VCL cDNA ORF clone, Condylura cristata(star-nosed mole)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	101620070
Full Name	vinculin
Gene Type	protein-coding
Organism	Condylura cristata(star-nosed mole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004680759.2	XP_004680816.1	vinculin isoform X2
XM_004680760.2	XP_004680817.1	vinculin isoform X1

Pathways from BioSystems
Gene Ontology

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo230953	XM_004680760.2 Latest version!	Condylura cristata vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCo230954	XM_004680759.2 Latest version!	Condylura cristata vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( XM_004680760.2 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_004680817.1 Condylura cristata vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCo230953
Clone ID Related Accession (Same CDS sequence)	XM_004680760.2
Accession Version	XM_004680760.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567112.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2015 this sequence version replaced XM_004680760.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACTCG CACCATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGT GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCAGCC GTGAGCAATC TTGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCGAGGG GTATCCTCTC TGGCACATCA 
GACCTACTTC TCACCTTCGA TGAGGCTGAG GTTCGGAAAA TTATTCGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACCAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAACTGACCC ACCAGGAGCA CCGAGTCATG TTGGTAAACT CAATGAATAC TGTGAAAGAG 
TTATTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATAGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC ATGGGCCAGT 
AAAGATACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCTAAATT GAACCAAGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCACT CCAGGGGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CCTCCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTGCGAGCC 
AGAGGACAAG GAGCCTCACC CATGGCCATG CAGAAAGCCC AGCAGGTATC TCAGGGTCTG 
GATATGCTCA CAGCAAAAGT GGAAAATGCT GCCCGGAAAC TGGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCTCAGAATT GGCTTGCAGA CCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ACCCTAAAGA GAGAGATGAC ATTCTACGTT CTCTTGGAGA AATATCTGCT 
TTGACTAATA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAAGTGGC CACAGCCCTG CAGAACATGC AGACTAAAAC CAATCGGGCT 
GTGGCCAATA GCAGACCCAC CAAAGCAGCA GTCCACCTTG AAGGCAAGAT TGAACAAGCA 
CAGCGATGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGACCTTA TCGTCAAGAT 
CTTCTTGCAA AGTGTGACAG AGTGGATCAG CTGACAGCAC AGCTGGCTGA TCTGGCTGCC 
CGAGGGGAAG GAGAGAGCCC ACAGGCACGA GCACTTGCCT CTCAGCTCCA AGACTCTTTG 
AAGGATCTGA AAACACGGAT GCAGGAAGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGTTAGCA GTGGCAGCCA CAGCACCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCAGCTGTT GGAACTGCTA ATAAGTCAAC AGTGGAAGGC 
ATCCAGGCCT CGGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCCCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC AATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGATAAGTG TAAAGTGGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTG GCTGGGGCAA CCAGCATTGC TCGCCGGGCC 
AACCGAATCC TGTTAGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCTTC TGATGAATTG AGTAAAACCA TTTCTCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAC CCTGGTCTGC AAAAGAGCTT CCTGGACTCG 
GGATATCGGA TCCTCGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAACCT 
GACTTCCCTC CTCCTCCACC AGACATTGAT CAGCTCCGTT TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CCCTGCCCGA GGGTGAGGTC CCTCCACCCA GACCTCCACC ACCAGAAGAG 
AAAGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGTATGGC TCTACTGATG GCTGAGATGT CTCGACTGGT TAGAGGAGGC 
AGTGGTACCA AGAGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA AGTTGCAAAG CAGTGCACGG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG GATCCCAACC ATAAGCACCC AGCTCAAAAT TCTGTCCACT 
GTAAAGGCCA CCATGCTGGG TCGGACCAAC ATCAGCGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TCCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGTGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCAT GGTACCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004680817.1
CDS	85..3285
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNATPGDAGEQAIRQILDEAGKVGELCAGKERREILGTSKMLGQMTDQVADLRARGQGASPMAMQKAQQVSQGLDMLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTNKLADLRRQGKGDSPEARALAKQVATALQNMQTKTNRAVANSRPTKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKTRMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDIDQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_004680760.2
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004680760.2

ORF Insert Sequence:

ATGCCGGTGT TTCATACTCG CACCATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGT GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCAGCC GTGAGCAATC TTGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCGAGGG GTATCCTCTC TGGCACATCA 
GACCTACTTC TCACCTTCGA TGAGGCTGAG GTTCGGAAAA TTATTCGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACCAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAACTGACCC ACCAGGAGCA CCGAGTCATG TTGGTAAACT CAATGAATAC TGTGAAAGAG 
TTATTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATAGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC ATGGGCCAGT 
AAAGATACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCTAAATT GAACCAAGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCACT CCAGGGGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CCTCCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTGCGAGCC 
AGAGGACAAG GAGCCTCACC CATGGCCATG CAGAAAGCCC AGCAGGTATC TCAGGGTCTG 
GATATGCTCA CAGCAAAAGT GGAAAATGCT GCCCGGAAAC TGGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCTCAGAATT GGCTTGCAGA CCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ACCCTAAAGA GAGAGATGAC ATTCTACGTT CTCTTGGAGA AATATCTGCT 
TTGACTAATA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAAGTGGC CACAGCCCTG CAGAACATGC AGACTAAAAC CAATCGGGCT 
GTGGCCAATA GCAGACCCAC CAAAGCAGCA GTCCACCTTG AAGGCAAGAT TGAACAAGCA 
CAGCGATGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGACCTTA TCGTCAAGAT 
CTTCTTGCAA AGTGTGACAG AGTGGATCAG CTGACAGCAC AGCTGGCTGA TCTGGCTGCC 
CGAGGGGAAG GAGAGAGCCC ACAGGCACGA GCACTTGCCT CTCAGCTCCA AGACTCTTTG 
AAGGATCTGA AAACACGGAT GCAGGAAGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGTTAGCA GTGGCAGCCA CAGCACCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCAGCTGTT GGAACTGCTA ATAAGTCAAC AGTGGAAGGC 
ATCCAGGCCT CGGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCCCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC AATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGATAAGTG TAAAGTGGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTG GCTGGGGCAA CCAGCATTGC TCGCCGGGCC 
AACCGAATCC TGTTAGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCTTC TGATGAATTG AGTAAAACCA TTTCTCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAC CCTGGTCTGC AAAAGAGCTT CCTGGACTCG 
GGATATCGGA TCCTCGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAACCT 
GACTTCCCTC CTCCTCCACC AGACATTGAT CAGCTCCGTT TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CCCTGCCCGA GGGTGAGGTC CCTCCACCCA GACCTCCACC ACCAGAAGAG 
AAAGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGTATGGC TCTACTGATG GCTGAGATGT CTCGACTGGT TAGAGGAGGC 
AGTGGTACCA AGAGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA AGTTGCAAAG CAGTGCACGG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG GATCCCAACC ATAAGCACCC AGCTCAAAAT TCTGTCCACT 
GTAAAGGCCA CCATGCTGGG TCGGACCAAC ATCAGCGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TCCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGTGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCAT GGTACCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_004680759.2 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_004680816.1 Condylura cristata vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCo230954
Clone ID Related Accession (Same CDS sequence)	XM_004680759.2
Accession Version	XM_004680759.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567112.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2015 this sequence version replaced XM_004680759.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACTCG CACCATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGT GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCAGCC GTGAGCAATC TTGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCGAGGG GTATCCTCTC TGGCACATCA 
GACCTACTTC TCACCTTCGA TGAGGCTGAG GTTCGGAAAA TTATTCGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACCAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAACTGACCC ACCAGGAGCA CCGAGTCATG TTGGTAAACT CAATGAATAC TGTGAAAGAG 
TTATTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATAGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC ATGGGCCAGT 
AAAGATACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCTAAATT GAACCAAGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCACT CCAGGGGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CCTCCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTGCGAGCC 
AGAGGACAAG GAGCCTCACC CATGGCCATG CAGAAAGCCC AGCAGGTATC TCAGGGTCTG 
GATATGCTCA CAGCAAAAGT GGAAAATGCT GCCCGGAAAC TGGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCTCAGAATT GGCTTGCAGA CCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ACCCTAAAGA GAGAGATGAC ATTCTACGTT CTCTTGGAGA AATATCTGCT 
TTGACTAATA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAAGTGGC CACAGCCCTG CAGAACATGC AGACTAAAAC CAATCGGGCT 
GTGGCCAATA GCAGACCCAC CAAAGCAGCA GTCCACCTTG AAGGCAAGAT TGAACAAGCA 
CAGCGATGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGACCTTA TCGTCAAGAT 
CTTCTTGCAA AGTGTGACAG AGTGGATCAG CTGACAGCAC AGCTGGCTGA TCTGGCTGCC 
CGAGGGGAAG GAGAGAGCCC ACAGGCACGA GCACTTGCCT CTCAGCTCCA AGACTCTTTG 
AAGGATCTGA AAACACGGAT GCAGGAAGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGTTAGCA GTGGCAGCCA CAGCACCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCAGCTGTT GGAACTGCTA ATAAGTCAAC AGTGGAAGGC 
ATCCAGGCCT CGGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCCCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC AATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGATAAGTG TAAAGTGGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTG GCTGGGGCAA CCAGCATTGC TCGCCGGGCC 
AACCGAATCC TGTTAGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCTTC TGATGAATTG AGTAAAACCA TTTCTCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAC CCTGGTCTGC AAAAGAGCTT CCTGGACTCG 
GGATATCGGA TCCTCGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAACCT 
GACTTCCCTC CTCCTCCACC AGACATTGAT CAGCTCCGTT TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CCCTGCCCGA GGGTGAGGTC CCTCCACCCA GACCTCCACC ACCAGAAGAG 
AAAGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG TTTTCCAGCC 
ACTGAGGTAG GTATAGGTGT TGTGGCAGAG GCAGATGCGG CCGATGCTGT TGGTTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATACC ATCCAATCAG 
CCAGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC CACCAAATGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGTA TGGCTCTACT GATGGCTGAG 
ATGTCTCGAC TGGTTAGAGG AGGCAGTGGT ACCAAGAGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAAGTTGC AAAGCAGTGC 
ACGGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGGATCCC AACCATAAGC 
ACCCAGCTCA AAATTCTGTC CACTGTAAAG GCCACCATGC TGGGTCGGAC CAACATCAGC 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTCCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG TGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCATGGTACC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004680816.1
CDS	86..3490
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNATPGDAGEQAIRQILDEAGKVGELCAGKERREILGTSKMLGQMTDQVADLRARGQGASPMAMQKAQQVSQGLDMLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTNKLADLRRQGKGDSPEARALAKQVATALQNMQTKTNRAVANSRPTKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKTRMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDIDQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGFPATEVGIGVVAEADAADAVGFPVPPDMEDDYEPELLLIPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_004680759.2
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004680759.2

ORF Insert Sequence:

ATGCCGGTGT TTCATACTCG CACCATCGAG AGCATCCTGG AGCCAGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGT GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCAGCC GTGAGCAATC TTGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA ACTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCGAGGG GTATCCTCTC TGGCACATCA 
GACCTACTTC TCACCTTCGA TGAGGCTGAG GTTCGGAAAA TTATTCGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACCAAG ATGGCCAAAA TGATTGATGA GAGACAGCAG 
GAACTGACCC ACCAGGAGCA CCGAGTCATG TTGGTAAACT CAATGAATAC TGTGAAAGAG 
TTATTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATAGA AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATTCGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC ATGGGCCAGT 
AAAGATACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCTAAATT GAACCAAGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCACT CCAGGGGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CCTCCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTGCGAGCC 
AGAGGACAAG GAGCCTCACC CATGGCCATG CAGAAAGCCC AGCAGGTATC TCAGGGTCTG 
GATATGCTCA CAGCAAAAGT GGAAAATGCT GCCCGGAAAC TGGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCT GCTCAGAATT GGCTTGCAGA CCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ACCCTAAAGA GAGAGATGAC ATTCTACGTT CTCTTGGAGA AATATCTGCT 
TTGACTAATA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAAGTGGC CACAGCCCTG CAGAACATGC AGACTAAAAC CAATCGGGCT 
GTGGCCAATA GCAGACCCAC CAAAGCAGCA GTCCACCTTG AAGGCAAGAT TGAACAAGCA 
CAGCGATGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGACCTTA TCGTCAAGAT 
CTTCTTGCAA AGTGTGACAG AGTGGATCAG CTGACAGCAC AGCTGGCTGA TCTGGCTGCC 
CGAGGGGAAG GAGAGAGCCC ACAGGCACGA GCACTTGCCT CTCAGCTCCA AGACTCTTTG 
AAGGATCTGA AAACACGGAT GCAGGAAGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGTTAGCA GTGGCAGCCA CAGCACCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGAGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCAGCTGTT GGAACTGCTA ATAAGTCAAC AGTGGAAGGC 
ATCCAGGCCT CGGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC AGCTGCCCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC AATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGATAAGTG TAAAGTGGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTG GCTGGGGCAA CCAGCATTGC TCGCCGGGCC 
AACCGAATCC TGTTAGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCTTC TGATGAATTG AGTAAAACCA TTTCTCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCTGGAAA CATTTCTGAC CCTGGTCTGC AAAAGAGCTT CCTGGACTCG 
GGATATCGGA TCCTCGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAACCT 
GACTTCCCTC CTCCTCCACC AGACATTGAT CAGCTCCGTT TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CCCTGCCCGA GGGTGAGGTC CCTCCACCCA GACCTCCACC ACCAGAAGAG 
AAAGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG TTTTCCAGCC 
ACTGAGGTAG GTATAGGTGT TGTGGCAGAG GCAGATGCGG CCGATGCTGT TGGTTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATACC ATCCAATCAG 
CCAGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC CACCAAATGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGTA TGGCTCTACT GATGGCTGAG 
ATGTCTCGAC TGGTTAGAGG AGGCAGTGGT ACCAAGAGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAAGTTGC AAAGCAGTGC 
ACGGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGGATCCC AACCATAAGC 
ACCCAGCTCA AAATTCTGTC CACTGTAAAG GCCACCATGC TGGGTCGGAC CAACATCAGC 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTCCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG TGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCATGGTACC AATAG