTG TGTATTTAGT 
CAGGAGCCCA GCTCAAAGGT GATGGCTGAT CGATATGCTG TCTTCTGGAA CCGAACCAAC 
CCCAGGTTTC AGCGTGGAGA TTACCACATC GACGTGTGTA TAAACGACTA TCTGGACGTG 
TACTGTCCGC ATTACGAGGA CTCGGTGCCG GAGGAGCGTA CGGAGAGATA TGTTCTCTAC 
ATGGTGAATT ATGACGGATA CAGCACCTGC GACCACACGG CCAAAGGATT CAAGCGCTGG 
GAGTGCAATC GGCCTCATTC CCCCAACGGG CCGCTCAAGT TCTCGGAGAA ATTTCAGCTT 
TTTACACCCT TTTCTCTGGG CTTCGAGTTC AGACCCGGAC GGGAGTATTA CTACATCTCC 
TCCATGATCA CAGAAACCGG CAGAAGGTCA TGTCTCAAGC TGAAGGTCTT CGTGAGGCCG 
CCGAATGGCT GTGAGAAAAC CATAGGCGTT CATGACCGCG TTTTTGTCGA TGACAAAGTA 
GACAATGCAT TAGAACCAAG AGACGACACG AGTCACGAGG CTGAACCATC CCGGAGCGAC 
GTGTCGACTT CAGGCCTGCG GCACCAGACG TCGCGCCCCC TGCTGGCGCT CCTGCTGCTC 
TGCATCTCGC TCTATCTGCT CTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer.
Development (Cambridge, England)143(14)2603-15(2016 07)
Zhang J,Jiang Z,Liu X,Meng A

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Protein tyrosine phosphatase receptor type O (Ptpro) regulates cerebellar formation during zebrafish development through modulating Fgf signaling.
Cellular and molecular life sciences : CMLS70(13)2367-81(2013 Jul)
Liao WH,Cheng CH,Hung KS,Chiu WT,Chen GD,Hwang PP,Hwang SP,Kuan YS,Huang CJ

Retinotopic order in the absence of axon competition.
Nature452(7189)892-5(2008 Apr)
Gosse NJ,Nevin LM,Baier H