CcE1D cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CcE1D
Entrez Gene ID	101453851
Full Name	esterase 1 clade D
Synonyms	ESTF
Gene Type	protein-coding
Organism	Ceratitis capitata(Mediterranean fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320377.1	NP_001307306.1	esterase 1 clade D precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A genomic perspective to assessing quality of mass-reared SIT flies used in Mediterranean fruit fly (Ceratitis capitata) eradication in California.
Calla B, Hall B, Hou S, Geib SM
BMC genomics1598(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CcE1D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CcE1D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe76193	NM_001320377.1 Latest version!	Ceratitis capitata esterase 1 clade D (CcE1D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CcE1D ( NM_001320377.1 ) cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly) -> NP_001307306.1 Ceratitis capitata esterase 1 clade D (CcE1D), mRNA.

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CloneID	OCe76193
Clone ID Related Accession (Same CDS sequence)	NM_001320377.1
Accession Version	NM_001320377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-27
Organism	Ceratitis capitata(Mediterranean fruit fly)
Product	esterase 1 clade D precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AOHK02001032.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GAMC01017692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02055688, SAMN02208095 [ECO:0000348] ##Evidence-Data-END##

ATGTCTAGTC TGAAATTGAG TTTATTTCAT AATTTATTTG TATGCTTTAT TTTATTTAAT 
TGTCTAATAA ATGACAGCTT TGCTGATACT GAAAATGCTG ATGAGACAGT TGAAGTAAAA 
TTTCAGAATG AAGAACGAAA TATTACAATA ATCGGTGTAC GTAACGAGAG TTTTAACGCT 
AAAGAATATT ATGCATTTCG TGGCATACGG TATGTAAAGC CGCCCGTAAA TGAGCTGAGA 
TTTAAGGATC CTCAGCCACT CACTCAGCTG CCATTAACCA TCGACGCCCG CTACGATGGA 
CATGACTGTC CTTCAATTTA TGCTACAAAT GCCTCCGAGG ATTGTCTCAC ACTCAATATT 
TATATGCCGA CGACCATAGA AGCCGCTGCG TTACCTGTAT TAGTCTTCAT ACATCCCGGT 
GGCTTGTATG TTGGTTCGGC GCTTAGTAAT TTCATTGGCC CTGCTTATCT GCTCTCGAAA 
CCACTTGTAG TGGTCACTTT CAATTATCGT CTTGGCACAC TTGGTTTCCT ACAACTCGGC 
ACACGTGAGA TACCGGGTAA TGCGGGTTTC AAAGATCAAG TGTATGCCTT GCGTTGGGTT 
GAGAAATATA TTGCACACTT TGGTGGTAAC CCTAAGGATA TTACGCTCAT GGGCTACAGC 
GCTGGTGCAT TGAGTGTTAG TTTGCATTTG GTGTCGCCTA TGTCGCGTAA TCTCTTCCAA 
AAAGCTATAA TAATGAGTGG CTCATTACCA CCTCAAACAG TGTTGCCGCA GCATTCACAG 
TTGGAGCTGT TGCGCAAACA AGCTCGTGTG CTCCAATGTA CGGGCGTCAT GAATGAAGTG 
GAGATTTTAA ATTGTTTGGC CACTTTTAGT GGCACTGAAA TTGCGGCAAC CCTACGTAAG 
CTATTTGTTT TCGGCAAAGA TAATCCAATT TACATTTATC TGCCAGTTAT TGAAAGAGAT 
TATGGACAAG AACGCTTCTT AGTTGAAGAT CCTTACGAAA GTCTGCAACA TGGCGCGTTT 
TCGAAATTGC CCATTTTAAT TGGTTTTACA AATGGAGAAT TTTGTCAATC AGCTGTGGAC 
ATCTTCAAAG ACACCAAGTT GGTTCAGCAT TTCTTTGAAG AGTTTGAAAA TGTGGCGCCA 
GAAATTTTTA TGTACGCCAA TCATGACGGA AATTTTAGTA TGATCAATGA GGTGCTGAAA 
AATTATTATC TACGAAATTC AGAAGAGTTG ACGCGAGTTA ACCTACCGCG TTTATGCGAT 
TTTTTCTCAG ATGCAATTAT TCGCTTTGGT GCACATCGCT TGGTGGAGTT AGTTGCACCG 
TATACTAAAG TGTTTCCGTA CAGCTTTGAG TTCAGAGGAG AGTTTAGCAA TTTGGATTAT 
CCCCTAAGAC CAGCCCGCGT TGAGCATATG GACGATTTAA TGTATTTATT TGAAATGCCA 
TCAAGTAACT TTAGTCGTAC GGAGTCCAAT CTAGATATGA TCCGGGGATA TACGAAGTTC 
GTATATGATT TCGTTAAAGA AGGTGATAGG CTTTTTGGGA AAATACCAAC TTATCCAGCT 
GGCTACACTA AAATTTATCG TGATTTGGAA TTCCAACCTG GCACGAGTTT CCCCATGGAC 
ACCTACGAGA TGTGGAAGGA TTTATTTCTA ACTTCAAAAC GAAAGAAAAA TAAGAAAAAT 
AAACGGATAC AAGATTTACA AGCAGAACCG AGTGTCGGAA TTAAAAATGT GATTGACAGT 
CAAATATTGT TGAAAAATTT TGTTCTTTAC TTATTTTCAA ACTATTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307306.1
CDS	134..1924
Translation	MSSLKLSLFHNLFVCFILFNCLINDSFADTENADETVEVKFQNEERNITIIGVRNESFNAKEYYAFRGIRYVKPPVNELRFKDPQPLTQLPLTIDARYDGHDCPSIYATNASEDCLTLNIYMPTTIEAAALPVLVFIHPGGLYVGSALSNFIGPAYLLSKPLVVVTFNYRLGTLGFLQLGTREIPGNAGFKDQVYALRWVEKYIAHFGGNPKDITLMGYSAGALSVSLHLVSPMSRNLFQKAIIMSGSLPPQTVLPQHSQLELLRKQARVLQCTGVMNEVEILNCLATFSGTEIAATLRKLFVFGKDNPIYIYLPVIERDYGQERFLVEDPYESLQHGAFSKLPILIGFTNGEFCQSAVDIFKDTKLVQHFFEEFENVAPEIFMYANHDGNFSMINEVLKNYYLRNSEELTRVNLPRLCDFFSDAIIRFGAHRLVELVAPYTKVFPYSFEFRGEFSNLDYPLRPARVEHMDDLMYLFEMPSSNFSRTESNLDMIRGYTKFVYDFVKEGDRLFGKIPTYPAGYTKIYRDLEFQPGTSFPMDTYEMWKDLFLTSKRKKNKKNKRIQDLQAEPSVGIKNVIDSQILLKNFVLYLFSNYL

Target ORF information:

RefSeq Version	NM_001320377.1
Organism	Ceratitis capitata(Mediterranean fruit fly)
Definition	Ceratitis capitata esterase 1 clade D (CcE1D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320377.1

ORF Insert Sequence:

ATGTCTAGTC TGAAATTGAG TTTATTTCAT AATTTATTTG TATGCTTTAT TTTATTTAAT 
TGTCTAATAA ATGACAGCTT TGCTGATACT GAAAATGCTG ATGAGACAGT TGAAGTAAAA 
TTTCAGAATG AAGAACGAAA TATTACAATA ATCGGTGTAC GTAACGAGAG TTTTAACGCT 
AAAGAATATT ATGCATTTCG TGGCATACGG TATGTAAAGC CGCCCGTAAA TGAGCTGAGA 
TTTAAGGATC CTCAGCCACT CACTCAGCTG CCATTAACCA TCGACGCCCG CTACGATGGA 
CATGACTGTC CTTCAATTTA TGCTACAAAT GCCTCCGAGG ATTGTCTCAC ACTCAATATT 
TATATGCCGA CGACCATAGA AGCCGCTGCG TTACCTGTAT TAGTCTTCAT ACATCCCGGT 
GGCTTGTATG TTGGTTCGGC GCTTAGTAAT TTCATTGGCC CTGCTTATCT GCTCTCGAAA 
CCACTTGTAG TGGTCACTTT CAATTATCGT CTTGGCACAC TTGGTTTCCT ACAACTCGGC 
ACACGTGAGA TACCGGGTAA TGCGGGTTTC AAAGATCAAG TGTATGCCTT GCGTTGGGTT 
GAGAAATATA TTGCACACTT TGGTGGTAAC CCTAAGGATA TTACGCTCAT GGGCTACAGC 
GCTGGTGCAT TGAGTGTTAG TTTGCATTTG GTGTCGCCTA TGTCGCGTAA TCTCTTCCAA 
AAAGCTATAA TAATGAGTGG CTCATTACCA CCTCAAACAG TGTTGCCGCA GCATTCACAG 
TTGGAGCTGT TGCGCAAACA AGCTCGTGTG CTCCAATGTA CGGGCGTCAT GAATGAAGTG 
GAGATTTTAA ATTGTTTGGC CACTTTTAGT GGCACTGAAA TTGCGGCAAC CCTACGTAAG 
CTATTTGTTT TCGGCAAAGA TAATCCAATT TACATTTATC TGCCAGTTAT TGAAAGAGAT 
TATGGACAAG AACGCTTCTT AGTTGAAGAT CCTTACGAAA GTCTGCAACA TGGCGCGTTT 
TCGAAATTGC CCATTTTAAT TGGTTTTACA AATGGAGAAT TTTGTCAATC AGCTGTGGAC 
ATCTTCAAAG ACACCAAGTT GGTTCAGCAT TTCTTTGAAG AGTTTGAAAA TGTGGCGCCA 
GAAATTTTTA TGTACGCCAA TCATGACGGA AATTTTAGTA TGATCAATGA GGTGCTGAAA 
AATTATTATC TACGAAATTC AGAAGAGTTG ACGCGAGTTA ACCTACCGCG TTTATGCGAT 
TTTTTCTCAG ATGCAATTAT TCGCTTTGGT GCACATCGCT TGGTGGAGTT AGTTGCACCG 
TATACTAAAG TGTTTCCGTA CAGCTTTGAG TTCAGAGGAG AGTTTAGCAA TTTGGATTAT 
CCCCTAAGAC CAGCCCGCGT TGAGCATATG GACGATTTAA TGTATTTATT TGAAATGCCA 
TCAAGTAACT TTAGTCGTAC GGAGTCCAAT CTAGATATGA TCCGGGGATA TACGAAGTTC 
GTATATGATT TCGTTAAAGA AGGTGATAGG CTTTTTGGGA AAATACCAAC TTATCCAGCT 
GGCTACACTA AAATTTATCG TGATTTGGAA TTCCAACCTG GCACGAGTTT CCCCATGGAC 
ACCTACGAGA TGTGGAAGGA TTTATTTCTA ACTTCAAAAC GAAAGAAAAA TAAGAAAAAT 
AAACGGATAC AAGATTTACA AGCAGAACCG AGTGTCGGAA TTAAAAATGT GATTGACAGT 
CAAATATTGT TGAAAAATTT TGTTCTTTAC TTATTTTCAA ACTATTTATG A