Pde5a cDNA ORF clone, Cricetulus griseus(Chinese hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde5a
Entrez Gene ID	100755315
Full Name	phosphodiesterase 5A
Gene Type	protein-coding
Organism	Cricetulus griseus(Chinese hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007622972.2	XP_007621162.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
XM_007622973.1	XP_007621163.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
XM_007652015.2	XP_007650205.2	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
XM_027383913.1	XP_027239714.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
XM_027395645.1	XP_027251446.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
XM_027395646.1	XP_027251447.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3

Pathways from BioSystems
Gene Ontology

The following Pde5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCr220329	XM_027383913.1 Latest version!	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCr239042	XM_027395646.1 Latest version!	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCr28853	XM_007652015.2 Latest version!	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCr42193	XM_027395645.1 Latest version!	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCr42193	XM_007622972.2 Latest version!	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCr42194	XM_007622973.1 Latest version!	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde5a ( XM_027383913.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_027239714.1 Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr220329
Clone ID Related Accession (Same CDS sequence)	XM_027383913.1
Accession Version	XM_027383913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-17
Organism	Cricetulus griseus(Chinese hamster)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003615128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cricetulus griseus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGTTTTGC TCTGCAGAGA AATGGTCAAC GCATGGTTTT CCGAGAGAGT TCACAACATC 
CCCATATGCA AGGAAGGCAT CCGAGCCCAC ACGGAGTCAT GCTCCTGCTC CTCGCAGCAG 
AGTCCTCATG CAGACAATAC CACCCCTGGA GCCCCAGCCA GAAAAATCTC TGCCTCTGAA 
TTTGACCGGC CTCTGAGACC CATTGTTGTC AAGGATTCCG AGGGAACTGT GAGCTTTCTC 
TCTGACTCAG GAAAAAAGGA ACAGATGCCA CTAACCCCCC GAAGATTCGA TAGTGATGAA 
GGGGACCAGT GCTCCAGACT CTTGGAGTTA GTAAAAGATA TTTCCAGTCA TTTGGATGTC 
ACGGCCTTAT GTCACAAAAT TTTCTTGCAT ATCCACGGAC TCATCTCCGC CGATCGCTAT 
TCCCTATTCC TTGTTTGCGA GGACAGCTCC AAAGACAAAT TTCTTGTCAG CCGCCTCTTT 
GATGTCGCTG AAGGTTCGAC ACTGGAGGAA GCTTCCAATA ACTGTATCCG CTTGGAGTGG 
AACAAAGGCA TTGTGGGACA TGTGGCAGCT TTTGGTGAGC CGTTGAATAT CAAAGATGCC 
TATGAGGATC CTCGGTTCAA TGCTGAAGTC GACCAAATTA CAGGCTACAG GACACAGAGT 
ATCCTTTGTA TGCCAATTAA AAACCACAGA GAAGAGGTTG TTGGTGTAGC CCAGGCCATC 
AACAAGAAAT CAGGAAATGG TGGGACATTC ACTGAAAAGG ATGAAAAGGA CTTTGCTGCC 
TATTTGGCAT TTTGCGGTAT TGTCCTTCAC AATGCTCAGC TCTATGAAAC GTCACTGCTG 
GAGAATAAAA GAAATCAGGT ATTGCTTGAC CTTGCTAGCT TAATTTTCGA AGAGCAGCAG 
TCATTAGAAG TAATTCTGAA GAAAATAGCT GCCACTATCA TCTCCTTCAT GCAGGTGCAG 
AAGTGCACCA TATTCATCGT GGATGAAGAC TGCTCTGACT CTTTCTCTAG TGTGTTCCAC 
ATGGAGTGTG AGGAATTGGG AAAATCAGCT GATACTTTAA CAAGGGAGAA TGATAACACA 
ATGGAGCCAC TTAATATCCC TGATGTCGCT AAGGACAAAC GATTTCCCTG GACAAATGAA 
AACACAGGAC ATGTAAACAC ACACTGCATT GGAAGTTTGC TTTGCACACC TATAAAAAAC 
GGAAAGAAGA ACAAAGTCAT AGGAGTTTGC CAACTTGTCA ATAAGATGGA GGAGAATACT 
GGCAAGATTA AACCTTTCAA TCAAAATGAT GAACAGTTCC TGGAAGCTTT TGTTATCTTC 
TGTGGCTTGG GGATCCAGAA CACACAGATG TATGAAGCGG TGGAGAGAGC CATGGCCAAG 
CAAATGGTGA CATTAGAGGT CCTGTCCTAT CATGCATCGG CGGCAGAAGA AGAAACAAGA 
GAGCTCCAGG CTTTAGCGGC TGCTGTTGTG CCATCTGCCC AAACCCTTAA AATTACTGAC 
TTCAGCTTCA GTGACTTTGA GCTGTCTGAT GTGGAAACAG CACTGTGTAC AATTCGGATG 
TTCACTGACC TCAACCTTGT GCAGAACTTC CAGATGAAGC ATGAGGTTCT CTGCAGATGG 
ATTTTAAGTG TTAAGAAGAA TTACCGGAAG AATGTTGCCT ATCACAATTG GAGGCATGCC 
TTTAACACAG CACAGTGCAT GTTTGCTGCT CTGAAAGCAG GCAAGATTCA GAACAAGTTG 
ACGGATCTAG AGACACTTGC ATTGCTGATT GCTGCTTTAA GCCATGATTT GGATCATCGT 
GGTGTGAACA ACTCATACAT ACAGCGAAGT GAACACCCGC TGGCCCAGCT GTATTGCCAC 
TCCATCATGG AACACCATCA TTTTGATCAG TGCTTGATGA TTCTAAATAG CCCAGGCAAC 
CAGATTCTCA GTGGCCTCTC CATTGAAGAA TATAAGACCA CACTGAAAAT AATCAAGCAA 
GCAATTTTAG CTACAGACCT AGCACTGTAC ATTAAGAGAC GAGGAGAATT TTTTGAACTT 
ATAAGAAAAA ATCAATTCAG TTTTGAAGAT CCTCTTCAAA AGGAACTATT TCTAGCCATG 
CTGATGACTG CCTGTGATCT CTCTGCGATT ACCAAGCCCT GGCCTGTTCA ACAACGGATA 
GCAGAACTCG TGGCAGCTGA ATTCTTCGAT CAAGGAGACA GGGAGAGAAA AGAACTCAAC 
ATTGAGCCTG CTGATCTAAT GAACAGGGAG AAGAAAAATA AAATCCCAAG TATGCAAGTT 
GGGTTCATAG ACGCCATCTG CTTGCAGCTG TATGAGGCCC TGACCCATGT TTCAGAGGAC 
TGTTTGCCCT TGCTGGATGG CTGCAAGAAG AACAGACAGA AATGGCAAGC CCTTGCAGAG 
CAGCAGGAGA AGACTCTCAT TAATGGGGAA AGCAGCCAGA CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027239714.1
CDS	11..2458
Translation	MVLLCREMVNAWFSERVHNIPICKEGIRAHTESCSCSSQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPRRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYRTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELGKSADTLTRENDNTMEPLNIPDVAKDKRFPWTNENTGHVNTHCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKPFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALAAAVVPSAQTLKITDFSFSDFELSDVETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPVQQRIAELVAAEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCKKNRQKWQALAEQQEKTLINGESSQTK

Target ORF information:

RefSeq Version	XM_027383913.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027383913.1

ORF Insert Sequence:

ATGGTTTTGC TCTGCAGAGA AATGGTCAAC GCATGGTTTT CCGAGAGAGT TCACAACATC 
CCCATATGCA AGGAAGGCAT CCGAGCCCAC ACGGAGTCAT GCTCCTGCTC CTCGCAGCAG 
AGTCCTCATG CAGACAATAC CACCCCTGGA GCCCCAGCCA GAAAAATCTC TGCCTCTGAA 
TTTGACCGGC CTCTGAGACC CATTGTTGTC AAGGATTCCG AGGGAACTGT GAGCTTTCTC 
TCTGACTCAG GAAAAAAGGA ACAGATGCCA CTAACCCCCC GAAGATTCGA TAGTGATGAA 
GGGGACCAGT GCTCCAGACT CTTGGAGTTA GTAAAAGATA TTTCCAGTCA TTTGGATGTC 
ACGGCCTTAT GTCACAAAAT TTTCTTGCAT ATCCACGGAC TCATCTCCGC CGATCGCTAT 
TCCCTATTCC TTGTTTGCGA GGACAGCTCC AAAGACAAAT TTCTTGTCAG CCGCCTCTTT 
GATGTCGCTG AAGGTTCGAC ACTGGAGGAA GCTTCCAATA ACTGTATCCG CTTGGAGTGG 
AACAAAGGCA TTGTGGGACA TGTGGCAGCT TTTGGTGAGC CGTTGAATAT CAAAGATGCC 
TATGAGGATC CTCGGTTCAA TGCTGAAGTC GACCAAATTA CAGGCTACAG GACACAGAGT 
ATCCTTTGTA TGCCAATTAA AAACCACAGA GAAGAGGTTG TTGGTGTAGC CCAGGCCATC 
AACAAGAAAT CAGGAAATGG TGGGACATTC ACTGAAAAGG ATGAAAAGGA CTTTGCTGCC 
TATTTGGCAT TTTGCGGTAT TGTCCTTCAC AATGCTCAGC TCTATGAAAC GTCACTGCTG 
GAGAATAAAA GAAATCAGGT ATTGCTTGAC CTTGCTAGCT TAATTTTCGA AGAGCAGCAG 
TCATTAGAAG TAATTCTGAA GAAAATAGCT GCCACTATCA TCTCCTTCAT GCAGGTGCAG 
AAGTGCACCA TATTCATCGT GGATGAAGAC TGCTCTGACT CTTTCTCTAG TGTGTTCCAC 
ATGGAGTGTG AGGAATTGGG AAAATCAGCT GATACTTTAA CAAGGGAGAA TGATAACACA 
ATGGAGCCAC TTAATATCCC TGATGTCGCT AAGGACAAAC GATTTCCCTG GACAAATGAA 
AACACAGGAC ATGTAAACAC ACACTGCATT GGAAGTTTGC TTTGCACACC TATAAAAAAC 
GGAAAGAAGA ACAAAGTCAT AGGAGTTTGC CAACTTGTCA ATAAGATGGA GGAGAATACT 
GGCAAGATTA AACCTTTCAA TCAAAATGAT GAACAGTTCC TGGAAGCTTT TGTTATCTTC 
TGTGGCTTGG GGATCCAGAA CACACAGATG TATGAAGCGG TGGAGAGAGC CATGGCCAAG 
CAAATGGTGA CATTAGAGGT CCTGTCCTAT CATGCATCGG CGGCAGAAGA AGAAACAAGA 
GAGCTCCAGG CTTTAGCGGC TGCTGTTGTG CCATCTGCCC AAACCCTTAA AATTACTGAC 
TTCAGCTTCA GTGACTTTGA GCTGTCTGAT GTGGAAACAG CACTGTGTAC AATTCGGATG 
TTCACTGACC TCAACCTTGT GCAGAACTTC CAGATGAAGC ATGAGGTTCT CTGCAGATGG 
ATTTTAAGTG TTAAGAAGAA TTACCGGAAG AATGTTGCCT ATCACAATTG GAGGCATGCC 
TTTAACACAG CACAGTGCAT GTTTGCTGCT CTGAAAGCAG GCAAGATTCA GAACAAGTTG 
ACGGATCTAG AGACACTTGC ATTGCTGATT GCTGCTTTAA GCCATGATTT GGATCATCGT 
GGTGTGAACA ACTCATACAT ACAGCGAAGT GAACACCCGC TGGCCCAGCT GTATTGCCAC 
TCCATCATGG AACACCATCA TTTTGATCAG TGCTTGATGA TTCTAAATAG CCCAGGCAAC 
CAGATTCTCA GTGGCCTCTC CATTGAAGAA TATAAGACCA CACTGAAAAT AATCAAGCAA 
GCAATTTTAG CTACAGACCT AGCACTGTAC ATTAAGAGAC GAGGAGAATT TTTTGAACTT 
ATAAGAAAAA ATCAATTCAG TTTTGAAGAT CCTCTTCAAA AGGAACTATT TCTAGCCATG 
CTGATGACTG CCTGTGATCT CTCTGCGATT ACCAAGCCCT GGCCTGTTCA ACAACGGATA 
GCAGAACTCG TGGCAGCTGA ATTCTTCGAT CAAGGAGACA GGGAGAGAAA AGAACTCAAC 
ATTGAGCCTG CTGATCTAAT GAACAGGGAG AAGAAAAATA AAATCCCAAG TATGCAAGTT 
GGGTTCATAG ACGCCATCTG CTTGCAGCTG TATGAGGCCC TGACCCATGT TTCAGAGGAC 
TGTTTGCCCT TGCTGGATGG CTGCAAGAAG AACAGACAGA AATGGCAAGC CCTTGCAGAG 
CAGCAGGAGA AGACTCTCAT TAATGGGGAA AGCAGCCAGA CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde5a ( XM_027395646.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_027251447.1 Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCr239042
Clone ID Related Accession (Same CDS sequence)	XM_027395646.1
Accession Version	XM_027395646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-17
Organism	Cricetulus griseus(Chinese hamster)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020822438.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cricetulus griseus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGAG CCGGCCCCAG CTCGGAACGG CCGAGGCAGC AGCGGGACCA GGACTGGGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTGTTAG AAAAGCCACC 
AGAGAAATGG TCAATGCATG GTTTTCCGAG AGAGTTCACA ACATCCCCGT ATGCAAGGAA 
GGCATCCGAG CCCACACGGA GTCATGCTCC TGCTCCTCGC AGCAGAGTCC TCATGCAGAC 
AATACCACCC CTGGAGCCCC AGCCAGAAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTG 
AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACTGTGAGCT TTCTCTCTGA CTCAGGAAAA 
AAGGAACAGA TGCCACTAAC CCCCCGAAGA TTCGATAGTG ATGAAGGGGA CCAGTGCTCC 
AGACTCTTGG AGTTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACGGC CTTATGTCAC 
AAAATTTTCT TGCATATCCA CGGACTCATC TCCGCCGATC GCTATTCCCT ATTCCTTGTT 
TGCGAGGACA GCTCCAAAGA CAAATTTCTT GTCAGCCGCC TCTTTGATGT CGCTGAAGGT 
TCGACACTGG AGGAAGCTTC CAATAACTGT ATCCGCTTGG AGTGGAACAA AGGCATTGTG 
GGACATGTGG CAGCTTTTGG TGAGCCGTTG AATATCAAAG ATGCCTATGA GGATCCTCGG 
TTCAATGCTG AAGTCGACCA AATTACAGGC TACAGGACAC AGAGTATCCT TTGTATGCCA 
ATTAAAAACC ACAGAGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAGGATGAA AAGGACTTTG CTGCCTATTT GGCATTTTGC 
GGTATTGTCC TTCACAATGC TCAGCTCTAT GAAACGTCAC TGCTGGAGAA TAAAAGAAAT 
CAGGTATTGC TTGACCTTGC TAGCTTAATT TTCGAAGAGC AGCAGTCATT AGAAGTAATT 
CTGAAGAAAA TAGCTGCCAC TATTATCTCC TTCATGCAGG TGCAGAAGTG CACCATATTC 
ATCGTGGATG AAGACTGCTC TGACTCTTTC TCTAGTGTGT TCCACATGGA GTGTGAGGAA 
TTGGGAAAAT CAGCTGATAC TTTAACAAGG GAGCATGATG CAAACAAAAT CAATTACATG 
TATGCTCAGT ATGTCAAGAA CACAATGGAG CCACTTAATA TCCCTGATGT CGCTAAGGAC 
AAACGATTTC CCTGGACAAA TGAAAACACA GGACATGTAA ACACACACTG CATTGGAAGT 
TTGCTTTGCA CACCTATAAA AAACGGAAAG AAGAACAAAG TCATAGGAGT TTGCCAACTT 
GTCAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAGCCTT TCAATCAAAA TGATGAACAG 
TTCCTGGAAG CTTTTGTTAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCGGTGGAGA GAGCCATGGC CAAGCAAATG GTGACATTAG AGGTCCTGTC CTATCATGCA 
TCGGCGGCAG AAGAAGAAAC AAGAGAGCTC CAGGCTTTAG CGAACAAGTT GACGGATCTA 
GAGACACTTG CATTGCTGAT TGCTGCTTTA AGCCATGATT TGGATCATCG TGGTGTGAAC 
AACTCATACA TACAGCGAAG TGAACACCCG CTGGCCCAGC TGTATTGCCA CTCCATCATG 
GAACACCATC ATTTTGATCA GTGCTTGATG ATTCTAAATA GCCCAGGCAA CCAGATTCTC 
AGTGGCCTCT CCATTGAAGA ATATAAGACC ACACTGAAAA TAATCAAGCA AGCAATTTTA 
GCTACAGACC TAGCACTGTA CATTAAGAGA CGAGGAGAAT TTTTTGAACT TATAAGAAAA 
AATCAATTCA GTTTTGAAGA TCCTCTTCAA AAGGAACTAT TTCTAGCCAT GCTGATGACT 
GCCTGTGATC TCTCTGCGAT TACCAAGCCC TGGCCTGTTC AACAACGGAT AGCAGAACTC 
GTGGCAGCTG AATTCTTCGA TCAAGGAGAC AGGGAGAGAA AAGAACTCAA CATTGAGCCT 
GCTGATCTAA TGAACAGGGA GAAGAAAAAT AAAATCCCAA GTATGCAAGT TGGGTTCATA 
GACGCCATCT GCTTGCAGCT GTATGAGGCC CTGACCCATG TTTCAGAGGA CTGTTTGCCC 
TTGCTGGATG GCTGCAAGAA GAACAGACAG AAATGGCAAG CCCTTGCAGA GCAGCAGGAG 
AAGACTCTCA TTAATGGGGA AAGCAGCCAG ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027251447.1
CDS	236..2554
Translation	MERAGPSSERPRQQRDQDWVEAWLDDHRDFTFSYFVRKATREMVNAWFSERVHNIPVCKEGIRAHTESCSCSSQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPRRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYRTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELGKSADTLTREHDANKINYMYAQYVKNTMEPLNIPDVAKDKRFPWTNENTGHVNTHCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKPFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALANKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPVQQRIAELVAAEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCKKNRQKWQALAEQQEKTLINGESSQTK

Target ORF information:

RefSeq Version	XM_027395646.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027395646.1

ORF Insert Sequence:

ATGGAACGAG CCGGCCCCAG CTCGGAACGG CCGAGGCAGC AGCGGGACCA GGACTGGGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTGTTAG AAAAGCCACC 
AGAGAAATGG TCAATGCATG GTTTTCCGAG AGAGTTCACA ACATCCCCGT ATGCAAGGAA 
GGCATCCGAG CCCACACGGA GTCATGCTCC TGCTCCTCGC AGCAGAGTCC TCATGCAGAC 
AATACCACCC CTGGAGCCCC AGCCAGAAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTG 
AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACTGTGAGCT TTCTCTCTGA CTCAGGAAAA 
AAGGAACAGA TGCCACTAAC CCCCCGAAGA TTCGATAGTG ATGAAGGGGA CCAGTGCTCC 
AGACTCTTGG AGTTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACGGC CTTATGTCAC 
AAAATTTTCT TGCATATCCA CGGACTCATC TCCGCCGATC GCTATTCCCT ATTCCTTGTT 
TGCGAGGACA GCTCCAAAGA CAAATTTCTT GTCAGCCGCC TCTTTGATGT CGCTGAAGGT 
TCGACACTGG AGGAAGCTTC CAATAACTGT ATCCGCTTGG AGTGGAACAA AGGCATTGTG 
GGACATGTGG CAGCTTTTGG TGAGCCGTTG AATATCAAAG ATGCCTATGA GGATCCTCGG 
TTCAATGCTG AAGTCGACCA AATTACAGGC TACAGGACAC AGAGTATCCT TTGTATGCCA 
ATTAAAAACC ACAGAGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAGGATGAA AAGGACTTTG CTGCCTATTT GGCATTTTGC 
GGTATTGTCC TTCACAATGC TCAGCTCTAT GAAACGTCAC TGCTGGAGAA TAAAAGAAAT 
CAGGTATTGC TTGACCTTGC TAGCTTAATT TTCGAAGAGC AGCAGTCATT AGAAGTAATT 
CTGAAGAAAA TAGCTGCCAC TATTATCTCC TTCATGCAGG TGCAGAAGTG CACCATATTC 
ATCGTGGATG AAGACTGCTC TGACTCTTTC TCTAGTGTGT TCCACATGGA GTGTGAGGAA 
TTGGGAAAAT CAGCTGATAC TTTAACAAGG GAGCATGATG CAAACAAAAT CAATTACATG 
TATGCTCAGT ATGTCAAGAA CACAATGGAG CCACTTAATA TCCCTGATGT CGCTAAGGAC 
AAACGATTTC CCTGGACAAA TGAAAACACA GGACATGTAA ACACACACTG CATTGGAAGT 
TTGCTTTGCA CACCTATAAA AAACGGAAAG AAGAACAAAG TCATAGGAGT TTGCCAACTT 
GTCAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAGCCTT TCAATCAAAA TGATGAACAG 
TTCCTGGAAG CTTTTGTTAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCGGTGGAGA GAGCCATGGC CAAGCAAATG GTGACATTAG AGGTCCTGTC CTATCATGCA 
TCGGCGGCAG AAGAAGAAAC AAGAGAGCTC CAGGCTTTAG CGAACAAGTT GACGGATCTA 
GAGACACTTG CATTGCTGAT TGCTGCTTTA AGCCATGATT TGGATCATCG TGGTGTGAAC 
AACTCATACA TACAGCGAAG TGAACACCCG CTGGCCCAGC TGTATTGCCA CTCCATCATG 
GAACACCATC ATTTTGATCA GTGCTTGATG ATTCTAAATA GCCCAGGCAA CCAGATTCTC 
AGTGGCCTCT CCATTGAAGA ATATAAGACC ACACTGAAAA TAATCAAGCA AGCAATTTTA 
GCTACAGACC TAGCACTGTA CATTAAGAGA CGAGGAGAAT TTTTTGAACT TATAAGAAAA 
AATCAATTCA GTTTTGAAGA TCCTCTTCAA AAGGAACTAT TTCTAGCCAT GCTGATGACT 
GCCTGTGATC TCTCTGCGAT TACCAAGCCC TGGCCTGTTC AACAACGGAT AGCAGAACTC 
GTGGCAGCTG AATTCTTCGA TCAAGGAGAC AGGGAGAGAA AAGAACTCAA CATTGAGCCT 
GCTGATCTAA TGAACAGGGA GAAGAAAAAT AAAATCCCAA GTATGCAAGT TGGGTTCATA 
GACGCCATCT GCTTGCAGCT GTATGAGGCC CTGACCCATG TTTCAGAGGA CTGTTTGCCC 
TTGCTGGATG GCTGCAAGAA GAACAGACAG AAATGGCAAG CCCTTGCAGA GCAGCAGGAG 
AAGACTCTCA TTAATGGGGA AAGCAGCCAG ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde5a ( XM_007652015.2 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_007650205.2 Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCr28853
Clone ID Related Accession (Same CDS sequence)	XM_007652015.2
Accession Version	XM_007652015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-26
Organism	Cricetulus griseus(Chinese hamster)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003615128.1) and transcript sequence (JI873193.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 27, 2016 this sequence version replaced XM_007652015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cricetulus griseus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 41 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGTTTTGC TCTGCAGAGA AATGGTCAAC GCATGGTTTT CCGAGAGAGT TCACAACATC 
CCCATATGCA AGGAAGGCAT CCGAGCCCAC ACGGAGTCAT GCTCCTGCTC CTCGCAGCAG 
AGTCCTCATG CAGACAATAC CACCCCTGGA GCCCCAGCCA GAAAAATCTC TGCCTCTGAA 
TTTGACCGGC CTCTGAGACC CATTGTTGTC AAGGATTCCG AGGGAACTGT GAGCTTTCTC 
TCTGACTCAG GAAAAAAGGA ACAGATGCCA CTAACCCCCC GAAGATTCGA TAGTGATGAA 
GGGGACCAGT GCTCCAGACT CTTGGAGTTA GTAAAAGATA TTTCCAGTCA TTTGGATGTC 
ACGGCCTTAT GTCACAAAAT TTTCTTGCAT ATCCACGGAC TCATCTCCGC CGATCGCTAT 
TCCCTATTCC TTGTTTGCGA GGACAGCTCC AAAGACAAAT TTCTTGTCAG CCGCCTCTTT 
GATGTCGCTG AAGGTTCGAC ACTGGAGGAA GCTTCCAATA ACTGTATCCG CTTGGAGTGG 
AACAAAGGCA TTGTGGGACA TGTGGCAGCT TTTGGTGAGC CGTTGAATAT CAAAGATGCC 
TATGAGGATC CTCGGTTCAA TGCTGAAGTC GACCAAATTA CAGGCTACAG GACACAGAGT 
ATCCTTTGTA TGCCAATTAA AAACCACAGA GAAGAGGTTG TTGGTGTAGC CCAGGCCATC 
AACAAGAAAT CAGGAAATGG TGGGACATTC ACTGAAAAGG ATGAAAAGGA CTTTGCTGCC 
TATTTGGCAT TTTGCGGTAT TGTCCTTCAC AATGCTCAGC TCTATGAAAC GTCACTGCTG 
GAGAATAAAA GAAATCAGGT ATTGCTTGAC CTTGCTAGCT TAATTTTCGA AGAGCAGCAG 
TCATTAGAAG TAATTCTGAA GAAAATAGCT GCCACTATCA TCTCCTTCAT GCAGGTGCAG 
AAGTGCACCA TATTCATCGT GGATGAAGAC TGCTCTGACT CTTTCTCTAG TGTGTTCCAC 
ATGGAGTGTG AGGAATTGGG AAAATCAGCT GATACTTTAA CAAGGGAGCA TGATGCAAAC 
AAAATCAATT ACATGTATGC TCAGTATGTC AAGAACACAA TGGAGCCACT TAATATCCCT 
GATGTCGCTA AGGACAAACG ATTTCCCTGG ACAAATGAAA ACACAGGACA TGTAAACACA 
CACTGCATTG GAAGTTTGCT TTGCACACCT ATAAAAAACG GAAAGAAGAA CAAAGTCATA 
GGAGTTTGCC AACTTGTCAA TAAGATGGAG GAGAATACTG GCAAGATTAA ACCTTTCAAT 
CAAAATGATG AACAGTTCCT GGAAGCTTTT GTTATCTTCT GTGGCTTGGG GATCCAGAAC 
ACACAGATGT ATGAAGCGGT GGAGAGAGCC ATGGCCAAGC AAATGGTGAC ATTAGAGGTC 
CTGTCCTATC ATGCATCGGC GGCAGAAGAA GAAACAAGAG AGCTCCAGGC TTTAGCGGCT 
GCTGTTGTGC CATCTGCCCA AACCCTTAAA ATTACTGACT TCAGCTTCAG TGACTTTGAG 
CTGTCTGATG TGGAAACAGC ACTGTGTACA ATTCGGATGT TCACTGACCT CAACCTTGTG 
CAGAACTTCC AGATGAAGCA TGAGGTTCTC TGCAGATGGA TTTTAAGTGT TAAGAAGAAT 
TACCGGAAGA ATGTTGCCTA TCACAATTGG AGGCATGCCT TTAACACAGC ACAGTGCATG 
TTTGCTGCTC TGAAAGCAGG CAAGATTCAG AACAAGTTGA CGGATCTAGA GACACTTGCA 
TTGCTGATTG CTGCTTTAAG CCATGATTTG GATCATCGTG GTGTGAACAA CTCATACATA 
CAGCGAAGTG AACACCCGCT GGCCCAGCTG TATTGCCACT CCATCATGGA ACACCATCAT 
TTTGATCAGT GCTTGATGAT TCTAAATAGC CCAGGCAACC AGATTCTCAG TGGCCTCTCC 
ATTGAAGAAT ATAAGACCAC ACTGAAAATA ATCAAGCAAG CAATTTTAGC TACAGACCTA 
GCACTGTACA TTAAGAGACG AGGAGAATTT TTTGAACTTA TAAGAAAAAA TCAATTCAGT 
TTTGAAGATC CTCTTCAAAA GGAACTATTT CTAGCCATGC TGATGACTGC CTGTGATCTC 
TCTGCGATTA CCAAGCCCTG GCCTGTTCAA CAACGGATAG CAGAACTCGT GGCAGCTGAA 
TTCTTCGATC AAGGAGACAG GGAGAGAAAA GAACTCAACA TTGAGCCTGC TGATCTAATG 
AACAGGGAGA AGAAAAATAA AATCCCAAGT ATGCAAGTTG GGTTCATAGA CGCCATCTGC 
TTGCAGCTGT ATGAGGCCCT GACCCATGTT TCAGAGGACT GTTTGCCCTT GCTGGATGGC 
TGCAAGAAGA ACAGACAGAA ATGGCAAGCC CTTGCAGAGC AGCAGGAGAA GACTCTCATT 
AATGGGGAAA GCAGCCAGAC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007650205.2
CDS	8..2494
Translation	MVLLCREMVNAWFSERVHNIPICKEGIRAHTESCSCSSQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPRRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYRTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELGKSADTLTREHDANKINYMYAQYVKNTMEPLNIPDVAKDKRFPWTNENTGHVNTHCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKPFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALAAAVVPSAQTLKITDFSFSDFELSDVETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPVQQRIAELVAAEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCKKNRQKWQALAEQQEKTLINGESSQTK

Target ORF information:

RefSeq Version	XM_007652015.2
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007652015.2

ORF Insert Sequence:

ATGGTTTTGC TCTGCAGAGA AATGGTCAAC GCATGGTTTT CCGAGAGAGT TCACAACATC 
CCCATATGCA AGGAAGGCAT CCGAGCCCAC ACGGAGTCAT GCTCCTGCTC CTCGCAGCAG 
AGTCCTCATG CAGACAATAC CACCCCTGGA GCCCCAGCCA GAAAAATCTC TGCCTCTGAA 
TTTGACCGGC CTCTGAGACC CATTGTTGTC AAGGATTCCG AGGGAACTGT GAGCTTTCTC 
TCTGACTCAG GAAAAAAGGA ACAGATGCCA CTAACCCCCC GAAGATTCGA TAGTGATGAA 
GGGGACCAGT GCTCCAGACT CTTGGAGTTA GTAAAAGATA TTTCCAGTCA TTTGGATGTC 
ACGGCCTTAT GTCACAAAAT TTTCTTGCAT ATCCACGGAC TCATCTCCGC CGATCGCTAT 
TCCCTATTCC TTGTTTGCGA GGACAGCTCC AAAGACAAAT TTCTTGTCAG CCGCCTCTTT 
GATGTCGCTG AAGGTTCGAC ACTGGAGGAA GCTTCCAATA ACTGTATCCG CTTGGAGTGG 
AACAAAGGCA TTGTGGGACA TGTGGCAGCT TTTGGTGAGC CGTTGAATAT CAAAGATGCC 
TATGAGGATC CTCGGTTCAA TGCTGAAGTC GACCAAATTA CAGGCTACAG GACACAGAGT 
ATCCTTTGTA TGCCAATTAA AAACCACAGA GAAGAGGTTG TTGGTGTAGC CCAGGCCATC 
AACAAGAAAT CAGGAAATGG TGGGACATTC ACTGAAAAGG ATGAAAAGGA CTTTGCTGCC 
TATTTGGCAT TTTGCGGTAT TGTCCTTCAC AATGCTCAGC TCTATGAAAC GTCACTGCTG 
GAGAATAAAA GAAATCAGGT ATTGCTTGAC CTTGCTAGCT TAATTTTCGA AGAGCAGCAG 
TCATTAGAAG TAATTCTGAA GAAAATAGCT GCCACTATCA TCTCCTTCAT GCAGGTGCAG 
AAGTGCACCA TATTCATCGT GGATGAAGAC TGCTCTGACT CTTTCTCTAG TGTGTTCCAC 
ATGGAGTGTG AGGAATTGGG AAAATCAGCT GATACTTTAA CAAGGGAGCA TGATGCAAAC 
AAAATCAATT ACATGTATGC TCAGTATGTC AAGAACACAA TGGAGCCACT TAATATCCCT 
GATGTCGCTA AGGACAAACG ATTTCCCTGG ACAAATGAAA ACACAGGACA TGTAAACACA 
CACTGCATTG GAAGTTTGCT TTGCACACCT ATAAAAAACG GAAAGAAGAA CAAAGTCATA 
GGAGTTTGCC AACTTGTCAA TAAGATGGAG GAGAATACTG GCAAGATTAA ACCTTTCAAT 
CAAAATGATG AACAGTTCCT GGAAGCTTTT GTTATCTTCT GTGGCTTGGG GATCCAGAAC 
ACACAGATGT ATGAAGCGGT GGAGAGAGCC ATGGCCAAGC AAATGGTGAC ATTAGAGGTC 
CTGTCCTATC ATGCATCGGC GGCAGAAGAA GAAACAAGAG AGCTCCAGGC TTTAGCGGCT 
GCTGTTGTGC CATCTGCCCA AACCCTTAAA ATTACTGACT TCAGCTTCAG TGACTTTGAG 
CTGTCTGATG TGGAAACAGC ACTGTGTACA ATTCGGATGT TCACTGACCT CAACCTTGTG 
CAGAACTTCC AGATGAAGCA TGAGGTTCTC TGCAGATGGA TTTTAAGTGT TAAGAAGAAT 
TACCGGAAGA ATGTTGCCTA TCACAATTGG AGGCATGCCT TTAACACAGC ACAGTGCATG 
TTTGCTGCTC TGAAAGCAGG CAAGATTCAG AACAAGTTGA CGGATCTAGA GACACTTGCA 
TTGCTGATTG CTGCTTTAAG CCATGATTTG GATCATCGTG GTGTGAACAA CTCATACATA 
CAGCGAAGTG AACACCCGCT GGCCCAGCTG TATTGCCACT CCATCATGGA ACACCATCAT 
TTTGATCAGT GCTTGATGAT TCTAAATAGC CCAGGCAACC AGATTCTCAG TGGCCTCTCC 
ATTGAAGAAT ATAAGACCAC ACTGAAAATA ATCAAGCAAG CAATTTTAGC TACAGACCTA 
GCACTGTACA TTAAGAGACG AGGAGAATTT TTTGAACTTA TAAGAAAAAA TCAATTCAGT 
TTTGAAGATC CTCTTCAAAA GGAACTATTT CTAGCCATGC TGATGACTGC CTGTGATCTC 
TCTGCGATTA CCAAGCCCTG GCCTGTTCAA CAACGGATAG CAGAACTCGT GGCAGCTGAA 
TTCTTCGATC AAGGAGACAG GGAGAGAAAA GAACTCAACA TTGAGCCTGC TGATCTAATG 
AACAGGGAGA AGAAAAATAA AATCCCAAGT ATGCAAGTTG GGTTCATAGA CGCCATCTGC 
TTGCAGCTGT ATGAGGCCCT GACCCATGTT TCAGAGGACT GTTTGCCCTT GCTGGATGGC 
TGCAAGAAGA ACAGACAGAA ATGGCAAGCC CTTGCAGAGC AGCAGGAGAA GACTCTCATT 
AATGGGGAAA GCAGCCAGAC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde5a ( XM_007622972.2 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_007621162.1 Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr42193
Clone ID Related Accession (Same CDS sequence)	XM_007622972.2 , XM_027395645.1
Accession Version	XM_007622972.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-26
Organism	Cricetulus griseus(Chinese hamster)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006879544.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 27, 2016 this sequence version replaced XM_007622972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cricetulus griseus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGAG CCGGCCCCAG CTCGGAACGG CCGAGGCAGC AGCGGGACCA GGACTGGGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTGTTAG AAAAGCCACC 
AGAGAAATGG TCAATGCATG GTTTTCCGAG AGAGTTCACA ACATCCCCGT ATGCAAGGAA 
GGCATCCGAG CCCACACGGA GTCATGCTCC TGCTCCTCGC AGCAGAGTCC TCATGCAGAC 
AATACCACCC CTGGAGCCCC AGCCAGAAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTG 
AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACTGTGAGCT TTCTCTCTGA CTCAGGAAAA 
AAGGAACAGA TGCCACTAAC CCCCCGAAGA TTCGATAGTG ATGAAGGGGA CCAGTGCTCC 
AGACTCTTGG AGTTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACGGC CTTATGTCAC 
AAAATTTTCT TGCATATCCA CGGACTCATC TCCGCCGATC GCTATTCCCT ATTCCTTGTT 
TGCGAGGACA GCTCCAAAGA CAAATTTCTT GTCAGCCGCC TCTTTGATGT CGCTGAAGGT 
TCGACACTGG AGGAAGCTTC CAATAACTGT ATCCGCTTGG AGTGGAACAA AGGCATTGTG 
GGACATGTGG CAGCTTTTGG TGAGCCGTTG AATATCAAAG ATGCCTATGA GGATCCTCGG 
TTCAATGCTG AAGTCGACCA AATTACAGGC TACAGGACAC AGAGTATCCT TTGTATGCCA 
ATTAAAAACC ACAGAGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAGGATGAA AAGGACTTTG CTGCCTATTT GGCATTTTGC 
GGTATTGTCC TTCACAATGC TCAGCTCTAT GAAACGTCAC TGCTGGAGAA TAAAAGAAAT 
CAGGTATTGC TTGACCTTGC TAGCTTAATT TTCGAAGAGC AGCAGTCATT AGAAGTAATT 
CTGAAGAAAA TAGCTGCCAC TATTATCTCC TTCATGCAGG TGCAGAAGTG CACCATATTC 
ATCGTGGATG AAGACTGCTC TGACTCTTTC TCTAGTGTGT TCCACATGGA GTGTGAGGAA 
TTGGGAAAAT CAGCTGATAC TTTAACAAGG GAGCATGATG CAAACAAAAT CAATTACATG 
TATGCTCAGT ATGTCAAGAA CACAATGGAG CCACTTAATA TCCCTGATGT CGCTAAGGAC 
AAACGATTTC CCTGGACAAA TGAAAACACA GGACATGTAA ACACACACTG CATTGGAAGT 
TTGCTTTGCA CACCTATAAA AAACGGAAAG AAGAACAAAG TCATAGGAGT TTGCCAACTT 
GTCAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAGCCTT TCAATCAAAA TGATGAACAG 
TTCCTGGAAG CTTTTGTTAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCGGTGGAGA GAGCCATGGC CAAGCAAATG GTGACATTAG AGGTCCTGTC CTATCATGCA 
TCGGCGGCAG AAGAAGAAAC AAGAGAGCTC CAGGCTTTAG CGGCTGCTGT TGTGCCATCT 
GCCCAAACCC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGATGTGGAA 
ACAGCACTGT GTACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAGCATGAGG TTCTCTGCAG ATGGATTTTA AGTGTTAAGA AGAATTACCG GAAGAATGTT 
GCCTATCACA ATTGGAGGCA TGCCTTTAAC ACAGCACAGT GCATGTTTGC TGCTCTGAAA 
GCAGGCAAGA TTCAGAACAA GTTGACGGAT CTAGAGACAC TTGCATTGCT GATTGCTGCT 
TTAAGCCATG ATTTGGATCA TCGTGGTGTG AACAACTCAT ACATACAGCG AAGTGAACAC 
CCGCTGGCCC AGCTGTATTG CCACTCCATC ATGGAACACC ATCATTTTGA TCAGTGCTTG 
ATGATTCTAA ATAGCCCAGG CAACCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACACTGA AAATAATCAA GCAAGCAATT TTAGCTACAG ACCTAGCACT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAGTTTTGA AGATCCTCTT 
CAAAAGGAAC TATTTCTAGC CATGCTGATG ACTGCCTGTG ATCTCTCTGC GATTACCAAG 
CCCTGGCCTG TTCAACAACG GATAGCAGAA CTCGTGGCAG CTGAATTCTT CGATCAAGGA 
GACAGGGAGA GAAAAGAACT CAACATTGAG CCTGCTGATC TAATGAACAG GGAGAAGAAA 
AATAAAATCC CAAGTATGCA AGTTGGGTTC ATAGACGCCA TCTGCTTGCA GCTGTATGAG 
GCCCTGACCC ATGTTTCAGA GGACTGTTTG CCCTTGCTGG ATGGCTGCAA GAAGAACAGA 
CAGAAATGGC AAGCCCTTGC AGAGCAGCAG GAGAAGACTC TCATTAATGG GGAAAGCAGC 
CAGACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007621162.1
CDS	1..2592
Translation	MERAGPSSERPRQQRDQDWVEAWLDDHRDFTFSYFVRKATREMVNAWFSERVHNIPVCKEGIRAHTESCSCSSQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPRRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYRTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELGKSADTLTREHDANKINYMYAQYVKNTMEPLNIPDVAKDKRFPWTNENTGHVNTHCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKPFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALAAAVVPSAQTLKITDFSFSDFELSDVETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPVQQRIAELVAAEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCKKNRQKWQALAEQQEKTLINGESSQTK

Target ORF information:

RefSeq Version	XM_007622972.2
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007622972.2

ORF Insert Sequence:

ATGGAACGAG CCGGCCCCAG CTCGGAACGG CCGAGGCAGC AGCGGGACCA GGACTGGGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTGTTAG AAAAGCCACC 
AGAGAAATGG TCAATGCATG GTTTTCCGAG AGAGTTCACA ACATCCCCGT ATGCAAGGAA 
GGCATCCGAG CCCACACGGA GTCATGCTCC TGCTCCTCGC AGCAGAGTCC TCATGCAGAC 
AATACCACCC CTGGAGCCCC AGCCAGAAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTG 
AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACTGTGAGCT TTCTCTCTGA CTCAGGAAAA 
AAGGAACAGA TGCCACTAAC CCCCCGAAGA TTCGATAGTG ATGAAGGGGA CCAGTGCTCC 
AGACTCTTGG AGTTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACGGC CTTATGTCAC 
AAAATTTTCT TGCATATCCA CGGACTCATC TCCGCCGATC GCTATTCCCT ATTCCTTGTT 
TGCGAGGACA GCTCCAAAGA CAAATTTCTT GTCAGCCGCC TCTTTGATGT CGCTGAAGGT 
TCGACACTGG AGGAAGCTTC CAATAACTGT ATCCGCTTGG AGTGGAACAA AGGCATTGTG 
GGACATGTGG CAGCTTTTGG TGAGCCGTTG AATATCAAAG ATGCCTATGA GGATCCTCGG 
TTCAATGCTG AAGTCGACCA AATTACAGGC TACAGGACAC AGAGTATCCT TTGTATGCCA 
ATTAAAAACC ACAGAGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAGGATGAA AAGGACTTTG CTGCCTATTT GGCATTTTGC 
GGTATTGTCC TTCACAATGC TCAGCTCTAT GAAACGTCAC TGCTGGAGAA TAAAAGAAAT 
CAGGTATTGC TTGACCTTGC TAGCTTAATT TTCGAAGAGC AGCAGTCATT AGAAGTAATT 
CTGAAGAAAA TAGCTGCCAC TATTATCTCC TTCATGCAGG TGCAGAAGTG CACCATATTC 
ATCGTGGATG AAGACTGCTC TGACTCTTTC TCTAGTGTGT TCCACATGGA GTGTGAGGAA 
TTGGGAAAAT CAGCTGATAC TTTAACAAGG GAGCATGATG CAAACAAAAT CAATTACATG 
TATGCTCAGT ATGTCAAGAA CACAATGGAG CCACTTAATA TCCCTGATGT CGCTAAGGAC 
AAACGATTTC CCTGGACAAA TGAAAACACA GGACATGTAA ACACACACTG CATTGGAAGT 
TTGCTTTGCA CACCTATAAA AAACGGAAAG AAGAACAAAG TCATAGGAGT TTGCCAACTT 
GTCAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAGCCTT TCAATCAAAA TGATGAACAG 
TTCCTGGAAG CTTTTGTTAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCGGTGGAGA GAGCCATGGC CAAGCAAATG GTGACATTAG AGGTCCTGTC CTATCATGCA 
TCGGCGGCAG AAGAAGAAAC AAGAGAGCTC CAGGCTTTAG CGGCTGCTGT TGTGCCATCT 
GCCCAAACCC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGATGTGGAA 
ACAGCACTGT GTACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAGCATGAGG TTCTCTGCAG ATGGATTTTA AGTGTTAAGA AGAATTACCG GAAGAATGTT 
GCCTATCACA ATTGGAGGCA TGCCTTTAAC ACAGCACAGT GCATGTTTGC TGCTCTGAAA 
GCAGGCAAGA TTCAGAACAA GTTGACGGAT CTAGAGACAC TTGCATTGCT GATTGCTGCT 
TTAAGCCATG ATTTGGATCA TCGTGGTGTG AACAACTCAT ACATACAGCG AAGTGAACAC 
CCGCTGGCCC AGCTGTATTG CCACTCCATC ATGGAACACC ATCATTTTGA TCAGTGCTTG 
ATGATTCTAA ATAGCCCAGG CAACCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACACTGA AAATAATCAA GCAAGCAATT TTAGCTACAG ACCTAGCACT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAGTTTTGA AGATCCTCTT 
CAAAAGGAAC TATTTCTAGC CATGCTGATG ACTGCCTGTG ATCTCTCTGC GATTACCAAG 
CCCTGGCCTG TTCAACAACG GATAGCAGAA CTCGTGGCAG CTGAATTCTT CGATCAAGGA 
GACAGGGAGA GAAAAGAACT CAACATTGAG CCTGCTGATC TAATGAACAG GGAGAAGAAA 
AATAAAATCC CAAGTATGCA AGTTGGGTTC ATAGACGCCA TCTGCTTGCA GCTGTATGAG 
GCCCTGACCC ATGTTTCAGA GGACTGTTTG CCCTTGCTGG ATGGCTGCAA GAAGAACAGA 
CAGAAATGGC AAGCCCTTGC AGAGCAGCAG GAGAAGACTC TCATTAATGG GGAAAGCAGC 
CAGACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde5a ( XM_027395645.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_027251446.1 Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCr42193
Clone ID Related Accession (Same CDS sequence)	XM_007622972.2 , XM_027395645.1
Accession Version	XM_027395645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-17
Organism	Cricetulus griseus(Chinese hamster)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020822438.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cricetulus griseus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGAG CCGGCCCCAG CTCGGAACGG CCGAGGCAGC AGCGGGACCA GGACTGGGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTGTTAG AAAAGCCACC 
AGAGAAATGG TCAATGCATG GTTTTCCGAG AGAGTTCACA ACATCCCCGT ATGCAAGGAA 
GGCATCCGAG CCCACACGGA GTCATGCTCC TGCTCCTCGC AGCAGAGTCC TCATGCAGAC 
AATACCACCC CTGGAGCCCC AGCCAGAAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTG 
AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACTGTGAGCT TTCTCTCTGA CTCAGGAAAA 
AAGGAACAGA TGCCACTAAC CCCCCGAAGA TTCGATAGTG ATGAAGGGGA CCAGTGCTCC 
AGACTCTTGG AGTTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACGGC CTTATGTCAC 
AAAATTTTCT TGCATATCCA CGGACTCATC TCCGCCGATC GCTATTCCCT ATTCCTTGTT 
TGCGAGGACA GCTCCAAAGA CAAATTTCTT GTCAGCCGCC TCTTTGATGT CGCTGAAGGT 
TCGACACTGG AGGAAGCTTC CAATAACTGT ATCCGCTTGG AGTGGAACAA AGGCATTGTG 
GGACATGTGG CAGCTTTTGG TGAGCCGTTG AATATCAAAG ATGCCTATGA GGATCCTCGG 
TTCAATGCTG AAGTCGACCA AATTACAGGC TACAGGACAC AGAGTATCCT TTGTATGCCA 
ATTAAAAACC ACAGAGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAGGATGAA AAGGACTTTG CTGCCTATTT GGCATTTTGC 
GGTATTGTCC TTCACAATGC TCAGCTCTAT GAAACGTCAC TGCTGGAGAA TAAAAGAAAT 
CAGGTATTGC TTGACCTTGC TAGCTTAATT TTCGAAGAGC AGCAGTCATT AGAAGTAATT 
CTGAAGAAAA TAGCTGCCAC TATTATCTCC TTCATGCAGG TGCAGAAGTG CACCATATTC 
ATCGTGGATG AAGACTGCTC TGACTCTTTC TCTAGTGTGT TCCACATGGA GTGTGAGGAA 
TTGGGAAAAT CAGCTGATAC TTTAACAAGG GAGCATGATG CAAACAAAAT CAATTACATG 
TATGCTCAGT ATGTCAAGAA CACAATGGAG CCACTTAATA TCCCTGATGT CGCTAAGGAC 
AAACGATTTC CCTGGACAAA TGAAAACACA GGACATGTAA ACACACACTG CATTGGAAGT 
TTGCTTTGCA CACCTATAAA AAACGGAAAG AAGAACAAAG TCATAGGAGT TTGCCAACTT 
GTCAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAGCCTT TCAATCAAAA TGATGAACAG 
TTCCTGGAAG CTTTTGTTAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCGGTGGAGA GAGCCATGGC CAAGCAAATG GTGACATTAG AGGTCCTGTC CTATCATGCA 
TCGGCGGCAG AAGAAGAAAC AAGAGAGCTC CAGGCTTTAG CGGCTGCTGT TGTGCCATCT 
GCCCAAACCC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGATGTGGAA 
ACAGCACTGT GTACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAGCATGAGG TTCTCTGCAG ATGGATTTTA AGTGTTAAGA AGAATTACCG GAAGAATGTT 
GCCTATCACA ATTGGAGGCA TGCCTTTAAC ACAGCACAGT GCATGTTTGC TGCTCTGAAA 
GCAGGCAAGA TTCAGAACAA GTTGACGGAT CTAGAGACAC TTGCATTGCT GATTGCTGCT 
TTAAGCCATG ATTTGGATCA TCGTGGTGTG AACAACTCAT ACATACAGCG AAGTGAACAC 
CCGCTGGCCC AGCTGTATTG CCACTCCATC ATGGAACACC ATCATTTTGA TCAGTGCTTG 
ATGATTCTAA ATAGCCCAGG CAACCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACACTGA AAATAATCAA GCAAGCAATT TTAGCTACAG ACCTAGCACT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAGTTTTGA AGATCCTCTT 
CAAAAGGAAC TATTTCTAGC CATGCTGATG ACTGCCTGTG ATCTCTCTGC GATTACCAAG 
CCCTGGCCTG TTCAACAACG GATAGCAGAA CTCGTGGCAG CTGAATTCTT CGATCAAGGA 
GACAGGGAGA GAAAAGAACT CAACATTGAG CCTGCTGATC TAATGAACAG GGAGAAGAAA 
AATAAAATCC CAAGTATGCA AGTTGGGTTC ATAGACGCCA TCTGCTTGCA GCTGTATGAG 
GCCCTGACCC ATGTTTCAGA GGACTGTTTG CCCTTGCTGG ATGGCTGCAA GAAGAACAGA 
CAGAAATGGC AAGCCCTTGC AGAGCAGCAG GAGAAGACTC TCATTAATGG GGAAAGCAGC 
CAGACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027251446.1
CDS	1..2592
Translation	MERAGPSSERPRQQRDQDWVEAWLDDHRDFTFSYFVRKATREMVNAWFSERVHNIPVCKEGIRAHTESCSCSSQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPRRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYRTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELGKSADTLTREHDANKINYMYAQYVKNTMEPLNIPDVAKDKRFPWTNENTGHVNTHCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKPFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALAAAVVPSAQTLKITDFSFSDFELSDVETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPVQQRIAELVAAEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCKKNRQKWQALAEQQEKTLINGESSQTK

Target ORF information:

RefSeq Version	XM_027395645.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027395645.1

ORF Insert Sequence:

ATGGAACGAG CCGGCCCCAG CTCGGAACGG CCGAGGCAGC AGCGGGACCA GGACTGGGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTT ACCTTCTCTT ACTTTGTTAG AAAAGCCACC 
AGAGAAATGG TCAATGCATG GTTTTCCGAG AGAGTTCACA ACATCCCCGT ATGCAAGGAA 
GGCATCCGAG CCCACACGGA GTCATGCTCC TGCTCCTCGC AGCAGAGTCC TCATGCAGAC 
AATACCACCC CTGGAGCCCC AGCCAGAAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTG 
AGACCCATTG TTGTCAAGGA TTCCGAGGGA ACTGTGAGCT TTCTCTCTGA CTCAGGAAAA 
AAGGAACAGA TGCCACTAAC CCCCCGAAGA TTCGATAGTG ATGAAGGGGA CCAGTGCTCC 
AGACTCTTGG AGTTAGTAAA AGATATTTCC AGTCATTTGG ATGTCACGGC CTTATGTCAC 
AAAATTTTCT TGCATATCCA CGGACTCATC TCCGCCGATC GCTATTCCCT ATTCCTTGTT 
TGCGAGGACA GCTCCAAAGA CAAATTTCTT GTCAGCCGCC TCTTTGATGT CGCTGAAGGT 
TCGACACTGG AGGAAGCTTC CAATAACTGT ATCCGCTTGG AGTGGAACAA AGGCATTGTG 
GGACATGTGG CAGCTTTTGG TGAGCCGTTG AATATCAAAG ATGCCTATGA GGATCCTCGG 
TTCAATGCTG AAGTCGACCA AATTACAGGC TACAGGACAC AGAGTATCCT TTGTATGCCA 
ATTAAAAACC ACAGAGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAGGATGAA AAGGACTTTG CTGCCTATTT GGCATTTTGC 
GGTATTGTCC TTCACAATGC TCAGCTCTAT GAAACGTCAC TGCTGGAGAA TAAAAGAAAT 
CAGGTATTGC TTGACCTTGC TAGCTTAATT TTCGAAGAGC AGCAGTCATT AGAAGTAATT 
CTGAAGAAAA TAGCTGCCAC TATTATCTCC TTCATGCAGG TGCAGAAGTG CACCATATTC 
ATCGTGGATG AAGACTGCTC TGACTCTTTC TCTAGTGTGT TCCACATGGA GTGTGAGGAA 
TTGGGAAAAT CAGCTGATAC TTTAACAAGG GAGCATGATG CAAACAAAAT CAATTACATG 
TATGCTCAGT ATGTCAAGAA CACAATGGAG CCACTTAATA TCCCTGATGT CGCTAAGGAC 
AAACGATTTC CCTGGACAAA TGAAAACACA GGACATGTAA ACACACACTG CATTGGAAGT 
TTGCTTTGCA CACCTATAAA AAACGGAAAG AAGAACAAAG TCATAGGAGT TTGCCAACTT 
GTCAATAAGA TGGAGGAGAA TACTGGCAAG ATTAAGCCTT TCAATCAAAA TGATGAACAG 
TTCCTGGAAG CTTTTGTTAT CTTCTGTGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCGGTGGAGA GAGCCATGGC CAAGCAAATG GTGACATTAG AGGTCCTGTC CTATCATGCA 
TCGGCGGCAG AAGAAGAAAC AAGAGAGCTC CAGGCTTTAG CGGCTGCTGT TGTGCCATCT 
GCCCAAACCC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGATGTGGAA 
ACAGCACTGT GTACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAGCATGAGG TTCTCTGCAG ATGGATTTTA AGTGTTAAGA AGAATTACCG GAAGAATGTT 
GCCTATCACA ATTGGAGGCA TGCCTTTAAC ACAGCACAGT GCATGTTTGC TGCTCTGAAA 
GCAGGCAAGA TTCAGAACAA GTTGACGGAT CTAGAGACAC TTGCATTGCT GATTGCTGCT 
TTAAGCCATG ATTTGGATCA TCGTGGTGTG AACAACTCAT ACATACAGCG AAGTGAACAC 
CCGCTGGCCC AGCTGTATTG CCACTCCATC ATGGAACACC ATCATTTTGA TCAGTGCTTG 
ATGATTCTAA ATAGCCCAGG CAACCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACACTGA AAATAATCAA GCAAGCAATT TTAGCTACAG ACCTAGCACT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAGTTTTGA AGATCCTCTT 
CAAAAGGAAC TATTTCTAGC CATGCTGATG ACTGCCTGTG ATCTCTCTGC GATTACCAAG 
CCCTGGCCTG TTCAACAACG GATAGCAGAA CTCGTGGCAG CTGAATTCTT CGATCAAGGA 
GACAGGGAGA GAAAAGAACT CAACATTGAG CCTGCTGATC TAATGAACAG GGAGAAGAAA 
AATAAAATCC CAAGTATGCA AGTTGGGTTC ATAGACGCCA TCTGCTTGCA GCTGTATGAG 
GCCCTGACCC ATGTTTCAGA GGACTGTTTG CCCTTGCTGG ATGGCTGCAA GAAGAACAGA 
CAGAAATGGC AAGCCCTTGC AGAGCAGCAG GAGAAGACTC TCATTAATGG GGAAAGCAGC 
CAGACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde5a ( XM_007622973.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> XP_007621163.1 Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X3, mRNA.

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CloneID	OCr42194
Clone ID Related Accession (Same CDS sequence)	XM_007622973.1
Accession Version	XM_007622973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-26
Organism	Cricetulus griseus(Chinese hamster)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006879544.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cricetulus griseus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCCT TTGGAGACAA AACGAGAGAA ATGGTCAATG CATGGTTTTC CGAGAGAGTT 
CACAACATCC CCGTATGCAA GGAAGGCATC CGAGCCCACA CGGAGTCATG CTCCTGCTCC 
TCGCAGCAGA GTCCTCATGC AGACAATACC ACCCCTGGAG CCCCAGCCAG AAAAATCTCT 
GCCTCTGAAT TTGACCGGCC TCTGAGACCC ATTGTTGTCA AGGATTCCGA GGGAACTGTG 
AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAGATGCCAC TAACCCCCCG AAGATTCGAT 
AGTGATGAAG GGGACCAGTG CTCCAGACTC TTGGAGTTAG TAAAAGATAT TTCCAGTCAT 
TTGGATGTCA CGGCCTTATG TCACAAAATT TTCTTGCATA TCCACGGACT CATCTCCGCC 
GATCGCTATT CCCTATTCCT TGTTTGCGAG GACAGCTCCA AAGACAAATT TCTTGTCAGC 
CGCCTCTTTG ATGTCGCTGA AGGTTCGACA CTGGAGGAAG CTTCCAATAA CTGTATCCGC 
TTGGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGTGAGCC GTTGAATATC 
AAAGATGCCT ATGAGGATCC TCGGTTCAAT GCTGAAGTCG ACCAAATTAC AGGCTACAGG 
ACACAGAGTA TCCTTTGTAT GCCAATTAAA AACCACAGAG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACATTCA CTGAAAAGGA TGAAAAGGAC 
TTTGCTGCCT ATTTGGCATT TTGCGGTATT GTCCTTCACA ATGCTCAGCT CTATGAAACG 
TCACTGCTGG AGAATAAAAG AAATCAGGTA TTGCTTGACC TTGCTAGCTT AATTTTCGAA 
GAGCAGCAGT CATTAGAAGT AATTCTGAAG AAAATAGCTG CCACTATTAT CTCCTTCATG 
CAGGTGCAGA AGTGCACCAT ATTCATCGTG GATGAAGACT GCTCTGACTC TTTCTCTAGT 
GTGTTCCACA TGGAGTGTGA GGAATTGGGA AAATCAGCTG ATACTTTAAC AAGGGAGCAT 
GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AGAACACAAT GGAGCCACTT 
AATATCCCTG ATGTCGCTAA GGACAAACGA TTTCCCTGGA CAAATGAAAA CACAGGACAT 
GTAAACACAC ACTGCATTGG AAGTTTGCTT TGCACACCTA TAAAAAACGG AAAGAAGAAC 
AAAGTCATAG GAGTTTGCCA ACTTGTCAAT AAGATGGAGG AGAATACTGG CAAGATTAAG 
CCTTTCAATC AAAATGATGA ACAGTTCCTG GAAGCTTTTG TTATCTTCTG TGGCTTGGGG 
ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA AATGGTGACA 
TTAGAGGTCC TGTCCTATCA TGCATCGGCG GCAGAAGAAG AAACAAGAGA GCTCCAGGCT 
TTAGCGGCTG CTGTTGTGCC ATCTGCCCAA ACCCTTAAAA TTACTGACTT CAGCTTCAGT 
GACTTTGAGC TGTCTGATGT GGAAACAGCA CTGTGTACAA TTCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAGCAT GAGGTTCTCT GCAGATGGAT TTTAAGTGTT 
AAGAAGAATT ACCGGAAGAA TGTTGCCTAT CACAATTGGA GGCATGCCTT TAACACAGCA 
CAGTGCATGT TTGCTGCTCT GAAAGCAGGC AAGATTCAGA ACAAGTTGAC GGATCTAGAG 
ACACTTGCAT TGCTGATTGC TGCTTTAAGC CATGATTTGG ATCATCGTGG TGTGAACAAC 
TCATACATAC AGCGAAGTGA ACACCCGCTG GCCCAGCTGT ATTGCCACTC CATCATGGAA 
CACCATCATT TTGATCAGTG CTTGATGATT CTAAATAGCC CAGGCAACCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCT 
ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT 
CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAACTATTTC TAGCCATGCT GATGACTGCC 
TGTGATCTCT CTGCGATTAC CAAGCCCTGG CCTGTTCAAC AACGGATAGC AGAACTCGTG 
GCAGCTGAAT TCTTCGATCA AGGAGACAGG GAGAGAAAAG AACTCAACAT TGAGCCTGCT 
GATCTAATGA ACAGGGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAC 
GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCATGTTT CAGAGGACTG TTTGCCCTTG 
CTGGATGGCT GCAAGAAGAA CAGACAGAAA TGGCAAGCCC TTGCAGAGCA GCAGGAGAAG 
ACTCTCATTA ATGGGGAAAG CAGCCAGACC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007621163.1
CDS	102..2597
Translation	MLPFGDKTREMVNAWFSERVHNIPVCKEGIRAHTESCSCSSQQSPHADNTTPGAPARKISASEFDRPLRPIVVKDSEGTVSFLSDSGKKEQMPLTPRRFDSDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYRTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELGKSADTLTREHDANKINYMYAQYVKNTMEPLNIPDVAKDKRFPWTNENTGHVNTHCIGSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKIKPFNQNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQALAAAVVPSAQTLKITDFSFSDFELSDVETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFSFEDPLQKELFLAMLMTACDLSAITKPWPVQQRIAELVAAEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCLPLLDGCKKNRQKWQALAEQQEKTLINGESSQTK

Target ORF information:

RefSeq Version	XM_007622973.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus phosphodiesterase 5A (Pde5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007622973.1

ORF Insert Sequence:

ATGTTGCCCT TTGGAGACAA AACGAGAGAA ATGGTCAATG CATGGTTTTC CGAGAGAGTT 
CACAACATCC CCGTATGCAA GGAAGGCATC CGAGCCCACA CGGAGTCATG CTCCTGCTCC 
TCGCAGCAGA GTCCTCATGC AGACAATACC ACCCCTGGAG CCCCAGCCAG AAAAATCTCT 
GCCTCTGAAT TTGACCGGCC TCTGAGACCC ATTGTTGTCA AGGATTCCGA GGGAACTGTG 
AGCTTTCTCT CTGACTCAGG AAAAAAGGAA CAGATGCCAC TAACCCCCCG AAGATTCGAT 
AGTGATGAAG GGGACCAGTG CTCCAGACTC TTGGAGTTAG TAAAAGATAT TTCCAGTCAT 
TTGGATGTCA CGGCCTTATG TCACAAAATT TTCTTGCATA TCCACGGACT CATCTCCGCC 
GATCGCTATT CCCTATTCCT TGTTTGCGAG GACAGCTCCA AAGACAAATT TCTTGTCAGC 
CGCCTCTTTG ATGTCGCTGA AGGTTCGACA CTGGAGGAAG CTTCCAATAA CTGTATCCGC 
TTGGAGTGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCTT TTGGTGAGCC GTTGAATATC 
AAAGATGCCT ATGAGGATCC TCGGTTCAAT GCTGAAGTCG ACCAAATTAC AGGCTACAGG 
ACACAGAGTA TCCTTTGTAT GCCAATTAAA AACCACAGAG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACATTCA CTGAAAAGGA TGAAAAGGAC 
TTTGCTGCCT ATTTGGCATT TTGCGGTATT GTCCTTCACA ATGCTCAGCT CTATGAAACG 
TCACTGCTGG AGAATAAAAG AAATCAGGTA TTGCTTGACC TTGCTAGCTT AATTTTCGAA 
GAGCAGCAGT CATTAGAAGT AATTCTGAAG AAAATAGCTG CCACTATTAT CTCCTTCATG 
CAGGTGCAGA AGTGCACCAT ATTCATCGTG GATGAAGACT GCTCTGACTC TTTCTCTAGT 
GTGTTCCACA TGGAGTGTGA GGAATTGGGA AAATCAGCTG ATACTTTAAC AAGGGAGCAT 
GATGCAAACA AAATCAATTA CATGTATGCT CAGTATGTCA AGAACACAAT GGAGCCACTT 
AATATCCCTG ATGTCGCTAA GGACAAACGA TTTCCCTGGA CAAATGAAAA CACAGGACAT 
GTAAACACAC ACTGCATTGG AAGTTTGCTT TGCACACCTA TAAAAAACGG AAAGAAGAAC 
AAAGTCATAG GAGTTTGCCA ACTTGTCAAT AAGATGGAGG AGAATACTGG CAAGATTAAG 
CCTTTCAATC AAAATGATGA ACAGTTCCTG GAAGCTTTTG TTATCTTCTG TGGCTTGGGG 
ATCCAGAACA CACAGATGTA TGAAGCGGTG GAGAGAGCCA TGGCCAAGCA AATGGTGACA 
TTAGAGGTCC TGTCCTATCA TGCATCGGCG GCAGAAGAAG AAACAAGAGA GCTCCAGGCT 
TTAGCGGCTG CTGTTGTGCC ATCTGCCCAA ACCCTTAAAA TTACTGACTT CAGCTTCAGT 
GACTTTGAGC TGTCTGATGT GGAAACAGCA CTGTGTACAA TTCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAGCAT GAGGTTCTCT GCAGATGGAT TTTAAGTGTT 
AAGAAGAATT ACCGGAAGAA TGTTGCCTAT CACAATTGGA GGCATGCCTT TAACACAGCA 
CAGTGCATGT TTGCTGCTCT GAAAGCAGGC AAGATTCAGA ACAAGTTGAC GGATCTAGAG 
ACACTTGCAT TGCTGATTGC TGCTTTAAGC CATGATTTGG ATCATCGTGG TGTGAACAAC 
TCATACATAC AGCGAAGTGA ACACCCGCTG GCCCAGCTGT ATTGCCACTC CATCATGGAA 
CACCATCATT TTGATCAGTG CTTGATGATT CTAAATAGCC CAGGCAACCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAATA TAAGACCACA CTGAAAATAA TCAAGCAAGC AATTTTAGCT 
ACAGACCTAG CACTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT 
CAATTCAGTT TTGAAGATCC TCTTCAAAAG GAACTATTTC TAGCCATGCT GATGACTGCC 
TGTGATCTCT CTGCGATTAC CAAGCCCTGG CCTGTTCAAC AACGGATAGC AGAACTCGTG 
GCAGCTGAAT TCTTCGATCA AGGAGACAGG GAGAGAAAAG AACTCAACAT TGAGCCTGCT 
GATCTAATGA ACAGGGAGAA GAAAAATAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAC 
GCCATCTGCT TGCAGCTGTA TGAGGCCCTG ACCCATGTTT CAGAGGACTG TTTGCCCTTG 
CTGGATGGCT GCAAGAAGAA CAGACAGAAA TGGCAAGCCC TTGCAGAGCA GCAGGAGAAG 
ACTCTCATTA ATGGGGAAAG CAGCCAGACC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Pde5a cDNA ORF clone, Cricetulus griseus(Chinese hamster)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム