LOC101218315 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101218315
Entrez Gene ID	101218315
Full Name	protein timeless homolog
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011656331.1	XP_011654633.1	protein timeless homolog
XM_011656331.2	XP_011654633.1	protein timeless homolog

Pathways from BioSystems
Gene Ontology

The following LOC101218315 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101218315 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu65770	XM_011656331.2 Latest version!	Cucumis sativus protein timeless homolog (LOC101218315), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCu65770	XM_011656331.1	Cucumis sativus protein timeless homolog (LOC101218315), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101218315 ( XM_011656331.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011654633.1 Cucumis sativus protein timeless homolog (LOC101218315), mRNA.

Price & Availability↑

CloneID	OCu65770
Clone ID Related Accession (Same CDS sequence)	XM_011656331.1 , XM_011656331.2
Accession Version	XM_011656331.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	protein timeless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953807.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATAG ATGGGCTATG TGTTATTTGC GCTGGCATTG GAATCGTCGA GGAGGATGAC 
TACGGCAATC GGATCGGTTA CTCCAAAAAC GAGTTCTGTT TAGATAATTT GAAGGATTTG 
CTTAGGTTCC TGAGGAGAGA TGACCCGCAG ACTCGCGATG TTTTTAAGCA CGTTTGTAAA 
TGGAACATTG TGGGTAAAGA TTTGATTCCC ATCATTGAGT ACTGTCAAGA TGATCGTAAT 
GCAGTTTTAA ATGCAGTGAA GATTTTGGTG TTCCTCACAA TGCCTATCGA ACCCACGTCA 
AGTGACATTG CACAACAGAT CGAGTATCTA TGGGGATTGA AGTCTTTAAT CACATGCAGT 
AATGTTGTTG CCATAATTGT TTCACTTCTG GAGAGTCCGC TGGAAAATCT GGATTGCGGA 
ACATTCTCAG AAGATGACTG GAAATTGTTG CAGTTGGTTA TAACACTCTT CCGAAATGTT 
TTGGCTATTC AAGAAATCTC ATTGCAGCAA AAGGCTGATG GATCAGCTTG TCAGTTAATA 
TTGCTGAGAG ACAAATTTTT GGAAGTTTTA TTCCGGGAGA ATGTTATGGA CATAATATTG 
GTGATGACGC AGCACATTGA TGGGTCTTGC AGCCATCTCC GTCAAGACAA ATTAGTCTTT 
TTGGAAATAT TTTACTACAT ATTTATGGGC CAAGAGCCTG AGTTAATCGC GAAAGTGCCT 
CAAAACAGCA GTGAGGAAAA TGTAGAAACG GTATCTTCTG TCAATAGTCT GAAATCTATG 
ATGGAGGAAG ATAGAAGAAA GTTTTCTAGA TTACAAAATT TGAACCGACA TTCGCAGTTT 
AGTGGAACTT TTACTCGGCA AACCTTGGAT GGTTCAAAGT TAGTACTCAA AGGAAAACCA 
TCTTTGAGTT CTTCTACCAG CCATAAGCCA CCTAAAGTTT GTCGAGGTCC AATTAAAAAA 
ATTGCATGGG ACCTTGGACG ATTAACATCG AAAAACAGCA AACTTCTGCA GTTACTTCAT 
GATTTTATTA ATCAGTTTCT TTCAGGCGGC TACAATGCGC TGATGCAGTT GGTGCATGAA 
GATATTGAAA AGGAACATCA TTCCATCCAG AACAACGATG TGGTGGTTTT TTTTCAGGTT 
GCACAGTTTG CTATTTCTTT TCAGTATCAC AAGTTTTCAA CTTCAAAGAT AATTGAAGCT 
GAGACTGATG AGGCTCAAAC AGAACATGCT GATTCCACAT TTTTCCAAGG TAACATGTGC 
GGTCCAATAG CAGCAACAAT GAACGAGGCA ATGTTTCAGT TAGTTGTTTC CAAATGGCGT 
TATGCATTTG AAGGCTTAAA GGAAACTAAT GACTTTAAGT TTCTGTCTGC TGCTGGCTCT 
CTGATGAAAA ATATGATTTG CATGCTAGAT TTGGTGCTAA AATTATTGCC AGAAGATTCA 
AAGGAACCTC AAACAGCTCG TATTCTACTT TACAAACTAT TTTACGATCA GACAGATCAA 
GGGATGACTC AATTCCTTTT AAATTTGCTC AAGTCATTCA ACACTCACAA ACAACCCAAA 
AGTGATCTTG CAGATTTAGT TGAGATGGTC TACAAGGTTG TACAGCTTAT GGAGAACCTC 
CAAGCACGTG GAACACTTCG GGTTTCCAAA AAATCAAGGA GGGGAAGAAA GGCAAAGTCA 
GCAAATAATG GGGACAATAA ACAGTCAGAA GATCAGGGAG CTGAGAATAA GACGGCCATA 
ACCCATAATG AGCAATCAAC AGATGTTGAT GTTTGTGAAA ATAGTAACTT AAAAACGAGT 
CCTGATTGTA AAGAAGAGAT ATCTGTTACT GCTAATGCTG ACGAACCTGA ACTGTTAGAC 
TTAAACTCGG GAAGTTTTGA AGGCAGTATG CCACAGAGAG AGAACAAAAA TTTGAATGAT 
GGTTACAGCA CAGCCGACTC TTCTAGTGAT GAACAGAAAA ATAGAATTGT GGAGGTTGAT 
CTTAAAGTGT CAAGTCTAGT ATCTACTTTT GCCAATAACA ACATTATTCA GAAGATATGC 
TGGTTGCTTA AGTTTTACAA GAGCAATGCT ACTAGTACAA ACCATTACAT AATATGTATT 
TTACGGAAGA TCACTGAGGA ATTAGAGCTT TCTCCAATGC TGTACCAGTT ATCCGTCCTT 
CCCACGTTTT ATGATATTCT ATCTGAACAG AAGTCAAGTC CATGCAAGGA GCATGCTACT 
ATAGTTGATT TCCTGACGAG TCTGGTGAGA AAGATGCTAC GAAAGATAAA GAATCAGCCA 
CTCCTTTTTG TTGAAATCCT GTTTTGGAAG ACCCGAAAAG AATGCCATTA TATTGATGCT 
GAGTACTTAG TGCATGAGCT TGGCTGCTGG AAGAAAGAAA GTAGGGAGGA AAATTTTACC 
GGTGGTGATG AAAATGGCTC GTTGACAGGC AAGCATTGGA CTCCCAGAAG CATAGCAGAT 
GCACTTGGTG AAGATGAAGC TGATGTTGTG CTCACCAATA ACGGGTTTGG ATTTCATAGT 
GAAGCAAAGT CCGACGAAGT CAAGAAAGGC CTTGAGTCTA CCAACTTGGA TGATGAGATG 
GACAGAAAAG AACACAATGA AAATGAGCTG TCTATGGACA ATAAGCCCAA AAGGCTTCCT 
AAAAGAAAGA GACTTGTTCT TGATGCGGCA CTGGAGACGG AAATTAAAGA TCTTTATGAG 
AAATTCAAGG AGGACAGGAA CTGTAGCAAA CTTATTGCGG AGAATCTTGA TAATGATGTC 
AAAGTTTCGC CTGCCCAGGT TTCCAATAAG CTTAGACAGA TGGGTTTGAA AGTTGTACAA 
AGAAAGAAGA GGCAGTATGC TGATGAAGGC TTTTCTGCTA TTTCTGAAAA CCTTGAAGGA 
GAAAGCAATG GGGAAGAACT ACGTAACTCA AATGTTTTCG GGAAAAGTTC ATTGAATCAA 
CCTTCGCTCA CTAGAAAAAG AATTCTTGCT TTTGATAAAG AACACGAAGA GAAGATTAGA 
GCTTTATATG AGCAGTTTAA AGACCATAAG AGATGTAGTT CTATGATTGC AAATGCGCTG 
GATGCTGATA ACAAATTCAC TCCTGCCCAA ATTTCCCGAA AACTCAAGCA GTTGGGTTTG 
TATATTTCTC GTAAGAGAAA GTCAAGTGAT GGGGATCGTA ATGAATCTGC TATTGATAAG 
GAATCTGAAA GTGATGATGA AACATTGCTC TCATTAATAA ATAGAAAAAA AGGGAAGCAT 
CTTCCAAAGT CAACTGAAAC GCCCAGTTCC ATCTCAACAC AGAGCATATT AATTGATGAG 
GAATCTGAAG GTGTAGCTGA TGGAAGGTCC ATGCAATTGG AGGATAGAAA CCAAGCCAGT 
AGCTTGGAAA CTATGGGGGT TGGTGGACCG CCTTCAGATG ATGTTGACTT GAATGACTTT 
ACGGAAAATC AAGGGAAGGA TGCTGAAGCT GGCCGTGTCA ATATGGATGA TTTGATGCAA 
AAGGCCATGG AAGATGAGTT TGCAGATTCA GACAATGAAG TGTCTCCCAG TGTGTATCGT 
ACAACGGGAA GAAAGTTTAG GATAGTTGAT CTTGAGGATG AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011654633.1
CDS	89..3676
Translation	MEIDGLCVICAGIGIVEEDDYGNRIGYSKNEFCLDNLKDLLRFLRRDDPQTRDVFKHVCKWNIVGKDLIPIIEYCQDDRNAVLNAVKILVFLTMPIEPTSSDIAQQIEYLWGLKSLITCSNVVAIIVSLLESPLENLDCGTFSEDDWKLLQLVITLFRNVLAIQEISLQQKADGSACQLILLRDKFLEVLFRENVMDIILVMTQHIDGSCSHLRQDKLVFLEIFYYIFMGQEPELIAKVPQNSSEENVETVSSVNSLKSMMEEDRRKFSRLQNLNRHSQFSGTFTRQTLDGSKLVLKGKPSLSSSTSHKPPKVCRGPIKKIAWDLGRLTSKNSKLLQLLHDFINQFLSGGYNALMQLVHEDIEKEHHSIQNNDVVVFFQVAQFAISFQYHKFSTSKIIEAETDEAQTEHADSTFFQGNMCGPIAATMNEAMFQLVVSKWRYAFEGLKETNDFKFLSAAGSLMKNMICMLDLVLKLLPEDSKEPQTARILLYKLFYDQTDQGMTQFLLNLLKSFNTHKQPKSDLADLVEMVYKVVQLMENLQARGTLRVSKKSRRGRKAKSANNGDNKQSEDQGAENKTAITHNEQSTDVDVCENSNLKTSPDCKEEISVTANADEPELLDLNSGSFEGSMPQRENKNLNDGYSTADSSSDEQKNRIVEVDLKVSSLVSTFANNNIIQKICWLLKFYKSNATSTNHYIICILRKITEELELSPMLYQLSVLPTFYDILSEQKSSPCKEHATIVDFLTSLVRKMLRKIKNQPLLFVEILFWKTRKECHYIDAEYLVHELGCWKKESREENFTGGDENGSLTGKHWTPRSIADALGEDEADVVLTNNGFGFHSEAKSDEVKKGLESTNLDDEMDRKEHNENELSMDNKPKRLPKRKRLVLDAALETEIKDLYEKFKEDRNCSKLIAENLDNDVKVSPAQVSNKLRQMGLKVVQRKKRQYADEGFSAISENLEGESNGEELRNSNVFGKSSLNQPSLTRKRILAFDKEHEEKIRALYEQFKDHKRCSSMIANALDADNKFTPAQISRKLKQLGLYISRKRKSSDGDRNESAIDKESESDDETLLSLINRKKGKHLPKSTETPSSISTQSILIDEESEGVADGRSMQLEDRNQASSLETMGVGGPPSDDVDLNDFTENQGKDAEAGRVNMDDLMQKAMEDEFADSDNEVSPSVYRTTGRKFRIVDLEDEE

Target ORF information:

RefSeq Version	XM_011656331.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus protein timeless homolog (LOC101218315), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011656331.1

ORF Insert Sequence:

ATGGAGATAG ATGGGCTATG TGTTATTTGC GCTGGCATTG GAATCGTCGA GGAGGATGAC 
TACGGCAATC GGATCGGTTA CTCCAAAAAC GAGTTCTGTT TAGATAATTT GAAGGATTTG 
CTTAGGTTCC TGAGGAGAGA TGACCCGCAG ACTCGCGATG TTTTTAAGCA CGTTTGTAAA 
TGGAACATTG TGGGTAAAGA TTTGATTCCC ATCATTGAGT ACTGTCAAGA TGATCGTAAT 
GCAGTTTTAA ATGCAGTGAA GATTTTGGTG TTCCTCACAA TGCCTATCGA ACCCACGTCA 
AGTGACATTG CACAACAGAT CGAGTATCTA TGGGGATTGA AGTCTTTAAT CACATGCAGT 
AATGTTGTTG CCATAATTGT TTCACTTCTG GAGAGTCCGC TGGAAAATCT GGATTGCGGA 
ACATTCTCAG AAGATGACTG GAAATTGTTG CAGTTGGTTA TAACACTCTT CCGAAATGTT 
TTGGCTATTC AAGAAATCTC ATTGCAGCAA AAGGCTGATG GATCAGCTTG TCAGTTAATA 
TTGCTGAGAG ACAAATTTTT GGAAGTTTTA TTCCGGGAGA ATGTTATGGA CATAATATTG 
GTGATGACGC AGCACATTGA TGGGTCTTGC AGCCATCTCC GTCAAGACAA ATTAGTCTTT 
TTGGAAATAT TTTACTACAT ATTTATGGGC CAAGAGCCTG AGTTAATCGC GAAAGTGCCT 
CAAAACAGCA GTGAGGAAAA TGTAGAAACG GTATCTTCTG TCAATAGTCT GAAATCTATG 
ATGGAGGAAG ATAGAAGAAA GTTTTCTAGA TTACAAAATT TGAACCGACA TTCGCAGTTT 
AGTGGAACTT TTACTCGGCA AACCTTGGAT GGTTCAAAGT TAGTACTCAA AGGAAAACCA 
TCTTTGAGTT CTTCTACCAG CCATAAGCCA CCTAAAGTTT GTCGAGGTCC AATTAAAAAA 
ATTGCATGGG ACCTTGGACG ATTAACATCG AAAAACAGCA AACTTCTGCA GTTACTTCAT 
GATTTTATTA ATCAGTTTCT TTCAGGCGGC TACAATGCGC TGATGCAGTT GGTGCATGAA 
GATATTGAAA AGGAACATCA TTCCATCCAG AACAACGATG TGGTGGTTTT TTTTCAGGTT 
GCACAGTTTG CTATTTCTTT TCAGTATCAC AAGTTTTCAA CTTCAAAGAT AATTGAAGCT 
GAGACTGATG AGGCTCAAAC AGAACATGCT GATTCCACAT TTTTCCAAGG TAACATGTGC 
GGTCCAATAG CAGCAACAAT GAACGAGGCA ATGTTTCAGT TAGTTGTTTC CAAATGGCGT 
TATGCATTTG AAGGCTTAAA GGAAACTAAT GACTTTAAGT TTCTGTCTGC TGCTGGCTCT 
CTGATGAAAA ATATGATTTG CATGCTAGAT TTGGTGCTAA AATTATTGCC AGAAGATTCA 
AAGGAACCTC AAACAGCTCG TATTCTACTT TACAAACTAT TTTACGATCA GACAGATCAA 
GGGATGACTC AATTCCTTTT AAATTTGCTC AAGTCATTCA ACACTCACAA ACAACCCAAA 
AGTGATCTTG CAGATTTAGT TGAGATGGTC TACAAGGTTG TACAGCTTAT GGAGAACCTC 
CAAGCACGTG GAACACTTCG GGTTTCCAAA AAATCAAGGA GGGGAAGAAA GGCAAAGTCA 
GCAAATAATG GGGACAATAA ACAGTCAGAA GATCAGGGAG CTGAGAATAA GACGGCCATA 
ACCCATAATG AGCAATCAAC AGATGTTGAT GTTTGTGAAA ATAGTAACTT AAAAACGAGT 
CCTGATTGTA AAGAAGAGAT ATCTGTTACT GCTAATGCTG ACGAACCTGA ACTGTTAGAC 
TTAAACTCGG GAAGTTTTGA AGGCAGTATG CCACAGAGAG AGAACAAAAA TTTGAATGAT 
GGTTACAGCA CAGCCGACTC TTCTAGTGAT GAACAGAAAA ATAGAATTGT GGAGGTTGAT 
CTTAAAGTGT CAAGTCTAGT ATCTACTTTT GCCAATAACA ACATTATTCA GAAGATATGC 
TGGTTGCTTA AGTTTTACAA GAGCAATGCT ACTAGTACAA ACCATTACAT AATATGTATT 
TTACGGAAGA TCACTGAGGA ATTAGAGCTT TCTCCAATGC TGTACCAGTT ATCCGTCCTT 
CCCACGTTTT ATGATATTCT ATCTGAACAG AAGTCAAGTC CATGCAAGGA GCATGCTACT 
ATAGTTGATT TCCTGACGAG TCTGGTGAGA AAGATGCTAC GAAAGATAAA GAATCAGCCA 
CTCCTTTTTG TTGAAATCCT GTTTTGGAAG ACCCGAAAAG AATGCCATTA TATTGATGCT 
GAGTACTTAG TGCATGAGCT TGGCTGCTGG AAGAAAGAAA GTAGGGAGGA AAATTTTACC 
GGTGGTGATG AAAATGGCTC GTTGACAGGC AAGCATTGGA CTCCCAGAAG CATAGCAGAT 
GCACTTGGTG AAGATGAAGC TGATGTTGTG CTCACCAATA ACGGGTTTGG ATTTCATAGT 
GAAGCAAAGT CCGACGAAGT CAAGAAAGGC CTTGAGTCTA CCAACTTGGA TGATGAGATG 
GACAGAAAAG AACACAATGA AAATGAGCTG TCTATGGACA ATAAGCCCAA AAGGCTTCCT 
AAAAGAAAGA GACTTGTTCT TGATGCGGCA CTGGAGACGG AAATTAAAGA TCTTTATGAG 
AAATTCAAGG AGGACAGGAA CTGTAGCAAA CTTATTGCGG AGAATCTTGA TAATGATGTC 
AAAGTTTCGC CTGCCCAGGT TTCCAATAAG CTTAGACAGA TGGGTTTGAA AGTTGTACAA 
AGAAAGAAGA GGCAGTATGC TGATGAAGGC TTTTCTGCTA TTTCTGAAAA CCTTGAAGGA 
GAAAGCAATG GGGAAGAACT ACGTAACTCA AATGTTTTCG GGAAAAGTTC ATTGAATCAA 
CCTTCGCTCA CTAGAAAAAG AATTCTTGCT TTTGATAAAG AACACGAAGA GAAGATTAGA 
GCTTTATATG AGCAGTTTAA AGACCATAAG AGATGTAGTT CTATGATTGC AAATGCGCTG 
GATGCTGATA ACAAATTCAC TCCTGCCCAA ATTTCCCGAA AACTCAAGCA GTTGGGTTTG 
TATATTTCTC GTAAGAGAAA GTCAAGTGAT GGGGATCGTA ATGAATCTGC TATTGATAAG 
GAATCTGAAA GTGATGATGA AACATTGCTC TCATTAATAA ATAGAAAAAA AGGGAAGCAT 
CTTCCAAAGT CAACTGAAAC GCCCAGTTCC ATCTCAACAC AGAGCATATT AATTGATGAG 
GAATCTGAAG GTGTAGCTGA TGGAAGGTCC ATGCAATTGG AGGATAGAAA CCAAGCCAGT 
AGCTTGGAAA CTATGGGGGT TGGTGGACCG CCTTCAGATG ATGTTGACTT GAATGACTTT 
ACGGAAAATC AAGGGAAGGA TGCTGAAGCT GGCCGTGTCA ATATGGATGA TTTGATGCAA 
AAGGCCATGG AAGATGAGTT TGCAGATTCA GACAATGAAG TGTCTCCCAG TGTGTATCGT 
ACAACGGGAA GAAAGTTTAG GATAGTTGAT CTTGAGGATG AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101218315 ( XM_011656331.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011654633.1 Cucumis sativus protein timeless homolog (LOC101218315), mRNA.

Price & Availability↑

CloneID	OCu65770
Clone ID Related Accession (Same CDS sequence)	XM_011656331.1 , XM_011656331.2
Accession Version	XM_011656331.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	protein timeless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026659.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011656331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATAG ATGGGCTATG TGTTATTTGC GCTGGCATTG GAATCGTCGA GGAGGATGAC 
TACGGCAATC GGATCGGTTA CTCCAAAAAC GAGTTCTGTT TAGATAATTT GAAGGATTTG 
CTTAGGTTCC TGAGGAGAGA TGACCCGCAG ACTCGCGATG TTTTTAAGCA CGTTTGTAAA 
TGGAACATTG TGGGTAAAGA TTTGATTCCC ATCATTGAGT ACTGTCAAGA TGATCGTAAT 
GCAGTTTTAA ATGCAGTGAA GATTTTGGTG TTCCTCACAA TGCCTATCGA ACCCACGTCA 
AGTGACATTG CACAACAGAT CGAGTATCTA TGGGGATTGA AGTCTTTAAT CACATGCAGT 
AATGTTGTTG CCATAATTGT TTCACTTCTG GAGAGTCCGC TGGAAAATCT GGATTGCGGA 
ACATTCTCAG AAGATGACTG GAAATTGTTG CAGTTGGTTA TAACACTCTT CCGAAATGTT 
TTGGCTATTC AAGAAATCTC ATTGCAGCAA AAGGCTGATG GATCAGCTTG TCAGTTAATA 
TTGCTGAGAG ACAAATTTTT GGAAGTTTTA TTCCGGGAGA ATGTTATGGA CATAATATTG 
GTGATGACGC AGCACATTGA TGGGTCTTGC AGCCATCTCC GTCAAGACAA ATTAGTCTTT 
TTGGAAATAT TTTACTACAT ATTTATGGGC CAAGAGCCTG AGTTAATCGC GAAAGTGCCT 
CAAAACAGCA GTGAGGAAAA TGTAGAAACG GTATCTTCTG TCAATAGTCT GAAATCTATG 
ATGGAGGAAG ATAGAAGAAA GTTTTCTAGA TTACAAAATT TGAACCGACA TTCGCAGTTT 
AGTGGAACTT TTACTCGGCA AACCTTGGAT GGTTCAAAGT TAGTACTCAA AGGAAAACCA 
TCTTTGAGTT CTTCTACCAG CCATAAGCCA CCTAAAGTTT GTCGAGGTCC AATTAAAAAA 
ATTGCATGGG ACCTTGGACG ATTAACATCG AAAAACAGCA AACTTCTGCA GTTACTTCAT 
GATTTTATTA ATCAGTTTCT TTCAGGCGGC TACAATGCGC TGATGCAGTT GGTGCATGAA 
GATATTGAAA AGGAACATCA TTCCATCCAG AACAACGATG TGGTGGTTTT TTTTCAGGTT 
GCACAGTTTG CTATTTCTTT TCAGTATCAC AAGTTTTCAA CTTCAAAGAT AATTGAAGCT 
GAGACTGATG AGGCTCAAAC AGAACATGCT GATTCCACAT TTTTCCAAGG TAACATGTGC 
GGTCCAATAG CAGCAACAAT GAACGAGGCA ATGTTTCAGT TAGTTGTTTC CAAATGGCGT 
TATGCATTTG AAGGCTTAAA GGAAACTAAT GACTTTAAGT TTCTGTCTGC TGCTGGCTCT 
CTGATGAAAA ATATGATTTG CATGCTAGAT TTGGTGCTAA AATTATTGCC AGAAGATTCA 
AAGGAACCTC AAACAGCTCG TATTCTACTT TACAAACTAT TTTACGATCA GACAGATCAA 
GGGATGACTC AATTCCTTTT AAATTTGCTC AAGTCATTCA ACACTCACAA ACAACCCAAA 
AGTGATCTTG CAGATTTAGT TGAGATGGTC TACAAGGTTG TACAGCTTAT GGAGAACCTC 
CAAGCACGTG GAACACTTCG GGTTTCCAAA AAATCAAGGA GGGGAAGAAA GGCAAAGTCA 
GCAAATAATG GGGACAATAA ACAGTCAGAA GATCAGGGAG CTGAGAATAA GACGGCCATA 
ACCCATAATG AGCAATCAAC AGATGTTGAT GTTTGTGAAA ATAGTAACTT AAAAACGAGT 
CCTGATTGTA AAGAAGAGAT ATCTGTTACT GCTAATGCTG ACGAACCTGA ACTGTTAGAC 
TTAAACTCGG GAAGTTTTGA AGGCAGTATG CCACAGAGAG AGAACAAAAA TTTGAATGAT 
GGTTACAGCA CAGCCGACTC TTCTAGTGAT GAACAGAAAA ATAGAATTGT GGAGGTTGAT 
CTTAAAGTGT CAAGTCTAGT ATCTACTTTT GCCAATAACA ACATTATTCA GAAGATATGC 
TGGTTGCTTA AGTTTTACAA GAGCAATGCT ACTAGTACAA ACCATTACAT AATATGTATT 
TTACGGAAGA TCACTGAGGA ATTAGAGCTT TCTCCAATGC TGTACCAGTT ATCCGTCCTT 
CCCACGTTTT ATGATATTCT ATCTGAACAG AAGTCAAGTC CATGCAAGGA GCATGCTACT 
ATAGTTGATT TCCTGACGAG TCTGGTGAGA AAGATGCTAC GAAAGATAAA GAATCAGCCA 
CTCCTTTTTG TTGAAATCCT GTTTTGGAAG ACCCGAAAAG AATGCCATTA TATTGATGCT 
GAGTACTTAG TGCATGAGCT TGGCTGCTGG AAGAAAGAAA GTAGGGAGGA AAATTTTACC 
GGTGGTGATG AAAATGGCTC GTTGACAGGC AAGCATTGGA CTCCCAGAAG CATAGCAGAT 
GCACTTGGTG AAGATGAAGC TGATGTTGTG CTCACCAATA ACGGGTTTGG ATTTCATAGT 
GAAGCAAAGT CCGACGAAGT CAAGAAAGGC CTTGAGTCTA CCAACTTGGA TGATGAGATG 
GACAGAAAAG AACACAATGA AAATGAGCTG TCTATGGACA ATAAGCCCAA AAGGCTTCCT 
AAAAGAAAGA GACTTGTTCT TGATGCGGCA CTGGAGACGG AAATTAAAGA TCTTTATGAG 
AAATTCAAGG AGGACAGGAA CTGTAGCAAA CTTATTGCGG AGAATCTTGA TAATGATGTC 
AAAGTTTCGC CTGCCCAGGT TTCCAATAAG CTTAGACAGA TGGGTTTGAA AGTTGTACAA 
AGAAAGAAGA GGCAGTATGC TGATGAAGGC TTTTCTGCTA TTTCTGAAAA CCTTGAAGGA 
GAAAGCAATG GGGAAGAACT ACGTAACTCA AATGTTTTCG GGAAAAGTTC ATTGAATCAA 
CCTTCGCTCA CTAGAAAAAG AATTCTTGCT TTTGATAAAG AACACGAAGA GAAGATTAGA 
GCTTTATATG AGCAGTTTAA AGACCATAAG AGATGTAGTT CTATGATTGC AAATGCGCTG 
GATGCTGATA ACAAATTCAC TCCTGCCCAA ATTTCCCGAA AACTCAAGCA GTTGGGTTTG 
TATATTTCTC GTAAGAGAAA GTCAAGTGAT GGGGATCGTA ATGAATCTGC TATTGATAAG 
GAATCTGAAA GTGATGATGA AACATTGCTC TCATTAATAA ATAGAAAAAA AGGGAAGCAT 
CTTCCAAAGT CAACTGAAAC GCCCAGTTCC ATCTCAACAC AGAGCATATT AATTGATGAG 
GAATCTGAAG GTGTAGCTGA TGGAAGGTCC ATGCAATTGG AGGATAGAAA CCAAGCCAGT 
AGCTTGGAAA CTATGGGGGT TGGTGGACCG CCTTCAGATG ATGTTGACTT GAATGACTTT 
ACGGAAAATC AAGGGAAGGA TGCTGAAGCT GGCCGTGTCA ATATGGATGA TTTGATGCAA 
AAGGCCATGG AAGATGAGTT TGCAGATTCA GACAATGAAG TGTCTCCCAG TGTGTATCGT 
ACAACGGGAA GAAAGTTTAG GATAGTTGAT CTTGAGGATG AAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011654633.1
CDS	130..3717
Translation	MEIDGLCVICAGIGIVEEDDYGNRIGYSKNEFCLDNLKDLLRFLRRDDPQTRDVFKHVCKWNIVGKDLIPIIEYCQDDRNAVLNAVKILVFLTMPIEPTSSDIAQQIEYLWGLKSLITCSNVVAIIVSLLESPLENLDCGTFSEDDWKLLQLVITLFRNVLAIQEISLQQKADGSACQLILLRDKFLEVLFRENVMDIILVMTQHIDGSCSHLRQDKLVFLEIFYYIFMGQEPELIAKVPQNSSEENVETVSSVNSLKSMMEEDRRKFSRLQNLNRHSQFSGTFTRQTLDGSKLVLKGKPSLSSSTSHKPPKVCRGPIKKIAWDLGRLTSKNSKLLQLLHDFINQFLSGGYNALMQLVHEDIEKEHHSIQNNDVVVFFQVAQFAISFQYHKFSTSKIIEAETDEAQTEHADSTFFQGNMCGPIAATMNEAMFQLVVSKWRYAFEGLKETNDFKFLSAAGSLMKNMICMLDLVLKLLPEDSKEPQTARILLYKLFYDQTDQGMTQFLLNLLKSFNTHKQPKSDLADLVEMVYKVVQLMENLQARGTLRVSKKSRRGRKAKSANNGDNKQSEDQGAENKTAITHNEQSTDVDVCENSNLKTSPDCKEEISVTANADEPELLDLNSGSFEGSMPQRENKNLNDGYSTADSSSDEQKNRIVEVDLKVSSLVSTFANNNIIQKICWLLKFYKSNATSTNHYIICILRKITEELELSPMLYQLSVLPTFYDILSEQKSSPCKEHATIVDFLTSLVRKMLRKIKNQPLLFVEILFWKTRKECHYIDAEYLVHELGCWKKESREENFTGGDENGSLTGKHWTPRSIADALGEDEADVVLTNNGFGFHSEAKSDEVKKGLESTNLDDEMDRKEHNENELSMDNKPKRLPKRKRLVLDAALETEIKDLYEKFKEDRNCSKLIAENLDNDVKVSPAQVSNKLRQMGLKVVQRKKRQYADEGFSAISENLEGESNGEELRNSNVFGKSSLNQPSLTRKRILAFDKEHEEKIRALYEQFKDHKRCSSMIANALDADNKFTPAQISRKLKQLGLYISRKRKSSDGDRNESAIDKESESDDETLLSLINRKKGKHLPKSTETPSSISTQSILIDEESEGVADGRSMQLEDRNQASSLETMGVGGPPSDDVDLNDFTENQGKDAEAGRVNMDDLMQKAMEDEFADSDNEVSPSVYRTTGRKFRIVDLEDEE

Target ORF information:

RefSeq Version	XM_011656331.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus protein timeless homolog (LOC101218315), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011656331.2

ORF Insert Sequence:

ATGGAGATAG ATGGGCTATG TGTTATTTGC GCTGGCATTG GAATCGTCGA GGAGGATGAC 
TACGGCAATC GGATCGGTTA CTCCAAAAAC GAGTTCTGTT TAGATAATTT GAAGGATTTG 
CTTAGGTTCC TGAGGAGAGA TGACCCGCAG ACTCGCGATG TTTTTAAGCA CGTTTGTAAA 
TGGAACATTG TGGGTAAAGA TTTGATTCCC ATCATTGAGT ACTGTCAAGA TGATCGTAAT 
GCAGTTTTAA ATGCAGTGAA GATTTTGGTG TTCCTCACAA TGCCTATCGA ACCCACGTCA 
AGTGACATTG CACAACAGAT CGAGTATCTA TGGGGATTGA AGTCTTTAAT CACATGCAGT 
AATGTTGTTG CCATAATTGT TTCACTTCTG GAGAGTCCGC TGGAAAATCT GGATTGCGGA 
ACATTCTCAG AAGATGACTG GAAATTGTTG CAGTTGGTTA TAACACTCTT CCGAAATGTT 
TTGGCTATTC AAGAAATCTC ATTGCAGCAA AAGGCTGATG GATCAGCTTG TCAGTTAATA 
TTGCTGAGAG ACAAATTTTT GGAAGTTTTA TTCCGGGAGA ATGTTATGGA CATAATATTG 
GTGATGACGC AGCACATTGA TGGGTCTTGC AGCCATCTCC GTCAAGACAA ATTAGTCTTT 
TTGGAAATAT TTTACTACAT ATTTATGGGC CAAGAGCCTG AGTTAATCGC GAAAGTGCCT 
CAAAACAGCA GTGAGGAAAA TGTAGAAACG GTATCTTCTG TCAATAGTCT GAAATCTATG 
ATGGAGGAAG ATAGAAGAAA GTTTTCTAGA TTACAAAATT TGAACCGACA TTCGCAGTTT 
AGTGGAACTT TTACTCGGCA AACCTTGGAT GGTTCAAAGT TAGTACTCAA AGGAAAACCA 
TCTTTGAGTT CTTCTACCAG CCATAAGCCA CCTAAAGTTT GTCGAGGTCC AATTAAAAAA 
ATTGCATGGG ACCTTGGACG ATTAACATCG AAAAACAGCA AACTTCTGCA GTTACTTCAT 
GATTTTATTA ATCAGTTTCT TTCAGGCGGC TACAATGCGC TGATGCAGTT GGTGCATGAA 
GATATTGAAA AGGAACATCA TTCCATCCAG AACAACGATG TGGTGGTTTT TTTTCAGGTT 
GCACAGTTTG CTATTTCTTT TCAGTATCAC AAGTTTTCAA CTTCAAAGAT AATTGAAGCT 
GAGACTGATG AGGCTCAAAC AGAACATGCT GATTCCACAT TTTTCCAAGG TAACATGTGC 
GGTCCAATAG CAGCAACAAT GAACGAGGCA ATGTTTCAGT TAGTTGTTTC CAAATGGCGT 
TATGCATTTG AAGGCTTAAA GGAAACTAAT GACTTTAAGT TTCTGTCTGC TGCTGGCTCT 
CTGATGAAAA ATATGATTTG CATGCTAGAT TTGGTGCTAA AATTATTGCC AGAAGATTCA 
AAGGAACCTC AAACAGCTCG TATTCTACTT TACAAACTAT TTTACGATCA GACAGATCAA 
GGGATGACTC AATTCCTTTT AAATTTGCTC AAGTCATTCA ACACTCACAA ACAACCCAAA 
AGTGATCTTG CAGATTTAGT TGAGATGGTC TACAAGGTTG TACAGCTTAT GGAGAACCTC 
CAAGCACGTG GAACACTTCG GGTTTCCAAA AAATCAAGGA GGGGAAGAAA GGCAAAGTCA 
GCAAATAATG GGGACAATAA ACAGTCAGAA GATCAGGGAG CTGAGAATAA GACGGCCATA 
ACCCATAATG AGCAATCAAC AGATGTTGAT GTTTGTGAAA ATAGTAACTT AAAAACGAGT 
CCTGATTGTA AAGAAGAGAT ATCTGTTACT GCTAATGCTG ACGAACCTGA ACTGTTAGAC 
TTAAACTCGG GAAGTTTTGA AGGCAGTATG CCACAGAGAG AGAACAAAAA TTTGAATGAT 
GGTTACAGCA CAGCCGACTC TTCTAGTGAT GAACAGAAAA ATAGAATTGT GGAGGTTGAT 
CTTAAAGTGT CAAGTCTAGT ATCTACTTTT GCCAATAACA ACATTATTCA GAAGATATGC 
TGGTTGCTTA AGTTTTACAA GAGCAATGCT ACTAGTACAA ACCATTACAT AATATGTATT 
TTACGGAAGA TCACTGAGGA ATTAGAGCTT TCTCCAATGC TGTACCAGTT ATCCGTCCTT 
CCCACGTTTT ATGATATTCT ATCTGAACAG AAGTCAAGTC CATGCAAGGA GCATGCTACT 
ATAGTTGATT TCCTGACGAG TCTGGTGAGA AAGATGCTAC GAAAGATAAA GAATCAGCCA 
CTCCTTTTTG TTGAAATCCT GTTTTGGAAG ACCCGAAAAG AATGCCATTA TATTGATGCT 
GAGTACTTAG TGCATGAGCT TGGCTGCTGG AAGAAAGAAA GTAGGGAGGA AAATTTTACC 
GGTGGTGATG AAAATGGCTC GTTGACAGGC AAGCATTGGA CTCCCAGAAG CATAGCAGAT 
GCACTTGGTG AAGATGAAGC TGATGTTGTG CTCACCAATA ACGGGTTTGG ATTTCATAGT 
GAAGCAAAGT CCGACGAAGT CAAGAAAGGC CTTGAGTCTA CCAACTTGGA TGATGAGATG 
GACAGAAAAG AACACAATGA AAATGAGCTG TCTATGGACA ATAAGCCCAA AAGGCTTCCT 
AAAAGAAAGA GACTTGTTCT TGATGCGGCA CTGGAGACGG AAATTAAAGA TCTTTATGAG 
AAATTCAAGG AGGACAGGAA CTGTAGCAAA CTTATTGCGG AGAATCTTGA TAATGATGTC 
AAAGTTTCGC CTGCCCAGGT TTCCAATAAG CTTAGACAGA TGGGTTTGAA AGTTGTACAA 
AGAAAGAAGA GGCAGTATGC TGATGAAGGC TTTTCTGCTA TTTCTGAAAA CCTTGAAGGA 
GAAAGCAATG GGGAAGAACT ACGTAACTCA AATGTTTTCG GGAAAAGTTC ATTGAATCAA 
CCTTCGCTCA CTAGAAAAAG AATTCTTGCT TTTGATAAAG AACACGAAGA GAAGATTAGA 
GCTTTATATG AGCAGTTTAA AGACCATAAG AGATGTAGTT CTATGATTGC AAATGCGCTG 
GATGCTGATA ACAAATTCAC TCCTGCCCAA ATTTCCCGAA AACTCAAGCA GTTGGGTTTG 
TATATTTCTC GTAAGAGAAA GTCAAGTGAT GGGGATCGTA ATGAATCTGC TATTGATAAG 
GAATCTGAAA GTGATGATGA AACATTGCTC TCATTAATAA ATAGAAAAAA AGGGAAGCAT 
CTTCCAAAGT CAACTGAAAC GCCCAGTTCC ATCTCAACAC AGAGCATATT AATTGATGAG 
GAATCTGAAG GTGTAGCTGA TGGAAGGTCC ATGCAATTGG AGGATAGAAA CCAAGCCAGT 
AGCTTGGAAA CTATGGGGGT TGGTGGACCG CCTTCAGATG ATGTTGACTT GAATGACTTT 
ACGGAAAATC AAGGGAAGGA TGCTGAAGCT GGCCGTGTCA ATATGGATGA TTTGATGCAA 
AAGGCCATGG AAGATGAGTT TGCAGATTCA GACAATGAAG TGTCTCCCAG TGTGTATCGT 
ACAACGGGAA GAAAGTTTAG GATAGTTGAT CTTGAGGATG AAGAATGA