cishb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cishb
Entrez Gene ID	100136861
Full Name	cytokine inducible SH2-containing protein b
Synonyms	cish.b,socs8,zgc:175109
General protein information	Preferred Names cytokine inducible SH2-containing protein b Names cytokine inducible SH2-containing protein b suppressor of cytokine signaling 8
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358181.1	XP_017213670.1	cytokine inducible SH2-containing protein b isoform X1
NM_001114554.1	NP_001108026.1	cytokine inducible SH2-containing protein b

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CISH	NP_989957.1
Pan troglodytes (chimpanzee)	CISH	XP_003309858.1
Mus musculus (house mouse)	Cish	NP_034025.1
Canis lupus familiaris (dog)	CISH	XP_541873.3
Rattus norvegicus (Norway rat)	Cish	NP_113992.1
Danio rerio (zebrafish)	cishb	NP_001108026.1
Xenopus tropicalis (tropical clawed frog)	cish	NP_001107161.1
Danio rerio (zebrafish)	cish	NP_001070085.1
Homo sapiens (human)	CISH	NP_659508.1
	CISH	XP_001097824.1
Bos taurus (cattle)	CISH	NP_001040051.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of embryonic hematopoiesis by a cytokine-inducible SH2 domain homolog in zebrafish.
Lewis RS, Noor SM, Fraser FW, Sertori R, Liongue C, Ward AC
Journal of immunology (Baltimore, Md. : 1950)192(12)5739-48(2014 Jun)

Long-term effects of ionizing radiation on gene expression in a zebrafish model.
Jaafar L, Podolsky RH, Dynan WS
PloS one8(7)e69445(2013)

Evolution of JAK-STAT pathway components: mechanisms and role in immune system development.
Liongue C, O'Sullivan LA, Trengove MC, Ward AC
PloS one7(3)e32777(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cishb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cishb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35910	NM_001114554.1 Latest version!	Danio rerio cytokine inducible SH2-containing protein b (cishb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa16933	XM_017358181.1 Latest version!	Danio rerio cytokine inducible SH2-containing protein b (cishb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cishb ( NM_001114554.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001108026.1 Danio rerio cytokine inducible SH2-containing protein b (cishb), mRNA.

Price & Availability↑

CloneID	ODa35910
Clone ID Related Accession (Same CDS sequence)	NM_001114554.1
Accession Version	NM_001114554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-12
Organism	Danio rerio(zebrafish)
Product	cytokine inducible SH2-containing protein b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF195766.1. On or before Jul 18, 2008 this sequence version replaced NM_001114697.1, XM_001334810.1. ##Evidence-Data-START## Transcript exon combination :: EF195766.1, BC155830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505387 [ECO:0000348] ##Evidence-Data-END##

ATGGTTACAA GGGGTACAGG TTCTCAAAAT GAGAGGGGTG AATCTGAAGT TCAACAAACC 
CAGCAGCACC AAGCAGCTCC ATACTCTGAG GACTTTCACT TCATCACCAC CACCTTCCAG 
CACCTGAACA CCTCAGGATG GTACTGGGGT GGTTTGACAG CTGGCGATGC GAGGGCTGCC 
CTAATCGGAG CACCTGAAGG TACTTTCCTC GTGCGGGACA GCAGTCATCC GCTTTACCTC 
TTTACCCTCT CTGTACAGAC CTGGAGAGGC CCTACTAACA TCCGCATAGA GTACGACAGC 
GGCCGATTCC GTCTGGATTC CAGTTTTCCA GCTCGGTCCT GCCTGCTGTC CTTTTCTGCT 
CTTCCTAGTC TCGTGCACCA CTATTCATCC GCACGTCCCG AAGAGGAGAA AAAAGCAGAG 
GACCACCATC ATATGGTGGC AAAGGACACT GGGATACTAC TGAAGCTCAG AAAGCCTCTT 
CACAGGCCTC AAGCCTTTCC CACATTACAG CACCTCACTC GACTCACTAT AAACAGACAC 
ACTGACTGCC ATACAAAACT GCCTCTGCCA CGACCACTGC TGCTTTACTT GCAGGAGTAT 
CCCTTTCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108026.1
CDS	415..1029
Translation	MVTRGTGSQNERGESEVQQTQQHQAAPYSEDFHFITTTFQHLNTSGWYWGGLTAGDARAALIGAPEGTFLVRDSSHPLYLFTLSVQTWRGPTNIRIEYDSGRFRLDSSFPARSCLLSFSALPSLVHHYSSARPEEEKKAEDHHHMVAKDTGILLKLRKPLHRPQAFPTLQHLTRLTINRHTDCHTKLPLPRPLLLYLQEYPFQV

Target ORF information:

RefSeq Version	NM_001114554.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytokine inducible SH2-containing protein b (cishb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114554.1

ORF Insert Sequence:

ATGGTTACAA GGGGTACAGG TTCTCAAAAT GAGAGGGGTG AATCTGAAGT TCAACAAACC 
CAGCAGCACC AAGCAGCTCC ATACTCTGAG GACTTTCACT TCATCACCAC CACCTTCCAG 
CACCTGAACA CCTCAGGATG GTACTGGGGT GGTTTGACAG CTGGCGATGC GAGGGCTGCC 
CTAATCGGAG CACCTGAAGG TACTTTCCTC GTGCGGGACA GCAGTCATCC GCTTTACCTC 
TTTACCCTCT CTGTACAGAC CTGGAGAGGC CCTACTAACA TCCGCATAGA GTACGACAGC 
GGCCGATTCC GTCTGGATTC CAGTTTTCCA GCTCGGTCCT GCCTGCTGTC CTTTTCTGCT 
CTTCCTAGTC TCGTGCACCA CTATTCATCC GCACGTCCCG AAGAGGAGAA AAAAGCAGAG 
GACCACCATC ATATGGTGGC AAAGGACACT GGGATACTAC TGAAGCTCAG AAAGCCTCTT 
CACAGGCCTC AAGCCTTTCC CACATTACAG CACCTCACTC GACTCACTAT AAACAGACAC 
ACTGACTGCC ATACAAAACT GCCTCTGCCA CGACCACTGC TGCTTTACTT GCAGGAGTAT 
CCCTTTCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cishb ( XM_017358181.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213670.1 Danio rerio cytokine inducible SH2-containing protein b (cishb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16933
Clone ID Related Accession (Same CDS sequence)	XM_017358181.1
Accession Version	XM_017358181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cytokine inducible SH2-containing protein b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACAA AGAGGAACCA GTCAGGAATG GTTACAAGGG GTACAGGTTC TCAAAATGAG 
AGGGGTGAAT CTGAAGTTCA ACAAACCCAG CAGCACCAAG CAGCTCCATA CTCTGAGGAC 
TTTCACTTCA TCACCACCAC CTTCCAGCAC CTGAACACCT CAGGATGGTA CTGGGGTGGT 
TTGACAGCTG GCGATGCGAG GGCTGCCCTA ATCGGAGCAC CTGAAGGTAC TTTCCTCGTG 
CGGGACAGCA GTCATCCGCT TTACCTCTTT ACCCTCTCTG TACAGACCTG GAGAGGCCCT 
ACTAACATCC GCATAGAGTA CGACAGCGGC CGATTCCGTC TGGATTCCAG TTTTCCAGCT 
CGGTCCTGCC TGCTGTCCTT TTCTGCTCTT CCTAGTCTCG TGCACCACTA TTCATCCGCA 
CGTCCCGAAG AGGAGAAAAA AGCAGAGGAC CACCATCATA TGGTGGCAAA GGACACTGGG 
ATACTACTGA AGCTCAGAAA GCCTCTTCAC AGGCCTCAAG CCTTTCCCAC ATTACAGCAC 
CTCACTCGAC TCACTATAAA CAGACACACT GACTGCCATA CAAAACTGCC TCTGCCACGA 
CCACTGCTGC TTTACTTGCA GGAGTATCCC TTTCAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213670.1
CDS	396..1037
Translation	MPTKRNQSGMVTRGTGSQNERGESEVQQTQQHQAAPYSEDFHFITTTFQHLNTSGWYWGGLTAGDARAALIGAPEGTFLVRDSSHPLYLFTLSVQTWRGPTNIRIEYDSGRFRLDSSFPARSCLLSFSALPSLVHHYSSARPEEEKKAEDHHHMVAKDTGILLKLRKPLHRPQAFPTLQHLTRLTINRHTDCHTKLPLPRPLLLYLQEYPFQV

Target ORF information:

RefSeq Version	XM_017358181.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytokine inducible SH2-containing protein b (cishb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358181.1

ORF Insert Sequence:

ATGCCCACAA AGAGGAACCA GTCAGGAATG GTTACAAGGG GTACAGGTTC TCAAAATGAG 
AGGGGTGAAT CTGAAGTTCA ACAAACCCAG CAGCACCAAG CAGCTCCATA CTCTGAGGAC 
TTTCACTTCA TCACCACCAC CTTCCAGCAC CTGAACACCT CAGGATGGTA CTGGGGTGGT 
TTGACAGCTG GCGATGCGAG GGCTGCCCTA ATCGGAGCAC CTGAAGGTAC TTTCCTCGTG 
CGGGACAGCA GTCATCCGCT TTACCTCTTT ACCCTCTCTG TACAGACCTG GAGAGGCCCT 
ACTAACATCC GCATAGAGTA CGACAGCGGC CGATTCCGTC TGGATTCCAG TTTTCCAGCT 
CGGTCCTGCC TGCTGTCCTT TTCTGCTCTT CCTAGTCTCG TGCACCACTA TTCATCCGCA 
CGTCCCGAAG AGGAGAAAAA AGCAGAGGAC CACCATCATA TGGTGGCAAA GGACACTGGG 
ATACTACTGA AGCTCAGAAA GCCTCTTCAC AGGCCTCAAG CCTTTCCCAC ATTACAGCAC 
CTCACTCGAC TCACTATAAA CAGACACACT GACTGCCATA CAAAACTGCC TCTGCCACGA 
CCACTGCTGC TTTACTTGCA GGAGTATCCC TTTCAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of embryonic hematopoiesis by a cytokine-inducible SH2 domain homolog in zebrafish.
Journal of immunology (Baltimore, Md. : 1950)192(12)5739-48(2014 Jun)
Lewis RS,Noor SM,Fraser FW,Sertori R,Liongue C,Ward AC

Long-term effects of ionizing radiation on gene expression in a zebrafish model.
PloS one8(7)e69445(2013)
Jaafar L,Podolsky RH,Dynan WS

Evolution of JAK-STAT pathway components: mechanisms and role in immune system development.
PloS one7(3)e32777(2012)
Liongue C,O'Sullivan LA,Trengove MC,Ward AC