vps18 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps18
Entrez Gene ID	100005887
Full Name	vacuolar protein sorting 18 homolog (S. cerevisiae)
Synonyms	chunp6929,hm:zehl0587,wu:fj35h10
General protein information	Preferred Names vacuolar protein sorting 18 homolog (S. cerevisiae) Names vacuolar protein sorting-associated protein 18 homolog vacuolar protein sorting protein 18
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173245.2	NP_775352.2	vacuolar protein sorting-associated protein 18 homolog

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000983	XP_560205.4
Magnaporthe oryzae (rice blast fungus)	MGG_06325	XP_003717259.1
Schizosaccharomyces pombe (fission yeast)	pep3	NP_588498.1
Arabidopsis thaliana (thale cress)	AT1G12470	NP_172709.2
Homo sapiens (human)	VPS18	NP_065908.1
Mus musculus (house mouse)	Vps18	NP_758473.3
Rattus norvegicus (Norway rat)	Vps18	NP_001099969.1
Gallus gallus (chicken)	VPS18	NP_001186584.1
Drosophila melanogaster (fruit fly)	dor	NP_477286.2
Xenopus tropicalis (tropical clawed frog)	vps18	NP_001121454.1
	VPS18	XP_001099146.1
Canis lupus familiaris (dog)	VPS18	XP_544627.3
Oryza sativa (rice)	Os08g0178100	NP_001061126.1
Pan troglodytes (chimpanzee)	VPS18	XP_523187.2
Bos taurus (cattle)	VPS18	NP_001093850.1
Danio rerio (zebrafish)	vps18	NP_775352.2
Neurospora crassa	NCU06183	XP_962995.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish models of human liver development and disease.
Wilkins BJ, Pack M
Comprehensive Physiology3(3)1213-30(2013 Jul)

Genetic characterization and in vivo image analysis of novel zebrafish Danio rerio pigment mutants.
Hsu CC, Pai WY, Lai CY, Lu MW, Her GM
Journal of fish biology82(5)1671-83(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A deficiency of Vps18 in zebrafish larvae results in hepatomegaly and skin hypopigmentation.
Title: The zebrafish mutant vps18 as a model for vesicle-traffic related hypopigmentation diseases.

Mutation of the class C vacuolar protein sorting gene vps18 results in hepatomegaly associated with large, vesicle-filled hepatocytes.
Title: A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver disease.

The following vps18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03530	NM_173245.2 Latest version!	Danio rerio VPS18 core subunit of CORVET and HOPS complexes (vps18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps18 ( NM_173245.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775352.2 Danio rerio VPS18 core subunit of CORVET and HOPS complexes (vps18), mRNA.

Price & Availability↑

CloneID	ODa03530
Clone ID Related Accession (Same CDS sequence)	NM_173245.2
Accession Version	NM_173245.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 18 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086828.1. On or before Dec 3, 2007 this sequence version replaced XM_001342316.1, NM_173245.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086828.1, BC171720.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476811 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCTA TTCTGGATCA ATATGAAGAT TCGCAGAACA TCCGTCAACA CAGTCGTATG 
TCCACGGCCA ACATCGGCAT CACTCATTCA GGGTTTGTAA ATGTAAGATT GGAGGAAGAA 
AAACCCATTT TCACCAAGCA GCGCATCGAT TTCTCTCCTC CCGAAAAAAT CAACCAATTT 
TCGGTTTGCA ACAATCAGTT ATGCATGAGC TTGGGGAAAG ACACCCTGCT GAGAATTGAT 
CTCGGAAAAC CTGATCAGCC AAATCAGATT GAATTGGGAA GAAAAGATGA TAGCAAAGTA 
CACAGACTTT TCCTAGATCC TACAGGGTCA CATTTAGTCA TCTGCCTCAC TACAAATGAA 
TGTGTGTACC TAAATAGAAA CACTCAGAAG GTCCGAGGTC TGTCAAGGTG GAGAGGCCAC 
TTGATTGAAA GCATAGGTTG GAACAAATTA ATCGGCTCTG AGACCAACAC TGGCCCCATT 
CTGGTCGGGA CCAGCCAGGG AATCATCTTT GAAGCTGAGA TCTCTGCCTC AGAGGGCAGT 
CTGTTCAACA CCAACCCGGA CCAGTACTTC AGACAGGTTC ATTACCTCGA GGAGGATGGC 
AAGCCAGCTC CGGTTTGTTG CCTGGAGGTT GAACGTGGTT TGGAAACTAA ATACTTTATC 
ATTGCAACCA CACGAAAACG GCTGTTCCAG TTTGTAGGAA AGCTGGCAGA AGGATCTGAG 
CAGCAGGGCT TCAGCTCCAT CTTTGCCCAG AACCAGGATC TTCTACCCAG CTTCCAGGAA 
TTTCCTGTCA ATATGGGCTA TAGTGAGATT ACATTTTACA CATCAAAGCT GCGTAGTCGC 
CCCAAGACCT TCGCCTGGAT GATGGGGAAT GGCGTTTTGT ACGGGCAACT GGACTATGTG 
CGCCCCGACT CTTTACTCAG TGATGTTCAA GTGTGGGAAT ACACGCAAGA CATTGATTTA 
AACTTCGTCA AACCCATCTC TATAGTCCTC ACCCAGTTCC ACTTCTTGCT CCTGCTCCCT 
GACCGTGTCC GAGGGATCTG CACCCTGAAT GGGCAGGTTG TGCATGAAGA TGTGTTTCCC 
GAAAAGTTCG GTACACTACA AAAGATGATC AAAGATCCCA TAACAGGACT TGTGTGGATC 
TACACTGAGA AAGCAGTGTT TCGATATCAC ATCCAGAAAG AAGCACGGGA CGTTTGGCAG 
ATGTACATGA ACATGAATAA GTTTGATCTG GCCAAAGAGT ACTGCAAAGA TCGACCAGAA 
TGCTTAGACA TGGTTCTCGC AAAAGAGGCA GAGCACTGCT TTCAAAACAA GCGCTACCTA 
GAGAGCGCCA AATGCTATGC GCTTACTCAA AATTACTTTG AGGAGATTGC GCTGAAGTTC 
ATTGAGGCCA AGCAAGAGGA GGCCTTGAAA GAGTTTCTGA TTAAAAAGCT GGTCAATCTC 
AAGCCCAGTG AGAAGACTCA GATCACCCTG CTGGTGACCT GGTTGACCGA ACTCTATCTG 
AATCGGCTCG GTCAACTGGA GGCCGACGAA GGCAAGCAAC ATTTATTTCT GGAGACCCGT 
GAAGAGTTTC GTACCTTTCT TAAAAGCCCC AAACATAAAG ATTGCTTCTA CAACAATCGC 
AGCACCATCT ACGACCTGCT CGCTAGTCAT GGCGACGTGG ACAACATGGT GTATTTCTCG 
GTCATCATGC AAGATTACGA GCGTGTCATT TCTCATTACT GCCAGCATGA CGACTACAGC 
GCAGCTTTAG ATGTTCTCTC CAAACACTGT GACGACAAGC TCTTCTACAA GTTCTCCCCA 
GTCCTCATGC AGCACATCCC CAAAAAGGTG GTCGATGCAT GGATTCAGAT GGGAAACCGG 
TTGGATCCGA AGAATCTGAT CCCAGCGCTT GTGAACTACA GCCAGATGGG CAGCATGCAG 
CAGATAAATG AGACCATCCG GTACATGGAG TTCTGTGTGT ATGAGCTGGA CGTCAAAGAA 
GAGGCCATTC ACAATTACCT ACTCTCGCTT TATGCCAAAC ACAAGCCAGA CGCTCTGTTG 
TGGTACCTCG AACAAGCCGG AACTCACGTC TCCGATATTC ATTATGACTT GAAATATGCC 
TTGCGCCTTT GCTCCGAACA TGGATACCTG CAGGCCTGCG TCTTGGTTTA CAAGATTATG 
GAGCTGTATG AGGAAGCTGT GGATCTTGCT CTAAAAGTGG ATGTGGATTT GGCCAAGTCT 
TGCGCCGACC TTCCAGAGGA CGATGAGGAG TTAAGAAAGA AGCTGTGGCT GAAGATCGCT 
CGCCATGTAG TGCAGGAGGA GAAGGATGTC AAGAAGGCTA TGAACTGCCT GTCCAGTTGC 
AACCTGCTTA AGATCGAAGA CATCTTGCCT TTCTTTCCAG ACTTTGTCAC AATAGACCAC 
TTCAAGGAGG CGATATGCAG CTCTTTGGAA GAGTACAACA AGCACATCGA AGAGCTGAAA 
CAAGAAATGG AGGAGGCCAC AGAAAGTGCC AAACGCATCC GCGAAGACAT CCAAGAGATG 
AGGAACAAGT ATGGTGTGGT TGAGTCTCAG GAAAAATGCG CTACTTGTGA TTTCCCCCTG 
CTCAACCGAC CTTTCTATCT TTTCCTGTGC GGACACATGT TCCATTATGA TTGCCTCCTG 
CAGGAAGTCA TTCCTCACCT CTCTGTCTAT AAGCAGAATA AATTGGATGA GCTACAGAAG 
AAACTGGCAG CGACCACGCA GACCACCAAG GCTCGGCACA AGCCTAGAGA GGAGGACACG 
GTCAGCTTAG GCAAAGGCCA GGGAAGCAGG GAGCAGATCA AATCAGACAT TGATGATATT 
ATAGCGTGCG AGTGCGTGTA CTGCGGTGAG CTGATGATCA AGTCCATTGA CAAGCCGTTC 
ATAGATCCGC AGAAGTTTGA TCAAGAGATG TCCAGCTGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775352.2
CDS	311..3235
Translation	MASILDQYEDSQNIRQHSRMSTANIGITHSGFVNVRLEEEKPIFTKQRIDFSPPEKINQFSVCNNQLCMSLGKDTLLRIDLGKPDQPNQIELGRKDDSKVHRLFLDPTGSHLVICLTTNECVYLNRNTQKVRGLSRWRGHLIESIGWNKLIGSETNTGPILVGTSQGIIFEAEISASEGSLFNTNPDQYFRQVHYLEEDGKPAPVCCLEVERGLETKYFIIATTRKRLFQFVGKLAEGSEQQGFSSIFAQNQDLLPSFQEFPVNMGYSEITFYTSKLRSRPKTFAWMMGNGVLYGQLDYVRPDSLLSDVQVWEYTQDIDLNFVKPISIVLTQFHFLLLLPDRVRGICTLNGQVVHEDVFPEKFGTLQKMIKDPITGLVWIYTEKAVFRYHIQKEARDVWQMYMNMNKFDLAKEYCKDRPECLDMVLAKEAEHCFQNKRYLESAKCYALTQNYFEEIALKFIEAKQEEALKEFLIKKLVNLKPSEKTQITLLVTWLTELYLNRLGQLEADEGKQHLFLETREEFRTFLKSPKHKDCFYNNRSTIYDLLASHGDVDNMVYFSVIMQDYERVISHYCQHDDYSAALDVLSKHCDDKLFYKFSPVLMQHIPKKVVDAWIQMGNRLDPKNLIPALVNYSQMGSMQQINETIRYMEFCVYELDVKEEAIHNYLLSLYAKHKPDALLWYLEQAGTHVSDIHYDLKYALRLCSEHGYLQACVLVYKIMELYEEAVDLALKVDVDLAKSCADLPEDDEELRKKLWLKIARHVVQEEKDVKKAMNCLSSCNLLKIEDILPFFPDFVTIDHFKEAICSSLEEYNKHIEELKQEMEEATESAKRIREDIQEMRNKYGVVESQEKCATCDFPLLNRPFYLFLCGHMFHYDCLLQEVIPHLSVYKQNKLDELQKKLAATTQTTKARHKPREEDTVSLGKGQGSREQIKSDIDDIIACECVYCGELMIKSIDKPFIDPQKFDQEMSSWL

Target ORF information:

RefSeq Version	NM_173245.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio VPS18 core subunit of CORVET and HOPS complexes (vps18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173245.2

ORF Insert Sequence:

ATGGCATCTA TTCTGGATCA ATATGAAGAT TCGCAGAACA TCCGTCAACA CAGTCGTATG 
TCCACGGCCA ACATCGGCAT CACTCATTCA GGGTTTGTAA ATGTAAGATT GGAGGAAGAA 
AAACCCATTT TCACCAAGCA GCGCATCGAT TTCTCTCCTC CCGAAAAAAT CAACCAATTT 
TCGGTTTGCA ACAATCAGTT ATGCATGAGC TTGGGGAAAG ACACCCTGCT GAGAATTGAT 
CTCGGAAAAC CTGATCAGCC AAATCAGATT GAATTGGGAA GAAAAGATGA TAGCAAAGTA 
CACAGACTTT TCCTAGATCC TACAGGGTCA CATTTAGTCA TCTGCCTCAC TACAAATGAA 
TGTGTGTACC TAAATAGAAA CACTCAGAAG GTCCGAGGTC TGTCAAGGTG GAGAGGCCAC 
TTGATTGAAA GCATAGGTTG GAACAAATTA ATCGGCTCTG AGACCAACAC TGGCCCCATT 
CTGGTCGGGA CCAGCCAGGG AATCATCTTT GAAGCTGAGA TCTCTGCCTC AGAGGGCAGT 
CTGTTCAACA CCAACCCGGA CCAGTACTTC AGACAGGTTC ATTACCTCGA GGAGGATGGC 
AAGCCAGCTC CGGTTTGTTG CCTGGAGGTT GAACGTGGTT TGGAAACTAA ATACTTTATC 
ATTGCAACCA CACGAAAACG GCTGTTCCAG TTTGTAGGAA AGCTGGCAGA AGGATCTGAG 
CAGCAGGGCT TCAGCTCCAT CTTTGCCCAG AACCAGGATC TTCTACCCAG CTTCCAGGAA 
TTTCCTGTCA ATATGGGCTA TAGTGAGATT ACATTTTACA CATCAAAGCT GCGTAGTCGC 
CCCAAGACCT TCGCCTGGAT GATGGGGAAT GGCGTTTTGT ACGGGCAACT GGACTATGTG 
CGCCCCGACT CTTTACTCAG TGATGTTCAA GTGTGGGAAT ACACGCAAGA CATTGATTTA 
AACTTCGTCA AACCCATCTC TATAGTCCTC ACCCAGTTCC ACTTCTTGCT CCTGCTCCCT 
GACCGTGTCC GAGGGATCTG CACCCTGAAT GGGCAGGTTG TGCATGAAGA TGTGTTTCCC 
GAAAAGTTCG GTACACTACA AAAGATGATC AAAGATCCCA TAACAGGACT TGTGTGGATC 
TACACTGAGA AAGCAGTGTT TCGATATCAC ATCCAGAAAG AAGCACGGGA CGTTTGGCAG 
ATGTACATGA ACATGAATAA GTTTGATCTG GCCAAAGAGT ACTGCAAAGA TCGACCAGAA 
TGCTTAGACA TGGTTCTCGC AAAAGAGGCA GAGCACTGCT TTCAAAACAA GCGCTACCTA 
GAGAGCGCCA AATGCTATGC GCTTACTCAA AATTACTTTG AGGAGATTGC GCTGAAGTTC 
ATTGAGGCCA AGCAAGAGGA GGCCTTGAAA GAGTTTCTGA TTAAAAAGCT GGTCAATCTC 
AAGCCCAGTG AGAAGACTCA GATCACCCTG CTGGTGACCT GGTTGACCGA ACTCTATCTG 
AATCGGCTCG GTCAACTGGA GGCCGACGAA GGCAAGCAAC ATTTATTTCT GGAGACCCGT 
GAAGAGTTTC GTACCTTTCT TAAAAGCCCC AAACATAAAG ATTGCTTCTA CAACAATCGC 
AGCACCATCT ACGACCTGCT CGCTAGTCAT GGCGACGTGG ACAACATGGT GTATTTCTCG 
GTCATCATGC AAGATTACGA GCGTGTCATT TCTCATTACT GCCAGCATGA CGACTACAGC 
GCAGCTTTAG ATGTTCTCTC CAAACACTGT GACGACAAGC TCTTCTACAA GTTCTCCCCA 
GTCCTCATGC AGCACATCCC CAAAAAGGTG GTCGATGCAT GGATTCAGAT GGGAAACCGG 
TTGGATCCGA AGAATCTGAT CCCAGCGCTT GTGAACTACA GCCAGATGGG CAGCATGCAG 
CAGATAAATG AGACCATCCG GTACATGGAG TTCTGTGTGT ATGAGCTGGA CGTCAAAGAA 
GAGGCCATTC ACAATTACCT ACTCTCGCTT TATGCCAAAC ACAAGCCAGA CGCTCTGTTG 
TGGTACCTCG AACAAGCCGG AACTCACGTC TCCGATATTC ATTATGACTT GAAATATGCC 
TTGCGCCTTT GCTCCGAACA TGGATACCTG CAGGCCTGCG TCTTGGTTTA CAAGATTATG 
GAGCTGTATG AGGAAGCTGT GGATCTTGCT CTAAAAGTGG ATGTGGATTT GGCCAAGTCT 
TGCGCCGACC TTCCAGAGGA CGATGAGGAG TTAAGAAAGA AGCTGTGGCT GAAGATCGCT 
CGCCATGTAG TGCAGGAGGA GAAGGATGTC AAGAAGGCTA TGAACTGCCT GTCCAGTTGC 
AACCTGCTTA AGATCGAAGA CATCTTGCCT TTCTTTCCAG ACTTTGTCAC AATAGACCAC 
TTCAAGGAGG CGATATGCAG CTCTTTGGAA GAGTACAACA AGCACATCGA AGAGCTGAAA 
CAAGAAATGG AGGAGGCCAC AGAAAGTGCC AAACGCATCC GCGAAGACAT CCAAGAGATG 
AGGAACAAGT ATGGTGTGGT TGAGTCTCAG GAAAAATGCG CTACTTGTGA TTTCCCCCTG 
CTCAACCGAC CTTTCTATCT TTTCCTGTGC GGACACATGT TCCATTATGA TTGCCTCCTG 
CAGGAAGTCA TTCCTCACCT CTCTGTCTAT AAGCAGAATA AATTGGATGA GCTACAGAAG 
AAACTGGCAG CGACCACGCA GACCACCAAG GCTCGGCACA AGCCTAGAGA GGAGGACACG 
GTCAGCTTAG GCAAAGGCCA GGGAAGCAGG GAGCAGATCA AATCAGACAT TGATGATATT 
ATAGCGTGCG AGTGCGTGTA CTGCGGTGAG CTGATGATCA AGTCCATTGA CAAGCCGTTC 
ATAGATCCGC AGAAGTTTGA TCAAGAGATG TCCAGCTGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish models of human liver development and disease.
Comprehensive Physiology3(3)1213-30(2013 Jul)
Wilkins BJ,Pack M

Genetic characterization and in vivo image analysis of novel zebrafish Danio rerio pigment mutants.
Journal of fish biology82(5)1671-83(2013 May)
Hsu CC,Pai WY,Lai CY,Lu MW,Her GM