MIB2 cDNA ORF clone, Cuculus canorus(common cuckoo)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIB2
Entrez Gene ID	104055605
Full Name	mindbomb E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Cuculus canorus(common cuckoo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009556747.1	XP_009555042.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_009556746.1	XP_009555041.1	E3 ubiquitin-protein ligase MIB2 isoform X1

Pathways from BioSystems
Gene Ontology

The following MIB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu41145	XM_009556746.1 Latest version!	Cuculus canorus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCu41146	XM_009556747.1 Latest version!	Cuculus canorus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIB2 ( XM_009556746.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009555041.1 Cuculus canorus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCu41145
Clone ID Related Accession (Same CDS sequence)	XM_009556746.1
Accession Version	XM_009556746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009244235.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGG ACCCCTATGC CAGCATGCAG GTTGGGATGC GAGTAGTTCG TGGAGTGGAC 
TGGAAGTGGG GCAACCAGGA CAGTGGTGAA GGGAATGTTG GGACTGTGGT TGAGATTGGT 
CGGACAGGCA GCCCAACCAC TCCAGATAAG ACTGTGGTCG TGCAGTGGGA TCAAGGCAAC 
AGAACCAATT ATCGGACTGG ATTCCAAGGG GCTTATGACC TTCTTCTGTA CGATAACGCA 
CAAATAGGTG TCCGTCACCC TAACATCATC TGTGACTGTT GCAAGAAGCA TGGTATACGG 
GGAATGCGGT GGAAATGCAA AATGTGTTTT GACTATGACT TGTGCACGCA GTGTTACATG 
AACAACAAGC ACGATCTGTC TCACTCCTTC GAGCGCTATG AGACAGCACA CTCGCAACCA 
GTCCTGGTGA GTCCTCGACA GAATTTGACT CGCATCACCT TAAAAGGGAC TTTCCAGGGG 
GCTAAAGTGG TCCGTGGCCC CGACTGGGAG TGGGGTAACC AGGACGGTGG TGAGGGTAAA 
ACTGGACGTG TGGTTGATAT ACGTGGCTGG GATGTAGAGA CAGGACGCAG TGTGGCCAGC 
GTCATGTGGT CTGATGGTAC CACGAACGTC TACAGAGTGG GACACAAGGG AAAAGTGGAC 
CTGAAATGTA CTGTGGAGGC GTCTGGTGGC TTTTACTACA AAGAGCATCT CCCAAAATTA 
GGAAAACCAG CTGAACTACA GAGGAAGGAA AGCACGGACA GGCACCCATT CCAGCACGGG 
GACAAAGTGA AATGCCTACT GGACATCGAC ATCTTGCGGG AGATGCAGGA GGGCCATGGT 
GGCTGGAATC CGAAAATGGC TGAGTTCATT GGCCAGACAG GGACTGTGCA CAGAATCACG 
GACAGAGGGG ACGTGAGGGT CCAGTTCAAC AGCGAAACCC GCTGGACTTT CCATCCTGGA 
GCTCTGACTA AGCTCAACAC CTTTTGGGTT GGTGATGTTG TCCGGGTGAT AGATGATATG 
GAAACAGTGA AGCGATTCCA AGCTGGTCAT GGGGAGTGGA CAGATGAAAT GGCACCTACC 
CTAGGGCACA TCGGCAAAGT GATCAAGGTC TACGGGGATG GAGATTTGCG AGTGTCGGTT 
GGAGGGCAGT CCTGGACGTT CAACCCAGCT TGCCTGACAG CCTATCAGAG AGATGAGGAA 
GCAAACCTCA TGACAACGGA GAATGCAAAG GAACCAAAAA GTACTTTGGT TACTGTATTG 
GAGAAACTGC TGTCCCAGAA GACAGAGTCG GAGCACGCAG GCTGCCTGGT CATTTGTGCA 
GCGCTCAACA ACGCAGCTAA AGTTCGGGAG ATCCTTCAAA AATACCCAGA TAAGGTCGAC 
GCAAAGAACC AGGGCAGAAC TGCCCTGCAG ATCGCCTCTT ATCAGGGGCA CTTGGATGTT 
GTGAAGACTT TGCTGCAGGC ACATGCCAGT GTCAACCTGA GGGATGAAGA GGGGGATACA 
GCACTGCACT ACGCAGCTTT TGGGAACCAA GCTGAGGTTG CCCGTGTGCT TATTGCTAAA 
GGAGCCAGTG CAAACCTGCT AAATAATGCA AAGTGCACAG CGTTGTACGT TGCCGTCAGC 
CAAGGATTCA CAGAAGTGGT GCGGGCCCTC TGCGAGCTCA ACTGTGATGT CAACCTCCCA 
GATTCCCAGG GAGACACCCC CCTGCATTAT GCTATCACTG AAGATTACAA AGTCGTTATC 
GAGATTCTCA CTGAAGTACC CAACATAGAC TTCACTGTCC AGAATTGCCA AGGCTTCAAC 
CTGCTCCATT TTTCTGCTCT AAAAGGCAAC AAATTAGCCA TACAAAAGAT TCTAGCAAGA 
GCTCGGCAGC TGGTTGACTC CAAAAAGGAA GATGGCTTCA CTGCCCTGCA CCTTGCTGCT 
CTCAATAATC ACAAAGAAGT GGCAGAAATT CTCATAAAAG AGGGTCGCTG TGATGTCAAC 
CTCAAGAACA ACCGTAACCA GACACCGCTG CACCTGGCTG TCATCCAGGG ACACGTAGGG 
ATGGTGCAAC TGCTGGTCAG CGAAGGCAGT GATGTCAATG CTGAAGATGA AGACGGGGAT 
ACAGCCATGC ACATCGCCTT GGAACGGCAG CAGCTCATGT CCGTCATGAT GGAGAAGCGT 
GAAGGGGAGA TGGGATTGTC CCTCCTTTCC AAGCTGCAGG CTTCTGGATT TCTTGGAAAC 
ATAGAGCTGA ATGTTGGAAC TGCAATTGCA TGTTACTTAG CACAAGAAGG AGCAGATATT 
AATTATGCAA ACCATCGGGG AAAGTCTCCT TTGGACCTCA TTACAGATGG AAGGATTGTC 
CAGATGATCA AAGACTTCTC ACAGAAATTC AGGGAACAAC AGGTTTCTTC CGAGAGCTCA 
ACTATCACCT GCAGCCTTCG CCGTGTGCAC ACCACCCCCA ACACAATGAC CAACCTTAGC 
GTCTCCAGTG TGGCTGTCCC CACAGAGTGC CTGGTCTGCT CAGAGCTGGC CTTGCTCATC 
CACTTCTTCC CATGCCAGCA CAGCATTGTG TGTGAAGAAT GCTCCAGGAG AATGAAGAAG 
TGCATCAAAT GTCAAGTGAC AATAACCAAA AAACTAAAAC AAGACAGCAC CGAGGTGGAG 
TGCAGCCCAA GCTCTGAATC CACTGATCAG AGGAAACTCA TGGAGGAGCT GCAGAACCGC 
TACCGGCAGA TGGAAGAGCG CATCACCTGC CCCATTTGCA TTGATGACCA AATCAAACTG 
GTCTTCCAGT GTGGACATGG CTCTTGCCCA GACTGCAGCA CAGCCCTTAC CGTCTGCCCC 
ATCTGCCGGC AGGCTATCCG GGAGAGGATT CAGATCTTTG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009555041.1
CDS	105..2969
Translation	MDADPYASMQVGMRVVRGVDWKWGNQDSGEGNVGTVVEIGRTGSPTTPDKTVVVQWDQGNRTNYRTGFQGAYDLLLYDNAQIGVRHPNIICDCCKKHGIRGMRWKCKMCFDYDLCTQCYMNNKHDLSHSFERYETAHSQPVLVSPRQNLTRITLKGTFQGAKVVRGPDWEWGNQDGGEGKTGRVVDIRGWDVETGRSVASVMWSDGTTNVYRVGHKGKVDLKCTVEASGGFYYKEHLPKLGKPAELQRKESTDRHPFQHGDKVKCLLDIDILREMQEGHGGWNPKMAEFIGQTGTVHRITDRGDVRVQFNSETRWTFHPGALTKLNTFWVGDVVRVIDDMETVKRFQAGHGEWTDEMAPTLGHIGKVIKVYGDGDLRVSVGGQSWTFNPACLTAYQRDEEANLMTTENAKEPKSTLVTVLEKLLSQKTESEHAGCLVICAALNNAAKVREILQKYPDKVDAKNQGRTALQIASYQGHLDVVKTLLQAHASVNLRDEEGDTALHYAAFGNQAEVARVLIAKGASANLLNNAKCTALYVAVSQGFTEVVRALCELNCDVNLPDSQGDTPLHYAITEDYKVVIEILTEVPNIDFTVQNCQGFNLLHFSALKGNKLAIQKILARARQLVDSKKEDGFTALHLAALNNHKEVAEILIKEGRCDVNLKNNRNQTPLHLAVIQGHVGMVQLLVSEGSDVNAEDEDGDTAMHIALERQQLMSVMMEKREGEMGLSLLSKLQASGFLGNIELNVGTAIACYLAQEGADINYANHRGKSPLDLITDGRIVQMIKDFSQKFREQQVSSESSTITCSLRRVHTTPNTMTNLSVSSVAVPTECLVCSELALLIHFFPCQHSIVCEECSRRMKKCIKCQVTITKKLKQDSTEVECSPSSESTDQRKLMEELQNRYRQMEERITCPICIDDQIKLVFQCGHGSCPDCSTALTVCPICRQAIRERIQIFV

Target ORF information:

RefSeq Version	XM_009556746.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009556746.1

ORF Insert Sequence:

ATGGACGCGG ACCCCTATGC CAGCATGCAG GTTGGGATGC GAGTAGTTCG TGGAGTGGAC 
TGGAAGTGGG GCAACCAGGA CAGTGGTGAA GGGAATGTTG GGACTGTGGT TGAGATTGGT 
CGGACAGGCA GCCCAACCAC TCCAGATAAG ACTGTGGTCG TGCAGTGGGA TCAAGGCAAC 
AGAACCAATT ATCGGACTGG ATTCCAAGGG GCTTATGACC TTCTTCTGTA CGATAACGCA 
CAAATAGGTG TCCGTCACCC TAACATCATC TGTGACTGTT GCAAGAAGCA TGGTATACGG 
GGAATGCGGT GGAAATGCAA AATGTGTTTT GACTATGACT TGTGCACGCA GTGTTACATG 
AACAACAAGC ACGATCTGTC TCACTCCTTC GAGCGCTATG AGACAGCACA CTCGCAACCA 
GTCCTGGTGA GTCCTCGACA GAATTTGACT CGCATCACCT TAAAAGGGAC TTTCCAGGGG 
GCTAAAGTGG TCCGTGGCCC CGACTGGGAG TGGGGTAACC AGGACGGTGG TGAGGGTAAA 
ACTGGACGTG TGGTTGATAT ACGTGGCTGG GATGTAGAGA CAGGACGCAG TGTGGCCAGC 
GTCATGTGGT CTGATGGTAC CACGAACGTC TACAGAGTGG GACACAAGGG AAAAGTGGAC 
CTGAAATGTA CTGTGGAGGC GTCTGGTGGC TTTTACTACA AAGAGCATCT CCCAAAATTA 
GGAAAACCAG CTGAACTACA GAGGAAGGAA AGCACGGACA GGCACCCATT CCAGCACGGG 
GACAAAGTGA AATGCCTACT GGACATCGAC ATCTTGCGGG AGATGCAGGA GGGCCATGGT 
GGCTGGAATC CGAAAATGGC TGAGTTCATT GGCCAGACAG GGACTGTGCA CAGAATCACG 
GACAGAGGGG ACGTGAGGGT CCAGTTCAAC AGCGAAACCC GCTGGACTTT CCATCCTGGA 
GCTCTGACTA AGCTCAACAC CTTTTGGGTT GGTGATGTTG TCCGGGTGAT AGATGATATG 
GAAACAGTGA AGCGATTCCA AGCTGGTCAT GGGGAGTGGA CAGATGAAAT GGCACCTACC 
CTAGGGCACA TCGGCAAAGT GATCAAGGTC TACGGGGATG GAGATTTGCG AGTGTCGGTT 
GGAGGGCAGT CCTGGACGTT CAACCCAGCT TGCCTGACAG CCTATCAGAG AGATGAGGAA 
GCAAACCTCA TGACAACGGA GAATGCAAAG GAACCAAAAA GTACTTTGGT TACTGTATTG 
GAGAAACTGC TGTCCCAGAA GACAGAGTCG GAGCACGCAG GCTGCCTGGT CATTTGTGCA 
GCGCTCAACA ACGCAGCTAA AGTTCGGGAG ATCCTTCAAA AATACCCAGA TAAGGTCGAC 
GCAAAGAACC AGGGCAGAAC TGCCCTGCAG ATCGCCTCTT ATCAGGGGCA CTTGGATGTT 
GTGAAGACTT TGCTGCAGGC ACATGCCAGT GTCAACCTGA GGGATGAAGA GGGGGATACA 
GCACTGCACT ACGCAGCTTT TGGGAACCAA GCTGAGGTTG CCCGTGTGCT TATTGCTAAA 
GGAGCCAGTG CAAACCTGCT AAATAATGCA AAGTGCACAG CGTTGTACGT TGCCGTCAGC 
CAAGGATTCA CAGAAGTGGT GCGGGCCCTC TGCGAGCTCA ACTGTGATGT CAACCTCCCA 
GATTCCCAGG GAGACACCCC CCTGCATTAT GCTATCACTG AAGATTACAA AGTCGTTATC 
GAGATTCTCA CTGAAGTACC CAACATAGAC TTCACTGTCC AGAATTGCCA AGGCTTCAAC 
CTGCTCCATT TTTCTGCTCT AAAAGGCAAC AAATTAGCCA TACAAAAGAT TCTAGCAAGA 
GCTCGGCAGC TGGTTGACTC CAAAAAGGAA GATGGCTTCA CTGCCCTGCA CCTTGCTGCT 
CTCAATAATC ACAAAGAAGT GGCAGAAATT CTCATAAAAG AGGGTCGCTG TGATGTCAAC 
CTCAAGAACA ACCGTAACCA GACACCGCTG CACCTGGCTG TCATCCAGGG ACACGTAGGG 
ATGGTGCAAC TGCTGGTCAG CGAAGGCAGT GATGTCAATG CTGAAGATGA AGACGGGGAT 
ACAGCCATGC ACATCGCCTT GGAACGGCAG CAGCTCATGT CCGTCATGAT GGAGAAGCGT 
GAAGGGGAGA TGGGATTGTC CCTCCTTTCC AAGCTGCAGG CTTCTGGATT TCTTGGAAAC 
ATAGAGCTGA ATGTTGGAAC TGCAATTGCA TGTTACTTAG CACAAGAAGG AGCAGATATT 
AATTATGCAA ACCATCGGGG AAAGTCTCCT TTGGACCTCA TTACAGATGG AAGGATTGTC 
CAGATGATCA AAGACTTCTC ACAGAAATTC AGGGAACAAC AGGTTTCTTC CGAGAGCTCA 
ACTATCACCT GCAGCCTTCG CCGTGTGCAC ACCACCCCCA ACACAATGAC CAACCTTAGC 
GTCTCCAGTG TGGCTGTCCC CACAGAGTGC CTGGTCTGCT CAGAGCTGGC CTTGCTCATC 
CACTTCTTCC CATGCCAGCA CAGCATTGTG TGTGAAGAAT GCTCCAGGAG AATGAAGAAG 
TGCATCAAAT GTCAAGTGAC AATAACCAAA AAACTAAAAC AAGACAGCAC CGAGGTGGAG 
TGCAGCCCAA GCTCTGAATC CACTGATCAG AGGAAACTCA TGGAGGAGCT GCAGAACCGC 
TACCGGCAGA TGGAAGAGCG CATCACCTGC CCCATTTGCA TTGATGACCA AATCAAACTG 
GTCTTCCAGT GTGGACATGG CTCTTGCCCA GACTGCAGCA CAGCCCTTAC CGTCTGCCCC 
ATCTGCCGGC AGGCTATCCG GGAGAGGATT CAGATCTTTG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_009556747.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009555042.1 Cuculus canorus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCu41146
Clone ID Related Accession (Same CDS sequence)	XM_009556747.1
Accession Version	XM_009556747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009244235.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGG ACCCCTATGC CAGCATGCAG GTTGGGATGC GAGTAGTTCG TGGAGTGGAC 
TGGAAGTGGG GCAACCAGGA CAGTGGTGAA GGGAATGTTG GGACTGTGGT TGAGATTGGT 
CGGACAGGCA GCCCAACCAC TCCAGATAAG ACTGTGGTCG TGCAGTGGGA TCAAGGCAAC 
AGAACCAATT ATCGGACTGG ATTCCAAGGG GCTTATGACC TTCTTCTGTA CGATAACGCA 
CAAATAGGTG TCCGTCACCC TAACATCATC TGTGACTGTT GCAAGAAGCA TGGTATACGG 
GGAATGCGGT GGAAATGCAA AATGTGTTTT GACTATGACT TGTGCACGCA GTGTTACATG 
AACAACAAGC ACGATCTGTC TCACTCCTTC GAGCGCTATG AGACAGCACA CTCGCAACCA 
GTCCTGGTGA GTCCTCGACA GAATTTGACT CGCATCACCT TAAAAGGGAC TTTCCAGGGG 
GCTAAAGTGG TCCGTGGCCC CGACTGGGAG TGGGGTAACC AGGACGGTGG TGAGGGTAAA 
ACTGGACGTG TGGTTGATAT ACGTGGCTGG GATGTAGAGA CAGGACGCAG TGTGGCCAGC 
GTCATGTGGT CTGATGGTAC CACGAACGTC TACAGAGTGG GACACAAGGG AAAAGTGGAC 
CTGAAATGTA CTGTGGAGGC GTCTGGTGGC TTTTACTACA AAGAGCATCT CCCAAAATTA 
GGAAAACCAG CTGAACTACA GAGGAAGGAA AGCACGGACA GGCACCCATT CCAGCACGGG 
GACAAAGTGA AATGCCTACT GGACATCGAC ATCTTGCGGG AGATGCAGGA GGGCCATGGT 
GGCTGGAATC CGAAAATGGC TGAGACAGGG ACTGTGCACA GAATCACGGA CAGAGGGGAC 
GTGAGGGTCC AGTTCAACAG CGAAACCCGC TGGACTTTCC ATCCTGGAGC TCTGACTAAG 
CTCAACACCT TTTGGGTTGG TGATGTTGTC CGGGTGATAG ATGATATGGA AACAGTGAAG 
CGATTCCAAG CTGGTCATGG GGAGTGGACA GATGAAATGG CACCTACCCT AGGGCACATC 
GGCAAAGTGA TCAAGGTCTA CGGGGATGGA GATTTGCGAG TGTCGGTTGG AGGGCAGTCC 
TGGACGTTCA ACCCAGCTTG CCTGACAGCC TATCAGAGAG ATGAGGAAGC AAACCTCATG 
ACAACGGAGA ATGCAAAGGA ACCAAAAAGT ACTTTGGTTA CTGTATTGGA GAAACTGCTG 
TCCCAGAAGA CAGAGTCGGA GCACGCAGGC TGCCTGGTCA TTTGTGCAGC GCTCAACAAC 
GCAGCTAAAG TTCGGGAGAT CCTTCAAAAA TACCCAGATA AGGTCGACGC AAAGAACCAG 
GGCAGAACTG CCCTGCAGAT CGCCTCTTAT CAGGGGCACT TGGATGTTGT GAAGACTTTG 
CTGCAGGCAC ATGCCAGTGT CAACCTGAGG GATGAAGAGG GGGATACAGC ACTGCACTAC 
GCAGCTTTTG GGAACCAAGC TGAGGTTGCC CGTGTGCTTA TTGCTAAAGG AGCCAGTGCA 
AACCTGCTAA ATAATGCAAA GTGCACAGCG TTGTACGTTG CCGTCAGCCA AGGATTCACA 
GAAGTGGTGC GGGCCCTCTG CGAGCTCAAC TGTGATGTCA ACCTCCCAGA TTCCCAGGGA 
GACACCCCCC TGCATTATGC TATCACTGAA GATTACAAAG TCGTTATCGA GATTCTCACT 
GAAGTACCCA ACATAGACTT CACTGTCCAG AATTGCCAAG GCTTCAACCT GCTCCATTTT 
TCTGCTCTAA AAGGCAACAA ATTAGCCATA CAAAAGATTC TAGCAAGAGC TCGGCAGCTG 
GTTGACTCCA AAAAGGAAGA TGGCTTCACT GCCCTGCACC TTGCTGCTCT CAATAATCAC 
AAAGAAGTGG CAGAAATTCT CATAAAAGAG GGTCGCTGTG ATGTCAACCT CAAGAACAAC 
CGTAACCAGA CACCGCTGCA CCTGGCTGTC ATCCAGGGAC ACGTAGGGAT GGTGCAACTG 
CTGGTCAGCG AAGGCAGTGA TGTCAATGCT GAAGATGAAG ACGGGGATAC AGCCATGCAC 
ATCGCCTTGG AACGGCAGCA GCTCATGTCC GTCATGATGG AGAAGCGTGA AGGGGAGATG 
GGATTGTCCC TCCTTTCCAA GCTGCAGGCT TCTGGATTTC TTGGAAACAT AGAGCTGAAT 
GTTGGAACTG CAATTGCATG TTACTTAGCA CAAGAAGGAG CAGATATTAA TTATGCAAAC 
CATCGGGGAA AGTCTCCTTT GGACCTCATT ACAGATGGAA GGATTGTCCA GATGATCAAA 
GACTTCTCAC AGAAATTCAG GGAACAACAG GTTTCTTCCG AGAGCTCAAC TATCACCTGC 
AGCCTTCGCC GTGTGCACAC CACCCCCAAC ACAATGACCA ACCTTAGCGT CTCCAGTGTG 
GCTGTCCCCA CAGAGTGCCT GGTCTGCTCA GAGCTGGCCT TGCTCATCCA CTTCTTCCCA 
TGCCAGCACA GCATTGTGTG TGAAGAATGC TCCAGGAGAA TGAAGAAGTG CATCAAATGT 
CAAGTGACAA TAACCAAAAA ACTAAAACAA GACAGCACCG AGGTGGAGTG CAGCCCAAGC 
TCTGAATCCA CTGATCAGAG GAAACTCATG GAGGAGCTGC AGAACCGCTA CCGGCAGATG 
GAAGAGCGCA TCACCTGCCC CATTTGCATT GATGACCAAA TCAAACTGGT CTTCCAGTGT 
GGACATGGCT CTTGCCCAGA CTGCAGCACA GCCCTTACCG TCTGCCCCAT CTGCCGGCAG 
GCTATCCGGG AGAGGATTCA GATCTTTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009555042.1
CDS	103..2955
Translation	MDADPYASMQVGMRVVRGVDWKWGNQDSGEGNVGTVVEIGRTGSPTTPDKTVVVQWDQGNRTNYRTGFQGAYDLLLYDNAQIGVRHPNIICDCCKKHGIRGMRWKCKMCFDYDLCTQCYMNNKHDLSHSFERYETAHSQPVLVSPRQNLTRITLKGTFQGAKVVRGPDWEWGNQDGGEGKTGRVVDIRGWDVETGRSVASVMWSDGTTNVYRVGHKGKVDLKCTVEASGGFYYKEHLPKLGKPAELQRKESTDRHPFQHGDKVKCLLDIDILREMQEGHGGWNPKMAETGTVHRITDRGDVRVQFNSETRWTFHPGALTKLNTFWVGDVVRVIDDMETVKRFQAGHGEWTDEMAPTLGHIGKVIKVYGDGDLRVSVGGQSWTFNPACLTAYQRDEEANLMTTENAKEPKSTLVTVLEKLLSQKTESEHAGCLVICAALNNAAKVREILQKYPDKVDAKNQGRTALQIASYQGHLDVVKTLLQAHASVNLRDEEGDTALHYAAFGNQAEVARVLIAKGASANLLNNAKCTALYVAVSQGFTEVVRALCELNCDVNLPDSQGDTPLHYAITEDYKVVIEILTEVPNIDFTVQNCQGFNLLHFSALKGNKLAIQKILARARQLVDSKKEDGFTALHLAALNNHKEVAEILIKEGRCDVNLKNNRNQTPLHLAVIQGHVGMVQLLVSEGSDVNAEDEDGDTAMHIALERQQLMSVMMEKREGEMGLSLLSKLQASGFLGNIELNVGTAIACYLAQEGADINYANHRGKSPLDLITDGRIVQMIKDFSQKFREQQVSSESSTITCSLRRVHTTPNTMTNLSVSSVAVPTECLVCSELALLIHFFPCQHSIVCEECSRRMKKCIKCQVTITKKLKQDSTEVECSPSSESTDQRKLMEELQNRYRQMEERITCPICIDDQIKLVFQCGHGSCPDCSTALTVCPICRQAIRERIQIFV

Target ORF information:

RefSeq Version	XM_009556747.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009556747.1

ORF Insert Sequence:

ATGGACGCGG ACCCCTATGC CAGCATGCAG GTTGGGATGC GAGTAGTTCG TGGAGTGGAC 
TGGAAGTGGG GCAACCAGGA CAGTGGTGAA GGGAATGTTG GGACTGTGGT TGAGATTGGT 
CGGACAGGCA GCCCAACCAC TCCAGATAAG ACTGTGGTCG TGCAGTGGGA TCAAGGCAAC 
AGAACCAATT ATCGGACTGG ATTCCAAGGG GCTTATGACC TTCTTCTGTA CGATAACGCA 
CAAATAGGTG TCCGTCACCC TAACATCATC TGTGACTGTT GCAAGAAGCA TGGTATACGG 
GGAATGCGGT GGAAATGCAA AATGTGTTTT GACTATGACT TGTGCACGCA GTGTTACATG 
AACAACAAGC ACGATCTGTC TCACTCCTTC GAGCGCTATG AGACAGCACA CTCGCAACCA 
GTCCTGGTGA GTCCTCGACA GAATTTGACT CGCATCACCT TAAAAGGGAC TTTCCAGGGG 
GCTAAAGTGG TCCGTGGCCC CGACTGGGAG TGGGGTAACC AGGACGGTGG TGAGGGTAAA 
ACTGGACGTG TGGTTGATAT ACGTGGCTGG GATGTAGAGA CAGGACGCAG TGTGGCCAGC 
GTCATGTGGT CTGATGGTAC CACGAACGTC TACAGAGTGG GACACAAGGG AAAAGTGGAC 
CTGAAATGTA CTGTGGAGGC GTCTGGTGGC TTTTACTACA AAGAGCATCT CCCAAAATTA 
GGAAAACCAG CTGAACTACA GAGGAAGGAA AGCACGGACA GGCACCCATT CCAGCACGGG 
GACAAAGTGA AATGCCTACT GGACATCGAC ATCTTGCGGG AGATGCAGGA GGGCCATGGT 
GGCTGGAATC CGAAAATGGC TGAGACAGGG ACTGTGCACA GAATCACGGA CAGAGGGGAC 
GTGAGGGTCC AGTTCAACAG CGAAACCCGC TGGACTTTCC ATCCTGGAGC TCTGACTAAG 
CTCAACACCT TTTGGGTTGG TGATGTTGTC CGGGTGATAG ATGATATGGA AACAGTGAAG 
CGATTCCAAG CTGGTCATGG GGAGTGGACA GATGAAATGG CACCTACCCT AGGGCACATC 
GGCAAAGTGA TCAAGGTCTA CGGGGATGGA GATTTGCGAG TGTCGGTTGG AGGGCAGTCC 
TGGACGTTCA ACCCAGCTTG CCTGACAGCC TATCAGAGAG ATGAGGAAGC AAACCTCATG 
ACAACGGAGA ATGCAAAGGA ACCAAAAAGT ACTTTGGTTA CTGTATTGGA GAAACTGCTG 
TCCCAGAAGA CAGAGTCGGA GCACGCAGGC TGCCTGGTCA TTTGTGCAGC GCTCAACAAC 
GCAGCTAAAG TTCGGGAGAT CCTTCAAAAA TACCCAGATA AGGTCGACGC AAAGAACCAG 
GGCAGAACTG CCCTGCAGAT CGCCTCTTAT CAGGGGCACT TGGATGTTGT GAAGACTTTG 
CTGCAGGCAC ATGCCAGTGT CAACCTGAGG GATGAAGAGG GGGATACAGC ACTGCACTAC 
GCAGCTTTTG GGAACCAAGC TGAGGTTGCC CGTGTGCTTA TTGCTAAAGG AGCCAGTGCA 
AACCTGCTAA ATAATGCAAA GTGCACAGCG TTGTACGTTG CCGTCAGCCA AGGATTCACA 
GAAGTGGTGC GGGCCCTCTG CGAGCTCAAC TGTGATGTCA ACCTCCCAGA TTCCCAGGGA 
GACACCCCCC TGCATTATGC TATCACTGAA GATTACAAAG TCGTTATCGA GATTCTCACT 
GAAGTACCCA ACATAGACTT CACTGTCCAG AATTGCCAAG GCTTCAACCT GCTCCATTTT 
TCTGCTCTAA AAGGCAACAA ATTAGCCATA CAAAAGATTC TAGCAAGAGC TCGGCAGCTG 
GTTGACTCCA AAAAGGAAGA TGGCTTCACT GCCCTGCACC TTGCTGCTCT CAATAATCAC 
AAAGAAGTGG CAGAAATTCT CATAAAAGAG GGTCGCTGTG ATGTCAACCT CAAGAACAAC 
CGTAACCAGA CACCGCTGCA CCTGGCTGTC ATCCAGGGAC ACGTAGGGAT GGTGCAACTG 
CTGGTCAGCG AAGGCAGTGA TGTCAATGCT GAAGATGAAG ACGGGGATAC AGCCATGCAC 
ATCGCCTTGG AACGGCAGCA GCTCATGTCC GTCATGATGG AGAAGCGTGA AGGGGAGATG 
GGATTGTCCC TCCTTTCCAA GCTGCAGGCT TCTGGATTTC TTGGAAACAT AGAGCTGAAT 
GTTGGAACTG CAATTGCATG TTACTTAGCA CAAGAAGGAG CAGATATTAA TTATGCAAAC 
CATCGGGGAA AGTCTCCTTT GGACCTCATT ACAGATGGAA GGATTGTCCA GATGATCAAA 
GACTTCTCAC AGAAATTCAG GGAACAACAG GTTTCTTCCG AGAGCTCAAC TATCACCTGC 
AGCCTTCGCC GTGTGCACAC CACCCCCAAC ACAATGACCA ACCTTAGCGT CTCCAGTGTG 
GCTGTCCCCA CAGAGTGCCT GGTCTGCTCA GAGCTGGCCT TGCTCATCCA CTTCTTCCCA 
TGCCAGCACA GCATTGTGTG TGAAGAATGC TCCAGGAGAA TGAAGAAGTG CATCAAATGT 
CAAGTGACAA TAACCAAAAA ACTAAAACAA GACAGCACCG AGGTGGAGTG CAGCCCAAGC 
TCTGAATCCA CTGATCAGAG GAAACTCATG GAGGAGCTGC AGAACCGCTA CCGGCAGATG 
GAAGAGCGCA TCACCTGCCC CATTTGCATT GATGACCAAA TCAAACTGGT CTTCCAGTGT 
GGACATGGCT CTTGCCCAGA CTGCAGCACA GCCCTTACCG TCTGCCCCAT CTGCCGGCAG 
GCTATCCGGG AGAGGATTCA GATCTTTGTC TAA