JAK1 cDNA ORF clone, Condylura cristata(star-nosed mole)

Gene
Clones
gRNAs

Summary

Gene Symbol	JAK1
Entrez Gene ID	101618899
Full Name	Janus kinase 1
Gene Type	protein-coding
Organism	Condylura cristata(star-nosed mole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012722225.1	XP_012577679.1	tyrosine-protein kinase JAK1
XM_004679513.2	XP_004679570.1	tyrosine-protein kinase JAK1

Pathways from BioSystems
Gene Ontology

The following JAK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the JAK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo228751	XM_012722225.1 Latest version!	Condylura cristata Janus kinase 1 (JAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCo228751	XM_004679513.2 Latest version!	Condylura cristata Janus kinase 1 (JAK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

JAK1 ( XM_012722225.1 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_012577679.1 Condylura cristata Janus kinase 1 (JAK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCo228751
Clone ID Related Accession (Same CDS sequence)	XM_012722225.1 , XM_004679513.2
Accession Version	XM_012722225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	tyrosine-protein kinase JAK1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567109.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTATC TAAATATAAA GGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGATGGAGGT GAATCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGCTCTTC 
TATCTCTCTG ACAGGGAGCC CCTCCGACTG GGCAGCGGAG AGTATACAGC GGAGGACCTG 
TGCATCAGGG CTGCACAGAA ATGCAGTATT TCTCCTTTGT GTCACAACCT CTTTGCTCTC 
TATGATGATA ACACCAAGCT CTGGTATGCT CCAAATCGTT CCATCACTGT TGATGACAAA 
ACATGGCTCC GGCTCCACTA TCGCATGAGG TTCTATTTCA CCAACTGGCA TGGGACCAAT 
GATAATGAGC AGTCAGTATG GCGACATTCT CCAAAGAAGC AGAAAAATGG CTATGAGAAA 
AAAAAAGTTC CAGATGCCAC CCCTCTCCTT GATGCCAGCT CTTTAGAGTA TCTTTTTGCT 
CAGGGACAGC ACGACTTGGT CAAATGCTTG GCTCCCATTC GAGACCCCAA GACTGAGCAG 
GATGGACATG ATATTGAGAA TGAGTGTCTG GGAATGGCTG TCCTGGCTAT TTCACATTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTTCCCAAGG ACATCAGTTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA ATCCATCAGG CAGAGGAACC TTCTTACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATCTG TGACAGCAGT 
GTGTCTACAC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TATGGTGCTG AAATATTTGA GACATCCATG TTACTGATTT CATCAGAAAA TGAAATGAAT 
CGCTTTCATG CAAATGACAG TGGAAACATT ATCTACTATG AAGTGATGGT AACTGGAAAT 
CTTGGAATCC AGTGGAGGCA GAAACCAAAT GTTGTTCCTG TTGAAAAGGA AAAAAAACTG 
AAACGGAAAA AACTGGAAAG TAAACATAAG AAGGATGAAG AGAAAAACAG AATCCGGGAA 
GAGTGGAATA ATTTCTCTTA CTTCCCTGAA ATCACTCACA TTGTAATAAA AGAATCTGTG 
GTCAGCATTA ATAAGCAGGA CAACAAAAAA ATGGAACTGA AACTCTCTTC CCATGAGGAG 
GCCTTGTCCT TTGTGTCCCT GGTGGATGGC TATTTCCGAC TCACAGCAGA TGCCCACCAT 
TACCTCTGCA CCGATGTGGC TCCTCCATTG ATCGTCCACA ACATACAGAA TGGCTGTCAT 
GGCCCAATTT GCACAGAGTA TGCCATCAAT AAATTACGGC AAGAAGGAAG CGAGGAGGGA 
ATGTATGTGT TGCGGTGGAG CTGCACTGAC TTTGACAACA TCCTCATGAC TGTCACCTGC 
TTTGAAAAGT CAGAGGTTCT GGGTGGCCAG AAACAGTTCA AGAACTTTCA GATTGAGGTA 
CAGAAGGGCT GCTATAGCCT GCACGGCTCA GACAGCAGCT TCCCCAGTCT AAAAGATCTC 
ATGGGCCACC TCAAGAAGCA GATCCTGCGC ACGGACAACA TCAGTTTTGT GCTGAAGCGC 
TGCTGCCAGC CCAAGCCCCG AGAAATCTCC AATCTGCTGG TGGCTACTAA GAAAGCCCAG 
GAATGGCAGC CTGTCTACCC CATGAGCCAG CTGAGTTTTG ATCGGATCCT CAAAAAAGAC 
ATTGTGCAAG GTGAACACCT TGGGAGAGGC ACTCGGACAC ACATCTATTC TGGGACCCTT 
TTGGATTACA AAGATGATGA AGGGACAGCT GAAGAGAAGA GAATAAAAGT GATCCTCAAA 
GTCTTAGACC CTAGCCATAG GGACATCTCT TTGGCCTTCT TTGAAGCAGC CAGCATGATG 
AGACAAGTCT CCCACAAACA CATTGTGTAC CTCTATGGTG TCTGTGTACG AGATGTGGAG 
AATATCATGG TGGAAGAGTT TGTGGAGGGG GGTCCCCTGG ATCTCTTCAT GCACCGGAAA 
AGTGATGTTC TCACCACTCC ATGGAAGTTC AAAGTGGCTA AACAGCTAGC CAGCGCCCTG 
AGTTACTTAG AGGATAAGGA CCTGGTCCAT GGAAATGTGT GCACTAAAAA CCTCCTCCTT 
GCCCGGGAGG GCATTGACAG CGAGTGTGGT CCGTTCATCA AGCTCAGTGA CCCTGGCATC 
CCCATCACTG TGCTGTCCAG GCAAGAGTGC ATAGAACGAA TCCCATGGAT TGCTCCAGAG 
TTTGTTGAAG ACTCCAAGAA CTTGAGTGTG GCTGCTGACA AATGGAGCTT TGGAACCACA 
CTCTGGGAAA TCTGCTACAA TGGCGAGATC CCCCTGAAAG ACAAGACACT CATTGAGAAA 
GAGAGATTCT ATGAAAGCCG CTGCAGACCG GTGACACCAT CTTGTAAAGA ATTGGCTGAC 
CTTATGACCC GCTGCATGAA CTATGATCCC AATCAGAGAC CCTTTTTTCG AGCCATCATG 
AGAGATATCA ACAAGCTGGA GGAGCAGAAT CCAGACATTG TTTCAGAAAA GAAGCCAGCC 
ACCGAAGTGG ATCCCACACA TTTTGAAAAG CGCTTCTTGA AGAAGATCCG TGATTTGGGA 
GAGGGCCACT TCGGGAAGGT TGAGCTCTGC AGGTATGACC CTGAGGGCGA CAATACAGGG 
GAGCAGGTGG CTGTGAAATC CCTGAAACCT GAAAGCGGAG GTAACCACAT AGCGGATCTG 
AAGAAGGAAA TCGAAATCTT AAGGAACCTG TATCATGAGA ACATTGTGAA GTACAAAGGC 
ATCTGCACAG AAGACGGAGG AAATGGTATT AAACTCATCA TGGAATTTCT GCCTTCTGGA 
AGCCTTAAGG AGTATCTTCC AAAAAATAAG AACAAAATCA ACCTCAAACA GCAGTTAAAA 
TATGCTGTTC AGATTTGTAA GGGTATGGAC TACTTGGGTT CTCGGCAGTA TGTTCACCGG 
GATTTAGCAG CAAGAAATGT CCTTGTTGAG AGTGAACACC AAGTGAAAAT TGGAGATTTT 
GGCTTAACCA AAGCAATTGA AACTGATAAG GAATACTACA CGGTCAAAGA TGATCGGGAT 
AGCCCTGTGT TTTGGTATGC TCCAGAATGT TTAATTCAGT GTAAATTTTA TATTGCCTCG 
GACGTTTGGT CTTTTGGAGT TACTCTGCAT GAGCTGCTTA CGTATTGTGA TTCAGATTCC 
AGTCCCATGG CATTGTTCCT GAAAATGATA GGTCCAACTC ATGGCCAAAT GACTGTAACA 
AGACTTGTGA ACACATTAAA AGATGGAAAA CGTTTGCCTT GTCCACCTAA CTGTCCAGAT 
GAGATTTACC AACTTATGAG AAAATGCTGG GATCTCCAAC CAGCAAATCG GACAACTTTT 
CAGAACCTTA TTGAAGGATT TGAAGCACTT TTAAAACAAC TACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012577679.1
CDS	462..3929
Translation	MQYLNIKEDCNAMAFCAKMRSSKKMEVNLEAPEPGVEVLFYLSDREPLRLGSGEYTAEDLCIRAAQKCSISPLCHNLFALYDDNTKLWYAPNRSITVDDKTWLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKVPDATPLLDASSLEYLFAQGQHDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNRFHANDSGNIIYYEVMVTGNLGIQWRQKPNVVPVEKEKKLKRKKLESKHKKDEEKNRIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEVLGGQKQFKNFQIEVQKGCYSLHGSDSSFPSLKDLMGHLKKQILRTDNISFVLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDIVQGEHLGRGTRTHIYSGTLLDYKDDEGTAEEKRIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPEFVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKKIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLIQCKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKDGKRLPCPPNCPDEIYQLMRKCWDLQPANRTTFQNLIEGFEALLKQLP

Target ORF information:

RefSeq Version	XM_012722225.1
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata Janus kinase 1 (JAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012722225.1

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA GGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGATGGAGGT GAATCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGCTCTTC 
TATCTCTCTG ACAGGGAGCC CCTCCGACTG GGCAGCGGAG AGTATACAGC GGAGGACCTG 
TGCATCAGGG CTGCACAGAA ATGCAGTATT TCTCCTTTGT GTCACAACCT CTTTGCTCTC 
TATGATGATA ACACCAAGCT CTGGTATGCT CCAAATCGTT CCATCACTGT TGATGACAAA 
ACATGGCTCC GGCTCCACTA TCGCATGAGG TTCTATTTCA CCAACTGGCA TGGGACCAAT 
GATAATGAGC AGTCAGTATG GCGACATTCT CCAAAGAAGC AGAAAAATGG CTATGAGAAA 
AAAAAAGTTC CAGATGCCAC CCCTCTCCTT GATGCCAGCT CTTTAGAGTA TCTTTTTGCT 
CAGGGACAGC ACGACTTGGT CAAATGCTTG GCTCCCATTC GAGACCCCAA GACTGAGCAG 
GATGGACATG ATATTGAGAA TGAGTGTCTG GGAATGGCTG TCCTGGCTAT TTCACATTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTTCCCAAGG ACATCAGTTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA ATCCATCAGG CAGAGGAACC TTCTTACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATCTG TGACAGCAGT 
GTGTCTACAC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TATGGTGCTG AAATATTTGA GACATCCATG TTACTGATTT CATCAGAAAA TGAAATGAAT 
CGCTTTCATG CAAATGACAG TGGAAACATT ATCTACTATG AAGTGATGGT AACTGGAAAT 
CTTGGAATCC AGTGGAGGCA GAAACCAAAT GTTGTTCCTG TTGAAAAGGA AAAAAAACTG 
AAACGGAAAA AACTGGAAAG TAAACATAAG AAGGATGAAG AGAAAAACAG AATCCGGGAA 
GAGTGGAATA ATTTCTCTTA CTTCCCTGAA ATCACTCACA TTGTAATAAA AGAATCTGTG 
GTCAGCATTA ATAAGCAGGA CAACAAAAAA ATGGAACTGA AACTCTCTTC CCATGAGGAG 
GCCTTGTCCT TTGTGTCCCT GGTGGATGGC TATTTCCGAC TCACAGCAGA TGCCCACCAT 
TACCTCTGCA CCGATGTGGC TCCTCCATTG ATCGTCCACA ACATACAGAA TGGCTGTCAT 
GGCCCAATTT GCACAGAGTA TGCCATCAAT AAATTACGGC AAGAAGGAAG CGAGGAGGGA 
ATGTATGTGT TGCGGTGGAG CTGCACTGAC TTTGACAACA TCCTCATGAC TGTCACCTGC 
TTTGAAAAGT CAGAGGTTCT GGGTGGCCAG AAACAGTTCA AGAACTTTCA GATTGAGGTA 
CAGAAGGGCT GCTATAGCCT GCACGGCTCA GACAGCAGCT TCCCCAGTCT AAAAGATCTC 
ATGGGCCACC TCAAGAAGCA GATCCTGCGC ACGGACAACA TCAGTTTTGT GCTGAAGCGC 
TGCTGCCAGC CCAAGCCCCG AGAAATCTCC AATCTGCTGG TGGCTACTAA GAAAGCCCAG 
GAATGGCAGC CTGTCTACCC CATGAGCCAG CTGAGTTTTG ATCGGATCCT CAAAAAAGAC 
ATTGTGCAAG GTGAACACCT TGGGAGAGGC ACTCGGACAC ACATCTATTC TGGGACCCTT 
TTGGATTACA AAGATGATGA AGGGACAGCT GAAGAGAAGA GAATAAAAGT GATCCTCAAA 
GTCTTAGACC CTAGCCATAG GGACATCTCT TTGGCCTTCT TTGAAGCAGC CAGCATGATG 
AGACAAGTCT CCCACAAACA CATTGTGTAC CTCTATGGTG TCTGTGTACG AGATGTGGAG 
AATATCATGG TGGAAGAGTT TGTGGAGGGG GGTCCCCTGG ATCTCTTCAT GCACCGGAAA 
AGTGATGTTC TCACCACTCC ATGGAAGTTC AAAGTGGCTA AACAGCTAGC CAGCGCCCTG 
AGTTACTTAG AGGATAAGGA CCTGGTCCAT GGAAATGTGT GCACTAAAAA CCTCCTCCTT 
GCCCGGGAGG GCATTGACAG CGAGTGTGGT CCGTTCATCA AGCTCAGTGA CCCTGGCATC 
CCCATCACTG TGCTGTCCAG GCAAGAGTGC ATAGAACGAA TCCCATGGAT TGCTCCAGAG 
TTTGTTGAAG ACTCCAAGAA CTTGAGTGTG GCTGCTGACA AATGGAGCTT TGGAACCACA 
CTCTGGGAAA TCTGCTACAA TGGCGAGATC CCCCTGAAAG ACAAGACACT CATTGAGAAA 
GAGAGATTCT ATGAAAGCCG CTGCAGACCG GTGACACCAT CTTGTAAAGA ATTGGCTGAC 
CTTATGACCC GCTGCATGAA CTATGATCCC AATCAGAGAC CCTTTTTTCG AGCCATCATG 
AGAGATATCA ACAAGCTGGA GGAGCAGAAT CCAGACATTG TTTCAGAAAA GAAGCCAGCC 
ACCGAAGTGG ATCCCACACA TTTTGAAAAG CGCTTCTTGA AGAAGATCCG TGATTTGGGA 
GAGGGCCACT TCGGGAAGGT TGAGCTCTGC AGGTATGACC CTGAGGGCGA CAATACAGGG 
GAGCAGGTGG CTGTGAAATC CCTGAAACCT GAAAGCGGAG GTAACCACAT AGCGGATCTG 
AAGAAGGAAA TCGAAATCTT AAGGAACCTG TATCATGAGA ACATTGTGAA GTACAAAGGC 
ATCTGCACAG AAGACGGAGG AAATGGTATT AAACTCATCA TGGAATTTCT GCCTTCTGGA 
AGCCTTAAGG AGTATCTTCC AAAAAATAAG AACAAAATCA ACCTCAAACA GCAGTTAAAA 
TATGCTGTTC AGATTTGTAA GGGTATGGAC TACTTGGGTT CTCGGCAGTA TGTTCACCGG 
GATTTAGCAG CAAGAAATGT CCTTGTTGAG AGTGAACACC AAGTGAAAAT TGGAGATTTT 
GGCTTAACCA AAGCAATTGA AACTGATAAG GAATACTACA CGGTCAAAGA TGATCGGGAT 
AGCCCTGTGT TTTGGTATGC TCCAGAATGT TTAATTCAGT GTAAATTTTA TATTGCCTCG 
GACGTTTGGT CTTTTGGAGT TACTCTGCAT GAGCTGCTTA CGTATTGTGA TTCAGATTCC 
AGTCCCATGG CATTGTTCCT GAAAATGATA GGTCCAACTC ATGGCCAAAT GACTGTAACA 
AGACTTGTGA ACACATTAAA AGATGGAAAA CGTTTGCCTT GTCCACCTAA CTGTCCAGAT 
GAGATTTACC AACTTATGAG AAAATGCTGG GATCTCCAAC CAGCAAATCG GACAACTTTT 
CAGAACCTTA TTGAAGGATT TGAAGCACTT TTAAAACAAC TACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JAK1 ( XM_004679513.2 ) cDNA ORF clone, Condylura cristata(star-nosed mole) -> XP_004679570.1 Condylura cristata Janus kinase 1 (JAK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCo228751
Clone ID Related Accession (Same CDS sequence)	XM_012722225.1 , XM_004679513.2
Accession Version	XM_004679513.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-09
Organism	Condylura cristata(star-nosed mole)
Product	tyrosine-protein kinase JAK1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004567109.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2015 this sequence version replaced XM_004679513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Condylura cristata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTATC TAAATATAAA GGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGATGGAGGT GAATCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGCTCTTC 
TATCTCTCTG ACAGGGAGCC CCTCCGACTG GGCAGCGGAG AGTATACAGC GGAGGACCTG 
TGCATCAGGG CTGCACAGAA ATGCAGTATT TCTCCTTTGT GTCACAACCT CTTTGCTCTC 
TATGATGATA ACACCAAGCT CTGGTATGCT CCAAATCGTT CCATCACTGT TGATGACAAA 
ACATGGCTCC GGCTCCACTA TCGCATGAGG TTCTATTTCA CCAACTGGCA TGGGACCAAT 
GATAATGAGC AGTCAGTATG GCGACATTCT CCAAAGAAGC AGAAAAATGG CTATGAGAAA 
AAAAAAGTTC CAGATGCCAC CCCTCTCCTT GATGCCAGCT CTTTAGAGTA TCTTTTTGCT 
CAGGGACAGC ACGACTTGGT CAAATGCTTG GCTCCCATTC GAGACCCCAA GACTGAGCAG 
GATGGACATG ATATTGAGAA TGAGTGTCTG GGAATGGCTG TCCTGGCTAT TTCACATTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTTCCCAAGG ACATCAGTTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA ATCCATCAGG CAGAGGAACC TTCTTACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATCTG TGACAGCAGT 
GTGTCTACAC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TATGGTGCTG AAATATTTGA GACATCCATG TTACTGATTT CATCAGAAAA TGAAATGAAT 
CGCTTTCATG CAAATGACAG TGGAAACATT ATCTACTATG AAGTGATGGT AACTGGAAAT 
CTTGGAATCC AGTGGAGGCA GAAACCAAAT GTTGTTCCTG TTGAAAAGGA AAAAAAACTG 
AAACGGAAAA AACTGGAAAG TAAACATAAG AAGGATGAAG AGAAAAACAG AATCCGGGAA 
GAGTGGAATA ATTTCTCTTA CTTCCCTGAA ATCACTCACA TTGTAATAAA AGAATCTGTG 
GTCAGCATTA ATAAGCAGGA CAACAAAAAA ATGGAACTGA AACTCTCTTC CCATGAGGAG 
GCCTTGTCCT TTGTGTCCCT GGTGGATGGC TATTTCCGAC TCACAGCAGA TGCCCACCAT 
TACCTCTGCA CCGATGTGGC TCCTCCATTG ATCGTCCACA ACATACAGAA TGGCTGTCAT 
GGCCCAATTT GCACAGAGTA TGCCATCAAT AAATTACGGC AAGAAGGAAG CGAGGAGGGA 
ATGTATGTGT TGCGGTGGAG CTGCACTGAC TTTGACAACA TCCTCATGAC TGTCACCTGC 
TTTGAAAAGT CAGAGGTTCT GGGTGGCCAG AAACAGTTCA AGAACTTTCA GATTGAGGTA 
CAGAAGGGCT GCTATAGCCT GCACGGCTCA GACAGCAGCT TCCCCAGTCT AAAAGATCTC 
ATGGGCCACC TCAAGAAGCA GATCCTGCGC ACGGACAACA TCAGTTTTGT GCTGAAGCGC 
TGCTGCCAGC CCAAGCCCCG AGAAATCTCC AATCTGCTGG TGGCTACTAA GAAAGCCCAG 
GAATGGCAGC CTGTCTACCC CATGAGCCAG CTGAGTTTTG ATCGGATCCT CAAAAAAGAC 
ATTGTGCAAG GTGAACACCT TGGGAGAGGC ACTCGGACAC ACATCTATTC TGGGACCCTT 
TTGGATTACA AAGATGATGA AGGGACAGCT GAAGAGAAGA GAATAAAAGT GATCCTCAAA 
GTCTTAGACC CTAGCCATAG GGACATCTCT TTGGCCTTCT TTGAAGCAGC CAGCATGATG 
AGACAAGTCT CCCACAAACA CATTGTGTAC CTCTATGGTG TCTGTGTACG AGATGTGGAG 
AATATCATGG TGGAAGAGTT TGTGGAGGGG GGTCCCCTGG ATCTCTTCAT GCACCGGAAA 
AGTGATGTTC TCACCACTCC ATGGAAGTTC AAAGTGGCTA AACAGCTAGC CAGCGCCCTG 
AGTTACTTAG AGGATAAGGA CCTGGTCCAT GGAAATGTGT GCACTAAAAA CCTCCTCCTT 
GCCCGGGAGG GCATTGACAG CGAGTGTGGT CCGTTCATCA AGCTCAGTGA CCCTGGCATC 
CCCATCACTG TGCTGTCCAG GCAAGAGTGC ATAGAACGAA TCCCATGGAT TGCTCCAGAG 
TTTGTTGAAG ACTCCAAGAA CTTGAGTGTG GCTGCTGACA AATGGAGCTT TGGAACCACA 
CTCTGGGAAA TCTGCTACAA TGGCGAGATC CCCCTGAAAG ACAAGACACT CATTGAGAAA 
GAGAGATTCT ATGAAAGCCG CTGCAGACCG GTGACACCAT CTTGTAAAGA ATTGGCTGAC 
CTTATGACCC GCTGCATGAA CTATGATCCC AATCAGAGAC CCTTTTTTCG AGCCATCATG 
AGAGATATCA ACAAGCTGGA GGAGCAGAAT CCAGACATTG TTTCAGAAAA GAAGCCAGCC 
ACCGAAGTGG ATCCCACACA TTTTGAAAAG CGCTTCTTGA AGAAGATCCG TGATTTGGGA 
GAGGGCCACT TCGGGAAGGT TGAGCTCTGC AGGTATGACC CTGAGGGCGA CAATACAGGG 
GAGCAGGTGG CTGTGAAATC CCTGAAACCT GAAAGCGGAG GTAACCACAT AGCGGATCTG 
AAGAAGGAAA TCGAAATCTT AAGGAACCTG TATCATGAGA ACATTGTGAA GTACAAAGGC 
ATCTGCACAG AAGACGGAGG AAATGGTATT AAACTCATCA TGGAATTTCT GCCTTCTGGA 
AGCCTTAAGG AGTATCTTCC AAAAAATAAG AACAAAATCA ACCTCAAACA GCAGTTAAAA 
TATGCTGTTC AGATTTGTAA GGGTATGGAC TACTTGGGTT CTCGGCAGTA TGTTCACCGG 
GATTTAGCAG CAAGAAATGT CCTTGTTGAG AGTGAACACC AAGTGAAAAT TGGAGATTTT 
GGCTTAACCA AAGCAATTGA AACTGATAAG GAATACTACA CGGTCAAAGA TGATCGGGAT 
AGCCCTGTGT TTTGGTATGC TCCAGAATGT TTAATTCAGT GTAAATTTTA TATTGCCTCG 
GACGTTTGGT CTTTTGGAGT TACTCTGCAT GAGCTGCTTA CGTATTGTGA TTCAGATTCC 
AGTCCCATGG CATTGTTCCT GAAAATGATA GGTCCAACTC ATGGCCAAAT GACTGTAACA 
AGACTTGTGA ACACATTAAA AGATGGAAAA CGTTTGCCTT GTCCACCTAA CTGTCCAGAT 
GAGATTTACC AACTTATGAG AAAATGCTGG GATCTCCAAC CAGCAAATCG GACAACTTTT 
CAGAACCTTA TTGAAGGATT TGAAGCACTT TTAAAACAAC TACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004679570.1
CDS	301..3768
Translation	MQYLNIKEDCNAMAFCAKMRSSKKMEVNLEAPEPGVEVLFYLSDREPLRLGSGEYTAEDLCIRAAQKCSISPLCHNLFALYDDNTKLWYAPNRSITVDDKTWLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKVPDATPLLDASSLEYLFAQGQHDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNRFHANDSGNIIYYEVMVTGNLGIQWRQKPNVVPVEKEKKLKRKKLESKHKKDEEKNRIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEVLGGQKQFKNFQIEVQKGCYSLHGSDSSFPSLKDLMGHLKKQILRTDNISFVLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDIVQGEHLGRGTRTHIYSGTLLDYKDDEGTAEEKRIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPEFVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKKIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLIQCKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKDGKRLPCPPNCPDEIYQLMRKCWDLQPANRTTFQNLIEGFEALLKQLP

Target ORF information:

RefSeq Version	XM_004679513.2
Organism	Condylura cristata(star-nosed mole)
Definition	Condylura cristata Janus kinase 1 (JAK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004679513.2

ORF Insert Sequence:

ATGCAGTATC TAAATATAAA GGAGGACTGC AATGCCATGG CTTTCTGTGC TAAAATGAGG 
AGCTCCAAGA AGATGGAGGT GAATCTGGAG GCCCCTGAGC CAGGGGTGGA AGTGCTCTTC 
TATCTCTCTG ACAGGGAGCC CCTCCGACTG GGCAGCGGAG AGTATACAGC GGAGGACCTG 
TGCATCAGGG CTGCACAGAA ATGCAGTATT TCTCCTTTGT GTCACAACCT CTTTGCTCTC 
TATGATGATA ACACCAAGCT CTGGTATGCT CCAAATCGTT CCATCACTGT TGATGACAAA 
ACATGGCTCC GGCTCCACTA TCGCATGAGG TTCTATTTCA CCAACTGGCA TGGGACCAAT 
GATAATGAGC AGTCAGTATG GCGACATTCT CCAAAGAAGC AGAAAAATGG CTATGAGAAA 
AAAAAAGTTC CAGATGCCAC CCCTCTCCTT GATGCCAGCT CTTTAGAGTA TCTTTTTGCT 
CAGGGACAGC ACGACTTGGT CAAATGCTTG GCTCCCATTC GAGACCCCAA GACTGAGCAG 
GATGGACATG ATATTGAGAA TGAGTGTCTG GGAATGGCTG TCCTGGCTAT TTCACATTAT 
GCCATGATGA AGAAGATGCA GTTGCCAGAA CTTCCCAAGG ACATCAGTTA CAAGCGATAT 
ATTCCAGAAA CATTGAATAA ATCCATCAGG CAGAGGAACC TTCTTACCAG GATGCGGATA 
AATAATGTTT TCAAGGATTT CCTAAAGGAA TTTAACAACA AGACCATCTG TGACAGCAGT 
GTGTCTACAC ATGACCTGAA GGTGAAATAC TTGGCTACCT TGGAAACTTT GACAAAACAT 
TATGGTGCTG AAATATTTGA GACATCCATG TTACTGATTT CATCAGAAAA TGAAATGAAT 
CGCTTTCATG CAAATGACAG TGGAAACATT ATCTACTATG AAGTGATGGT AACTGGAAAT 
CTTGGAATCC AGTGGAGGCA GAAACCAAAT GTTGTTCCTG TTGAAAAGGA AAAAAAACTG 
AAACGGAAAA AACTGGAAAG TAAACATAAG AAGGATGAAG AGAAAAACAG AATCCGGGAA 
GAGTGGAATA ATTTCTCTTA CTTCCCTGAA ATCACTCACA TTGTAATAAA AGAATCTGTG 
GTCAGCATTA ATAAGCAGGA CAACAAAAAA ATGGAACTGA AACTCTCTTC CCATGAGGAG 
GCCTTGTCCT TTGTGTCCCT GGTGGATGGC TATTTCCGAC TCACAGCAGA TGCCCACCAT 
TACCTCTGCA CCGATGTGGC TCCTCCATTG ATCGTCCACA ACATACAGAA TGGCTGTCAT 
GGCCCAATTT GCACAGAGTA TGCCATCAAT AAATTACGGC AAGAAGGAAG CGAGGAGGGA 
ATGTATGTGT TGCGGTGGAG CTGCACTGAC TTTGACAACA TCCTCATGAC TGTCACCTGC 
TTTGAAAAGT CAGAGGTTCT GGGTGGCCAG AAACAGTTCA AGAACTTTCA GATTGAGGTA 
CAGAAGGGCT GCTATAGCCT GCACGGCTCA GACAGCAGCT TCCCCAGTCT AAAAGATCTC 
ATGGGCCACC TCAAGAAGCA GATCCTGCGC ACGGACAACA TCAGTTTTGT GCTGAAGCGC 
TGCTGCCAGC CCAAGCCCCG AGAAATCTCC AATCTGCTGG TGGCTACTAA GAAAGCCCAG 
GAATGGCAGC CTGTCTACCC CATGAGCCAG CTGAGTTTTG ATCGGATCCT CAAAAAAGAC 
ATTGTGCAAG GTGAACACCT TGGGAGAGGC ACTCGGACAC ACATCTATTC TGGGACCCTT 
TTGGATTACA AAGATGATGA AGGGACAGCT GAAGAGAAGA GAATAAAAGT GATCCTCAAA 
GTCTTAGACC CTAGCCATAG GGACATCTCT TTGGCCTTCT TTGAAGCAGC CAGCATGATG 
AGACAAGTCT CCCACAAACA CATTGTGTAC CTCTATGGTG TCTGTGTACG AGATGTGGAG 
AATATCATGG TGGAAGAGTT TGTGGAGGGG GGTCCCCTGG ATCTCTTCAT GCACCGGAAA 
AGTGATGTTC TCACCACTCC ATGGAAGTTC AAAGTGGCTA AACAGCTAGC CAGCGCCCTG 
AGTTACTTAG AGGATAAGGA CCTGGTCCAT GGAAATGTGT GCACTAAAAA CCTCCTCCTT 
GCCCGGGAGG GCATTGACAG CGAGTGTGGT CCGTTCATCA AGCTCAGTGA CCCTGGCATC 
CCCATCACTG TGCTGTCCAG GCAAGAGTGC ATAGAACGAA TCCCATGGAT TGCTCCAGAG 
TTTGTTGAAG ACTCCAAGAA CTTGAGTGTG GCTGCTGACA AATGGAGCTT TGGAACCACA 
CTCTGGGAAA TCTGCTACAA TGGCGAGATC CCCCTGAAAG ACAAGACACT CATTGAGAAA 
GAGAGATTCT ATGAAAGCCG CTGCAGACCG GTGACACCAT CTTGTAAAGA ATTGGCTGAC 
CTTATGACCC GCTGCATGAA CTATGATCCC AATCAGAGAC CCTTTTTTCG AGCCATCATG 
AGAGATATCA ACAAGCTGGA GGAGCAGAAT CCAGACATTG TTTCAGAAAA GAAGCCAGCC 
ACCGAAGTGG ATCCCACACA TTTTGAAAAG CGCTTCTTGA AGAAGATCCG TGATTTGGGA 
GAGGGCCACT TCGGGAAGGT TGAGCTCTGC AGGTATGACC CTGAGGGCGA CAATACAGGG 
GAGCAGGTGG CTGTGAAATC CCTGAAACCT GAAAGCGGAG GTAACCACAT AGCGGATCTG 
AAGAAGGAAA TCGAAATCTT AAGGAACCTG TATCATGAGA ACATTGTGAA GTACAAAGGC 
ATCTGCACAG AAGACGGAGG AAATGGTATT AAACTCATCA TGGAATTTCT GCCTTCTGGA 
AGCCTTAAGG AGTATCTTCC AAAAAATAAG AACAAAATCA ACCTCAAACA GCAGTTAAAA 
TATGCTGTTC AGATTTGTAA GGGTATGGAC TACTTGGGTT CTCGGCAGTA TGTTCACCGG 
GATTTAGCAG CAAGAAATGT CCTTGTTGAG AGTGAACACC AAGTGAAAAT TGGAGATTTT 
GGCTTAACCA AAGCAATTGA AACTGATAAG GAATACTACA CGGTCAAAGA TGATCGGGAT 
AGCCCTGTGT TTTGGTATGC TCCAGAATGT TTAATTCAGT GTAAATTTTA TATTGCCTCG 
GACGTTTGGT CTTTTGGAGT TACTCTGCAT GAGCTGCTTA CGTATTGTGA TTCAGATTCC 
AGTCCCATGG CATTGTTCCT GAAAATGATA GGTCCAACTC ATGGCCAAAT GACTGTAACA 
AGACTTGTGA ACACATTAAA AGATGGAAAA CGTTTGCCTT GTCCACCTAA CTGTCCAGAT 
GAGATTTACC AACTTATGAG AAAATGCTGG GATCTCCAAC CAGCAAATCG GACAACTTTT 
CAGAACCTTA TTGAAGGATT TGAAGCACTT TTAAAACAAC TACCATGA