Oaz2 cDNA ORF clone, Cricetulus griseus(Chinese hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Oaz2
Entrez Gene ID	100752199
Full Name	ornithine decarboxylase antizyme 2
Gene Type	protein-coding
Organism	Cricetulus griseus(Chinese hamster)
Summary	The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamine in cells. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis pathway; thus, completing the auto-regulatory circuit. This gene encodes antizyme 2, the second member of the antizyme family. Like antizyme 1, antizyme 2 has broad tissue distribution, inhibits ODC activity and polyamine uptake, and stimulates ODC degradation in vivo, however, it fails to promote ODC degradation in vitro. Antizyme 2 is expressed at lower levels than antizyme 1, but is evolutionary more conserved, suggesting it likely has an important biological role. Studies also show different subcellular localization of antizymes 1 and 2, indicating specific function for each antizyme in discrete compartments of the cell. [provided by RefSeq, Aug 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001291906.1	NP_001278835.1	ornithine decarboxylase antizyme 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing.
Becker J, Hackl M, Rupp O, Jakobi T, Schneider J, Szczepanowski R, Bekel T, Borth N, Goesmann A, Grillari J, Kaltschmidt C, Noll T, P?hler A, Tauch A, Brinkrolf K
Journal of biotechnology156(3)227-35(2011 Dec)

Ribosomal frameshifting in decoding antizyme mRNAs from yeast and protists to humans: close to 300 cases reveal remarkable diversity despite underlying conservation.
Ivanov IP, Atkins JF
Nucleic acids research35(6)1842-58(2007)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Oaz2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Oaz2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr126280	NM_001291906.1 Latest version!	Cricetulus griseus ornithine decarboxylase antizyme 2 (Oaz2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Oaz2 ( NM_001291906.1 ) cDNA ORF clone, Cricetulus griseus(Chinese hamster) -> NP_001278835.1 Cricetulus griseus ornithine decarboxylase antizyme 2 (Oaz2), mRNA.

Price & Availability↑

CloneID	OCr126280
Clone ID Related Accession (Same CDS sequence)	NM_001291906.1
Accession Version	NM_001291906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Cricetulus griseus(Chinese hamster)
Product	ornithine decarboxylase antizyme 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AFTD01170565.1 and JP061177.1. On May 11, 2014 this sequence version replaced XM_003514493.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: JI879565.1, JP061177.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4681446, SAMEA4681447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## ribosomal slippage :: inferred from conservation ##RefSeq-Attributes-END##

ATGATACACA CCCAGGACAG TAGTATTTTG CCTTTGAGTA ACTGTCCCCA GCTCCAGTGC 
TGCAGGCACA TTGTTCCAGG GCCTCTGTGG TGCTCCGATG CCCCTCACCC ACTGTCGAAG 
ATCCCCGGTG GGCGAGGGGG CGGCAGGGAT CCTTCTCTCT CAGCTCTAAT ATATAAGGAC 
GAGAAGCTCA CTGTAACCCA GGACCTCCCT GTGAACGATG GGAAACCTCA CATCGTCCAT 
TTCCAGTATG AGGTCACCGA GGTGAAGGTC TCTTCCTGGG ATGCAGTCCT GTCCAGCCAG 
AGCCTGTTTA TAGAAATCCC AGATGGATTA TTAGCTGATG GGAGCAAAGA AGGATTGTTA 
GCACTGCTAG AGTTTGCTGA GGAGAAGATG AAAGTGAACT ACGTCTTCAT CTGCTTCAGG 
AAGGGCCGGG AAGACAGAGC TCCACTCCTG AAGACATTCA GCTTCTTGGG CTTTGAGATT 
GTACGTCCAG GTCATCCCTG TGTCCCCTCT CGGCCAGATG TGATGTTCAT GGTTTACCCC 
CTGGACCAGA ACTTGTCTGA TGAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278835.1
CDS	join(207..302,304..777)
Translation	MIHTQDSSILPLSNCPQLQCCRHIVPGPLWCSDAPHPLSKIPGGRGGGRDPSLSALIYKDEKLTVTQDLPVNDGKPHIVHFQYEVTEVKVSSWDAVLSSQSLFIEIPDGLLADGSKEGLLALLEFAEEKMKVNYVFICFRKGREDRAPLLKTFSFLGFEIVRPGHPCVPSRPDVMFMVYPLDQNLSDED

Target ORF information:

RefSeq Version	NM_001291906.1
Organism	Cricetulus griseus(Chinese hamster)
Definition	Cricetulus griseus ornithine decarboxylase antizyme 2 (Oaz2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291906.1

ORF Insert Sequence:

ATGATACACA CCCAGGACAG TAGTATTTTG CCTTTGAGTA ACTGTCCCCA GCTCCAGTGC 
TGCAGGCACA TTGTTCCAGG GCCTCTGTGG TGCTCCGATG CCCCTCACCC ACTGTCGAAG 
ATCCCCGGTG GGCGAGGGGG CGGCAGGGAT CCTTCTCTCT CAGCTCTAAT ATATAAGGAC 
GAGAAGCTCA CTGTAACCCA GGACCTCCCT GTGAACGATG GGAAACCTCA CATCGTCCAT 
TTCCAGTATG AGGTCACCGA GGTGAAGGTC TCTTCCTGGG ATGCAGTCCT GTCCAGCCAG 
AGCCTGTTTA TAGAAATCCC AGATGGATTA TTAGCTGATG GGAGCAAAGA AGGATTGTTA 
GCACTGCTAG AGTTTGCTGA GGAGAAGATG AAAGTGAACT ACGTCTTCAT CTGCTTCAGG 
AAGGGCCGGG AAGACAGAGC TCCACTCCTG AAGACATTCA GCTTCTTGGG CTTTGAGATT 
GTACGTCCAG GTCATCCCTG TGTCCCCTCT CGGCCAGATG TGATGTTCAT GGTTTACCCC 
CTGGACCAGA ACTTGTCTGA TGAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing.
Journal of biotechnology156(3)227-35(2011 Dec)
Becker J,Hackl M,Rupp O,Jakobi T,Schneider J,Szczepanowski R,Bekel T,Borth N,Goesmann A,Grillari J,Kaltschmidt C,Noll T,P?hler A,Tauch A,Brinkrolf K

Ribosomal frameshifting in decoding antizyme mRNAs from yeast and protists to humans: close to 300 cases reveal remarkable diversity despite underlying conservation.
Nucleic acids research35(6)1842-58(2007)
Ivanov IP,Atkins JF