NGLY1 cDNA ORF clone, Cuculus canorus(common cuckoo)

Gene
Clones
gRNAs

Summary

Gene Symbol	NGLY1
Entrez Gene ID	104067575
Full Name	N-glycanase 1
Gene Type	protein-coding
Organism	Cuculus canorus(common cuckoo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009570345.1	XP_009568640.1	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase

Pathways from BioSystems
Gene Ontology

The following NGLY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGLY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu38436	XM_009570345.1 Latest version!	Cuculus canorus N-glycanase 1 (NGLY1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NGLY1 ( XM_009570345.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009568640.1 Cuculus canorus N-glycanase 1 (NGLY1), mRNA.

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CloneID	OCu38436
Clone ID Related Accession (Same CDS sequence)	XM_009570345.1
Accession Version	XM_009570345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009243687.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 11% of CDS bases ##RefSeq-Attributes-END##

ATGGCTTGCT GGTCCATGGG TGCCAAAGGT GCCTTTGGGC ACCGCGGGGG CAGAGGGCAA 
GCAACGGCAG CCAAACAATT CCAGAGCTTG GGCGGGCGCG TTGTTAGGAA AACAGAGCCC 
ACTGGTCTAC TTGGCAATTC CTGTTGGGTG TCTCTGTACG GTGTACACAT GGCAATACGT 
ATGATTGACC TTGATGTTGT CTGCAAGCAA CTCAAGATGT ACAAAATGCC CCTCCCTGTC 
TATATCTGGA ATCCTTATGA AGAGAAATAT AGATTAATTC GCATTGGAAA TCCAGCTTTT 
TCTACAAGGC TGTTGCCTGT CAAAGGAGCA GTTCAATGTT TATTTGAGAT GGGGTTTCAA 
GAGGGAGAAA CTCATATGAT TTTCCCTAAG GAAGCTTCCA TTGAGCAGCT ACGTAAAATC 
AGAGACTTAA TTGCTGGAGA GAGGAGTAGC AGGCTGAATG AATCAAATCC AACCCACAGA 
CCAGGATCCT CTGAAATCGT GGCCAACACT CGGGCAGTTG CACATCAGCC TTCGAGACCT 
GCTGACTCTG CTCTTGCCCC TGCCCCTCAG CAACCAGAAA CATCACTTGT GCAGTCTCTT 
AAAACGGCTG CAAAAATCTT GAGTACACTT CAAACAAAAT TTAAGCATCA TGTATTGATA 
TACGAGAAAC CTTCTCTTCA GCAGAAAGCA CTGGCTTCAA TTCCCCTCCA GCAACTGAAA 
GGGAAAGCTC AGAAAAAGCT AACACAAGCT ACAAGACTGG ACAAAGGTGC ACATGTGAAT 
GAAGAGGACT TCTTGTTGTT GGAACTCTTG GACTGGTTTA AGAAGGATTT TTTTCATTGG 
GTAAACGCAC TTCCTTGCAG CAGGTGTGGT GGGGAGACAG TGCCTCAAAG TGAATATCTG 
TTGCCTACTG AAGGCGATCT AAGATGGAAT GCTAGTCGAG TGGAAAATCA TTACTGCAAC 
CAGTGTCAGA TTTGTAATAG ATTTCCAAGA TATAATGACC CTGAAAAACT TCTGGAAACC 
AGACGTGGAC GCTGTGGCGA GTGGGCTAAT TGTTTTACAC TGTGTTGCAG AGCTGTGGGG 
TTTGAAGCTA GATACGTTTG GGACTGTACA GATCATCTGT GGACTGAAGT ATATTCTTCA 
TCTCAGAAAA GATGGCTTCA CTGTGATCCT TGTGAAAATG TCTGTGATAA GCCTCTTATC 
TACGAAACTG GGTGGGGAAA GAAGCTCTCC TACATCTTTG CATTCTCCAA AGATGAGGTT 
GTTGATGTCA CCTGGAGATA CAGCTGTAAG CATGAAGAAG TGCTCTCTAG AAGGACTGCA 
CTCAGTGAAG CTACACTACG TGGAACAATT AATGCACTAA ATAGAATGAG GCAACGATCT 
TTGTCAGAAA ATAGAAGAAG AGAGCTCTTG GAAAGAACAA TTGTGGAGCT TGTTGAGTTT 
ATTTCTCCTA AAACACCTAA GCCTGGAGAA TATGGTGGAA GGACATCAGG TTCAATGGCT 
TGGCGAGTAG CCAGAGGTGA AATTGGTTCA GAGAAAAGAA GAGAAGTAAT TTTTGTTCCC 
TCTGGAAAAG AGAAGACATC TAAACTCTTC CACCTCATTT ACAATGTAGT AGAAGATAGT 
TATACACGGA TTTCCAATAA TAATGAAAAA ATAACTGGCT GGGAAGCAGG TGTTTGGAAA 
GCAGAGTCTG TCTGGAGAAA GGTTGAAACA GATTGGAAAA TGGTATATTT AGCCCGAAAA 
GAGGGGTCAT CCTCTGCTTC TGTCAGCTGG AAGTTTGAAT GCAAATCAGT TGGTCTGAAA 
ATAGAAAACA TTTCAGTTAG GACAAGCAGT CAGACATTTC ATAGTGGAAG AATAAAGTGG 
AGACTTTACT CTCCAACAGC AGAAATTGCT TTGATGGGAG ACAAAAATCT TTGCTCCTGT 
TCAGATTTTT CTGGTGCAAC GGAAGTTGTT TTGGAAGCAA TTCTGAATGG AGGGGATGGT 
GAAAGTGCAT GGCAACACAC ACAGCTGTTT AGAGACAGCT TAACAGGATG TGGAGAAAAT 
TGCTTGGAGA TAATTATAAA ACTCACTGAC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009568640.1
CDS	1..2076
Translation	MACWSMGAKGAFGHRGGRGQATAAKQFQSLGGRVVRKTEPTGLLGNSCWVSLYGVHMAIRMIDLDVVCKQLKMYKMPLPVYIWNPYEEKYRLIRIGNPAFSTRLLPVKGAVQCLFEMGFQEGETHMIFPKEASIEQLRKIRDLIAGERSSRLNESNPTHRPGSSEIVANTRAVAHQPSRPADSALAPAPQQPETSLVQSLKTAAKILSTLQTKFKHHVLIYEKPSLQQKALASIPLQQLKGKAQKKLTQATRLDKGAHVNEEDFLLLELLDWFKKDFFHWVNALPCSRCGGETVPQSEYLLPTEGDLRWNASRVENHYCNQCQICNRFPRYNDPEKLLETRRGRCGEWANCFTLCCRAVGFEARYVWDCTDHLWTEVYSSSQKRWLHCDPCENVCDKPLIYETGWGKKLSYIFAFSKDEVVDVTWRYSCKHEEVLSRRTALSEATLRGTINALNRMRQRSLSENRRRELLERTIVELVEFISPKTPKPGEYGGRTSGSMAWRVARGEIGSEKRREVIFVPSGKEKTSKLFHLIYNVVEDSYTRISNNNEKITGWEAGVWKAESVWRKVETDWKMVYLARKEGSSSASVSWKFECKSVGLKIENISVRTSSQTFHSGRIKWRLYSPTAEIALMGDKNLCSCSDFSGATEVVLEAILNGGDGESAWQHTQLFRDSLTGCGENCLEIIIKLTDL

Target ORF information:

RefSeq Version	XM_009570345.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus N-glycanase 1 (NGLY1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009570345.1

ORF Insert Sequence:

ATGGCTTGCT GGTCCATGGG TGCCAAAGGT GCCTTTGGGC ACCGCGGGGG CAGAGGGCAA 
GCAACGGCAG CCAAACAATT CCAGAGCTTG GGCGGGCGCG TTGTTAGGAA AACAGAGCCC 
ACTGGTCTAC TTGGCAATTC CTGTTGGGTG TCTCTGTACG GTGTACACAT GGCAATACGT 
ATGATTGACC TTGATGTTGT CTGCAAGCAA CTCAAGATGT ACAAAATGCC CCTCCCTGTC 
TATATCTGGA ATCCTTATGA AGAGAAATAT AGATTAATTC GCATTGGAAA TCCAGCTTTT 
TCTACAAGGC TGTTGCCTGT CAAAGGAGCA GTTCAATGTT TATTTGAGAT GGGGTTTCAA 
GAGGGAGAAA CTCATATGAT TTTCCCTAAG GAAGCTTCCA TTGAGCAGCT ACGTAAAATC 
AGAGACTTAA TTGCTGGAGA GAGGAGTAGC AGGCTGAATG AATCAAATCC AACCCACAGA 
CCAGGATCCT CTGAAATCGT GGCCAACACT CGGGCAGTTG CACATCAGCC TTCGAGACCT 
GCTGACTCTG CTCTTGCCCC TGCCCCTCAG CAACCAGAAA CATCACTTGT GCAGTCTCTT 
AAAACGGCTG CAAAAATCTT GAGTACACTT CAAACAAAAT TTAAGCATCA TGTATTGATA 
TACGAGAAAC CTTCTCTTCA GCAGAAAGCA CTGGCTTCAA TTCCCCTCCA GCAACTGAAA 
GGGAAAGCTC AGAAAAAGCT AACACAAGCT ACAAGACTGG ACAAAGGTGC ACATGTGAAT 
GAAGAGGACT TCTTGTTGTT GGAACTCTTG GACTGGTTTA AGAAGGATTT TTTTCATTGG 
GTAAACGCAC TTCCTTGCAG CAGGTGTGGT GGGGAGACAG TGCCTCAAAG TGAATATCTG 
TTGCCTACTG AAGGCGATCT AAGATGGAAT GCTAGTCGAG TGGAAAATCA TTACTGCAAC 
CAGTGTCAGA TTTGTAATAG ATTTCCAAGA TATAATGACC CTGAAAAACT TCTGGAAACC 
AGACGTGGAC GCTGTGGCGA GTGGGCTAAT TGTTTTACAC TGTGTTGCAG AGCTGTGGGG 
TTTGAAGCTA GATACGTTTG GGACTGTACA GATCATCTGT GGACTGAAGT ATATTCTTCA 
TCTCAGAAAA GATGGCTTCA CTGTGATCCT TGTGAAAATG TCTGTGATAA GCCTCTTATC 
TACGAAACTG GGTGGGGAAA GAAGCTCTCC TACATCTTTG CATTCTCCAA AGATGAGGTT 
GTTGATGTCA CCTGGAGATA CAGCTGTAAG CATGAAGAAG TGCTCTCTAG AAGGACTGCA 
CTCAGTGAAG CTACACTACG TGGAACAATT AATGCACTAA ATAGAATGAG GCAACGATCT 
TTGTCAGAAA ATAGAAGAAG AGAGCTCTTG GAAAGAACAA TTGTGGAGCT TGTTGAGTTT 
ATTTCTCCTA AAACACCTAA GCCTGGAGAA TATGGTGGAA GGACATCAGG TTCAATGGCT 
TGGCGAGTAG CCAGAGGTGA AATTGGTTCA GAGAAAAGAA GAGAAGTAAT TTTTGTTCCC 
TCTGGAAAAG AGAAGACATC TAAACTCTTC CACCTCATTT ACAATGTAGT AGAAGATAGT 
TATACACGGA TTTCCAATAA TAATGAAAAA ATAACTGGCT GGGAAGCAGG TGTTTGGAAA 
GCAGAGTCTG TCTGGAGAAA GGTTGAAACA GATTGGAAAA TGGTATATTT AGCCCGAAAA 
GAGGGGTCAT CCTCTGCTTC TGTCAGCTGG AAGTTTGAAT GCAAATCAGT TGGTCTGAAA 
ATAGAAAACA TTTCAGTTAG GACAAGCAGT CAGACATTTC ATAGTGGAAG AATAAAGTGG 
AGACTTTACT CTCCAACAGC AGAAATTGCT TTGATGGGAG ACAAAAATCT TTGCTCCTGT 
TCAGATTTTT CTGGTGCAAC GGAAGTTGTT TTGGAAGCAA TTCTGAATGG AGGGGATGGT 
GAAAGTGCAT GGCAACACAC ACAGCTGTTT AGAGACAGCT TAACAGGATG TGGAGAAAAT 
TGCTTGGAGA TAATTATAAA ACTCACTGAC CTCTGA