usp46 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp46
Entrez Gene ID	100148916
Full Name	ubiquitin specific peptidase 46
Synonyms	si:ch73-16a12.1
General protein information	Preferred Names ubiquitin specific peptidase 46 Names ubiquitin carboxyl-terminal hydrolase 46 deubiquitinating enzyme 46 ubiquitin thioesterase 46 ubiquitin thiolesterase 46 ubiquitin-specific-processing protease 46
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001244981.1	NP_001231910.1	ubiquitin carboxyl-terminal hydrolase 46

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Usp46	NP_001178525.1
Drosophila melanogaster (fruit fly)	CG7023	NP_651099.1
Saccharomyces cerevisiae (baker's yeast)	UBP13	NP_009486.3
Eremothecium gossypii	AGOS_AGR370W	NP_987036.2
Bos taurus (cattle)	USP46	NP_001179373.2
Gallus gallus (chicken)	USP46	XP_420711.1
Danio rerio (zebrafish)	usp46	NP_001231910.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012139	XP_320388.3
Xenopus tropicalis (tropical clawed frog)	usp46	NP_001106637.1
Homo sapiens (human)	USP46	NP_073743.2
	USP46	XP_001092352.1
Mus musculus (house mouse)	Usp46	NP_808229.1
Arabidopsis thaliana (thale cress)	UBP4	NP_565532.1
Oryza sativa (rice)	Os04g0452400	NP_001052951.1
Pan troglodytes (chimpanzee)	USP46	XP_517296.2
Canis lupus familiaris (dog)	USP46	XP_005628243.1
Caenorhabditis elegans (roundworm)	usp-46	NP_499162.2
Saccharomyces cerevisiae (baker's yeast)	UBP9	NP_011024.1
Kluyveromyces lactis	KLLA0E20351g	XP_454871.1
Arabidopsis thaliana (thale cress)	UBP3	NP_568074.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following usp46 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp46 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39074	NM_001244981.1 Latest version!	Danio rerio ubiquitin specific peptidase 46 (usp46), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp46 ( NM_001244981.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001231910.1 Danio rerio ubiquitin specific peptidase 46 (usp46), mRNA.

Price & Availability↑

CloneID	ODa39074
Clone ID Related Accession (Same CDS sequence)	NM_001244981.1
Accession Version	NM_001244981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 46
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU693368.7 and CT827859.6. On Oct 7, 2011 this sequence version replaced XM_003200700.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01011271.1, GFIL01042611.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA4476731 [ECO:0000348] ##Evidence-Data-END##

ATGACTGTCA GAAACATCGC CTCCATTTGT AATATGGGCA CCAATGCCTC TGCTCTGGAG 
AAAGACATCG GTCCAGAGCA GTTCCCTATT AATGAACACT ATTTTGGATT GGTCAACTTT 
GGAAACACAT GCTATTGCAA CTCGGTGTTG CAGGCTCTGT ATTTCTGCCG GCCGTTCAGG 
GAGAATGTGT TGGCGTATAA AGTCCAGCAG AAGAAGAAGG AGAATCTTCT CACCTGTCTG 
GCCGACCTCT TCCACAGCAT CGCCACGCAG AAGAAGAAGG TGGGCGTGAT TCCTCCCAAG 
AAGTTCATCT CACGGCTGCG CAAGGAAAAT GACCTGTTTG ATAACTACAT GCAGCAAGAT 
GCGCACGAGT TCCTGAATTA CCTGTTGAAC ACGGTGGCTG ATATCCTGCA GGAGGAGAGA 
AAACAGGAGA AGCAGAACGG ACGACTGAAG AACAACGGCA CTGCCATCGC CACAGACACA 
GAGCCGGAGC AGAACAAAAT CGACCCAACC TGGGTTCATG AGATCTTCCA GGGCACACTG 
ACCAATGAGA CGCGCTGTCT CAACTGTGAG ACGGTCAGTA GCAAGGATGA AGACTTCTTG 
GACTTGTCTG TAGATGTTGA GCAGAACACT TCAATAACAC ACTGTCTCAG GGACTTCAGT 
AATACAGAGA CCCTGTGCAG CGAGTATAAA TACTACTGTG AGATGTGCTG TAGCAAACAA 
GAGGCACAGA AACGTATGCG TGTGAAGAAG TTGCCTATGA TCTTGGCTCT TCATCTGAAG 
CGCTTTAAGT ACATGGAGCA GCTTCATCGG TACACTAAAC TGAGTTATCG TGTGGTCTTT 
CCACTGGAGC TACGCCTCTT CAACACCTCC GGGGACGCCG TCAATCTCGA CCGCATGTAC 
GACCTCGTCG CTGTGGTGGT CCACTGTGGC AGTGGCCCAA ACAGAGGGCA TTACATCACC 
ATCGTGAAGA GCCATGGCTT CTGGCTGCTG TTTGATGACG ACATTGTGGA GAAAATAGAT 
GCGCAGGCGA TAGAGGAATT CTACGGTTTG ACCTCTGACA TTTCCAAGAA CTCTGAGTCG 
GGATACATTT TGTTTTATCA GTCCAGAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231910.1
CDS	27..1139
Translation	MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKVQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTVADILQEERKQEKQNGRLKNNGTAIATDTEPEQNKIDPTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEYKYYCEMCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSGDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE

Target ORF information:

RefSeq Version	NM_001244981.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 46 (usp46), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244981.1

ORF Insert Sequence:

ATGACTGTCA GAAACATCGC CTCCATTTGT AATATGGGCA CCAATGCCTC TGCTCTGGAG 
AAAGACATCG GTCCAGAGCA GTTCCCTATT AATGAACACT ATTTTGGATT GGTCAACTTT 
GGAAACACAT GCTATTGCAA CTCGGTGTTG CAGGCTCTGT ATTTCTGCCG GCCGTTCAGG 
GAGAATGTGT TGGCGTATAA AGTCCAGCAG AAGAAGAAGG AGAATCTTCT CACCTGTCTG 
GCCGACCTCT TCCACAGCAT CGCCACGCAG AAGAAGAAGG TGGGCGTGAT TCCTCCCAAG 
AAGTTCATCT CACGGCTGCG CAAGGAAAAT GACCTGTTTG ATAACTACAT GCAGCAAGAT 
GCGCACGAGT TCCTGAATTA CCTGTTGAAC ACGGTGGCTG ATATCCTGCA GGAGGAGAGA 
AAACAGGAGA AGCAGAACGG ACGACTGAAG AACAACGGCA CTGCCATCGC CACAGACACA 
GAGCCGGAGC AGAACAAAAT CGACCCAACC TGGGTTCATG AGATCTTCCA GGGCACACTG 
ACCAATGAGA CGCGCTGTCT CAACTGTGAG ACGGTCAGTA GCAAGGATGA AGACTTCTTG 
GACTTGTCTG TAGATGTTGA GCAGAACACT TCAATAACAC ACTGTCTCAG GGACTTCAGT 
AATACAGAGA CCCTGTGCAG CGAGTATAAA TACTACTGTG AGATGTGCTG TAGCAAACAA 
GAGGCACAGA AACGTATGCG TGTGAAGAAG TTGCCTATGA TCTTGGCTCT TCATCTGAAG 
CGCTTTAAGT ACATGGAGCA GCTTCATCGG TACACTAAAC TGAGTTATCG TGTGGTCTTT 
CCACTGGAGC TACGCCTCTT CAACACCTCC GGGGACGCCG TCAATCTCGA CCGCATGTAC 
GACCTCGTCG CTGTGGTGGT CCACTGTGGC AGTGGCCCAA ACAGAGGGCA TTACATCACC 
ATCGTGAAGA GCCATGGCTT CTGGCTGCTG TTTGATGACG ACATTGTGGA GAAAATAGAT 
GCGCAGGCGA TAGAGGAATT CTACGGTTTG ACCTCTGACA TTTCCAAGAA CTCTGAGTCG 
GGATACATTT TGTTTTATCA GTCCAGAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ