LOC105345075 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105345075
Entrez Gene ID	105345075
Full Name	E3 ubiquitin-protein ligase RFWD2-like
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011453064.2	XP_011451366.1	E3 ubiquitin-protein ligase RFWD2-like

Pathways from BioSystems
Gene Ontology

The following LOC105345075 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105345075 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr112158	XM_011453064.2 Latest version!	Crassostrea gigas E3 ubiquitin-protein ligase RFWD2-like (LOC105345075), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105345075 ( XM_011453064.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011451366.1 Crassostrea gigas E3 ubiquitin-protein ligase RFWD2-like (LOC105345075), mRNA.

Price & Availability↑

CloneID	OCr112158
Clone ID Related Accession (Same CDS sequence)	XM_011453064.2
Accession Version	XM_011453064.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	E3 ubiquitin-protein ligase RFWD2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011936986.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011453064.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATC GGACTACTTT GCATCAACGC AGACGAAAAC GAGCCCAGCC TCCAGTCTTT 
AGTGGAATTT CTGGGTCATA CGAAGACAAA GACAGTGACT TCCTGTGTCC TATATGTTTT 
AACTTGATGG AAGAAGTACA TATGACTAAA TGTGGTCACT CATATTGCCA TAAATGCATA 
AAGGAAAGTC TTCAACAAAG CAACAGATGT CCAAAGTGCA CTTATGTTAT AGAGGGCATT 
GACCATGTCT TTCCAAACTT CATGTTGAAT GAGCTGGTGT TAAAATACAG GGGCAAACAG 
GAACAAAAGA AAATAAAACT TGAACAAAAG ACGTCTCCTT CAACAGAAAC TGTTGATATC 
TGTGATATGA TTTTGGAGGA CAACATTGAT CTCTGTGATG TGAACAAACT GCTAGAAGTC 
CTTAATGTGA AGAAGCAAAA GCTAGAAGGG GACATCAAGT TTGCACAGAA ATATATACTT 
AAAGAATTTC TTCAAGAAAT AAAAAAAAGA AAGCAAGGAA AATTAGAACA GTTAAAAAGG 
GAAATTTCTT TGATTGATGA CGATTTCGAA AAAGTGGATG GAATGCTTTC AGAACACAGA 
AGAAAGTATC CGTACATTCC AGATCATCTG GTGCCAAATG GGTTTGAGCC TACCATGACG 
GCAAGCACTG ATCCTGGCAG TCAAGACGGA TTCAATGGCA GCAAGAATGC TGGAAAACCC 
TGGTTACAGA CAACCATGGC CTCCAGAAGG AAAAGAGTCA GTCAGCATTT TGAGGATCTA 
GAGCAATGTT ACTTCTCCAT CAGACAGAAG GATGAGTATA ATGGAGAGGA GGGGTTAGAT 
GAGTTTACAG AATGTCTTTC AAAATTCACC AAGTTTAATT CATTCAGGTC ACTTGCCACT 
TTAAGTTATG CAAGTGACAT TTACAGTGGG TCCAGTATAG TCTCAAGCAT AGAGTTTGAC 
AGGGATTGTG ACTTCTTTGC CATAGCTGGT GTAACCAAAA AAATCAAGGT GTTTGAGTAT 
GGAACTGTGA TAAAGGATGC TGTTGATATA CATTATCCAG TGAATGAGAT GATCTGCAAT 
TCAAAAATTA GTTGTGTGAC TTGGAGTAGT TATCACAAAA ATGTCCTGGC AAGCTCAGAT 
TATGAAGGGA CAATTACACT GTGGGATGCA TTCACTGGCC AGAAATCTAA ACTCTTTCAG 
GAACATGAGA AAAGATGCTG GAGTGTGGAC TTTAACACAA TGGATCCCAA ACTGTTGGCA 
TCAGGATCAG ATGATGCTAA AGTGAAGCTT TGGTCTATCA ATGCAGAGAA CTCAATTGCT 
TGTCTAGAAG CCAAGGCAAA TGTTTGTTGT GTCAAATTCA ACCCTGAGAG TAGATATCAC 
TTAGCGTTTG GTTCTGCAGA TCATTTTGTG CATTACTATG ATCTGAGAAA CACTAAAGAG 
GCAGTGATGG TCTTCAAGGG GCACAGAAAG GCTGTGTCAT ACACCAAGTT CCTCAATACC 
ACAGAAATTG TGTCTGCATC AACAGACAGT CAGCTCAAAC TTTGGAATAT TGATAAATCA 
AATTCTTTGA GAACTTTTAA AGGACATATC AATGAGAAGA ATTTTGTAGG CTTAGCTACA 
GATGGGGATT ATATTGCTTG TGGAAGTGAA AACAATTCCC TATATGTGTA CTACAAGGGG 
CTTTCAAAAC AGCTGATGAC CTATAAGTTT GACACAGTGC GGAGTGTGCT GCAAGAAAAA 
GACAAGAAAG AAGATGAGGT CAATGAGTTT GTTAGTGCTG TTGCTTGGAG ACCGGGTTCA 
AATGTGGTTG TAGCAGCTAA CAGTCAAGGA ACAGTGAAGG TGCTAGAGTT GATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011451366.1
CDS	159..2015
Translation	MANRTTLHQRRRKRAQPPVFSGISGSYEDKDSDFLCPICFNLMEEVHMTKCGHSYCHKCIKESLQQSNRCPKCTYVIEGIDHVFPNFMLNELVLKYRGKQEQKKIKLEQKTSPSTETVDICDMILEDNIDLCDVNKLLEVLNVKKQKLEGDIKFAQKYILKEFLQEIKKRKQGKLEQLKREISLIDDDFEKVDGMLSEHRRKYPYIPDHLVPNGFEPTMTASTDPGSQDGFNGSKNAGKPWLQTTMASRRKRVSQHFEDLEQCYFSIRQKDEYNGEEGLDEFTECLSKFTKFNSFRSLATLSYASDIYSGSSIVSSIEFDRDCDFFAIAGVTKKIKVFEYGTVIKDAVDIHYPVNEMICNSKISCVTWSSYHKNVLASSDYEGTITLWDAFTGQKSKLFQEHEKRCWSVDFNTMDPKLLASGSDDAKVKLWSINAENSIACLEAKANVCCVKFNPESRYHLAFGSADHFVHYYDLRNTKEAVMVFKGHRKAVSYTKFLNTTEIVSASTDSQLKLWNIDKSNSLRTFKGHINEKNFVGLATDGDYIACGSENNSLYVYYKGLSKQLMTYKFDTVRSVLQEKDKKEDEVNEFVSAVAWRPGSNVVVAANSQGTVKVLELI

Target ORF information:

RefSeq Version	XM_011453064.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas E3 ubiquitin-protein ligase RFWD2-like (LOC105345075), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011453064.2

ORF Insert Sequence:

ATGGCAAATC GGACTACTTT GCATCAACGC AGACGAAAAC GAGCCCAGCC TCCAGTCTTT 
AGTGGAATTT CTGGGTCATA CGAAGACAAA GACAGTGACT TCCTGTGTCC TATATGTTTT 
AACTTGATGG AAGAAGTACA TATGACTAAA TGTGGTCACT CATATTGCCA TAAATGCATA 
AAGGAAAGTC TTCAACAAAG CAACAGATGT CCAAAGTGCA CTTATGTTAT AGAGGGCATT 
GACCATGTCT TTCCAAACTT CATGTTGAAT GAGCTGGTGT TAAAATACAG GGGCAAACAG 
GAACAAAAGA AAATAAAACT TGAACAAAAG ACGTCTCCTT CAACAGAAAC TGTTGATATC 
TGTGATATGA TTTTGGAGGA CAACATTGAT CTCTGTGATG TGAACAAACT GCTAGAAGTC 
CTTAATGTGA AGAAGCAAAA GCTAGAAGGG GACATCAAGT TTGCACAGAA ATATATACTT 
AAAGAATTTC TTCAAGAAAT AAAAAAAAGA AAGCAAGGAA AATTAGAACA GTTAAAAAGG 
GAAATTTCTT TGATTGATGA CGATTTCGAA AAAGTGGATG GAATGCTTTC AGAACACAGA 
AGAAAGTATC CGTACATTCC AGATCATCTG GTGCCAAATG GGTTTGAGCC TACCATGACG 
GCAAGCACTG ATCCTGGCAG TCAAGACGGA TTCAATGGCA GCAAGAATGC TGGAAAACCC 
TGGTTACAGA CAACCATGGC CTCCAGAAGG AAAAGAGTCA GTCAGCATTT TGAGGATCTA 
GAGCAATGTT ACTTCTCCAT CAGACAGAAG GATGAGTATA ATGGAGAGGA GGGGTTAGAT 
GAGTTTACAG AATGTCTTTC AAAATTCACC AAGTTTAATT CATTCAGGTC ACTTGCCACT 
TTAAGTTATG CAAGTGACAT TTACAGTGGG TCCAGTATAG TCTCAAGCAT AGAGTTTGAC 
AGGGATTGTG ACTTCTTTGC CATAGCTGGT GTAACCAAAA AAATCAAGGT GTTTGAGTAT 
GGAACTGTGA TAAAGGATGC TGTTGATATA CATTATCCAG TGAATGAGAT GATCTGCAAT 
TCAAAAATTA GTTGTGTGAC TTGGAGTAGT TATCACAAAA ATGTCCTGGC AAGCTCAGAT 
TATGAAGGGA CAATTACACT GTGGGATGCA TTCACTGGCC AGAAATCTAA ACTCTTTCAG 
GAACATGAGA AAAGATGCTG GAGTGTGGAC TTTAACACAA TGGATCCCAA ACTGTTGGCA 
TCAGGATCAG ATGATGCTAA AGTGAAGCTT TGGTCTATCA ATGCAGAGAA CTCAATTGCT 
TGTCTAGAAG CCAAGGCAAA TGTTTGTTGT GTCAAATTCA ACCCTGAGAG TAGATATCAC 
TTAGCGTTTG GTTCTGCAGA TCATTTTGTG CATTACTATG ATCTGAGAAA CACTAAAGAG 
GCAGTGATGG TCTTCAAGGG GCACAGAAAG GCTGTGTCAT ACACCAAGTT CCTCAATACC 
ACAGAAATTG TGTCTGCATC AACAGACAGT CAGCTCAAAC TTTGGAATAT TGATAAATCA 
AATTCTTTGA GAACTTTTAA AGGACATATC AATGAGAAGA ATTTTGTAGG CTTAGCTACA 
GATGGGGATT ATATTGCTTG TGGAAGTGAA AACAATTCCC TATATGTGTA CTACAAGGGG 
CTTTCAAAAC AGCTGATGAC CTATAAGTTT GACACAGTGC GGAGTGTGCT GCAAGAAAAA 
GACAAGAAAG AAGATGAGGT CAATGAGTTT GTTAGTGCTG TTGCTTGGAG ACCGGGTTCA 
AATGTGGTTG TAGCAGCTAA CAGTCAAGGA ACAGTGAAGG TGCTAGAGTT GATATAG