SPAST cDNA ORF clone, Colius striatus(speckled mousebird)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAST
Entrez Gene ID	104564184
Full Name	spastin
Gene Type	protein-coding
Organism	Colius striatus(speckled mousebird)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010210895.1	XP_010209197.1	spastin

Pathways from BioSystems
Gene Ontology

The following SPAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo159037	XM_010210895.1 Latest version!	Colius striatus spastin (SPAST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAST ( XM_010210895.1 ) cDNA ORF clone, Colius striatus(speckled mousebird) -> XP_010209197.1 Colius striatus spastin (SPAST), mRNA.

Price & Availability↑

CloneID	OCo159037
Clone ID Related Accession (Same CDS sequence)	XM_010210895.1
Accession Version	XM_010210895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-06
Organism	Colius striatus(speckled mousebird)
Product	spastin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010670406.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Colius striatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGC GCATCGACGA AGACGAGAGA GCAGGACAAA AGGAACAAGC TGTTGAATGG 
TATAAGAAAG GAATTGAAGA ACTGGAAAAA GGAATAGCTG TCTTAGTAAT TGGTCAAGGT 
GATCAGTGTG AACGGGCTCG ACGTCTGCAA TCTAAAATGA TGACAAATTT GGCAATGGCC 
AAGGATCGCT TGCAGCTTTT AGAGAAGCTG CAGGCAGTTT TGCAAATTTC CAAGCCGCAA 
ATGGAGGTCT ATAATGACAG TACTAACCTG GCATGCCGCA ATGGACATCT CCAGTCAGAA 
AGTGGAGCCG TTCCAAAAAA GAAGGATCCC TTAACACACA CAAGTAATTC CCTTCCTCGT 
TCAAAAACTG TTGCAAAAAC TGGATCCACA GGCCTTTCAA GTCACCACAG AACACCTAGC 
TACAGTGGAA TATCAACTTC TGCATCTAGA CCTCCTGCAG CTTCAACTTA TAAGCCTGCT 
CCTAAAAATA ATAGAACAAA TAAGCCTTCC ACTCCTACCA CTGCCCCTCG AAAAAAGAAA 
GACTTGAAGA TATTTAGAAA TGTGGACAGT AACCTTGCTA ATCTCATCCT GAATGAAATT 
GTTGATAGTG GGCCAGCTGT CAAATTTGAT GATATTGCAG GGCAGGAGCT GGCTAAACAA 
GCTTTGCAAG AAATTGTTAT CCTTCCATCT CTCAGACCTG AGTTATTTAC AGGACTTAGA 
GCTCCTGCAC GTGGTTTATT GCTGTTTGGC CCCCCAGGAA ATGGGAAGAC AATGTTGGCC 
AAAGCAGTTG CTGCAGAGTC AAATGCTACT TTCTTTAATA TAAGTGCAGC AAGCCTAACT 
TCAAAATATG TAGGAGAAGG GGAGAAACTA GTACGTGCTC TATTTGCAGT AGCCAGAGAA 
CTTCAGCCTT CTATAATATT TATTGATGAA GTTGATAGCC TTTTGTGTGA AAGACGAGAA 
GGTGAACATG ATGCTAGTAG GCGTCTAAAA ACAGAATTTT TAATAGAATT TGATGGAGTA 
CAGTCTTCTG GAGAGGACAG AATACTTGTG ATGGGAGCAA CAAACCGGCC GCAGGAGCTT 
GATGATGCTG TTCTCAGACG ATTCACTAAA CGGATATACG TGTCTTTACC AAATGAGGAA 
ACAAGACTGA TTTTGCTGAA GAATCTTCTA AGCAAGCAAG GAAGTCCTTT GACCCAAAAA 
GAGTTGGCAC AACTAGCTAG AATGACAGAT GGATACTCTG GGAGTGACTT AACTGCATTA 
GCAAAGGATG CAGCGCTGGG CCCTATTCGA GAATTAAAAC CAGAACAAGT GAAGAACATG 
TCTGCCAGTG AGATGAGAAA TATTAAACTA TCAGATTTCA CTGAGTCTTT GAAGAAGATA 
AAGCGCAGTT TGAGCCCTCA GACCCTGGAA GCATATATTC GCTGGAACAA GGACTTTGGA 
GATACCACGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010209197.1
CDS	46..1500
Translation	MALRIDEDERAGQKEQAVEWYKKGIEELEKGIAVLVIGQGDQCERARRLQSKMMTNLAMAKDRLQLLEKLQAVLQISKPQMEVYNDSTNLACRNGHLQSESGAVPKKKDPLTHTSNSLPRSKTVAKTGSTGLSSHHRTPSYSGISTSASRPPAASTYKPAPKNNRTNKPSTPTTAPRKKKDLKIFRNVDSNLANLILNEIVDSGPAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSSGEDRILVMGATNRPQELDDAVLRRFTKRIYVSLPNEETRLILLKNLLSKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIKLSDFTESLKKIKRSLSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_010210895.1
Organism	Colius striatus(speckled mousebird)
Definition	Colius striatus spastin (SPAST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010210895.1

ORF Insert Sequence:

ATGGCGCTGC GCATCGACGA AGACGAGAGA GCAGGACAAA AGGAACAAGC TGTTGAATGG 
TATAAGAAAG GAATTGAAGA ACTGGAAAAA GGAATAGCTG TCTTAGTAAT TGGTCAAGGT 
GATCAGTGTG AACGGGCTCG ACGTCTGCAA TCTAAAATGA TGACAAATTT GGCAATGGCC 
AAGGATCGCT TGCAGCTTTT AGAGAAGCTG CAGGCAGTTT TGCAAATTTC CAAGCCGCAA 
ATGGAGGTCT ATAATGACAG TACTAACCTG GCATGCCGCA ATGGACATCT CCAGTCAGAA 
AGTGGAGCCG TTCCAAAAAA GAAGGATCCC TTAACACACA CAAGTAATTC CCTTCCTCGT 
TCAAAAACTG TTGCAAAAAC TGGATCCACA GGCCTTTCAA GTCACCACAG AACACCTAGC 
TACAGTGGAA TATCAACTTC TGCATCTAGA CCTCCTGCAG CTTCAACTTA TAAGCCTGCT 
CCTAAAAATA ATAGAACAAA TAAGCCTTCC ACTCCTACCA CTGCCCCTCG AAAAAAGAAA 
GACTTGAAGA TATTTAGAAA TGTGGACAGT AACCTTGCTA ATCTCATCCT GAATGAAATT 
GTTGATAGTG GGCCAGCTGT CAAATTTGAT GATATTGCAG GGCAGGAGCT GGCTAAACAA 
GCTTTGCAAG AAATTGTTAT CCTTCCATCT CTCAGACCTG AGTTATTTAC AGGACTTAGA 
GCTCCTGCAC GTGGTTTATT GCTGTTTGGC CCCCCAGGAA ATGGGAAGAC AATGTTGGCC 
AAAGCAGTTG CTGCAGAGTC AAATGCTACT TTCTTTAATA TAAGTGCAGC AAGCCTAACT 
TCAAAATATG TAGGAGAAGG GGAGAAACTA GTACGTGCTC TATTTGCAGT AGCCAGAGAA 
CTTCAGCCTT CTATAATATT TATTGATGAA GTTGATAGCC TTTTGTGTGA AAGACGAGAA 
GGTGAACATG ATGCTAGTAG GCGTCTAAAA ACAGAATTTT TAATAGAATT TGATGGAGTA 
CAGTCTTCTG GAGAGGACAG AATACTTGTG ATGGGAGCAA CAAACCGGCC GCAGGAGCTT 
GATGATGCTG TTCTCAGACG ATTCACTAAA CGGATATACG TGTCTTTACC AAATGAGGAA 
ACAAGACTGA TTTTGCTGAA GAATCTTCTA AGCAAGCAAG GAAGTCCTTT GACCCAAAAA 
GAGTTGGCAC AACTAGCTAG AATGACAGAT GGATACTCTG GGAGTGACTT AACTGCATTA 
GCAAAGGATG CAGCGCTGGG CCCTATTCGA GAATTAAAAC CAGAACAAGT GAAGAACATG 
TCTGCCAGTG AGATGAGAAA TATTAAACTA TCAGATTTCA CTGAGTCTTT GAAGAAGATA 
AAGCGCAGTT TGAGCCCTCA GACCCTGGAA GCATATATTC GCTGGAACAA GGACTTTGGA 
GATACCACGG TGTGA