MTCL1 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022421619.1	XP_022277327.1	microtubule cross-linking factor 1 isoform X1
XM_022421626.1	XP_022277334.1	microtubule cross-linking factor 1 isoform X8
XM_022421631.1	XP_022277339.1	microtubule cross-linking factor 1 isoform X14
XM_005623075.1	XP_005623132.1	microtubule cross-linking factor 1 isoform X1
XM_005623080.2	XP_005623137.1	microtubule cross-linking factor 1 isoform X3
XM_022421623.1	XP_022277331.1	microtubule cross-linking factor 1 isoform X5
XM_022421624.1	XP_022277332.1	microtubule cross-linking factor 1 isoform X6
XM_022421621.1	XP_022277329.1	microtubule cross-linking factor 1 isoform X3
XM_022421622.1	XP_022277330.1	microtubule cross-linking factor 1 isoform X4
XM_022421627.1	XP_022277335.1	microtubule cross-linking factor 1 isoform X9
XM_022421630.1	XP_022277338.1	microtubule cross-linking factor 1 isoform X12
XM_005623080.3	XP_005623137.1	microtubule cross-linking factor 1 isoform X13
XM_005623078.1	XP_005623135.1	microtubule cross-linking factor 1 isoform X2
XM_022421620.1	XP_022277328.1	microtubule cross-linking factor 1 isoform X2
XM_022421625.1	XP_022277333.1	microtubule cross-linking factor 1 isoform X7
XM_022421629.1	XP_022277337.1	microtubule cross-linking factor 1 isoform X11
XM_022421628.1	XP_022277336.1	microtubule cross-linking factor 1 isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe09959	XM_022421620.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe289879	XM_022421619.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe289880	XM_022421621.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe289881	XM_022421622.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe289882	XM_022421623.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe289883	XM_022421624.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe289884	XM_022421625.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe289885	XM_022421626.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe289886	XM_022421627.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe289887	XM_022421628.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe289888	XM_022421629.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe289889	XM_022421630.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe289890	XM_022421631.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe09960	XM_005623075.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe09963	XM_005623078.1 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe09965	XM_005623080.3 Latest version!	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OCe09965	XM_005623080.2	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

MTCL1 ( XM_022421620.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277328.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe09959
Clone ID Related Accession (Same CDS sequence)	XM_022421620.1
Accession Version	XM_022421620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA 
CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT 
CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA 
GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277328.1
CDS	117..5153
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQQSGGTERLGNCRSTTKTQRKLAPRRKDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421620.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421620.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA 
CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT 
CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA 
GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421619.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277327.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe289879
Clone ID Related Accession (Same CDS sequence)	XM_022421619.1
Accession Version	XM_022421619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
AGTGGTGGAA CCGAGCGCCT TGGAAACTGC AGGTCTACAA CCAAAACTCA AAGGAAACTG 
GCACCCCGGC GCAAGGATGA CAGTGCCGAT TTGAAGTGCC AGCTTCAGTT TGCCAAAGAG 
GAAGGCTCCC TGATGCGTAA AAAGATGGCC AAGCTGGGGA AGGAGAAGGA CGAGCTGGAA 
CAGGAGCTCC AGAAGTACAA GGCCCTCTAT GGAGACGTGG ACAGCCCCCT CCCCACGGGG 
GAAGCCGGGG GGCCGCCCAG CACCAGGGAG GCTGAGCTGA AGCTGCGCTT GAAGTTGGTG 
GAGGAAGAGG CCAACATCTT GGGCCGGAAG ATTGTGGAAC TGGAGGTGGA GAACCGAGGC 
CTCAAAGCAG AAATGGAGGA CATGAGGCTC CAGTACGAGC GAGAGGGTAC AGGCCGGGAC 
CACATCACAG GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC GACGGAGCTC 
CGCCGGCACC TGCAGTTCGT GGAGGAGGAA GCGGAGCTGC TCCGGAGGTC CATATCTGAG 
ATCGAAGACC ACAACCGGCA GCTGACCCAT GAGCTGAACA AGTTCAAGTT TGAGCCTCGG 
CCGGAGCCAG GGTGGCTGGC GGATGGCGCG GGCAAGGGCG TGGGCAGCGG GGCCCCTCTG 
CAGGAGGAGC TCAAGTCGGC ACGGCTACAG ATCAATGAGC TCAGTGGGAA GGTGCTGAAG 
CTGCAGTATG AGAACCGGGT GCTACTGTCC AATGTGCAGC GCTATGACCT GGCTGCCCAC 
CTAGGCCTGC GTGCCCCCAG CCCCCGGGAC AGCGACGCAG ACAGCGACCA GGGCAAGAAG 
GAGAGCGATG GGGAGGAAGG CCGCCAGCCC CAGCCCAAGC GGGAGGGCCC TATTGGCGGG 
GAGAGCGACT CAGAGGAGAT GTGCGAGAAG ACGTCGGGCT TCGGCAGCGG AAAGCCATCG 
GAGGCGAGCG AGCCATGTCC CGCCGAGCTC CTGCGGGCCA GGGAGGACAC CGAGTGCCTG 
GCGAGCATAA AGCACGAGGC TGAGCGCCTG GAGCGAACAG TGGAGCGCCT CATCACGGAC 
ACCGCGGGCT TCGGGGACGA TGTGGGGCTG CTGGGCAGTG GCGAGCCCTT GCCAGGGCCC 
GACATCCAGG GGGCGGGGGG GACGGGCGAG GGGGGCAAGA AGGAGCCCCC ACTGCTGGGG 
ACCATCAACT CGAGGATGAA GGCTTTCAGG AAGGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGAATCG GGGATGGCCT GCGGGAGCGC CTGGAAGGCC TGTCCCCCCT GCCTCACCTC 
ACAGAGTCTT CCAGCTTTCT CTCCACCGTC ACTTCCGTGT CCCGGGACTC CCCCGTCGGG 
AACCTGGGGA AGGAGCTGGT CCCGGACCTG CAGTCCAAAC TGAGAGGTCA GCTGGAGTGG 
CAGCCGGGGC AGGAGTGCGG GGACGAGCCA GAGCAGCTGC ACCGCCGCGC TGATGGGGAC 
GTCGGGAGCT GCCGGCAGGG AGGCCAGAGC TGCTTCAGTC TAGAGAGTGT ATCGCTAGAG 
GAGCTGCAGG GTCAGCTTGC GCAGGCGACC AGACTGCATC AAGAGGAGAC AGAGAAATTT 
ACAAACAAGA TTCGGAAGAT GGAGGAGGAG CACCTGTACG CCTTGAGGTG GAAAGAACTG 
GAAATGCACA GCCTGGCTTT GCAGAACACC CTCCATGAGC AAACCTGGAG TGATGAGAGG 
AACCTGATGC AGCAGGAGCT CCGGTCTATG AAGCAGAACA TTTTCCTTTT CTACGTCAAA 
CTCAGATGGC TGCTGAGGCA CTGGCGACAA GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT 
TTCACTGAGA GCGAGCATCC AGAGACCCTC CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG 
GACCCGAAGG GGGACGGCCC AGACCAAGAT GACAACGGTC CGGGCTGCGG CTTTCCGCTG 
GGAGAGCATC CTCCACACTC CCCTGTGCAG ATTGGTGACC ATGGATCGAG ACTACAGCCT 
ACACATGGGA GACAGCTCCA CAGGCAGGTG GTGGAAAACC AGCAGCTGTT CAGTGCACTC 
AAGAGCCTGC TGGAGGACCT GCGCGCCGAG CTGCGGGAGG ACGAGCATGC ACGGCGGCGG 
CTGCAGCAGC AGTACGCCAG CGACAAAGCC GCCTGGGACG TGGAGTGGGC TGCACTTACC 
TGCCGCCTGG AACAGGTGCC TGCCTGCGGG GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC 
TGCCATCTGG GCCAGCTGGA AGAGAAGACT GAGAAGAATT TGGGAGAAGC ATGCTCTTCC 
ACCGACAGCA AAGGGGCATT CAAGAAGGAA AGAGAGATGC ATCAGAAGCT CCTCACTGAC 
AGTCATGGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGTGAGAA GAACTGGAGC 
CGGGAGAAGG TGGAACTTCT TAGCCGTCTA GACAGAGACC GGCAGGAATG GGAGCAGCAG 
AAGAAGGAGC TCTTGTGGAG AATAGAACAG TTGCAGAAAG AAAACAGTCC CCGGAGAAGT 
GGCAGTTTCC TCTGTGATCA AAAAGAAGGC ACCATCCGCC CCTTTGCCCA TCCAGGAAGC 
CCCCGAATGC CCCGTCCCTT GGGGATGTGG TCCTGTGCAG ACACCGACTC CACCCCATTC 
GAAGACCGGC CGCTGTCCAA GCTGAAGGAA TCAGACAGAT GTTCAGCACG GGAAAACCTC 
TACTTGGACG CCTTGTCTCT AGATGACGAG CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG 
CGGGAGTTCA GGAACTGCCT CCCCCAGGAA GAAGAAAATC ACAAGGGAAA TCTTCAAAGG 
GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC CAGCGTCTGA TGGACGTTTC TCCCTTCCTG 
CCGGAGAAGG GCGTCCCCGC CACCAGCAGC AAGGAGGACA TCACTCCGCC CCTGTCTCCT 
GATGATCTGA AGTACATCGA GGAGTTCAAC AGCAAGAACT GGGACGAGAG GCCCCCGGAG 
CCCTGGGCAG ACAGGACCGA GGGGGGGCGG GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC 
CCTGACTCCT CCTGGTACCT CACCACGAGT GTCACCATGA CTACGGACAC CATGACCAGC 
CCAGAGCACT GCCAGAAGCA GCCCCTTCGG AGCCCCATGT GCGCGGAGCA GGCCGGGGTG 
CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT AGGATGGATG GCATCGTGGT GGCGGGCAGT 
GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC 
ACTGGAGACA CAAAAGGGGG TCCCTCAGAA CACGTGCTCA GCAGGTGGCC TTGCGCCCCC 
TCCAGGCACC CCCGAGACTA TGTGGAGGGA GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC 
GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC GGCCTGCAGA TGTCCAAGAA CATGAGTGAT 
GACATGAAGG AGGTGGCCTT CTCGGTCAGG AACGCCATCT GCTCTGGCCC CCCCAAGCCT 
CCGCTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGACGGGAAC ACAGACCGTC 
CAGACCATCA CCATTGGCCT GCAGACCGAA GCCCTGCGTG GCAGCGCCGT CACCAGCAGC 
CCCCACAAGT GTCTCACACC CAAGGCCGGG GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC 
CGTGGCCTTC GGAGCCGGCA GATGGCCCCC GCCATCGAGA AGGTGCAGGC CAAGTTCGAA 
CGCACGTGCT GCTCCCCCAA GTACGGGTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA 
GCTGAGCCGC CCAACAGCAG GACCTCCCTG GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC 
GCCTGGGCCC GTTCCACCAC CACGAGGGAG AGCCCGGTGC ACACCGCCAT CAACGATGGC 
CTCTCCAGCC TCTTCAACAT CATCGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG 
GGGACTCGGG CCGGGAGCCG ATCTCGCTCA GCAGAACCCC GGCCCGAGCT GGGCCCAGGC 
CAGGAAACGG GCCCCAGTTC CCGGGGCCGG TCCCCCAGCC CCATCGGGGC TGGCTCAGAG 
ACTTGGAGGG AGGAAGGGGG AGAGGGCACG CCCTTGAGGC AGGACTTATC CGCTCCCCCT 
GGTTACACGT TCACTGAGAA CGTAGCCCGG ATCCTCAACA AGAAGCTGTT GGAGCATGCC 
TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC GGGCCCCCAA GTCTTAACAG TGACAGCCAC 
ATGGGAGAAA CCATCAAAGC TGAACCAGGG TCCATCGAGG AACTACCTTG TTCCGCACTA 
GCTCCATCCC TAGAACCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCTTCCG 
TCCCGTTGGG CCTCACATTC CCCCACTGCC TCACTGGCTC AGTCACCCGG AGACCCAGCC 
TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA CCGGGGGAGA AGACACACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277327.1
CDS	117..5210
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQSGGTERLGNCRSTTKTQRKLAPRRKDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQVPACGVCPGSPQLLLCHLGQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421619.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421619.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
AGTGGTGGAA CCGAGCGCCT TGGAAACTGC AGGTCTACAA CCAAAACTCA AAGGAAACTG 
GCACCCCGGC GCAAGGATGA CAGTGCCGAT TTGAAGTGCC AGCTTCAGTT TGCCAAAGAG 
GAAGGCTCCC TGATGCGTAA AAAGATGGCC AAGCTGGGGA AGGAGAAGGA CGAGCTGGAA 
CAGGAGCTCC AGAAGTACAA GGCCCTCTAT GGAGACGTGG ACAGCCCCCT CCCCACGGGG 
GAAGCCGGGG GGCCGCCCAG CACCAGGGAG GCTGAGCTGA AGCTGCGCTT GAAGTTGGTG 
GAGGAAGAGG CCAACATCTT GGGCCGGAAG ATTGTGGAAC TGGAGGTGGA GAACCGAGGC 
CTCAAAGCAG AAATGGAGGA CATGAGGCTC CAGTACGAGC GAGAGGGTAC AGGCCGGGAC 
CACATCACAG GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC GACGGAGCTC 
CGCCGGCACC TGCAGTTCGT GGAGGAGGAA GCGGAGCTGC TCCGGAGGTC CATATCTGAG 
ATCGAAGACC ACAACCGGCA GCTGACCCAT GAGCTGAACA AGTTCAAGTT TGAGCCTCGG 
CCGGAGCCAG GGTGGCTGGC GGATGGCGCG GGCAAGGGCG TGGGCAGCGG GGCCCCTCTG 
CAGGAGGAGC TCAAGTCGGC ACGGCTACAG ATCAATGAGC TCAGTGGGAA GGTGCTGAAG 
CTGCAGTATG AGAACCGGGT GCTACTGTCC AATGTGCAGC GCTATGACCT GGCTGCCCAC 
CTAGGCCTGC GTGCCCCCAG CCCCCGGGAC AGCGACGCAG ACAGCGACCA GGGCAAGAAG 
GAGAGCGATG GGGAGGAAGG CCGCCAGCCC CAGCCCAAGC GGGAGGGCCC TATTGGCGGG 
GAGAGCGACT CAGAGGAGAT GTGCGAGAAG ACGTCGGGCT TCGGCAGCGG AAAGCCATCG 
GAGGCGAGCG AGCCATGTCC CGCCGAGCTC CTGCGGGCCA GGGAGGACAC CGAGTGCCTG 
GCGAGCATAA AGCACGAGGC TGAGCGCCTG GAGCGAACAG TGGAGCGCCT CATCACGGAC 
ACCGCGGGCT TCGGGGACGA TGTGGGGCTG CTGGGCAGTG GCGAGCCCTT GCCAGGGCCC 
GACATCCAGG GGGCGGGGGG GACGGGCGAG GGGGGCAAGA AGGAGCCCCC ACTGCTGGGG 
ACCATCAACT CGAGGATGAA GGCTTTCAGG AAGGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGAATCG GGGATGGCCT GCGGGAGCGC CTGGAAGGCC TGTCCCCCCT GCCTCACCTC 
ACAGAGTCTT CCAGCTTTCT CTCCACCGTC ACTTCCGTGT CCCGGGACTC CCCCGTCGGG 
AACCTGGGGA AGGAGCTGGT CCCGGACCTG CAGTCCAAAC TGAGAGGTCA GCTGGAGTGG 
CAGCCGGGGC AGGAGTGCGG GGACGAGCCA GAGCAGCTGC ACCGCCGCGC TGATGGGGAC 
GTCGGGAGCT GCCGGCAGGG AGGCCAGAGC TGCTTCAGTC TAGAGAGTGT ATCGCTAGAG 
GAGCTGCAGG GTCAGCTTGC GCAGGCGACC AGACTGCATC AAGAGGAGAC AGAGAAATTT 
ACAAACAAGA TTCGGAAGAT GGAGGAGGAG CACCTGTACG CCTTGAGGTG GAAAGAACTG 
GAAATGCACA GCCTGGCTTT GCAGAACACC CTCCATGAGC AAACCTGGAG TGATGAGAGG 
AACCTGATGC AGCAGGAGCT CCGGTCTATG AAGCAGAACA TTTTCCTTTT CTACGTCAAA 
CTCAGATGGC TGCTGAGGCA CTGGCGACAA GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT 
TTCACTGAGA GCGAGCATCC AGAGACCCTC CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG 
GACCCGAAGG GGGACGGCCC AGACCAAGAT GACAACGGTC CGGGCTGCGG CTTTCCGCTG 
GGAGAGCATC CTCCACACTC CCCTGTGCAG ATTGGTGACC ATGGATCGAG ACTACAGCCT 
ACACATGGGA GACAGCTCCA CAGGCAGGTG GTGGAAAACC AGCAGCTGTT CAGTGCACTC 
AAGAGCCTGC TGGAGGACCT GCGCGCCGAG CTGCGGGAGG ACGAGCATGC ACGGCGGCGG 
CTGCAGCAGC AGTACGCCAG CGACAAAGCC GCCTGGGACG TGGAGTGGGC TGCACTTACC 
TGCCGCCTGG AACAGGTGCC TGCCTGCGGG GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC 
TGCCATCTGG GCCAGCTGGA AGAGAAGACT GAGAAGAATT TGGGAGAAGC ATGCTCTTCC 
ACCGACAGCA AAGGGGCATT CAAGAAGGAA AGAGAGATGC ATCAGAAGCT CCTCACTGAC 
AGTCATGGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGTGAGAA GAACTGGAGC 
CGGGAGAAGG TGGAACTTCT TAGCCGTCTA GACAGAGACC GGCAGGAATG GGAGCAGCAG 
AAGAAGGAGC TCTTGTGGAG AATAGAACAG TTGCAGAAAG AAAACAGTCC CCGGAGAAGT 
GGCAGTTTCC TCTGTGATCA AAAAGAAGGC ACCATCCGCC CCTTTGCCCA TCCAGGAAGC 
CCCCGAATGC CCCGTCCCTT GGGGATGTGG TCCTGTGCAG ACACCGACTC CACCCCATTC 
GAAGACCGGC CGCTGTCCAA GCTGAAGGAA TCAGACAGAT GTTCAGCACG GGAAAACCTC 
TACTTGGACG CCTTGTCTCT AGATGACGAG CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG 
CGGGAGTTCA GGAACTGCCT CCCCCAGGAA GAAGAAAATC ACAAGGGAAA TCTTCAAAGG 
GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC CAGCGTCTGA TGGACGTTTC TCCCTTCCTG 
CCGGAGAAGG GCGTCCCCGC CACCAGCAGC AAGGAGGACA TCACTCCGCC CCTGTCTCCT 
GATGATCTGA AGTACATCGA GGAGTTCAAC AGCAAGAACT GGGACGAGAG GCCCCCGGAG 
CCCTGGGCAG ACAGGACCGA GGGGGGGCGG GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC 
CCTGACTCCT CCTGGTACCT CACCACGAGT GTCACCATGA CTACGGACAC CATGACCAGC 
CCAGAGCACT GCCAGAAGCA GCCCCTTCGG AGCCCCATGT GCGCGGAGCA GGCCGGGGTG 
CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT AGGATGGATG GCATCGTGGT GGCGGGCAGT 
GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC 
ACTGGAGACA CAAAAGGGGG TCCCTCAGAA CACGTGCTCA GCAGGTGGCC TTGCGCCCCC 
TCCAGGCACC CCCGAGACTA TGTGGAGGGA GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC 
GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC GGCCTGCAGA TGTCCAAGAA CATGAGTGAT 
GACATGAAGG AGGTGGCCTT CTCGGTCAGG AACGCCATCT GCTCTGGCCC CCCCAAGCCT 
CCGCTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGACGGGAAC ACAGACCGTC 
CAGACCATCA CCATTGGCCT GCAGACCGAA GCCCTGCGTG GCAGCGCCGT CACCAGCAGC 
CCCCACAAGT GTCTCACACC CAAGGCCGGG GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC 
CGTGGCCTTC GGAGCCGGCA GATGGCCCCC GCCATCGAGA AGGTGCAGGC CAAGTTCGAA 
CGCACGTGCT GCTCCCCCAA GTACGGGTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA 
GCTGAGCCGC CCAACAGCAG GACCTCCCTG GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC 
GCCTGGGCCC GTTCCACCAC CACGAGGGAG AGCCCGGTGC ACACCGCCAT CAACGATGGC 
CTCTCCAGCC TCTTCAACAT CATCGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG 
GGGACTCGGG CCGGGAGCCG ATCTCGCTCA GCAGAACCCC GGCCCGAGCT GGGCCCAGGC 
CAGGAAACGG GCCCCAGTTC CCGGGGCCGG TCCCCCAGCC CCATCGGGGC TGGCTCAGAG 
ACTTGGAGGG AGGAAGGGGG AGAGGGCACG CCCTTGAGGC AGGACTTATC CGCTCCCCCT 
GGTTACACGT TCACTGAGAA CGTAGCCCGG ATCCTCAACA AGAAGCTGTT GGAGCATGCC 
TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC GGGCCCCCAA GTCTTAACAG TGACAGCCAC 
ATGGGAGAAA CCATCAAAGC TGAACCAGGG TCCATCGAGG AACTACCTTG TTCCGCACTA 
GCTCCATCCC TAGAACCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCTTCCG 
TCCCGTTGGG CCTCACATTC CCCCACTGCC TCACTGGCTC AGTCACCCGG AGACCCAGCC 
TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA CCGGGGGAGA AGACACACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421621.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277329.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe289880
Clone ID Related Accession (Same CDS sequence)	XM_022421621.1
Accession Version	XM_022421621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA TGAGAGACAG TTACTTAGAG GAAGATGTTT ACCAGCTGCA GGAGCTTCGG 
CGAGAGCTGG ACCGTGCTCA TAAAAACTGC CGAATCCTGC AGTACCGCCT CAGGAAAGCT 
GAGCAGAAGA GCCTGAAAGT GGCTGAGACT GGTCAGGTGG ATGGTGAGCT CATCCGGAGT 
CTAGAGCAGG ATTTAAAGGT AGCCAAAGAT GTATCTGTCA GATTGCATCA TGAACTTGAA 
ACCGTGGAGG AAAAGCGTGC TAAAGCAGAG GATGAAAATG AAAGTCTCCG GCAGCAGATG 
ATTGAAGTGG AGATATCTAA ACAGGCCCTC CAAAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTGAAAA GAAGAGGCAA CCGAGAAACA TACAAAGAAA AGAAAACCTT TAACCAGCAG 
AGTGGTGGAA CCGAGCGCCT TGGAAACTGC AGGTCTACAA CCAAAACTCA AAGGAAACTG 
GCACCCCGGC GCAAGGATGA CAGTGCCGAT TTGAAGTGCC AGCTTCAGTT TGCCAAAGAG 
GAAGGCTCCC TGATGCGTAA AAAGATGGCC AAGCTGGGGA AGGAGAAGGA CGAGCTGGAA 
CAGGAGCTCC AGAAGTACAA GGCCCTCTAT GGAGACGTGG ACAGCCCCCT CCCCACGGGG 
GAAGCCGGGG GGCCGCCCAG CACCAGGGAG GCTGAGCTGA AGCTGCGCTT GAAGTTGGTG 
GAGGAAGAGG CCAACATCTT GGGCCGGAAG ATTGTGGAAC TGGAGGTGGA GAACCGAGGC 
CTCAAAGCAG AAATGGAGGA CATGAGGCTC CAGTACGAGC GAGAGGGTAC AGGCCGGGAC 
CACATCACAG GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC GACGGAGCTC 
CGCCGGCACC TGCAGTTCGT GGAGGAGGAA GCGGAGCTGC TCCGGAGGTC CATATCTGAG 
ATCGAAGACC ACAACCGGCA GCTGACCCAT GAGCTGAACA AGTTCAAGTT TGAGCCTCGG 
CCGGAGCCAG GGTGGCTGGC GGATGGCGCG GGCAAGGGCG TGGGCAGCGG GGCCCCTCTG 
CAGGAGGAGC TCAAGTCGGC ACGGCTACAG ATCAATGAGC TCAGTGGGAA GGTGCTGAAG 
CTGCAGTATG AGAACCGGGT GCTACTGTCC AATGTGCAGC GCTATGACCT GGCTGCCCAC 
CTAGGCCTGC GTGCCCCCAG CCCCCGGGAC AGCGACGCAG ACAGCGACCA GGGCAAGAAG 
GAGAGCGATG GGGAGGAAGG CCGCCAGCCC CAGCCCAAGC GGGAGGGCCC TATTGGCGGG 
GAGAGCGACT CAGAGGAGAT GTGCGAGAAG ACGTCGGGCT TCGGCAGCGG AAAGCCATCG 
GAGGCGAGCG AGCCATGTCC CGCCGAGCTC CTGCGGGCCA GGGAGGACAC CGAGTGCCTG 
GCGAGCATAA AGCACGAGGC TGAGCGCCTG GAGCGAACAG TGGAGCGCCT CATCACGGAC 
ACCGCGGGCT TCGGGGACGA TGTGGGGCTG CTGGGCAGTG GCGAGCCCTT GCCAGGGCCC 
GACATCCAGG GGGCGGGGGG GACGGGCGAG GGGGGCAAGA AGGAGCCCCC ACTGCTGGGG 
ACCATCAACT CGAGGATGAA GGCTTTCAGG AAGGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGAATCG GGGATGGCCT GCGGGAGCGC CTGGAAGGCC TGTCCCCCCT GCCTCACCTC 
ACAGAGTCTT CCAGCTTTCT CTCCACCGTC ACTTCCGTGT CCCGGGACTC CCCCGTCGGG 
AACCTGGGGA AGGAGCTGGT CCCGGACCTG CAGTCCAAAC TGAGAGGTCA GCTGGAGTGG 
CAGCCGGGGC AGGAGTGCGG GGACGAGCCA GAGCAGCTGC ACCGCCGCGC TGATGGGGAC 
GTCGGGAGCT GCCGGCAGGG AGGCCAGAGC TGCTTCAGTC TAGAGAGTGT ATCGCTAGAG 
GAGCTGCAGG GTCAGCTTGC GCAGGCGACC AGACTGCATC AAGAGGAGAC AGAGAAATTT 
ACAAACAAGA TTCGGAAGAT GGAGGAGGAG CACCTGTACG CCTTGAGGTG GAAAGAACTG 
GAAATGCACA GCCTGGCTTT GCAGAACACC CTCCATGAGC AAACCTGGAG TGATGAGAGG 
AACCTGATGC AGCAGGAGCT CCGGTCTATG AAGCAGAACA TTTTCCTTTT CTACGTCAAA 
CTCAGATGGC TGCTGAGGCA CTGGCGACAA GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT 
TTCACTGAGA GCGAGCATCC AGAGACCCTC CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG 
GACCCGAAGG GGGACGGCCC AGACCAAGAT GACAACGGTC CGGGCTGCGG CTTTCCGCTG 
GGAGAGCATC CTCCACACTC CCCTGTGCAG ATTGGTGACC ATGGATCGAG ACTACAGCCT 
ACACATGGGA GACAGCTCCA CAGGCAGGTG GTGGAAAACC AGCAGCTGTT CAGTGCACTC 
AAGAGCCTGC TGGAGGACCT GCGCGCCGAG CTGCGGGAGG ACGAGCATGC ACGGCGGCGG 
CTGCAGCAGC AGTACGCCAG CGACAAAGCC GCCTGGGACG TGGAGTGGGC TGCACTTACC 
TGCCGCCTGG AACAGGTGCC TGCCTGCGGG GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC 
TGCCATCTGG GCCAGCTGGA AGAGAAGACT GAGAAGAATT TGGGAGAAGC ATGCTCTTCC 
ACCGACAGCA AAGGGGCATT CAAGAAGGAA AGAGAGATGC ATCAGAAGCT CCTCACTGAC 
AGTCATGGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGTGAGAA GAACTGGAGC 
CGGGAGAAGG TGGAACTTCT TAGCCGTCTA GACAGAGACC GGCAGGAATG GGAGCAGCAG 
AAGAAGGAGC TCTTGTGGAG AATAGAACAG TTGCAGAAAG AAAACAGTCC CCGGAGAAGT 
GGCAGTTTCC TCTGTGATCA AAAAGAAGGC ACCATCCGCC CCTTTGCCCA TCCAGGAAGC 
CCCCGAATGC CCCGTCCCTT GGGGATGTGG TCCTGTGCAG ACACCGACTC CACCCCATTC 
GAAGACCGGC CGCTGTCCAA GCTGAAGGAA TCAGACAGAT GTTCAGCACG GGAAAACCTC 
TACTTGGACG CCTTGTCTCT AGATGACGAG CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG 
CGGGAGTTCA GGAACTGCCT CCCCCAGGAA GAAGAAAATC ACAAGGGAAA TCTTCAAAGG 
GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC CAGCGTCTGA TGGACGTTTC TCCCTTCCTG 
CCGGAGAAGG GCGTCCCCGC CACCAGCAGC AAGGAGGACA TCACTCCGCC CCTGTCTCCT 
GATGATCTGA AGTACATCGA GGAGTTCAAC AGCAAGAACT GGGACGAGAG GCCCCCGGAG 
CCCTGGGCAG ACAGGACCGA GGGGGGGCGG GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC 
CCTGACTCCT CCTGGTACCT CACCACGAGT GTCACCATGA CTACGGACAC CATGACCAGC 
CCAGAGCACT GCCAGAAGCA GCCCCTTCGG AGCCCCATGT GCGCGGAGCA GGCCGGGGTG 
CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT AGGATGGATG GCATCGTGGT GGCGGGCAGT 
GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC 
ACTGGAGACA CAAAAGGGGG TCCCTCAGAA CACGTGCTCA GCAGGTGGCC TTGCGCCCCC 
TCCAGGCACC CCCGAGACTA TGTGGAGGGA GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC 
GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC GGCCTGCAGA TGTCCAAGAA CATGAGTGAT 
GACATGAAGG AGGTGGCCTT CTCGGTCAGG AACGCCATCT GCTCTGGCCC CCCCAAGCCT 
CCGCTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGACGGGAAC ACAGACCGTC 
CAGACCATCA CCATTGGCCT GCAGACCGAA GCCCTGCGTG GCAGCGCCGT CACCAGCAGC 
CCCCACAAGT GTCTCACACC CAAGGCCGGG GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC 
CGTGGCCTTC GGAGCCGGCA GATGGCCCCC GCCATCGAGA AGGTGCAGGC CAAGTTCGAA 
CGCACGTGCT GCTCCCCCAA GTACGGGTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA 
GCTGAGCCGC CCAACAGCAG GACCTCCCTG GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC 
GCCTGGGCCC GTTCCACCAC CACGAGGGAG AGCCCGGTGC ACACCGCCAT CAACGATGGC 
CTCTCCAGCC TCTTCAACAT CATCGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG 
GGGACTCGGG CCGGGAGCCG ATCTCGCTCA GCAGAACCCC GGCCCGAGCT GGGCCCAGGC 
CAGGAAACGG GCCCCAGTTC CCGGGGCCGG TCCCCCAGCC CCATCGGGGC TGGCTCAGAG 
ACTTGGAGGG AGGAAGGGGG AGAGGGCACG CCCTTGAGGC AGGACTTATC CGCTCCCCCT 
GGTTACACGT TCACTGAGAA CGTAGCCCGG ATCCTCAACA AGAAGCTGTT GGAGCATGCC 
TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC GGGCCCCCAA GTCTTAACAG TGACAGCCAC 
ATGGGAGAAA CCATCAAAGC TGAACCAGGG TCCATCGAGG AACTACCTTG TTCCGCACTA 
GCTCCATCCC TAGAACCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCTTCCG 
TCCCGTTGGG CCTCACATTC CCCCACTGCC TCACTGGCTC AGTCACCCGG AGACCCAGCC 
TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA CCGGGGGAGA AGACACACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277329.1
CDS	1029..6062
Translation	MEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQQSGGTERLGNCRSTTKTQRKLAPRRKDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQVPACGVCPGSPQLLLCHLGQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421621.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421621.1

ORF Insert Sequence:

ATGGAAGAGA TGAGAGACAG TTACTTAGAG GAAGATGTTT ACCAGCTGCA GGAGCTTCGG 
CGAGAGCTGG ACCGTGCTCA TAAAAACTGC CGAATCCTGC AGTACCGCCT CAGGAAAGCT 
GAGCAGAAGA GCCTGAAAGT GGCTGAGACT GGTCAGGTGG ATGGTGAGCT CATCCGGAGT 
CTAGAGCAGG ATTTAAAGGT AGCCAAAGAT GTATCTGTCA GATTGCATCA TGAACTTGAA 
ACCGTGGAGG AAAAGCGTGC TAAAGCAGAG GATGAAAATG AAAGTCTCCG GCAGCAGATG 
ATTGAAGTGG AGATATCTAA ACAGGCCCTC CAAAATGAGC TGGAGAGACT GAAAGAGAGT 
TCCCTGAAAA GAAGAGGCAA CCGAGAAACA TACAAAGAAA AGAAAACCTT TAACCAGCAG 
AGTGGTGGAA CCGAGCGCCT TGGAAACTGC AGGTCTACAA CCAAAACTCA AAGGAAACTG 
GCACCCCGGC GCAAGGATGA CAGTGCCGAT TTGAAGTGCC AGCTTCAGTT TGCCAAAGAG 
GAAGGCTCCC TGATGCGTAA AAAGATGGCC AAGCTGGGGA AGGAGAAGGA CGAGCTGGAA 
CAGGAGCTCC AGAAGTACAA GGCCCTCTAT GGAGACGTGG ACAGCCCCCT CCCCACGGGG 
GAAGCCGGGG GGCCGCCCAG CACCAGGGAG GCTGAGCTGA AGCTGCGCTT GAAGTTGGTG 
GAGGAAGAGG CCAACATCTT GGGCCGGAAG ATTGTGGAAC TGGAGGTGGA GAACCGAGGC 
CTCAAAGCAG AAATGGAGGA CATGAGGCTC CAGTACGAGC GAGAGGGTAC AGGCCGGGAC 
CACATCACAG GCATTCCTAC CTCACCCTTC GGTGACTCCC TGGAGTCCTC GACGGAGCTC 
CGCCGGCACC TGCAGTTCGT GGAGGAGGAA GCGGAGCTGC TCCGGAGGTC CATATCTGAG 
ATCGAAGACC ACAACCGGCA GCTGACCCAT GAGCTGAACA AGTTCAAGTT TGAGCCTCGG 
CCGGAGCCAG GGTGGCTGGC GGATGGCGCG GGCAAGGGCG TGGGCAGCGG GGCCCCTCTG 
CAGGAGGAGC TCAAGTCGGC ACGGCTACAG ATCAATGAGC TCAGTGGGAA GGTGCTGAAG 
CTGCAGTATG AGAACCGGGT GCTACTGTCC AATGTGCAGC GCTATGACCT GGCTGCCCAC 
CTAGGCCTGC GTGCCCCCAG CCCCCGGGAC AGCGACGCAG ACAGCGACCA GGGCAAGAAG 
GAGAGCGATG GGGAGGAAGG CCGCCAGCCC CAGCCCAAGC GGGAGGGCCC TATTGGCGGG 
GAGAGCGACT CAGAGGAGAT GTGCGAGAAG ACGTCGGGCT TCGGCAGCGG AAAGCCATCG 
GAGGCGAGCG AGCCATGTCC CGCCGAGCTC CTGCGGGCCA GGGAGGACAC CGAGTGCCTG 
GCGAGCATAA AGCACGAGGC TGAGCGCCTG GAGCGAACAG TGGAGCGCCT CATCACGGAC 
ACCGCGGGCT TCGGGGACGA TGTGGGGCTG CTGGGCAGTG GCGAGCCCTT GCCAGGGCCC 
GACATCCAGG GGGCGGGGGG GACGGGCGAG GGGGGCAAGA AGGAGCCCCC ACTGCTGGGG 
ACCATCAACT CGAGGATGAA GGCTTTCAGG AAGGAGCTGC AGGCCTTCCT GGAGCAGGTG 
AACCGAATCG GGGATGGCCT GCGGGAGCGC CTGGAAGGCC TGTCCCCCCT GCCTCACCTC 
ACAGAGTCTT CCAGCTTTCT CTCCACCGTC ACTTCCGTGT CCCGGGACTC CCCCGTCGGG 
AACCTGGGGA AGGAGCTGGT CCCGGACCTG CAGTCCAAAC TGAGAGGTCA GCTGGAGTGG 
CAGCCGGGGC AGGAGTGCGG GGACGAGCCA GAGCAGCTGC ACCGCCGCGC TGATGGGGAC 
GTCGGGAGCT GCCGGCAGGG AGGCCAGAGC TGCTTCAGTC TAGAGAGTGT ATCGCTAGAG 
GAGCTGCAGG GTCAGCTTGC GCAGGCGACC AGACTGCATC AAGAGGAGAC AGAGAAATTT 
ACAAACAAGA TTCGGAAGAT GGAGGAGGAG CACCTGTACG CCTTGAGGTG GAAAGAACTG 
GAAATGCACA GCCTGGCTTT GCAGAACACC CTCCATGAGC AAACCTGGAG TGATGAGAGG 
AACCTGATGC AGCAGGAGCT CCGGTCTATG AAGCAGAACA TTTTCCTTTT CTACGTCAAA 
CTCAGATGGC TGCTGAGGCA CTGGCGACAA GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT 
TTCACTGAGA GCGAGCATCC AGAGACCCTC CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG 
GACCCGAAGG GGGACGGCCC AGACCAAGAT GACAACGGTC CGGGCTGCGG CTTTCCGCTG 
GGAGAGCATC CTCCACACTC CCCTGTGCAG ATTGGTGACC ATGGATCGAG ACTACAGCCT 
ACACATGGGA GACAGCTCCA CAGGCAGGTG GTGGAAAACC AGCAGCTGTT CAGTGCACTC 
AAGAGCCTGC TGGAGGACCT GCGCGCCGAG CTGCGGGAGG ACGAGCATGC ACGGCGGCGG 
CTGCAGCAGC AGTACGCCAG CGACAAAGCC GCCTGGGACG TGGAGTGGGC TGCACTTACC 
TGCCGCCTGG AACAGGTGCC TGCCTGCGGG GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC 
TGCCATCTGG GCCAGCTGGA AGAGAAGACT GAGAAGAATT TGGGAGAAGC ATGCTCTTCC 
ACCGACAGCA AAGGGGCATT CAAGAAGGAA AGAGAGATGC ATCAGAAGCT CCTCACTGAC 
AGTCATGGCC TGGTCATGGA CCTGCGCTGG CAGATCCATC ACAGTGAGAA GAACTGGAGC 
CGGGAGAAGG TGGAACTTCT TAGCCGTCTA GACAGAGACC GGCAGGAATG GGAGCAGCAG 
AAGAAGGAGC TCTTGTGGAG AATAGAACAG TTGCAGAAAG AAAACAGTCC CCGGAGAAGT 
GGCAGTTTCC TCTGTGATCA AAAAGAAGGC ACCATCCGCC CCTTTGCCCA TCCAGGAAGC 
CCCCGAATGC CCCGTCCCTT GGGGATGTGG TCCTGTGCAG ACACCGACTC CACCCCATTC 
GAAGACCGGC CGCTGTCCAA GCTGAAGGAA TCAGACAGAT GTTCAGCACG GGAAAACCTC 
TACTTGGACG CCTTGTCTCT AGATGACGAG CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG 
CGGGAGTTCA GGAACTGCCT CCCCCAGGAA GAAGAAAATC ACAAGGGAAA TCTTCAAAGG 
GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC CAGCGTCTGA TGGACGTTTC TCCCTTCCTG 
CCGGAGAAGG GCGTCCCCGC CACCAGCAGC AAGGAGGACA TCACTCCGCC CCTGTCTCCT 
GATGATCTGA AGTACATCGA GGAGTTCAAC AGCAAGAACT GGGACGAGAG GCCCCCGGAG 
CCCTGGGCAG ACAGGACCGA GGGGGGGCGG GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC 
CCTGACTCCT CCTGGTACCT CACCACGAGT GTCACCATGA CTACGGACAC CATGACCAGC 
CCAGAGCACT GCCAGAAGCA GCCCCTTCGG AGCCCCATGT GCGCGGAGCA GGCCGGGGTG 
CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT AGGATGGATG GCATCGTGGT GGCGGGCAGT 
GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC 
ACTGGAGACA CAAAAGGGGG TCCCTCAGAA CACGTGCTCA GCAGGTGGCC TTGCGCCCCC 
TCCAGGCACC CCCGAGACTA TGTGGAGGGA GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC 
GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC GGCCTGCAGA TGTCCAAGAA CATGAGTGAT 
GACATGAAGG AGGTGGCCTT CTCGGTCAGG AACGCCATCT GCTCTGGCCC CCCCAAGCCT 
CCGCTCAAGG ACATGGCCTG CCAGACCAAT GGGTCCCGGA CGACGGGAAC ACAGACCGTC 
CAGACCATCA CCATTGGCCT GCAGACCGAA GCCCTGCGTG GCAGCGCCGT CACCAGCAGC 
CCCCACAAGT GTCTCACACC CAAGGCCGGG GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC 
CGTGGCCTTC GGAGCCGGCA GATGGCCCCC GCCATCGAGA AGGTGCAGGC CAAGTTCGAA 
CGCACGTGCT GCTCCCCCAA GTACGGGTCT CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA 
GCTGAGCCGC CCAACAGCAG GACCTCCCTG GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC 
GCCTGGGCCC GTTCCACCAC CACGAGGGAG AGCCCGGTGC ACACCGCCAT CAACGATGGC 
CTCTCCAGCC TCTTCAACAT CATCGACCAC AGCCCCGTGG TGCAGGACCC CTTCCAGAAG 
GGGACTCGGG CCGGGAGCCG ATCTCGCTCA GCAGAACCCC GGCCCGAGCT GGGCCCAGGC 
CAGGAAACGG GCCCCAGTTC CCGGGGCCGG TCCCCCAGCC CCATCGGGGC TGGCTCAGAG 
ACTTGGAGGG AGGAAGGGGG AGAGGGCACG CCCTTGAGGC AGGACTTATC CGCTCCCCCT 
GGTTACACGT TCACTGAGAA CGTAGCCCGG ATCCTCAACA AGAAGCTGTT GGAGCATGCC 
TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC GGGCCCCCAA GTCTTAACAG TGACAGCCAC 
ATGGGAGAAA CCATCAAAGC TGAACCAGGG TCCATCGAGG AACTACCTTG TTCCGCACTA 
GCTCCATCCC TAGAACCCTG CTTCTCCAGG CCCGAGAGAC CAGCAAACCG TCGCCTTCCG 
TCCCGTTGGG CCTCACATTC CCCCACTGCC TCACTGGCTC AGTCACCCGG AGACCCAGCC 
TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA CCGGGGGAGA AGACACACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421622.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277330.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.

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CloneID	OCe289881
Clone ID Related Accession (Same CDS sequence)	XM_022421622.1
Accession Version	XM_022421622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG AGTGTATCGC TAGAGGAGCT GCAGGGTCAG 
CTTGCGCAGG CGACCAGACT GCATCAAGAG GAGACAGAGA AATTTACAAA CAAGATTCGG 
AAGATGGAGG AGGAGCACCT GTACGCCTTG AGGTGGAAAG AACTGGAAAT GCACAGCCTG 
GCTTTGCAGA ACACCCTCCA TGAGCAAACC TGGAGTGATG AGAGGAACCT GATGCAGCAG 
GAGCTCCGGT CTATGAAGCA GAACATTTTC CTTTTCTACG TCAAACTCAG ATGGCTGCTG 
AGGCACTGGC GACAAGGGAA GCAGATGGAG GAAGAAGGCG AGGATTTCAC TGAGAGCGAG 
CATCCAGAGA CCCTCCCCAG GCTTGGTGAG CTTGGAGTGC AGGGGGACCC GAAGGGGGAC 
GGCCCAGACC AAGATGACAA CGGTCCGGGC TGCGGCTTTC CGCTGGGAGA GCATCCTCCA 
CACTCCCCTG TGCAGATTGG TGACCATGGA TCGAGACTAC AGCCTACACA TGGGAGACAG 
CTCCACAGGC AGGTGGTGGA AAACCAGCAG CTGTTCAGTG CACTCAAGAG CCTGCTGGAG 
GACCTGCGCG CCGAGCTGCG GGAGGACGAG CATGCACGGC GGCGGCTGCA GCAGCAGTAC 
GCCAGCGACA AAGCCGCCTG GGACGTGGAG TGGGCTGCAC TTACCTGCCG CCTGGAACAG 
GTGCCTGCCT GCGGGGTCTG TCCTGGGTCT CCCCAGCTCC TCCTCTGCCA TCTGGGCCAG 
CTGGAAGAGA AGACTGAGAA GAATTTGGGA GAAGCATGCT CTTCCACCGA CAGCAAAGGG 
GCATTCAAGA AGGAAAGAGA GATGCATCAG AAGCTCCTCA CTGACAGTCA TGGCCTGGTC 
ATGGACCTGC GCTGGCAGAT CCATCACAGT GAGAAGAACT GGAGCCGGGA GAAGGTGGAA 
CTTCTTAGCC GTCTAGACAG AGACCGGCAG GAATGGGAGC AGCAGAAGAA GGAGCTCTTG 
TGGAGAATAG AACAGTTGCA GAAAGAAAAC AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT 
GATCAAAAAG AAGGCACCAT CCGCCCCTTT GCCCATCCAG GAAGCCCCCG AATGCCCCGT 
CCCTTGGGGA TGTGGTCCTG TGCAGACACC GACTCCACCC CATTCGAAGA CCGGCCGCTG 
TCCAAGCTGA AGGAATCAGA CAGATGTTCA GCACGGGAAA ACCTCTACTT GGACGCCTTG 
TCTCTAGATG ACGAGCCAGA GGAGCCTCCA GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC 
TGCCTCCCCC AGGAAGAAGA AAATCACAAG GGAAATCTTC AAAGGGCCGT GTCTGTGTCC 
TCCATGTCTG AGTTCCAGCG TCTGATGGAC GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC 
CCCGCCACCA GCAGCAAGGA GGACATCACT CCGCCCCTGT CTCCTGATGA TCTGAAGTAC 
ATCGAGGAGT TCAACAGCAA GAACTGGGAC GAGAGGCCCC CGGAGCCCTG GGCAGACAGG 
ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG 
TACCTCACCA CGAGTGTCAC CATGACTACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCCC TTCGGAGCCC CATGTGCGCG GAGCAGGCCG GGGTGCGGGT GCTGCACAGC 
CCGCCCGCGG TCCGTAGGAT GGATGGCATC GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG 
GCCGAGCCCG AGTGCCCGTT TCCAACTAGC AGAGCCAGGG GGGGCACTGG AGACACAAAA 
GGGGGTCCCT CAGAACACGT GCTCAGCAGG TGGCCTTGCG CCCCCTCCAG GCACCCCCGA 
GACTATGTGG AGGGAGGGCT GCGCCCCCTC GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT 
GCCTCCTCGC TGCACGGCCT GCAGATGTCC AAGAACATGA GTGATGACAT GAAGGAGGTG 
GCCTTCTCGG TCAGGAACGC CATCTGCTCT GGCCCCCCCA AGCCTCCGCT CAAGGACATG 
GCCTGCCAGA CCAATGGGTC CCGGACGACG GGAACACAGA CCGTCCAGAC CATCACCATT 
GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC GCCGTCACCA GCAGCCCCCA CAAGTGTCTC 
ACACCCAAGG CCGGGGGCGG GGCCACGCCC GCGTCATCTC CTTCCCGTGG CCTTCGGAGC 
CGGCAGATGG CCCCCGCCAT CGAGAAGGTG CAGGCCAAGT TCGAACGCAC GTGCTGCTCC 
CCCAAGTACG GGTCTCCCAA GCTGCAGAGG AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC 
AGCAGGACCT CCCTGGGGCT GGCCCCAAAG GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC 
ACCACCACGA GGGAGAGCCC GGTGCACACC GCCATCAACG ATGGCCTCTC CAGCCTCTTC 
AACATCATCG ACCACAGCCC CGTGGTGCAG GACCCCTTCC AGAAGGGGAC TCGGGCCGGG 
AGCCGATCTC GCTCAGCAGA ACCCCGGCCC GAGCTGGGCC CAGGCCAGGA AACGGGCCCC 
AGTTCCCGGG GCCGGTCCCC CAGCCCCATC GGGGCTGGCT CAGAGACTTG GAGGGAGGAA 
GGGGGAGAGG GCACGCCCTT GAGGCAGGAC TTATCCGCTC CCCCTGGTTA CACGTTCACT 
GAGAACGTAG CCCGGATCCT CAACAAGAAG CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG 
AGGCAGGCCC CCCACGGGCC CCCAAGTCTT AACAGTGACA GCCACATGGG AGAAACCATC 
AAAGCTGAAC CAGGGTCCAT CGAGGAACTA CCTTGTTCCG CACTAGCTCC ATCCCTAGAA 
CCCTGCTTCT CCAGGCCCGA GAGACCAGCA AACCGTCGCC TTCCGTCCCG TTGGGCCTCA 
CATTCCCCCA CTGCCTCACT GGCTCAGTCA CCCGGAGACC CAGCCTCCTT GGAGGAGCTC 
GGCGATGAGG AGCCACCGGG GGAGAAGACA CACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277330.1
CDS	117..5135
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQVPACGVCPGSPQLLLCHLGQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421622.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421622.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG AGTGTATCGC TAGAGGAGCT GCAGGGTCAG 
CTTGCGCAGG CGACCAGACT GCATCAAGAG GAGACAGAGA AATTTACAAA CAAGATTCGG 
AAGATGGAGG AGGAGCACCT GTACGCCTTG AGGTGGAAAG AACTGGAAAT GCACAGCCTG 
GCTTTGCAGA ACACCCTCCA TGAGCAAACC TGGAGTGATG AGAGGAACCT GATGCAGCAG 
GAGCTCCGGT CTATGAAGCA GAACATTTTC CTTTTCTACG TCAAACTCAG ATGGCTGCTG 
AGGCACTGGC GACAAGGGAA GCAGATGGAG GAAGAAGGCG AGGATTTCAC TGAGAGCGAG 
CATCCAGAGA CCCTCCCCAG GCTTGGTGAG CTTGGAGTGC AGGGGGACCC GAAGGGGGAC 
GGCCCAGACC AAGATGACAA CGGTCCGGGC TGCGGCTTTC CGCTGGGAGA GCATCCTCCA 
CACTCCCCTG TGCAGATTGG TGACCATGGA TCGAGACTAC AGCCTACACA TGGGAGACAG 
CTCCACAGGC AGGTGGTGGA AAACCAGCAG CTGTTCAGTG CACTCAAGAG CCTGCTGGAG 
GACCTGCGCG CCGAGCTGCG GGAGGACGAG CATGCACGGC GGCGGCTGCA GCAGCAGTAC 
GCCAGCGACA AAGCCGCCTG GGACGTGGAG TGGGCTGCAC TTACCTGCCG CCTGGAACAG 
GTGCCTGCCT GCGGGGTCTG TCCTGGGTCT CCCCAGCTCC TCCTCTGCCA TCTGGGCCAG 
CTGGAAGAGA AGACTGAGAA GAATTTGGGA GAAGCATGCT CTTCCACCGA CAGCAAAGGG 
GCATTCAAGA AGGAAAGAGA GATGCATCAG AAGCTCCTCA CTGACAGTCA TGGCCTGGTC 
ATGGACCTGC GCTGGCAGAT CCATCACAGT GAGAAGAACT GGAGCCGGGA GAAGGTGGAA 
CTTCTTAGCC GTCTAGACAG AGACCGGCAG GAATGGGAGC AGCAGAAGAA GGAGCTCTTG 
TGGAGAATAG AACAGTTGCA GAAAGAAAAC AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT 
GATCAAAAAG AAGGCACCAT CCGCCCCTTT GCCCATCCAG GAAGCCCCCG AATGCCCCGT 
CCCTTGGGGA TGTGGTCCTG TGCAGACACC GACTCCACCC CATTCGAAGA CCGGCCGCTG 
TCCAAGCTGA AGGAATCAGA CAGATGTTCA GCACGGGAAA ACCTCTACTT GGACGCCTTG 
TCTCTAGATG ACGAGCCAGA GGAGCCTCCA GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC 
TGCCTCCCCC AGGAAGAAGA AAATCACAAG GGAAATCTTC AAAGGGCCGT GTCTGTGTCC 
TCCATGTCTG AGTTCCAGCG TCTGATGGAC GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC 
CCCGCCACCA GCAGCAAGGA GGACATCACT CCGCCCCTGT CTCCTGATGA TCTGAAGTAC 
ATCGAGGAGT TCAACAGCAA GAACTGGGAC GAGAGGCCCC CGGAGCCCTG GGCAGACAGG 
ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG 
TACCTCACCA CGAGTGTCAC CATGACTACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCCC TTCGGAGCCC CATGTGCGCG GAGCAGGCCG GGGTGCGGGT GCTGCACAGC 
CCGCCCGCGG TCCGTAGGAT GGATGGCATC GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG 
GCCGAGCCCG AGTGCCCGTT TCCAACTAGC AGAGCCAGGG GGGGCACTGG AGACACAAAA 
GGGGGTCCCT CAGAACACGT GCTCAGCAGG TGGCCTTGCG CCCCCTCCAG GCACCCCCGA 
GACTATGTGG AGGGAGGGCT GCGCCCCCTC GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT 
GCCTCCTCGC TGCACGGCCT GCAGATGTCC AAGAACATGA GTGATGACAT GAAGGAGGTG 
GCCTTCTCGG TCAGGAACGC CATCTGCTCT GGCCCCCCCA AGCCTCCGCT CAAGGACATG 
GCCTGCCAGA CCAATGGGTC CCGGACGACG GGAACACAGA CCGTCCAGAC CATCACCATT 
GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC GCCGTCACCA GCAGCCCCCA CAAGTGTCTC 
ACACCCAAGG CCGGGGGCGG GGCCACGCCC GCGTCATCTC CTTCCCGTGG CCTTCGGAGC 
CGGCAGATGG CCCCCGCCAT CGAGAAGGTG CAGGCCAAGT TCGAACGCAC GTGCTGCTCC 
CCCAAGTACG GGTCTCCCAA GCTGCAGAGG AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC 
AGCAGGACCT CCCTGGGGCT GGCCCCAAAG GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC 
ACCACCACGA GGGAGAGCCC GGTGCACACC GCCATCAACG ATGGCCTCTC CAGCCTCTTC 
AACATCATCG ACCACAGCCC CGTGGTGCAG GACCCCTTCC AGAAGGGGAC TCGGGCCGGG 
AGCCGATCTC GCTCAGCAGA ACCCCGGCCC GAGCTGGGCC CAGGCCAGGA AACGGGCCCC 
AGTTCCCGGG GCCGGTCCCC CAGCCCCATC GGGGCTGGCT CAGAGACTTG GAGGGAGGAA 
GGGGGAGAGG GCACGCCCTT GAGGCAGGAC TTATCCGCTC CCCCTGGTTA CACGTTCACT 
GAGAACGTAG CCCGGATCCT CAACAAGAAG CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG 
AGGCAGGCCC CCCACGGGCC CCCAAGTCTT AACAGTGACA GCCACATGGG AGAAACCATC 
AAAGCTGAAC CAGGGTCCAT CGAGGAACTA CCTTGTTCCG CACTAGCTCC ATCCCTAGAA 
CCCTGCTTCT CCAGGCCCGA GAGACCAGCA AACCGTCGCC TTCCGTCCCG TTGGGCCTCA 
CATTCCCCCA CTGCCTCACT GGCTCAGTCA CCCGGAGACC CAGCCTCCTT GGAGGAGCTC 
GGCGATGAGG AGCCACCGGG GGAGAAGACA CACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421623.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277331.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.

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CloneID	OCe289882
Clone ID Related Accession (Same CDS sequence)	XM_022421623.1
Accession Version	XM_022421623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAT GGAGGAGGAG 
CACCTGTACG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAGAACACC 
CTCCATGAGC AAACCTGGAG TGATGAGAGG AACCTGATGC AGCAGGAGCT CCGGTCTATG 
AAGCAGAACA TTTTCCTTTT CTACGTCAAA CTCAGATGGC TGCTGAGGCA CTGGCGACAA 
GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT TTCACTGAGA GCGAGCATCC AGAGACCCTC 
CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG GACCCGAAGG GGGACGGCCC AGACCAAGAT 
GACAACGGTC CGGGCTGCGG CTTTCCGCTG GGAGAGCATC CTCCACACTC CCCTGTGCAG 
ATTGGTGACC ATGGATCGAG ACTACAGCCT ACACATGGGA GACAGCTCCA CAGGCAGGTG 
GTGGAAAACC AGCAGCTGTT CAGTGCACTC AAGAGCCTGC TGGAGGACCT GCGCGCCGAG 
CTGCGGGAGG ACGAGCATGC ACGGCGGCGG CTGCAGCAGC AGTACGCCAG CGACAAAGCC 
GCCTGGGACG TGGAGTGGGC TGCACTTACC TGCCGCCTGG AACAGGTGCC TGCCTGCGGG 
GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC TGCCATCTGG GCCAGCTGGA AGAGAAGACT 
GAGAAGAATT TGGGAGAAGC ATGCTCTTCC ACCGACAGCA AAGGGGCATT CAAGAAGGAA 
AGAGAGATGC ATCAGAAGCT CCTCACTGAC AGTCATGGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGTGAGAA GAACTGGAGC CGGGAGAAGG TGGAACTTCT TAGCCGTCTA 
GACAGAGACC GGCAGGAATG GGAGCAGCAG AAGAAGGAGC TCTTGTGGAG AATAGAACAG 
TTGCAGAAAG AAAACAGTCC CCGGAGAAGT GGCAGTTTCC TCTGTGATCA AAAAGAAGGC 
ACCATCCGCC CCTTTGCCCA TCCAGGAAGC CCCCGAATGC CCCGTCCCTT GGGGATGTGG 
TCCTGTGCAG ACACCGACTC CACCCCATTC GAAGACCGGC CGCTGTCCAA GCTGAAGGAA 
TCAGACAGAT GTTCAGCACG GGAAAACCTC TACTTGGACG CCTTGTCTCT AGATGACGAG 
CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG CGGGAGTTCA GGAACTGCCT CCCCCAGGAA 
GAAGAAAATC ACAAGGGAAA TCTTCAAAGG GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC 
CAGCGTCTGA TGGACGTTTC TCCCTTCCTG CCGGAGAAGG GCGTCCCCGC CACCAGCAGC 
AAGGAGGACA TCACTCCGCC CCTGTCTCCT GATGATCTGA AGTACATCGA GGAGTTCAAC 
AGCAAGAACT GGGACGAGAG GCCCCCGGAG CCCTGGGCAG ACAGGACCGA GGGGGGGCGG 
GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC CCTGACTCCT CCTGGTACCT CACCACGAGT 
GTCACCATGA CTACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCCCTTCGG 
AGCCCCATGT GCGCGGAGCA GGCCGGGGTG CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT 
AGGATGGATG GCATCGTGGT GGCGGGCAGT GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC 
CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC ACTGGAGACA CAAAAGGGGG TCCCTCAGAA 
CACGTGCTCA GCAGGTGGCC TTGCGCCCCC TCCAGGCACC CCCGAGACTA TGTGGAGGGA 
GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC 
GGCCTGCAGA TGTCCAAGAA CATGAGTGAT GACATGAAGG AGGTGGCCTT CTCGGTCAGG 
AACGCCATCT GCTCTGGCCC CCCCAAGCCT CCGCTCAAGG ACATGGCCTG CCAGACCAAT 
GGGTCCCGGA CGACGGGAAC ACAGACCGTC CAGACCATCA CCATTGGCCT GCAGACCGAA 
GCCCTGCGTG GCAGCGCCGT CACCAGCAGC CCCCACAAGT GTCTCACACC CAAGGCCGGG 
GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC CGTGGCCTTC GGAGCCGGCA GATGGCCCCC 
GCCATCGAGA AGGTGCAGGC CAAGTTCGAA CGCACGTGCT GCTCCCCCAA GTACGGGTCT 
CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA GCTGAGCCGC CCAACAGCAG GACCTCCCTG 
GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC GCCTGGGCCC GTTCCACCAC CACGAGGGAG 
AGCCCGGTGC ACACCGCCAT CAACGATGGC CTCTCCAGCC TCTTCAACAT CATCGACCAC 
AGCCCCGTGG TGCAGGACCC CTTCCAGAAG GGGACTCGGG CCGGGAGCCG ATCTCGCTCA 
GCAGAACCCC GGCCCGAGCT GGGCCCAGGC CAGGAAACGG GCCCCAGTTC CCGGGGCCGG 
TCCCCCAGCC CCATCGGGGC TGGCTCAGAG ACTTGGAGGG AGGAAGGGGG AGAGGGCACG 
CCCTTGAGGC AGGACTTATC CGCTCCCCCT GGTTACACGT TCACTGAGAA CGTAGCCCGG 
ATCCTCAACA AGAAGCTGTT GGAGCATGCC TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC 
GGGCCCCCAA GTCTTAACAG TGACAGCCAC ATGGGAGAAA CCATCAAAGC TGAACCAGGG 
TCCATCGAGG AACTACCTTG TTCCGCACTA GCTCCATCCC TAGAACCCTG CTTCTCCAGG 
CCCGAGAGAC CAGCAAACCG TCGCCTTCCG TCCCGTTGGG CCTCACATTC CCCCACTGCC 
TCACTGGCTC AGTCACCCGG AGACCCAGCC TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA 
CCGGGGGAGA AGACACACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277331.1
CDS	117..5120
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQQSGGTERLGNCRSTTKTQRKLAPRRKDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLEMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQVPACGVCPGSPQLLLCHLGQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421623.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421623.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAT GGAGGAGGAG 
CACCTGTACG CCTTGAGGTG GAAAGAACTG GAAATGCACA GCCTGGCTTT GCAGAACACC 
CTCCATGAGC AAACCTGGAG TGATGAGAGG AACCTGATGC AGCAGGAGCT CCGGTCTATG 
AAGCAGAACA TTTTCCTTTT CTACGTCAAA CTCAGATGGC TGCTGAGGCA CTGGCGACAA 
GGGAAGCAGA TGGAGGAAGA AGGCGAGGAT TTCACTGAGA GCGAGCATCC AGAGACCCTC 
CCCAGGCTTG GTGAGCTTGG AGTGCAGGGG GACCCGAAGG GGGACGGCCC AGACCAAGAT 
GACAACGGTC CGGGCTGCGG CTTTCCGCTG GGAGAGCATC CTCCACACTC CCCTGTGCAG 
ATTGGTGACC ATGGATCGAG ACTACAGCCT ACACATGGGA GACAGCTCCA CAGGCAGGTG 
GTGGAAAACC AGCAGCTGTT CAGTGCACTC AAGAGCCTGC TGGAGGACCT GCGCGCCGAG 
CTGCGGGAGG ACGAGCATGC ACGGCGGCGG CTGCAGCAGC AGTACGCCAG CGACAAAGCC 
GCCTGGGACG TGGAGTGGGC TGCACTTACC TGCCGCCTGG AACAGGTGCC TGCCTGCGGG 
GTCTGTCCTG GGTCTCCCCA GCTCCTCCTC TGCCATCTGG GCCAGCTGGA AGAGAAGACT 
GAGAAGAATT TGGGAGAAGC ATGCTCTTCC ACCGACAGCA AAGGGGCATT CAAGAAGGAA 
AGAGAGATGC ATCAGAAGCT CCTCACTGAC AGTCATGGCC TGGTCATGGA CCTGCGCTGG 
CAGATCCATC ACAGTGAGAA GAACTGGAGC CGGGAGAAGG TGGAACTTCT TAGCCGTCTA 
GACAGAGACC GGCAGGAATG GGAGCAGCAG AAGAAGGAGC TCTTGTGGAG AATAGAACAG 
TTGCAGAAAG AAAACAGTCC CCGGAGAAGT GGCAGTTTCC TCTGTGATCA AAAAGAAGGC 
ACCATCCGCC CCTTTGCCCA TCCAGGAAGC CCCCGAATGC CCCGTCCCTT GGGGATGTGG 
TCCTGTGCAG ACACCGACTC CACCCCATTC GAAGACCGGC CGCTGTCCAA GCTGAAGGAA 
TCAGACAGAT GTTCAGCACG GGAAAACCTC TACTTGGACG CCTTGTCTCT AGATGACGAG 
CCAGAGGAGC CTCCAGCCCG AGAGCCCCAG CGGGAGTTCA GGAACTGCCT CCCCCAGGAA 
GAAGAAAATC ACAAGGGAAA TCTTCAAAGG GCCGTGTCTG TGTCCTCCAT GTCTGAGTTC 
CAGCGTCTGA TGGACGTTTC TCCCTTCCTG CCGGAGAAGG GCGTCCCCGC CACCAGCAGC 
AAGGAGGACA TCACTCCGCC CCTGTCTCCT GATGATCTGA AGTACATCGA GGAGTTCAAC 
AGCAAGAACT GGGACGAGAG GCCCCCGGAG CCCTGGGCAG ACAGGACCGA GGGGGGGCGG 
GCAGGGAGCG AGGCTGGTGC AGAGCCTTTC CCTGACTCCT CCTGGTACCT CACCACGAGT 
GTCACCATGA CTACGGACAC CATGACCAGC CCAGAGCACT GCCAGAAGCA GCCCCTTCGG 
AGCCCCATGT GCGCGGAGCA GGCCGGGGTG CGGGTGCTGC ACAGCCCGCC CGCGGTCCGT 
AGGATGGATG GCATCGTGGT GGCGGGCAGT GAGGGCAAGG GCCGGGCCGA GCCCGAGTGC 
CCGTTTCCAA CTAGCAGAGC CAGGGGGGGC ACTGGAGACA CAAAAGGGGG TCCCTCAGAA 
CACGTGCTCA GCAGGTGGCC TTGCGCCCCC TCCAGGCACC CCCGAGACTA TGTGGAGGGA 
GGGCTGCGCC CCCTCGAAAG TGCCCTCTGC GCCCCCCTGG GTTTTGCCTC CTCGCTGCAC 
GGCCTGCAGA TGTCCAAGAA CATGAGTGAT GACATGAAGG AGGTGGCCTT CTCGGTCAGG 
AACGCCATCT GCTCTGGCCC CCCCAAGCCT CCGCTCAAGG ACATGGCCTG CCAGACCAAT 
GGGTCCCGGA CGACGGGAAC ACAGACCGTC CAGACCATCA CCATTGGCCT GCAGACCGAA 
GCCCTGCGTG GCAGCGCCGT CACCAGCAGC CCCCACAAGT GTCTCACACC CAAGGCCGGG 
GGCGGGGCCA CGCCCGCGTC ATCTCCTTCC CGTGGCCTTC GGAGCCGGCA GATGGCCCCC 
GCCATCGAGA AGGTGCAGGC CAAGTTCGAA CGCACGTGCT GCTCCCCCAA GTACGGGTCT 
CCCAAGCTGC AGAGGAAGCC CCTCCCCAAA GCTGAGCCGC CCAACAGCAG GACCTCCCTG 
GGGCTGGCCC CAAAGGGGTG CAGCGAGTCC GCCTGGGCCC GTTCCACCAC CACGAGGGAG 
AGCCCGGTGC ACACCGCCAT CAACGATGGC CTCTCCAGCC TCTTCAACAT CATCGACCAC 
AGCCCCGTGG TGCAGGACCC CTTCCAGAAG GGGACTCGGG CCGGGAGCCG ATCTCGCTCA 
GCAGAACCCC GGCCCGAGCT GGGCCCAGGC CAGGAAACGG GCCCCAGTTC CCGGGGCCGG 
TCCCCCAGCC CCATCGGGGC TGGCTCAGAG ACTTGGAGGG AGGAAGGGGG AGAGGGCACG 
CCCTTGAGGC AGGACTTATC CGCTCCCCCT GGTTACACGT TCACTGAGAA CGTAGCCCGG 
ATCCTCAACA AGAAGCTGTT GGAGCATGCC TTAAAGGAGG AGAGGAGGCA GGCCCCCCAC 
GGGCCCCCAA GTCTTAACAG TGACAGCCAC ATGGGAGAAA CCATCAAAGC TGAACCAGGG 
TCCATCGAGG AACTACCTTG TTCCGCACTA GCTCCATCCC TAGAACCCTG CTTCTCCAGG 
CCCGAGAGAC CAGCAAACCG TCGCCTTCCG TCCCGTTGGG CCTCACATTC CCCCACTGCC 
TCACTGGCTC AGTCACCCGG AGACCCAGCC TCCTTGGAGG AGCTCGGCGA TGAGGAGCCA 
CCGGGGGAGA AGACACACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421624.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277332.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.

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CloneID	OCe289883
Clone ID Related Accession (Same CDS sequence)	XM_022421624.1
Accession Version	XM_022421624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC 
CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGAACCAAAC TGTCTTACTA 
ACTGCCCCCT GGGAACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277332.1
CDS	119..5059
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQQSGGTERLGNCRSTTKTQRKLAPRRKDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQVPACGVCPGSPQLLLCHLGQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIENQTVLLTAPWEL

Target ORF information:

RefSeq Version	XM_022421624.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421624.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC 
CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGAACCAAAC TGTCTTACTA 
ACTGCCCCCT GGGAACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421625.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277333.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X9, mRNA.

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CloneID	OCe289884
Clone ID Related Accession (Same CDS sequence)	XM_022421625.1
Accession Version	XM_022421625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGAACCAAAC TGTCTTACTA 
ACTGCCCCCT GGGAACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277333.1
CDS	119..4999
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQQSGGTERLGNCRSTTKTQRKLAPRRKDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIENQTVLLTAPWEL

Target ORF information:

RefSeq Version	XM_022421625.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421625.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGAACCAAAC TGTCTTACTA 
ACTGCCCCCT GGGAACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421626.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277334.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe289885
Clone ID Related Accession (Same CDS sequence)	XM_022421626.1
Accession Version	XM_022421626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGATGG AGGAGGAGCA CCTGTACGCC 
TTGAGGTGGA AAGAACTGGA AATGCACAGC CTGGCTTTGC AGAACACCCT CCATGAGCAA 
ACCTGGAGTG ATGAGAGGAA CCTGATGCAG CAGGAGCTCC GGTCTATGAA GCAGAACATT 
TTCCTTTTCT ACGTCAAACT CAGATGGCTG CTGAGGCACT GGCGACAAGG GAAGCAGATG 
GAGGAAGAAG GCGAGGATTT CACTGAGAGC GAGCATCCAG AGACCCTCCC CAGGCTTGGT 
GAGCTTGGAG TGCAGGGGGA CCCGAAGGGG GACGGCCCAG ACCAAGATGA CAACGGTCCG 
GGCTGCGGCT TTCCGCTGGG AGAGCATCCT CCACACTCCC CTGTGCAGAT TGGTGACCAT 
GGATCGAGAC TACAGCCTAC ACATGGGAGA CAGCTCCACA GGCAGGTGGT GGAAAACCAG 
CAGCTGTTCA GTGCACTCAA GAGCCTGCTG GAGGACCTGC GCGCCGAGCT GCGGGAGGAC 
GAGCATGCAC GGCGGCGGCT GCAGCAGCAG TACGCCAGCG ACAAAGCCGC CTGGGACGTG 
GAGTGGGCTG CACTTACCTG CCGCCTGGAA CAGGTGCCTG CCTGCGGGGT CTGTCCTGGG 
TCTCCCCAGC TCCTCCTCTG CCATCTGGGC CAGCTGGAAG AGAAGACTGA GAAGAATTTG 
GGAGAAGCAT GCTCTTCCAC CGACAGCAAA GGGGCATTCA AGAAGGAAAG AGAGATGCAT 
CAGAAGCTCC TCACTGACAG TCATGGCCTG GTCATGGACC TGCGCTGGCA GATCCATCAC 
AGTGAGAAGA ACTGGAGCCG GGAGAAGGTG GAACTTCTTA GCCGTCTAGA CAGAGACCGG 
CAGGAATGGG AGCAGCAGAA GAAGGAGCTC TTGTGGAGAA TAGAACAGTT GCAGAAAGAA 
AACAGTCCCC GGAGAAGTGG CAGTTTCCTC TGTGATCAAA AAGAAGGCAC CATCCGCCCC 
TTTGCCCATC CAGGAAGCCC CCGAATGCCC CGTCCCTTGG GGATGTGGTC CTGTGCAGAC 
ACCGACTCCA CCCCATTCGA AGACCGGCCG CTGTCCAAGC TGAAGGAATC AGACAGATGT 
TCAGCACGGG AAAACCTCTA CTTGGACGCC TTGTCTCTAG ATGACGAGCC AGAGGAGCCT 
CCAGCCCGAG AGCCCCAGCG GGAGTTCAGG AACTGCCTCC CCCAGGAAGA AGAAAATCAC 
AAGGGAAATC TTCAAAGGGC CGTGTCTGTG TCCTCCATGT CTGAGTTCCA GCGTCTGATG 
GACGTTTCTC CCTTCCTGCC GGAGAAGGGC GTCCCCGCCA CCAGCAGCAA GGAGGACATC 
ACTCCGCCCC TGTCTCCTGA TGATCTGAAG TACATCGAGG AGTTCAACAG CAAGAACTGG 
GACGAGAGGC CCCCGGAGCC CTGGGCAGAC AGGACCGAGG GGGGGCGGGC AGGGAGCGAG 
GCTGGTGCAG AGCCTTTCCC TGACTCCTCC TGGTACCTCA CCACGAGTGT CACCATGACT 
ACGGACACCA TGACCAGCCC AGAGCACTGC CAGAAGCAGC CCCTTCGGAG CCCCATGTGC 
GCGGAGCAGG CCGGGGTGCG GGTGCTGCAC AGCCCGCCCG CGGTCCGTAG GATGGATGGC 
ATCGTGGTGG CGGGCAGTGA GGGCAAGGGC CGGGCCGAGC CCGAGTGCCC GTTTCCAACT 
AGCAGAGCCA GGGGGGGCAC TGGAGACACA AAAGGGGGTC CCTCAGAACA CGTGCTCAGC 
AGGTGGCCTT GCGCCCCCTC CAGGCACCCC CGAGACTATG TGGAGGGAGG GCTGCGCCCC 
CTCGAAAGTG CCCTCTGCGC CCCCCTGGGT TTTGCCTCCT CGCTGCACGG CCTGCAGATG 
TCCAAGAACA TGAGTGATGA CATGAAGGAG GTGGCCTTCT CGGTCAGGAA CGCCATCTGC 
TCTGGCCCCC CCAAGCCTCC GCTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ACGGGAACAC AGACCGTCCA GACCATCACC ATTGGCCTGC AGACCGAAGC CCTGCGTGGC 
AGCGCCGTCA CCAGCAGCCC CCACAAGTGT CTCACACCCA AGGCCGGGGG CGGGGCCACG 
CCCGCGTCAT CTCCTTCCCG TGGCCTTCGG AGCCGGCAGA TGGCCCCCGC CATCGAGAAG 
GTGCAGGCCA AGTTCGAACG CACGTGCTGC TCCCCCAAGT ACGGGTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC TGAGCCGCCC AACAGCAGGA CCTCCCTGGG GCTGGCCCCA 
AAGGGGTGCA GCGAGTCCGC CTGGGCCCGT TCCACCACCA CGAGGGAGAG CCCGGTGCAC 
ACCGCCATCA ACGATGGCCT CTCCAGCCTC TTCAACATCA TCGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GACTCGGGCC GGGAGCCGAT CTCGCTCAGC AGAACCCCGG 
CCCGAGCTGG GCCCAGGCCA GGAAACGGGC CCCAGTTCCC GGGGCCGGTC CCCCAGCCCC 
ATCGGGGCTG GCTCAGAGAC TTGGAGGGAG GAAGGGGGAG AGGGCACGCC CTTGAGGCAG 
GACTTATCCG CTCCCCCTGG TTACACGTTC ACTGAGAACG TAGCCCGGAT CCTCAACAAG 
AAGCTGTTGG AGCATGCCTT AAAGGAGGAG AGGAGGCAGG CCCCCCACGG GCCCCCAAGT 
CTTAACAGTG ACAGCCACAT GGGAGAAACC ATCAAAGCTG AACCAGGGTC CATCGAGGAA 
CTACCTTGTT CCGCACTAGC TCCATCCCTA GAACCCTGCT TCTCCAGGCC CGAGAGACCA 
GCAAACCGTC GCCTTCCGTC CCGTTGGGCC TCACATTCCC CCACTGCCTC ACTGGCTCAG 
TCACCCGGAG ACCCAGCCTC CTTGGAGGAG CTCGGCGATG AGGAGCCACC GGGGGAGAAG 
ACACACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277334.1
CDS	117..4988
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQQSGGTERLGNCRSTTKTQRKLAPRRKDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQVPACGVCPGSPQLLLCHLGQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421626.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421626.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGATGG AGGAGGAGCA CCTGTACGCC 
TTGAGGTGGA AAGAACTGGA AATGCACAGC CTGGCTTTGC AGAACACCCT CCATGAGCAA 
ACCTGGAGTG ATGAGAGGAA CCTGATGCAG CAGGAGCTCC GGTCTATGAA GCAGAACATT 
TTCCTTTTCT ACGTCAAACT CAGATGGCTG CTGAGGCACT GGCGACAAGG GAAGCAGATG 
GAGGAAGAAG GCGAGGATTT CACTGAGAGC GAGCATCCAG AGACCCTCCC CAGGCTTGGT 
GAGCTTGGAG TGCAGGGGGA CCCGAAGGGG GACGGCCCAG ACCAAGATGA CAACGGTCCG 
GGCTGCGGCT TTCCGCTGGG AGAGCATCCT CCACACTCCC CTGTGCAGAT TGGTGACCAT 
GGATCGAGAC TACAGCCTAC ACATGGGAGA CAGCTCCACA GGCAGGTGGT GGAAAACCAG 
CAGCTGTTCA GTGCACTCAA GAGCCTGCTG GAGGACCTGC GCGCCGAGCT GCGGGAGGAC 
GAGCATGCAC GGCGGCGGCT GCAGCAGCAG TACGCCAGCG ACAAAGCCGC CTGGGACGTG 
GAGTGGGCTG CACTTACCTG CCGCCTGGAA CAGGTGCCTG CCTGCGGGGT CTGTCCTGGG 
TCTCCCCAGC TCCTCCTCTG CCATCTGGGC CAGCTGGAAG AGAAGACTGA GAAGAATTTG 
GGAGAAGCAT GCTCTTCCAC CGACAGCAAA GGGGCATTCA AGAAGGAAAG AGAGATGCAT 
CAGAAGCTCC TCACTGACAG TCATGGCCTG GTCATGGACC TGCGCTGGCA GATCCATCAC 
AGTGAGAAGA ACTGGAGCCG GGAGAAGGTG GAACTTCTTA GCCGTCTAGA CAGAGACCGG 
CAGGAATGGG AGCAGCAGAA GAAGGAGCTC TTGTGGAGAA TAGAACAGTT GCAGAAAGAA 
AACAGTCCCC GGAGAAGTGG CAGTTTCCTC TGTGATCAAA AAGAAGGCAC CATCCGCCCC 
TTTGCCCATC CAGGAAGCCC CCGAATGCCC CGTCCCTTGG GGATGTGGTC CTGTGCAGAC 
ACCGACTCCA CCCCATTCGA AGACCGGCCG CTGTCCAAGC TGAAGGAATC AGACAGATGT 
TCAGCACGGG AAAACCTCTA CTTGGACGCC TTGTCTCTAG ATGACGAGCC AGAGGAGCCT 
CCAGCCCGAG AGCCCCAGCG GGAGTTCAGG AACTGCCTCC CCCAGGAAGA AGAAAATCAC 
AAGGGAAATC TTCAAAGGGC CGTGTCTGTG TCCTCCATGT CTGAGTTCCA GCGTCTGATG 
GACGTTTCTC CCTTCCTGCC GGAGAAGGGC GTCCCCGCCA CCAGCAGCAA GGAGGACATC 
ACTCCGCCCC TGTCTCCTGA TGATCTGAAG TACATCGAGG AGTTCAACAG CAAGAACTGG 
GACGAGAGGC CCCCGGAGCC CTGGGCAGAC AGGACCGAGG GGGGGCGGGC AGGGAGCGAG 
GCTGGTGCAG AGCCTTTCCC TGACTCCTCC TGGTACCTCA CCACGAGTGT CACCATGACT 
ACGGACACCA TGACCAGCCC AGAGCACTGC CAGAAGCAGC CCCTTCGGAG CCCCATGTGC 
GCGGAGCAGG CCGGGGTGCG GGTGCTGCAC AGCCCGCCCG CGGTCCGTAG GATGGATGGC 
ATCGTGGTGG CGGGCAGTGA GGGCAAGGGC CGGGCCGAGC CCGAGTGCCC GTTTCCAACT 
AGCAGAGCCA GGGGGGGCAC TGGAGACACA AAAGGGGGTC CCTCAGAACA CGTGCTCAGC 
AGGTGGCCTT GCGCCCCCTC CAGGCACCCC CGAGACTATG TGGAGGGAGG GCTGCGCCCC 
CTCGAAAGTG CCCTCTGCGC CCCCCTGGGT TTTGCCTCCT CGCTGCACGG CCTGCAGATG 
TCCAAGAACA TGAGTGATGA CATGAAGGAG GTGGCCTTCT CGGTCAGGAA CGCCATCTGC 
TCTGGCCCCC CCAAGCCTCC GCTCAAGGAC ATGGCCTGCC AGACCAATGG GTCCCGGACG 
ACGGGAACAC AGACCGTCCA GACCATCACC ATTGGCCTGC AGACCGAAGC CCTGCGTGGC 
AGCGCCGTCA CCAGCAGCCC CCACAAGTGT CTCACACCCA AGGCCGGGGG CGGGGCCACG 
CCCGCGTCAT CTCCTTCCCG TGGCCTTCGG AGCCGGCAGA TGGCCCCCGC CATCGAGAAG 
GTGCAGGCCA AGTTCGAACG CACGTGCTGC TCCCCCAAGT ACGGGTCTCC CAAGCTGCAG 
AGGAAGCCCC TCCCCAAAGC TGAGCCGCCC AACAGCAGGA CCTCCCTGGG GCTGGCCCCA 
AAGGGGTGCA GCGAGTCCGC CTGGGCCCGT TCCACCACCA CGAGGGAGAG CCCGGTGCAC 
ACCGCCATCA ACGATGGCCT CTCCAGCCTC TTCAACATCA TCGACCACAG CCCCGTGGTG 
CAGGACCCCT TCCAGAAGGG GACTCGGGCC GGGAGCCGAT CTCGCTCAGC AGAACCCCGG 
CCCGAGCTGG GCCCAGGCCA GGAAACGGGC CCCAGTTCCC GGGGCCGGTC CCCCAGCCCC 
ATCGGGGCTG GCTCAGAGAC TTGGAGGGAG GAAGGGGGAG AGGGCACGCC CTTGAGGCAG 
GACTTATCCG CTCCCCCTGG TTACACGTTC ACTGAGAACG TAGCCCGGAT CCTCAACAAG 
AAGCTGTTGG AGCATGCCTT AAAGGAGGAG AGGAGGCAGG CCCCCCACGG GCCCCCAAGT 
CTTAACAGTG ACAGCCACAT GGGAGAAACC ATCAAAGCTG AACCAGGGTC CATCGAGGAA 
CTACCTTGTT CCGCACTAGC TCCATCCCTA GAACCCTGCT TCTCCAGGCC CGAGAGACCA 
GCAAACCGTC GCCTTCCGTC CCGTTGGGCC TCACATTCCC CCACTGCCTC ACTGGCTCAG 
TCACCCGGAG ACCCAGCCTC CTTGGAGGAG CTCGGCGATG AGGAGCCACC GGGGGAGAAG 
ACACACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421627.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277335.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe289886
Clone ID Related Accession (Same CDS sequence)	XM_022421627.1
Accession Version	XM_022421627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC 
CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA 
CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT 
CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA 
GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277335.1
CDS	132..5228
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQQSGGTERLGNCRSTTKTQRKLAPRRKDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQVPACGVCPGSPQLLLCHLGQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421627.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421627.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
CAGAGTGGTG GAACCGAGCG CCTTGGAAAC TGCAGGTCTA CAACCAAAAC TCAAAGGAAA 
CTGGCACCCC GGCGCAAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC 
CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA 
CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT 
CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA 
GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421628.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277336.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X12, mRNA.

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CloneID	OCe289887
Clone ID Related Accession (Same CDS sequence)	XM_022421628.1
Accession Version	XM_022421628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCAGG ACACAAAGAG TTCCCTGAAA AGAAGAGGCA ACCGAGAAAC ATACAAAGAA 
AAGAAAACCT TTAACCAGCA GAGTGGTGGA ACCGAGCGCC TTGGAAACTG CAGGTCTACA 
ACCAAAACTC AAAGGAAACT GGCACCCCGG CGCAAGGATG ACAGTGCCGA TTTGAAGTGC 
CAGCTTCAGT TTGCCAAAGA GGAAGGCTCC CTGATGCGTA AAAAGATGGC CAAGCTGGGG 
AAGGAGAAGG ACGAGCTGGA ACAGGAGCTC CAGAAGTACA AGGCCCTCTA TGGAGACGTG 
GACAGCCCCC TCCCCACGGG GGAAGCCGGG GGGCCGCCCA GCACCAGGGA GGCTGAGCTG 
AAGCTGCGCT TGAAGTTGGT GGAGGAAGAG GCCAACATCT TGGGCCGGAA GATTGTGGAA 
CTGGAGGTGG AGAACCGAGG CCTCAAAGCA GAAATGGAGG ACATGAGGCT CCAGTACGAG 
CGAGAGGGTA CAGGCCGGGA CCACATCACA GGCATTCCTA CCTCACCCTT CGGTGACTCC 
CTGGAGTCCT CGACGGAGCT CCGCCGGCAC CTGCAGTTCG TGGAGGAGGA AGCGGAGCTG 
CTCCGGAGGT CCATATCTGA GATCGAAGAC CACAACCGGC AGCTGACCCA TGAGCTGAAC 
AAGTTCAAGT TTGAGCCTCG GCCGGAGCCA GGGTGGCTGG CGGATGGCGC GGGCAAGGGC 
GTGGGCAGCG GGGCCCCTCT GCAGGAGGAG CTCAAGTCGG CACGGCTACA GATCAATGAG 
CTCAGTGGGA AGGTGCTGAA GCTGCAGTAT GAGAACCGGG TGCTACTGTC CAATGTGCAG 
CGCTATGACC TGGCTGCCCA CCTAGGCCTG CGTGCCCCCA GCCCCCGGGA CAGCGACGCA 
GACAGCGACC AGGGCAAGAA GGAGAGCGAT GGGGAGGAAG GCCGCCAGCC CCAGCCCAAG 
CGGGAGGGCC CTATTGGCGG GGAGAGCGAC TCAGAGGAGA TGTGCGAGAA GACGTCGGGC 
TTCGGCAGCG GAAAGCCATC GGAGGCGAGC GAGCCATGTC CCGCCGAGCT CCTGCGGGCC 
AGGGAGGACA CCGAGTGCCT GGCGAGCATA AAGCACGAGG CTGAGCGCCT GGAGCGAACA 
GTGGAGCGCC TCATCACGGA CACCGCGGGC TTCGGGGACG ATGTGGGGCT GCTGGGCAGT 
GGCGAGCCCT TGCCAGGGCC CGACATCCAG GGGGCGGGGG GGACGGGCGA GGGGGGCAAG 
AAGGAGCCCC CACTGCTGGG GACCATCAAC TCGAGGATGA AGGCTTTCAG GAAGGAGCTG 
CAGGCCTTCC TGGAGCAGGT GAACCGAATC GGGGATGGCC TGCGGGAGCG CCTGGAAGGC 
CTGTCCCCCC TGCCTCACCT CACAGAGTCT TCCAGCTTTC TCTCCACCGT CACTTCCGTG 
TCCCGGGACT CCCCCGTCGG GAACCTGGGG AAGGAGCTGG TCCCGGACCT GCAGTCCAAA 
CTGAGAGGTC AGCTGGAGTG GCAGCCGGGG CAGGAGTGCG GGGACGAGCC AGAGCAGCTG 
CACCGCCGCG CTGATGGGGA CGTCGGGAGC TGCCGGCAGG GAGGCCAGAG CTGCTTCAGT 
CTAGAGAGTG TATCGCTAGA GGAGCTGCAG GGTCAGCTTG CGCAGGCGAC CAGACTGCAT 
CAAGAGGAGA CAGAGAAATT TACAAACAAG ATTCGGAAGA TGGAGGAGGA GCACCTGTAC 
GCCTTGAGGT GGAAAGAACT GGAAATGCAC AGCCTGGCTT TGCAGAACAC CCTCCATGAG 
CAAACCTGGA GTGATGAGAG GAACCTGATG CAGCAGGAGC TCCGGTCTAT GAAGCAGAAC 
ATTTTCCTTT TCTACGTCAA ACTCAGATGG CTGCTGAGGC ACTGGCGACA AGGGAAGCAG 
ATGGAGGAAG AAGGCGAGGA TTTCACTGAG AGCGAGCATC CAGAGACCCT CCCCAGGCTT 
GGTGAGCTTG GAGTGCAGGG GGACCCGAAG GGGGACGGCC CAGACCAAGA TGACAACGGT 
CCGGGCTGCG GCTTTCCGCT GGGAGAGCAT CCTCCACACT CCCCTGTGCA GATTGGTGAC 
CATGGATCGA GACTACAGCC TACACATGGG AGACAGCTCC ACAGGCAGGT GGTGGAAAAC 
CAGCAGCTGT TCAGTGCACT CAAGAGCCTG CTGGAGGACC TGCGCGCCGA GCTGCGGGAG 
GACGAGCATG CACGGCGGCG GCTGCAGCAG CAGTACGCCA GCGACAAAGC CGCCTGGGAC 
GTGGAGTGGG CTGCACTTAC CTGCCGCCTG GAACAGGTGC CTGCCTGCGG GGTCTGTCCT 
GGGTCTCCCC AGCTCCTCCT CTGCCATCTG GGCCAGCTGG AAGAGAAGAC TGAGAAGAAT 
TTGGGAGAAG CATGCTCTTC CACCGACAGC AAAGGGGCAT TCAAGAAGGA AAGAGAGATG 
CATCAGAAGC TCCTCACTGA CAGTCATGGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGTGAGA AGAACTGGAG CCGGGAGAAG GTGGAACTTC TTAGCCGTCT AGACAGAGAC 
CGGCAGGAAT GGGAGCAGCA GAAGAAGGAG CTCTTGTGGA GAATAGAACA GTTGCAGAAA 
GAAAACAGTC CCCGGAGAAG TGGCAGTTTC CTCTGTGATC AAAAAGAAGG CACCATCCGC 
CCCTTTGCCC ATCCAGGAAG CCCCCGAATG CCCCGTCCCT TGGGGATGTG GTCCTGTGCA 
GACACCGACT CCACCCCATT CGAAGACCGG CCGCTGTCCA AGCTGAAGGA ATCAGACAGA 
TGTTCAGCAC GGGAAAACCT CTACTTGGAC GCCTTGTCTC TAGATGACGA GCCAGAGGAG 
CCTCCAGCCC GAGAGCCCCA GCGGGAGTTC AGGAACTGCC TCCCCCAGGA AGAAGAAAAT 
CACAAGGGAA ATCTTCAAAG GGCCGTGTCT GTGTCCTCCA TGTCTGAGTT CCAGCGTCTG 
ATGGACGTTT CTCCCTTCCT GCCGGAGAAG GGCGTCCCCG CCACCAGCAG CAAGGAGGAC 
ATCACTCCGC CCCTGTCTCC TGATGATCTG AAGTACATCG AGGAGTTCAA CAGCAAGAAC 
TGGGACGAGA GGCCCCCGGA GCCCTGGGCA GACAGGACCG AGGGGGGGCG GGCAGGGAGC 
GAGGCTGGTG CAGAGCCTTT CCCTGACTCC TCCTGGTACC TCACCACGAG TGTCACCATG 
ACTACGGACA CCATGACCAG CCCAGAGCAC TGCCAGAAGC AGCCCCTTCG GAGCCCCATG 
TGCGCGGAGC AGGCCGGGGT GCGGGTGCTG CACAGCCCGC CCGCGGTCCG TAGGATGGAT 
GGCATCGTGG TGGCGGGCAG TGAGGGCAAG GGCCGGGCCG AGCCCGAGTG CCCGTTTCCA 
ACTAGCAGAG CCAGGGGGGG CACTGGAGAC ACAAAAGGGG GTCCCTCAGA ACACGTGCTC 
AGCAGGTGGC CTTGCGCCCC CTCCAGGCAC CCCCGAGACT ATGTGGAGGG AGGGCTGCGC 
CCCCTCGAAA GTGCCCTCTG CGCCCCCCTG GGTTTTGCCT CCTCGCTGCA CGGCCTGCAG 
ATGTCCAAGA ACATGAGTGA TGACATGAAG GAGGTGGCCT TCTCGGTCAG GAACGCCATC 
TGCTCTGGCC CCCCCAAGCC TCCGCTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG 
ACGACGGGAA CACAGACCGT CCAGACCATC ACCATTGGCC TGCAGACCGA AGCCCTGCGT 
GGCAGCGCCG TCACCAGCAG CCCCCACAAG TGTCTCACAC CCAAGGCCGG GGGCGGGGCC 
ACGCCCGCGT CATCTCCTTC CCGTGGCCTT CGGAGCCGGC AGATGGCCCC CGCCATCGAG 
AAGGTGCAGG CCAAGTTCGA ACGCACGTGC TGCTCCCCCA AGTACGGGTC TCCCAAGCTG 
CAGAGGAAGC CCCTCCCCAA AGCTGAGCCG CCCAACAGCA GGACCTCCCT GGGGCTGGCC 
CCAAAGGGGT GCAGCGAGTC CGCCTGGGCC CGTTCCACCA CCACGAGGGA GAGCCCGGTG 
CACACCGCCA TCAACGATGG CCTCTCCAGC CTCTTCAACA TCATCGACCA CAGCCCCGTG 
GTGCAGGACC CCTTCCAGAA GGGGACTCGG GCCGGGAGCC GATCTCGCTC AGCAGAACCC 
CGGCCCGAGC TGGGCCCAGG CCAGGAAACG GGCCCCAGTT CCCGGGGCCG GTCCCCCAGC 
CCCATCGGGG CTGGCTCAGA GACTTGGAGG GAGGAAGGGG GAGAGGGCAC GCCCTTGAGG 
CAGGACTTAT CCGCTCCCCC TGGTTACACG TTCACTGAGA ACGTAGCCCG GATCCTCAAC 
AAGAAGCTGT TGGAGCATGC CTTAAAGGAG GAGAGGAGGC AGGCCCCCCA CGGGCCCCCA 
AGTCTTAACA GTGACAGCCA CATGGGAGAA ACCATCAAAG CTGAACCAGG GTCCATCGAG 
GAACTACCTT GTTCCGCACT AGCTCCATCC CTAGAACCCT GCTTCTCCAG GCCCGAGAGA 
CCAGCAAACC GTCGCCTTCC GTCCCGTTGG GCCTCACATT CCCCCACTGC CTCACTGGCT 
CAGTCACCCG GAGACCCAGC CTCCTTGGAG GAGCTCGGCG ATGAGGAGCC ACCGGGGGAG 
AAGACACACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277336.1
CDS	699..5393
Translation	MLQDTKSSLKRRGNRETYKEKKTFNQQSGGTERLGNCRSTTKTQRKLAPRRKDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQVPACGVCPGSPQLLLCHLGQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421628.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421628.1

ORF Insert Sequence:

ATGTTGCAGG ACACAAAGAG TTCCCTGAAA AGAAGAGGCA ACCGAGAAAC ATACAAAGAA 
AAGAAAACCT TTAACCAGCA GAGTGGTGGA ACCGAGCGCC TTGGAAACTG CAGGTCTACA 
ACCAAAACTC AAAGGAAACT GGCACCCCGG CGCAAGGATG ACAGTGCCGA TTTGAAGTGC 
CAGCTTCAGT TTGCCAAAGA GGAAGGCTCC CTGATGCGTA AAAAGATGGC CAAGCTGGGG 
AAGGAGAAGG ACGAGCTGGA ACAGGAGCTC CAGAAGTACA AGGCCCTCTA TGGAGACGTG 
GACAGCCCCC TCCCCACGGG GGAAGCCGGG GGGCCGCCCA GCACCAGGGA GGCTGAGCTG 
AAGCTGCGCT TGAAGTTGGT GGAGGAAGAG GCCAACATCT TGGGCCGGAA GATTGTGGAA 
CTGGAGGTGG AGAACCGAGG CCTCAAAGCA GAAATGGAGG ACATGAGGCT CCAGTACGAG 
CGAGAGGGTA CAGGCCGGGA CCACATCACA GGCATTCCTA CCTCACCCTT CGGTGACTCC 
CTGGAGTCCT CGACGGAGCT CCGCCGGCAC CTGCAGTTCG TGGAGGAGGA AGCGGAGCTG 
CTCCGGAGGT CCATATCTGA GATCGAAGAC CACAACCGGC AGCTGACCCA TGAGCTGAAC 
AAGTTCAAGT TTGAGCCTCG GCCGGAGCCA GGGTGGCTGG CGGATGGCGC GGGCAAGGGC 
GTGGGCAGCG GGGCCCCTCT GCAGGAGGAG CTCAAGTCGG CACGGCTACA GATCAATGAG 
CTCAGTGGGA AGGTGCTGAA GCTGCAGTAT GAGAACCGGG TGCTACTGTC CAATGTGCAG 
CGCTATGACC TGGCTGCCCA CCTAGGCCTG CGTGCCCCCA GCCCCCGGGA CAGCGACGCA 
GACAGCGACC AGGGCAAGAA GGAGAGCGAT GGGGAGGAAG GCCGCCAGCC CCAGCCCAAG 
CGGGAGGGCC CTATTGGCGG GGAGAGCGAC TCAGAGGAGA TGTGCGAGAA GACGTCGGGC 
TTCGGCAGCG GAAAGCCATC GGAGGCGAGC GAGCCATGTC CCGCCGAGCT CCTGCGGGCC 
AGGGAGGACA CCGAGTGCCT GGCGAGCATA AAGCACGAGG CTGAGCGCCT GGAGCGAACA 
GTGGAGCGCC TCATCACGGA CACCGCGGGC TTCGGGGACG ATGTGGGGCT GCTGGGCAGT 
GGCGAGCCCT TGCCAGGGCC CGACATCCAG GGGGCGGGGG GGACGGGCGA GGGGGGCAAG 
AAGGAGCCCC CACTGCTGGG GACCATCAAC TCGAGGATGA AGGCTTTCAG GAAGGAGCTG 
CAGGCCTTCC TGGAGCAGGT GAACCGAATC GGGGATGGCC TGCGGGAGCG CCTGGAAGGC 
CTGTCCCCCC TGCCTCACCT CACAGAGTCT TCCAGCTTTC TCTCCACCGT CACTTCCGTG 
TCCCGGGACT CCCCCGTCGG GAACCTGGGG AAGGAGCTGG TCCCGGACCT GCAGTCCAAA 
CTGAGAGGTC AGCTGGAGTG GCAGCCGGGG CAGGAGTGCG GGGACGAGCC AGAGCAGCTG 
CACCGCCGCG CTGATGGGGA CGTCGGGAGC TGCCGGCAGG GAGGCCAGAG CTGCTTCAGT 
CTAGAGAGTG TATCGCTAGA GGAGCTGCAG GGTCAGCTTG CGCAGGCGAC CAGACTGCAT 
CAAGAGGAGA CAGAGAAATT TACAAACAAG ATTCGGAAGA TGGAGGAGGA GCACCTGTAC 
GCCTTGAGGT GGAAAGAACT GGAAATGCAC AGCCTGGCTT TGCAGAACAC CCTCCATGAG 
CAAACCTGGA GTGATGAGAG GAACCTGATG CAGCAGGAGC TCCGGTCTAT GAAGCAGAAC 
ATTTTCCTTT TCTACGTCAA ACTCAGATGG CTGCTGAGGC ACTGGCGACA AGGGAAGCAG 
ATGGAGGAAG AAGGCGAGGA TTTCACTGAG AGCGAGCATC CAGAGACCCT CCCCAGGCTT 
GGTGAGCTTG GAGTGCAGGG GGACCCGAAG GGGGACGGCC CAGACCAAGA TGACAACGGT 
CCGGGCTGCG GCTTTCCGCT GGGAGAGCAT CCTCCACACT CCCCTGTGCA GATTGGTGAC 
CATGGATCGA GACTACAGCC TACACATGGG AGACAGCTCC ACAGGCAGGT GGTGGAAAAC 
CAGCAGCTGT TCAGTGCACT CAAGAGCCTG CTGGAGGACC TGCGCGCCGA GCTGCGGGAG 
GACGAGCATG CACGGCGGCG GCTGCAGCAG CAGTACGCCA GCGACAAAGC CGCCTGGGAC 
GTGGAGTGGG CTGCACTTAC CTGCCGCCTG GAACAGGTGC CTGCCTGCGG GGTCTGTCCT 
GGGTCTCCCC AGCTCCTCCT CTGCCATCTG GGCCAGCTGG AAGAGAAGAC TGAGAAGAAT 
TTGGGAGAAG CATGCTCTTC CACCGACAGC AAAGGGGCAT TCAAGAAGGA AAGAGAGATG 
CATCAGAAGC TCCTCACTGA CAGTCATGGC CTGGTCATGG ACCTGCGCTG GCAGATCCAT 
CACAGTGAGA AGAACTGGAG CCGGGAGAAG GTGGAACTTC TTAGCCGTCT AGACAGAGAC 
CGGCAGGAAT GGGAGCAGCA GAAGAAGGAG CTCTTGTGGA GAATAGAACA GTTGCAGAAA 
GAAAACAGTC CCCGGAGAAG TGGCAGTTTC CTCTGTGATC AAAAAGAAGG CACCATCCGC 
CCCTTTGCCC ATCCAGGAAG CCCCCGAATG CCCCGTCCCT TGGGGATGTG GTCCTGTGCA 
GACACCGACT CCACCCCATT CGAAGACCGG CCGCTGTCCA AGCTGAAGGA ATCAGACAGA 
TGTTCAGCAC GGGAAAACCT CTACTTGGAC GCCTTGTCTC TAGATGACGA GCCAGAGGAG 
CCTCCAGCCC GAGAGCCCCA GCGGGAGTTC AGGAACTGCC TCCCCCAGGA AGAAGAAAAT 
CACAAGGGAA ATCTTCAAAG GGCCGTGTCT GTGTCCTCCA TGTCTGAGTT CCAGCGTCTG 
ATGGACGTTT CTCCCTTCCT GCCGGAGAAG GGCGTCCCCG CCACCAGCAG CAAGGAGGAC 
ATCACTCCGC CCCTGTCTCC TGATGATCTG AAGTACATCG AGGAGTTCAA CAGCAAGAAC 
TGGGACGAGA GGCCCCCGGA GCCCTGGGCA GACAGGACCG AGGGGGGGCG GGCAGGGAGC 
GAGGCTGGTG CAGAGCCTTT CCCTGACTCC TCCTGGTACC TCACCACGAG TGTCACCATG 
ACTACGGACA CCATGACCAG CCCAGAGCAC TGCCAGAAGC AGCCCCTTCG GAGCCCCATG 
TGCGCGGAGC AGGCCGGGGT GCGGGTGCTG CACAGCCCGC CCGCGGTCCG TAGGATGGAT 
GGCATCGTGG TGGCGGGCAG TGAGGGCAAG GGCCGGGCCG AGCCCGAGTG CCCGTTTCCA 
ACTAGCAGAG CCAGGGGGGG CACTGGAGAC ACAAAAGGGG GTCCCTCAGA ACACGTGCTC 
AGCAGGTGGC CTTGCGCCCC CTCCAGGCAC CCCCGAGACT ATGTGGAGGG AGGGCTGCGC 
CCCCTCGAAA GTGCCCTCTG CGCCCCCCTG GGTTTTGCCT CCTCGCTGCA CGGCCTGCAG 
ATGTCCAAGA ACATGAGTGA TGACATGAAG GAGGTGGCCT TCTCGGTCAG GAACGCCATC 
TGCTCTGGCC CCCCCAAGCC TCCGCTCAAG GACATGGCCT GCCAGACCAA TGGGTCCCGG 
ACGACGGGAA CACAGACCGT CCAGACCATC ACCATTGGCC TGCAGACCGA AGCCCTGCGT 
GGCAGCGCCG TCACCAGCAG CCCCCACAAG TGTCTCACAC CCAAGGCCGG GGGCGGGGCC 
ACGCCCGCGT CATCTCCTTC CCGTGGCCTT CGGAGCCGGC AGATGGCCCC CGCCATCGAG 
AAGGTGCAGG CCAAGTTCGA ACGCACGTGC TGCTCCCCCA AGTACGGGTC TCCCAAGCTG 
CAGAGGAAGC CCCTCCCCAA AGCTGAGCCG CCCAACAGCA GGACCTCCCT GGGGCTGGCC 
CCAAAGGGGT GCAGCGAGTC CGCCTGGGCC CGTTCCACCA CCACGAGGGA GAGCCCGGTG 
CACACCGCCA TCAACGATGG CCTCTCCAGC CTCTTCAACA TCATCGACCA CAGCCCCGTG 
GTGCAGGACC CCTTCCAGAA GGGGACTCGG GCCGGGAGCC GATCTCGCTC AGCAGAACCC 
CGGCCCGAGC TGGGCCCAGG CCAGGAAACG GGCCCCAGTT CCCGGGGCCG GTCCCCCAGC 
CCCATCGGGG CTGGCTCAGA GACTTGGAGG GAGGAAGGGG GAGAGGGCAC GCCCTTGAGG 
CAGGACTTAT CCGCTCCCCC TGGTTACACG TTCACTGAGA ACGTAGCCCG GATCCTCAAC 
AAGAAGCTGT TGGAGCATGC CTTAAAGGAG GAGAGGAGGC AGGCCCCCCA CGGGCCCCCA 
AGTCTTAACA GTGACAGCCA CATGGGAGAA ACCATCAAAG CTGAACCAGG GTCCATCGAG 
GAACTACCTT GTTCCGCACT AGCTCCATCC CTAGAACCCT GCTTCTCCAG GCCCGAGAGA 
CCAGCAAACC GTCGCCTTCC GTCCCGTTGG GCCTCACATT CCCCCACTGC CTCACTGGCT 
CAGTCACCCG GAGACCCAGC CTCCTTGGAG GAGCTCGGCG ATGAGGAGCC ACCGGGGGAG 
AAGACACACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421629.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277337.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X13, mRNA.

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CloneID	OCe289888
Clone ID Related Accession (Same CDS sequence)	XM_022421629.1
Accession Version	XM_022421629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG 
AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT 
TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA 
GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT 
GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC 
GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG 
AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT 
GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG 
GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA 
GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG 
CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG 
AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA 
TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT 
CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC 
CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC 
TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA 
CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC 
CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA 
AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT 
TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG 
CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG 
AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT 
GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC 
ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG 
CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG 
GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA 
GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG 
CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA 
AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG 
AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC 
CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA 
ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC 
GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG 
TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG 
GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG 
CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG 
TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC 
ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC 
CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG 
CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG 
GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA 
TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG 
TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC 
AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GGAACTACCT 
TGTTCCGCAC TAGCTCCATC CCTAGAACCC TGCTTCTCCA GGCCCGAGAG ACCAGCAAAC 
CGTCGCCTTC CGTCCCGTTG GGCCTCACAT TCCCCCACTG CCTCACTGGC TCAGTCACCC 
GGAGACCCAG CCTCCTTGGA GGAGCTCGGC GATGAGGAGC CACCGGGGGA GAAGACACAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277337.1
CDS	128..4993
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLEMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421629.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421629.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG 
AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT 
TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA 
GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT 
GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC 
GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG 
AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT 
GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG 
GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA 
GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG 
CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG 
AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA 
TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT 
CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC 
CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC 
TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA 
CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC 
CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA 
AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT 
TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG 
CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG 
AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT 
GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC 
ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG 
CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG 
GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA 
GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG 
CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA 
AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG 
AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC 
CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA 
ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC 
GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG 
TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG 
GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG 
CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG 
TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC 
ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC 
CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG 
CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG 
GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA 
TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG 
TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC 
AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GGAACTACCT 
TGTTCCGCAC TAGCTCCATC CCTAGAACCC TGCTTCTCCA GGCCCGAGAG ACCAGCAAAC 
CGTCGCCTTC CGTCCCGTTG GGCCTCACAT TCCCCCACTG CCTCACTGGC TCAGTCACCC 
GGAGACCCAG CCTCCTTGGA GGAGCTCGGC GATGAGGAGC CACCGGGGGA GAAGACACAC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421630.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277338.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X14, mRNA.

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CloneID	OCe289889
Clone ID Related Accession (Same CDS sequence)	XM_022421630.1
Accession Version	XM_022421630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCAGG ACACAAAGAG TTCCCTGAAA AGAAGAGGCA ACCGAGAAAC ATACAAAGAA 
AAGAAAACCT TTAACCAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC 
CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA 
CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT 
CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA 
GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277338.1
CDS	698..5314
Translation	MLQDTKSSLKRRGNRETYKEKKTFNQDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQVPACGVCPGSPQLLLCHLGQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_022421630.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421630.1

ORF Insert Sequence:

ATGTTGCAGG ACACAAAGAG TTCCCTGAAA AGAAGAGGCA ACCGAGAAAC ATACAAAGAA 
AAGAAAACCT TTAACCAGGA TGACAGTGCC GATTTGAAGT GCCAGCTTCA GTTTGCCAAA 
GAGGAAGGCT CCCTGATGCG TAAAAAGATG GCCAAGCTGG GGAAGGAGAA GGACGAGCTG 
GAACAGGAGC TCCAGAAGTA CAAGGCCCTC TATGGAGACG TGGACAGCCC CCTCCCCACG 
GGGGAAGCCG GGGGGCCGCC CAGCACCAGG GAGGCTGAGC TGAAGCTGCG CTTGAAGTTG 
GTGGAGGAAG AGGCCAACAT CTTGGGCCGG AAGATTGTGG AACTGGAGGT GGAGAACCGA 
GGCCTCAAAG CAGAAATGGA GGACATGAGG CTCCAGTACG AGCGAGAGGG TACAGGCCGG 
GACCACATCA CAGGCATTCC TACCTCACCC TTCGGTGACT CCCTGGAGTC CTCGACGGAG 
CTCCGCCGGC ACCTGCAGTT CGTGGAGGAG GAAGCGGAGC TGCTCCGGAG GTCCATATCT 
GAGATCGAAG ACCACAACCG GCAGCTGACC CATGAGCTGA ACAAGTTCAA GTTTGAGCCT 
CGGCCGGAGC CAGGGTGGCT GGCGGATGGC GCGGGCAAGG GCGTGGGCAG CGGGGCCCCT 
CTGCAGGAGG AGCTCAAGTC GGCACGGCTA CAGATCAATG AGCTCAGTGG GAAGGTGCTG 
AAGCTGCAGT ATGAGAACCG GGTGCTACTG TCCAATGTGC AGCGCTATGA CCTGGCTGCC 
CACCTAGGCC TGCGTGCCCC CAGCCCCCGG GACAGCGACG CAGACAGCGA CCAGGGCAAG 
AAGGAGAGCG ATGGGGAGGA AGGCCGCCAG CCCCAGCCCA AGCGGGAGGG CCCTATTGGC 
GGGGAGAGCG ACTCAGAGGA GATGTGCGAG AAGACGTCGG GCTTCGGCAG CGGAAAGCCA 
TCGGAGGCGA GCGAGCCATG TCCCGCCGAG CTCCTGCGGG CCAGGGAGGA CACCGAGTGC 
CTGGCGAGCA TAAAGCACGA GGCTGAGCGC CTGGAGCGAA CAGTGGAGCG CCTCATCACG 
GACACCGCGG GCTTCGGGGA CGATGTGGGG CTGCTGGGCA GTGGCGAGCC CTTGCCAGGG 
CCCGACATCC AGGGGGCGGG GGGGACGGGC GAGGGGGGCA AGAAGGAGCC CCCACTGCTG 
GGGACCATCA ACTCGAGGAT GAAGGCTTTC AGGAAGGAGC TGCAGGCCTT CCTGGAGCAG 
GTGAACCGAA TCGGGGATGG CCTGCGGGAG CGCCTGGAAG GCCTGTCCCC CCTGCCTCAC 
CTCACAGAGT CTTCCAGCTT TCTCTCCACC GTCACTTCCG TGTCCCGGGA CTCCCCCGTC 
GGGAACCTGG GGAAGGAGCT GGTCCCGGAC CTGCAGTCCA AACTGAGAGG TCAGCTGGAG 
TGGCAGCCGG GGCAGGAGTG CGGGGACGAG CCAGAGCAGC TGCACCGCCG CGCTGATGGG 
GACGTCGGGA GCTGCCGGCA GGGAGGCCAG AGCTGCTTCA GTCTAGAGAG TGTATCGCTA 
GAGGAGCTGC AGGGTCAGCT TGCGCAGGCG ACCAGACTGC ATCAAGAGGA GACAGAGAAA 
TTTACAAACA AGATTCGGAA GATGGAGGAG GAGCACCTGT ACGCCTTGAG GTGGAAAGAA 
CTGGAAATGC ACAGCCTGGC TTTGCAGAAC ACCCTCCATG AGCAAACCTG GAGTGATGAG 
AGGAACCTGA TGCAGCAGGA GCTCCGGTCT ATGAAGCAGA ACATTTTCCT TTTCTACGTC 
AAACTCAGAT GGCTGCTGAG GCACTGGCGA CAAGGGAAGC AGATGGAGGA AGAAGGCGAG 
GATTTCACTG AGAGCGAGCA TCCAGAGACC CTCCCCAGGC TTGGTGAGCT TGGAGTGCAG 
GGGGACCCGA AGGGGGACGG CCCAGACCAA GATGACAACG GTCCGGGCTG CGGCTTTCCG 
CTGGGAGAGC ATCCTCCACA CTCCCCTGTG CAGATTGGTG ACCATGGATC GAGACTACAG 
CCTACACATG GGAGACAGCT CCACAGGCAG GTGGTGGAAA ACCAGCAGCT GTTCAGTGCA 
CTCAAGAGCC TGCTGGAGGA CCTGCGCGCC GAGCTGCGGG AGGACGAGCA TGCACGGCGG 
CGGCTGCAGC AGCAGTACGC CAGCGACAAA GCCGCCTGGG ACGTGGAGTG GGCTGCACTT 
ACCTGCCGCC TGGAACAGGT GCCTGCCTGC GGGGTCTGTC CTGGGTCTCC CCAGCTCCTC 
CTCTGCCATC TGGGCCAGCT GGAAGAGAAG ACTGAGAAGA ATTTGGGAGA AGCATGCTCT 
TCCACCGACA GCAAAGGGGC ATTCAAGAAG GAAAGAGAGA TGCATCAGAA GCTCCTCACT 
GACAGTCATG GCCTGGTCAT GGACCTGCGC TGGCAGATCC ATCACAGTGA GAAGAACTGG 
AGCCGGGAGA AGGTGGAACT TCTTAGCCGT CTAGACAGAG ACCGGCAGGA ATGGGAGCAG 
CAGAAGAAGG AGCTCTTGTG GAGAATAGAA CAGTTGCAGA AAGAAAACAG TCCCCGGAGA 
AGTGGCAGTT TCCTCTGTGA TCAAAAAGAA GGCACCATCC GCCCCTTTGC CCATCCAGGA 
AGCCCCCGAA TGCCCCGTCC CTTGGGGATG TGGTCCTGTG CAGACACCGA CTCCACCCCA 
TTCGAAGACC GGCCGCTGTC CAAGCTGAAG GAATCAGACA GATGTTCAGC ACGGGAAAAC 
CTCTACTTGG ACGCCTTGTC TCTAGATGAC GAGCCAGAGG AGCCTCCAGC CCGAGAGCCC 
CAGCGGGAGT TCAGGAACTG CCTCCCCCAG GAAGAAGAAA ATCACAAGGG AAATCTTCAA 
AGGGCCGTGT CTGTGTCCTC CATGTCTGAG TTCCAGCGTC TGATGGACGT TTCTCCCTTC 
CTGCCGGAGA AGGGCGTCCC CGCCACCAGC AGCAAGGAGG ACATCACTCC GCCCCTGTCT 
CCTGATGATC TGAAGTACAT CGAGGAGTTC AACAGCAAGA ACTGGGACGA GAGGCCCCCG 
GAGCCCTGGG CAGACAGGAC CGAGGGGGGG CGGGCAGGGA GCGAGGCTGG TGCAGAGCCT 
TTCCCTGACT CCTCCTGGTA CCTCACCACG AGTGTCACCA TGACTACGGA CACCATGACC 
AGCCCAGAGC ACTGCCAGAA GCAGCCCCTT CGGAGCCCCA TGTGCGCGGA GCAGGCCGGG 
GTGCGGGTGC TGCACAGCCC GCCCGCGGTC CGTAGGATGG ATGGCATCGT GGTGGCGGGC 
AGTGAGGGCA AGGGCCGGGC CGAGCCCGAG TGCCCGTTTC CAACTAGCAG AGCCAGGGGG 
GGCACTGGAG ACACAAAAGG GGGTCCCTCA GAACACGTGC TCAGCAGGTG GCCTTGCGCC 
CCCTCCAGGC ACCCCCGAGA CTATGTGGAG GGAGGGCTGC GCCCCCTCGA AAGTGCCCTC 
TGCGCCCCCC TGGGTTTTGC CTCCTCGCTG CACGGCCTGC AGATGTCCAA GAACATGAGT 
GATGACATGA AGGAGGTGGC CTTCTCGGTC AGGAACGCCA TCTGCTCTGG CCCCCCCAAG 
CCTCCGCTCA AGGACATGGC CTGCCAGACC AATGGGTCCC GGACGACGGG AACACAGACC 
GTCCAGACCA TCACCATTGG CCTGCAGACC GAAGCCCTGC GTGGCAGCGC CGTCACCAGC 
AGCCCCCACA AGTGTCTCAC ACCCAAGGCC GGGGGCGGGG CCACGCCCGC GTCATCTCCT 
TCCCGTGGCC TTCGGAGCCG GCAGATGGCC CCCGCCATCG AGAAGGTGCA GGCCAAGTTC 
GAACGCACGT GCTGCTCCCC CAAGTACGGG TCTCCCAAGC TGCAGAGGAA GCCCCTCCCC 
AAAGCTGAGC CGCCCAACAG CAGGACCTCC CTGGGGCTGG CCCCAAAGGG GTGCAGCGAG 
TCCGCCTGGG CCCGTTCCAC CACCACGAGG GAGAGCCCGG TGCACACCGC CATCAACGAT 
GGCCTCTCCA GCCTCTTCAA CATCATCGAC CACAGCCCCG TGGTGCAGGA CCCCTTCCAG 
AAGGGGACTC GGGCCGGGAG CCGATCTCGC TCAGCAGAAC CCCGGCCCGA GCTGGGCCCA 
GGCCAGGAAA CGGGCCCCAG TTCCCGGGGC CGGTCCCCCA GCCCCATCGG GGCTGGCTCA 
GAGACTTGGA GGGAGGAAGG GGGAGAGGGC ACGCCCTTGA GGCAGGACTT ATCCGCTCCC 
CCTGGTTACA CGTTCACTGA GAACGTAGCC CGGATCCTCA ACAAGAAGCT GTTGGAGCAT 
GCCTTAAAGG AGGAGAGGAG GCAGGCCCCC CACGGGCCCC CAAGTCTTAA CAGTGACAGC 
CACATGGGAG AAACCATCAA AGCTGAACCA GGGTCCATCG AGGAACTACC TTGTTCCGCA 
CTAGCTCCAT CCCTAGAACC CTGCTTCTCC AGGCCCGAGA GACCAGCAAA CCGTCGCCTT 
CCGTCCCGTT GGGCCTCACA TTCCCCCACT GCCTCACTGG CTCAGTCACC CGGAGACCCA 
GCCTCCTTGG AGGAGCTCGG CGATGAGGAG CCACCGGGGG AGAAGACACA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_022421631.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277339.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X16, mRNA.

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CloneID	OCe289890
Clone ID Related Accession (Same CDS sequence)	XM_022421631.1
Accession Version	XM_022421631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG AGTGTATCGC TAGAGGAGCT GCAGGGTCAG 
CTTGCGCAGG CGACCAGACT GCATCAAGAG GAGACAGAGA AATTTACAAA CAAGATTCGG 
AAGATGGAGG AGGAGCACCT GTACGCCTTG AGGTGGAAAG AACTGGAAAT GCACAGCCTG 
GCTTTGCAGA ACACCCTCCA TGAGCAAACC TGGAGTGATG AGAGGAACCT GATGCAGCAG 
GAGCTCCGGT CTATGAAGCA GAACATTTTC CTTTTCTACG TCAAACTCAG ATGGCTGCTG 
AGGCACTGGC GACAAGGGAA GCAGATGGAG GAAGAAGGCG AGGATTTCAC TGAGAGCGAG 
CATCCAGAGA CCCTCCCCAG GCTTGGTGAG CTTGGAGTGC AGGGGGACCC GAAGGGGGAC 
GGCCCAGACC AAGATGACAA CGGTCCGGGC TGCGGCTTTC CGCTGGGAGA GCATCCTCCA 
CACTCCCCTG TGCAGATTGG TGACCATGGA TCGAGACTAC AGCCTACACA TGGGAGACAG 
CTCCACAGGC AGGTGGTGGA AAACCAGCAG CTGTTCAGTG CACTCAAGAG CCTGCTGGAG 
GACCTGCGCG CCGAGCTGCG GGAGGACGAG CATGCACGGC GGCGGCTGCA GCAGCAGTAC 
GCCAGCGACA AAGCCGCCTG GGACGTGGAG TGGGCTGCAC TTACCTGCCG CCTGGAACAG 
CTGGAAGAGA AGACTGAGAA GAATTTGGGA GAAGCATGCT CTTCCACCGA CAGCAAAGGG 
GCATTCAAGA AGGAAAGAGA GATGCATCAG AAGCTCCTCA CTGACAGTCA TGGCCTGGTC 
ATGGACCTGC GCTGGCAGAT CCATCACAGT GAGAAGAACT GGAGCCGGGA GAAGGTGGAA 
CTTCTTAGCC GTCTAGACAG AGACCGGCAG GAATGGGAGC AGCAGAAGAA GGAGCTCTTG 
TGGAGAATAG AACAGTTGCA GAAAGAAAAC AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT 
GATCAAAAAG AAGGCACCAT CCGCCCCTTT GCCCATCCAG GAAGCCCCCG AATGCCCCGT 
CCCTTGGGGA TGTGGTCCTG TGCAGACACC GACTCCACCC CATTCGAAGA CCGGCCGCTG 
TCCAAGCTGA AGGAATCAGA CAGATGTTCA GCACGGGAAA ACCTCTACTT GGACGCCTTG 
TCTCTAGATG ACGAGCCAGA GGAGCCTCCA GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC 
TGCCTCCCCC AGGAAGAAGA AAATCACAAG GGAAATCTTC AAAGGGCCGT GTCTGTGTCC 
TCCATGTCTG AGTTCCAGCG TCTGATGGAC GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC 
CCCGCCACCA GCAGCAAGGA GGACATCACT CCGCCCCTGT CTCCTGATGA TCTGAAGTAC 
ATCGAGGAGT TCAACAGCAA GAACTGGGAC GAGAGGCCCC CGGAGCCCTG GGCAGACAGG 
ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG 
TACCTCACCA CGAGTGTCAC CATGACTACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCCC TTCGGAGCCC CATGTGCGCG GAGCAGGCCG GGGTGCGGGT GCTGCACAGC 
CCGCCCGCGG TCCGTAGGAT GGATGGCATC GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG 
GCCGAGCCCG AGTGCCCGTT TCCAACTAGC AGAGCCAGGG GGGGCACTGG AGACACAAAA 
GGGGGTCCCT CAGAACACGT GCTCAGCAGG TGGCCTTGCG CCCCCTCCAG GCACCCCCGA 
GACTATGTGG AGGGAGGGCT GCGCCCCCTC GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT 
GCCTCCTCGC TGCACGGCCT GCAGATGTCC AAGAACATGA GTGATGACAT GAAGGAGGTG 
GCCTTCTCGG TCAGGAACGC CATCTGCTCT GGCCCCCCCA AGCCTCCGCT CAAGGACATG 
GCCTGCCAGA CCAATGGGTC CCGGACGACG GGAACACAGA CCGTCCAGAC CATCACCATT 
GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC GCCGTCACCA GCAGCCCCCA CAAGTGTCTC 
ACACCCAAGG CCGGGGGCGG GGCCACGCCC GCGTCATCTC CTTCCCGTGG CCTTCGGAGC 
CGGCAGATGG CCCCCGCCAT CGAGAAGGTG CAGGCCAAGT TCGAACGCAC GTGCTGCTCC 
CCCAAGTACG GGTCTCCCAA GCTGCAGAGG AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC 
AGCAGGACCT CCCTGGGGCT GGCCCCAAAG GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC 
ACCACCACGA GGGAGAGCCC GGTGCACACC GCCATCAACG ATGGCCTCTC CAGCCTCTTC 
AACATCATCG ACCACAGCCC CGTGGTGCAG GACCCCTTCC AGAAGGGGAC TCGGGCCGGG 
AGCCGATCTC GCTCAGCAGA ACCCCGGCCC GAGCTGGGCC CAGGCCAGGA AACGGGCCCC 
AGTTCCCGGG GCCGGTCCCC CAGCCCCATC GGGGCTGGCT CAGAGACTTG GAGGGAGGAA 
GGGGGAGAGG GCACGCCCTT GAGGCAGGAC TTATCCGCTC CCCCTGGTTA CACGTTCACT 
GAGAACGTAG CCCGGATCCT CAACAAGAAG CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG 
AGGCAGGCCC CCCACGGGCC CCCAAGTCTT AACAGTGACA GCCACATGGG AGAAACCATC 
AAAGCTGAAC CAGGGTCCAT CGAGAACCAA ACTGTCTTAC TAACTGCCCC CTGGGAACTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277339.1
CDS	118..4920
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLESVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIENQTVLLTAPWEL

Target ORF information:

RefSeq Version	XM_022421631.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421631.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG AGTGTATCGC TAGAGGAGCT GCAGGGTCAG 
CTTGCGCAGG CGACCAGACT GCATCAAGAG GAGACAGAGA AATTTACAAA CAAGATTCGG 
AAGATGGAGG AGGAGCACCT GTACGCCTTG AGGTGGAAAG AACTGGAAAT GCACAGCCTG 
GCTTTGCAGA ACACCCTCCA TGAGCAAACC TGGAGTGATG AGAGGAACCT GATGCAGCAG 
GAGCTCCGGT CTATGAAGCA GAACATTTTC CTTTTCTACG TCAAACTCAG ATGGCTGCTG 
AGGCACTGGC GACAAGGGAA GCAGATGGAG GAAGAAGGCG AGGATTTCAC TGAGAGCGAG 
CATCCAGAGA CCCTCCCCAG GCTTGGTGAG CTTGGAGTGC AGGGGGACCC GAAGGGGGAC 
GGCCCAGACC AAGATGACAA CGGTCCGGGC TGCGGCTTTC CGCTGGGAGA GCATCCTCCA 
CACTCCCCTG TGCAGATTGG TGACCATGGA TCGAGACTAC AGCCTACACA TGGGAGACAG 
CTCCACAGGC AGGTGGTGGA AAACCAGCAG CTGTTCAGTG CACTCAAGAG CCTGCTGGAG 
GACCTGCGCG CCGAGCTGCG GGAGGACGAG CATGCACGGC GGCGGCTGCA GCAGCAGTAC 
GCCAGCGACA AAGCCGCCTG GGACGTGGAG TGGGCTGCAC TTACCTGCCG CCTGGAACAG 
CTGGAAGAGA AGACTGAGAA GAATTTGGGA GAAGCATGCT CTTCCACCGA CAGCAAAGGG 
GCATTCAAGA AGGAAAGAGA GATGCATCAG AAGCTCCTCA CTGACAGTCA TGGCCTGGTC 
ATGGACCTGC GCTGGCAGAT CCATCACAGT GAGAAGAACT GGAGCCGGGA GAAGGTGGAA 
CTTCTTAGCC GTCTAGACAG AGACCGGCAG GAATGGGAGC AGCAGAAGAA GGAGCTCTTG 
TGGAGAATAG AACAGTTGCA GAAAGAAAAC AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT 
GATCAAAAAG AAGGCACCAT CCGCCCCTTT GCCCATCCAG GAAGCCCCCG AATGCCCCGT 
CCCTTGGGGA TGTGGTCCTG TGCAGACACC GACTCCACCC CATTCGAAGA CCGGCCGCTG 
TCCAAGCTGA AGGAATCAGA CAGATGTTCA GCACGGGAAA ACCTCTACTT GGACGCCTTG 
TCTCTAGATG ACGAGCCAGA GGAGCCTCCA GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC 
TGCCTCCCCC AGGAAGAAGA AAATCACAAG GGAAATCTTC AAAGGGCCGT GTCTGTGTCC 
TCCATGTCTG AGTTCCAGCG TCTGATGGAC GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC 
CCCGCCACCA GCAGCAAGGA GGACATCACT CCGCCCCTGT CTCCTGATGA TCTGAAGTAC 
ATCGAGGAGT TCAACAGCAA GAACTGGGAC GAGAGGCCCC CGGAGCCCTG GGCAGACAGG 
ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG 
TACCTCACCA CGAGTGTCAC CATGACTACG GACACCATGA CCAGCCCAGA GCACTGCCAG 
AAGCAGCCCC TTCGGAGCCC CATGTGCGCG GAGCAGGCCG GGGTGCGGGT GCTGCACAGC 
CCGCCCGCGG TCCGTAGGAT GGATGGCATC GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG 
GCCGAGCCCG AGTGCCCGTT TCCAACTAGC AGAGCCAGGG GGGGCACTGG AGACACAAAA 
GGGGGTCCCT CAGAACACGT GCTCAGCAGG TGGCCTTGCG CCCCCTCCAG GCACCCCCGA 
GACTATGTGG AGGGAGGGCT GCGCCCCCTC GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT 
GCCTCCTCGC TGCACGGCCT GCAGATGTCC AAGAACATGA GTGATGACAT GAAGGAGGTG 
GCCTTCTCGG TCAGGAACGC CATCTGCTCT GGCCCCCCCA AGCCTCCGCT CAAGGACATG 
GCCTGCCAGA CCAATGGGTC CCGGACGACG GGAACACAGA CCGTCCAGAC CATCACCATT 
GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC GCCGTCACCA GCAGCCCCCA CAAGTGTCTC 
ACACCCAAGG CCGGGGGCGG GGCCACGCCC GCGTCATCTC CTTCCCGTGG CCTTCGGAGC 
CGGCAGATGG CCCCCGCCAT CGAGAAGGTG CAGGCCAAGT TCGAACGCAC GTGCTGCTCC 
CCCAAGTACG GGTCTCCCAA GCTGCAGAGG AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC 
AGCAGGACCT CCCTGGGGCT GGCCCCAAAG GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC 
ACCACCACGA GGGAGAGCCC GGTGCACACC GCCATCAACG ATGGCCTCTC CAGCCTCTTC 
AACATCATCG ACCACAGCCC CGTGGTGCAG GACCCCTTCC AGAAGGGGAC TCGGGCCGGG 
AGCCGATCTC GCTCAGCAGA ACCCCGGCCC GAGCTGGGCC CAGGCCAGGA AACGGGCCCC 
AGTTCCCGGG GCCGGTCCCC CAGCCCCATC GGGGCTGGCT CAGAGACTTG GAGGGAGGAA 
GGGGGAGAGG GCACGCCCTT GAGGCAGGAC TTATCCGCTC CCCCTGGTTA CACGTTCACT 
GAGAACGTAG CCCGGATCCT CAACAAGAAG CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG 
AGGCAGGCCC CCCACGGGCC CCCAAGTCTT AACAGTGACA GCCACATGGG AGAAACCATC 
AAAGCTGAAC CAGGGTCCAT CGAGAACCAA ACTGTCTTAC TAACTGCCCC CTGGGAACTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005623075.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005623132.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe09960
Clone ID Related Accession (Same CDS sequence)	XM_005623075.1
Accession Version	XM_005623075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	microtubule cross-linking factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG CTCAGGGGCC CCCCTGTTTT ACCTGAACAG 
AGTGTATCGC TAGAGGAGCT GCAGGGTCAG CTTGCGCAGG CGACCAGACT GCATCAAGAG 
GAGACAGAGA AATTTACAAA CAAGATTCGG AAGATGGAGG AGGAGCACCT GTACGCCTTG 
AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAGA ACACCCTCCA TGAGCAAACC 
TGGAGTGATG AGAGGAACCT GATGCAGCAG GAGCTCCGGT CTATGAAGCA GAACATTTTC 
CTTTTCTACG TCAAACTCAG ATGGCTGCTG AGGCACTGGC GACAAGGGAA GCAGATGGAG 
GAAGAAGGCG AGGATTTCAC TGAGAGCGAG CATCCAGAGA CCCTCCCCAG GCTTGGTGAG 
CTTGGAGTGC AGGGGGACCC GAAGGGGGAC GGCCCAGACC AAGATGACAA CGGTCCGGGC 
TGCGGCTTTC CGCTGGGAGA GCATCCTCCA CACTCCCCTG TGCAGATTGG TGACCATGGA 
TCGAGACTAC AGCCTACACA TGGGAGACAG CTCCACAGGC AGGTGGTGGA AAACCAGCAG 
CTGTTCAGTG CACTCAAGAG CCTGCTGGAG GACCTGCGCG CCGAGCTGCG GGAGGACGAG 
CATGCACGGC GGCGGCTGCA GCAGCAGTAC GCCAGCGACA AAGCCGCCTG GGACGTGGAG 
TGGGCTGCAC TTACCTGCCG CCTGGAACAG CTGGAAGAGA AGACTGAGAA GAATTTGGGA 
GAAGCATGCT CTTCCACCGA CAGCAAAGGG GCATTCAAGA AGGAAAGAGA GATGCATCAG 
AAGCTCCTCA CTGACAGTCA TGGCCTGGTC ATGGACCTGC GCTGGCAGAT CCATCACAGT 
GAGAAGAACT GGAGCCGGGA GAAGGTGGAA CTTCTTAGCC GTCTAGACAG AGACCGGCAG 
GAATGGGAGC AGCAGAAGAA GGAGCTCTTG TGGAGAATAG AACAGTTGCA GAAAGAAAAC 
AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT GATCAAAAAG AAGGCACCAT CCGCCCCTTT 
GCCCATCCAG GAAGCCCCCG AATGCCCCGT CCCTTGGGGA TGTGGTCCTG TGCAGACACC 
GACTCCACCC CATTCGAAGA CCGGCCGCTG TCCAAGCTGA AGGAATCAGA CAGATGTTCA 
GCACGGGAAA ACCTCTACTT GGACGCCTTG TCTCTAGATG ACGAGCCAGA GGAGCCTCCA 
GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC TGCCTCCCCC AGGAAGAAGA AAATCACAAG 
GGAAATCTTC AAAGGGCCGT GTCTGTGTCC TCCATGTCTG AGTTCCAGCG TCTGATGGAC 
GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC CCCGCCACCA GCAGCAAGGA GGACATCACT 
CCGCCCCTGT CTCCTGATGA TCTGAAGTAC ATCGAGGAGT TCAACAGCAA GAACTGGGAC 
GAGAGGCCCC CGGAGCCCTG GGCAGACAGG ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT 
GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG TACCTCACCA CGAGTGTCAC CATGACTACG 
GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCCC TTCGGAGCCC CATGTGCGCG 
GAGCAGGCCG GGGTGCGGGT GCTGCACAGC CCGCCCGCGG TCCGTAGGAT GGATGGCATC 
GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG GCCGAGCCCG AGTGCCCGTT TCCAACTAGC 
AGAGCCAGGG GGGGCACTGG AGACACAAAA GGGGGTCCCT CAGAACACGT GCTCAGCAGG 
TGGCCTTGCG CCCCCTCCAG GCACCCCCGA GACTATGTGG AGGGAGGGCT GCGCCCCCTC 
GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT GCCTCCTCGC TGCACGGCCT GCAGATGTCC 
AAGAACATGA GTGATGACAT GAAGGAGGTG GCCTTCTCGG TCAGGAACGC CATCTGCTCT 
GGCCCCCCCA AGCCTCCGCT CAAGGACATG GCCTGCCAGA CCAATGGGTC CCGGACGACG 
GGAACACAGA CCGTCCAGAC CATCACCATT GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC 
GCCGTCACCA GCAGCCCCCA CAAGTGTCTC ACACCCAAGG CCGGGGGCGG GGCCACGCCC 
GCGTCATCTC CTTCCCGTGG CCTTCGGAGC CGGCAGATGG CCCCCGCCAT CGAGAAGGTG 
CAGGCCAAGT TCGAACGCAC GTGCTGCTCC CCCAAGTACG GGTCTCCCAA GCTGCAGAGG 
AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC AGCAGGACCT CCCTGGGGCT GGCCCCAAAG 
GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC ACCACCACGA GGGAGAGCCC GGTGCACACC 
GCCATCAACG ATGGCCTCTC CAGCCTCTTC AACATCATCG ACCACAGCCC CGTGGTGCAG 
GACCCCTTCC AGAAGGGGAC TCGGGCCGGG AGCCGATCTC GCTCAGCAGA ACCCCGGCCC 
GAGCTGGGCC CAGGCCAGGA AACGGGCCCC AGTTCCCGGG GCCGGTCCCC CAGCCCCATC 
GGGGCTGGCT CAGAGACTTG GAGGGAGGAA GGGGGAGAGG GCACGCCCTT GAGGCAGGAC 
TTATCCGCTC CCCCTGGTTA CACGTTCACT GAGAACGTAG CCCGGATCCT CAACAAGAAG 
CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AGGCAGGCCC CCCACGGGCC CCCAAGTCTT 
AACAGTGACA GCCACATGGG AGAAACCATC AAAGCTGAAC CAGGGTCCAT CGAGGAACTA 
CCTTGTTCCG CACTAGCTCC ATCCCTAGAA CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC TTCCGTCCCG TTGGGCCTCA CATTCCCCCA CTGCCTCACT GGCTCAGTCA 
CCCGGAGACC CAGCCTCCTT GGAGGAGCTC GGCGATGAGG AGCCACCGGG GGAGAAGACA 
CACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623132.1
CDS	80..5068
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLELRGPPVLPEQSVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIEELPCSALAPSLEPCFSRPERPANRRLPSRWASHSPTASLAQSPGDPASLEELGDEEPPGEKTHL

Target ORF information:

RefSeq Version	XM_005623075.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623075.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG CTCAGGGGCC CCCCTGTTTT ACCTGAACAG 
AGTGTATCGC TAGAGGAGCT GCAGGGTCAG CTTGCGCAGG CGACCAGACT GCATCAAGAG 
GAGACAGAGA AATTTACAAA CAAGATTCGG AAGATGGAGG AGGAGCACCT GTACGCCTTG 
AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAGA ACACCCTCCA TGAGCAAACC 
TGGAGTGATG AGAGGAACCT GATGCAGCAG GAGCTCCGGT CTATGAAGCA GAACATTTTC 
CTTTTCTACG TCAAACTCAG ATGGCTGCTG AGGCACTGGC GACAAGGGAA GCAGATGGAG 
GAAGAAGGCG AGGATTTCAC TGAGAGCGAG CATCCAGAGA CCCTCCCCAG GCTTGGTGAG 
CTTGGAGTGC AGGGGGACCC GAAGGGGGAC GGCCCAGACC AAGATGACAA CGGTCCGGGC 
TGCGGCTTTC CGCTGGGAGA GCATCCTCCA CACTCCCCTG TGCAGATTGG TGACCATGGA 
TCGAGACTAC AGCCTACACA TGGGAGACAG CTCCACAGGC AGGTGGTGGA AAACCAGCAG 
CTGTTCAGTG CACTCAAGAG CCTGCTGGAG GACCTGCGCG CCGAGCTGCG GGAGGACGAG 
CATGCACGGC GGCGGCTGCA GCAGCAGTAC GCCAGCGACA AAGCCGCCTG GGACGTGGAG 
TGGGCTGCAC TTACCTGCCG CCTGGAACAG CTGGAAGAGA AGACTGAGAA GAATTTGGGA 
GAAGCATGCT CTTCCACCGA CAGCAAAGGG GCATTCAAGA AGGAAAGAGA GATGCATCAG 
AAGCTCCTCA CTGACAGTCA TGGCCTGGTC ATGGACCTGC GCTGGCAGAT CCATCACAGT 
GAGAAGAACT GGAGCCGGGA GAAGGTGGAA CTTCTTAGCC GTCTAGACAG AGACCGGCAG 
GAATGGGAGC AGCAGAAGAA GGAGCTCTTG TGGAGAATAG AACAGTTGCA GAAAGAAAAC 
AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT GATCAAAAAG AAGGCACCAT CCGCCCCTTT 
GCCCATCCAG GAAGCCCCCG AATGCCCCGT CCCTTGGGGA TGTGGTCCTG TGCAGACACC 
GACTCCACCC CATTCGAAGA CCGGCCGCTG TCCAAGCTGA AGGAATCAGA CAGATGTTCA 
GCACGGGAAA ACCTCTACTT GGACGCCTTG TCTCTAGATG ACGAGCCAGA GGAGCCTCCA 
GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC TGCCTCCCCC AGGAAGAAGA AAATCACAAG 
GGAAATCTTC AAAGGGCCGT GTCTGTGTCC TCCATGTCTG AGTTCCAGCG TCTGATGGAC 
GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC CCCGCCACCA GCAGCAAGGA GGACATCACT 
CCGCCCCTGT CTCCTGATGA TCTGAAGTAC ATCGAGGAGT TCAACAGCAA GAACTGGGAC 
GAGAGGCCCC CGGAGCCCTG GGCAGACAGG ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT 
GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG TACCTCACCA CGAGTGTCAC CATGACTACG 
GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCCC TTCGGAGCCC CATGTGCGCG 
GAGCAGGCCG GGGTGCGGGT GCTGCACAGC CCGCCCGCGG TCCGTAGGAT GGATGGCATC 
GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG GCCGAGCCCG AGTGCCCGTT TCCAACTAGC 
AGAGCCAGGG GGGGCACTGG AGACACAAAA GGGGGTCCCT CAGAACACGT GCTCAGCAGG 
TGGCCTTGCG CCCCCTCCAG GCACCCCCGA GACTATGTGG AGGGAGGGCT GCGCCCCCTC 
GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT GCCTCCTCGC TGCACGGCCT GCAGATGTCC 
AAGAACATGA GTGATGACAT GAAGGAGGTG GCCTTCTCGG TCAGGAACGC CATCTGCTCT 
GGCCCCCCCA AGCCTCCGCT CAAGGACATG GCCTGCCAGA CCAATGGGTC CCGGACGACG 
GGAACACAGA CCGTCCAGAC CATCACCATT GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC 
GCCGTCACCA GCAGCCCCCA CAAGTGTCTC ACACCCAAGG CCGGGGGCGG GGCCACGCCC 
GCGTCATCTC CTTCCCGTGG CCTTCGGAGC CGGCAGATGG CCCCCGCCAT CGAGAAGGTG 
CAGGCCAAGT TCGAACGCAC GTGCTGCTCC CCCAAGTACG GGTCTCCCAA GCTGCAGAGG 
AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC AGCAGGACCT CCCTGGGGCT GGCCCCAAAG 
GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC ACCACCACGA GGGAGAGCCC GGTGCACACC 
GCCATCAACG ATGGCCTCTC CAGCCTCTTC AACATCATCG ACCACAGCCC CGTGGTGCAG 
GACCCCTTCC AGAAGGGGAC TCGGGCCGGG AGCCGATCTC GCTCAGCAGA ACCCCGGCCC 
GAGCTGGGCC CAGGCCAGGA AACGGGCCCC AGTTCCCGGG GCCGGTCCCC CAGCCCCATC 
GGGGCTGGCT CAGAGACTTG GAGGGAGGAA GGGGGAGAGG GCACGCCCTT GAGGCAGGAC 
TTATCCGCTC CCCCTGGTTA CACGTTCACT GAGAACGTAG CCCGGATCCT CAACAAGAAG 
CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AGGCAGGCCC CCCACGGGCC CCCAAGTCTT 
AACAGTGACA GCCACATGGG AGAAACCATC AAAGCTGAAC CAGGGTCCAT CGAGGAACTA 
CCTTGTTCCG CACTAGCTCC ATCCCTAGAA CCCTGCTTCT CCAGGCCCGA GAGACCAGCA 
AACCGTCGCC TTCCGTCCCG TTGGGCCTCA CATTCCCCCA CTGCCTCACT GGCTCAGTCA 
CCCGGAGACC CAGCCTCCTT GGAGGAGCTC GGCGATGAGG AGCCACCGGG GGAGAAGACA 
CACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005623078.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005623135.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe09963
Clone ID Related Accession (Same CDS sequence)	XM_005623078.1
Accession Version	XM_005623078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	microtubule cross-linking factor 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG CTCAGGGGCC CCCCTGTTTT ACCTGAACAG 
AGTGTATCGC TAGAGGAGCT GCAGGGTCAG CTTGCGCAGG CGACCAGACT GCATCAAGAG 
GAGACAGAGA AATTTACAAA CAAGATTCGG AAGATGGAGG AGGAGCACCT GTACGCCTTG 
AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAGA ACACCCTCCA TGAGCAAACC 
TGGAGTGATG AGAGGAACCT GATGCAGCAG GAGCTCCGGT CTATGAAGCA GAACATTTTC 
CTTTTCTACG TCAAACTCAG ATGGCTGCTG AGGCACTGGC GACAAGGGAA GCAGATGGAG 
GAAGAAGGCG AGGATTTCAC TGAGAGCGAG CATCCAGAGA CCCTCCCCAG GCTTGGTGAG 
CTTGGAGTGC AGGGGGACCC GAAGGGGGAC GGCCCAGACC AAGATGACAA CGGTCCGGGC 
TGCGGCTTTC CGCTGGGAGA GCATCCTCCA CACTCCCCTG TGCAGATTGG TGACCATGGA 
TCGAGACTAC AGCCTACACA TGGGAGACAG CTCCACAGGC AGGTGGTGGA AAACCAGCAG 
CTGTTCAGTG CACTCAAGAG CCTGCTGGAG GACCTGCGCG CCGAGCTGCG GGAGGACGAG 
CATGCACGGC GGCGGCTGCA GCAGCAGTAC GCCAGCGACA AAGCCGCCTG GGACGTGGAG 
TGGGCTGCAC TTACCTGCCG CCTGGAACAG CTGGAAGAGA AGACTGAGAA GAATTTGGGA 
GAAGCATGCT CTTCCACCGA CAGCAAAGGG GCATTCAAGA AGGAAAGAGA GATGCATCAG 
AAGCTCCTCA CTGACAGTCA TGGCCTGGTC ATGGACCTGC GCTGGCAGAT CCATCACAGT 
GAGAAGAACT GGAGCCGGGA GAAGGTGGAA CTTCTTAGCC GTCTAGACAG AGACCGGCAG 
GAATGGGAGC AGCAGAAGAA GGAGCTCTTG TGGAGAATAG AACAGTTGCA GAAAGAAAAC 
AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT GATCAAAAAG AAGGCACCAT CCGCCCCTTT 
GCCCATCCAG GAAGCCCCCG AATGCCCCGT CCCTTGGGGA TGTGGTCCTG TGCAGACACC 
GACTCCACCC CATTCGAAGA CCGGCCGCTG TCCAAGCTGA AGGAATCAGA CAGATGTTCA 
GCACGGGAAA ACCTCTACTT GGACGCCTTG TCTCTAGATG ACGAGCCAGA GGAGCCTCCA 
GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC TGCCTCCCCC AGGAAGAAGA AAATCACAAG 
GGAAATCTTC AAAGGGCCGT GTCTGTGTCC TCCATGTCTG AGTTCCAGCG TCTGATGGAC 
GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC CCCGCCACCA GCAGCAAGGA GGACATCACT 
CCGCCCCTGT CTCCTGATGA TCTGAAGTAC ATCGAGGAGT TCAACAGCAA GAACTGGGAC 
GAGAGGCCCC CGGAGCCCTG GGCAGACAGG ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT 
GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG TACCTCACCA CGAGTGTCAC CATGACTACG 
GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCCC TTCGGAGCCC CATGTGCGCG 
GAGCAGGCCG GGGTGCGGGT GCTGCACAGC CCGCCCGCGG TCCGTAGGAT GGATGGCATC 
GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG GCCGAGCCCG AGTGCCCGTT TCCAACTAGC 
AGAGCCAGGG GGGGCACTGG AGACACAAAA GGGGGTCCCT CAGAACACGT GCTCAGCAGG 
TGGCCTTGCG CCCCCTCCAG GCACCCCCGA GACTATGTGG AGGGAGGGCT GCGCCCCCTC 
GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT GCCTCCTCGC TGCACGGCCT GCAGATGTCC 
AAGAACATGA GTGATGACAT GAAGGAGGTG GCCTTCTCGG TCAGGAACGC CATCTGCTCT 
GGCCCCCCCA AGCCTCCGCT CAAGGACATG GCCTGCCAGA CCAATGGGTC CCGGACGACG 
GGAACACAGA CCGTCCAGAC CATCACCATT GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC 
GCCGTCACCA GCAGCCCCCA CAAGTGTCTC ACACCCAAGG CCGGGGGCGG GGCCACGCCC 
GCGTCATCTC CTTCCCGTGG CCTTCGGAGC CGGCAGATGG CCCCCGCCAT CGAGAAGGTG 
CAGGCCAAGT TCGAACGCAC GTGCTGCTCC CCCAAGTACG GGTCTCCCAA GCTGCAGAGG 
AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC AGCAGGACCT CCCTGGGGCT GGCCCCAAAG 
GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC ACCACCACGA GGGAGAGCCC GGTGCACACC 
GCCATCAACG ATGGCCTCTC CAGCCTCTTC AACATCATCG ACCACAGCCC CGTGGTGCAG 
GACCCCTTCC AGAAGGGGAC TCGGGCCGGG AGCCGATCTC GCTCAGCAGA ACCCCGGCCC 
GAGCTGGGCC CAGGCCAGGA AACGGGCCCC AGTTCCCGGG GCCGGTCCCC CAGCCCCATC 
GGGGCTGGCT CAGAGACTTG GAGGGAGGAA GGGGGAGAGG GCACGCCCTT GAGGCAGGAC 
TTATCCGCTC CCCCTGGTTA CACGTTCACT GAGAACGTAG CCCGGATCCT CAACAAGAAG 
CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AGGCAGGCCC CCCACGGGCC CCCAAGTCTT 
AACAGTGACA GCCACATGGG AGAAACCATC AAAGCTGAAC CAGGGTCCAT CGAGAACCAA 
ACTGTCTTAC TAACTGCCCC CTGGGAACTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623135.1
CDS	80..4912
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLELRGPPVLPEQSVSLEELQGQLAQATRLHQEETEKFTNKIRKMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIENQTVLLTAPWEL

Target ORF information:

RefSeq Version	XM_005623078.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623078.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG CTCAGGGGCC CCCCTGTTTT ACCTGAACAG 
AGTGTATCGC TAGAGGAGCT GCAGGGTCAG CTTGCGCAGG CGACCAGACT GCATCAAGAG 
GAGACAGAGA AATTTACAAA CAAGATTCGG AAGATGGAGG AGGAGCACCT GTACGCCTTG 
AGGTGGAAAG AACTGGAAAT GCACAGCCTG GCTTTGCAGA ACACCCTCCA TGAGCAAACC 
TGGAGTGATG AGAGGAACCT GATGCAGCAG GAGCTCCGGT CTATGAAGCA GAACATTTTC 
CTTTTCTACG TCAAACTCAG ATGGCTGCTG AGGCACTGGC GACAAGGGAA GCAGATGGAG 
GAAGAAGGCG AGGATTTCAC TGAGAGCGAG CATCCAGAGA CCCTCCCCAG GCTTGGTGAG 
CTTGGAGTGC AGGGGGACCC GAAGGGGGAC GGCCCAGACC AAGATGACAA CGGTCCGGGC 
TGCGGCTTTC CGCTGGGAGA GCATCCTCCA CACTCCCCTG TGCAGATTGG TGACCATGGA 
TCGAGACTAC AGCCTACACA TGGGAGACAG CTCCACAGGC AGGTGGTGGA AAACCAGCAG 
CTGTTCAGTG CACTCAAGAG CCTGCTGGAG GACCTGCGCG CCGAGCTGCG GGAGGACGAG 
CATGCACGGC GGCGGCTGCA GCAGCAGTAC GCCAGCGACA AAGCCGCCTG GGACGTGGAG 
TGGGCTGCAC TTACCTGCCG CCTGGAACAG CTGGAAGAGA AGACTGAGAA GAATTTGGGA 
GAAGCATGCT CTTCCACCGA CAGCAAAGGG GCATTCAAGA AGGAAAGAGA GATGCATCAG 
AAGCTCCTCA CTGACAGTCA TGGCCTGGTC ATGGACCTGC GCTGGCAGAT CCATCACAGT 
GAGAAGAACT GGAGCCGGGA GAAGGTGGAA CTTCTTAGCC GTCTAGACAG AGACCGGCAG 
GAATGGGAGC AGCAGAAGAA GGAGCTCTTG TGGAGAATAG AACAGTTGCA GAAAGAAAAC 
AGTCCCCGGA GAAGTGGCAG TTTCCTCTGT GATCAAAAAG AAGGCACCAT CCGCCCCTTT 
GCCCATCCAG GAAGCCCCCG AATGCCCCGT CCCTTGGGGA TGTGGTCCTG TGCAGACACC 
GACTCCACCC CATTCGAAGA CCGGCCGCTG TCCAAGCTGA AGGAATCAGA CAGATGTTCA 
GCACGGGAAA ACCTCTACTT GGACGCCTTG TCTCTAGATG ACGAGCCAGA GGAGCCTCCA 
GCCCGAGAGC CCCAGCGGGA GTTCAGGAAC TGCCTCCCCC AGGAAGAAGA AAATCACAAG 
GGAAATCTTC AAAGGGCCGT GTCTGTGTCC TCCATGTCTG AGTTCCAGCG TCTGATGGAC 
GTTTCTCCCT TCCTGCCGGA GAAGGGCGTC CCCGCCACCA GCAGCAAGGA GGACATCACT 
CCGCCCCTGT CTCCTGATGA TCTGAAGTAC ATCGAGGAGT TCAACAGCAA GAACTGGGAC 
GAGAGGCCCC CGGAGCCCTG GGCAGACAGG ACCGAGGGGG GGCGGGCAGG GAGCGAGGCT 
GGTGCAGAGC CTTTCCCTGA CTCCTCCTGG TACCTCACCA CGAGTGTCAC CATGACTACG 
GACACCATGA CCAGCCCAGA GCACTGCCAG AAGCAGCCCC TTCGGAGCCC CATGTGCGCG 
GAGCAGGCCG GGGTGCGGGT GCTGCACAGC CCGCCCGCGG TCCGTAGGAT GGATGGCATC 
GTGGTGGCGG GCAGTGAGGG CAAGGGCCGG GCCGAGCCCG AGTGCCCGTT TCCAACTAGC 
AGAGCCAGGG GGGGCACTGG AGACACAAAA GGGGGTCCCT CAGAACACGT GCTCAGCAGG 
TGGCCTTGCG CCCCCTCCAG GCACCCCCGA GACTATGTGG AGGGAGGGCT GCGCCCCCTC 
GAAAGTGCCC TCTGCGCCCC CCTGGGTTTT GCCTCCTCGC TGCACGGCCT GCAGATGTCC 
AAGAACATGA GTGATGACAT GAAGGAGGTG GCCTTCTCGG TCAGGAACGC CATCTGCTCT 
GGCCCCCCCA AGCCTCCGCT CAAGGACATG GCCTGCCAGA CCAATGGGTC CCGGACGACG 
GGAACACAGA CCGTCCAGAC CATCACCATT GGCCTGCAGA CCGAAGCCCT GCGTGGCAGC 
GCCGTCACCA GCAGCCCCCA CAAGTGTCTC ACACCCAAGG CCGGGGGCGG GGCCACGCCC 
GCGTCATCTC CTTCCCGTGG CCTTCGGAGC CGGCAGATGG CCCCCGCCAT CGAGAAGGTG 
CAGGCCAAGT TCGAACGCAC GTGCTGCTCC CCCAAGTACG GGTCTCCCAA GCTGCAGAGG 
AAGCCCCTCC CCAAAGCTGA GCCGCCCAAC AGCAGGACCT CCCTGGGGCT GGCCCCAAAG 
GGGTGCAGCG AGTCCGCCTG GGCCCGTTCC ACCACCACGA GGGAGAGCCC GGTGCACACC 
GCCATCAACG ATGGCCTCTC CAGCCTCTTC AACATCATCG ACCACAGCCC CGTGGTGCAG 
GACCCCTTCC AGAAGGGGAC TCGGGCCGGG AGCCGATCTC GCTCAGCAGA ACCCCGGCCC 
GAGCTGGGCC CAGGCCAGGA AACGGGCCCC AGTTCCCGGG GCCGGTCCCC CAGCCCCATC 
GGGGCTGGCT CAGAGACTTG GAGGGAGGAA GGGGGAGAGG GCACGCCCTT GAGGCAGGAC 
TTATCCGCTC CCCCTGGTTA CACGTTCACT GAGAACGTAG CCCGGATCCT CAACAAGAAG 
CTGTTGGAGC ATGCCTTAAA GGAGGAGAGG AGGCAGGCCC CCCACGGGCC CCCAAGTCTT 
AACAGTGACA GCCACATGGG AGAAACCATC AAAGCTGAAC CAGGGTCCAT CGAGAACCAA 
ACTGTCTTAC TAACTGCCCC CTGGGAACTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005623080.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005623137.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

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CloneID	OCe09965
Clone ID Related Accession (Same CDS sequence)	XM_005623080.2 , XM_005623080.3
Accession Version	XM_005623080.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	microtubule cross-linking factor 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG 
AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT 
TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA 
GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT 
GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC 
GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG 
AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT 
GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG 
GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA 
GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG 
CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG 
AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA 
TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT 
CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC 
CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC 
TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA 
CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC 
CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA 
AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT 
TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG 
CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG 
AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT 
GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC 
ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG 
CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG 
GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA 
GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG 
CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA 
AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG 
AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC 
CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA 
ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC 
GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG 
TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG 
GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG 
CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG 
TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC 
ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC 
CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG 
CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG 
GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA 
TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG 
TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC 
AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GAACCAAACT 
GTCTTACTAA CTGCCCCCTG GGAACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623137.1
CDS	365..5074
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLEMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIENQTVLLTAPWEL

Target ORF information:

RefSeq Version	XM_005623080.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623080.2

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG 
AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT 
TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA 
GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT 
GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC 
GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG 
AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT 
GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG 
GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA 
GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG 
CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG 
AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA 
TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT 
CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC 
CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC 
TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA 
CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC 
CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA 
AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT 
TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG 
CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG 
AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT 
GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC 
ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG 
CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG 
GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA 
GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG 
CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA 
AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG 
AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC 
CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA 
ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC 
GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG 
TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG 
GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG 
CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG 
TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC 
ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC 
CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG 
CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG 
GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA 
TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG 
TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC 
AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GAACCAAACT 
GTCTTACTAA CTGCCCCCTG GGAACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005623080.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005623137.1 Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X15, mRNA.

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CloneID	OCe09965
Clone ID Related Accession (Same CDS sequence)	XM_005623080.2 , XM_005623080.3
Accession Version	XM_005623080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	microtubule cross-linking factor 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG 
AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT 
TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA 
GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT 
GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC 
GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG 
AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT 
GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG 
GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA 
GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG 
CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG 
AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA 
TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT 
CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC 
CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC 
TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA 
CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC 
CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA 
AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT 
TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG 
CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG 
AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT 
GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC 
ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG 
CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG 
GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA 
GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG 
CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA 
AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG 
AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC 
CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA 
ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC 
GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG 
TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG 
GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG 
CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG 
TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC 
ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC 
CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG 
CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG 
GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA 
TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG 
TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC 
AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GAACCAAACT 
GTCTTACTAA CTGCCCCCTG GGAACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623137.1
CDS	132..4841
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRAHKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENESLRQQMIEVEISKQALQNELERLKESSLKRRGNRETYKEKKTFNQDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDMRLQYEREGTGRDHITGIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELNKFKFEPRPEPGWLADGAGKGVGSGAPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLRAPSPRDSDADSDQGKKESDGEEGRQPQPKREGPIGGESDSEEMCEKTSGFGSGKPSEASEPCPAELLRAREDTECLASIKHEAERLERTVERLITDTAGFGDDVGLLGSGEPLPGPDIQGAGGTGEGGKKEPPLLGTINSRMKAFRKELQAFLEQVNRIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPVGNLGKELVPDLQSKLRGQLEWQPGQECGDEPEQLHRRADGDVGSCRQGGQSCFSLEMEEEHLYALRWKELEMHSLALQNTLHEQTWSDERNLMQQELRSMKQNIFLFYVKLRWLLRHWRQGKQMEEEGEDFTESEHPETLPRLGELGVQGDPKGDGPDQDDNGPGCGFPLGEHPPHSPVQIGDHGSRLQPTHGRQLHRQVVENQQLFSALKSLLEDLRAELREDEHARRRLQQQYASDKAAWDVEWAALTCRLEQLEEKTEKNLGEACSSTDSKGAFKKEREMHQKLLTDSHGLVMDLRWQIHHSEKNWSREKVELLSRLDRDRQEWEQQKKELLWRIEQLQKENSPRRSGSFLCDQKEGTIRPFAHPGSPRMPRPLGMWSCADTDSTPFEDRPLSKLKESDRCSARENLYLDALSLDDEPEEPPAREPQREFRNCLPQEEENHKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGVPATSSKEDITPPLSPDDLKYIEEFNSKNWDERPPEPWADRTEGGRAGSEAGAEPFPDSSWYLTTSVTMTTDTMTSPEHCQKQPLRSPMCAEQAGVRVLHSPPAVRRMDGIVVAGSEGKGRAEPECPFPTSRARGGTGDTKGGPSEHVLSRWPCAPSRHPRDYVEGGLRPLESALCAPLGFASSLHGLQMSKNMSDDMKEVAFSVRNAICSGPPKPPLKDMACQTNGSRTTGTQTVQTITIGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQMAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKAEPPNSRTSLGLAPKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGTRAGSRSRSAEPRPELGPGQETGPSSRGRSPSPIGAGSETWREEGGEGTPLRQDLSAPPGYTFTENVARILNKKLLEHALKEERRQAPHGPPSLNSDSHMGETIKAEPGSIENQTVLLTAPWEL

Target ORF information:

RefSeq Version	XM_005623080.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris microtubule crosslinking factor 1 (MTCL1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623080.3

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCCGA GAACGACTAT CTCAAGGATG AATTGGATGA ACTCCGTGCT 
GAAATGGAAG AGATGAGAGA CAGTTACTTA GAGGAAGATG TTTACCAGCT GCAGGAGCTT 
CGGCGAGAGC TGGACCGTGC TCATAAAAAC TGCCGAATCC TGCAGTACCG CCTCAGGAAA 
GCTGAGCAGA AGAGCCTGAA AGTGGCTGAG ACTGGTCAGG TGGATGGTGA GCTCATCCGG 
AGTCTAGAGC AGGATTTAAA GGTAGCCAAA GATGTATCTG TCAGATTGCA TCATGAACTT 
GAAACCGTGG AGGAAAAGCG TGCTAAAGCA GAGGATGAAA ATGAAAGTCT CCGGCAGCAG 
ATGATTGAAG TGGAGATATC TAAACAGGCC CTCCAAAATG AGCTGGAGAG ACTGAAAGAG 
AGTTCCCTGA AAAGAAGAGG CAACCGAGAA ACATACAAAG AAAAGAAAAC CTTTAACCAG 
GATGACAGTG CCGATTTGAA GTGCCAGCTT CAGTTTGCCA AAGAGGAAGG CTCCCTGATG 
CGTAAAAAGA TGGCCAAGCT GGGGAAGGAG AAGGACGAGC TGGAACAGGA GCTCCAGAAG 
TACAAGGCCC TCTATGGAGA CGTGGACAGC CCCCTCCCCA CGGGGGAAGC CGGGGGGCCG 
CCCAGCACCA GGGAGGCTGA GCTGAAGCTG CGCTTGAAGT TGGTGGAGGA AGAGGCCAAC 
ATCTTGGGCC GGAAGATTGT GGAACTGGAG GTGGAGAACC GAGGCCTCAA AGCAGAAATG 
GAGGACATGA GGCTCCAGTA CGAGCGAGAG GGTACAGGCC GGGACCACAT CACAGGCATT 
CCTACCTCAC CCTTCGGTGA CTCCCTGGAG TCCTCGACGG AGCTCCGCCG GCACCTGCAG 
TTCGTGGAGG AGGAAGCGGA GCTGCTCCGG AGGTCCATAT CTGAGATCGA AGACCACAAC 
CGGCAGCTGA CCCATGAGCT GAACAAGTTC AAGTTTGAGC CTCGGCCGGA GCCAGGGTGG 
CTGGCGGATG GCGCGGGCAA GGGCGTGGGC AGCGGGGCCC CTCTGCAGGA GGAGCTCAAG 
TCGGCACGGC TACAGATCAA TGAGCTCAGT GGGAAGGTGC TGAAGCTGCA GTATGAGAAC 
CGGGTGCTAC TGTCCAATGT GCAGCGCTAT GACCTGGCTG CCCACCTAGG CCTGCGTGCC 
CCCAGCCCCC GGGACAGCGA CGCAGACAGC GACCAGGGCA AGAAGGAGAG CGATGGGGAG 
GAAGGCCGCC AGCCCCAGCC CAAGCGGGAG GGCCCTATTG GCGGGGAGAG CGACTCAGAG 
GAGATGTGCG AGAAGACGTC GGGCTTCGGC AGCGGAAAGC CATCGGAGGC GAGCGAGCCA 
TGTCCCGCCG AGCTCCTGCG GGCCAGGGAG GACACCGAGT GCCTGGCGAG CATAAAGCAC 
GAGGCTGAGC GCCTGGAGCG AACAGTGGAG CGCCTCATCA CGGACACCGC GGGCTTCGGG 
GACGATGTGG GGCTGCTGGG CAGTGGCGAG CCCTTGCCAG GGCCCGACAT CCAGGGGGCG 
GGGGGGACGG GCGAGGGGGG CAAGAAGGAG CCCCCACTGC TGGGGACCAT CAACTCGAGG 
ATGAAGGCTT TCAGGAAGGA GCTGCAGGCC TTCCTGGAGC AGGTGAACCG AATCGGGGAT 
GGCCTGCGGG AGCGCCTGGA AGGCCTGTCC CCCCTGCCTC ACCTCACAGA GTCTTCCAGC 
TTTCTCTCCA CCGTCACTTC CGTGTCCCGG GACTCCCCCG TCGGGAACCT GGGGAAGGAG 
CTGGTCCCGG ACCTGCAGTC CAAACTGAGA GGTCAGCTGG AGTGGCAGCC GGGGCAGGAG 
TGCGGGGACG AGCCAGAGCA GCTGCACCGC CGCGCTGATG GGGACGTCGG GAGCTGCCGG 
CAGGGAGGCC AGAGCTGCTT CAGTCTAGAG ATGGAGGAGG AGCACCTGTA CGCCTTGAGG 
TGGAAAGAAC TGGAAATGCA CAGCCTGGCT TTGCAGAACA CCCTCCATGA GCAAACCTGG 
AGTGATGAGA GGAACCTGAT GCAGCAGGAG CTCCGGTCTA TGAAGCAGAA CATTTTCCTT 
TTCTACGTCA AACTCAGATG GCTGCTGAGG CACTGGCGAC AAGGGAAGCA GATGGAGGAA 
GAAGGCGAGG ATTTCACTGA GAGCGAGCAT CCAGAGACCC TCCCCAGGCT TGGTGAGCTT 
GGAGTGCAGG GGGACCCGAA GGGGGACGGC CCAGACCAAG ATGACAACGG TCCGGGCTGC 
GGCTTTCCGC TGGGAGAGCA TCCTCCACAC TCCCCTGTGC AGATTGGTGA CCATGGATCG 
AGACTACAGC CTACACATGG GAGACAGCTC CACAGGCAGG TGGTGGAAAA CCAGCAGCTG 
TTCAGTGCAC TCAAGAGCCT GCTGGAGGAC CTGCGCGCCG AGCTGCGGGA GGACGAGCAT 
GCACGGCGGC GGCTGCAGCA GCAGTACGCC AGCGACAAAG CCGCCTGGGA CGTGGAGTGG 
GCTGCACTTA CCTGCCGCCT GGAACAGCTG GAAGAGAAGA CTGAGAAGAA TTTGGGAGAA 
GCATGCTCTT CCACCGACAG CAAAGGGGCA TTCAAGAAGG AAAGAGAGAT GCATCAGAAG 
CTCCTCACTG ACAGTCATGG CCTGGTCATG GACCTGCGCT GGCAGATCCA TCACAGTGAG 
AAGAACTGGA GCCGGGAGAA GGTGGAACTT CTTAGCCGTC TAGACAGAGA CCGGCAGGAA 
TGGGAGCAGC AGAAGAAGGA GCTCTTGTGG AGAATAGAAC AGTTGCAGAA AGAAAACAGT 
CCCCGGAGAA GTGGCAGTTT CCTCTGTGAT CAAAAAGAAG GCACCATCCG CCCCTTTGCC 
CATCCAGGAA GCCCCCGAAT GCCCCGTCCC TTGGGGATGT GGTCCTGTGC AGACACCGAC 
TCCACCCCAT TCGAAGACCG GCCGCTGTCC AAGCTGAAGG AATCAGACAG ATGTTCAGCA 
CGGGAAAACC TCTACTTGGA CGCCTTGTCT CTAGATGACG AGCCAGAGGA GCCTCCAGCC 
CGAGAGCCCC AGCGGGAGTT CAGGAACTGC CTCCCCCAGG AAGAAGAAAA TCACAAGGGA 
AATCTTCAAA GGGCCGTGTC TGTGTCCTCC ATGTCTGAGT TCCAGCGTCT GATGGACGTT 
TCTCCCTTCC TGCCGGAGAA GGGCGTCCCC GCCACCAGCA GCAAGGAGGA CATCACTCCG 
CCCCTGTCTC CTGATGATCT GAAGTACATC GAGGAGTTCA ACAGCAAGAA CTGGGACGAG 
AGGCCCCCGG AGCCCTGGGC AGACAGGACC GAGGGGGGGC GGGCAGGGAG CGAGGCTGGT 
GCAGAGCCTT TCCCTGACTC CTCCTGGTAC CTCACCACGA GTGTCACCAT GACTACGGAC 
ACCATGACCA GCCCAGAGCA CTGCCAGAAG CAGCCCCTTC GGAGCCCCAT GTGCGCGGAG 
CAGGCCGGGG TGCGGGTGCT GCACAGCCCG CCCGCGGTCC GTAGGATGGA TGGCATCGTG 
GTGGCGGGCA GTGAGGGCAA GGGCCGGGCC GAGCCCGAGT GCCCGTTTCC AACTAGCAGA 
GCCAGGGGGG GCACTGGAGA CACAAAAGGG GGTCCCTCAG AACACGTGCT CAGCAGGTGG 
CCTTGCGCCC CCTCCAGGCA CCCCCGAGAC TATGTGGAGG GAGGGCTGCG CCCCCTCGAA 
AGTGCCCTCT GCGCCCCCCT GGGTTTTGCC TCCTCGCTGC ACGGCCTGCA GATGTCCAAG 
AACATGAGTG ATGACATGAA GGAGGTGGCC TTCTCGGTCA GGAACGCCAT CTGCTCTGGC 
CCCCCCAAGC CTCCGCTCAA GGACATGGCC TGCCAGACCA ATGGGTCCCG GACGACGGGA 
ACACAGACCG TCCAGACCAT CACCATTGGC CTGCAGACCG AAGCCCTGCG TGGCAGCGCC 
GTCACCAGCA GCCCCCACAA GTGTCTCACA CCCAAGGCCG GGGGCGGGGC CACGCCCGCG 
TCATCTCCTT CCCGTGGCCT TCGGAGCCGG CAGATGGCCC CCGCCATCGA GAAGGTGCAG 
GCCAAGTTCG AACGCACGTG CTGCTCCCCC AAGTACGGGT CTCCCAAGCT GCAGAGGAAG 
CCCCTCCCCA AAGCTGAGCC GCCCAACAGC AGGACCTCCC TGGGGCTGGC CCCAAAGGGG 
TGCAGCGAGT CCGCCTGGGC CCGTTCCACC ACCACGAGGG AGAGCCCGGT GCACACCGCC 
ATCAACGATG GCCTCTCCAG CCTCTTCAAC ATCATCGACC ACAGCCCCGT GGTGCAGGAC 
CCCTTCCAGA AGGGGACTCG GGCCGGGAGC CGATCTCGCT CAGCAGAACC CCGGCCCGAG 
CTGGGCCCAG GCCAGGAAAC GGGCCCCAGT TCCCGGGGCC GGTCCCCCAG CCCCATCGGG 
GCTGGCTCAG AGACTTGGAG GGAGGAAGGG GGAGAGGGCA CGCCCTTGAG GCAGGACTTA 
TCCGCTCCCC CTGGTTACAC GTTCACTGAG AACGTAGCCC GGATCCTCAA CAAGAAGCTG 
TTGGAGCATG CCTTAAAGGA GGAGAGGAGG CAGGCCCCCC ACGGGCCCCC AAGTCTTAAC 
AGTGACAGCC ACATGGGAGA AACCATCAAA GCTGAACCAG GGTCCATCGA GAACCAAACT 
GTCTTACTAA CTGCCCCCTG GGAACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MTCL1
Entrez Gene ID	490545
Full Name	microtubule crosslinking factor 1
Synonyms	CCDC165,SOGA2
General protein information	Preferred Names microtubule crosslinking factor 1 Names microtubule cross-linking factor 1 SOGA family member 2 coiled-coil domain containing 165
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	MTCL1	NP_056025.2
Pan troglodytes (chimpanzee)	SOGA2	XP_001140422.2
Canis lupus familiaris (dog)	SOGA2	XP_005623132.1
Bos taurus (cattle)	CCDC165	XP_005224355.1
Danio rerio (zebrafish)	LOC101884972	XP_005171514.1
	LOC706392	XP_001100204.2
Mus musculus (house mouse)	Soga2	NP_766551.3
Rattus norvegicus (Norway rat)	Soga2	XP_006245692.1
Gallus gallus (chicken)	SOGA2	XP_004939798.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MTCL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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