Btg1 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Btg1
Entrez Gene ID	105340066
Full Name	B-cell translocation gene 1, anti-proliferative
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001308892.1	NP_001295821.1	uncharacterized protein LOC105340066

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response.
Liu R, Cheng Q, Wang X, Chen H, Wang W, Zhang H, Wang L, Song L
Fish & shellfish immunology6168-78(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CgBTG1 mRNA is predominantly expressed in hemocytes, and its expression level in hemocytes is significantly up-regulated at 6 h post Vibrio splendidus stimulation. The apoptosis rate of oyster hemocytes is significantly decreased after CgBTG1 interfered by dsRNA.
Title: The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response.

The following Btg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Btg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCr126339	NM_001308892.1 Latest version!	Crassostrea gigas B-cell translocation gene 1, anti-proliferative (Btg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Btg1 ( NM_001308892.1 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> NP_001295821.1 Crassostrea gigas B-cell translocation gene 1, anti-proliferative (Btg1), mRNA.

Price & Availability↑

CloneID	OCr126339
Clone ID Related Accession (Same CDS sequence)	NM_001308892.1
Accession Version	NM_001308892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-22
Organism	Crassostrea gigas(Pacific oyster)
Product	uncharacterized protein LOC105340066
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from FJ009577.1. On May 16, 2015 this sequence version replaced XM_011445903.1. ##Evidence-Data-START## Transcript is intronless :: FJ009577.1 [ECO:0000345] ##Evidence-Data-END##

ATGAAAAACG AAGTTAAAAG TGCTGTAGAC TTCCTCGCAA ACATCTTGCG GACTTCTAAA 
CATGTAAGTG AACAACAGGT GCATATTTTT AAAGAGAATT TACAGAATTT ATTGAGTTCA 
AAATTCGAGA ATCACTGGTT TCCCTCCAAG CCAAACAAAG GCAGCGGCTA TCGATGCATT 
CGTATCAACC ACAAGATGGA CCCCTTACTT CTTCAGGCCG GCCACTCTTG TGGCCTAAAC 
GAGACCGTCA TATTTTCCAT CATTCCTAAT GAACTCACAA TTTGGGTGGA TCCATTCGAC 
GTGTCCTACC GCATAGGAGA AAATGGCAGC ATTGGGGTGC TTTTCGAGTC TGATAACACA 
TCTTTCAATG ACAACTCATC CTCCTCCATG TCATCAACTT CATCATCCTC ATCATTGTCC 
AGCGGAAGCG AATCCCCCTC ACCCATGTCC ATGATGTCTT TCTCAGCCAA TTCATGCAAA 
GGACAGTTCA TGAGCGAATT TCCAAGGGAT ATGGGTCTCA AGCAATTCGC CGCCTATGTG 
TACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295821.1
CDS	1..549
Translation	MKNEVKSAVDFLANILRTSKHVSEQQVHIFKENLQNLLSSKFENHWFPSKPNKGSGYRCIRINHKMDPLLLQAGHSCGLNETVIFSIIPNELTIWVDPFDVSYRIGENGSIGVLFESDNTSFNDNSSSSMSSTSSSSSLSSGSESPSPMSMMSFSANSCKGQFMSEFPRDMGLKQFAAYVYS

Target ORF information:

RefSeq Version	NM_001308892.1
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas B-cell translocation gene 1, anti-proliferative (Btg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308892.1

ORF Insert Sequence:

ATGAAAAACG AAGTTAAAAG TGCTGTAGAC TTCCTCGCAA ACATCTTGCG GACTTCTAAA 
CATGTAAGTG AACAACAGGT GCATATTTTT AAAGAGAATT TACAGAATTT ATTGAGTTCA 
AAATTCGAGA ATCACTGGTT TCCCTCCAAG CCAAACAAAG GCAGCGGCTA TCGATGCATT 
CGTATCAACC ACAAGATGGA CCCCTTACTT CTTCAGGCCG GCCACTCTTG TGGCCTAAAC 
GAGACCGTCA TATTTTCCAT CATTCCTAAT GAACTCACAA TTTGGGTGGA TCCATTCGAC 
GTGTCCTACC GCATAGGAGA AAATGGCAGC ATTGGGGTGC TTTTCGAGTC TGATAACACA 
TCTTTCAATG ACAACTCATC CTCCTCCATG TCATCAACTT CATCATCCTC ATCATTGTCC 
AGCGGAAGCG AATCCCCCTC ACCCATGTCC ATGATGTCTT TCTCAGCCAA TTCATGCAAA 
GGACAGTTCA TGAGCGAATT TCCAAGGGAT ATGGGTCTCA AGCAATTCGC CGCCTATGTG 
TACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response.
Fish & shellfish immunology6168-78(2017 Feb)
Liu R,Cheng Q,Wang X,Chen H,Wang W,Zhang H,Wang L,Song L