LOC101216047 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101216047
Entrez Gene ID	101216047
Full Name	probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011652491.2	XP_011650793.2	probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial
XM_011652491.1	XP_011650793.1	probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC101216047 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101216047 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu148046	XM_011652491.2 Latest version!	Cucumis sativus probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial (LOC101216047), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCu59259	XM_011652491.1 Latest version!	Cucumis sativus probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial (LOC101216047), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101216047 ( XM_011652491.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011650793.2 Cucumis sativus probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial (LOC101216047), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCu148046
Clone ID Related Accession (Same CDS sequence)	XM_011652491.2
Accession Version	XM_011652491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026657.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_011652491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAT CCACCATTGA GGCCCTTCAG CATCGCTGCT GCTGCTCCGT GGGAATGGCG 
TTTTCTTCCA CACTTTCCCG CTTAAACCTT ATCCCAAAAC CTTCACTTCC TTACGCGCTT 
ACTACCTTCA AAGTTTTGCA CTTCCACAAG TTATTACGAA CCGATTGGAT TGGACCAGGT 
CTCTCATCTA ATGCTTCAAC TCCCCGATTT TCTGCTTTGA AAATCACAAG CTCTGCTGGA 
TTTCACACTC AGATTTCAAG GCCACAAGAC GATGATTTGA TTGTTCTCGG CATTGAAACG 
AGCTGCGATG ATACCGCCGC TGCCATTGTG AAGGGCAATG GCGAAATCCT TAGCCAAGTT 
ATAGCTTCTC AGGCAGAGCT GCTTGCTCGC TATGGAGGCG TTGCTCCTAA AATGGCAGAA 
GAAGCGCACG CACAAGTGAT TGACAAGGTG GTGCAGGAAA CAATGGAAAA GGCTAACTTA 
ACAGAAAGGG ATTTATCTGC AGTTGCTGTG ACCATTGGCC CTGGTTTGAG CCTCTGCCTC 
CGTGTTGGAG TGCAGAAGGC ACGAAAAATT GCTGGTAGCT CTAGTCTACC AATTGTTGGG 
GTGCATCACA TGGAAGCTCA TGCTCTGGTA GCTAGATTAG TTGAACGGGA ACTGCAATTT 
CCTTTCATGG CACTGCTCAT TTCTGGGGGA CACAATTTAC TTGTTCTCGC ACAAGATCTT 
GGCCAATACA TACAACTTGG AACAACAATA GACGATGCCA TTGGGGAGGC GTATGACAAG 
ACTGCCAAAT GGCTTGGTCT TGATCTGAGG AAGAGTGGTG GGCCGGCTAT AGAGGAGCTT 
GCCAGAGAGG GGGATCCAAA GTCAATAAAG TTTTCTACTC CAATGAAGCA ACATAAAGAT 
TGCAACTTCT CCTATGCGGG GCTGAAGACC CAAGTCAGGC TGGCTATTGA ATCCCGAAAC 
ATTGACACAA AAATTCCTCT GTCGTCTGCA AGCATCCAAG ACAGAAGTGT AAGGGCTGAT 
ATAGCTGCCT CCTTTCAGCG TGTTGCAGTG CTACATCTAG AGGACAAATG TGAACGAGCA 
ATTGAATGGG CGTTGAAGAT TGAACCATCC ATAAGACATT TGGTTGTCTC AGGAGGTGTT 
GCATCAAATA AGTATGTTAG GGCTCAACTT ATGGCGGTTG TCAGGAAGAA GGGCCTGCAG 
CTTGTCTGCC CTCCTCCCAG TCTCTGCACT GATAATGGTG TAATGGTGGC TTGGACGGGC 
CTCGAGCATT TTCGCATAGG GAGATTTGAT CCTCCTCCAC CAGCTGATGA ACCTGAAGAT 
ACAATGTATG ACGTGCGACC GAGATGGCCA TTAGGAGAAG AATACACCGA GGGAAGGAGT 
GCAGCTCGCT CACTAAGAAC AGCCCGAATC CATCCATCAC TTACGTCTAT CATCCAAGCA 
TCTCTACAGC AACAACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011650793.2
CDS	2..1462
Translation	MSSSTIEALQHRCCCSVGMAFSSTLSRLNLIPKPSLPYALTTFKVLHFHKLLRTDWIGPGLSSNASTPRFSALKITSSAGFHTQISRPQDDDLIVLGIETSCDDTAAAIVKGNGEILSQVIASQAELLARYGGVAPKMAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ

Target ORF information:

RefSeq Version	XM_011652491.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial (LOC101216047), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011652491.2

ORF Insert Sequence:

ATGTCTTCAT CCACCATTGA GGCCCTTCAG CATCGCTGCT GCTGCTCCGT GGGAATGGCG 
TTTTCTTCCA CACTTTCCCG CTTAAACCTT ATCCCAAAAC CTTCACTTCC TTACGCGCTT 
ACTACCTTCA AAGTTTTGCA CTTCCACAAG TTATTACGAA CCGATTGGAT TGGACCAGGT 
CTCTCATCTA ATGCTTCAAC TCCCCGATTT TCTGCTTTGA AAATCACAAG CTCTGCTGGA 
TTTCACACTC AGATTTCAAG GCCACAAGAC GATGATTTGA TTGTTCTCGG CATTGAAACG 
AGCTGCGATG ATACCGCCGC TGCCATTGTG AAGGGCAATG GCGAAATCCT TAGCCAAGTT 
ATAGCTTCTC AGGCAGAGCT GCTTGCTCGC TATGGAGGCG TTGCTCCTAA AATGGCAGAA 
GAAGCGCACG CACAAGTGAT TGACAAGGTG GTGCAGGAAA CAATGGAAAA GGCTAACTTA 
ACAGAAAGGG ATTTATCTGC AGTTGCTGTG ACCATTGGCC CTGGTTTGAG CCTCTGCCTC 
CGTGTTGGAG TGCAGAAGGC ACGAAAAATT GCTGGTAGCT CTAGTCTACC AATTGTTGGG 
GTGCATCACA TGGAAGCTCA TGCTCTGGTA GCTAGATTAG TTGAACGGGA ACTGCAATTT 
CCTTTCATGG CACTGCTCAT TTCTGGGGGA CACAATTTAC TTGTTCTCGC ACAAGATCTT 
GGCCAATACA TACAACTTGG AACAACAATA GACGATGCCA TTGGGGAGGC GTATGACAAG 
ACTGCCAAAT GGCTTGGTCT TGATCTGAGG AAGAGTGGTG GGCCGGCTAT AGAGGAGCTT 
GCCAGAGAGG GGGATCCAAA GTCAATAAAG TTTTCTACTC CAATGAAGCA ACATAAAGAT 
TGCAACTTCT CCTATGCGGG GCTGAAGACC CAAGTCAGGC TGGCTATTGA ATCCCGAAAC 
ATTGACACAA AAATTCCTCT GTCGTCTGCA AGCATCCAAG ACAGAAGTGT AAGGGCTGAT 
ATAGCTGCCT CCTTTCAGCG TGTTGCAGTG CTACATCTAG AGGACAAATG TGAACGAGCA 
ATTGAATGGG CGTTGAAGAT TGAACCATCC ATAAGACATT TGGTTGTCTC AGGAGGTGTT 
GCATCAAATA AGTATGTTAG GGCTCAACTT ATGGCGGTTG TCAGGAAGAA GGGCCTGCAG 
CTTGTCTGCC CTCCTCCCAG TCTCTGCACT GATAATGGTG TAATGGTGGC TTGGACGGGC 
CTCGAGCATT TTCGCATAGG GAGATTTGAT CCTCCTCCAC CAGCTGATGA ACCTGAAGAT 
ACAATGTATG ACGTGCGACC GAGATGGCCA TTAGGAGAAG AATACACCGA GGGAAGGAGT 
GCAGCTCGCT CACTAAGAAC AGCCCGAATC CATCCATCAC TTACGTCTAT CATCCAAGCA 
TCTCTACAGC AACAACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101216047 ( XM_011652491.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_011650793.1 Cucumis sativus probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial (LOC101216047), mRNA.

Price & Availability↑

CloneID	OCu59259
Clone ID Related Accession (Same CDS sequence)	XM_011652491.1
Accession Version	XM_011652491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953805.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTTT CTTCCACACT TTCCCGCTTA AACCTTATCC CAAAACCTTC ACTTCCTTAC 
GCGCTTACTA CCTTCAAAGT TTTGCACTTC CACAAGTTAT TACGAACCGA TTGGATTGGA 
CCAGGTCTCT CATCTAATGC TTCAACTCCC CGATTTTCTG CTTTGAAAAT CACAAGCTCT 
GCTGGATTTC ACACTCAGAT TTCAAGGCCA CAAGACGATG ATTTGATTGT TCTCGGCATT 
GAAACGAGCT GCGATGATAC CGCCGCTGCC ATTGTGAAGG GCAATGGCGA AATCCTTAGC 
CAAGTTATAG CTTCTCAGGC AGAGCTGCTT GCTCGCTATG GAGGCGTTGC TCCTAAAATG 
GCAGAAGAAG CGCACGCACA AGTGATTGAC AAGGTGGTGC AGGAAACAAT GGAAAAGGCT 
AACTTAACAG AAAGGGATTT ATCTGCAGTT GCTGTGACCA TTGGCCCTGG TTTGAGCCTC 
TGCCTCCGTG TTGGAGTGCA GAAGGCACGA AAAATTGCTG GTAGCTCTAG TCTACCAATT 
GTTGGGGTGC ATCACATGGA AGCTCATGCT CTGGTAGCTA GATTAGTTGA ACGGGAACTG 
CAATTTCCTT TCATGGCACT GCTCATTTCT GGGGGACACA ATTTACTTGT TCTCGCACAA 
GATCTTGGCC AATACATACA ACTTGGAACA ACAATAGACG ATGCCATTGG GGAGGCGTAT 
GACAAGACTG CCAAATGGCT TGGTCTTGAT CTGAGGAAGA GTGGTGGGCC GGCTATAGAG 
GAGCTTGCCA GAGAGGGGGA TCCAAAGTCA ATAAAGTTTT CTACTCCAAT GAAGCAACAT 
AAAGATTGCA ACTTCTCCTA TGCGGGGCTG AAGACCCAAG TCAGGCTGGC TATTGAATCC 
CGAAACATTG ACACAAAAAT TCCTCTGTCG TCTGCAAGCA TCCAAGACAG AAGTGTAAGG 
GCTGATATAG CTGCCTCCTT TCAGCGTGTT GCAGTGCTAC ATCTAGAGGA CAAATGTGAA 
CGAGCAATTG AATGGGCGTT GAAGATTGAA CCATCCATAA GACATTTGGT TGTCTCAGGA 
GGTGTTGCAT CAAATAAGTA TGTTAGGGCT CAACTTATGG CGGTTGTCAG GAAGAAGGGC 
CTGCAGCTTG TCTGCCCTCC TCCCAGTCTC TGCACTGATA ATGGTGTAAT GGTGGCTTGG 
ACGGGCCTCG AGCATTTTCG CATAGGGAGA TTTGATCCTC CTCCACCAGC TGATGAACCT 
GAAGATACAA TGTATGACGT GCGACCGAGA TGGCCATTAG GAGAAGAATA CACCGAGGGA 
AGGAGTGCAG CTCGCTCACT AAGAACAGCC CGAATCCATC CATCACTTAC GTCTATCATC 
CAAGCATCTC TACAGCAACA ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011650793.1
CDS	47..1453
Translation	MAFSSTLSRLNLIPKPSLPYALTTFKVLHFHKLLRTDWIGPGLSSNASTPRFSALKITSSAGFHTQISRPQDDDLIVLGIETSCDDTAAAIVKGNGEILSQVIASQAELLARYGGVAPKMAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ

Target ORF information:

RefSeq Version	XM_011652491.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial (LOC101216047), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011652491.1

ORF Insert Sequence:

ATGGCGTTTT CTTCCACACT TTCCCGCTTA AACCTTATCC CAAAACCTTC ACTTCCTTAC 
GCGCTTACTA CCTTCAAAGT TTTGCACTTC CACAAGTTAT TACGAACCGA TTGGATTGGA 
CCAGGTCTCT CATCTAATGC TTCAACTCCC CGATTTTCTG CTTTGAAAAT CACAAGCTCT 
GCTGGATTTC ACACTCAGAT TTCAAGGCCA CAAGACGATG ATTTGATTGT TCTCGGCATT 
GAAACGAGCT GCGATGATAC CGCCGCTGCC ATTGTGAAGG GCAATGGCGA AATCCTTAGC 
CAAGTTATAG CTTCTCAGGC AGAGCTGCTT GCTCGCTATG GAGGCGTTGC TCCTAAAATG 
GCAGAAGAAG CGCACGCACA AGTGATTGAC AAGGTGGTGC AGGAAACAAT GGAAAAGGCT 
AACTTAACAG AAAGGGATTT ATCTGCAGTT GCTGTGACCA TTGGCCCTGG TTTGAGCCTC 
TGCCTCCGTG TTGGAGTGCA GAAGGCACGA AAAATTGCTG GTAGCTCTAG TCTACCAATT 
GTTGGGGTGC ATCACATGGA AGCTCATGCT CTGGTAGCTA GATTAGTTGA ACGGGAACTG 
CAATTTCCTT TCATGGCACT GCTCATTTCT GGGGGACACA ATTTACTTGT TCTCGCACAA 
GATCTTGGCC AATACATACA ACTTGGAACA ACAATAGACG ATGCCATTGG GGAGGCGTAT 
GACAAGACTG CCAAATGGCT TGGTCTTGAT CTGAGGAAGA GTGGTGGGCC GGCTATAGAG 
GAGCTTGCCA GAGAGGGGGA TCCAAAGTCA ATAAAGTTTT CTACTCCAAT GAAGCAACAT 
AAAGATTGCA ACTTCTCCTA TGCGGGGCTG AAGACCCAAG TCAGGCTGGC TATTGAATCC 
CGAAACATTG ACACAAAAAT TCCTCTGTCG TCTGCAAGCA TCCAAGACAG AAGTGTAAGG 
GCTGATATAG CTGCCTCCTT TCAGCGTGTT GCAGTGCTAC ATCTAGAGGA CAAATGTGAA 
CGAGCAATTG AATGGGCGTT GAAGATTGAA CCATCCATAA GACATTTGGT TGTCTCAGGA 
GGTGTTGCAT CAAATAAGTA TGTTAGGGCT CAACTTATGG CGGTTGTCAG GAAGAAGGGC 
CTGCAGCTTG TCTGCCCTCC TCCCAGTCTC TGCACTGATA ATGGTGTAAT GGTGGCTTGG 
ACGGGCCTCG AGCATTTTCG CATAGGGAGA TTTGATCCTC CTCCACCAGC TGATGAACCT 
GAAGATACAA TGTATGACGT GCGACCGAGA TGGCCATTAG GAGAAGAATA CACCGAGGGA 
AGGAGTGCAG CTCGCTCACT AAGAACAGCC CGAATCCATC CATCACTTAC GTCTATCATC 
CAAGCATCTC TACAGCAACA ACAGTAG