CMA1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CMA1
Entrez Gene ID	490628
Full Name	chymase 1
General protein information	Preferred Names chymase 1 Names chymase alpha-chymase chymase 1, mast cell mast cell chymase mast cell protease I serine protease
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013424.1	NP_001013442.1	chymase precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC540321	XP_002696732.1
Rattus norvegicus (Norway rat)	Cma1	NP_037224.1
Bos taurus (cattle)	LOC100139881	XP_002696686.1
Homo sapiens (human)	CMA1	NP_001827.1
Pan troglodytes (chimpanzee)	CMA1	XP_001170224.1
	CMA1	XP_001114321.1
Canis lupus familiaris (dog)	CMA1	NP_001013442.1
Mus musculus (house mouse)	Cma1	NP_034910.1

Gene Ontology
Bibliography

Related articles in PubMed

Effect of chymase inhibition on the arteriovenous fistula stenosis in dogs.
Jin D, Ueda H, Takai S, Okamoto Y, Muramatsu M, Sakaguchi M, Shibahara N, Katsuoka Y, Miyazaki M
Journal of the American Society of Nephrology : JASN16(4)1024-34(2005 Apr)

Cloning and expression of the dog mast cell alpha-chymase gene.
Caughey GH, Blount JL, Koerber KL, Kitamura M, Fang KC
Journal of immunology (Baltimore, Md. : 1950)159(9)4367-75(1997 Nov)

Dog mast cell chymase: molecular cloning and characterization.
Caughey GH, Raymond WW, Vanderslice P
Biochemistry29(21)5166-71(1990 May)

Purification and characterization of dog mastocytoma chymase: identification of an octapeptide conserved in chymotryptic leukocyte proteinases.
Caughey GH, Viro NF, Lazarus SC, Nadel JA
Biochimica et biophysica acta952(2)142-9(1988 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mast cell-derived chymase plays an essential role in pathogenesis of arteriovenous fistula access failure, and chymase inhibition may be therapeutic target for treatment of hemodialysis vascular access dysfunction.
Title: Effect of chymase inhibition on the arteriovenous fistula stenosis in dogs.

The following CMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00043	NM_001013424.1 Latest version!	Canis lupus familiaris chymase 1 (CMA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CMA1 ( NM_001013424.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001013442.1 Canis lupus familiaris chymase 1 (CMA1), mRNA.

Price & Availability↑

CloneID	OCe00043
Clone ID Related Accession (Same CDS sequence)	NM_001013424.1
Accession Version	NM_001013424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Canis lupus familiaris(dog)
Product	chymase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U89607.1. On Mar 25, 2005 this sequence version replaced XM_547750.1. ##Evidence-Data-START## Transcript exon combination :: J02904.1, DN377243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4547742, SAMN00013515 [ECO:0000348] ##Evidence-Data-END##

ATGCATTGTC TTCCTCTCAC CCTGCTGCTC CTTCTCCTAT GTTCCAGAGC AGAAGCTGAG 
GAGATCATCG GGGGCACAGA GTCCAAGCCA CACTCCCGCC CTTACATGGC CCACCTGGAA 
ATCCTCACTC TCCGGAATCA CCTGGCGTCT TGTGGTGGTT TCCTGATAAG GCGGAACTTC 
GTACTGACAG CTGCTCACTG TGCAGGAAGG TTCATAATGG TCACCCTGGG AGCCCATAAC 
ATACAAAAGA AAGAAGACAC GTGGCAGAAG CTTGAGGTCA TAAAACAATT CCCTCACCCA 
AAATATGATG ACCTTACTCT TCGCCATGAC ATCATGCTAC TGAAGTTGAA GGAGAAGGCC 
AACCTGACCC TGGCCGTGGG GACCCTCCCC CTCTCACCCC AGTTCAACTT CGTCCCACCT 
GGGAGAATGT GCCGGGTGGC TGGCTGGGGA AAAAGACAAG TCAATGGATC AGGCTCTGAC 
ACTCTGCAAG AGGTGAAGCT GAGGCTCATG GACCCCCAGG CCTGTAGACA CTACATGGCT 
TTTGACCACA ACCTCCAGCT GTGTGTTGGC AATCCCAGGA AGACAAAATC TGCATTTAAG 
GGAGATTCAG GGGGCCCTCT TCTGTGTGCT GGGGTAGCCC AGGGAATTGT GTCCTATGGG 
CAGAATGATG CAAAGCCCCC TGCTGTCTTC ACCCGAATCT CCCACTACCG GCCCTGGATC 
AATAAGGTTC TGAAGCAGAA TAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013442.1
CDS	32..781
Translation	MHCLPLTLLLLLLCSRAEAEEIIGGTESKPHSRPYMAHLEILTLRNHLASCGGFLIRRNFVLTAAHCAGRFIMVTLGAHNIQKKEDTWQKLEVIKQFPHPKYDDLTLRHDIMLLKLKEKANLTLAVGTLPLSPQFNFVPPGRMCRVAGWGKRQVNGSGSDTLQEVKLRLMDPQACRHYMAFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGQNDAKPPAVFTRISHYRPWINKVLKQNKA

Target ORF information:

RefSeq Version	NM_001013424.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris chymase 1 (CMA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013424.1

ORF Insert Sequence:

ATGCATTGTC TTCCTCTCAC CCTGCTGCTC CTTCTCCTAT GTTCCAGAGC AGAAGCTGAG 
GAGATCATCG GGGGCACAGA GTCCAAGCCA CACTCCCGCC CTTACATGGC CCACCTGGAA 
ATCCTCACTC TCCGGAATCA CCTGGCGTCT TGTGGTGGTT TCCTGATAAG GCGGAACTTC 
GTACTGACAG CTGCTCACTG TGCAGGAAGG TTCATAATGG TCACCCTGGG AGCCCATAAC 
ATACAAAAGA AAGAAGACAC GTGGCAGAAG CTTGAGGTCA TAAAACAATT CCCTCACCCA 
AAATATGATG ACCTTACTCT TCGCCATGAC ATCATGCTAC TGAAGTTGAA GGAGAAGGCC 
AACCTGACCC TGGCCGTGGG GACCCTCCCC CTCTCACCCC AGTTCAACTT CGTCCCACCT 
GGGAGAATGT GCCGGGTGGC TGGCTGGGGA AAAAGACAAG TCAATGGATC AGGCTCTGAC 
ACTCTGCAAG AGGTGAAGCT GAGGCTCATG GACCCCCAGG CCTGTAGACA CTACATGGCT 
TTTGACCACA ACCTCCAGCT GTGTGTTGGC AATCCCAGGA AGACAAAATC TGCATTTAAG 
GGAGATTCAG GGGGCCCTCT TCTGTGTGCT GGGGTAGCCC AGGGAATTGT GTCCTATGGG 
CAGAATGATG CAAAGCCCCC TGCTGTCTTC ACCCGAATCT CCCACTACCG GCCCTGGATC 
AATAAGGTTC TGAAGCAGAA TAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of chymase inhibition on the arteriovenous fistula stenosis in dogs.
Journal of the American Society of Nephrology : JASN16(4)1024-34(2005 Apr)
Jin D,Ueda H,Takai S,Okamoto Y,Muramatsu M,Sakaguchi M,Shibahara N,Katsuoka Y,Miyazaki M

Cloning and expression of the dog mast cell alpha-chymase gene.
Journal of immunology (Baltimore, Md. : 1950)159(9)4367-75(1997 Nov)
Caughey GH,Blount JL,Koerber KL,Kitamura M,Fang KC

Dog mast cell chymase: molecular cloning and characterization.
Biochemistry29(21)5166-71(1990 May)
Caughey GH,Raymond WW,Vanderslice P

Purification and characterization of dog mastocytoma chymase: identification of an octapeptide conserved in chymotryptic leukocyte proteinases.
Biochimica et biophysica acta952(2)142-9(1988 Jan)
Caughey GH,Viro NF,Lazarus SC,Nadel JA