EIF2AK3 cDNA ORF clone, Condylura cristata(star-nosed mole)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF2AK3
Entrez Gene ID	101629330
Full Name	eukaryotic translation initiation factor 2 alpha kinase 3
Gene Type	protein-coding
Organism	Condylura cristata(star-nosed mole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004691619.2	XP_004691676.1	eukaryotic translation initiation factor 2-alpha kinase 3 isoform X1
XM_012731596.1	XP_012587050.1	eukaryotic translation initiation factor 2-alpha kinase 3 isoform X2

Pathways from BioSystems
Gene Ontology

The following EIF2AK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2AK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo243400	XM_004691619.2 Latest version!	Condylura cristata eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCo243401	XM_012731596.1 Latest version!	Condylura cristata eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF2AK3 ( XM_004691619.2 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_004691676.1 Condylura cristata eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCo243400
Clone ID Related Accession (Same CDS sequence)	XM_004691619.2
Accession Version	XM_004691619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	eukaryotic translation initiation factor 2-alpha kinase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567132.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2015 this sequence version replaced XM_004691619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCG CCACCCGCCC GGGGTCGCTA GGTCGGGCGC TGTTGCTGCA GTTGCTGCTG 
GGGTTCGCGG CGGGGACTGT GGCCGCGAAG CGCGCATCCG ATCTCCCTGC GTCCACGGCG 
GCGGCGTTCG GCCTGGGAGC TGCGGCCGCC CCCACCTCCG CCGCGAGGGT GCCGGCGGCG 
GGGGTCGTAA CTGCTGCTGA GGTGACGGTG GAAGACGCCG AGGCGCTGCC GGCTGCCACG 
GGCGAACCGG AGCCGCGTGA GCCGGAACTC GACGACGAGG CCGAGCTGCG GCCACGCGGC 
AGATCACTAG TGATTATCAG CACTCTAGAT GGGCGGATTG CGGCCTTGGA TCCTGAGAAC 
CGTGGTAAAA AGCAGTGGGA TTTGGATGTT GGATCCGGGT CCTTGGTTTC CTCCAGCCTT 
AGCAAACCAG AGGTCTTTGG GAATAAGATG ATAATCCCTT CCCTGGATGG AGACCTCTTC 
CAGTGGGACC GAGACCGTGA AAGTATGGAA ACAGTTCCAT TTACAGTTGA ATCGCTTCTT 
GAATCTTCTT ACAAATTTGG AGATGATGTT GTCTTGGTTG GAGGAAAGTC TCTTACCACC 
TATGGACTCA GTGCATATAG TGGAAAGGTG AGGTACATCT GCTCAGCTTT GGGTTGCCGC 
CAGTGGGATA ATGATGAGCT GGAACAGGAA GATACCCTGC TTCTGCAGCG AACCCAGAAA 
ACCGTCAGAG CTGTTGGACC TCGTAGCGGC AATGAGAAGT GGAATTTCAG TGTTGGCCAC 
TTTGAACTTC GGTATATTCC AGACATGGAA ACTAGAGTCG GATTTATTGA AAGTACCTTA 
AAACCCAGTG GGAACAAAGA AGAGTCTAAA ATTATCTCAG ATGTGGAAGA ACAGGAAGCT 
GCCACGATGG ATACAGTGAT AAAGGTCTCA GTTGCTGATT GGAAAGTTAT GGCATTTAAT 
AAGAAAGGAG GACATCTGGA ATGGGAATAC CAGTTTTGTA CTCCAATTGC ATCTGCCTGG 
TTGGTCAAGG ATGGCAAAGT CATTCCCATC AGTCTTTTTG ATGACACGAG TTATACATCT 
AATGATGAGG TTTTAGAAGA TGAAGAAGAC ATTGTAGAAG CTGCCCGAGG AGCCACAGAG 
AACAGCGTTT ACTTGGGAAT GTACAGAGGG CAGCTGTATC TACAGTCATC AGTCAGAATT 
TCAGAAAAGT TTCCTACAAA TCCCAAGGCC TTAGAATCTG TTAATAACGA AAATTCAATT 
ATTCCTTTGC CAACAATCAA ATGGAAACCC TTAATTCATT CTCCTTCCAG AACTCCTGTC 
TTAGTTGGGT CTGATGAATT TGACAAATGT CTTAGTAACG ATAAGTTTTC TCATGAAGAA 
TATAGTAACG GTGCACTTTC AATCCTGCAG TATCCATATG ATAATGGTTA CTATCTCCCA 
TACTACAAGA GGGAAAGGAG CAAACGGAGC ACCCAGATCA CCGTCAGATT TCTCGAAAAC 
CCCAGTTACA ACAAGAACAT CCGCAAAAAG GATCCGGTTC TTCTCTTACA CTGGTGGAAA 
GAAATAGTTG GAACTATTCT TTTTTGTATT GTAGCAACAA CTTTTATTGT ACGCAAACTT 
TTCCACCCTC ATCCTCACAG ACAAAGGAAG GAATCTGAAA CTCAGTGTCA AACTGAAAGT 
AAATATGATT CTGTAAGTGG AGAAGCCAGT GACAGCATCT GTAGTGACAT AAAAAATTCC 
GGATATGTGT CCCGCTATCT AACAGATTTT GAACCAGTTC AATGCATGGG TCGTGGTGGG 
TTTGGAGTTG TCTTTGAAGC TAGAAACAAA GTAGATGACT GCAATTACGC TATCAAGAGA 
ATCCGTCTCC CCAACAGGGA GTTGGCCCGG GAAAAGGTAA TGAGAGAAGT TAAAGCCTTA 
GCTAAGCTTG AACACCCAGG AATTGTTAGA TACTTCAATG CTTGGTTGGA AGCACCACCA 
GAAAAGTGGC AAGAAAAAAT GGATGAAATC TGGCTAAAAG ATGAAAGCAC AGACTGGCCG 
GTCAGCTCTC CTAGTCCAAT GGATGCACCC TCCGTTAAAA TACGCAGAAT GGATCCTTTC 
TCTACAAAGG AACATATTGA AATCATAGCT CCTTCACCAC GAAGAAGCAG GTCTTTCTCT 
GTAGGGATTT CCTGTGGCCA GACAAGCTCA TCCGAGAGCC AGTTCTCTCC GCTGGAATTT 
TCCGATACAG ACCATGGAGA TGTCAGTGAG TCAGTGGGGG GCGCATGTAA TCTCCCTGAC 
AGCTGCCTCG CGGACTGTGA TGTGGAAGAC GGGACCCTTG ACGGCAATGA TGAGGGTCAC 
TCCTTTGAAC TTTGTCCTTC TGAAGCCTCT CCTTTTGTAA GGGAGAGAAC CTCATCTTCT 
ATAGTATTTG AAGATTCTGG CTGTGATAAT GCATCCAGTA AAGAAGAACT CAAAACTAAC 
CGACTGCGTA TTGGCAGCCA TTATGCTAAT AAACTCACTG CTCTCAAGCA TTCCACTAGC 
AAATCTTCTT CCGAACCAAC TTTGTCTATT TCTCCTCCAA GACCAACCAC TTTAAGTTTA 
GATCTCACTA AAAACACGGC AGAGAAACTC CAGCCCAGTT CCCCGAAGGT CTATCTTTAC 
ATTCAGATGC AGCTGTGCAG AAAGGAAAAC CTCAAAGACT GGATGAACAG CCGCTGCACC 
ATAGACGAGC GCGAGAGGAG CGTGTGTCTG CACATCTTCC TGCAGATCGC CGAGGCCGTG 
GAGTTTTTGC ACAGCAAAGG ACTCATGCAT AGGGACCTCA AGCCGTCCAA TATATTCTTT 
ACCATGGACG ACGTGGTCAA GGTTGGGGAC TTTGGATTAG TGACCGCAAT GGACCAAGAC 
GAGGAAGAGC AGCTGGTACT AACCCCAATG CCAGCATATG CCAGACACAC AGGACAAGTG 
GGCACCAAAC TATACATGAG CCCAGAGCAG ATTCATGGAA ACAACTACTC TCATAAAGTG 
GACATCTTTT CTTTAGGCCT CATTCTATTC GAATTGCTGT ACCCATTTGG CACTCAGATG 
GAGAGAGTGA GGATCTTAAC TGATGTAAGA AATCTAAAAT TCCCACCACT GTTTACTCAG 
AAATATCCTC GTGAGTATGC GATGGTTCAA GATATGCTCT CTCCATCACC CACTGAAAGG 
CCTGAAGCTA CTGACATCAT TGAAAATGCT GTGTTTGAGG ACTTGGAACT TCCAGGAAAG 
ACAGTGCTCA GACAAAGATC TCGCTCCTTG AGTTCACCAG GAACAAAACA CACAAGACAG 
CCCAGCATCT CCCATATCCC TTTGCCAAGC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004691676.1
CDS	303..3638
Translation	MERATRPGSLGRALLLQLLLGFAAGTVAAKRASDLPASTAAAFGLGAAAAPTSAARVPAAGVVTAAEVTVEDAEALPAATGEPEPREPELDDEAELRPRGRSLVIISTLDGRIAALDPENRGKKQWDLDVGSGSLVSSSLSKPEVFGNKMIIPSLDGDLFQWDRDRESMETVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSGKVRYICSALGCRQWDNDELEQEDTLLLQRTQKTVRAVGPRSGNEKWNFSVGHFELRYIPDMETRVGFIESTLKPSGNKEESKIISDVEEQEAATMDTVIKVSVADWKVMAFNKKGGHLEWEYQFCTPIASAWLVKDGKVIPISLFDDTSYTSNDEVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRISEKFPTNPKALESVNNENSIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDKFSHEEYSNGALSILQYPYDNGYYLPYYKRERSKRSTQITVRFLENPSYNKNIRKKDPVLLLHWWKEIVGTILFCIVATTFIVRKLFHPHPHRQRKESETQCQTESKYDSVSGEASDSICSDIKNSGYVSRYLTDFEPVQCMGRGGFGVVFEARNKVDDCNYAIKRIRLPNRELAREKVMREVKALAKLEHPGIVRYFNAWLEAPPEKWQEKMDEIWLKDESTDWPVSSPSPMDAPSVKIRRMDPFSTKEHIEIIAPSPRRSRSFSVGISCGQTSSSESQFSPLEFSDTDHGDVSESVGGACNLPDSCLADCDVEDGTLDGNDEGHSFELCPSEASPFVRERTSSSIVFEDSGCDNASSKEELKTNRLRIGSHYANKLTALKHSTSKSSSEPTLSISPPRPTTLSLDLTKNTAEKLQPSSPKVYLYIQMQLCRKENLKDWMNSRCTIDERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQLVLTPMPAYARHTGQVGTKLYMSPEQIHGNNYSHKVDIFSLGLILFELLYPFGTQMERVRILTDVRNLKFPPLFTQKYPREYAMVQDMLSPSPTERPEATDIIENAVFEDLELPGKTVLRQRSRSLSSPGTKHTRQPSISHIPLPSN

Target ORF information:

RefSeq Version	XM_004691619.2
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004691619.2

ORF Insert Sequence:

ATGGAGCGCG CCACCCGCCC GGGGTCGCTA GGTCGGGCGC TGTTGCTGCA GTTGCTGCTG 
GGGTTCGCGG CGGGGACTGT GGCCGCGAAG CGCGCATCCG ATCTCCCTGC GTCCACGGCG 
GCGGCGTTCG GCCTGGGAGC TGCGGCCGCC CCCACCTCCG CCGCGAGGGT GCCGGCGGCG 
GGGGTCGTAA CTGCTGCTGA GGTGACGGTG GAAGACGCCG AGGCGCTGCC GGCTGCCACG 
GGCGAACCGG AGCCGCGTGA GCCGGAACTC GACGACGAGG CCGAGCTGCG GCCACGCGGC 
AGATCACTAG TGATTATCAG CACTCTAGAT GGGCGGATTG CGGCCTTGGA TCCTGAGAAC 
CGTGGTAAAA AGCAGTGGGA TTTGGATGTT GGATCCGGGT CCTTGGTTTC CTCCAGCCTT 
AGCAAACCAG AGGTCTTTGG GAATAAGATG ATAATCCCTT CCCTGGATGG AGACCTCTTC 
CAGTGGGACC GAGACCGTGA AAGTATGGAA ACAGTTCCAT TTACAGTTGA ATCGCTTCTT 
GAATCTTCTT ACAAATTTGG AGATGATGTT GTCTTGGTTG GAGGAAAGTC TCTTACCACC 
TATGGACTCA GTGCATATAG TGGAAAGGTG AGGTACATCT GCTCAGCTTT GGGTTGCCGC 
CAGTGGGATA ATGATGAGCT GGAACAGGAA GATACCCTGC TTCTGCAGCG AACCCAGAAA 
ACCGTCAGAG CTGTTGGACC TCGTAGCGGC AATGAGAAGT GGAATTTCAG TGTTGGCCAC 
TTTGAACTTC GGTATATTCC AGACATGGAA ACTAGAGTCG GATTTATTGA AAGTACCTTA 
AAACCCAGTG GGAACAAAGA AGAGTCTAAA ATTATCTCAG ATGTGGAAGA ACAGGAAGCT 
GCCACGATGG ATACAGTGAT AAAGGTCTCA GTTGCTGATT GGAAAGTTAT GGCATTTAAT 
AAGAAAGGAG GACATCTGGA ATGGGAATAC CAGTTTTGTA CTCCAATTGC ATCTGCCTGG 
TTGGTCAAGG ATGGCAAAGT CATTCCCATC AGTCTTTTTG ATGACACGAG TTATACATCT 
AATGATGAGG TTTTAGAAGA TGAAGAAGAC ATTGTAGAAG CTGCCCGAGG AGCCACAGAG 
AACAGCGTTT ACTTGGGAAT GTACAGAGGG CAGCTGTATC TACAGTCATC AGTCAGAATT 
TCAGAAAAGT TTCCTACAAA TCCCAAGGCC TTAGAATCTG TTAATAACGA AAATTCAATT 
ATTCCTTTGC CAACAATCAA ATGGAAACCC TTAATTCATT CTCCTTCCAG AACTCCTGTC 
TTAGTTGGGT CTGATGAATT TGACAAATGT CTTAGTAACG ATAAGTTTTC TCATGAAGAA 
TATAGTAACG GTGCACTTTC AATCCTGCAG TATCCATATG ATAATGGTTA CTATCTCCCA 
TACTACAAGA GGGAAAGGAG CAAACGGAGC ACCCAGATCA CCGTCAGATT TCTCGAAAAC 
CCCAGTTACA ACAAGAACAT CCGCAAAAAG GATCCGGTTC TTCTCTTACA CTGGTGGAAA 
GAAATAGTTG GAACTATTCT TTTTTGTATT GTAGCAACAA CTTTTATTGT ACGCAAACTT 
TTCCACCCTC ATCCTCACAG ACAAAGGAAG GAATCTGAAA CTCAGTGTCA AACTGAAAGT 
AAATATGATT CTGTAAGTGG AGAAGCCAGT GACAGCATCT GTAGTGACAT AAAAAATTCC 
GGATATGTGT CCCGCTATCT AACAGATTTT GAACCAGTTC AATGCATGGG TCGTGGTGGG 
TTTGGAGTTG TCTTTGAAGC TAGAAACAAA GTAGATGACT GCAATTACGC TATCAAGAGA 
ATCCGTCTCC CCAACAGGGA GTTGGCCCGG GAAAAGGTAA TGAGAGAAGT TAAAGCCTTA 
GCTAAGCTTG AACACCCAGG AATTGTTAGA TACTTCAATG CTTGGTTGGA AGCACCACCA 
GAAAAGTGGC AAGAAAAAAT GGATGAAATC TGGCTAAAAG ATGAAAGCAC AGACTGGCCG 
GTCAGCTCTC CTAGTCCAAT GGATGCACCC TCCGTTAAAA TACGCAGAAT GGATCCTTTC 
TCTACAAAGG AACATATTGA AATCATAGCT CCTTCACCAC GAAGAAGCAG GTCTTTCTCT 
GTAGGGATTT CCTGTGGCCA GACAAGCTCA TCCGAGAGCC AGTTCTCTCC GCTGGAATTT 
TCCGATACAG ACCATGGAGA TGTCAGTGAG TCAGTGGGGG GCGCATGTAA TCTCCCTGAC 
AGCTGCCTCG CGGACTGTGA TGTGGAAGAC GGGACCCTTG ACGGCAATGA TGAGGGTCAC 
TCCTTTGAAC TTTGTCCTTC TGAAGCCTCT CCTTTTGTAA GGGAGAGAAC CTCATCTTCT 
ATAGTATTTG AAGATTCTGG CTGTGATAAT GCATCCAGTA AAGAAGAACT CAAAACTAAC 
CGACTGCGTA TTGGCAGCCA TTATGCTAAT AAACTCACTG CTCTCAAGCA TTCCACTAGC 
AAATCTTCTT CCGAACCAAC TTTGTCTATT TCTCCTCCAA GACCAACCAC TTTAAGTTTA 
GATCTCACTA AAAACACGGC AGAGAAACTC CAGCCCAGTT CCCCGAAGGT CTATCTTTAC 
ATTCAGATGC AGCTGTGCAG AAAGGAAAAC CTCAAAGACT GGATGAACAG CCGCTGCACC 
ATAGACGAGC GCGAGAGGAG CGTGTGTCTG CACATCTTCC TGCAGATCGC CGAGGCCGTG 
GAGTTTTTGC ACAGCAAAGG ACTCATGCAT AGGGACCTCA AGCCGTCCAA TATATTCTTT 
ACCATGGACG ACGTGGTCAA GGTTGGGGAC TTTGGATTAG TGACCGCAAT GGACCAAGAC 
GAGGAAGAGC AGCTGGTACT AACCCCAATG CCAGCATATG CCAGACACAC AGGACAAGTG 
GGCACCAAAC TATACATGAG CCCAGAGCAG ATTCATGGAA ACAACTACTC TCATAAAGTG 
GACATCTTTT CTTTAGGCCT CATTCTATTC GAATTGCTGT ACCCATTTGG CACTCAGATG 
GAGAGAGTGA GGATCTTAAC TGATGTAAGA AATCTAAAAT TCCCACCACT GTTTACTCAG 
AAATATCCTC GTGAGTATGC GATGGTTCAA GATATGCTCT CTCCATCACC CACTGAAAGG 
CCTGAAGCTA CTGACATCAT TGAAAATGCT GTGTTTGAGG ACTTGGAACT TCCAGGAAAG 
ACAGTGCTCA GACAAAGATC TCGCTCCTTG AGTTCACCAG GAACAAAACA CACAAGACAG 
CCCAGCATCT CCCATATCCC TTTGCCAAGC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK3 ( XM_012731596.1 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_012587050.1 Condylura cristata eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCo243401
Clone ID Related Accession (Same CDS sequence)	XM_012731596.1
Accession Version	XM_012731596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	eukaryotic translation initiation factor 2-alpha kinase 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567132.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCG CCACCCGCCC GGGGTCGCTA GGTCGGGCGC TGTTGCTGCA GTTGCTGCTG 
GGGTTCGCGG CGGGGACTGT GGCCGCGAAG CGCGCATCCG ATCTCCCTGC GTCCACGGCG 
GCGGCGTTCG GCCTGGGAGC TGCGGCCGCC CCCACCTCCG CCGCGAGGGT GCCGGCGGCG 
GGGGTCGTAA CTGCTGCTGA GGTGACGGTG GAAGACGCCG AGGCGCTGCC GGCTGCCACG 
GGCGAACCGG AGCCGCGTGA GCCGGAACTC GACGACGAGG CCGAGCTGCG GCCACGCGGC 
AGATCACTAG TGATTATCAG CACTCTAGAT GGGCGGATTG CGGCCTTGGA TCCTGAGAAC 
CGTGGTAAAA AGCAGTGGGA TTTGGATGTT GGATCCGGGT CCTTGGTTTC CTCCAGCCTT 
AGCAAACCAG AGGTCTTTGG GAATAAGATG ATAATCCCTT CCCTGGATGG AGACCTCTTC 
CAGTGGGACC GAGACCGTGA AAGTATGGAA ACAGTTCCAT TTACAGTTGA ATCGCTTCTT 
GAATCTTCTT ACAAATTTGG AGATGATGTT GTCTTGGTTG GAGGAAAGTC TCTTACCACC 
TATGGACTCA GTGCATATAG TGGAAAGGTG AGGTACATCT GCTCAGCTTT GGGTTGCCGC 
CAGTGGGATA ATGATGAGCT GGAACAGGAA GATACCCTGC TTCTGCAGCG AACCCAGAAA 
ACCGTCAGAG CTGTTGGACC TCGTAGCGGC AATGAGAAGT GGAATTTCAG TGTTGGCCAC 
TTTGAACTTC GGTATATTCC AGACATGGAA ACTAGAGTCG GATTTATTGA AAGTACCTTA 
AAACCCAGTG GGAACAAAGA AGAGTCTAAA ATTATCTCAG ATGTGGAAGA ACAGGAAGCT 
GCCACGATGG ATACAGTGAT AAAGGTCTCA GTTGCTGATT GGAAAGTTAT GGCATTTAAT 
AAGAAAGGAG GACATCTGGA ATGGGAATAC CAGTTTTGTA CTCCAATTGC ATCTGCCTGG 
TTGGTCAAGG ATGGCAAAGT CATTCCCATC AGTCTTTTTG ATGACACGAG TTATACATCT 
AATGATGAGG TTTTAGAAGA TGAAGAAGAC ATTGTAGAAG CTGCCCGAGG AGCCACAGAG 
AACAGCGTTT ACTTGGGAAT GTACAGAGGG CAGCTGTATC TACAGTCATC AGTCAGAATT 
TCAGAAAAGT TTCCTACAAA TCCCAAGGCC TTAGAATCTG TTAATAACGA AAATTCAATT 
ATTCCTTTGC CAACAATCAA ATGGAAACCC TTAATTCATT CTCCTTCCAG AACTCCTGTC 
TTAGTTGGGT CTGATGAATT TGACAAATGT CTTAGTAACG ATAAGTTTTC TCATGAAGAA 
TATAGTAACG GTGCACTTTC AATCCTGCAG TATCCATATG ATAATGGTTA CTATCTCCCA 
TACTACAAGA GGGAAAGGAG CAAACGGAGC ACCCAGATCA CCGTCAGATT TCTCGAAAAC 
CCCAGTTACA ACAAGAACAT CCGCAAAAAG GATCCGGTTC TTCTCTTACA CTGGTGGAAA 
GAAATAGTTG GAACTATTCT TTTTTGTATT GTAGCAACAA CTTTTATTGT ACGCAAACTT 
TTCCACCCTC ATCCTCACAG ACAAAGGAAG GAATCTGAAA CTCAGTGTCA AACTGAAAGT 
AAATATGATT CTGTAAGTGG AGAAGCCAGT GACAGCATCT GTAGTGACAT AAAAAATTCC 
GGATATGTGT CCCGGGAGTT GGCCCGGGAA AAGGTAATGA GAGAAGTTAA AGCCTTAGCT 
AAGCTTGAAC ACCCAGGAAT TGTTAGATAC TTCAATGCTT GGTTGGAAGC ACCACCAGAA 
AAGTGGCAAG AAAAAATGGA TGAAATCTGG CTAAAAGATG AAAGCACAGA CTGGCCGGTC 
AGCTCTCCTA GTCCAATGGA TGCACCCTCC GTTAAAATAC GCAGAATGGA TCCTTTCTCT 
ACAAAGGAAC ATATTGAAAT CATAGCTCCT TCACCACGAA GAAGCAGGTC TTTCTCTGTA 
GGGATTTCCT GTGGCCAGAC AAGCTCATCC GAGAGCCAGT TCTCTCCGCT GGAATTTTCC 
GATACAGACC ATGGAGATGT CAGTGAGTCA GTGGGGGGCG CATGTAATCT CCCTGACAGC 
TGCCTCGCGG ACTGTGATGT GGAAGACGGG ACCCTTGACG GCAATGATGA GGGTCACTCC 
TTTGAACTTT GTCCTTCTGA AGCCTCTCCT TTTGTAAGGG AGAGAACCTC ATCTTCTATA 
GTATTTGAAG ATTCTGGCTG TGATAATGCA TCCAGTAAAG AAGAACTCAA AACTAACCGA 
CTGCGTATTG GCAGCCATTA TGCTAATAAA CTCACTGCTC TCAAGCATTC CACTAGCAAA 
TCTTCTTCCG AACCAACTTT GTCTATTTCT CCTCCAAGAC CAACCACTTT AAGTTTAGAT 
CTCACTAAAA ACACGGCAGA GAAACTCCAG CCCAGTTCCC CGAAGGTCTA TCTTTACATT 
CAGATGCAGC TGTGCAGAAA GGAAAACCTC AAAGACTGGA TGAACAGCCG CTGCACCATA 
GACGAGCGCG AGAGGAGCGT GTGTCTGCAC ATCTTCCTGC AGATCGCCGA GGCCGTGGAG 
TTTTTGCACA GCAAAGGACT CATGCATAGG GACCTCAAGC CGTCCAATAT ATTCTTTACC 
ATGGACGACG TGGTCAAGGT TGGGGACTTT GGATTAGTGA CCGCAATGGA CCAAGACGAG 
GAAGAGCAGC TGGTACTAAC CCCAATGCCA GCATATGCCA GACACACAGG ACAAGTGGGC 
ACCAAACTAT ACATGAGCCC AGAGCAGATT CATGGAAACA ACTACTCTCA TAAAGTGGAC 
ATCTTTTCTT TAGGCCTCAT TCTATTCGAA TTGCTGTACC CATTTGGCAC TCAGATGGAG 
AGAGTGAGGA TCTTAACTGA TGTAAGAAAT CTAAAATTCC CACCACTGTT TACTCAGAAA 
TATCCTCGTG AGTATGCGAT GGTTCAAGAT ATGCTCTCTC CATCACCCAC TGAAAGGCCT 
GAAGCTACTG ACATCATTGA AAATGCTGTG TTTGAGGACT TGGAACTTCC AGGAAAGACA 
GTGCTCAGAC AAAGATCTCG CTCCTTGAGT TCACCAGGAA CAAAACACAC AAGACAGCCC 
AGCATCTCCC ATATCCCTTT GCCAAGCAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012587050.1
CDS	134..3346
Translation	MERATRPGSLGRALLLQLLLGFAAGTVAAKRASDLPASTAAAFGLGAAAAPTSAARVPAAGVVTAAEVTVEDAEALPAATGEPEPREPELDDEAELRPRGRSLVIISTLDGRIAALDPENRGKKQWDLDVGSGSLVSSSLSKPEVFGNKMIIPSLDGDLFQWDRDRESMETVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSGKVRYICSALGCRQWDNDELEQEDTLLLQRTQKTVRAVGPRSGNEKWNFSVGHFELRYIPDMETRVGFIESTLKPSGNKEESKIISDVEEQEAATMDTVIKVSVADWKVMAFNKKGGHLEWEYQFCTPIASAWLVKDGKVIPISLFDDTSYTSNDEVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRISEKFPTNPKALESVNNENSIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDKFSHEEYSNGALSILQYPYDNGYYLPYYKRERSKRSTQITVRFLENPSYNKNIRKKDPVLLLHWWKEIVGTILFCIVATTFIVRKLFHPHPHRQRKESETQCQTESKYDSVSGEASDSICSDIKNSGYVSRELAREKVMREVKALAKLEHPGIVRYFNAWLEAPPEKWQEKMDEIWLKDESTDWPVSSPSPMDAPSVKIRRMDPFSTKEHIEIIAPSPRRSRSFSVGISCGQTSSSESQFSPLEFSDTDHGDVSESVGGACNLPDSCLADCDVEDGTLDGNDEGHSFELCPSEASPFVRERTSSSIVFEDSGCDNASSKEELKTNRLRIGSHYANKLTALKHSTSKSSSEPTLSISPPRPTTLSLDLTKNTAEKLQPSSPKVYLYIQMQLCRKENLKDWMNSRCTIDERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQLVLTPMPAYARHTGQVGTKLYMSPEQIHGNNYSHKVDIFSLGLILFELLYPFGTQMERVRILTDVRNLKFPPLFTQKYPREYAMVQDMLSPSPTERPEATDIIENAVFEDLELPGKTVLRQRSRSLSSPGTKHTRQPSISHIPLPSN

Target ORF information:

RefSeq Version	XM_012731596.1
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012731596.1

ORF Insert Sequence:

ATGGAGCGCG CCACCCGCCC GGGGTCGCTA GGTCGGGCGC TGTTGCTGCA GTTGCTGCTG 
GGGTTCGCGG CGGGGACTGT GGCCGCGAAG CGCGCATCCG ATCTCCCTGC GTCCACGGCG 
GCGGCGTTCG GCCTGGGAGC TGCGGCCGCC CCCACCTCCG CCGCGAGGGT GCCGGCGGCG 
GGGGTCGTAA CTGCTGCTGA GGTGACGGTG GAAGACGCCG AGGCGCTGCC GGCTGCCACG 
GGCGAACCGG AGCCGCGTGA GCCGGAACTC GACGACGAGG CCGAGCTGCG GCCACGCGGC 
AGATCACTAG TGATTATCAG CACTCTAGAT GGGCGGATTG CGGCCTTGGA TCCTGAGAAC 
CGTGGTAAAA AGCAGTGGGA TTTGGATGTT GGATCCGGGT CCTTGGTTTC CTCCAGCCTT 
AGCAAACCAG AGGTCTTTGG GAATAAGATG ATAATCCCTT CCCTGGATGG AGACCTCTTC 
CAGTGGGACC GAGACCGTGA AAGTATGGAA ACAGTTCCAT TTACAGTTGA ATCGCTTCTT 
GAATCTTCTT ACAAATTTGG AGATGATGTT GTCTTGGTTG GAGGAAAGTC TCTTACCACC 
TATGGACTCA GTGCATATAG TGGAAAGGTG AGGTACATCT GCTCAGCTTT GGGTTGCCGC 
CAGTGGGATA ATGATGAGCT GGAACAGGAA GATACCCTGC TTCTGCAGCG AACCCAGAAA 
ACCGTCAGAG CTGTTGGACC TCGTAGCGGC AATGAGAAGT GGAATTTCAG TGTTGGCCAC 
TTTGAACTTC GGTATATTCC AGACATGGAA ACTAGAGTCG GATTTATTGA AAGTACCTTA 
AAACCCAGTG GGAACAAAGA AGAGTCTAAA ATTATCTCAG ATGTGGAAGA ACAGGAAGCT 
GCCACGATGG ATACAGTGAT AAAGGTCTCA GTTGCTGATT GGAAAGTTAT GGCATTTAAT 
AAGAAAGGAG GACATCTGGA ATGGGAATAC CAGTTTTGTA CTCCAATTGC ATCTGCCTGG 
TTGGTCAAGG ATGGCAAAGT CATTCCCATC AGTCTTTTTG ATGACACGAG TTATACATCT 
AATGATGAGG TTTTAGAAGA TGAAGAAGAC ATTGTAGAAG CTGCCCGAGG AGCCACAGAG 
AACAGCGTTT ACTTGGGAAT GTACAGAGGG CAGCTGTATC TACAGTCATC AGTCAGAATT 
TCAGAAAAGT TTCCTACAAA TCCCAAGGCC TTAGAATCTG TTAATAACGA AAATTCAATT 
ATTCCTTTGC CAACAATCAA ATGGAAACCC TTAATTCATT CTCCTTCCAG AACTCCTGTC 
TTAGTTGGGT CTGATGAATT TGACAAATGT CTTAGTAACG ATAAGTTTTC TCATGAAGAA 
TATAGTAACG GTGCACTTTC AATCCTGCAG TATCCATATG ATAATGGTTA CTATCTCCCA 
TACTACAAGA GGGAAAGGAG CAAACGGAGC ACCCAGATCA CCGTCAGATT TCTCGAAAAC 
CCCAGTTACA ACAAGAACAT CCGCAAAAAG GATCCGGTTC TTCTCTTACA CTGGTGGAAA 
GAAATAGTTG GAACTATTCT TTTTTGTATT GTAGCAACAA CTTTTATTGT ACGCAAACTT 
TTCCACCCTC ATCCTCACAG ACAAAGGAAG GAATCTGAAA CTCAGTGTCA AACTGAAAGT 
AAATATGATT CTGTAAGTGG AGAAGCCAGT GACAGCATCT GTAGTGACAT AAAAAATTCC 
GGATATGTGT CCCGGGAGTT GGCCCGGGAA AAGGTAATGA GAGAAGTTAA AGCCTTAGCT 
AAGCTTGAAC ACCCAGGAAT TGTTAGATAC TTCAATGCTT GGTTGGAAGC ACCACCAGAA 
AAGTGGCAAG AAAAAATGGA TGAAATCTGG CTAAAAGATG AAAGCACAGA CTGGCCGGTC 
AGCTCTCCTA GTCCAATGGA TGCACCCTCC GTTAAAATAC GCAGAATGGA TCCTTTCTCT 
ACAAAGGAAC ATATTGAAAT CATAGCTCCT TCACCACGAA GAAGCAGGTC TTTCTCTGTA 
GGGATTTCCT GTGGCCAGAC AAGCTCATCC GAGAGCCAGT TCTCTCCGCT GGAATTTTCC 
GATACAGACC ATGGAGATGT CAGTGAGTCA GTGGGGGGCG CATGTAATCT CCCTGACAGC 
TGCCTCGCGG ACTGTGATGT GGAAGACGGG ACCCTTGACG GCAATGATGA GGGTCACTCC 
TTTGAACTTT GTCCTTCTGA AGCCTCTCCT TTTGTAAGGG AGAGAACCTC ATCTTCTATA 
GTATTTGAAG ATTCTGGCTG TGATAATGCA TCCAGTAAAG AAGAACTCAA AACTAACCGA 
CTGCGTATTG GCAGCCATTA TGCTAATAAA CTCACTGCTC TCAAGCATTC CACTAGCAAA 
TCTTCTTCCG AACCAACTTT GTCTATTTCT CCTCCAAGAC CAACCACTTT AAGTTTAGAT 
CTCACTAAAA ACACGGCAGA GAAACTCCAG CCCAGTTCCC CGAAGGTCTA TCTTTACATT 
CAGATGCAGC TGTGCAGAAA GGAAAACCTC AAAGACTGGA TGAACAGCCG CTGCACCATA 
GACGAGCGCG AGAGGAGCGT GTGTCTGCAC ATCTTCCTGC AGATCGCCGA GGCCGTGGAG 
TTTTTGCACA GCAAAGGACT CATGCATAGG GACCTCAAGC CGTCCAATAT ATTCTTTACC 
ATGGACGACG TGGTCAAGGT TGGGGACTTT GGATTAGTGA CCGCAATGGA CCAAGACGAG 
GAAGAGCAGC TGGTACTAAC CCCAATGCCA GCATATGCCA GACACACAGG ACAAGTGGGC 
ACCAAACTAT ACATGAGCCC AGAGCAGATT CATGGAAACA ACTACTCTCA TAAAGTGGAC 
ATCTTTTCTT TAGGCCTCAT TCTATTCGAA TTGCTGTACC CATTTGGCAC TCAGATGGAG 
AGAGTGAGGA TCTTAACTGA TGTAAGAAAT CTAAAATTCC CACCACTGTT TACTCAGAAA 
TATCCTCGTG AGTATGCGAT GGTTCAAGAT ATGCTCTCTC CATCACCCAC TGAAAGGCCT 
GAAGCTACTG ACATCATTGA AAATGCTGTG TTTGAGGACT TGGAACTTCC AGGAAAGACA 
GTGCTCAGAC AAAGATCTCG CTCCTTGAGT TCACCAGGAA CAAAACACAC AAGACAGCCC 
AGCATCTCCC ATATCCCTTT GCCAAGCAAT TAG