LOC103486572 cDNA ORF clone, Cucumis melo(muskmelon)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103486572
Entrez Gene ID	103486572
Full Name	polyadenylation and cleavage factor homolog 4-like
Gene Type	protein-coding
Organism	Cucumis melo(muskmelon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008444576.2	XP_008442798.1	polyadenylation and cleavage factor homolog 4-like isoform X1
XM_008444577.2	XP_008442799.1	polyadenylation and cleavage factor homolog 4-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC103486572 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103486572 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu10740	XM_008444576.2 Latest version!	Cucumis melo polyadenylation and cleavage factor homolog 4-like (LOC103486572), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCu10741	XM_008444577.2 Latest version!	Cucumis melo polyadenylation and cleavage factor homolog 4-like (LOC103486572), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103486572 ( XM_008444576.2 ) cDNA ORF clone, Cucumis melo(muskmelon) -> XP_008442798.1 Cucumis melo polyadenylation and cleavage factor homolog 4-like (LOC103486572), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCu10740
Clone ID Related Accession (Same CDS sequence)	XM_008444576.2
Accession Version	XM_008444576.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cucumis melo(muskmelon)
Product	polyadenylation and cleavage factor homolog 4-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007546278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008444576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis melo Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATGG AGAGCTCGCG GAGACCTTTC GATCGAACTA GGGAACCGGG TTTGAAGAAG 
CCCCGACTCG CCGATGAGGC TGACCGCGGT GCCAATATTA ATGGCCGGCC GTTTCCGCAG 
AGACCAGTTG TGTCCGGGAA TAATATTGTG CAACAACCCA GATTTCGAGC AAGTGATAGG 
GATTCGGGAA GTAGTGATTC TGGTCGAGGG GGGTATCAGC CTCAGCCACC GCAGCATCAG 
GAGCTTGTGA GTCAGTACAG GACCGCCCTT GCCGAGCTGA CTTTCAATTC CAAACCAATC 
ATCACCAATT TGACCATAAT CGCGGGTGAA AATCTTCAGG CTGCCAAGGC GATCTCTACC 
ACCATTTACG CCAACATTCT CGAGGTTCCT AGCGAGCAGA AGCTACCGTC ACTTTATCTA 
CTGGACAGTA TTGTAAAGAA TATTGGAAGA GATTACATAA AATACTTTGC AGCAAGACTG 
CCGGAGGTAT TCTGCAAAGC TTATAGGCAG GTTGACCCTT CTGTACATCC AAGTATGAGA 
CATCTTTTTG GCACCTGGAA AGGAGTGTTT CCTCTTCAAA CTCTGCAGAT TATTGAGAAA 
GAACTTGGAT TCGTGCCTAC TGGTAGTTCT TCTTCTGTGG CCATAAACTC GAAGCCAGAT 
TTGCAGGCAC AACGTCCAAC TCCCCACAGT ATCCACGTAA ATCCAAAGTA TATAGAGCGA 
CAACGGCTTC AGCAGTCAGG CAGGGTGAAA GGAATGCCTA CTGATGCTAC TGGCGGTTCA 
ACAAATGTAA GTCAGGATGT TGCTCAAGCT AAAATTAGTA CTGGACGTCC ATGGGCGGAT 
GCTCCTATTA AGGTGCTTGA CATTCAGCGT CCCCTTAGAG ATGCACCAAA TGATATGGCA 
CAAGAGAAGA ACGTCACAGC TGGATACTCA GACTACGAAT ATGGTTCTGA TCTTTCAAGG 
ACATCAAGTG TCGGAAGAAG AGTCGTTGAT GAAGGGCGAG ACAAACCTTG GCCTTCAGCT 
GGAAGCAATT TGTCTGAGAA ACTATCTGGG CAAAGAAATG GGTTCAATAT CAAGCTTGGA 
TATGAAAATT ACTCTGCACC CAAGTCTACA AACACTGGTG CACGTTTACT GCCTGTGCAA 
AATTTTTCGA GCAGCAGCAA CAACAGAGTA TTGTCTACTA ACTGGAAGAA CTCTGAGGAA 
GAGGAGTTTA TGTGGGGTGA CATGAGCTCT ATGTTGACAG GTCATGGTGC ACCTGCTATC 
AACAGTAGCA CTGGAAAAGA CCAATGGACT CCTGAGGATT CGGATAATTC GGGTATTGAC 
AATAAGCATT TAAGCGTACG GGATACTGGG GCAAGTGTTG ATAGAGAAGC TTCCAGTGAT 
TCTCAGTCAT CTGAGCAGAG AGAACTGGGG GATTCTGGAC AGCAAAGGTC ATCAACGTGG 
CAACTGCAGG AGTCAATATC TCTGGATGGG CTGAGAGCTG GGGTTCCTAG AAAGAATTCA 
GGTCAGTCGG GAGGTTATGG TGCTACTCTC ACTGCACTGT CAGGTACAAA CTCTTCTGTG 
GATCAAATGG GAGGTCGACC ACAGATCACA CCATCTAATA TTGGGGCTTC AGGACATGGG 
TTTCTGAATA AAGGAGGTTC AGGGCCCCTT GGGAATGTAG GCCATCAAAG ATTTCCATCA 
CGAAGTGTTG CATTCCCATC TGGACAGCCA CCCTTGCACC AACGCTCACC TTCACAATTG 
TTAGTGGATC ATGTTCCTCA TCAAATACAT GACCAGAAAA CAACTTCATT TTCTAATCTT 
GATCCACGTA AAAGGCATAT GCAGGATGCT GCCCTTGGCC TGCATCCCAG CGTTCGACCA 
GATAATCATC AAAAGCCACA GACTCATGAC CTACGAGCTT TAGCTTCATC CATACCCGGG 
TCTCAACCAA GGCACCAGTT CTCTTTATCT GAGTCACTAA AACCTGATGT CACGCAGTCA 
GAACTTTCTA GTCAGCTTGC AGTATCAATT CCAGGCACTG ATTTTGGACC ATCATCATCA 
GCTGGGACAG TTCCAGATCG ATTACCTGCA GAAATTTTGG GAAACCCAAG CACAAGTAGT 
TTGTTGGCTG CTGTAATGAA GAGTGGACTT TTTTCCAACC ATTCAATAAC CAGTAACATG 
CAGCAGAATC TCAGCTTCCA AGATGTGGGA AATATGAAAC CCCGTTCAAG CATTAAACCC 
CCTCTACCAA ACCGGTCTTC TCCTGCCCAT ACATTCTCGG AGCCAAAGAT TCAGGGAGAA 
TCTTCAGTAG GTCCTCCATC AGTTGAAAGC CCATCAACTA TGGTTAAGTT GTCTCGGACA 
AAGGTAGAAG AACCATCATT GCCGTCTGAT CCACTTCCAC CTTCATCTCC TATGGATAGT 
GCATCTACTG AAACTTCACA TGTGGTAAAT GATGCTTCTA GTCCAATTTC TAACCTTTTG 
AGCTCATTGG TTGCAAAGGG GCTTATATCT GCTTCAAAAG GAGAATCAAC AAATAGCGTG 
ACATCCCAAA TGCCTTCACA GCCTGAAAAT TTGAAGTCAG GTGATGCTGT GACTAGTTCT 
GTACCAGTTC CTTCCATTGC TGTTTCCTCT TCCTGTCACT CATCTACGAA ACTCGAATCA 
CCTTTAAAAG CTGCTGCTAA GAGCTCTACT AGTCCACCTC CATCGGCCAC AACTGAGATA 
AACAACCTCA TAGGCTTTGA GTTTAGTTCA CATGTTATTC GCAAATTTCA TCCATCTGTG 
ATCAGTGGAC TCTTTGACGA TATTCCATAT CAATGTAAGA TCTGTGGTCT TAGACTGAAA 
TGTGAAGAAC AGTTGGATAC TCACTCACGG TGGCACACAT TAAGAACTGA GGCAAACAAT 
TCAAGTACAG CACCAAGAAG ATGGTATCCA TGTTCAGATG ATTGGATTTC TGGAAATGCT 
AGATTTCTAC TTGATGCTGA AACTTCTCTG GATGAATCTG ATTTGATGGA AGAAGATAAT 
GAGCCAATGG TTCCTGCTGA TGAAGATCAA TTTGCATGTG TTATATGTGG TGAACTTTTT 
GAAGATTTTT ATAGTCAAGA GTTGGGTAAT TGGATGTACA AAGGAGCAAC GTATATCACC 
ATCCCATCAG TGGGTAGCGA GGTTGGAGGT ACAAATGAAC AAGTTGCTAA AGGACCTATT 
GTACACACAA CTTGTTTAAC GGAAAGTTCA GTATATGACG TGGGACTGGC AACTGATATT 
AAGATGGAAA TGGATGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008442798.1
CDS	421..3681
Translation	MEMESSRRPFDRTREPGLKKPRLADEADRGANINGRPFPQRPVVSGNNIVQQPRFRASDRDSGSSDSGRGGYQPQPPQHQELVSQYRTALAELTFNSKPIITNLTIIAGENLQAAKAISTTIYANILEVPSEQKLPSLYLLDSIVKNIGRDYIKYFAARLPEVFCKAYRQVDPSVHPSMRHLFGTWKGVFPLQTLQIIEKELGFVPTGSSSSVAINSKPDLQAQRPTPHSIHVNPKYIERQRLQQSGRVKGMPTDATGGSTNVSQDVAQAKISTGRPWADAPIKVLDIQRPLRDAPNDMAQEKNVTAGYSDYEYGSDLSRTSSVGRRVVDEGRDKPWPSAGSNLSEKLSGQRNGFNIKLGYENYSAPKSTNTGARLLPVQNFSSSSNNRVLSTNWKNSEEEEFMWGDMSSMLTGHGAPAINSSTGKDQWTPEDSDNSGIDNKHLSVRDTGASVDREASSDSQSSEQRELGDSGQQRSSTWQLQESISLDGLRAGVPRKNSGQSGGYGATLTALSGTNSSVDQMGGRPQITPSNIGASGHGFLNKGGSGPLGNVGHQRFPSRSVAFPSGQPPLHQRSPSQLLVDHVPHQIHDQKTTSFSNLDPRKRHMQDAALGLHPSVRPDNHQKPQTHDLRALASSIPGSQPRHQFSLSESLKPDVTQSELSSQLAVSIPGTDFGPSSSAGTVPDRLPAEILGNPSTSSLLAAVMKSGLFSNHSITSNMQQNLSFQDVGNMKPRSSIKPPLPNRSSPAHTFSEPKIQGESSVGPPSVESPSTMVKLSRTKVEEPSLPSDPLPPSSPMDSASTETSHVVNDASSPISNLLSSLVAKGLISASKGESTNSVTSQMPSQPENLKSGDAVTSSVPVPSIAVSSSCHSSTKLESPLKAAAKSSTSPPPSATTEINNLIGFEFSSHVIRKFHPSVISGLFDDIPYQCKICGLRLKCEEQLDTHSRWHTLRTEANNSSTAPRRWYPCSDDWISGNARFLLDAETSLDESDLMEEDNEPMVPADEDQFACVICGELFEDFYSQELGNWMYKGATYITIPSVGSEVGGTNEQVAKGPIVHTTCLTESSVYDVGLATDIKMEMDV

Target ORF information:

RefSeq Version	XM_008444576.2
Organism	Cucumis melo(muskmelon)
Definition	Cucumis melo polyadenylation and cleavage factor homolog 4-like (LOC103486572), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008444576.2

ORF Insert Sequence:

ATGGAAATGG AGAGCTCGCG GAGACCTTTC GATCGAACTA GGGAACCGGG TTTGAAGAAG 
CCCCGACTCG CCGATGAGGC TGACCGCGGT GCCAATATTA ATGGCCGGCC GTTTCCGCAG 
AGACCAGTTG TGTCCGGGAA TAATATTGTG CAACAACCCA GATTTCGAGC AAGTGATAGG 
GATTCGGGAA GTAGTGATTC TGGTCGAGGG GGGTATCAGC CTCAGCCACC GCAGCATCAG 
GAGCTTGTGA GTCAGTACAG GACCGCCCTT GCCGAGCTGA CTTTCAATTC CAAACCAATC 
ATCACCAATT TGACCATAAT CGCGGGTGAA AATCTTCAGG CTGCCAAGGC GATCTCTACC 
ACCATTTACG CCAACATTCT CGAGGTTCCT AGCGAGCAGA AGCTACCGTC ACTTTATCTA 
CTGGACAGTA TTGTAAAGAA TATTGGAAGA GATTACATAA AATACTTTGC AGCAAGACTG 
CCGGAGGTAT TCTGCAAAGC TTATAGGCAG GTTGACCCTT CTGTACATCC AAGTATGAGA 
CATCTTTTTG GCACCTGGAA AGGAGTGTTT CCTCTTCAAA CTCTGCAGAT TATTGAGAAA 
GAACTTGGAT TCGTGCCTAC TGGTAGTTCT TCTTCTGTGG CCATAAACTC GAAGCCAGAT 
TTGCAGGCAC AACGTCCAAC TCCCCACAGT ATCCACGTAA ATCCAAAGTA TATAGAGCGA 
CAACGGCTTC AGCAGTCAGG CAGGGTGAAA GGAATGCCTA CTGATGCTAC TGGCGGTTCA 
ACAAATGTAA GTCAGGATGT TGCTCAAGCT AAAATTAGTA CTGGACGTCC ATGGGCGGAT 
GCTCCTATTA AGGTGCTTGA CATTCAGCGT CCCCTTAGAG ATGCACCAAA TGATATGGCA 
CAAGAGAAGA ACGTCACAGC TGGATACTCA GACTACGAAT ATGGTTCTGA TCTTTCAAGG 
ACATCAAGTG TCGGAAGAAG AGTCGTTGAT GAAGGGCGAG ACAAACCTTG GCCTTCAGCT 
GGAAGCAATT TGTCTGAGAA ACTATCTGGG CAAAGAAATG GGTTCAATAT CAAGCTTGGA 
TATGAAAATT ACTCTGCACC CAAGTCTACA AACACTGGTG CACGTTTACT GCCTGTGCAA 
AATTTTTCGA GCAGCAGCAA CAACAGAGTA TTGTCTACTA ACTGGAAGAA CTCTGAGGAA 
GAGGAGTTTA TGTGGGGTGA CATGAGCTCT ATGTTGACAG GTCATGGTGC ACCTGCTATC 
AACAGTAGCA CTGGAAAAGA CCAATGGACT CCTGAGGATT CGGATAATTC GGGTATTGAC 
AATAAGCATT TAAGCGTACG GGATACTGGG GCAAGTGTTG ATAGAGAAGC TTCCAGTGAT 
TCTCAGTCAT CTGAGCAGAG AGAACTGGGG GATTCTGGAC AGCAAAGGTC ATCAACGTGG 
CAACTGCAGG AGTCAATATC TCTGGATGGG CTGAGAGCTG GGGTTCCTAG AAAGAATTCA 
GGTCAGTCGG GAGGTTATGG TGCTACTCTC ACTGCACTGT CAGGTACAAA CTCTTCTGTG 
GATCAAATGG GAGGTCGACC ACAGATCACA CCATCTAATA TTGGGGCTTC AGGACATGGG 
TTTCTGAATA AAGGAGGTTC AGGGCCCCTT GGGAATGTAG GCCATCAAAG ATTTCCATCA 
CGAAGTGTTG CATTCCCATC TGGACAGCCA CCCTTGCACC AACGCTCACC TTCACAATTG 
TTAGTGGATC ATGTTCCTCA TCAAATACAT GACCAGAAAA CAACTTCATT TTCTAATCTT 
GATCCACGTA AAAGGCATAT GCAGGATGCT GCCCTTGGCC TGCATCCCAG CGTTCGACCA 
GATAATCATC AAAAGCCACA GACTCATGAC CTACGAGCTT TAGCTTCATC CATACCCGGG 
TCTCAACCAA GGCACCAGTT CTCTTTATCT GAGTCACTAA AACCTGATGT CACGCAGTCA 
GAACTTTCTA GTCAGCTTGC AGTATCAATT CCAGGCACTG ATTTTGGACC ATCATCATCA 
GCTGGGACAG TTCCAGATCG ATTACCTGCA GAAATTTTGG GAAACCCAAG CACAAGTAGT 
TTGTTGGCTG CTGTAATGAA GAGTGGACTT TTTTCCAACC ATTCAATAAC CAGTAACATG 
CAGCAGAATC TCAGCTTCCA AGATGTGGGA AATATGAAAC CCCGTTCAAG CATTAAACCC 
CCTCTACCAA ACCGGTCTTC TCCTGCCCAT ACATTCTCGG AGCCAAAGAT TCAGGGAGAA 
TCTTCAGTAG GTCCTCCATC AGTTGAAAGC CCATCAACTA TGGTTAAGTT GTCTCGGACA 
AAGGTAGAAG AACCATCATT GCCGTCTGAT CCACTTCCAC CTTCATCTCC TATGGATAGT 
GCATCTACTG AAACTTCACA TGTGGTAAAT GATGCTTCTA GTCCAATTTC TAACCTTTTG 
AGCTCATTGG TTGCAAAGGG GCTTATATCT GCTTCAAAAG GAGAATCAAC AAATAGCGTG 
ACATCCCAAA TGCCTTCACA GCCTGAAAAT TTGAAGTCAG GTGATGCTGT GACTAGTTCT 
GTACCAGTTC CTTCCATTGC TGTTTCCTCT TCCTGTCACT CATCTACGAA ACTCGAATCA 
CCTTTAAAAG CTGCTGCTAA GAGCTCTACT AGTCCACCTC CATCGGCCAC AACTGAGATA 
AACAACCTCA TAGGCTTTGA GTTTAGTTCA CATGTTATTC GCAAATTTCA TCCATCTGTG 
ATCAGTGGAC TCTTTGACGA TATTCCATAT CAATGTAAGA TCTGTGGTCT TAGACTGAAA 
TGTGAAGAAC AGTTGGATAC TCACTCACGG TGGCACACAT TAAGAACTGA GGCAAACAAT 
TCAAGTACAG CACCAAGAAG ATGGTATCCA TGTTCAGATG ATTGGATTTC TGGAAATGCT 
AGATTTCTAC TTGATGCTGA AACTTCTCTG GATGAATCTG ATTTGATGGA AGAAGATAAT 
GAGCCAATGG TTCCTGCTGA TGAAGATCAA TTTGCATGTG TTATATGTGG TGAACTTTTT 
GAAGATTTTT ATAGTCAAGA GTTGGGTAAT TGGATGTACA AAGGAGCAAC GTATATCACC 
ATCCCATCAG TGGGTAGCGA GGTTGGAGGT ACAAATGAAC AAGTTGCTAA AGGACCTATT 
GTACACACAA CTTGTTTAAC GGAAAGTTCA GTATATGACG TGGGACTGGC AACTGATATT 
AAGATGGAAA TGGATGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103486572 ( XM_008444577.2 ) cDNA ORF clone, Cucumis melo(muskmelon) -> XP_008442799.1 Cucumis melo polyadenylation and cleavage factor homolog 4-like (LOC103486572), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCu10741
Clone ID Related Accession (Same CDS sequence)	XM_008444577.2
Accession Version	XM_008444577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cucumis melo(muskmelon)
Product	polyadenylation and cleavage factor homolog 4-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007546278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008444577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis melo Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATGG AGAGCTCGCG GAGACCTTTC GATCGAACTA GGGAACCGGG TTTGAAGAAG 
CCCCGACTCG CCGATGAGGC TGACCGCGGT GCCAATATTA ATGGCCGGCC GTTTCCGCAG 
AGACCAGTTG TGTCCGGGAA TAATATTGTG CAACAACCCA GATTTCGAGC AAGTGATAGG 
GATTCGGGAA GTAGTGATTC TGGTCGAGGG GGGTATCAGC CTCAGCCACC GCAGCATCAG 
GAGCTTGTGA GTCAGTACAG GACCGCCCTT GCCGAGCTGA CTTTCAATTC CAAACCAATC 
ATCACCAATT TGACCATAAT CGCGGGTGAA AATCTTCAGG CTGCCAAGGC GATCTCTACC 
ACCATTTACG CCAACATTCT CGAGGTTCCT AGCGAGCAGA AGCTACCGTC ACTTTATCTA 
CTGGACAGTA TTGTAAAGAA TATTGGAAGA GATTACATAA AATACTTTGC AGCAAGACTG 
CCGGAGGTAT TCTGCAAAGC TTATAGGCAG GTTGACCCTT CTGTACATCC AAGTATGAGA 
CATCTTTTTG GCACCTGGAA AGGAGTGTTT CCTCTTCAAA CTCTGCAGAT TATTGAGAAA 
GAACTTGGAT TCGTGCCTAC TGGTAGTTCT TCTTCTGTGG CCATAAACTC GAAGCCAGAT 
TTGCAGGCAC AACGTCCAAC TCCCCACAGT ATCCACGTAA ATCCAAAGTA TATAGAGCGA 
CAACGGCTTC AGCAGTCAGG CAGGGTGAAA GGAATGCCTA CTGATGCTAC TGGCGGTTCA 
ACAAATGTAA GTCAGGATGT TGCTCAAGCT AAAATTAGTA CTGGACGTCC ATGGGCGGAT 
GCTCCTATTA AGGACATTCA GCGTCCCCTT AGAGATGCAC CAAATGATAT GGCACAAGAG 
AAGAACGTCA CAGCTGGATA CTCAGACTAC GAATATGGTT CTGATCTTTC AAGGACATCA 
AGTGTCGGAA GAAGAGTCGT TGATGAAGGG CGAGACAAAC CTTGGCCTTC AGCTGGAAGC 
AATTTGTCTG AGAAACTATC TGGGCAAAGA AATGGGTTCA ATATCAAGCT TGGATATGAA 
AATTACTCTG CACCCAAGTC TACAAACACT GGTGCACGTT TACTGCCTGT GCAAAATTTT 
TCGAGCAGCA GCAACAACAG AGTATTGTCT ACTAACTGGA AGAACTCTGA GGAAGAGGAG 
TTTATGTGGG GTGACATGAG CTCTATGTTG ACAGGTCATG GTGCACCTGC TATCAACAGT 
AGCACTGGAA AAGACCAATG GACTCCTGAG GATTCGGATA ATTCGGGTAT TGACAATAAG 
CATTTAAGCG TACGGGATAC TGGGGCAAGT GTTGATAGAG AAGCTTCCAG TGATTCTCAG 
TCATCTGAGC AGAGAGAACT GGGGGATTCT GGACAGCAAA GGTCATCAAC GTGGCAACTG 
CAGGAGTCAA TATCTCTGGA TGGGCTGAGA GCTGGGGTTC CTAGAAAGAA TTCAGGTCAG 
TCGGGAGGTT ATGGTGCTAC TCTCACTGCA CTGTCAGGTA CAAACTCTTC TGTGGATCAA 
ATGGGAGGTC GACCACAGAT CACACCATCT AATATTGGGG CTTCAGGACA TGGGTTTCTG 
AATAAAGGAG GTTCAGGGCC CCTTGGGAAT GTAGGCCATC AAAGATTTCC ATCACGAAGT 
GTTGCATTCC CATCTGGACA GCCACCCTTG CACCAACGCT CACCTTCACA ATTGTTAGTG 
GATCATGTTC CTCATCAAAT ACATGACCAG AAAACAACTT CATTTTCTAA TCTTGATCCA 
CGTAAAAGGC ATATGCAGGA TGCTGCCCTT GGCCTGCATC CCAGCGTTCG ACCAGATAAT 
CATCAAAAGC CACAGACTCA TGACCTACGA GCTTTAGCTT CATCCATACC CGGGTCTCAA 
CCAAGGCACC AGTTCTCTTT ATCTGAGTCA CTAAAACCTG ATGTCACGCA GTCAGAACTT 
TCTAGTCAGC TTGCAGTATC AATTCCAGGC ACTGATTTTG GACCATCATC ATCAGCTGGG 
ACAGTTCCAG ATCGATTACC TGCAGAAATT TTGGGAAACC CAAGCACAAG TAGTTTGTTG 
GCTGCTGTAA TGAAGAGTGG ACTTTTTTCC AACCATTCAA TAACCAGTAA CATGCAGCAG 
AATCTCAGCT TCCAAGATGT GGGAAATATG AAACCCCGTT CAAGCATTAA ACCCCCTCTA 
CCAAACCGGT CTTCTCCTGC CCATACATTC TCGGAGCCAA AGATTCAGGG AGAATCTTCA 
GTAGGTCCTC CATCAGTTGA AAGCCCATCA ACTATGGTTA AGTTGTCTCG GACAAAGGTA 
GAAGAACCAT CATTGCCGTC TGATCCACTT CCACCTTCAT CTCCTATGGA TAGTGCATCT 
ACTGAAACTT CACATGTGGT AAATGATGCT TCTAGTCCAA TTTCTAACCT TTTGAGCTCA 
TTGGTTGCAA AGGGGCTTAT ATCTGCTTCA AAAGGAGAAT CAACAAATAG CGTGACATCC 
CAAATGCCTT CACAGCCTGA AAATTTGAAG TCAGGTGATG CTGTGACTAG TTCTGTACCA 
GTTCCTTCCA TTGCTGTTTC CTCTTCCTGT CACTCATCTA CGAAACTCGA ATCACCTTTA 
AAAGCTGCTG CTAAGAGCTC TACTAGTCCA CCTCCATCGG CCACAACTGA GATAAACAAC 
CTCATAGGCT TTGAGTTTAG TTCACATGTT ATTCGCAAAT TTCATCCATC TGTGATCAGT 
GGACTCTTTG ACGATATTCC ATATCAATGT AAGATCTGTG GTCTTAGACT GAAATGTGAA 
GAACAGTTGG ATACTCACTC ACGGTGGCAC ACATTAAGAA CTGAGGCAAA CAATTCAAGT 
ACAGCACCAA GAAGATGGTA TCCATGTTCA GATGATTGGA TTTCTGGAAA TGCTAGATTT 
CTACTTGATG CTGAAACTTC TCTGGATGAA TCTGATTTGA TGGAAGAAGA TAATGAGCCA 
ATGGTTCCTG CTGATGAAGA TCAATTTGCA TGTGTTATAT GTGGTGAACT TTTTGAAGAT 
TTTTATAGTC AAGAGTTGGG TAATTGGATG TACAAAGGAG CAACGTATAT CACCATCCCA 
TCAGTGGGTA GCGAGGTTGG AGGTACAAAT GAACAAGTTG CTAAAGGACC TATTGTACAC 
ACAACTTGTT TAACGGAAAG TTCAGTATAT GACGTGGGAC TGGCAACTGA TATTAAGATG 
GAAATGGATG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008442799.1
CDS	421..3675
Translation	MEMESSRRPFDRTREPGLKKPRLADEADRGANINGRPFPQRPVVSGNNIVQQPRFRASDRDSGSSDSGRGGYQPQPPQHQELVSQYRTALAELTFNSKPIITNLTIIAGENLQAAKAISTTIYANILEVPSEQKLPSLYLLDSIVKNIGRDYIKYFAARLPEVFCKAYRQVDPSVHPSMRHLFGTWKGVFPLQTLQIIEKELGFVPTGSSSSVAINSKPDLQAQRPTPHSIHVNPKYIERQRLQQSGRVKGMPTDATGGSTNVSQDVAQAKISTGRPWADAPIKDIQRPLRDAPNDMAQEKNVTAGYSDYEYGSDLSRTSSVGRRVVDEGRDKPWPSAGSNLSEKLSGQRNGFNIKLGYENYSAPKSTNTGARLLPVQNFSSSSNNRVLSTNWKNSEEEEFMWGDMSSMLTGHGAPAINSSTGKDQWTPEDSDNSGIDNKHLSVRDTGASVDREASSDSQSSEQRELGDSGQQRSSTWQLQESISLDGLRAGVPRKNSGQSGGYGATLTALSGTNSSVDQMGGRPQITPSNIGASGHGFLNKGGSGPLGNVGHQRFPSRSVAFPSGQPPLHQRSPSQLLVDHVPHQIHDQKTTSFSNLDPRKRHMQDAALGLHPSVRPDNHQKPQTHDLRALASSIPGSQPRHQFSLSESLKPDVTQSELSSQLAVSIPGTDFGPSSSAGTVPDRLPAEILGNPSTSSLLAAVMKSGLFSNHSITSNMQQNLSFQDVGNMKPRSSIKPPLPNRSSPAHTFSEPKIQGESSVGPPSVESPSTMVKLSRTKVEEPSLPSDPLPPSSPMDSASTETSHVVNDASSPISNLLSSLVAKGLISASKGESTNSVTSQMPSQPENLKSGDAVTSSVPVPSIAVSSSCHSSTKLESPLKAAAKSSTSPPPSATTEINNLIGFEFSSHVIRKFHPSVISGLFDDIPYQCKICGLRLKCEEQLDTHSRWHTLRTEANNSSTAPRRWYPCSDDWISGNARFLLDAETSLDESDLMEEDNEPMVPADEDQFACVICGELFEDFYSQELGNWMYKGATYITIPSVGSEVGGTNEQVAKGPIVHTTCLTESSVYDVGLATDIKMEMDV

Target ORF information:

RefSeq Version	XM_008444577.2
Organism	Cucumis melo(muskmelon)
Definition	Cucumis melo polyadenylation and cleavage factor homolog 4-like (LOC103486572), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008444577.2

ORF Insert Sequence:

ATGGAAATGG AGAGCTCGCG GAGACCTTTC GATCGAACTA GGGAACCGGG TTTGAAGAAG 
CCCCGACTCG CCGATGAGGC TGACCGCGGT GCCAATATTA ATGGCCGGCC GTTTCCGCAG 
AGACCAGTTG TGTCCGGGAA TAATATTGTG CAACAACCCA GATTTCGAGC AAGTGATAGG 
GATTCGGGAA GTAGTGATTC TGGTCGAGGG GGGTATCAGC CTCAGCCACC GCAGCATCAG 
GAGCTTGTGA GTCAGTACAG GACCGCCCTT GCCGAGCTGA CTTTCAATTC CAAACCAATC 
ATCACCAATT TGACCATAAT CGCGGGTGAA AATCTTCAGG CTGCCAAGGC GATCTCTACC 
ACCATTTACG CCAACATTCT CGAGGTTCCT AGCGAGCAGA AGCTACCGTC ACTTTATCTA 
CTGGACAGTA TTGTAAAGAA TATTGGAAGA GATTACATAA AATACTTTGC AGCAAGACTG 
CCGGAGGTAT TCTGCAAAGC TTATAGGCAG GTTGACCCTT CTGTACATCC AAGTATGAGA 
CATCTTTTTG GCACCTGGAA AGGAGTGTTT CCTCTTCAAA CTCTGCAGAT TATTGAGAAA 
GAACTTGGAT TCGTGCCTAC TGGTAGTTCT TCTTCTGTGG CCATAAACTC GAAGCCAGAT 
TTGCAGGCAC AACGTCCAAC TCCCCACAGT ATCCACGTAA ATCCAAAGTA TATAGAGCGA 
CAACGGCTTC AGCAGTCAGG CAGGGTGAAA GGAATGCCTA CTGATGCTAC TGGCGGTTCA 
ACAAATGTAA GTCAGGATGT TGCTCAAGCT AAAATTAGTA CTGGACGTCC ATGGGCGGAT 
GCTCCTATTA AGGACATTCA GCGTCCCCTT AGAGATGCAC CAAATGATAT GGCACAAGAG 
AAGAACGTCA CAGCTGGATA CTCAGACTAC GAATATGGTT CTGATCTTTC AAGGACATCA 
AGTGTCGGAA GAAGAGTCGT TGATGAAGGG CGAGACAAAC CTTGGCCTTC AGCTGGAAGC 
AATTTGTCTG AGAAACTATC TGGGCAAAGA AATGGGTTCA ATATCAAGCT TGGATATGAA 
AATTACTCTG CACCCAAGTC TACAAACACT GGTGCACGTT TACTGCCTGT GCAAAATTTT 
TCGAGCAGCA GCAACAACAG AGTATTGTCT ACTAACTGGA AGAACTCTGA GGAAGAGGAG 
TTTATGTGGG GTGACATGAG CTCTATGTTG ACAGGTCATG GTGCACCTGC TATCAACAGT 
AGCACTGGAA AAGACCAATG GACTCCTGAG GATTCGGATA ATTCGGGTAT TGACAATAAG 
CATTTAAGCG TACGGGATAC TGGGGCAAGT GTTGATAGAG AAGCTTCCAG TGATTCTCAG 
TCATCTGAGC AGAGAGAACT GGGGGATTCT GGACAGCAAA GGTCATCAAC GTGGCAACTG 
CAGGAGTCAA TATCTCTGGA TGGGCTGAGA GCTGGGGTTC CTAGAAAGAA TTCAGGTCAG 
TCGGGAGGTT ATGGTGCTAC TCTCACTGCA CTGTCAGGTA CAAACTCTTC TGTGGATCAA 
ATGGGAGGTC GACCACAGAT CACACCATCT AATATTGGGG CTTCAGGACA TGGGTTTCTG 
AATAAAGGAG GTTCAGGGCC CCTTGGGAAT GTAGGCCATC AAAGATTTCC ATCACGAAGT 
GTTGCATTCC CATCTGGACA GCCACCCTTG CACCAACGCT CACCTTCACA ATTGTTAGTG 
GATCATGTTC CTCATCAAAT ACATGACCAG AAAACAACTT CATTTTCTAA TCTTGATCCA 
CGTAAAAGGC ATATGCAGGA TGCTGCCCTT GGCCTGCATC CCAGCGTTCG ACCAGATAAT 
CATCAAAAGC CACAGACTCA TGACCTACGA GCTTTAGCTT CATCCATACC CGGGTCTCAA 
CCAAGGCACC AGTTCTCTTT ATCTGAGTCA CTAAAACCTG ATGTCACGCA GTCAGAACTT 
TCTAGTCAGC TTGCAGTATC AATTCCAGGC ACTGATTTTG GACCATCATC ATCAGCTGGG 
ACAGTTCCAG ATCGATTACC TGCAGAAATT TTGGGAAACC CAAGCACAAG TAGTTTGTTG 
GCTGCTGTAA TGAAGAGTGG ACTTTTTTCC AACCATTCAA TAACCAGTAA CATGCAGCAG 
AATCTCAGCT TCCAAGATGT GGGAAATATG AAACCCCGTT CAAGCATTAA ACCCCCTCTA 
CCAAACCGGT CTTCTCCTGC CCATACATTC TCGGAGCCAA AGATTCAGGG AGAATCTTCA 
GTAGGTCCTC CATCAGTTGA AAGCCCATCA ACTATGGTTA AGTTGTCTCG GACAAAGGTA 
GAAGAACCAT CATTGCCGTC TGATCCACTT CCACCTTCAT CTCCTATGGA TAGTGCATCT 
ACTGAAACTT CACATGTGGT AAATGATGCT TCTAGTCCAA TTTCTAACCT TTTGAGCTCA 
TTGGTTGCAA AGGGGCTTAT ATCTGCTTCA AAAGGAGAAT CAACAAATAG CGTGACATCC 
CAAATGCCTT CACAGCCTGA AAATTTGAAG TCAGGTGATG CTGTGACTAG TTCTGTACCA 
GTTCCTTCCA TTGCTGTTTC CTCTTCCTGT CACTCATCTA CGAAACTCGA ATCACCTTTA 
AAAGCTGCTG CTAAGAGCTC TACTAGTCCA CCTCCATCGG CCACAACTGA GATAAACAAC 
CTCATAGGCT TTGAGTTTAG TTCACATGTT ATTCGCAAAT TTCATCCATC TGTGATCAGT 
GGACTCTTTG ACGATATTCC ATATCAATGT AAGATCTGTG GTCTTAGACT GAAATGTGAA 
GAACAGTTGG ATACTCACTC ACGGTGGCAC ACATTAAGAA CTGAGGCAAA CAATTCAAGT 
ACAGCACCAA GAAGATGGTA TCCATGTTCA GATGATTGGA TTTCTGGAAA TGCTAGATTT 
CTACTTGATG CTGAAACTTC TCTGGATGAA TCTGATTTGA TGGAAGAAGA TAATGAGCCA 
ATGGTTCCTG CTGATGAAGA TCAATTTGCA TGTGTTATAT GTGGTGAACT TTTTGAAGAT 
TTTTATAGTC AAGAGTTGGG TAATTGGATG TACAAAGGAG CAACGTATAT CACCATCCCA 
TCAGTGGGTA GCGAGGTTGG AGGTACAAAT GAACAAGTTG CTAAAGGACC TATTGTACAC 
ACAACTTGTT TAACGGAAAG TTCAGTATAT GACGTGGGAC TGGCAACTGA TATTAAGATG 
GAAATGGATG TATGA