Il10 cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	Il10
Entrez Gene ID	100715618
Full Name	interleukin 10
Synonyms	CSIF,IL-10
Gene Type	protein-coding
Organism	Cavia porcellus(domestic guinea pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260485.1	NP_001247414.1	interleukin-10 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Molecular cloning and expression of the IL-10 gene from guinea pigs.
Dirisala VR, Jeevan A, Bix G, Yoshimura T, McMurray DN
Gene498(1)120-7(2012 Apr)

Spontaneous cytokine gene expression in normal guinea pig blood and tissues.
Scarozza AM, Ramsingh AI, Wicher V, Wicher K
Cytokine10(11)851-9(1998 Nov)

Cytokine gene expression in skin of susceptible guinea-pig infected with Treponema pallidum.
Wicher V, Scarozza AM, Ramsingh AI, Wicher K
Immunology95(2)242-7(1998 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The complete open reading frame consists of 537 base pairs which encodes a protein of 179 amino acids. This cDNA sequence exhibited 87% homology with human IL-10.
Title: Molecular cloning and expression of the IL-10 gene from guinea pigs.

The following Il10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Il10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODo00216	NM_001260485.1 Latest version!	Cavia porcellus interleukin 10 (Il10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Il10 ( NM_001260485.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> NP_001247414.1 Cavia porcellus interleukin 10 (Il10), mRNA.

Price & Availability↑

CloneID	ODo00216
Clone ID Related Accession (Same CDS sequence)	NM_001260485.1
Accession Version	NM_001260485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Cavia porcellus(domestic guinea pig)
Product	interleukin-10 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JN020146.1. On May 7, 2012 this sequence version replaced XM_003474663.1. ##Evidence-Data-START## Transcript exon combination :: JN020146.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02693769, SAMN02693772 [ECO:0000348] ##Evidence-Data-END##

ATGCCCAGCT CAGCGCTGCT ATGTTGCCTG GTCTTACTGG CCGGGGTCAA GGCCAGCCAA 
GGCACGAACA CCCAGTCTGA GGACAGCTGT GCCCACTTCC CAGCCGGCCT GCCCCACATG 
CTTCGAGAGC TCCGAGCTGC CTTTGGCAGG GTGAAGACTT TCTTTCAAAC ACAGGATCAG 
CTGGACAACG TGCTGTTAAA CAAGTCATTG CTGGAGGATT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CAGAGATGAT CCAGTTTTAT CTAGTGGAGG TGATGCCCAA GGCAGAAAAT 
CATGACCCAG ACATCAAGGA ACATGTAAGC TCCCTGGGGG AAAAGCTGAA GACCCTCAGG 
CTGCGGCTGC GACGCTGTCA TCGATTTCTT CCCTGTGAAA ACAAGAGCAA GGCGGTGGAG 
CAGGTGAAAA ATACGTTTAA TAAGCTCCAA GAGAAAGGCG TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAGCC TACATGACAA GGAAATTAAC AAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247414.1
CDS	70..606
Translation	MPSSALLCCLVLLAGVKASQGTNTQSEDSCAHFPAGLPHMLRELRAAFGRVKTFFQTQDQLDNVLLNKSLLEDFKGYLGCQALSEMIQFYLVEVMPKAENHDPDIKEHVSSLGEKLKTLRLRLRRCHRFLPCENKSKAVEQVKNTFNKLQEKGVYKAMSEFDIFINYIEAYMTRKLTN

Target ORF information:

RefSeq Version	NM_001260485.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus interleukin 10 (Il10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260485.1

ORF Insert Sequence:

ATGCCCAGCT CAGCGCTGCT ATGTTGCCTG GTCTTACTGG CCGGGGTCAA GGCCAGCCAA 
GGCACGAACA CCCAGTCTGA GGACAGCTGT GCCCACTTCC CAGCCGGCCT GCCCCACATG 
CTTCGAGAGC TCCGAGCTGC CTTTGGCAGG GTGAAGACTT TCTTTCAAAC ACAGGATCAG 
CTGGACAACG TGCTGTTAAA CAAGTCATTG CTGGAGGATT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CAGAGATGAT CCAGTTTTAT CTAGTGGAGG TGATGCCCAA GGCAGAAAAT 
CATGACCCAG ACATCAAGGA ACATGTAAGC TCCCTGGGGG AAAAGCTGAA GACCCTCAGG 
CTGCGGCTGC GACGCTGTCA TCGATTTCTT CCCTGTGAAA ACAAGAGCAA GGCGGTGGAG 
CAGGTGAAAA ATACGTTTAA TAAGCTCCAA GAGAAAGGCG TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAGCC TACATGACAA GGAAATTAAC AAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular cloning and expression of the IL-10 gene from guinea pigs.
Gene498(1)120-7(2012 Apr)
Dirisala VR,Jeevan A,Bix G,Yoshimura T,McMurray DN

Spontaneous cytokine gene expression in normal guinea pig blood and tissues.
Cytokine10(11)851-9(1998 Nov)
Scarozza AM,Ramsingh AI,Wicher V,Wicher K

Cytokine gene expression in skin of susceptible guinea-pig infected with Treponema pallidum.
Immunology95(2)242-7(1998 Oct)
Wicher V,Scarozza AM,Ramsingh AI,Wicher K