usp48 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp48
Entrez Gene ID	100332674
Full Name	ubiquitin specific peptidase 48
Synonyms	im:7139343,zgc:112364
General protein information	Preferred Names ubiquitin specific peptidase 48 Names ubiquitin carboxyl-terminal hydrolase 48
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005172916.3	XP_005172973.1	ubiquitin carboxyl-terminal hydrolase 48
XM_005172916.4	XP_005172973.1	ubiquitin carboxyl-terminal hydrolase 48

Pathways from BioSystems

Homologies

	LOC707110	XP_002808322.1
Bos taurus (cattle)	USP48	XP_003585840.1
Mus musculus (house mouse)	Usp48	NP_570949.2
Gallus gallus (chicken)	USP48	NP_001073211.1
Xenopus tropicalis (tropical clawed frog)	usp48	NP_001120167.1
Danio rerio (zebrafish)	usp48	XP_005172974.1
Homo sapiens (human)	USP48	NP_115612.4
Pan troglodytes (chimpanzee)	USP48	XP_001163789.1
Rattus norvegicus (Norway rat)	Usp48	NP_942080.1
Canis lupus familiaris (dog)	USP48	XP_535372.2

Gene Ontology

The following usp48 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp48 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17269	XM_005172916.4 Latest version!	Danio rerio ubiquitin specific peptidase 48 (usp48), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa17269	XM_005172916.3	Danio rerio ubiquitin specific peptidase 48 (usp48), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp48 ( XM_005172916.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172973.1 Danio rerio ubiquitin specific peptidase 48 (usp48), mRNA.

Price & Availability↑

CloneID	ODa17269
Clone ID Related Accession (Same CDS sequence)	XM_005172916.3 , XM_005172916.4
Accession Version	XM_005172916.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 48
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCTC GGTTGCAGCT GGAAAAAGCT GCTTGGCGAT GGGCAGAGGC AGTCAGACCA 
GAGGACATCA CACATGAACA TATAGAGCTG GCTTATAGGA TCGCAGTGTC GGCTTGCAAA 
AGAGGGGCAT GCAGGAGGAA CTGTAAAGGA AACCCTAATT GTCTTGTTGG TATTGGGGAA 
CAATCATGGT TGGGAGAAAT TGATGAAAAC ACCTTCCACA ACATTGATGA CCCAAATTCG 
GAAAGAAGAG ATAAGAACAC ATTTGTGGGC CTGACGAACC TCGGCGCCAC GTGTTACGTC 
AACACTTTCC TCCAGGTGTG GTTCCACAAT CTGGAGCTGC GCAGAGCCTT ATATAGGTTT 
CAGAATTCCA GAGCTGAAGG GCACAACACA GATTCAGATT ATGAACCACG GACGATTTGC 
GAGCATCTCC AGTATCTGTT TGCACTGCTA CAGAACAGCA ACAGGCGATA CATCGATCCT 
TCCGGGCTAG TGAAGGCTCT TGGGCTCGAC ACAGGCCAAC AGCAGGATGC TCAGGAATTC 
TCCAAACTCT TCCTCTCACT TCTCGAAGAC ACACTTTCAA AGCAGAAGGA CCCAAATCTC 
CAAAATGTCA TTCAACAGCA GTTCTGTGGG CAGTTCTCTT ATGTTACAGT ATGCAACAAA 
TGTGGACGTG AATCTCCATT ACCATCACGA TTTTATGAAC TAGAGCTGAA TATTCAAGGA 
CATAAAAACC TCACAGAGTG TGTTACAGAG TTTCTGAAGG AGGAGAAGCT GGACGGTGAT 
AATCGTTACT ATTGCGAGAG CTGTCAGAGT AAACAGAACG CCACACGCCG GATCAAGCTG 
CAGAGTCTTC CTCGCACACT CAACTTTCAG CTCATGCGTT TCGTTTTTGA TAGGCAAAGT 
GGGCATAAGA AGAAGCTCAA CACCTTCATT AGTTTTCCAG AAGTTCTTGA CATGGAGCCG 
TTTTTGGAAG GAAGAGAGGA GAAGTGCACG TATGAGCTGA GCGCGGTGTT GATTCATCGG 
GGTGTGAGTG CTTACTCCGG TCACTACATC GCCCACGTGA GAGACGCACA CACCAACGAC 
TGGTACAAAT TTAACGATGA GGAGATCGAG AAAATGGAGG GCAAGAAACT TCAGCTGGGC 
ATTGAGGAAG ATATCGCTGA GACGGCCAAA TCTCAAACCA GAAAGCCAAA ATGTAGCAAA 
GGCTATCACT GTTCGAGGAA CGCATACATG TTAGTGTATA AACAGCAGAC AGAAGAGATT 
AACCAGACGG AGTCTCCTGT TGATGTACCA GCTTTTCTCC AGAAGCTGGT CGAGCAGGAC 
AACAGGAAGT TTGAGGAGTG GTGTAGTGAG ATGTCAGACA TGCGAAAGCA GAGTGTTGAC 
AAAGGCAAAG CCAAACATGA GGAGGTGAAG GAGCTCTACG AATTGTTACC TGCAGAAGAC 
GGCCAGTCCT ATGAGTTTGT GCCTCTAGAG TGGTTGAAGA AATGGCTTGA CGACTCCACT 
GCCATAAAGG AAATCGACAA CAGCCAGTTC CTGTGCACCC ATGGCAAACT GCATCCTGAC 
AAGATTGGGG AAGCCAAAAG AATATCCTTA AAGGCTGCTG ATCTCCTTTT CAGCCGCTAC 
GGAGGAGGAC CCAGACTAGA CCGTTCATCT CTCTGTAGAG ACTGTGTCAC TCAGCGATGT 
CGGGTGATCA GACTGAAAAA CCAGCTAAAT GAAGATTACA GAGAGGTCAC CAATCTTGCC 
AAATCCGCTT TGAAAAGTGA AGAATCAGGA TTCTGGATTG GGAAGGCTTC TTTAAGGAGC 
TGGAGACAAT TAGCATTAGA CCAGCTAGAG GAAGATGAAG AGGAAACCAA ACACAACAAC 
AGCAAAATTA ATGGAGAGAA AAGCAGCCCA GGAACTAAAG CTGATGGGGT TAAAGGAGAT 
AGTGAAGATG GAGACGGTGA AGAGATGAAG AACTTCAATG AAGATATCCT CTGTTATCAT 
GGGGGTTTGA GTATCTTAGA GAATGATCGA CGGCTGGTTT CTGCCGAGGT TTGGAATAAA 
CTAAGGATGT ATTTCCCCAA AGCACCAGAG TTCACACAAG ATCATGATCC CTGTCAGCAG 
TGCATGAGGC TGGAAAGAGA AGGGAAAGAG AACGAGGCTT TGAACAGAAT GATGGCGAAT 
GAACAGAAGA GCTCTCTTCT CAACCTCTTC CAGGAGAAGA ATCGGCCCAC GCTACAGAAA 
TGGCCACAGG ACACAGACAT TCTGTACATT GTGCCACTTT ACTTTGTTGA GGAATGGAAG 
AAATTTATTA GGAGGCCAGC AAAGGGTAAC CCAGTGTCAA ATGTGGGGAA CAGTATCCTG 
CTCTGTCCTC ACGGAGGCTT CATGTTCACA TATGACTCCA TGCTGCAAGG AGACGCCCAA 
CATATAGCTC TTCTCTGGCC CGCTGAATGG GAGGTGATCA GCAAAATGTT TCTGGTGGAC 
CAGGTCATCT CCATCTGTCG GATTCATGAC AAAACACAAG ACAACGGCAA TGTGCAATAC 
CAGACTCATC CAGATCTGTG TCGGGAATGC AGAGAGGGCT TCATATTCCA ACAGCAGAGG 
GACATGAGGG AGTACACACA GGCCACCGTG TATGTGCGCA AAGTCATCGA CAAGAAAAGA 
ATGATTAAGG AATCTGCCCC TGAGTTTAGT GTTAGTGGAT CAGATGTTGA AGATGAAAAA 
GAGGAGCCAA AATTAGATGG AGAGAAAGAT CCAGACTTCA GTCAGACTGA GGGTGGAGCC 
AAAAGGCAGA AGCTGAACGA TACTGTCTCT CTTCCTACGG CTGTCGTAAC CACCACATCT 
AAGTCAGGCA TCAGGAGGAG CACAAGGCAC AGAAAACTGC GTGGCGAAAA AGCCCTCATC 
GTTTCTGCCA ATCAGACGCT CAAAGATCTC AAGATCCAGA TCATGCACGC GTTCTCAGTG 
GCTCCATTCG ATCAAAACCT TTCCATAGAC GGACGCTGTT TAAAAGACGA CTCTGCCACA 
TTGGGAAGTC TTGGAGTCAT ACCTGAGAGC ATCATTTGCC TTAAGGCTGA TGAACCCATT 
GCTGATTATG CTGCAATGGA TGATGTTTAT CAAGTCTGTA TGCCTGAAGA AGGATTTAAA 
GGCACTGGGC TTTTAGGACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172973.1
CDS	349..3492
Translation	MAPRLQLEKAAWRWAEAVRPEDITHEHIELAYRIAVSACKRGACRRNCKGNPNCLVGIGEQSWLGEIDENTFHNIDDPNSERRDKNTFVGLTNLGATCYVNTFLQVWFHNLELRRALYRFQNSRAEGHNTDSDYEPRTICEHLQYLFALLQNSNRRYIDPSGLVKALGLDTGQQQDAQEFSKLFLSLLEDTLSKQKDPNLQNVIQQQFCGQFSYVTVCNKCGRESPLPSRFYELELNIQGHKNLTECVTEFLKEEKLDGDNRYYCESCQSKQNATRRIKLQSLPRTLNFQLMRFVFDRQSGHKKKLNTFISFPEVLDMEPFLEGREEKCTYELSAVLIHRGVSAYSGHYIAHVRDAHTNDWYKFNDEEIEKMEGKKLQLGIEEDIAETAKSQTRKPKCSKGYHCSRNAYMLVYKQQTEEINQTESPVDVPAFLQKLVEQDNRKFEEWCSEMSDMRKQSVDKGKAKHEEVKELYELLPAEDGQSYEFVPLEWLKKWLDDSTAIKEIDNSQFLCTHGKLHPDKIGEAKRISLKAADLLFSRYGGGPRLDRSSLCRDCVTQRCRVIRLKNQLNEDYREVTNLAKSALKSEESGFWIGKASLRSWRQLALDQLEEDEEETKHNNSKINGEKSSPGTKADGVKGDSEDGDGEEMKNFNEDILCYHGGLSILENDRRLVSAEVWNKLRMYFPKAPEFTQDHDPCQQCMRLEREGKENEALNRMMANEQKSSLLNLFQEKNRPTLQKWPQDTDILYIVPLYFVEEWKKFIRRPAKGNPVSNVGNSILLCPHGGFMFTYDSMLQGDAQHIALLWPAEWEVISKMFLVDQVISICRIHDKTQDNGNVQYQTHPDLCRECREGFIFQQQRDMREYTQATVYVRKVIDKKRMIKESAPEFSVSGSDVEDEKEEPKLDGEKDPDFSQTEGGAKRQKLNDTVSLPTAVVTTTSKSGIRRSTRHRKLRGEKALIVSANQTLKDLKIQIMHAFSVAPFDQNLSIDGRCLKDDSATLGSLGVIPESIICLKADEPIADYAAMDDVYQVCMPEEGFKGTGLLGH

Target ORF information:

RefSeq Version	XM_005172916.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 48 (usp48), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172916.3

ORF Insert Sequence:

ATGGCTCCTC GGTTGCAGCT GGAAAAAGCT GCTTGGCGAT GGGCAGAGGC AGTCAGACCA 
GAGGACATCA CACATGAACA TATAGAGCTG GCTTATAGGA TCGCAGTGTC GGCTTGCAAA 
AGAGGGGCAT GCAGGAGGAA CTGTAAAGGA AACCCTAATT GTCTTGTTGG TATTGGGGAA 
CAATCATGGT TGGGAGAAAT TGATGAAAAC ACCTTCCACA ACATTGATGA CCCAAATTCG 
GAAAGAAGAG ATAAGAACAC ATTTGTGGGC CTGACGAACC TCGGCGCCAC GTGTTACGTC 
AACACTTTCC TCCAGGTGTG GTTCCACAAT CTGGAGCTGC GCAGAGCCTT ATATAGGTTT 
CAGAATTCCA GAGCTGAAGG GCACAACACA GATTCAGATT ATGAACCACG GACGATTTGC 
GAGCATCTCC AGTATCTGTT TGCACTGCTA CAGAACAGCA ACAGGCGATA CATCGATCCT 
TCCGGGCTAG TGAAGGCTCT TGGGCTCGAC ACAGGCCAAC AGCAGGATGC TCAGGAATTC 
TCCAAACTCT TCCTCTCACT TCTCGAAGAC ACACTTTCAA AGCAGAAGGA CCCAAATCTC 
CAAAATGTCA TTCAACAGCA GTTCTGTGGG CAGTTCTCTT ATGTTACAGT ATGCAACAAA 
TGTGGACGTG AATCTCCATT ACCATCACGA TTTTATGAAC TAGAGCTGAA TATTCAAGGA 
CATAAAAACC TCACAGAGTG TGTTACAGAG TTTCTGAAGG AGGAGAAGCT GGACGGTGAT 
AATCGTTACT ATTGCGAGAG CTGTCAGAGT AAACAGAACG CCACACGCCG GATCAAGCTG 
CAGAGTCTTC CTCGCACACT CAACTTTCAG CTCATGCGTT TCGTTTTTGA TAGGCAAAGT 
GGGCATAAGA AGAAGCTCAA CACCTTCATT AGTTTTCCAG AAGTTCTTGA CATGGAGCCG 
TTTTTGGAAG GAAGAGAGGA GAAGTGCACG TATGAGCTGA GCGCGGTGTT GATTCATCGG 
GGTGTGAGTG CTTACTCCGG TCACTACATC GCCCACGTGA GAGACGCACA CACCAACGAC 
TGGTACAAAT TTAACGATGA GGAGATCGAG AAAATGGAGG GCAAGAAACT TCAGCTGGGC 
ATTGAGGAAG ATATCGCTGA GACGGCCAAA TCTCAAACCA GAAAGCCAAA ATGTAGCAAA 
GGCTATCACT GTTCGAGGAA CGCATACATG TTAGTGTATA AACAGCAGAC AGAAGAGATT 
AACCAGACGG AGTCTCCTGT TGATGTACCA GCTTTTCTCC AGAAGCTGGT CGAGCAGGAC 
AACAGGAAGT TTGAGGAGTG GTGTAGTGAG ATGTCAGACA TGCGAAAGCA GAGTGTTGAC 
AAAGGCAAAG CCAAACATGA GGAGGTGAAG GAGCTCTACG AATTGTTACC TGCAGAAGAC 
GGCCAGTCCT ATGAGTTTGT GCCTCTAGAG TGGTTGAAGA AATGGCTTGA CGACTCCACT 
GCCATAAAGG AAATCGACAA CAGCCAGTTC CTGTGCACCC ATGGCAAACT GCATCCTGAC 
AAGATTGGGG AAGCCAAAAG AATATCCTTA AAGGCTGCTG ATCTCCTTTT CAGCCGCTAC 
GGAGGAGGAC CCAGACTAGA CCGTTCATCT CTCTGTAGAG ACTGTGTCAC TCAGCGATGT 
CGGGTGATCA GACTGAAAAA CCAGCTAAAT GAAGATTACA GAGAGGTCAC CAATCTTGCC 
AAATCCGCTT TGAAAAGTGA AGAATCAGGA TTCTGGATTG GGAAGGCTTC TTTAAGGAGC 
TGGAGACAAT TAGCATTAGA CCAGCTAGAG GAAGATGAAG AGGAAACCAA ACACAACAAC 
AGCAAAATTA ATGGAGAGAA AAGCAGCCCA GGAACTAAAG CTGATGGGGT TAAAGGAGAT 
AGTGAAGATG GAGACGGTGA AGAGATGAAG AACTTCAATG AAGATATCCT CTGTTATCAT 
GGGGGTTTGA GTATCTTAGA GAATGATCGA CGGCTGGTTT CTGCCGAGGT TTGGAATAAA 
CTAAGGATGT ATTTCCCCAA AGCACCAGAG TTCACACAAG ATCATGATCC CTGTCAGCAG 
TGCATGAGGC TGGAAAGAGA AGGGAAAGAG AACGAGGCTT TGAACAGAAT GATGGCGAAT 
GAACAGAAGA GCTCTCTTCT CAACCTCTTC CAGGAGAAGA ATCGGCCCAC GCTACAGAAA 
TGGCCACAGG ACACAGACAT TCTGTACATT GTGCCACTTT ACTTTGTTGA GGAATGGAAG 
AAATTTATTA GGAGGCCAGC AAAGGGTAAC CCAGTGTCAA ATGTGGGGAA CAGTATCCTG 
CTCTGTCCTC ACGGAGGCTT CATGTTCACA TATGACTCCA TGCTGCAAGG AGACGCCCAA 
CATATAGCTC TTCTCTGGCC CGCTGAATGG GAGGTGATCA GCAAAATGTT TCTGGTGGAC 
CAGGTCATCT CCATCTGTCG GATTCATGAC AAAACACAAG ACAACGGCAA TGTGCAATAC 
CAGACTCATC CAGATCTGTG TCGGGAATGC AGAGAGGGCT TCATATTCCA ACAGCAGAGG 
GACATGAGGG AGTACACACA GGCCACCGTG TATGTGCGCA AAGTCATCGA CAAGAAAAGA 
ATGATTAAGG AATCTGCCCC TGAGTTTAGT GTTAGTGGAT CAGATGTTGA AGATGAAAAA 
GAGGAGCCAA AATTAGATGG AGAGAAAGAT CCAGACTTCA GTCAGACTGA GGGTGGAGCC 
AAAAGGCAGA AGCTGAACGA TACTGTCTCT CTTCCTACGG CTGTCGTAAC CACCACATCT 
AAGTCAGGCA TCAGGAGGAG CACAAGGCAC AGAAAACTGC GTGGCGAAAA AGCCCTCATC 
GTTTCTGCCA ATCAGACGCT CAAAGATCTC AAGATCCAGA TCATGCACGC GTTCTCAGTG 
GCTCCATTCG ATCAAAACCT TTCCATAGAC GGACGCTGTT TAAAAGACGA CTCTGCCACA 
TTGGGAAGTC TTGGAGTCAT ACCTGAGAGC ATCATTTGCC TTAAGGCTGA TGAACCCATT 
GCTGATTATG CTGCAATGGA TGATGTTTAT CAAGTCTGTA TGCCTGAAGA AGGATTTAAA 
GGCACTGGGC TTTTAGGACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp48 ( XM_005172916.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172973.1 Danio rerio ubiquitin specific peptidase 48 (usp48), mRNA.

Price & Availability↑

CloneID	ODa17269
Clone ID Related Accession (Same CDS sequence)	XM_005172916.3 , XM_005172916.4
Accession Version	XM_005172916.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 48
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172916.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCTC GGTTGCAGCT GGAAAAAGCT GCTTGGCGAT GGGCAGAGGC AGTCAGACCA 
GAGGACATCA CACATGAACA TATAGAGCTG GCTTATAGGA TCGCAGTGTC GGCTTGCAAA 
AGAGGGGCAT GCAGGAGGAA CTGTAAAGGA AACCCTAATT GTCTTGTTGG TATTGGGGAA 
CAATCATGGT TGGGAGAAAT TGATGAAAAC ACCTTCCACA ACATTGATGA CCCAAATTCG 
GAAAGAAGAG ATAAGAACAC ATTTGTGGGC CTGACGAACC TCGGCGCCAC GTGTTACGTC 
AACACTTTCC TCCAGGTGTG GTTCCACAAT CTGGAGCTGC GCAGAGCCTT ATATAGGTTT 
CAGAATTCCA GAGCTGAAGG GCACAACACA GATTCAGATT ATGAACCACG GACGATTTGC 
GAGCATCTCC AGTATCTGTT TGCACTGCTA CAGAACAGCA ACAGGCGATA CATCGATCCT 
TCCGGGCTAG TGAAGGCTCT TGGGCTCGAC ACAGGCCAAC AGCAGGATGC TCAGGAATTC 
TCCAAACTCT TCCTCTCACT TCTCGAAGAC ACACTTTCAA AGCAGAAGGA CCCAAATCTC 
CAAAATGTCA TTCAACAGCA GTTCTGTGGG CAGTTCTCTT ATGTTACAGT ATGCAACAAA 
TGTGGACGTG AATCTCCATT ACCATCACGA TTTTATGAAC TAGAGCTGAA TATTCAAGGA 
CATAAAAACC TCACAGAGTG TGTTACAGAG TTTCTGAAGG AGGAGAAGCT GGACGGTGAT 
AATCGTTACT ATTGCGAGAG CTGTCAGAGT AAACAGAACG CCACACGCCG GATCAAGCTG 
CAGAGTCTTC CTCGCACACT CAACTTTCAG CTCATGCGTT TCGTTTTTGA TAGGCAAAGT 
GGGCATAAGA AGAAGCTCAA CACCTTCATT AGTTTTCCAG AAGTTCTTGA CATGGAGCCG 
TTTTTGGAAG GAAGAGAGGA GAAGTGCACG TATGAGCTGA GCGCGGTGTT GATTCATCGG 
GGTGTGAGTG CTTACTCCGG TCACTACATC GCCCACGTGA GAGACGCACA CACCAACGAC 
TGGTACAAAT TTAACGATGA GGAGATCGAG AAAATGGAGG GCAAGAAACT TCAGCTGGGC 
ATTGAGGAAG ATATCGCTGA GACGGCCAAA TCTCAAACCA GAAAGCCAAA ATGTAGCAAA 
GGCTATCACT GTTCGAGGAA CGCATACATG TTAGTGTATA AACAGCAGAC AGAAGAGATT 
AACCAGACGG AGTCTCCTGT TGATGTACCA GCTTTTCTCC AGAAGCTGGT CGAGCAGGAC 
AACAGGAAGT TTGAGGAGTG GTGTAGTGAG ATGTCAGACA TGCGAAAGCA GAGTGTTGAC 
AAAGGCAAAG CCAAACATGA GGAGGTGAAG GAGCTCTACG AATTGTTACC TGCAGAAGAC 
GGCCAGTCCT ATGAGTTTGT GCCTCTAGAG TGGTTGAAGA AATGGCTTGA CGACTCCACT 
GCCATAAAGG AAATCGACAA CAGCCAGTTC CTGTGCACCC ATGGCAAACT GCATCCTGAC 
AAGATTGGGG AAGCCAAAAG AATATCCTTA AAGGCTGCTG ATCTCCTTTT CAGCCGCTAC 
GGAGGAGGAC CCAGACTAGA CCGTTCATCT CTCTGTAGAG ACTGTGTCAC TCAGCGATGT 
CGGGTGATCA GACTGAAAAA CCAGCTAAAT GAAGATTACA GAGAGGTCAC CAATCTTGCC 
AAATCCGCTT TGAAAAGTGA AGAATCAGGA TTCTGGATTG GGAAGGCTTC TTTAAGGAGC 
TGGAGACAAT TAGCATTAGA CCAGCTAGAG GAAGATGAAG AGGAAACCAA ACACAACAAC 
AGCAAAATTA ATGGAGAGAA AAGCAGCCCA GGAACTAAAG CTGATGGGGT TAAAGGAGAT 
AGTGAAGATG GAGACGGTGA AGAGATGAAG AACTTCAATG AAGATATCCT CTGTTATCAT 
GGGGGTTTGA GTATCTTAGA GAATGATCGA CGGCTGGTTT CTGCCGAGGT TTGGAATAAA 
CTAAGGATGT ATTTCCCCAA AGCACCAGAG TTCACACAAG ATCATGATCC CTGTCAGCAG 
TGCATGAGGC TGGAAAGAGA AGGGAAAGAG AACGAGGCTT TGAACAGAAT GATGGCGAAT 
GAACAGAAGA GCTCTCTTCT CAACCTCTTC CAGGAGAAGA ATCGGCCCAC GCTACAGAAA 
TGGCCACAGG ACACAGACAT TCTGTACATT GTGCCACTTT ACTTTGTTGA GGAATGGAAG 
AAATTTATTA GGAGGCCAGC AAAGGGTAAC CCAGTGTCAA ATGTGGGGAA CAGTATCCTG 
CTCTGTCCTC ACGGAGGCTT CATGTTCACA TATGACTCCA TGCTGCAAGG AGACGCCCAA 
CATATAGCTC TTCTCTGGCC CGCTGAATGG GAGGTGATCA GCAAAATGTT TCTGGTGGAC 
CAGGTCATCT CCATCTGTCG GATTCATGAC AAAACACAAG ACAACGGCAA TGTGCAATAC 
CAGACTCATC CAGATCTGTG TCGGGAATGC AGAGAGGGCT TCATATTCCA ACAGCAGAGG 
GACATGAGGG AGTACACACA GGCCACCGTG TATGTGCGCA AAGTCATCGA CAAGAAAAGA 
ATGATTAAGG AATCTGCCCC TGAGTTTAGT GTTAGTGGAT CAGATGTTGA AGATGAAAAA 
GAGGAGCCAA AATTAGATGG AGAGAAAGAT CCAGACTTCA GTCAGACTGA GGGTGGAGCC 
AAAAGGCAGA AGCTGAACGA TACTGTCTCT CTTCCTACGG CTGTCGTAAC CACCACATCT 
AAGTCAGGCA TCAGGAGGAG CACAAGGCAC AGAAAACTGC GTGGCGAAAA AGCCCTCATC 
GTTTCTGCCA ATCAGACGCT CAAAGATCTC AAGATCCAGA TCATGCACGC GTTCTCAGTG 
GCTCCATTCG ATCAAAACCT TTCCATAGAC GGACGCTGTT TAAAAGACGA CTCTGCCACA 
TTGGGAAGTC TTGGAGTCAT ACCTGAGAGC ATCATTTGCC TTAAGGCTGA TGAACCCATT 
GCTGATTATG CTGCAATGGA TGATGTTTAT CAAGTCTGTA TGCCTGAAGA AGGATTTAAA 
GGCACTGGGC TTTTAGGACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172973.1
CDS	359..3502
Translation	MAPRLQLEKAAWRWAEAVRPEDITHEHIELAYRIAVSACKRGACRRNCKGNPNCLVGIGEQSWLGEIDENTFHNIDDPNSERRDKNTFVGLTNLGATCYVNTFLQVWFHNLELRRALYRFQNSRAEGHNTDSDYEPRTICEHLQYLFALLQNSNRRYIDPSGLVKALGLDTGQQQDAQEFSKLFLSLLEDTLSKQKDPNLQNVIQQQFCGQFSYVTVCNKCGRESPLPSRFYELELNIQGHKNLTECVTEFLKEEKLDGDNRYYCESCQSKQNATRRIKLQSLPRTLNFQLMRFVFDRQSGHKKKLNTFISFPEVLDMEPFLEGREEKCTYELSAVLIHRGVSAYSGHYIAHVRDAHTNDWYKFNDEEIEKMEGKKLQLGIEEDIAETAKSQTRKPKCSKGYHCSRNAYMLVYKQQTEEINQTESPVDVPAFLQKLVEQDNRKFEEWCSEMSDMRKQSVDKGKAKHEEVKELYELLPAEDGQSYEFVPLEWLKKWLDDSTAIKEIDNSQFLCTHGKLHPDKIGEAKRISLKAADLLFSRYGGGPRLDRSSLCRDCVTQRCRVIRLKNQLNEDYREVTNLAKSALKSEESGFWIGKASLRSWRQLALDQLEEDEEETKHNNSKINGEKSSPGTKADGVKGDSEDGDGEEMKNFNEDILCYHGGLSILENDRRLVSAEVWNKLRMYFPKAPEFTQDHDPCQQCMRLEREGKENEALNRMMANEQKSSLLNLFQEKNRPTLQKWPQDTDILYIVPLYFVEEWKKFIRRPAKGNPVSNVGNSILLCPHGGFMFTYDSMLQGDAQHIALLWPAEWEVISKMFLVDQVISICRIHDKTQDNGNVQYQTHPDLCRECREGFIFQQQRDMREYTQATVYVRKVIDKKRMIKESAPEFSVSGSDVEDEKEEPKLDGEKDPDFSQTEGGAKRQKLNDTVSLPTAVVTTTSKSGIRRSTRHRKLRGEKALIVSANQTLKDLKIQIMHAFSVAPFDQNLSIDGRCLKDDSATLGSLGVIPESIICLKADEPIADYAAMDDVYQVCMPEEGFKGTGLLGH

Target ORF information:

RefSeq Version	XM_005172916.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 48 (usp48), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172916.4

ORF Insert Sequence:

ATGGCTCCTC GGTTGCAGCT GGAAAAAGCT GCTTGGCGAT GGGCAGAGGC AGTCAGACCA 
GAGGACATCA CACATGAACA TATAGAGCTG GCTTATAGGA TCGCAGTGTC GGCTTGCAAA 
AGAGGGGCAT GCAGGAGGAA CTGTAAAGGA AACCCTAATT GTCTTGTTGG TATTGGGGAA 
CAATCATGGT TGGGAGAAAT TGATGAAAAC ACCTTCCACA ACATTGATGA CCCAAATTCG 
GAAAGAAGAG ATAAGAACAC ATTTGTGGGC CTGACGAACC TCGGCGCCAC GTGTTACGTC 
AACACTTTCC TCCAGGTGTG GTTCCACAAT CTGGAGCTGC GCAGAGCCTT ATATAGGTTT 
CAGAATTCCA GAGCTGAAGG GCACAACACA GATTCAGATT ATGAACCACG GACGATTTGC 
GAGCATCTCC AGTATCTGTT TGCACTGCTA CAGAACAGCA ACAGGCGATA CATCGATCCT 
TCCGGGCTAG TGAAGGCTCT TGGGCTCGAC ACAGGCCAAC AGCAGGATGC TCAGGAATTC 
TCCAAACTCT TCCTCTCACT TCTCGAAGAC ACACTTTCAA AGCAGAAGGA CCCAAATCTC 
CAAAATGTCA TTCAACAGCA GTTCTGTGGG CAGTTCTCTT ATGTTACAGT ATGCAACAAA 
TGTGGACGTG AATCTCCATT ACCATCACGA TTTTATGAAC TAGAGCTGAA TATTCAAGGA 
CATAAAAACC TCACAGAGTG TGTTACAGAG TTTCTGAAGG AGGAGAAGCT GGACGGTGAT 
AATCGTTACT ATTGCGAGAG CTGTCAGAGT AAACAGAACG CCACACGCCG GATCAAGCTG 
CAGAGTCTTC CTCGCACACT CAACTTTCAG CTCATGCGTT TCGTTTTTGA TAGGCAAAGT 
GGGCATAAGA AGAAGCTCAA CACCTTCATT AGTTTTCCAG AAGTTCTTGA CATGGAGCCG 
TTTTTGGAAG GAAGAGAGGA GAAGTGCACG TATGAGCTGA GCGCGGTGTT GATTCATCGG 
GGTGTGAGTG CTTACTCCGG TCACTACATC GCCCACGTGA GAGACGCACA CACCAACGAC 
TGGTACAAAT TTAACGATGA GGAGATCGAG AAAATGGAGG GCAAGAAACT TCAGCTGGGC 
ATTGAGGAAG ATATCGCTGA GACGGCCAAA TCTCAAACCA GAAAGCCAAA ATGTAGCAAA 
GGCTATCACT GTTCGAGGAA CGCATACATG TTAGTGTATA AACAGCAGAC AGAAGAGATT 
AACCAGACGG AGTCTCCTGT TGATGTACCA GCTTTTCTCC AGAAGCTGGT CGAGCAGGAC 
AACAGGAAGT TTGAGGAGTG GTGTAGTGAG ATGTCAGACA TGCGAAAGCA GAGTGTTGAC 
AAAGGCAAAG CCAAACATGA GGAGGTGAAG GAGCTCTACG AATTGTTACC TGCAGAAGAC 
GGCCAGTCCT ATGAGTTTGT GCCTCTAGAG TGGTTGAAGA AATGGCTTGA CGACTCCACT 
GCCATAAAGG AAATCGACAA CAGCCAGTTC CTGTGCACCC ATGGCAAACT GCATCCTGAC 
AAGATTGGGG AAGCCAAAAG AATATCCTTA AAGGCTGCTG ATCTCCTTTT CAGCCGCTAC 
GGAGGAGGAC CCAGACTAGA CCGTTCATCT CTCTGTAGAG ACTGTGTCAC TCAGCGATGT 
CGGGTGATCA GACTGAAAAA CCAGCTAAAT GAAGATTACA GAGAGGTCAC CAATCTTGCC 
AAATCCGCTT TGAAAAGTGA AGAATCAGGA TTCTGGATTG GGAAGGCTTC TTTAAGGAGC 
TGGAGACAAT TAGCATTAGA CCAGCTAGAG GAAGATGAAG AGGAAACCAA ACACAACAAC 
AGCAAAATTA ATGGAGAGAA AAGCAGCCCA GGAACTAAAG CTGATGGGGT TAAAGGAGAT 
AGTGAAGATG GAGACGGTGA AGAGATGAAG AACTTCAATG AAGATATCCT CTGTTATCAT 
GGGGGTTTGA GTATCTTAGA GAATGATCGA CGGCTGGTTT CTGCCGAGGT TTGGAATAAA 
CTAAGGATGT ATTTCCCCAA AGCACCAGAG TTCACACAAG ATCATGATCC CTGTCAGCAG 
TGCATGAGGC TGGAAAGAGA AGGGAAAGAG AACGAGGCTT TGAACAGAAT GATGGCGAAT 
GAACAGAAGA GCTCTCTTCT CAACCTCTTC CAGGAGAAGA ATCGGCCCAC GCTACAGAAA 
TGGCCACAGG ACACAGACAT TCTGTACATT GTGCCACTTT ACTTTGTTGA GGAATGGAAG 
AAATTTATTA GGAGGCCAGC AAAGGGTAAC CCAGTGTCAA ATGTGGGGAA CAGTATCCTG 
CTCTGTCCTC ACGGAGGCTT CATGTTCACA TATGACTCCA TGCTGCAAGG AGACGCCCAA 
CATATAGCTC TTCTCTGGCC CGCTGAATGG GAGGTGATCA GCAAAATGTT TCTGGTGGAC 
CAGGTCATCT CCATCTGTCG GATTCATGAC AAAACACAAG ACAACGGCAA TGTGCAATAC 
CAGACTCATC CAGATCTGTG TCGGGAATGC AGAGAGGGCT TCATATTCCA ACAGCAGAGG 
GACATGAGGG AGTACACACA GGCCACCGTG TATGTGCGCA AAGTCATCGA CAAGAAAAGA 
ATGATTAAGG AATCTGCCCC TGAGTTTAGT GTTAGTGGAT CAGATGTTGA AGATGAAAAA 
GAGGAGCCAA AATTAGATGG AGAGAAAGAT CCAGACTTCA GTCAGACTGA GGGTGGAGCC 
AAAAGGCAGA AGCTGAACGA TACTGTCTCT CTTCCTACGG CTGTCGTAAC CACCACATCT 
AAGTCAGGCA TCAGGAGGAG CACAAGGCAC AGAAAACTGC GTGGCGAAAA AGCCCTCATC 
GTTTCTGCCA ATCAGACGCT CAAAGATCTC AAGATCCAGA TCATGCACGC GTTCTCAGTG 
GCTCCATTCG ATCAAAACCT TTCCATAGAC GGACGCTGTT TAAAAGACGA CTCTGCCACA 
TTGGGAAGTC TTGGAGTCAT ACCTGAGAGC ATCATTTGCC TTAAGGCTGA TGAACCCATT 
GCTGATTATG CTGCAATGGA TGATGTTTAT CAAGTCTGTA TGCCTGAAGA AGGATTTAAA 
GGCACTGGGC TTTTAGGACA CTGA