NEFL cDNA ORF clone, Condylura cristata(star-nosed mole)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFL
Entrez Gene ID	101624266
Full Name	neurofilament light
Gene Type	protein-coding
Organism	Condylura cristata(star-nosed mole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004685388.2	XP_004685445.1	neurofilament light polypeptide

Pathways from BioSystems
Gene Ontology

The following NEFL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo236203	XM_004685388.2 Latest version!	Condylura cristata neurofilament, light polypeptide (NEFL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFL ( XM_004685388.2 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_004685445.1 Condylura cristata neurofilament, light polypeptide (NEFL), mRNA.

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CloneID	OCo236203
Clone ID Related Accession (Same CDS sequence)	XM_004685388.2
Accession Version	XM_004685388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	neurofilament light polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567119.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2015 this sequence version replaced XM_004685388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCT TCAGCTACGA GCCGTACTAC TCGACCTCCT ACAAGCGGCG CTACGTGGAG 
TCGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCCGCTTAC 
TCCAGCTACT CGGCCCCCGT CTCCTCCTCG CTGTCCGTGC GCCGCAGCTA CTCGTCCAGC 
TCCGGCTCCT TGATGCCCAG TCTGGAAAAT CTCGACCTGA GCCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACGCAGGAG AAGGCGCAGC TGCAAGACCT CAACGACCGC 
TTCGCCTGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT TCTGGAGGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCGTCCC GCTTCCGGGC GCTGTACGAG 
CAGGAGATCC GCGACCTACG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTC 
CAGGGCGAGC GCGAGGGGCT GGAGGAGACG CTGCGCAGCC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGCTTGATGG AAGCGCGCAA AGGAGCCGAC 
GAGGCCGCTC TGGCTCGCGC CGAGCTCGAA AAGCGCATCG ACAGCCTGAT GGATGAAATC 
GCCTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCGCA GATTCAGTAC 
GCGCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC GGCGCTCAAG 
GACATCCGCG CCCAGTACGA GAAGCTGGCG GCCAAGAACA TGCAGAACGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACCGT GCTGACCGAA AGCGCCGCCA AGAACACAGA CGCCGTGCGG 
GCCGCCAAGG ACGAGGTGTC GGAGAGCCGC CGCCTGCTCA AGGCCAAGAC GCTGGAGATT 
GAAGCGTGCA GGGGCATGAA CGAGGCGCTG GAGAAGCAGC TGCAGGAGCT GGAGGACAAA 
CAGAACGCTG ACATTAGCGC TATGCAGGAC ACAATCAACA AATTAGAAAA TGAACTAAGG 
ACCACAAAGA GTGAAATGGC ACGATACCTA AAAGAATACC AAGACCTGCT CAATGTGAAG 
ATGGCTTTGG ATATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGCGA GGAGACCCGG 
CTCAGCTTCA CCAGCGTGGG AAGCATAACC AGCGGCTACT CTCAGAGCTC CCAGATGTTC 
GGCAGATCTG CATATGGAGG TTTACAGACC AGCTCCTACT TGATGTCCGC CCGCTCCTTC 
CCCTCCTATT ACACCAGCCA TGTTCAGGAG GAGCAGATAG AAGTGGAGGA GACCATTGAG 
GCTGCAAAGG CTGAGGAAGC CAAGGACGAA CCCCCCTCTG AAGGAGAAGC TGAAGAAGAG 
GAGAAGGAGA AGGAAGAGGC TGAGGAGGAG GAGGGGGCCG AGGAGGAAGA AGCAGCCAAG 
GAAGAGTCAG AAGATGCAAA GGAGGAAGAA GAAGGAGGTG AAGGTGAAGA AGAAGAAGAA 
ACCAAAGAAG CTGAAGAAGA TGAGAAAAAA GATGAAGGTG CAGGGGAGGA GCAAGCAACA 
AAAAAGAAAG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004685445.1
CDS	316..1950
Translation	MSSFSYEPYYSTSYKRRYVESPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFACFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRSLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQMFGRSAYGGLQTSSYLMSARSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKEKEEAEEEEGAEEEEAAKEESEDAKEEEEGGEGEEEEETKEAEEDEKKDEGAGEEQATKKKD

Target ORF information:

RefSeq Version	XM_004685388.2
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata neurofilament, light polypeptide (NEFL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004685388.2

ORF Insert Sequence:

ATGAGTTCCT TCAGCTACGA GCCGTACTAC TCGACCTCCT ACAAGCGGCG CTACGTGGAG 
TCGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCCGCTTAC 
TCCAGCTACT CGGCCCCCGT CTCCTCCTCG CTGTCCGTGC GCCGCAGCTA CTCGTCCAGC 
TCCGGCTCCT TGATGCCCAG TCTGGAAAAT CTCGACCTGA GCCAGGTAGC CGCCATCAGC 
AACGACCTCA AGTCGATCCG CACGCAGGAG AAGGCGCAGC TGCAAGACCT CAACGACCGC 
TTCGCCTGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT TCTGGAGGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCGTCCC GCTTCCGGGC GCTGTACGAG 
CAGGAGATCC GCGACCTACG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCGCTC 
CAGGGCGAGC GCGAGGGGCT GGAGGAGACG CTGCGCAGCC TGCAGGCGCG CTACGAAGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGCTTGATGG AAGCGCGCAA AGGAGCCGAC 
GAGGCCGCTC TGGCTCGCGC CGAGCTCGAA AAGCGCATCG ACAGCCTGAT GGATGAAATC 
GCCTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCGCA GATTCAGTAC 
GCGCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC GGCGCTCAAG 
GACATCCGCG CCCAGTACGA GAAGCTGGCG GCCAAGAACA TGCAGAACGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACCGT GCTGACCGAA AGCGCCGCCA AGAACACAGA CGCCGTGCGG 
GCCGCCAAGG ACGAGGTGTC GGAGAGCCGC CGCCTGCTCA AGGCCAAGAC GCTGGAGATT 
GAAGCGTGCA GGGGCATGAA CGAGGCGCTG GAGAAGCAGC TGCAGGAGCT GGAGGACAAA 
CAGAACGCTG ACATTAGCGC TATGCAGGAC ACAATCAACA AATTAGAAAA TGAACTAAGG 
ACCACAAAGA GTGAAATGGC ACGATACCTA AAAGAATACC AAGACCTGCT CAATGTGAAG 
ATGGCTTTGG ATATTGAGAT TGCAGCTTAC AGGAAACTCT TGGAAGGCGA GGAGACCCGG 
CTCAGCTTCA CCAGCGTGGG AAGCATAACC AGCGGCTACT CTCAGAGCTC CCAGATGTTC 
GGCAGATCTG CATATGGAGG TTTACAGACC AGCTCCTACT TGATGTCCGC CCGCTCCTTC 
CCCTCCTATT ACACCAGCCA TGTTCAGGAG GAGCAGATAG AAGTGGAGGA GACCATTGAG 
GCTGCAAAGG CTGAGGAAGC CAAGGACGAA CCCCCCTCTG AAGGAGAAGC TGAAGAAGAG 
GAGAAGGAGA AGGAAGAGGC TGAGGAGGAG GAGGGGGCCG AGGAGGAAGA AGCAGCCAAG 
GAAGAGTCAG AAGATGCAAA GGAGGAAGAA GAAGGAGGTG AAGGTGAAGA AGAAGAAGAA 
ACCAAAGAAG CTGAAGAAGA TGAGAAAAAA GATGAAGGTG CAGGGGAGGA GCAAGCAACA 
AAAAAGAAAG ATTGA