OAZ1 cDNA ORF clone, Condylura cristata(star-nosed mole)

Gene
Clones
gRNAs

Summary

Gene Symbol	OAZ1
Entrez Gene ID	101621593
Full Name	ornithine decarboxylase antizyme 1
Gene Type	protein-coding
Organism	Condylura cristata(star-nosed mole)
Summary	The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamine in cells. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis pathway; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as by inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs, and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization (PMID:16120325). [provided by RefSeq, Jul 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289835.1	NP_001276764.1	ornithine decarboxylase antizyme 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Ribosomal frameshifting in decoding antizyme mRNAs from yeast and protists to humans: close to 300 cases reveal remarkable diversity despite underlying conservation.
Ivanov IP, Atkins JF
Nucleic acids research35(6)1842-58(2007)

Mitochondrial localization of antizyme is determined by context-dependent alternative utilization of two AUG initiation codons.
Gandre S, Bercovich Z, Kahana C
Mitochondrion2(4)245-56(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following OAZ1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OAZ1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo90247	NM_001289835.1 Latest version!	Condylura cristata ornithine decarboxylase antizyme 1 (OAZ1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OAZ1 ( NM_001289835.1 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> NP_001276764.1 Condylura cristata ornithine decarboxylase antizyme 1 (OAZ1), mRNA.

Price & Availability↑

CloneID	OCo90247
Clone ID Related Accession (Same CDS sequence)	NM_001289835.1
Accession Version	NM_001289835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Condylura cristata(star-nosed mole)
Product	ornithine decarboxylase antizyme 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJFV01058709.1. On Jan 31, 2014 this sequence version replaced XM_004689377.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02183198 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ribosomal slippage :: inferred from conservation ##RefSeq-Attributes-END##

ATGGTGAAAT CCTCCCTGCA GCGGATCCTC AACAGCCACT GCTTCGCCAG AGAGAAGGAG 
GGGGAGAAAC CCAGCGCTAC CGTCCACGCC AGCTACACCA TGCCGCTTCT CAGCTTGCAT 
AGCAGCGGTG GCCGCAGCAG CGAGAGTTCC AGGGTCTCCC TCAACTGCTG TAGTAACCTG 
GGTCCGGGGC CTCGGTGGTG CTCCGATGTC CCTCACCCAC CCCTGAAGAT CCCAGGTGGG 
CGAGGGAATA GTCAGAGGGA TCACAATCTT TCAGCTAATT TATTTTACTC AGATAATCGG 
CTGAATGTAA CAGAGGAACT AACGTCTAAT AACAAGACAA GGATTCTCAA TGTCCAGTCC 
AGGCTCACAG ACACCAAACA CATTCACTGG AGAGCGGTGC TGAGCGGCGG CTGCCTCTAC 
ATCGAGATCC CCGGCGGTGC CCTGCCCGAG GGCAGCAAAG ACAGCTTTGC AGTTCTACTC 
GAGTTTGCCG AGGAGCAGCT CCGTGCTGAC CACGTCTTCA TTTGCTTCCA CAAAAATCGT 
GACGACAGAG CTGCCTTGCT CCGCACCTTC AGTTTTTTGG GCTTTGAGAT TGTGAGACCA 
GGGCATCCCC TTGTCCCCAA GAGACCCGAC GCTTGCTTCA TGGCCTACAC ATTCGAGAGA 
GAGTCTTCGG GCGAGGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276764.1
CDS	join(74..277,279..758)
Translation	MVKSSLQRILNSHCFAREKEGEKPSATVHASYTMPLLSLHSSGGRSSESSRVSLNCCSNLGPGPRWCSDVPHPPLKIPGGRGNSQRDHNLSANLFYSDNRLNVTEELTSNNKTRILNVQSRLTDTKHIHWRAVLSGGCLYIEIPGGALPEGSKDSFAVLLEFAEEQLRADHVFICFHKNRDDRAALLRTFSFLGFEIVRPGHPLVPKRPDACFMAYTFERESSGEEE

Target ORF information:

RefSeq Version	NM_001289835.1
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata ornithine decarboxylase antizyme 1 (OAZ1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289835.1

ORF Insert Sequence:

ATGGTGAAAT CCTCCCTGCA GCGGATCCTC AACAGCCACT GCTTCGCCAG AGAGAAGGAG 
GGGGAGAAAC CCAGCGCTAC CGTCCACGCC AGCTACACCA TGCCGCTTCT CAGCTTGCAT 
AGCAGCGGTG GCCGCAGCAG CGAGAGTTCC AGGGTCTCCC TCAACTGCTG TAGTAACCTG 
GGTCCGGGGC CTCGGTGGTG CTCCGATGTC CCTCACCCAC CCCTGAAGAT CCCAGGTGGG 
CGAGGGAATA GTCAGAGGGA TCACAATCTT TCAGCTAATT TATTTTACTC AGATAATCGG 
CTGAATGTAA CAGAGGAACT AACGTCTAAT AACAAGACAA GGATTCTCAA TGTCCAGTCC 
AGGCTCACAG ACACCAAACA CATTCACTGG AGAGCGGTGC TGAGCGGCGG CTGCCTCTAC 
ATCGAGATCC CCGGCGGTGC CCTGCCCGAG GGCAGCAAAG ACAGCTTTGC AGTTCTACTC 
GAGTTTGCCG AGGAGCAGCT CCGTGCTGAC CACGTCTTCA TTTGCTTCCA CAAAAATCGT 
GACGACAGAG CTGCCTTGCT CCGCACCTTC AGTTTTTTGG GCTTTGAGAT TGTGAGACCA 
GGGCATCCCC TTGTCCCCAA GAGACCCGAC GCTTGCTTCA TGGCCTACAC ATTCGAGAGA 
GAGTCTTCGG GCGAGGAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ribosomal frameshifting in decoding antizyme mRNAs from yeast and protists to humans: close to 300 cases reveal remarkable diversity despite underlying conservation.
Nucleic acids research35(6)1842-58(2007)
Ivanov IP,Atkins JF

Mitochondrial localization of antizyme is determined by context-dependent alternative utilization of two AUG initiation codons.
Mitochondrion2(4)245-56(2003 Mar)
Gandre S,Bercovich Z,Kahana C