bmp2k cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene
Clones
gRNAs

Summary

Gene Symbol	bmp2k
Entrez Gene ID	107083991
Full Name	BMP2 inducible kinase
Gene Type	protein-coding
Organism	Cyprinodon variegatus(sheepshead minnow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015373613.1	XP_015229099.1	BMP-2-inducible protein kinase isoform X1
XM_015373614.1	XP_015229100.1	BMP-2-inducible protein kinase isoform X2

Pathways from BioSystems
Gene Ontology

The following bmp2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy06599	XM_015373613.1 Latest version!	Cyprinodon variegatus BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCy06600	XM_015373614.1 Latest version!	Cyprinodon variegatus BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp2k ( XM_015373613.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015229099.1 Cyprinodon variegatus BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCy06599
Clone ID Related Accession (Same CDS sequence)	XM_015373613.1
Accession Version	XM_015373613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	BMP-2-inducible protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGAAT GCAAAAGTCC GAGAGCGGAG GACTCGGAGG GCCGTCCGGT 
TCCAGCTCCA GCTCGGGGGT CAGCAGCTAC TTCGGGAAAG TGTTCGCAGT GGGCCGGTAT 
CAGGTCACCG TGGAGGAGCT CGTCGCTGAA GGGGGCTTTT CTGTGGTGTT CCTGGCTCGG 
ACGCATAGCG GCGTCCGCTG CGCTCTTAAA AGAATGTACG TGAACAACGT GCCGGACCTG 
AATGTCTACA AGAGAGAGAT CACCATCATG AAAGAGCTGT CGGGCCACAA GAACATTGTC 
CGTTACCTGG ACTCGACAAT CAGCTCGGTT TCAGACAGTG TGTGGGAGGT CCTGATTCTC 
ATGGAGTACT GCAAAGCGGG TCAGGTAGTG AAGCAGATGA ACCAGCGGTT AAATGTGGGT 
TTCAGTGAAG CCGAGGTCCT CCACATCTTC TGTGACACAT GTGAAGCTGT GGCTCGCCTT 
CATCAATGCA AGACCCCCAT CATTCACAGG GACCTTAAGG TTGAAAACAT CCTTCTGAAC 
GACCAGGGAA ACTACGTCCT GTGTGACTTC GGCAGCTCCA CTCATAAGAT CCTGTTGCCT 
CTTAAAGACG GAGTGACGGC CGTGGAGGAT GAGATAAAGA AGTACACAAC CCTCTCATAT 
CGGGCCCCGG AGATGATTAA CTTGTACGCA GGAAAAGCCA TCACCACTAA AGCTGATATC 
TGGGCACTCG GATGCTTGTT GTACAAGCTG TGTTTCTTCA CACTTCCATT TGGGGAGAGT 
CAGGTGGCCA TATGTGACGG ATCCTTTATC GTCCCGGATA ACTCAAAGTT CTCCACCAAG 
TTGCACTGCT TGATCAGATA TATGCTTGAG CCGGACCAGG AGAAAAGACC AGACATCTAC 
CAAGTGTCCT ACTTTGCCTT TAAATTAGCT GGAAGAGACT GCCCAGTCCC AAACCTCTCA 
AACTCTCCCA TCCCAACATC TCTTCCTGAG CCTCTAACAG CCAGTGAGGT GGCTGCTAAG 
AAGAGTATGA CTAAAGCCAG GATAACCGAC TCCGTGGGTC CAACAGAAAC ATCCATAGCT 
CCCAGGCAGA GGCCGAAGGC TGCAAACAGC AACGTCCTGC CTCTCCCCAA CACAGTCACT 
CCTGTCAAAA TGACCGTGCC TTCAGCACCC GTCAGCAACG GCCAGAAAGC TCCCACTCCT 
GGATCGGGGC AGCCGACTGT GCAGACGGCG CCCAGCAGCC AGCAGCACCG CGTCCTGCAG 
CAGCTGCAGC CTGGGGACCT GCGTCTGCAG CAGCTTCAAC AACAACAAGC AGTGCAGCAG 
CAACATGCTA ATGCTCAGCA TCTGCAATAC TTGCAGTACC AACAGGCAGT GCAGCAGTCC 
CTGCAGCTTC AGCACCAGCA GGCTCTGCTC CAGCAGCAGC AGCAGCAGAT GATGATGCAG 
CCCATGTATC AGCAGCAAGT CGCTCAGGCC CAATATGCTG CCTTGCTGCA CCAGTACCAA 
CAGGCTTTTG TCCAGCAGCA GCAGCACCAC CATCATCATC ATCAGCAGCA GCAGCTTCCC 
GCTCAGCAGC CTCTTCCCTA TATGTCTTCC CCTCTTGAGT TCCAGATCCC TCTGGCTTCT 
CTAAACGCCA CCACGCCCAC TGCTGGATCC GGAGCTGCAC TTGGACCTGG ATCTGGACCA 
TCCCCTGTTG AAACCTCATA CACTAACCCC AGCAGTAGAA ACGCCCTGCT GGCCACAGAT 
GGTATCACCC CAACCTCTCA GAGCAGCAGC GCCGTCAGTC ACCCCCCCGA CATGTCGGGC 
TGGAACCCTT TTGGAGAGGA CAACTTCTCC AAACTAACGG AGGAGGAGCT GATCGACAGA 
GAGTTCGACC TGCTCAGAGC AAAGAAGCCA GAGGAGAGGA CAGCCGGCGT GGAAACGGAG 
CCATCGCCAT CTACAGCTCT TCCACCGGAG GATCTGTTCG GCTCAGTCCC ATTCGTGGCC 
AACGCAGGAC CTGCAGTGAT GAGCAGTGAG CAGCCTGTGG AGGTCGTCTC CAACATCCCA 
GCTCCCATCA AGGACCCGCA CTCAGTCAAG GAGTCCAAAC CAGGAAAGAA GAACAGCTGT 
AAACCTGCAG ATGAGTCCGA CAGCGACTTC GAGTCTGACC CGCCTTCGCC TAAGAGCAGT 
GAGGATGAAG AGCAGGAGGA AGAGGAGGCC TTGAACAGTG AACACGGTGA AGACACAGAG 
CCAGAGAACC TTGGACAGAG GCCTCTGCTG ATGGACTCTG AGGAAGAGGA GGACGAGGAC 
AAACACAGCT CAGATTCAGA CTGTGATGCC AACAAGGTTA AAATCCGCTC AAAGAAACCT 
GCAGCCGCTA AAGTAGCCGA TCCCAGTGCT TCCAGGAGTC CACTAGGGGG GTCTAGGACC 
AGTCTGGTCA CCCCTCCAGT GTCGCCCAGT GGAGCCAGAA ACACTGACGT TTTTGGTGCC 
ATCCCTTTTG TCAGAGGAGC TTCACCAGCG AGCCCCTCAG AGATCACTGA TATCTTCACT 
AAAGCACCTT TCAGGCAGGT CAGTCAGGAA GACCCGGCCG CCGACGATTT TGATGTTTTT 
ACCAAAGCCC CGTTCAGTCG GAATGTTTCC AAGTCGGGAA GGAGCGGCAG CGATGTCACC 
ATGGGACAAA CGCCGCCGGT ATCTCCCGAA GGTACCGACG TCTTCGGCTT CTCGCCTTTT 
CAGCCGGGCC CCTCAGAGCC TCCTCCCACC ACCTCCAGGA GCGCAGAGGA CATCTTCGAG 
TTGCCATTTG AGGATTCGGC AAACCTGCAG AGGCAGAAGC AACGCAGTCT GCAGAAGCTG 
TCATCACGCC AGAGAAAAAC CAAGCAGGAG GCGACGGCGG GGGGTGGCAA CGGCAAGCGG 
CATCACAGCA CCCCGACCGG AGGACGGAAG GGCAACAAGC CGACCTACCG CACCCCAGAG 
AGGGTCCGCA GGCACAAGAA GGTGGGCCGC AGAGATTCTC AGAGCAGCAA CGAGTTCCTG 
AGTACCTCCG ACTCCAAGGA GAACATCAGC GTGGACATCA GCATGGCTGA AGGGAAAGAC 
AAAGCAGCGC CTCTGCCGGT GGACGATGCT CTGTTGGACC TCTTTGGGGC CAAACCGTTC 
CACCCTCCAG ACAGCAGCCG CCTCGGCCAG TACCCCCAGC TCAGCGACGC CAGGGGTGAC 
ATGAGCACCG CTAACGGGAA GTCATGGAGT GGCGGCGAAA GCAGGAGCAT GGATGATTTC 
GGGGCGGTGC CCTTCACAGA ACTGGTGATT CACAGTGGAC CGCAGCAGCC CGCGGCGCCT 
TCGCAGCCGC TGGACCTCGA CCCTTTTGGG GCGGCGCCGT TTCCCTCCAA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015229099.1
CDS	631..3987
Translation	MKKFSRMQKSESGGLGGPSGSSSSSGVSSYFGKVFAVGRYQVTVEELVAEGGFSVVFLARTHSGVRCALKRMYVNNVPDLNVYKREITIMKELSGHKNIVRYLDSTISSVSDSVWEVLILMEYCKAGQVVKQMNQRLNVGFSEAEVLHIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDQGNYVLCDFGSSTHKILLPLKDGVTAVEDEIKKYTTLSYRAPEMINLYAGKAITTKADIWALGCLLYKLCFFTLPFGESQVAICDGSFIVPDNSKFSTKLHCLIRYMLEPDQEKRPDIYQVSYFAFKLAGRDCPVPNLSNSPIPTSLPEPLTASEVAAKKSMTKARITDSVGPTETSIAPRQRPKAANSNVLPLPNTVTPVKMTVPSAPVSNGQKAPTPGSGQPTVQTAPSSQQHRVLQQLQPGDLRLQQLQQQQAVQQQHANAQHLQYLQYQQAVQQSLQLQHQQALLQQQQQQMMMQPMYQQQVAQAQYAALLHQYQQAFVQQQQHHHHHHQQQQLPAQQPLPYMSSPLEFQIPLASLNATTPTAGSGAALGPGSGPSPVETSYTNPSSRNALLATDGITPTSQSSSAVSHPPDMSGWNPFGEDNFSKLTEEELIDREFDLLRAKKPEERTAGVETEPSPSTALPPEDLFGSVPFVANAGPAVMSSEQPVEVVSNIPAPIKDPHSVKESKPGKKNSCKPADESDSDFESDPPSPKSSEDEEQEEEEALNSEHGEDTEPENLGQRPLLMDSEEEEDEDKHSSDSDCDANKVKIRSKKPAAAKVADPSASRSPLGGSRTSLVTPPVSPSGARNTDVFGAIPFVRGASPASPSEITDIFTKAPFRQVSQEDPAADDFDVFTKAPFSRNVSKSGRSGSDVTMGQTPPVSPEGTDVFGFSPFQPGPSEPPPTTSRSAEDIFELPFEDSANLQRQKQRSLQKLSSRQRKTKQEATAGGGNGKRHHSTPTGGRKGNKPTYRTPERVRRHKKVGRRDSQSSNEFLSTSDSKENISVDISMAEGKDKAAPLPVDDALLDLFGAKPFHPPDSSRLGQYPQLSDARGDMSTANGKSWSGGESRSMDDFGAVPFTELVIHSGPQQPAAPSQPLDLDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_015373613.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015373613.1

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGAAT GCAAAAGTCC GAGAGCGGAG GACTCGGAGG GCCGTCCGGT 
TCCAGCTCCA GCTCGGGGGT CAGCAGCTAC TTCGGGAAAG TGTTCGCAGT GGGCCGGTAT 
CAGGTCACCG TGGAGGAGCT CGTCGCTGAA GGGGGCTTTT CTGTGGTGTT CCTGGCTCGG 
ACGCATAGCG GCGTCCGCTG CGCTCTTAAA AGAATGTACG TGAACAACGT GCCGGACCTG 
AATGTCTACA AGAGAGAGAT CACCATCATG AAAGAGCTGT CGGGCCACAA GAACATTGTC 
CGTTACCTGG ACTCGACAAT CAGCTCGGTT TCAGACAGTG TGTGGGAGGT CCTGATTCTC 
ATGGAGTACT GCAAAGCGGG TCAGGTAGTG AAGCAGATGA ACCAGCGGTT AAATGTGGGT 
TTCAGTGAAG CCGAGGTCCT CCACATCTTC TGTGACACAT GTGAAGCTGT GGCTCGCCTT 
CATCAATGCA AGACCCCCAT CATTCACAGG GACCTTAAGG TTGAAAACAT CCTTCTGAAC 
GACCAGGGAA ACTACGTCCT GTGTGACTTC GGCAGCTCCA CTCATAAGAT CCTGTTGCCT 
CTTAAAGACG GAGTGACGGC CGTGGAGGAT GAGATAAAGA AGTACACAAC CCTCTCATAT 
CGGGCCCCGG AGATGATTAA CTTGTACGCA GGAAAAGCCA TCACCACTAA AGCTGATATC 
TGGGCACTCG GATGCTTGTT GTACAAGCTG TGTTTCTTCA CACTTCCATT TGGGGAGAGT 
CAGGTGGCCA TATGTGACGG ATCCTTTATC GTCCCGGATA ACTCAAAGTT CTCCACCAAG 
TTGCACTGCT TGATCAGATA TATGCTTGAG CCGGACCAGG AGAAAAGACC AGACATCTAC 
CAAGTGTCCT ACTTTGCCTT TAAATTAGCT GGAAGAGACT GCCCAGTCCC AAACCTCTCA 
AACTCTCCCA TCCCAACATC TCTTCCTGAG CCTCTAACAG CCAGTGAGGT GGCTGCTAAG 
AAGAGTATGA CTAAAGCCAG GATAACCGAC TCCGTGGGTC CAACAGAAAC ATCCATAGCT 
CCCAGGCAGA GGCCGAAGGC TGCAAACAGC AACGTCCTGC CTCTCCCCAA CACAGTCACT 
CCTGTCAAAA TGACCGTGCC TTCAGCACCC GTCAGCAACG GCCAGAAAGC TCCCACTCCT 
GGATCGGGGC AGCCGACTGT GCAGACGGCG CCCAGCAGCC AGCAGCACCG CGTCCTGCAG 
CAGCTGCAGC CTGGGGACCT GCGTCTGCAG CAGCTTCAAC AACAACAAGC AGTGCAGCAG 
CAACATGCTA ATGCTCAGCA TCTGCAATAC TTGCAGTACC AACAGGCAGT GCAGCAGTCC 
CTGCAGCTTC AGCACCAGCA GGCTCTGCTC CAGCAGCAGC AGCAGCAGAT GATGATGCAG 
CCCATGTATC AGCAGCAAGT CGCTCAGGCC CAATATGCTG CCTTGCTGCA CCAGTACCAA 
CAGGCTTTTG TCCAGCAGCA GCAGCACCAC CATCATCATC ATCAGCAGCA GCAGCTTCCC 
GCTCAGCAGC CTCTTCCCTA TATGTCTTCC CCTCTTGAGT TCCAGATCCC TCTGGCTTCT 
CTAAACGCCA CCACGCCCAC TGCTGGATCC GGAGCTGCAC TTGGACCTGG ATCTGGACCA 
TCCCCTGTTG AAACCTCATA CACTAACCCC AGCAGTAGAA ACGCCCTGCT GGCCACAGAT 
GGTATCACCC CAACCTCTCA GAGCAGCAGC GCCGTCAGTC ACCCCCCCGA CATGTCGGGC 
TGGAACCCTT TTGGAGAGGA CAACTTCTCC AAACTAACGG AGGAGGAGCT GATCGACAGA 
GAGTTCGACC TGCTCAGAGC AAAGAAGCCA GAGGAGAGGA CAGCCGGCGT GGAAACGGAG 
CCATCGCCAT CTACAGCTCT TCCACCGGAG GATCTGTTCG GCTCAGTCCC ATTCGTGGCC 
AACGCAGGAC CTGCAGTGAT GAGCAGTGAG CAGCCTGTGG AGGTCGTCTC CAACATCCCA 
GCTCCCATCA AGGACCCGCA CTCAGTCAAG GAGTCCAAAC CAGGAAAGAA GAACAGCTGT 
AAACCTGCAG ATGAGTCCGA CAGCGACTTC GAGTCTGACC CGCCTTCGCC TAAGAGCAGT 
GAGGATGAAG AGCAGGAGGA AGAGGAGGCC TTGAACAGTG AACACGGTGA AGACACAGAG 
CCAGAGAACC TTGGACAGAG GCCTCTGCTG ATGGACTCTG AGGAAGAGGA GGACGAGGAC 
AAACACAGCT CAGATTCAGA CTGTGATGCC AACAAGGTTA AAATCCGCTC AAAGAAACCT 
GCAGCCGCTA AAGTAGCCGA TCCCAGTGCT TCCAGGAGTC CACTAGGGGG GTCTAGGACC 
AGTCTGGTCA CCCCTCCAGT GTCGCCCAGT GGAGCCAGAA ACACTGACGT TTTTGGTGCC 
ATCCCTTTTG TCAGAGGAGC TTCACCAGCG AGCCCCTCAG AGATCACTGA TATCTTCACT 
AAAGCACCTT TCAGGCAGGT CAGTCAGGAA GACCCGGCCG CCGACGATTT TGATGTTTTT 
ACCAAAGCCC CGTTCAGTCG GAATGTTTCC AAGTCGGGAA GGAGCGGCAG CGATGTCACC 
ATGGGACAAA CGCCGCCGGT ATCTCCCGAA GGTACCGACG TCTTCGGCTT CTCGCCTTTT 
CAGCCGGGCC CCTCAGAGCC TCCTCCCACC ACCTCCAGGA GCGCAGAGGA CATCTTCGAG 
TTGCCATTTG AGGATTCGGC AAACCTGCAG AGGCAGAAGC AACGCAGTCT GCAGAAGCTG 
TCATCACGCC AGAGAAAAAC CAAGCAGGAG GCGACGGCGG GGGGTGGCAA CGGCAAGCGG 
CATCACAGCA CCCCGACCGG AGGACGGAAG GGCAACAAGC CGACCTACCG CACCCCAGAG 
AGGGTCCGCA GGCACAAGAA GGTGGGCCGC AGAGATTCTC AGAGCAGCAA CGAGTTCCTG 
AGTACCTCCG ACTCCAAGGA GAACATCAGC GTGGACATCA GCATGGCTGA AGGGAAAGAC 
AAAGCAGCGC CTCTGCCGGT GGACGATGCT CTGTTGGACC TCTTTGGGGC CAAACCGTTC 
CACCCTCCAG ACAGCAGCCG CCTCGGCCAG TACCCCCAGC TCAGCGACGC CAGGGGTGAC 
ATGAGCACCG CTAACGGGAA GTCATGGAGT GGCGGCGAAA GCAGGAGCAT GGATGATTTC 
GGGGCGGTGC CCTTCACAGA ACTGGTGATT CACAGTGGAC CGCAGCAGCC CGCGGCGCCT 
TCGCAGCCGC TGGACCTCGA CCCTTTTGGG GCGGCGCCGT TTCCCTCCAA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bmp2k ( XM_015373614.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015229100.1 Cyprinodon variegatus BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCy06600
Clone ID Related Accession (Same CDS sequence)	XM_015373614.1
Accession Version	XM_015373614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	BMP-2-inducible protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCCAGAAT GCAAAAGTCC GAGAGCGGAG GACTCGGAGG GCCGTCCGGT 
TCCAGCTCCA GCTCGGGGGT CAGCAGCTAC TTCGGGAAAG TGTTCGCAGT GGGCCGGTAT 
CAGGTCACCG TGGAGGAGCT CGTCGCTGAA GGGGGCTTTT CTGTGGTGTT CCTGGCTCGG 
ACGCATAGCG GCGTCCGCTG CGCTCTTAAA AGAATGTACG TGAACAACGT GCCGGACCTG 
AATGTCTACA AGAGAGAGAT CACCATCATG AAAGAGCTGT CGGGCCACAA GAACATTGTC 
CGTTACCTGG ACTCGACAAT CAGCTCGGTT TCAGACAGTG TGTGGGAGGT CCTGATTCTC 
ATGGAGTACT GCAAAGCGGG TCAGGTAGTG AAGCAGATGA ACCAGCGGTT AAATGTGGGT 
TTCAGTGAAG CCGAGGTCCT CCACATCTTC TGTGACACAT GTGAAGCTGT GGCTCGCCTT 
CATCAATGCA AGACCCCCAT CATTCACAGG GACCTTAAGG TTGAAAACAT CCTTCTGAAC 
GACCAGGGAA ACTACGTCCT GTGTGACTTC GGCAGCTCCA CTCATAAGAT CCTGTTGCCT 
CTTAAAGACG GAGTGACGGC CGTGGAGGAT GAGATAAAGA AGTACACAAC CCTCTCATAT 
CGGGCCCCGG AGATGATTAA CTTGTACGCA GGAAAAGCCA TCACCACTAA AGCTGATATC 
TGGGCACTCG GATGCTTGTT GTACAAGCTG TGTTTCTTCA CACTTCCATT TGGGGAGAGT 
CAGGTGGCCA TATGTGACGG ATCCTTTATC GTCCCGGATA ACTCAAAGTT CTCCACCAAG 
TTGCACTGCT TGATCAGATA TATGCTTGAG CCGGACCAGG AGAAAAGACC AGACATCTAC 
CAAGTGTCCT ACTTTGCCTT TAAATTAGCT GGAAGAGACT GCCCAGTCCC AAACCTCTCA 
AACTCTCCCA TCCCAACATC TCTTCCTGAG CCTCTAACAG CCAGTGAGGT GGCTGCTAAG 
AAGAGTATGA CTAAAGCCAG GATAACCGAC TCCGTGGGTC CAACAGAAAC ATCCATAGCT 
CCCAGGCAGA GGCCGAAGGC TGCAAACAGC AACGTCCTGC CTCTCCCCAA CACAGTCACT 
CCTGTCAAAA TGACCGTGCC TTCAGCACCC GTCAGCAACG GCCAGAAAGC TCCCACTCCT 
GGATCGGGGC AGCCGACTGT GCAGACGGCG CCCAGCAGCC AGCAGCACCG CGTCCTGCAG 
CAGCTGCAGC CTGGGGACCT GCGTCTGCAG CAGCTTCAAC AACAACAAGC AGTGCAGCAG 
CAACATGCTA ATGCTCAGCA TCTGCAATAC TTGCAGTACC AACAGGCAGT GCAGCAGTCC 
CTGCAGCTTC AGCACCAGCA GGCTCTGCTC CAGCAGCAGC AGCAGCAGAT GATGATGCAG 
CCCATGTATC AGCAGCAAGT CGCTCAGGCC CAATATGCTG CCTTGCTGCA CCAGTACCAA 
CAGGCTTTTG TCCAGCAGCA GCAGCACCAC CATCATCATC ATCAGCAGCA GCAGCTTCCC 
GCTCAGCAGC CTCTTCCCTA TATGTCTTCC CCTCTTGAGT TCCAGATCCC TCTGGCTTCT 
CTAAACGCCA CCACGCCCAC TGCTGGATCC GGAGCTGCAC TTGGACCTGG ATCTGGACCA 
TCCCCTGTTG AAACCTCATA CACTAACCCC AGTAGAAACG CCCTGCTGGC CACAGATGGT 
ATCACCCCAA CCTCTCAGAG CAGCAGCGCC GTCAGTCACC CCCCCGACAT GTCGGGCTGG 
AACCCTTTTG GAGAGGACAA CTTCTCCAAA CTAACGGAGG AGGAGCTGAT CGACAGAGAG 
TTCGACCTGC TCAGAGCAAA GAAGCCAGAG GAGAGGACAG CCGGCGTGGA AACGGAGCCA 
TCGCCATCTA CAGCTCTTCC ACCGGAGGAT CTGTTCGGCT CAGTCCCATT CGTGGCCAAC 
GCAGGACCTG CAGTGATGAG CAGTGAGCAG CCTGTGGAGG TCGTCTCCAA CATCCCAGCT 
CCCATCAAGG ACCCGCACTC AGTCAAGGAG TCCAAACCAG GAAAGAAGAA CAGCTGTAAA 
CCTGCAGATG AGTCCGACAG CGACTTCGAG TCTGACCCGC CTTCGCCTAA GAGCAGTGAG 
GATGAAGAGC AGGAGGAAGA GGAGGCCTTG AACAGTGAAC ACGGTGAAGA CACAGAGCCA 
GAGAACCTTG GACAGAGGCC TCTGCTGATG GACTCTGAGG AAGAGGAGGA CGAGGACAAA 
CACAGCTCAG ATTCAGACTG TGATGCCAAC AAGGTTAAAA TCCGCTCAAA GAAACCTGCA 
GCCGCTAAAG TAGCCGATCC CAGTGCTTCC AGGAGTCCAC TAGGGGGGTC TAGGACCAGT 
CTGGTCACCC CTCCAGTGTC GCCCAGTGGA GCCAGAAACA CTGACGTTTT TGGTGCCATC 
CCTTTTGTCA GAGGAGCTTC ACCAGCGAGC CCCTCAGAGA TCACTGATAT CTTCACTAAA 
GCACCTTTCA GGCAGGTCAG TCAGGAAGAC CCGGCCGCCG ACGATTTTGA TGTTTTTACC 
AAAGCCCCGT TCAGTCGGAA TGTTTCCAAG TCGGGAAGGA GCGGCAGCGA TGTCACCATG 
GGACAAACGC CGCCGGTATC TCCCGAAGGT ACCGACGTCT TCGGCTTCTC GCCTTTTCAG 
CCGGGCCCCT CAGAGCCTCC TCCCACCACC TCCAGGAGCG CAGAGGACAT CTTCGAGTTG 
CCATTTGAGG ATTCGGCAAA CCTGCAGAGG CAGAAGCAAC GCAGTCTGCA GAAGCTGTCA 
TCACGCCAGA GAAAAACCAA GCAGGAGGCG ACGGCGGGGG GTGGCAACGG CAAGCGGCAT 
CACAGCACCC CGACCGGAGG ACGGAAGGGC AACAAGCCGA CCTACCGCAC CCCAGAGAGG 
GTCCGCAGGC ACAAGAAGGT GGGCCGCAGA GATTCTCAGA GCAGCAACGA GTTCCTGAGT 
ACCTCCGACT CCAAGGAGAA CATCAGCGTG GACATCAGCA TGGCTGAAGG GAAAGACAAA 
GCAGCGCCTC TGCCGGTGGA CGATGCTCTG TTGGACCTCT TTGGGGCCAA ACCGTTCCAC 
CCTCCAGACA GCAGCCGCCT CGGCCAGTAC CCCCAGCTCA GCGACGCCAG GGGTGACATG 
AGCACCGCTA ACGGGAAGTC ATGGAGTGGC GGCGAAAGCA GGAGCATGGA TGATTTCGGG 
GCGGTGCCCT TCACAGAACT GGTGATTCAC AGTGGACCGC AGCAGCCCGC GGCGCCTTCG 
CAGCCGCTGG ACCTCGACCC TTTTGGGGCG GCGCCGTTTC CCTCCAAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015229100.1
CDS	636..3989
Translation	MKKFSRMQKSESGGLGGPSGSSSSSGVSSYFGKVFAVGRYQVTVEELVAEGGFSVVFLARTHSGVRCALKRMYVNNVPDLNVYKREITIMKELSGHKNIVRYLDSTISSVSDSVWEVLILMEYCKAGQVVKQMNQRLNVGFSEAEVLHIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDQGNYVLCDFGSSTHKILLPLKDGVTAVEDEIKKYTTLSYRAPEMINLYAGKAITTKADIWALGCLLYKLCFFTLPFGESQVAICDGSFIVPDNSKFSTKLHCLIRYMLEPDQEKRPDIYQVSYFAFKLAGRDCPVPNLSNSPIPTSLPEPLTASEVAAKKSMTKARITDSVGPTETSIAPRQRPKAANSNVLPLPNTVTPVKMTVPSAPVSNGQKAPTPGSGQPTVQTAPSSQQHRVLQQLQPGDLRLQQLQQQQAVQQQHANAQHLQYLQYQQAVQQSLQLQHQQALLQQQQQQMMMQPMYQQQVAQAQYAALLHQYQQAFVQQQQHHHHHHQQQQLPAQQPLPYMSSPLEFQIPLASLNATTPTAGSGAALGPGSGPSPVETSYTNPSRNALLATDGITPTSQSSSAVSHPPDMSGWNPFGEDNFSKLTEEELIDREFDLLRAKKPEERTAGVETEPSPSTALPPEDLFGSVPFVANAGPAVMSSEQPVEVVSNIPAPIKDPHSVKESKPGKKNSCKPADESDSDFESDPPSPKSSEDEEQEEEEALNSEHGEDTEPENLGQRPLLMDSEEEEDEDKHSSDSDCDANKVKIRSKKPAAAKVADPSASRSPLGGSRTSLVTPPVSPSGARNTDVFGAIPFVRGASPASPSEITDIFTKAPFRQVSQEDPAADDFDVFTKAPFSRNVSKSGRSGSDVTMGQTPPVSPEGTDVFGFSPFQPGPSEPPPTTSRSAEDIFELPFEDSANLQRQKQRSLQKLSSRQRKTKQEATAGGGNGKRHHSTPTGGRKGNKPTYRTPERVRRHKKVGRRDSQSSNEFLSTSDSKENISVDISMAEGKDKAAPLPVDDALLDLFGAKPFHPPDSSRLGQYPQLSDARGDMSTANGKSWSGGESRSMDDFGAVPFTELVIHSGPQQPAAPSQPLDLDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_015373614.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015373614.1

ORF Insert Sequence:

ATGAAGAAGT TTTCCAGAAT GCAAAAGTCC GAGAGCGGAG GACTCGGAGG GCCGTCCGGT 
TCCAGCTCCA GCTCGGGGGT CAGCAGCTAC TTCGGGAAAG TGTTCGCAGT GGGCCGGTAT 
CAGGTCACCG TGGAGGAGCT CGTCGCTGAA GGGGGCTTTT CTGTGGTGTT CCTGGCTCGG 
ACGCATAGCG GCGTCCGCTG CGCTCTTAAA AGAATGTACG TGAACAACGT GCCGGACCTG 
AATGTCTACA AGAGAGAGAT CACCATCATG AAAGAGCTGT CGGGCCACAA GAACATTGTC 
CGTTACCTGG ACTCGACAAT CAGCTCGGTT TCAGACAGTG TGTGGGAGGT CCTGATTCTC 
ATGGAGTACT GCAAAGCGGG TCAGGTAGTG AAGCAGATGA ACCAGCGGTT AAATGTGGGT 
TTCAGTGAAG CCGAGGTCCT CCACATCTTC TGTGACACAT GTGAAGCTGT GGCTCGCCTT 
CATCAATGCA AGACCCCCAT CATTCACAGG GACCTTAAGG TTGAAAACAT CCTTCTGAAC 
GACCAGGGAA ACTACGTCCT GTGTGACTTC GGCAGCTCCA CTCATAAGAT CCTGTTGCCT 
CTTAAAGACG GAGTGACGGC CGTGGAGGAT GAGATAAAGA AGTACACAAC CCTCTCATAT 
CGGGCCCCGG AGATGATTAA CTTGTACGCA GGAAAAGCCA TCACCACTAA AGCTGATATC 
TGGGCACTCG GATGCTTGTT GTACAAGCTG TGTTTCTTCA CACTTCCATT TGGGGAGAGT 
CAGGTGGCCA TATGTGACGG ATCCTTTATC GTCCCGGATA ACTCAAAGTT CTCCACCAAG 
TTGCACTGCT TGATCAGATA TATGCTTGAG CCGGACCAGG AGAAAAGACC AGACATCTAC 
CAAGTGTCCT ACTTTGCCTT TAAATTAGCT GGAAGAGACT GCCCAGTCCC AAACCTCTCA 
AACTCTCCCA TCCCAACATC TCTTCCTGAG CCTCTAACAG CCAGTGAGGT GGCTGCTAAG 
AAGAGTATGA CTAAAGCCAG GATAACCGAC TCCGTGGGTC CAACAGAAAC ATCCATAGCT 
CCCAGGCAGA GGCCGAAGGC TGCAAACAGC AACGTCCTGC CTCTCCCCAA CACAGTCACT 
CCTGTCAAAA TGACCGTGCC TTCAGCACCC GTCAGCAACG GCCAGAAAGC TCCCACTCCT 
GGATCGGGGC AGCCGACTGT GCAGACGGCG CCCAGCAGCC AGCAGCACCG CGTCCTGCAG 
CAGCTGCAGC CTGGGGACCT GCGTCTGCAG CAGCTTCAAC AACAACAAGC AGTGCAGCAG 
CAACATGCTA ATGCTCAGCA TCTGCAATAC TTGCAGTACC AACAGGCAGT GCAGCAGTCC 
CTGCAGCTTC AGCACCAGCA GGCTCTGCTC CAGCAGCAGC AGCAGCAGAT GATGATGCAG 
CCCATGTATC AGCAGCAAGT CGCTCAGGCC CAATATGCTG CCTTGCTGCA CCAGTACCAA 
CAGGCTTTTG TCCAGCAGCA GCAGCACCAC CATCATCATC ATCAGCAGCA GCAGCTTCCC 
GCTCAGCAGC CTCTTCCCTA TATGTCTTCC CCTCTTGAGT TCCAGATCCC TCTGGCTTCT 
CTAAACGCCA CCACGCCCAC TGCTGGATCC GGAGCTGCAC TTGGACCTGG ATCTGGACCA 
TCCCCTGTTG AAACCTCATA CACTAACCCC AGTAGAAACG CCCTGCTGGC CACAGATGGT 
ATCACCCCAA CCTCTCAGAG CAGCAGCGCC GTCAGTCACC CCCCCGACAT GTCGGGCTGG 
AACCCTTTTG GAGAGGACAA CTTCTCCAAA CTAACGGAGG AGGAGCTGAT CGACAGAGAG 
TTCGACCTGC TCAGAGCAAA GAAGCCAGAG GAGAGGACAG CCGGCGTGGA AACGGAGCCA 
TCGCCATCTA CAGCTCTTCC ACCGGAGGAT CTGTTCGGCT CAGTCCCATT CGTGGCCAAC 
GCAGGACCTG CAGTGATGAG CAGTGAGCAG CCTGTGGAGG TCGTCTCCAA CATCCCAGCT 
CCCATCAAGG ACCCGCACTC AGTCAAGGAG TCCAAACCAG GAAAGAAGAA CAGCTGTAAA 
CCTGCAGATG AGTCCGACAG CGACTTCGAG TCTGACCCGC CTTCGCCTAA GAGCAGTGAG 
GATGAAGAGC AGGAGGAAGA GGAGGCCTTG AACAGTGAAC ACGGTGAAGA CACAGAGCCA 
GAGAACCTTG GACAGAGGCC TCTGCTGATG GACTCTGAGG AAGAGGAGGA CGAGGACAAA 
CACAGCTCAG ATTCAGACTG TGATGCCAAC AAGGTTAAAA TCCGCTCAAA GAAACCTGCA 
GCCGCTAAAG TAGCCGATCC CAGTGCTTCC AGGAGTCCAC TAGGGGGGTC TAGGACCAGT 
CTGGTCACCC CTCCAGTGTC GCCCAGTGGA GCCAGAAACA CTGACGTTTT TGGTGCCATC 
CCTTTTGTCA GAGGAGCTTC ACCAGCGAGC CCCTCAGAGA TCACTGATAT CTTCACTAAA 
GCACCTTTCA GGCAGGTCAG TCAGGAAGAC CCGGCCGCCG ACGATTTTGA TGTTTTTACC 
AAAGCCCCGT TCAGTCGGAA TGTTTCCAAG TCGGGAAGGA GCGGCAGCGA TGTCACCATG 
GGACAAACGC CGCCGGTATC TCCCGAAGGT ACCGACGTCT TCGGCTTCTC GCCTTTTCAG 
CCGGGCCCCT CAGAGCCTCC TCCCACCACC TCCAGGAGCG CAGAGGACAT CTTCGAGTTG 
CCATTTGAGG ATTCGGCAAA CCTGCAGAGG CAGAAGCAAC GCAGTCTGCA GAAGCTGTCA 
TCACGCCAGA GAAAAACCAA GCAGGAGGCG ACGGCGGGGG GTGGCAACGG CAAGCGGCAT 
CACAGCACCC CGACCGGAGG ACGGAAGGGC AACAAGCCGA CCTACCGCAC CCCAGAGAGG 
GTCCGCAGGC ACAAGAAGGT GGGCCGCAGA GATTCTCAGA GCAGCAACGA GTTCCTGAGT 
ACCTCCGACT CCAAGGAGAA CATCAGCGTG GACATCAGCA TGGCTGAAGG GAAAGACAAA 
GCAGCGCCTC TGCCGGTGGA CGATGCTCTG TTGGACCTCT TTGGGGCCAA ACCGTTCCAC 
CCTCCAGACA GCAGCCGCCT CGGCCAGTAC CCCCAGCTCA GCGACGCCAG GGGTGACATG 
AGCACCGCTA ACGGGAAGTC ATGGAGTGGC GGCGAAAGCA GGAGCATGGA TGATTTCGGG 
GCGGTGCCCT TCACAGAACT GGTGATTCAC AGTGGACCGC AGCAGCCCGC GGCGCCTTCG 
CAGCCGCTGG ACCTCGACCC TTTTGGGGCG GCGCCGTTTC CCTCCAAGCA GTAG