IARS2 cDNA ORF clone, Cariama cristata(Red-legged seriema)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS2
Entrez Gene ID	104164221
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Cariama cristata(Red-legged seriema)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009703484.1	XP_009701786.1	isoleucine--tRNA ligase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following IARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa459838	XM_009703484.1 Latest version!	Cariama cristata isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS2 ( XM_009703484.1 ) cDNA ORF clone, Cariama cristata(Red-legged seriema) -> XP_009701786.1 Cariama cristata isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

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CloneID	OCa459838
Clone ID Related Accession (Same CDS sequence)	XM_009703484.1
Accession Version	XM_009703484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Cariama cristata(Red-legged seriema)
Product	isoleucine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009625116.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cariama cristata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAG GTTATAAAGT ACATTATGTG CCAGGATGGG ATTGTCACGG CCTGCCAATT 
GAGTTGAAGG CATTGTCAGA AGTCAAAGGA GCTGAAAATC TTTCACCAAT GGAAATTCGA 
CAGAGAGCCA GAGAATTTGC AGAAAGAGCA ATTGAGAAAC AGAAATCTGC TTTCATTCGT 
TGGGGTGTGA TGGCAGATTG GGCTAACAGC TACCGTACAT TTGATCCAAA GTATGAAGCA 
AACCAGCTGA GAGTCTTCTA TAAAATGTAT GATAAGGGTT TCATTTATCA GGACTATAAA 
CCTGTGTTCT GGTCCCCTTC AGCAAAAACA GCCTTGGCTG AAGCAGAGCT TGAATACAAT 
GAGCAGCATG TCAGTCGTTC TGTGTATATG AAATTTCCCC TGTTAAAGTC ACCACCTAAG 
CTGACTTCTG TGATAGATGG ATCATCCCCT GCTAGTCTGC TGGTCTGGAC CACACAACCT 
TGGACTGTGC CAGCCAATCA AGCAGTTTGT TACATGCCAG ATTCGACATA CTCAGTTGTG 
AAATGTACAA CTACTGGTGA ACACTTCATT CTAGCTGCAG ATAGAGTTGA ATCTACAGCT 
GCTGCTTTGG ACACGCAGTT TGAGGTTATA TCAACATGTA AAGGTGTAGA TTTGGTAGAT 
GGTTCTTGTG CTCATCCCAC AATTCCTGGC AGAGTCTCTC CTCTGTTACC TGCAAATCAC 
GTGACAATGG CAAAAGGAAC AGGATTAGTT CATACAGCCC CAGCTCATGG TATGGAAGAC 
TACAGCGTAG CTTCCCACCA TCAGCTACCC ACGGATTGCC TTGTGGATGA AGGTGGGTTT 
TTTACTGAAG CTGCAGGTCC TGAGCTTAAA AACAAGAATG TCCTTGAAGA GGGGAATGAA 
GCTGTCATTC AGATGCTTCA AGCAGCTGGG AGTTTGTTAA AAGAGGAGAA ATATGTGCAC 
AGTTACCCAT ATGACTGGAG GACTAAAAAA CCAATGATTA TTCGTGCCAG CAAACAGTGG 
TTTGTAAACA CGGCAGGTGT GAAGGCTACA GCACAGGAAG TGCTGAAAAA AGTGAAAGTT 
GTTCCTACGT CTGCAGTGAA CAGAATGCTT GAAATGCTGG ACAGAAGGAC GTTTTGGTGT 
ATATCGCGGC AAAGATGTTG GGGTGTTCCG ATTCCTGTAT TTTATCAGAA GAACACAGGG 
GAATGCTTGA TAAACAGTGA AACTATCACA GATGTCATCA AAATAGTGGA ACAGCAAGGA 
TCAGATGCGT GGTGGACCCT TCCTGTTGAA CAACTACTAT CAAAAGAGGC TGTAGCGAAG 
GCTGGTGGTC GCGATGTTTT GGATTATGTC AAGGGACAGG ATGTCCTAGA CATCTGGTTT 
GACAGTGGAA CTTCCTGGGC TCATGTTTTG GAAGGTGCAG AGCAAAGAGC AGATACATAC 
CTTGAAGGAA AAGACCAGCT GGGAGGTTGG TTTCAATCCT CTCTACTAAC TAGTGTGGCA 
GCAAGGAAAA AAGCACCATA TAAGACTCTG GTAGTTCATG GATTTACTCT TGGTGAGAAA 
GGGGAGAAGA TGTCTAAATC TATTGGCAAC GTGGTTGACC CTGATGTAGT TATCAGTGGA 
GGTGAAGATC ACACCAAGGA TCCTCCATAC GGTGCCGATG TTCTCCGCTG GTGGGTGGCA 
GAATCAAATG TTTTTACTGA GGTGCTGATC GGGCCTGTTG TACTAAATGC TGCAAGAGAT 
GATATCAACA AGCTTCGGAA TACACTGCGC TTCATGCTGG GAAACATGGC TGGCTTCAAC 
CCAGAAACTG ATTCCATCCC TGCTTCAGAG ATGTACATAG TAGATCAATA CATGCTGCAT 
TTATTGCAAG ATTATGGCAG CAAGGTTACA GAAGCATACA AAGAATATGA TTATAGCAAA 
GTTGTTCGAT TATTGCAAGC ATTCTGTTCC AGAAACTTGT CTAACTTTTA TTTCAGCTTA 
ATCAAAGACA GACTCTATTG TGAAGAAGAA AAAGATCCTA AGCGTCGTTC ATGTCAAACT 
GTGCTAGCAG AGGCTTTAGA CGTAGTAGTC CGATCCTTTG CACCAATTCT TCCTCACTTG 
GCAGAGGAGG TTTTCCAGCA CCTCCCTTAT AAGAAAGGCT CAGAGGGTGT GTTTCGTACT 
GGCTGGATTA ATACAAGCTC AGCATGGAAG AAGCCTGGCA TTGAGGAAGC AATAGAAGGT 
GCCTGTGCAA TGAGAGACTC TTTTCTTGGT TCCATCTCTG GCAAGAACGC TTTGGAATAT 
GAAGTCGTCA TTGTAATTGA GCCTGGATTG CTGTTTGAAT TAATGGAGGC ACTGCAGGCT 
GAAGAAACTT CTAGTGTTTC ACAGCTGAAT GAAATCATGA TGGCTTCCCA GACAACCCTG 
TTGAGTGAGC TACCTAAAGA AACACCTACA GATGCAAACA TCACCAAAGG GACCTTTCTG 
ATTAACTTGG AAGGTGGTGA TATCCGTGAA GAGTCTTCAT ATAAAGTTAT AGCCCTACCA 
GTTGCCAAAG CAAAATGCCC TCGCTGCAGG AGATACACGT CCGATTCCTC AAGCACTCCC 
TGCCCACGGT GCCTGAAAGT CATTGCTGGA AAAGGGAACA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009701786.1
CDS	33..2657
Translation	MMRGYKVHYVPGWDCHGLPIELKALSEVKGAENLSPMEIRQRAREFAERAIEKQKSAFIRWGVMADWANSYRTFDPKYEANQLRVFYKMYDKGFIYQDYKPVFWSPSAKTALAEAELEYNEQHVSRSVYMKFPLLKSPPKLTSVIDGSSPASLLVWTTQPWTVPANQAVCYMPDSTYSVVKCTTTGEHFILAADRVESTAAALDTQFEVISTCKGVDLVDGSCAHPTIPGRVSPLLPANHVTMAKGTGLVHTAPAHGMEDYSVASHHQLPTDCLVDEGGFFTEAAGPELKNKNVLEEGNEAVIQMLQAAGSLLKEEKYVHSYPYDWRTKKPMIIRASKQWFVNTAGVKATAQEVLKKVKVVPTSAVNRMLEMLDRRTFWCISRQRCWGVPIPVFYQKNTGECLINSETITDVIKIVEQQGSDAWWTLPVEQLLSKEAVAKAGGRDVLDYVKGQDVLDIWFDSGTSWAHVLEGAEQRADTYLEGKDQLGGWFQSSLLTSVAARKKAPYKTLVVHGFTLGEKGEKMSKSIGNVVDPDVVISGGEDHTKDPPYGADVLRWWVAESNVFTEVLIGPVVLNAARDDINKLRNTLRFMLGNMAGFNPETDSIPASEMYIVDQYMLHLLQDYGSKVTEAYKEYDYSKVVRLLQAFCSRNLSNFYFSLIKDRLYCEEEKDPKRRSCQTVLAEALDVVVRSFAPILPHLAEEVFQHLPYKKGSEGVFRTGWINTSSAWKKPGIEEAIEGACAMRDSFLGSISGKNALEYEVVIVIEPGLLFELMEALQAEETSSVSQLNEIMMASQTTLLSELPKETPTDANITKGTFLINLEGGDIREESSYKVIALPVAKAKCPRCRRYTSDSSSTPCPRCLKVIAGKGNR

Target ORF information:

RefSeq Version	XM_009703484.1
Organism	Cariama cristata(Red-legged seriema)
Definition	Cariama cristata isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009703484.1

ORF Insert Sequence:

ATGATGAGAG GTTATAAAGT ACATTATGTG CCAGGATGGG ATTGTCACGG CCTGCCAATT 
GAGTTGAAGG CATTGTCAGA AGTCAAAGGA GCTGAAAATC TTTCACCAAT GGAAATTCGA 
CAGAGAGCCA GAGAATTTGC AGAAAGAGCA ATTGAGAAAC AGAAATCTGC TTTCATTCGT 
TGGGGTGTGA TGGCAGATTG GGCTAACAGC TACCGTACAT TTGATCCAAA GTATGAAGCA 
AACCAGCTGA GAGTCTTCTA TAAAATGTAT GATAAGGGTT TCATTTATCA GGACTATAAA 
CCTGTGTTCT GGTCCCCTTC AGCAAAAACA GCCTTGGCTG AAGCAGAGCT TGAATACAAT 
GAGCAGCATG TCAGTCGTTC TGTGTATATG AAATTTCCCC TGTTAAAGTC ACCACCTAAG 
CTGACTTCTG TGATAGATGG ATCATCCCCT GCTAGTCTGC TGGTCTGGAC CACACAACCT 
TGGACTGTGC CAGCCAATCA AGCAGTTTGT TACATGCCAG ATTCGACATA CTCAGTTGTG 
AAATGTACAA CTACTGGTGA ACACTTCATT CTAGCTGCAG ATAGAGTTGA ATCTACAGCT 
GCTGCTTTGG ACACGCAGTT TGAGGTTATA TCAACATGTA AAGGTGTAGA TTTGGTAGAT 
GGTTCTTGTG CTCATCCCAC AATTCCTGGC AGAGTCTCTC CTCTGTTACC TGCAAATCAC 
GTGACAATGG CAAAAGGAAC AGGATTAGTT CATACAGCCC CAGCTCATGG TATGGAAGAC 
TACAGCGTAG CTTCCCACCA TCAGCTACCC ACGGATTGCC TTGTGGATGA AGGTGGGTTT 
TTTACTGAAG CTGCAGGTCC TGAGCTTAAA AACAAGAATG TCCTTGAAGA GGGGAATGAA 
GCTGTCATTC AGATGCTTCA AGCAGCTGGG AGTTTGTTAA AAGAGGAGAA ATATGTGCAC 
AGTTACCCAT ATGACTGGAG GACTAAAAAA CCAATGATTA TTCGTGCCAG CAAACAGTGG 
TTTGTAAACA CGGCAGGTGT GAAGGCTACA GCACAGGAAG TGCTGAAAAA AGTGAAAGTT 
GTTCCTACGT CTGCAGTGAA CAGAATGCTT GAAATGCTGG ACAGAAGGAC GTTTTGGTGT 
ATATCGCGGC AAAGATGTTG GGGTGTTCCG ATTCCTGTAT TTTATCAGAA GAACACAGGG 
GAATGCTTGA TAAACAGTGA AACTATCACA GATGTCATCA AAATAGTGGA ACAGCAAGGA 
TCAGATGCGT GGTGGACCCT TCCTGTTGAA CAACTACTAT CAAAAGAGGC TGTAGCGAAG 
GCTGGTGGTC GCGATGTTTT GGATTATGTC AAGGGACAGG ATGTCCTAGA CATCTGGTTT 
GACAGTGGAA CTTCCTGGGC TCATGTTTTG GAAGGTGCAG AGCAAAGAGC AGATACATAC 
CTTGAAGGAA AAGACCAGCT GGGAGGTTGG TTTCAATCCT CTCTACTAAC TAGTGTGGCA 
GCAAGGAAAA AAGCACCATA TAAGACTCTG GTAGTTCATG GATTTACTCT TGGTGAGAAA 
GGGGAGAAGA TGTCTAAATC TATTGGCAAC GTGGTTGACC CTGATGTAGT TATCAGTGGA 
GGTGAAGATC ACACCAAGGA TCCTCCATAC GGTGCCGATG TTCTCCGCTG GTGGGTGGCA 
GAATCAAATG TTTTTACTGA GGTGCTGATC GGGCCTGTTG TACTAAATGC TGCAAGAGAT 
GATATCAACA AGCTTCGGAA TACACTGCGC TTCATGCTGG GAAACATGGC TGGCTTCAAC 
CCAGAAACTG ATTCCATCCC TGCTTCAGAG ATGTACATAG TAGATCAATA CATGCTGCAT 
TTATTGCAAG ATTATGGCAG CAAGGTTACA GAAGCATACA AAGAATATGA TTATAGCAAA 
GTTGTTCGAT TATTGCAAGC ATTCTGTTCC AGAAACTTGT CTAACTTTTA TTTCAGCTTA 
ATCAAAGACA GACTCTATTG TGAAGAAGAA AAAGATCCTA AGCGTCGTTC ATGTCAAACT 
GTGCTAGCAG AGGCTTTAGA CGTAGTAGTC CGATCCTTTG CACCAATTCT TCCTCACTTG 
GCAGAGGAGG TTTTCCAGCA CCTCCCTTAT AAGAAAGGCT CAGAGGGTGT GTTTCGTACT 
GGCTGGATTA ATACAAGCTC AGCATGGAAG AAGCCTGGCA TTGAGGAAGC AATAGAAGGT 
GCCTGTGCAA TGAGAGACTC TTTTCTTGGT TCCATCTCTG GCAAGAACGC TTTGGAATAT 
GAAGTCGTCA TTGTAATTGA GCCTGGATTG CTGTTTGAAT TAATGGAGGC ACTGCAGGCT 
GAAGAAACTT CTAGTGTTTC ACAGCTGAAT GAAATCATGA TGGCTTCCCA GACAACCCTG 
TTGAGTGAGC TACCTAAAGA AACACCTACA GATGCAAACA TCACCAAAGG GACCTTTCTG 
ATTAACTTGG AAGGTGGTGA TATCCGTGAA GAGTCTTCAT ATAAAGTTAT AGCCCTACCA 
GTTGCCAAAG CAAAATGCCC TCGCTGCAGG AGATACACGT CCGATTCCTC AAGCACTCCC 
TGCCCACGGT GCCTGAAAGT CATTGCTGGA AAAGGGAACA GGTGA