HMA5.2 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMA5.2
Entrez Gene ID	101218801
Full Name	probable copper-transporting ATPase HMA5
Synonyms	HMA5B-2
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011655157.1	XP_011653459.1	probable copper-transporting ATPase HMA5
XM_011655157.2	XP_011653459.1	probable copper-transporting ATPase HMA5

Pathways from BioSystems
Gene Ontology

The following HMA5.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMA5.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu63925	XM_011655157.2 Latest version!	Cucumis sativus probable copper-transporting ATPase HMA5 (HMA5.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCu63925	XM_011655157.1	Cucumis sativus probable copper-transporting ATPase HMA5 (LOC101218801), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMA5.2 ( XM_011655157.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011653459.1 Cucumis sativus probable copper-transporting ATPase HMA5 (LOC101218801), mRNA.

Price & Availability↑

CloneID	OCu63925
Clone ID Related Accession (Same CDS sequence)	XM_011655157.1 , XM_011655157.2
Accession Version	XM_011655157.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	probable copper-transporting ATPase HMA5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953806.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA ATTTCTGGTC CTTAGCATGT ATTCGTAGTC CCAACACCAC CAATCTCTCT 
CCACGCCCTC ATTATCCCTC TATGCCCAAA TACCCAGCTG GGGTTTCCCA GCCCGAGAAT 
AGCTTACCGG TGATCGAGTC CACCGCTTTT TTCTCCGTCA CCGGAATGAC CTGCTCTGCT 
TGTGCTGGCT CTGTCGAGAA AGCCATCAAA CGTCTTCCTG GGATTCGTGA AGCCGTCGTT 
GGTGTTTTGA ATGCCAAAGC TCGTGTCCAG TTTTACCCTA GCTTCGTTAA TGTGGATCAA 
ATATGTGAGG CAATTAATGA TGCAGGCTTT GAAGCAAGTG TTGTCAATGA TGATATGATT 
GAAAGATGTC GAATTCGTGT AATTGGGATG ACTTGCACTT CGTGTTCCAC AACTTTGGAA 
TCTACATTAC TAGCAATTGG TGGAGTTCAA AACGCTCAAG TTGCATTAGC CACGGAAGAA 
GCAGAAATTT GTTATGATCC AAGGATTTTG AACTACAATC AACTTCTCCA AGCCATTGAA 
GATTCTGGAT TTGAAGCTAT ACTTATTAGT ACAGAAGAAG ATGTCAGCAA GATACAGCTC 
CATGTTGAGG GAGTAAGAAC TGAGAATTCA ATGAGATTGA TTGGGAGCTC TCTGGAAGCT 
CTTCCTGGAG TCTTAGGTAT TGATATTGAA CCTGCCGTCA ATAAACTTTC CTTGTCTTAT 
AAACCAAATA TCACGGGACC TCGGAATGTA ATCCAAGTGA TTGAATCAAC TGGATCGGGT 
CGATACAAGG CAACAATATT TCCTGAAGGA GAGGGCAGAG AAGCTTATAA AAAGGAGGAA 
ATTAAGCAAT ATTACAGATC GTTCTTATGG AGCCTGATAT TTACAATTCC AGTATTCCTA 
AGCTCCATGG TCTTCACTTA TATACCTGGT ATTAAGGAAG GGCTAGATAC TAAAGTTGTC 
AATATGATGA CTGTTGGAGA GCTGCTGAGA TGGGTGTTAT CAACTCCTGT ACAGTTCATT 
ATTGGACGGC GGTTCTATAC TGGGTCTTAT AAAGCATTGC GTCATGGTTC TGCAAATATG 
GATGTCTTGA TTGCTTTGGG GACAAATGCA GCATACTTTT ATTCAGTCTA CATGGTCTTA 
AGATCTGCAA CCTCAAGTGA TTTTAAGGCC ACGGACTTCT TTGAGACCAG TTCCATGCTT 
ATTTCATTCA TTCTCCTTGG GAAATATCTT GAGGTCTTGG CAAAGGGAAA GACATCAGAG 
GCTATTGCCA AGCTTATGAA GTTGGTGCCT GAAACAGCGA CGCTGCTTAC TTTTGATGAT 
GATGGACATA TCATTAGGGA AGAAGAAATT GATAGTCGGT TGATACAAAA GAATGATGTG 
ATTAAAGTCA TTCCAGGGGC AAAAGTTGCT TCAGATGGAA TTGTTGTTTG GGGACAAAGT 
CATGTCAATG AAAGCATGAT TACTGGGGAA GCCAAGCCAG TGGCAAAAAG AAAGGATGAT 
ACAGTGATTG GAGGCACCTT GAACGAGAAC GGGGTGCTAC ATGTGAGAGC AACACATGTT 
GGATCTGAAA GTGCTCTTTC ACAGATTGTG CGACTTGTTG AATCCGCTCA GATGGCCAAG 
GCTCCTGTAC AGAAAATTGC AGATCGCATC TCTAAGGTTT TTGTGCCAAT GGTAATAGTT 
CTTTCATTGA CAACGTGGCT CGTCTGGTTC CTCACTGGGA AGTATGGTGG CTACCCAAGG 
ACTTGGATAC CTTCTTCTAT GGATAGCTTT GAACTTGCTC TCCAGTTTGG TATCTCAGTT 
ATGGTCATAG CATGCCCTTG TGCTTTGGGC CTGGCAACTC CAACCGCTGT CATGGTTGGA 
ACAGGAGTTG GGGCATCAAA AGGTGTTTTA ATCAAAGGCG GCCAAGCATT AGAGAGTGCT 
CACAAGGTGA ATTGCATTGT GTTTGACAAG ACTGGAACTC TTACAGTCGG GAAGCCAGTT 
GTGGTGAACA CCAAGCTACT TAAAAATATG GCTTTGAAAG AATTCTGTGT ACTAGTCGCC 
GCCACTGAGG TTAACAGTGA GCATCCACTG GCTAAAGCCG TTGTCGAATA TGCTCAAAAG 
TTCAAAGAAG AAGATGATAA CAAAACTTGG CCTGAAGCAC AAGACTTTAT CTCCATTACT 
GGACATGGAG TTAAGGCCAT CGTTCAAAAC AAAGAAGTAC TTGTAGGCAA CAAGAGTCTT 
ATGTTAGATC AGAACATCTT GATACCTATT GAAGCTGAAG AAATATTAAA AGAAATTGAA 
GAAATGGCGC AAACTGGAAT TTTGATATCC ATTGATAGGA AATTGACAGG AGTTCTTGCC 
ATATCTGATC CACTAAAACC AAGTGCTCGA GAGGTCATTT CGATACTCAA GGCCATGAAA 
GTTAAATCTA TCATGGTGAC AGGTGATAAT TGGGGAACTG CAAAGTCCAT AGCCAAAGAG 
GTTGGGATTG ATGATGTAAC TGCAGAAGCT AAACCTGACC AGAAAGCTGA TGAAGTGAAG 
AGATTACAGT CTTTGGGTCA TACGGTGGCA ATGGTTGGAG ACGGTATCAA CGACTCACCA 
GCACTTGTGG CTGCAGATGT TGGAATGGCA ATTGGTGCAG GCACAGACAT CGCCATTGAG 
GCAGCTGACA TCGTTCTCAT GAAAAGCAAC TTAGAGGATG TCATAACCGC CATCGACCTT 
TCGAGGAAAA CCTTTTCTAG GATCCGTCTA AACTACATTT GGGCGCTCGG TTACAATCTC 
CTCGGCATCC CTATCGCAGC AGGAGTCCTA TTCCCATCAA CTCGGTTTCG GTTACCACCA 
TGGATTGCAG GAGCAGCAAT GGCAGCCTCT TCTGTGAGTG TTGTTTGTAG TTCTCTCCTT 
CTCAAGTATT ACAAAAGACC TAAGAAGCTT GATACACTTG AGATACAAGG CATTAGAGTT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011653459.1
CDS	124..3069
Translation	MASNFWSLACIRSPNTTNLSPRPHYPSMPKYPAGVSQPENSLPVIESTAFFSVTGMTCSACAGSVEKAIKRLPGIREAVVGVLNAKARVQFYPSFVNVDQICEAINDAGFEASVVNDDMIERCRIRVIGMTCTSCSTTLESTLLAIGGVQNAQVALATEEAEICYDPRILNYNQLLQAIEDSGFEAILISTEEDVSKIQLHVEGVRTENSMRLIGSSLEALPGVLGIDIEPAVNKLSLSYKPNITGPRNVIQVIESTGSGRYKATIFPEGEGREAYKKEEIKQYYRSFLWSLIFTIPVFLSSMVFTYIPGIKEGLDTKVVNMMTVGELLRWVLSTPVQFIIGRRFYTGSYKALRHGSANMDVLIALGTNAAYFYSVYMVLRSATSSDFKATDFFETSSMLISFILLGKYLEVLAKGKTSEAIAKLMKLVPETATLLTFDDDGHIIREEEIDSRLIQKNDVIKVIPGAKVASDGIVVWGQSHVNESMITGEAKPVAKRKDDTVIGGTLNENGVLHVRATHVGSESALSQIVRLVESAQMAKAPVQKIADRISKVFVPMVIVLSLTTWLVWFLTGKYGGYPRTWIPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASKGVLIKGGQALESAHKVNCIVFDKTGTLTVGKPVVVNTKLLKNMALKEFCVLVAATEVNSEHPLAKAVVEYAQKFKEEDDNKTWPEAQDFISITGHGVKAIVQNKEVLVGNKSLMLDQNILIPIEAEEILKEIEEMAQTGILISIDRKLTGVLAISDPLKPSAREVISILKAMKVKSIMVTGDNWGTAKSIAKEVGIDDVTAEAKPDQKADEVKRLQSLGHTVAMVGDGINDSPALVAADVGMAIGAGTDIAIEAADIVLMKSNLEDVITAIDLSRKTFSRIRLNYIWALGYNLLGIPIAAGVLFPSTRFRLPPWIAGAAMAASSVSVVCSSLLLKYYKRPKKLDTLEIQGIRVE

Target ORF information:

RefSeq Version	XM_011655157.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable copper-transporting ATPase HMA5 (LOC101218801), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011655157.1

ORF Insert Sequence:

ATGGCTTCCA ATTTCTGGTC CTTAGCATGT ATTCGTAGTC CCAACACCAC CAATCTCTCT 
CCACGCCCTC ATTATCCCTC TATGCCCAAA TACCCAGCTG GGGTTTCCCA GCCCGAGAAT 
AGCTTACCGG TGATCGAGTC CACCGCTTTT TTCTCCGTCA CCGGAATGAC CTGCTCTGCT 
TGTGCTGGCT CTGTCGAGAA AGCCATCAAA CGTCTTCCTG GGATTCGTGA AGCCGTCGTT 
GGTGTTTTGA ATGCCAAAGC TCGTGTCCAG TTTTACCCTA GCTTCGTTAA TGTGGATCAA 
ATATGTGAGG CAATTAATGA TGCAGGCTTT GAAGCAAGTG TTGTCAATGA TGATATGATT 
GAAAGATGTC GAATTCGTGT AATTGGGATG ACTTGCACTT CGTGTTCCAC AACTTTGGAA 
TCTACATTAC TAGCAATTGG TGGAGTTCAA AACGCTCAAG TTGCATTAGC CACGGAAGAA 
GCAGAAATTT GTTATGATCC AAGGATTTTG AACTACAATC AACTTCTCCA AGCCATTGAA 
GATTCTGGAT TTGAAGCTAT ACTTATTAGT ACAGAAGAAG ATGTCAGCAA GATACAGCTC 
CATGTTGAGG GAGTAAGAAC TGAGAATTCA ATGAGATTGA TTGGGAGCTC TCTGGAAGCT 
CTTCCTGGAG TCTTAGGTAT TGATATTGAA CCTGCCGTCA ATAAACTTTC CTTGTCTTAT 
AAACCAAATA TCACGGGACC TCGGAATGTA ATCCAAGTGA TTGAATCAAC TGGATCGGGT 
CGATACAAGG CAACAATATT TCCTGAAGGA GAGGGCAGAG AAGCTTATAA AAAGGAGGAA 
ATTAAGCAAT ATTACAGATC GTTCTTATGG AGCCTGATAT TTACAATTCC AGTATTCCTA 
AGCTCCATGG TCTTCACTTA TATACCTGGT ATTAAGGAAG GGCTAGATAC TAAAGTTGTC 
AATATGATGA CTGTTGGAGA GCTGCTGAGA TGGGTGTTAT CAACTCCTGT ACAGTTCATT 
ATTGGACGGC GGTTCTATAC TGGGTCTTAT AAAGCATTGC GTCATGGTTC TGCAAATATG 
GATGTCTTGA TTGCTTTGGG GACAAATGCA GCATACTTTT ATTCAGTCTA CATGGTCTTA 
AGATCTGCAA CCTCAAGTGA TTTTAAGGCC ACGGACTTCT TTGAGACCAG TTCCATGCTT 
ATTTCATTCA TTCTCCTTGG GAAATATCTT GAGGTCTTGG CAAAGGGAAA GACATCAGAG 
GCTATTGCCA AGCTTATGAA GTTGGTGCCT GAAACAGCGA CGCTGCTTAC TTTTGATGAT 
GATGGACATA TCATTAGGGA AGAAGAAATT GATAGTCGGT TGATACAAAA GAATGATGTG 
ATTAAAGTCA TTCCAGGGGC AAAAGTTGCT TCAGATGGAA TTGTTGTTTG GGGACAAAGT 
CATGTCAATG AAAGCATGAT TACTGGGGAA GCCAAGCCAG TGGCAAAAAG AAAGGATGAT 
ACAGTGATTG GAGGCACCTT GAACGAGAAC GGGGTGCTAC ATGTGAGAGC AACACATGTT 
GGATCTGAAA GTGCTCTTTC ACAGATTGTG CGACTTGTTG AATCCGCTCA GATGGCCAAG 
GCTCCTGTAC AGAAAATTGC AGATCGCATC TCTAAGGTTT TTGTGCCAAT GGTAATAGTT 
CTTTCATTGA CAACGTGGCT CGTCTGGTTC CTCACTGGGA AGTATGGTGG CTACCCAAGG 
ACTTGGATAC CTTCTTCTAT GGATAGCTTT GAACTTGCTC TCCAGTTTGG TATCTCAGTT 
ATGGTCATAG CATGCCCTTG TGCTTTGGGC CTGGCAACTC CAACCGCTGT CATGGTTGGA 
ACAGGAGTTG GGGCATCAAA AGGTGTTTTA ATCAAAGGCG GCCAAGCATT AGAGAGTGCT 
CACAAGGTGA ATTGCATTGT GTTTGACAAG ACTGGAACTC TTACAGTCGG GAAGCCAGTT 
GTGGTGAACA CCAAGCTACT TAAAAATATG GCTTTGAAAG AATTCTGTGT ACTAGTCGCC 
GCCACTGAGG TTAACAGTGA GCATCCACTG GCTAAAGCCG TTGTCGAATA TGCTCAAAAG 
TTCAAAGAAG AAGATGATAA CAAAACTTGG CCTGAAGCAC AAGACTTTAT CTCCATTACT 
GGACATGGAG TTAAGGCCAT CGTTCAAAAC AAAGAAGTAC TTGTAGGCAA CAAGAGTCTT 
ATGTTAGATC AGAACATCTT GATACCTATT GAAGCTGAAG AAATATTAAA AGAAATTGAA 
GAAATGGCGC AAACTGGAAT TTTGATATCC ATTGATAGGA AATTGACAGG AGTTCTTGCC 
ATATCTGATC CACTAAAACC AAGTGCTCGA GAGGTCATTT CGATACTCAA GGCCATGAAA 
GTTAAATCTA TCATGGTGAC AGGTGATAAT TGGGGAACTG CAAAGTCCAT AGCCAAAGAG 
GTTGGGATTG ATGATGTAAC TGCAGAAGCT AAACCTGACC AGAAAGCTGA TGAAGTGAAG 
AGATTACAGT CTTTGGGTCA TACGGTGGCA ATGGTTGGAG ACGGTATCAA CGACTCACCA 
GCACTTGTGG CTGCAGATGT TGGAATGGCA ATTGGTGCAG GCACAGACAT CGCCATTGAG 
GCAGCTGACA TCGTTCTCAT GAAAAGCAAC TTAGAGGATG TCATAACCGC CATCGACCTT 
TCGAGGAAAA CCTTTTCTAG GATCCGTCTA AACTACATTT GGGCGCTCGG TTACAATCTC 
CTCGGCATCC CTATCGCAGC AGGAGTCCTA TTCCCATCAA CTCGGTTTCG GTTACCACCA 
TGGATTGCAG GAGCAGCAAT GGCAGCCTCT TCTGTGAGTG TTGTTTGTAG TTCTCTCCTT 
CTCAAGTATT ACAAAAGACC TAAGAAGCTT GATACACTTG AGATACAAGG CATTAGAGTT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMA5.2 ( XM_011655157.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011653459.1 Cucumis sativus probable copper-transporting ATPase HMA5 (HMA5.2), mRNA.

Price & Availability↑

CloneID	OCu63925
Clone ID Related Accession (Same CDS sequence)	XM_011655157.1 , XM_011655157.2
Accession Version	XM_011655157.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	probable copper-transporting ATPase HMA5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026658.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011655157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA ATTTCTGGTC CTTAGCATGT ATTCGTAGTC CCAACACCAC CAATCTCTCT 
CCACGCCCTC ATTATCCCTC TATGCCCAAA TACCCAGCTG GGGTTTCCCA GCCCGAGAAT 
AGCTTACCGG TGATCGAGTC CACCGCTTTT TTCTCCGTCA CCGGAATGAC CTGCTCTGCT 
TGTGCTGGCT CTGTCGAGAA AGCCATCAAA CGTCTTCCTG GGATTCGTGA AGCCGTCGTT 
GGTGTTTTGA ATGCCAAAGC TCGTGTCCAG TTTTACCCTA GCTTCGTTAA TGTGGATCAA 
ATATGTGAGG CAATTAATGA TGCAGGCTTT GAAGCAAGTG TTGTCAATGA TGATATGATT 
GAAAGATGTC GAATTCGTGT AATTGGGATG ACTTGCACTT CGTGTTCCAC AACTTTGGAA 
TCTACATTAC TAGCAATTGG TGGAGTTCAA AACGCTCAAG TTGCATTAGC CACGGAAGAA 
GCAGAAATTT GTTATGATCC AAGGATTTTG AACTACAATC AACTTCTCCA AGCCATTGAA 
GATTCTGGAT TTGAAGCTAT ACTTATTAGT ACAGAAGAAG ATGTCAGCAA GATACAGCTC 
CATGTTGAGG GAGTAAGAAC TGAGAATTCA ATGAGATTGA TTGGGAGCTC TCTGGAAGCT 
CTTCCTGGAG TCTTAGGTAT TGATATTGAA CCTGCCGTCA ATAAACTTTC CTTGTCTTAT 
AAACCAAATA TCACGGGACC TCGGAATGTA ATCCAAGTGA TTGAATCAAC TGGATCGGGT 
CGATACAAGG CAACAATATT TCCTGAAGGA GAGGGCAGAG AAGCTTATAA AAAGGAGGAA 
ATTAAGCAAT ATTACAGATC GTTCTTATGG AGCCTGATAT TTACAATTCC AGTATTCCTA 
AGCTCCATGG TCTTCACTTA TATACCTGGT ATTAAGGAAG GGCTAGATAC TAAAGTTGTC 
AATATGATGA CTGTTGGAGA GCTGCTGAGA TGGGTGTTAT CAACTCCTGT ACAGTTCATT 
ATTGGACGGC GGTTCTATAC TGGGTCTTAT AAAGCATTGC GTCATGGTTC TGCAAATATG 
GATGTCTTGA TTGCTTTGGG GACAAATGCA GCATACTTTT ATTCAGTCTA CATGGTCTTA 
AGATCTGCAA CCTCAAGTGA TTTTAAGGCC ACGGACTTCT TTGAGACCAG TTCCATGCTT 
ATTTCATTCA TTCTCCTTGG GAAATATCTT GAGGTCTTGG CAAAGGGAAA GACATCAGAG 
GCTATTGCCA AGCTTATGAA GTTGGTGCCT GAAACAGCGA CGCTGCTTAC TTTTGATGAT 
GATGGACATA TCATTAGGGA AGAAGAAATT GATAGTCGGT TGATACAAAA GAATGATGTG 
ATTAAAGTCA TTCCAGGGGC AAAAGTTGCT TCAGATGGAA TTGTTGTTTG GGGACAAAGT 
CATGTCAATG AAAGCATGAT TACTGGGGAA GCCAAGCCAG TGGCAAAAAG AAAGGATGAT 
ACAGTGATTG GAGGCACCTT GAACGAGAAC GGGGTGCTAC ATGTGAGAGC AACACATGTT 
GGATCTGAAA GTGCTCTTTC ACAGATTGTG CGACTTGTTG AATCCGCTCA GATGGCCAAG 
GCTCCTGTAC AGAAAATTGC AGATCGCATC TCTAAGGTTT TTGTGCCAAT GGTAATAGTT 
CTTTCATTGA CAACGTGGCT CGTCTGGTTC CTCACTGGGA AGTATGGTGG CTACCCAAGG 
ACTTGGATAC CTTCTTCTAT GGATAGCTTT GAACTTGCTC TCCAGTTTGG TATCTCAGTT 
ATGGTCATAG CATGCCCTTG TGCTTTGGGC CTGGCAACTC CAACCGCTGT CATGGTTGGA 
ACAGGAGTTG GGGCATCAAA AGGTGTTTTA ATCAAAGGCG GCCAAGCATT AGAGAGTGCT 
CACAAGGTGA ATTGCATTGT GTTTGACAAG ACTGGAACTC TTACAGTCGG GAAGCCAGTT 
GTGGTGAACA CCAAGCTACT TAAAAATATG GCTTTGAAAG AATTCTGTGT ACTAGTCGCC 
GCCACTGAGG TTAACAGTGA GCATCCACTG GCTAAAGCCG TTGTCGAATA TGCTCAAAAG 
TTCAAAGAAG AAGATGATAA CAAAACTTGG CCTGAAGCAC AAGACTTTAT CTCCATTACT 
GGACATGGAG TTAAGGCCAT CGTTCAAAAC AAAGAAGTAC TTGTAGGCAA CAAGAGTCTT 
ATGTTAGATC AGAACATCTT GATACCTATT GAAGCTGAAG AAATATTAAA AGAAATTGAA 
GAAATGGCGC AAACTGGAAT TTTGATATCC ATTGATAGGA AATTGACAGG AGTTCTTGCC 
ATATCTGATC CACTAAAACC AAGTGCTCGA GAGGTCATTT CGATACTCAA GGCCATGAAA 
GTTAAATCTA TCATGGTGAC AGGTGATAAT TGGGGAACTG CAAAGTCCAT AGCCAAAGAG 
GTTGGGATTG ATGATGTAAC TGCAGAAGCT AAACCTGACC AGAAAGCTGA TGAAGTGAAG 
AGATTACAGT CTTTGGGTCA TACGGTGGCA ATGGTTGGAG ACGGTATCAA CGACTCACCA 
GCACTTGTGG CTGCAGATGT TGGAATGGCA ATTGGTGCAG GCACAGACAT CGCCATTGAG 
GCAGCTGACA TCGTTCTCAT GAAAAGCAAC TTAGAGGATG TCATAACCGC CATCGACCTT 
TCGAGGAAAA CCTTTTCTAG GATCCGTCTA AACTACATTT GGGCGCTCGG TTACAATCTC 
CTCGGCATCC CTATCGCAGC AGGAGTCCTA TTCCCATCAA CTCGGTTTCG GTTACCACCA 
TGGATTGCAG GAGCAGCAAT GGCAGCCTCT TCTGTGAGTG TTGTTTGTAG TTCTCTCCTT 
CTCAAGTATT ACAAAAGACC TAAGAAGCTT GATACACTTG AGATACAAGG CATTAGAGTT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011653459.1
CDS	149..3094
Translation	MASNFWSLACIRSPNTTNLSPRPHYPSMPKYPAGVSQPENSLPVIESTAFFSVTGMTCSACAGSVEKAIKRLPGIREAVVGVLNAKARVQFYPSFVNVDQICEAINDAGFEASVVNDDMIERCRIRVIGMTCTSCSTTLESTLLAIGGVQNAQVALATEEAEICYDPRILNYNQLLQAIEDSGFEAILISTEEDVSKIQLHVEGVRTENSMRLIGSSLEALPGVLGIDIEPAVNKLSLSYKPNITGPRNVIQVIESTGSGRYKATIFPEGEGREAYKKEEIKQYYRSFLWSLIFTIPVFLSSMVFTYIPGIKEGLDTKVVNMMTVGELLRWVLSTPVQFIIGRRFYTGSYKALRHGSANMDVLIALGTNAAYFYSVYMVLRSATSSDFKATDFFETSSMLISFILLGKYLEVLAKGKTSEAIAKLMKLVPETATLLTFDDDGHIIREEEIDSRLIQKNDVIKVIPGAKVASDGIVVWGQSHVNESMITGEAKPVAKRKDDTVIGGTLNENGVLHVRATHVGSESALSQIVRLVESAQMAKAPVQKIADRISKVFVPMVIVLSLTTWLVWFLTGKYGGYPRTWIPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASKGVLIKGGQALESAHKVNCIVFDKTGTLTVGKPVVVNTKLLKNMALKEFCVLVAATEVNSEHPLAKAVVEYAQKFKEEDDNKTWPEAQDFISITGHGVKAIVQNKEVLVGNKSLMLDQNILIPIEAEEILKEIEEMAQTGILISIDRKLTGVLAISDPLKPSAREVISILKAMKVKSIMVTGDNWGTAKSIAKEVGIDDVTAEAKPDQKADEVKRLQSLGHTVAMVGDGINDSPALVAADVGMAIGAGTDIAIEAADIVLMKSNLEDVITAIDLSRKTFSRIRLNYIWALGYNLLGIPIAAGVLFPSTRFRLPPWIAGAAMAASSVSVVCSSLLLKYYKRPKKLDTLEIQGIRVE

Target ORF information:

RefSeq Version	XM_011655157.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable copper-transporting ATPase HMA5 (HMA5.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011655157.2

ORF Insert Sequence:

ATGGCTTCCA ATTTCTGGTC CTTAGCATGT ATTCGTAGTC CCAACACCAC CAATCTCTCT 
CCACGCCCTC ATTATCCCTC TATGCCCAAA TACCCAGCTG GGGTTTCCCA GCCCGAGAAT 
AGCTTACCGG TGATCGAGTC CACCGCTTTT TTCTCCGTCA CCGGAATGAC CTGCTCTGCT 
TGTGCTGGCT CTGTCGAGAA AGCCATCAAA CGTCTTCCTG GGATTCGTGA AGCCGTCGTT 
GGTGTTTTGA ATGCCAAAGC TCGTGTCCAG TTTTACCCTA GCTTCGTTAA TGTGGATCAA 
ATATGTGAGG CAATTAATGA TGCAGGCTTT GAAGCAAGTG TTGTCAATGA TGATATGATT 
GAAAGATGTC GAATTCGTGT AATTGGGATG ACTTGCACTT CGTGTTCCAC AACTTTGGAA 
TCTACATTAC TAGCAATTGG TGGAGTTCAA AACGCTCAAG TTGCATTAGC CACGGAAGAA 
GCAGAAATTT GTTATGATCC AAGGATTTTG AACTACAATC AACTTCTCCA AGCCATTGAA 
GATTCTGGAT TTGAAGCTAT ACTTATTAGT ACAGAAGAAG ATGTCAGCAA GATACAGCTC 
CATGTTGAGG GAGTAAGAAC TGAGAATTCA ATGAGATTGA TTGGGAGCTC TCTGGAAGCT 
CTTCCTGGAG TCTTAGGTAT TGATATTGAA CCTGCCGTCA ATAAACTTTC CTTGTCTTAT 
AAACCAAATA TCACGGGACC TCGGAATGTA ATCCAAGTGA TTGAATCAAC TGGATCGGGT 
CGATACAAGG CAACAATATT TCCTGAAGGA GAGGGCAGAG AAGCTTATAA AAAGGAGGAA 
ATTAAGCAAT ATTACAGATC GTTCTTATGG AGCCTGATAT TTACAATTCC AGTATTCCTA 
AGCTCCATGG TCTTCACTTA TATACCTGGT ATTAAGGAAG GGCTAGATAC TAAAGTTGTC 
AATATGATGA CTGTTGGAGA GCTGCTGAGA TGGGTGTTAT CAACTCCTGT ACAGTTCATT 
ATTGGACGGC GGTTCTATAC TGGGTCTTAT AAAGCATTGC GTCATGGTTC TGCAAATATG 
GATGTCTTGA TTGCTTTGGG GACAAATGCA GCATACTTTT ATTCAGTCTA CATGGTCTTA 
AGATCTGCAA CCTCAAGTGA TTTTAAGGCC ACGGACTTCT TTGAGACCAG TTCCATGCTT 
ATTTCATTCA TTCTCCTTGG GAAATATCTT GAGGTCTTGG CAAAGGGAAA GACATCAGAG 
GCTATTGCCA AGCTTATGAA GTTGGTGCCT GAAACAGCGA CGCTGCTTAC TTTTGATGAT 
GATGGACATA TCATTAGGGA AGAAGAAATT GATAGTCGGT TGATACAAAA GAATGATGTG 
ATTAAAGTCA TTCCAGGGGC AAAAGTTGCT TCAGATGGAA TTGTTGTTTG GGGACAAAGT 
CATGTCAATG AAAGCATGAT TACTGGGGAA GCCAAGCCAG TGGCAAAAAG AAAGGATGAT 
ACAGTGATTG GAGGCACCTT GAACGAGAAC GGGGTGCTAC ATGTGAGAGC AACACATGTT 
GGATCTGAAA GTGCTCTTTC ACAGATTGTG CGACTTGTTG AATCCGCTCA GATGGCCAAG 
GCTCCTGTAC AGAAAATTGC AGATCGCATC TCTAAGGTTT TTGTGCCAAT GGTAATAGTT 
CTTTCATTGA CAACGTGGCT CGTCTGGTTC CTCACTGGGA AGTATGGTGG CTACCCAAGG 
ACTTGGATAC CTTCTTCTAT GGATAGCTTT GAACTTGCTC TCCAGTTTGG TATCTCAGTT 
ATGGTCATAG CATGCCCTTG TGCTTTGGGC CTGGCAACTC CAACCGCTGT CATGGTTGGA 
ACAGGAGTTG GGGCATCAAA AGGTGTTTTA ATCAAAGGCG GCCAAGCATT AGAGAGTGCT 
CACAAGGTGA ATTGCATTGT GTTTGACAAG ACTGGAACTC TTACAGTCGG GAAGCCAGTT 
GTGGTGAACA CCAAGCTACT TAAAAATATG GCTTTGAAAG AATTCTGTGT ACTAGTCGCC 
GCCACTGAGG TTAACAGTGA GCATCCACTG GCTAAAGCCG TTGTCGAATA TGCTCAAAAG 
TTCAAAGAAG AAGATGATAA CAAAACTTGG CCTGAAGCAC AAGACTTTAT CTCCATTACT 
GGACATGGAG TTAAGGCCAT CGTTCAAAAC AAAGAAGTAC TTGTAGGCAA CAAGAGTCTT 
ATGTTAGATC AGAACATCTT GATACCTATT GAAGCTGAAG AAATATTAAA AGAAATTGAA 
GAAATGGCGC AAACTGGAAT TTTGATATCC ATTGATAGGA AATTGACAGG AGTTCTTGCC 
ATATCTGATC CACTAAAACC AAGTGCTCGA GAGGTCATTT CGATACTCAA GGCCATGAAA 
GTTAAATCTA TCATGGTGAC AGGTGATAAT TGGGGAACTG CAAAGTCCAT AGCCAAAGAG 
GTTGGGATTG ATGATGTAAC TGCAGAAGCT AAACCTGACC AGAAAGCTGA TGAAGTGAAG 
AGATTACAGT CTTTGGGTCA TACGGTGGCA ATGGTTGGAG ACGGTATCAA CGACTCACCA 
GCACTTGTGG CTGCAGATGT TGGAATGGCA ATTGGTGCAG GCACAGACAT CGCCATTGAG 
GCAGCTGACA TCGTTCTCAT GAAAAGCAAC TTAGAGGATG TCATAACCGC CATCGACCTT 
TCGAGGAAAA CCTTTTCTAG GATCCGTCTA AACTACATTT GGGCGCTCGG TTACAATCTC 
CTCGGCATCC CTATCGCAGC AGGAGTCCTA TTCCCATCAA CTCGGTTTCG GTTACCACCA 
TGGATTGCAG GAGCAGCAAT GGCAGCCTCT TCTGTGAGTG TTGTTTGTAG TTCTCTCCTT 
CTCAAGTATT ACAAAAGACC TAAGAAGCTT GATACACTTG AGATACAAGG CATTAGAGTT 
GAGTGA