LOC101221376 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101221376
Entrez Gene ID	101221376
Full Name	probable prolyl 4-hydroxylase 3
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031880489.1	XP_031736349.1	probable prolyl 4-hydroxylase 10
XM_011651795.1	XP_011650097.1	probable prolyl 4-hydroxylase 3

Pathways from BioSystems
Gene Ontology

The following LOC101221376 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101221376 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu146025	XM_031880489.1 Latest version!	Cucumis sativus probable prolyl 4-hydroxylase 10 (LOC101221376), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OCu58151	XM_011651795.1 Latest version!	Cucumis sativus probable prolyl 4-hydroxylase 3 (LOC101221376), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101221376 ( XM_031880489.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_031736349.1 Cucumis sativus probable prolyl 4-hydroxylase 10 (LOC101221376), mRNA.

Price & Availability↑

CloneID	OCu146025
Clone ID Related Accession (Same CDS sequence)	XM_031880489.1
Accession Version	XM_031880489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	probable prolyl 4-hydroxylase 10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026656.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAT CACTTGTGAG TGCCCAAAAT ACTAATTGGG AAGGTGTAGT GAGTAGCCGT 
CGCACCAGTT CAGGTAGGTT TCTTGCTAAA GGGCAGAACC AACTCGTCCG TAGAATAGAG 
AAAAGAATAG CAGAATTTAC ATTCATTCCC GTAGAAAATG GAGAAGGATT GAGTATTCTA 
CATTATGAAG TTGGGCAGAA GTTTGAACCT CATCATGATT ACACTCATCC TGATTCATTC 
AGCTTTAAAA GTTTGGGCCA AAGAAATGCC ACCCTCGTCA TGTATCTGTC GGGTGTCAAA 
GAAGGGGGTG CGACGGTATT CCCGGAGGCG AAAAAATGCG CCAGCTCTGC ACGACGATGG 
TGGAAGAAAC TGCCTGAGTA TGGTAAAGAT AATGGACTCT CCGTAAAACC AAAGATGGGA 
GATGCTTTAT TGTTTTGGAG CGTGAAACCT GATGGTACAT TGGATCCTAC AAGTTTGCAT 
GCTTCTTCTC CAGTTGTAAA GGGAGACAAA TGGGTTGGTG TAAAGCTGAT GCATGTTAAA 
GCTAAAGATT TAACTCAAGA AGTTATGTAT ATATTTATAA TTCTTAATTG TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031736349.1
CDS	184..780
Translation	MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHDYTHPDSFSFKSLGQRNATLVMYLSGVKEGGATVFPEAKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGDKWVGVKLMHVKAKDLTQEVMYIFIILNCI

Target ORF information:

RefSeq Version	XM_031880489.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable prolyl 4-hydroxylase 10 (LOC101221376), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031880489.1

ORF Insert Sequence:

ATGGAGAGAT CACTTGTGAG TGCCCAAAAT ACTAATTGGG AAGGTGTAGT GAGTAGCCGT 
CGCACCAGTT CAGGTAGGTT TCTTGCTAAA GGGCAGAACC AACTCGTCCG TAGAATAGAG 
AAAAGAATAG CAGAATTTAC ATTCATTCCC GTAGAAAATG GAGAAGGATT GAGTATTCTA 
CATTATGAAG TTGGGCAGAA GTTTGAACCT CATCATGATT ACACTCATCC TGATTCATTC 
AGCTTTAAAA GTTTGGGCCA AAGAAATGCC ACCCTCGTCA TGTATCTGTC GGGTGTCAAA 
GAAGGGGGTG CGACGGTATT CCCGGAGGCG AAAAAATGCG CCAGCTCTGC ACGACGATGG 
TGGAAGAAAC TGCCTGAGTA TGGTAAAGAT AATGGACTCT CCGTAAAACC AAAGATGGGA 
GATGCTTTAT TGTTTTGGAG CGTGAAACCT GATGGTACAT TGGATCCTAC AAGTTTGCAT 
GCTTCTTCTC CAGTTGTAAA GGGAGACAAA TGGGTTGGTG TAAAGCTGAT GCATGTTAAA 
GCTAAAGATT TAACTCAAGA AGTTATGTAT ATATTTATAA TTCTTAATTG TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101221376 ( XM_011651795.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011650097.1 Cucumis sativus probable prolyl 4-hydroxylase 3 (LOC101221376), mRNA.

Price & Availability↑

CloneID	OCu58151
Clone ID Related Accession (Same CDS sequence)	XM_011651795.1
Accession Version	XM_011651795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	probable prolyl 4-hydroxylase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGTACATAC CTAGAGCTTT CATTTACCAT AATTTCTTGT CGGAGAAAGA ATGCTCGCAG 
TTGATTAATC TTGCAAAGCC TCGCATGGAG AGATCACTTG TGAGTGCCCA AAATACTAAT 
TGGGAAGGTG TAGTGAGTAG CCGTCGCACC AGTTCAGGTA GGTTTCTTGC TAAAGGGCAG 
AACCAACTCG TCCGTAGAAT AGAGAAAAGA ATAGCAGAAT TTACATTCAT TCCCGTAGAA 
AATGGAGAAG GATTGAGTAT TCTACATTAT GAAGTTGGGC AGAAGTTTGA ACCTCATCAT 
GATTACACTC ATCCTGATTC ATTCAGCTTT AAAAGTTTGG GCCAAAGAAA TGCCACCCTC 
GTCATGTATC TGTCGGGTGT CAAAGAAGGG GGTGCGACGG TATTCCCGGA GGCGAAAAAA 
TGCGCCAGCT CTGCACGACG ATGGTGGAAG AAACTGCCTG AGTATGGTAA AGATAATGGA 
CTCTCCGTAA AACCAAAGAT GGGAGATGCT TTATTGTTTT GGAGCGTGAA ACCTGATGGT 
ACATTGGATC CTACAAGTTT GCATGCTTCT TCTCCAGTTG TAAAGGGAGA CAAATGGGTT 
GGTGTAAAGC TGATGCATGT TAAAGCTAAA GATTTAACTC AAGAAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011650097.1
CDS	1..651
Translation	MYIPRAFIYHNFLSEKECSQLINLAKPRMERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHDYTHPDSFSFKSLGQRNATLVMYLSGVKEGGATVFPEAKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGDKWVGVKLMHVKAKDLTQEG

Target ORF information:

RefSeq Version	XM_011651795.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable prolyl 4-hydroxylase 3 (LOC101221376), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011651795.1

ORF Insert Sequence:

ATGTACATAC CTAGAGCTTT CATTTACCAT AATTTCTTGT CGGAGAAAGA ATGCTCGCAG 
TTGATTAATC TTGCAAAGCC TCGCATGGAG AGATCACTTG TGAGTGCCCA AAATACTAAT 
TGGGAAGGTG TAGTGAGTAG CCGTCGCACC AGTTCAGGTA GGTTTCTTGC TAAAGGGCAG 
AACCAACTCG TCCGTAGAAT AGAGAAAAGA ATAGCAGAAT TTACATTCAT TCCCGTAGAA 
AATGGAGAAG GATTGAGTAT TCTACATTAT GAAGTTGGGC AGAAGTTTGA ACCTCATCAT 
GATTACACTC ATCCTGATTC ATTCAGCTTT AAAAGTTTGG GCCAAAGAAA TGCCACCCTC 
GTCATGTATC TGTCGGGTGT CAAAGAAGGG GGTGCGACGG TATTCCCGGA GGCGAAAAAA 
TGCGCCAGCT CTGCACGACG ATGGTGGAAG AAACTGCCTG AGTATGGTAA AGATAATGGA 
CTCTCCGTAA AACCAAAGAT GGGAGATGCT TTATTGTTTT GGAGCGTGAA ACCTGATGGT 
ACATTGGATC CTACAAGTTT GCATGCTTCT TCTCCAGTTG TAAAGGGAGA CAAATGGGTT 
GGTGTAAAGC TGATGCATGT TAAAGCTAAA GATTTAACTC AAGAAGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.