utp25 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	utp25
Entrez Gene ID	286787
Full Name	digestive organ expansion factor homolog
Synonyms	chunp6870,def,dj434o14.5,dj434o14.5l,fc51g08,fi05f05,wu:fc51g08,wu:fi05f05
General protein information	Preferred Names digestive organ expansion factor homolog Names digestive organ expansion factor digestive-organ expansion factor lily liver lly
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173273.1	NP_775380.1	digestive organ expansion factor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DIEXF	XP_547394.3
Kluyveromyces lactis	KLLA0E08229g	XP_454321.1
Neurospora crassa	NCU01022	XP_961634.1
Gallus gallus (chicken)	DIEXF	NP_001026222.1
Danio rerio (zebrafish)	diexf	NP_775380.1
Drosophila melanogaster (fruit fly)	CG3735	NP_611851.1
Saccharomyces cerevisiae (baker's yeast)	UTP25	NP_012175.1
Xenopus tropicalis (tropical clawed frog)	diexf	NP_001008035.1
Pan troglodytes (chimpanzee)	DIEXF	XP_525053.2
Mus musculus (house mouse)	Diexf	NP_663390.2
Rattus norvegicus (Norway rat)	Diexf	NP_001014008.1
Magnaporthe oryzae (rice blast fungus)	MGG_10652	XP_003710081.1
	DIEXF	XP_001109922.1
Oryza sativa (rice)	Os05g0295100	NP_001055109.1
Homo sapiens (human)	DIEXF	NP_055203.4
Bos taurus (cattle)	DIEXF	NP_001098822.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011170	XP_309479.4
Eremothecium gossypii	AGOS_ADL209C	NP_983887.1
Schizosaccharomyces pombe (fission yeast)	SPCC1827.01c	NP_588547.1
Arabidopsis thaliana (thale cress)	NOF1	NP_564032.1

Gene Ontology
Bibliography

Related articles in PubMed

The pre-rRNA processing factor DEF is rate limiting for the pathogenesis of MYCN-driven neuroblastoma.
Tao T, Sondalle SB, Shi H, Zhu S, Perez-Atayde AR, Peng J, Baserga SJ, Look AT
Oncogene36(27)3852-3867(2017 Jul)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

An equation to estimate the difference between theoretically predicted and SDS PAGE-displayed molecular weights for an acidic peptide.
Guan Y, Zhu Q, Huang D, Zhao S, Jan Lo L, Peng J
Scientific reports513370(2015 Aug)

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
Tao T, Shi H, Guan Y, Huang D, Chen Y, Lane DP, Chen J, Peng J
Cell research23(5)620-34(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fibrotic scar formation in def+/- is blocked by the over-expression of Def.
Title: Haploinsufficiency of Def activates p53-dependent TGF? signalling and causes scar formation after partial hepatectomy.

Def can mediate the degradation of p53 protein and that this process is independent of the proteasome pathway, but dependent on the activity of Calpain3, a cysteine protease.
Title: Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.

Def functions in a cell autonomous manner during early liver development.
Title: Def functions as a cell autonomous factor in organogenesis of digestive organs in zebrafish.

def gene encodes a novel pan-endoderm-specific factor.p53 is tightly regulated by factor Def, especially during organogenesis
Title: Loss of function of def selectively up-regulates Delta113p53 expression to arrest expansion growth of digestive organs in zebrafish.

The following utp25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the utp25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37464	NM_173273.1 Latest version!	Danio rerio UTP25 small subunit processor component (utp25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

utp25 ( NM_173273.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775380.1 Danio rerio UTP25 small subunit processor component (utp25), mRNA.

Price & Availability↑

CloneID	ODa37464
Clone ID Related Accession (Same CDS sequence)	NM_173273.1
Accession Version	NM_173273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Danio rerio(zebrafish)
Product	digestive organ expansion factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF506211.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF506211.1, BC058062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAAAA GAAGGCGATG TAAACAGGAG ATTGATAATT TAACTAAGAA ACAGAAGAAA 
CATCTAAAGG AATTTGGTGA ACAACACCCT TTTCACGATA AGGTTGTTGA AAGACCGGAG 
AAGACTCAGA TTTTGCGCTT GCCTGACAGT CCACAGCGCC CTGAACCAGA CAGTGAGGAT 
GACAGTGATG CTGAGCAACC ATCTGCATAT CAGAAACTAC TGTCCACCAT GATTCGAGGT 
GATGAAGATG ATGTTGAGAG TGAAGATGAA GAAAGCGAGG AAGAGGATAA CGAGGAGGAA 
GCTGAAGTTG AAGGTGATAG TGAAGAAGAA GTGGAGGACA CCGATGTAGA GGAAGGGAAT 
GATGCTGAGG AAGTTTTAGA GGATAAAGTG GAAGAAACAT CTGACAAATT ACAGAAAAAA 
CACCCTGAAA AGACCAATGG AGATGTAGAG GAGAATGAAG AGGCAGAGAT GGAGGGAGAG 
TTTACAGATA AAAAGAATGA AGCTGCTTTC TGCTTGGAGA CCAATATGCC TGCAGAAGGA 
GAGGAAAACA CAGCACAACA GGAACAGGAT GAAGACATGT TTGTGAAGCA CCAAGAGATT 
GAACTGAGCG AAGAGGAAGT GGGACGAATC TCACAAGGAT CAAAGGTCAA GACTCAGGTT 
AAGTGGGCAA AACTGGGTGT TCTGCAGTGC ATGAGTCCAT TGGAACGTTT TCCAGCCATC 
GGACAGGCCT CTTCTGCCCC TCTTCCACCC ATTCACAAAA CCCTAGAGGC AAATTGGCAT 
TTGTTAAACC TGCCTTTTGG AGCTGCAACA GATGTCACAG AGCTGCAAAA GGAGATGCTT 
GGGCTCATGG GTACTTACAG AGACCTCTAT TTGGCAAACC TCTCCCCCTT GAAGGAGGCT 
AAGGAAGTGC GCAACGCATA CTGTCTTCAT GCCCTGAACC ATGTATTAAA GGCCAACTCT 
CGTGTGCTTA GAAACAATGC CAAGTTAAAG GAATCTAAAA ATGGGGATGA GGATTTTCGT 
GACCAAGGGC TTACTAGACC AAAGGTCTTG ATTTTGGTGC CATTCAGAGA TGGAGCACTG 
AGAGTTGTCC AGACCTTCAT GACACTTCTG GAGCCCAAAG GCAAGAAGAC GGATGCCAGT 
AACAAGAAAC GCTTCAAGGA GGAATATGGA GAGGAGACGG ATGAGAAGCC ACCTAATCTG 
CAAAGGCCTG ATGACTACCA CGCTGTCTTT TCTGGAAATA TAGATGATCA TTTCAGGATA 
GGTATGTCTA TCCTGAGACA CAGCATGCGC CTATACGCTC CCTTCTATTC CTCTGACATC 
ATCATTGCTT CTCCGCTGAG TCTCCGCACA GTTCTGGGTG CTGAAGGGGA GAAGAAAAGA 
GACCATGACT TTCTGTCTTC AATCGAACTT TTGATTGTGG ATCAGGCAGA TGTTTTCCTC 
ATGCAGAACT GGGAACATTT ACTCCATGTG TTGGAGCATT TGAACCTGCA GCCGCTGGAC 
TCTCATGGTG TTGATTTCTC TCGCGTTCGC ATGTGGAACC TAAACAACTG GGCAGCTTAT 
TACAGACAGA CGCTGGTGTT CAGTGCCATA CAAGAACCTC AGATCACCAA CATCCTCACC 
AAACACTGCC ACAACTACAG AGGCCAGGTG TGCAGTAAAA CCATTCCAAA GATAGGCTCA 
ATCTGTCAGG TACTTGTGCA GCTGCCACAT GTTTTTCAGA TGTTTCACTC TGACAGCTTC 
ATGGACCAGG ATGCCAGGTC CCAGTTCTTT GTGGATAAGA TCCTGCCTCA GTACAGAGAC 
TCTGTCATGT CCGACACTTT CATCTATGTG CGTTCGTACT TTGATCTTGT ACGTCTGCGA 
AACTACATGA AGAAGGAAGA CGTCAGTTTT GTTAACATTA GCGAGTACTC TCAGAGATCA 
GAGGTGTCTC GAGCGCGACA TTACTTCCAG AAAGGAGAAA AACAGTTTCT GCTCTTCTCT 
GAGAGATTCC ACTTTTATAA GAGACACACT ATCAAGGGTA TCCACAATCT GATCTTCTAC 
GGGTTGCCCA CATACCCTCA TTTCTACAGT GAGGTGTGTA ATATGCTTCA GGCTGGAGTC 
AGAGAGGGTG GTGCTTCTGT CAGCTTCACC TGCACGGCTC TGTACTCTCG ATATGATGTG 
CACCGTCTGG CTGCCATCAC CGGGGCTGAC CGCGCCGCTC AGATGCTCCA GTCCAAAAAA 
ACCACACACC TCTTCATCAC GGGGGAGGAG AAAAACACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775380.1
CDS	43..2304
Translation	MGKRRRCKQEIDNLTKKQKKHLKEFGEQHPFHDKVVERPEKTQILRLPDSPQRPEPDSEDDSDAEQPSAYQKLLSTMIRGDEDDVESEDEESEEEDNEEEAEVEGDSEEEVEDTDVEEGNDAEEVLEDKVEETSDKLQKKHPEKTNGDVEENEEAEMEGEFTDKKNEAAFCLETNMPAEGEENTAQQEQDEDMFVKHQEIELSEEEVGRISQGSKVKTQVKWAKLGVLQCMSPLERFPAIGQASSAPLPPIHKTLEANWHLLNLPFGAATDVTELQKEMLGLMGTYRDLYLANLSPLKEAKEVRNAYCLHALNHVLKANSRVLRNNAKLKESKNGDEDFRDQGLTRPKVLILVPFRDGALRVVQTFMTLLEPKGKKTDASNKKRFKEEYGEETDEKPPNLQRPDDYHAVFSGNIDDHFRIGMSILRHSMRLYAPFYSSDIIIASPLSLRTVLGAEGEKKRDHDFLSSIELLIVDQADVFLMQNWEHLLHVLEHLNLQPLDSHGVDFSRVRMWNLNNWAAYYRQTLVFSAIQEPQITNILTKHCHNYRGQVCSKTIPKIGSICQVLVQLPHVFQMFHSDSFMDQDARSQFFVDKILPQYRDSVMSDTFIYVRSYFDLVRLRNYMKKEDVSFVNISEYSQRSEVSRARHYFQKGEKQFLLFSERFHFYKRHTIKGIHNLIFYGLPTYPHFYSEVCNMLQAGVREGGASVSFTCTALYSRYDVHRLAAITGADRAAQMLQSKKTTHLFITGEEKNT

Target ORF information:

RefSeq Version	NM_173273.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio UTP25 small subunit processor component (utp25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173273.1

ORF Insert Sequence:

ATGGGCAAAA GAAGGCGATG TAAACAGGAG ATTGATAATT TAACTAAGAA ACAGAAGAAA 
CATCTAAAGG AATTTGGTGA ACAACACCCT TTTCACGATA AGGTTGTTGA AAGACCGGAG 
AAGACTCAGA TTTTGCGCTT GCCTGACAGT CCACAGCGCC CTGAACCAGA CAGTGAGGAT 
GACAGTGATG CTGAGCAACC ATCTGCATAT CAGAAACTAC TGTCCACCAT GATTCGAGGT 
GATGAAGATG ATGTTGAGAG TGAAGATGAA GAAAGCGAGG AAGAGGATAA CGAGGAGGAA 
GCTGAAGTTG AAGGTGATAG TGAAGAAGAA GTGGAGGACA CCGATGTAGA GGAAGGGAAT 
GATGCTGAGG AAGTTTTAGA GGATAAAGTG GAAGAAACAT CTGACAAATT ACAGAAAAAA 
CACCCTGAAA AGACCAATGG AGATGTAGAG GAGAATGAAG AGGCAGAGAT GGAGGGAGAG 
TTTACAGATA AAAAGAATGA AGCTGCTTTC TGCTTGGAGA CCAATATGCC TGCAGAAGGA 
GAGGAAAACA CAGCACAACA GGAACAGGAT GAAGACATGT TTGTGAAGCA CCAAGAGATT 
GAACTGAGCG AAGAGGAAGT GGGACGAATC TCACAAGGAT CAAAGGTCAA GACTCAGGTT 
AAGTGGGCAA AACTGGGTGT TCTGCAGTGC ATGAGTCCAT TGGAACGTTT TCCAGCCATC 
GGACAGGCCT CTTCTGCCCC TCTTCCACCC ATTCACAAAA CCCTAGAGGC AAATTGGCAT 
TTGTTAAACC TGCCTTTTGG AGCTGCAACA GATGTCACAG AGCTGCAAAA GGAGATGCTT 
GGGCTCATGG GTACTTACAG AGACCTCTAT TTGGCAAACC TCTCCCCCTT GAAGGAGGCT 
AAGGAAGTGC GCAACGCATA CTGTCTTCAT GCCCTGAACC ATGTATTAAA GGCCAACTCT 
CGTGTGCTTA GAAACAATGC CAAGTTAAAG GAATCTAAAA ATGGGGATGA GGATTTTCGT 
GACCAAGGGC TTACTAGACC AAAGGTCTTG ATTTTGGTGC CATTCAGAGA TGGAGCACTG 
AGAGTTGTCC AGACCTTCAT GACACTTCTG GAGCCCAAAG GCAAGAAGAC GGATGCCAGT 
AACAAGAAAC GCTTCAAGGA GGAATATGGA GAGGAGACGG ATGAGAAGCC ACCTAATCTG 
CAAAGGCCTG ATGACTACCA CGCTGTCTTT TCTGGAAATA TAGATGATCA TTTCAGGATA 
GGTATGTCTA TCCTGAGACA CAGCATGCGC CTATACGCTC CCTTCTATTC CTCTGACATC 
ATCATTGCTT CTCCGCTGAG TCTCCGCACA GTTCTGGGTG CTGAAGGGGA GAAGAAAAGA 
GACCATGACT TTCTGTCTTC AATCGAACTT TTGATTGTGG ATCAGGCAGA TGTTTTCCTC 
ATGCAGAACT GGGAACATTT ACTCCATGTG TTGGAGCATT TGAACCTGCA GCCGCTGGAC 
TCTCATGGTG TTGATTTCTC TCGCGTTCGC ATGTGGAACC TAAACAACTG GGCAGCTTAT 
TACAGACAGA CGCTGGTGTT CAGTGCCATA CAAGAACCTC AGATCACCAA CATCCTCACC 
AAACACTGCC ACAACTACAG AGGCCAGGTG TGCAGTAAAA CCATTCCAAA GATAGGCTCA 
ATCTGTCAGG TACTTGTGCA GCTGCCACAT GTTTTTCAGA TGTTTCACTC TGACAGCTTC 
ATGGACCAGG ATGCCAGGTC CCAGTTCTTT GTGGATAAGA TCCTGCCTCA GTACAGAGAC 
TCTGTCATGT CCGACACTTT CATCTATGTG CGTTCGTACT TTGATCTTGT ACGTCTGCGA 
AACTACATGA AGAAGGAAGA CGTCAGTTTT GTTAACATTA GCGAGTACTC TCAGAGATCA 
GAGGTGTCTC GAGCGCGACA TTACTTCCAG AAAGGAGAAA AACAGTTTCT GCTCTTCTCT 
GAGAGATTCC ACTTTTATAA GAGACACACT ATCAAGGGTA TCCACAATCT GATCTTCTAC 
GGGTTGCCCA CATACCCTCA TTTCTACAGT GAGGTGTGTA ATATGCTTCA GGCTGGAGTC 
AGAGAGGGTG GTGCTTCTGT CAGCTTCACC TGCACGGCTC TGTACTCTCG ATATGATGTG 
CACCGTCTGG CTGCCATCAC CGGGGCTGAC CGCGCCGCTC AGATGCTCCA GTCCAAAAAA 
ACCACACACC TCTTCATCAC GGGGGAGGAG AAAAACACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The pre-rRNA processing factor DEF is rate limiting for the pathogenesis of MYCN-driven neuroblastoma.
Oncogene36(27)3852-3867(2017 Jul)
Tao T,Sondalle SB,Shi H,Zhu S,Perez-Atayde AR,Peng J,Baserga SJ,Look AT

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

An equation to estimate the difference between theoretically predicted and SDS PAGE-displayed molecular weights for an acidic peptide.
Scientific reports513370(2015 Aug)
Guan Y,Zhu Q,Huang D,Zhao S,Jan Lo L,Peng J

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
Cell research23(5)620-34(2013 May)
Tao T,Shi H,Guan Y,Huang D,Chen Y,Lane DP,Chen J,Peng J