sec61a1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sec61a1
Entrez Gene ID	192298
Full Name	Sec61 translocon alpha 1 subunit
Synonyms	chunp6898,fb62c11,sec61a,sec61al1,wu:fb62c11
General protein information	Preferred Names Sec61 translocon alpha 1 subunit Names protein transport protein Sec61 subunit alpha-like 1 SEC61, alpha subunit Sec61 alpha 1 subunit Sec61 alpha like 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_153659.1	NP_705945.1	protein transport protein Sec61 subunit alpha-like 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SEC61A1	XP_003310030.1
Danio rerio (zebrafish)	sec61a1	NP_705945.1
Danio rerio (zebrafish)	sec61al2	NP_963871.1
Kluyveromyces lactis	KLLA0E01145g	XP_454000.1
Bos taurus (cattle)	SEC61A1	NP_001035594.1
Gallus gallus (chicken)	SEC61A1	XP_414364.3
Saccharomyces cerevisiae (baker's yeast)	SEC61	NP_013482.1
Eremothecium gossypii	AGOS_AFR596W	NP_986143.1
Oryza sativa (rice)	Os09g0347700	NP_001062945.1
Homo sapiens (human)	SEC61A1	NP_037468.1
Rattus norvegicus (Norway rat)	Sec61a1	NP_954865.1
Schizosaccharomyces pombe (fission yeast)	sec61	NP_595226.1
Arabidopsis thaliana (thale cress)	AT1G29310	NP_174225.2
Xenopus tropicalis (tropical clawed frog)	sec61a1	NP_001004801.1
Canis lupus familiaris (dog)	SEC61A1	NP_001003315.1
Mus musculus (house mouse)	Sec61a1	NP_058602.1
Neurospora crassa	NCU08897	XP_958835.1
Arabidopsis thaliana (thale cress)	AT2G34250	NP_180972.1
Oryza sativa (rice)	Os03g0213100	NP_001049359.1
	SEC61A1	XP_002798757.1
Magnaporthe oryzae (rice blast fungus)	MGG_04856	XP_003712335.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Quantitative proteomics analysis of zebrafish exposed to sub-lethal dosages of ?-methyl-amino-L-alanine (BMAA).
Fr?yset AK, Khan EA, Fladmark KE
Scientific reports629631(2016 Jul)

Heterozygous Loss-of-Function SEC61A1 Mutations Cause Autosomal-Dominant Tubulo-Interstitial and Glomerulocystic Kidney Disease with Anemia.
Bolar NA, Golzio C, ?ivn? M, Hayot G, Van Hemelrijk C, Schepers D, Vandeweyer G, Hoischen A, Huyghe JR, Raes A, Matthys E, Sys E, Azou M, Gubler MC, Praet M, Van Camp G, McFadden K, Pediaditakis I, P?istoupilov? A, Hoda?ov? K, Vyle?al P, Hartmannov? H, Str?neck? V, H?lkov? H, Bare?ov? V, Jedli?kov? I, Sovov? J, Hn?zda A, Kidd K, Bleyer AJ, Spong RS, Vande Walle J, Mortier G, Brunner H, Van Laer L, Kmoch S, Katsanis N, Loeys BL
American journal of human genetics99(1)174-87(2016 Jul)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Investigation of the effects of estrogen on skeletal gene expression during zebrafish larval head development.
Pashay Ahi E, Walker BS, Lassiter CS, J?nsson ZO
PeerJ4e1878(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A screen for mutations that affect habenular laterality led to the identification of the sec61a-like 1(sec61al1) gene.
Title: Subnuclear development of the zebrafish habenular nuclei requires ER translocon function.

The following sec61a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sec61a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37854	NM_153659.1 Latest version!	Danio rerio SEC61 translocon subunit alpha 1 (sec61a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sec61a1 ( NM_153659.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_705945.1 Danio rerio SEC61 translocon subunit alpha 1 (sec61a1), mRNA.

Price & Availability↑

CloneID	ODa37854
Clone ID Related Accession (Same CDS sequence)	NM_153659.1
Accession Version	NM_153659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-08
Organism	Danio rerio(zebrafish)
Product	protein transport protein Sec61 subunit alpha-like 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY029527.1. ##Evidence-Data-START## Transcript exon combination :: AY029527.1, BC066715.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGATTA AGTTCTTGGA GGTAATAAAA CCGTTTTGTG CGGTTTTGCC TGAAATCCAG 
AAACCAGAAC GAAAGATCCA GTTCAGAGAA AAGGTGCTAT GGACTGCCAT CACATTGTTC 
ATCTTTCTTG TTTGCTGTCA GATCCCACTG TTTGGGATCA TGTCTTCAGA CTCAGCAGAT 
CCATTCTACT GGATGAGAGT CATCTTGGCT TCCAACAGAG GTACTTTGAT GGAGTTGGGT 
ATCTCTCCCA TTGTGACCTC TGGTCTGATC ATGCAGCTGC TGGCTGGAGC TAAAATCATT 
GAGGTCGGAG ACACACCGAA AGACAGAGCG CTCTTTAATG GAGCCCAAAA ATTGTTTGGT 
ATGATCATCA CCATTGGCCA GGCTATTGTG TACGTCATGA CTGGAATGTA TGGAGACCCT 
TCAGAGATGG GTGCTGGCAT CTGTCTGCTG ATCATCATTC AGTTGTTTGT GGCTGGTCTG 
ATCGTGTTGC TGCTGGATGA GTTGCTGCAG AAAGGTTATG GTTTGGGCTC TGGTATCTCT 
CTCTTCATTG CCACTAACAT CTGTGAGACT ATCGTATGGA AAGCATTCAG CCCAACCACA 
GTCAACACAG GCAGAGGTAC TGAGTTTGAG GGAGCCATTA TTGCTCTGTT CCACCTTTTG 
GCCACTCGCA CTGATAAAGT CAGAGCTCTG AGAGAGGCTT TCTACAGACA GAACCTGCCT 
AACCTCATGA ACCTCATCGC TACAGTCTTC GTCTTTGCTG TGGTCATATA CTTCCAGGGC 
TTCAGAGTTG ATCTGCCCAT CAAGTCTGCA CGTTACCGTG GCCAATACAA CACATATCCC 
ATCAAGCTGT TCTATACCTC CAACATTCCC ATCATCCTGC AGTCTGCTCT GGTTTCCAAT 
CTTTACGTCA TCTCTCAGAT GCTCTCCACT CGATCCAGCG GCAACTTCCT AGTCAACCTT 
CTGGGGACTT GGTCTGACAC CTCATCCGGA GGTCCAGCAC GCGCCTATCC TGTGGGCGGT 
CTCTGTTACT ATCTGTCTCC TCCGGAGTCG TTTGGTTCTG TGCTTGATGA CCCAGTTCAT 
GCAGTGATCT ACATTGTGTT CATGCTGGGA TCCTGTGCCT TCTTTTCAAA GACCTGGATT 
GAAGTTTCTG GATCTTCTGC CAAAGATGTA GCTAAGCAGC TGAAAGAACA GCAGATGGTG 
ATGAGGGGAC ACAGAGAGAC CTCCATGGTT CATGAACTCA ACAGATACAT CCCCACAGCG 
GCTGCATTTG GTGGACTGTG TATCGGAGGC CTTTCAGTAA TGGCGGACTT CCTGGGCGCC 
ATCGGCTCCG GAACGGGAAT CCTATTGGCT GTTACCATCA TCTATCAGTA CTTTGAGATA 
TTTGTTAAGG AACAGAGTGA GGTCGGCAGC ATGGGAGCGC TACTCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705945.1
CDS	126..1556
Translation	MAIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQAIVYVMTGMYGDPSEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVNTGRGTEFEGAIIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYNTYPIKLFYTSNIPIILQSALVSNLYVISQMLSTRSSGNFLVNLLGTWSDTSSGGPARAYPVGGLCYYLSPPESFGSVLDDPVHAVIYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTAAAFGGLCIGGLSVMADFLGAIGSGTGILLAVTIIYQYFEIFVKEQSEVGSMGALLF

Target ORF information:

RefSeq Version	NM_153659.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC61 translocon subunit alpha 1 (sec61a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153659.1

ORF Insert Sequence:

ATGGCGATTA AGTTCTTGGA GGTAATAAAA CCGTTTTGTG CGGTTTTGCC TGAAATCCAG 
AAACCAGAAC GAAAGATCCA GTTCAGAGAA AAGGTGCTAT GGACTGCCAT CACATTGTTC 
ATCTTTCTTG TTTGCTGTCA GATCCCACTG TTTGGGATCA TGTCTTCAGA CTCAGCAGAT 
CCATTCTACT GGATGAGAGT CATCTTGGCT TCCAACAGAG GTACTTTGAT GGAGTTGGGT 
ATCTCTCCCA TTGTGACCTC TGGTCTGATC ATGCAGCTGC TGGCTGGAGC TAAAATCATT 
GAGGTCGGAG ACACACCGAA AGACAGAGCG CTCTTTAATG GAGCCCAAAA ATTGTTTGGT 
ATGATCATCA CCATTGGCCA GGCTATTGTG TACGTCATGA CTGGAATGTA TGGAGACCCT 
TCAGAGATGG GTGCTGGCAT CTGTCTGCTG ATCATCATTC AGTTGTTTGT GGCTGGTCTG 
ATCGTGTTGC TGCTGGATGA GTTGCTGCAG AAAGGTTATG GTTTGGGCTC TGGTATCTCT 
CTCTTCATTG CCACTAACAT CTGTGAGACT ATCGTATGGA AAGCATTCAG CCCAACCACA 
GTCAACACAG GCAGAGGTAC TGAGTTTGAG GGAGCCATTA TTGCTCTGTT CCACCTTTTG 
GCCACTCGCA CTGATAAAGT CAGAGCTCTG AGAGAGGCTT TCTACAGACA GAACCTGCCT 
AACCTCATGA ACCTCATCGC TACAGTCTTC GTCTTTGCTG TGGTCATATA CTTCCAGGGC 
TTCAGAGTTG ATCTGCCCAT CAAGTCTGCA CGTTACCGTG GCCAATACAA CACATATCCC 
ATCAAGCTGT TCTATACCTC CAACATTCCC ATCATCCTGC AGTCTGCTCT GGTTTCCAAT 
CTTTACGTCA TCTCTCAGAT GCTCTCCACT CGATCCAGCG GCAACTTCCT AGTCAACCTT 
CTGGGGACTT GGTCTGACAC CTCATCCGGA GGTCCAGCAC GCGCCTATCC TGTGGGCGGT 
CTCTGTTACT ATCTGTCTCC TCCGGAGTCG TTTGGTTCTG TGCTTGATGA CCCAGTTCAT 
GCAGTGATCT ACATTGTGTT CATGCTGGGA TCCTGTGCCT TCTTTTCAAA GACCTGGATT 
GAAGTTTCTG GATCTTCTGC CAAAGATGTA GCTAAGCAGC TGAAAGAACA GCAGATGGTG 
ATGAGGGGAC ACAGAGAGAC CTCCATGGTT CATGAACTCA ACAGATACAT CCCCACAGCG 
GCTGCATTTG GTGGACTGTG TATCGGAGGC CTTTCAGTAA TGGCGGACTT CCTGGGCGCC 
ATCGGCTCCG GAACGGGAAT CCTATTGGCT GTTACCATCA TCTATCAGTA CTTTGAGATA 
TTTGTTAAGG AACAGAGTGA GGTCGGCAGC ATGGGAGCGC TACTCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Quantitative proteomics analysis of zebrafish exposed to sub-lethal dosages of ?-methyl-amino-L-alanine (BMAA).
Scientific reports629631(2016 Jul)
Fr?yset AK,Khan EA,Fladmark KE

Heterozygous Loss-of-Function SEC61A1 Mutations Cause Autosomal-Dominant Tubulo-Interstitial and Glomerulocystic Kidney Disease with Anemia.
American journal of human genetics99(1)174-87(2016 Jul)
Bolar NA,Golzio C,?ivn? M,Hayot G,Van Hemelrijk C,Schepers D,Vandeweyer G,Hoischen A,Huyghe JR,Raes A,Matthys E,Sys E,Azou M,Gubler MC,Praet M,Van Camp G,McFadden K,Pediaditakis I,P?istoupilov? A,Hoda?ov? K,Vyle?al P,Hartmannov? H,Str?neck? V,H?lkov? H,Bare?ov? V,Jedli?kov? I,Sovov? J,Hn?zda A,Kidd K,Bleyer AJ,Spong RS,Vande Walle J,Mortier G,Brunner H,Van Laer L,Kmoch S,Katsanis N,Loeys BL

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Investigation of the effects of estrogen on skeletal gene expression during zebrafish larval head development.
PeerJ4e1878(2016)
Pashay Ahi E,Walker BS,Lassiter CS,J?nsson ZO