VIL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	VIL1
Entrez Gene ID	488522
Full Name	villin 1
General protein information	Preferred Names villin 1 Names villin-1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_545642.4	XP_545642.2	villin-1
XM_545642.5	XP_545642.2	villin-1

Pathways from BioSystems

Homologies

	VIL1	XP_001090524.1
Bos taurus (cattle)	VIL1	NP_001013609.1
Mus musculus (house mouse)	Vil1	NP_033535.2
Arabidopsis thaliana (thale cress)	AT5G57320	NP_200542.1
Arabidopsis thaliana (thale cress)	VLN4	NP_194745.1
Xenopus tropicalis (tropical clawed frog)	vil1	NP_001096367.1
Gallus gallus (chicken)	VIL1	NP_990773.1
Homo sapiens (human)	VIL1	NP_009058.2
Pan troglodytes (chimpanzee)	VIL1	XP_001157250.2
Danio rerio (zebrafish)	vil1	NP_956532.1
Oryza sativa (rice)	Os08g0240800	NP_001061335.1
Oryza sativa (rice)	Os06g0659300	NP_001058263.1
Canis lupus familiaris (dog)	VIL1	XP_545642.2
Rattus norvegicus (Norway rat)	Vil1	NP_001101694.1
Oryza sativa (rice)	Os04g0604000	NP_001053784.1

Gene Ontology

The following VIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe31493	XM_545642.5 Latest version!	Canis lupus familiaris villin 1 (VIL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OCe31493	XM_545642.4	Canis lupus familiaris villin 1 (VIL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VIL1 ( XM_545642.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_545642.2 Canis lupus familiaris villin 1 (VIL1), mRNA.

Price & Availability↑

CloneID	OCe31493
Clone ID Related Accession (Same CDS sequence)	XM_545642.4 , XM_545642.5
Accession Version	XM_545642.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	villin-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_545642.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGAC TGACCACCCA AGTCAAAAAC TCACTCAACA TCACCACCCC CGGGGTGCAG 
ATATGGAGGA TCGAGGCCAT GAAGATGGTT CCTGTTCCTT CCAGCACCTT CGGTAGCTTC 
TTTGACGGTG ACTGCTACAT AGTCTTGGCT ATCCACAAGA CGGGCAGCAA TCTGTCCTAT 
GACATCCACT ACTGGATCGG CCAGGCCTCG TCCCAGGACG AGCAAGGGGC GGCTGCCATC 
TACACCACAC AGATGGATGA CTTCCTGAAG GGCCGGGCTG TCCAGCACCG GGAGGTCCAG 
GGCAACGAGA GCGAGGCCTT CCGGGGCTAC TTCAAGCAGG GCATTGTGAT CCAGAAGGGG 
GGTGTGGCTT CCGGCATGAA GCAAGTGGAG ACCAACTCCT ACGAGATCCA GCGGCTGCTG 
CATGTCAAGG GCAAGAGGAA CGTGGTGGCC GGAGAGGTGG AGCTGGCTTG GAAGAGTTTC 
AACCGCGGGG ATGTTTTCCT CCTGGACCTG GGGAAGCTCA TCATCCAGTG GAACGGGCCC 
GAGAGCAACC GCATGGAGAG ACTCAGGGGC ATGACCCTGG CCAAGGAGAT CCGAGACCAG 
GAGCGCGGCG GGCGGACCTA CGTGGCGGTG GTGGAGGGGG ACAACGAGAA GGCCACCCCG 
CAGCTGATGG AGATCATGAC CCACGTGCTG GGCCCGCGCG GCACCCTGAG GGCGGCGGTG 
CCGGACAACG AGGTGGAGCC GGCGGTCAAG GCCGCCCTCA AGCTGTACCA CGTGTCGGAC 
GCGGAGGGAA AGCTGGTGGT GCGAGAAGTC GCCACGAGGC CCCTCACCCA AGACCTGCTC 
AGCCACGAGG ACTGTTATAT CCTGGACCAG GGGGGCCTGA AGATCTACGT GTGGAAGGGG 
AAGAGGGCCA ACGCCCAGGA GAAGACGGGA GCCATGAACC AGGCACTGAA CTTTATCAAA 
GCAAAGCAGT ACCCGCCCAG CACACAGGTG GAGGTGCAGA ATGATGGGGC CGAGTCAGCT 
GTGTTCCAGC AGCTCTTCCA GAAGTGGACC CTGCCCAATC GGACTTCAGG CCTGGGCAAA 
ACCCATGCCG TGGGTTCCGT GGCCAAGGTG GAGCAAGTGA AGTTTGATGC CACATCCATG 
CATGTCCAGC CTCAGGTGGC TGCCCAGAAG AAGATGGTGG ATGACGGGAG CGGGGAGGTG 
CAGGTGTGGC GCATTGAGAA CCTAGAGCTG GTGCCGGTGG AGTCCAAGTG GCTAGGCCAC 
TTCTACGGGG GTGACTGCTA CCTGCTGCTC TACACCTACC TCATTGGCGA GAAGAAGAAC 
TACTTGCTCT ACATTTGGCA GGGTAGCCAA GCCAGCCAGG ATGAAATAAC AGCCTCTGCC 
TATCAAGCTG TCACCCTAGA CCAGAAGTAC AACAACGAAC CGGTCCAGAT CCGGGTCCCG 
ATGGGCAAGG AGCCACCACA CCTCATGGCC ATCTTCAAGG GACAAATGGT GGTTTACCAG 
GGAGGCACCT CCCGGGCCAA CACACAGGAG CCAGTGCCCT CCACACGGCT GTTCCAGGTC 
CAGGGAACCA GCGCCAACAA CACCAAGGCC TTTGAGGTCC AGCCCCGGGC CAGCTCCCTC 
AACTCCAATG ACGTCTTCAT CCTTAAGACC CAGTCCTGCT GCTATCTGTG GTGCGGGAAG 
GGCTGCAGTG GGGACGAGCG GGAGATGGCC AAGATGGTTG CTGACACCAT CTCCCGGACA 
GAGAAGCAAG TGGTGGTGGA AGGGCAGGAG CCGGCCAACT TCTGGGTAGC CCTGGGTGGA 
AAGGCTCCCT ATGCCAACAC CAAGAGATTG CAGGAGGAAA CCCTGGCCAT CACGCCCCGG 
CTCTTTGAAT GTTCCAACCA GACCGGGCGC TTTCTGGCCA CAGAAATCCC TGACTTCAAT 
CAAGATGACC TGGAAGAGGA CGATGTGTTC CTGCTAGATG TCTGGGACCA GGTCTTCTTC 
TGGATCGGGA AGCACGCCAA CGAGGAGGAG AAGAGAGCCG CAGCCGTCAC GGCACAGGAG 
TATCTCAAGA CTCACCCCAG CGGCCGTGAC CCCGACACCC CCATCATCGT GGTGAAGCAG 
GGATACGAGC CCTCCACCTT CACCGGCTGG TTCCTGGCGT GGGATCCCTT CAAGTGGAGT 
AACACCAAAT CCTATGAGGA CCTGAAGGCG GAGCTCCAAA GCCCTGAGGA CTGGAGCCAG 
ATCACTGCTG AGATCACCAA ACCTAAGCCG AACACGTTCA CCGCTAACAC CAGCCTCATT 
TCTGGGCCTC TACCCATCTT TCCCCTGGAG CAGCTGGTGA ACAAGTCTGT GGAGGAGCTC 
CCTGAGGGTG TAGACCCCAC TAGGAAGGAG GAGCACCTGT CTGTCGAGGA TTTCACCAAG 
GTCTTGGGAA TGACACCCAC GGCCTTCTCT GCCCTCCCTA AATGGAAGCA ACAAAACCTC 
AAGAAAGAAA AAGGCCTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_545642.2
CDS	98..2581
Translation	MTRLTTQVKNSLNITTPGVQIWRIEAMKMVPVPSSTFGSFFDGDCYIVLAIHKTGSNLSYDIHYWIGQASSQDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGIVIQKGGVASGMKQVETNSYEIQRLLHVKGKRNVVAGEVELAWKSFNRGDVFLLDLGKLIIQWNGPESNRMERLRGMTLAKEIRDQERGGRTYVAVVEGDNEKATPQLMEIMTHVLGPRGTLRAAVPDNEVEPAVKAALKLYHVSDAEGKLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKRANAQEKTGAMNQALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWTLPNRTSGLGKTHAVGSVAKVEQVKFDATSMHVQPQVAAQKKMVDDGSGEVQVWRIENLELVPVESKWLGHFYGGDCYLLLYTYLIGEKKNYLLYIWQGSQASQDEITASAYQAVTLDQKYNNEPVQIRVPMGKEPPHLMAIFKGQMVVYQGGTSRANTQEPVPSTRLFQVQGTSANNTKAFEVQPRASSLNSNDVFILKTQSCCYLWCGKGCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWVALGGKAPYANTKRLQEETLAITPRLFECSNQTGRFLATEIPDFNQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKRAAAVTAQEYLKTHPSGRDPDTPIIVVKQGYEPSTFTGWFLAWDPFKWSNTKSYEDLKAELQSPEDWSQITAEITKPKPNTFTANTSLISGPLPIFPLEQLVNKSVEELPEGVDPTRKEEHLSVEDFTKVLGMTPTAFSALPKWKQQNLKKEKGLF

Target ORF information:

RefSeq Version	XM_545642.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris villin 1 (VIL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_545642.4

ORF Insert Sequence:

ATGACCAGAC TGACCACCCA AGTCAAAAAC TCACTCAACA TCACCACCCC CGGGGTGCAG 
ATATGGAGGA TCGAGGCCAT GAAGATGGTT CCTGTTCCTT CCAGCACCTT CGGTAGCTTC 
TTTGACGGTG ACTGCTACAT AGTCTTGGCT ATCCACAAGA CGGGCAGCAA TCTGTCCTAT 
GACATCCACT ACTGGATCGG CCAGGCCTCG TCCCAGGACG AGCAAGGGGC GGCTGCCATC 
TACACCACAC AGATGGATGA CTTCCTGAAG GGCCGGGCTG TCCAGCACCG GGAGGTCCAG 
GGCAACGAGA GCGAGGCCTT CCGGGGCTAC TTCAAGCAGG GCATTGTGAT CCAGAAGGGG 
GGTGTGGCTT CCGGCATGAA GCAAGTGGAG ACCAACTCCT ACGAGATCCA GCGGCTGCTG 
CATGTCAAGG GCAAGAGGAA CGTGGTGGCC GGAGAGGTGG AGCTGGCTTG GAAGAGTTTC 
AACCGCGGGG ATGTTTTCCT CCTGGACCTG GGGAAGCTCA TCATCCAGTG GAACGGGCCC 
GAGAGCAACC GCATGGAGAG ACTCAGGGGC ATGACCCTGG CCAAGGAGAT CCGAGACCAG 
GAGCGCGGCG GGCGGACCTA CGTGGCGGTG GTGGAGGGGG ACAACGAGAA GGCCACCCCG 
CAGCTGATGG AGATCATGAC CCACGTGCTG GGCCCGCGCG GCACCCTGAG GGCGGCGGTG 
CCGGACAACG AGGTGGAGCC GGCGGTCAAG GCCGCCCTCA AGCTGTACCA CGTGTCGGAC 
GCGGAGGGAA AGCTGGTGGT GCGAGAAGTC GCCACGAGGC CCCTCACCCA AGACCTGCTC 
AGCCACGAGG ACTGTTATAT CCTGGACCAG GGGGGCCTGA AGATCTACGT GTGGAAGGGG 
AAGAGGGCCA ACGCCCAGGA GAAGACGGGA GCCATGAACC AGGCACTGAA CTTTATCAAA 
GCAAAGCAGT ACCCGCCCAG CACACAGGTG GAGGTGCAGA ATGATGGGGC CGAGTCAGCT 
GTGTTCCAGC AGCTCTTCCA GAAGTGGACC CTGCCCAATC GGACTTCAGG CCTGGGCAAA 
ACCCATGCCG TGGGTTCCGT GGCCAAGGTG GAGCAAGTGA AGTTTGATGC CACATCCATG 
CATGTCCAGC CTCAGGTGGC TGCCCAGAAG AAGATGGTGG ATGACGGGAG CGGGGAGGTG 
CAGGTGTGGC GCATTGAGAA CCTAGAGCTG GTGCCGGTGG AGTCCAAGTG GCTAGGCCAC 
TTCTACGGGG GTGACTGCTA CCTGCTGCTC TACACCTACC TCATTGGCGA GAAGAAGAAC 
TACTTGCTCT ACATTTGGCA GGGTAGCCAA GCCAGCCAGG ATGAAATAAC AGCCTCTGCC 
TATCAAGCTG TCACCCTAGA CCAGAAGTAC AACAACGAAC CGGTCCAGAT CCGGGTCCCG 
ATGGGCAAGG AGCCACCACA CCTCATGGCC ATCTTCAAGG GACAAATGGT GGTTTACCAG 
GGAGGCACCT CCCGGGCCAA CACACAGGAG CCAGTGCCCT CCACACGGCT GTTCCAGGTC 
CAGGGAACCA GCGCCAACAA CACCAAGGCC TTTGAGGTCC AGCCCCGGGC CAGCTCCCTC 
AACTCCAATG ACGTCTTCAT CCTTAAGACC CAGTCCTGCT GCTATCTGTG GTGCGGGAAG 
GGCTGCAGTG GGGACGAGCG GGAGATGGCC AAGATGGTTG CTGACACCAT CTCCCGGACA 
GAGAAGCAAG TGGTGGTGGA AGGGCAGGAG CCGGCCAACT TCTGGGTAGC CCTGGGTGGA 
AAGGCTCCCT ATGCCAACAC CAAGAGATTG CAGGAGGAAA CCCTGGCCAT CACGCCCCGG 
CTCTTTGAAT GTTCCAACCA GACCGGGCGC TTTCTGGCCA CAGAAATCCC TGACTTCAAT 
CAAGATGACC TGGAAGAGGA CGATGTGTTC CTGCTAGATG TCTGGGACCA GGTCTTCTTC 
TGGATCGGGA AGCACGCCAA CGAGGAGGAG AAGAGAGCCG CAGCCGTCAC GGCACAGGAG 
TATCTCAAGA CTCACCCCAG CGGCCGTGAC CCCGACACCC CCATCATCGT GGTGAAGCAG 
GGATACGAGC CCTCCACCTT CACCGGCTGG TTCCTGGCGT GGGATCCCTT CAAGTGGAGT 
AACACCAAAT CCTATGAGGA CCTGAAGGCG GAGCTCCAAA GCCCTGAGGA CTGGAGCCAG 
ATCACTGCTG AGATCACCAA ACCTAAGCCG AACACGTTCA CCGCTAACAC CAGCCTCATT 
TCTGGGCCTC TACCCATCTT TCCCCTGGAG CAGCTGGTGA ACAAGTCTGT GGAGGAGCTC 
CCTGAGGGTG TAGACCCCAC TAGGAAGGAG GAGCACCTGT CTGTCGAGGA TTTCACCAAG 
GTCTTGGGAA TGACACCCAC GGCCTTCTCT GCCCTCCCTA AATGGAAGCA ACAAAACCTC 
AAGAAAGAAA AAGGCCTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VIL1 ( XM_545642.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_545642.2 Canis lupus familiaris villin 1 (VIL1), mRNA.

Price & Availability↑

CloneID	OCe31493
Clone ID Related Accession (Same CDS sequence)	XM_545642.4 , XM_545642.5
Accession Version	XM_545642.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	villin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_545642.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGAC TGACCACCCA AGTCAAAAAC TCACTCAACA TCACCACCCC CGGGGTGCAG 
ATATGGAGGA TCGAGGCCAT GAAGATGGTT CCTGTTCCTT CCAGCACCTT CGGTAGCTTC 
TTTGACGGTG ACTGCTACAT AGTCTTGGCT ATCCACAAGA CGGGCAGCAA TCTGTCCTAT 
GACATCCACT ACTGGATCGG CCAGGCCTCG TCCCAGGACG AGCAAGGGGC GGCTGCCATC 
TACACCACAC AGATGGATGA CTTCCTGAAG GGCCGGGCTG TCCAGCACCG GGAGGTCCAG 
GGCAACGAGA GCGAGGCCTT CCGGGGCTAC TTCAAGCAGG GCATTGTGAT CCAGAAGGGG 
GGTGTGGCTT CCGGCATGAA GCAAGTGGAG ACCAACTCCT ACGAGATCCA GCGGCTGCTG 
CATGTCAAGG GCAAGAGGAA CGTGGTGGCC GGAGAGGTGG AGCTGGCTTG GAAGAGTTTC 
AACCGCGGGG ATGTTTTCCT CCTGGACCTG GGGAAGCTCA TCATCCAGTG GAACGGGCCC 
GAGAGCAACC GCATGGAGAG ACTCAGGGGC ATGACCCTGG CCAAGGAGAT CCGAGACCAG 
GAGCGCGGCG GGCGGACCTA CGTGGCGGTG GTGGAGGGGG ACAACGAGAA GGCCACCCCG 
CAGCTGATGG AGATCATGAC CCACGTGCTG GGCCCGCGCG GCACCCTGAG GGCGGCGGTG 
CCGGACAACG AGGTGGAGCC GGCGGTCAAG GCCGCCCTCA AGCTGTACCA CGTGTCGGAC 
GCGGAGGGAA AGCTGGTGGT GCGAGAAGTC GCCACGAGGC CCCTCACCCA AGACCTGCTC 
AGCCACGAGG ACTGTTATAT CCTGGACCAG GGGGGCCTGA AGATCTACGT GTGGAAGGGG 
AAGAGGGCCA ACGCCCAGGA GAAGACGGGA GCCATGAACC AGGCACTGAA CTTTATCAAA 
GCAAAGCAGT ACCCGCCCAG CACACAGGTG GAGGTGCAGA ATGATGGGGC CGAGTCAGCT 
GTGTTCCAGC AGCTCTTCCA GAAGTGGACC CTGCCCAATC GGACTTCAGG CCTGGGCAAA 
ACCCATGCCG TGGGTTCCGT GGCCAAGGTG GAGCAAGTGA AGTTTGATGC CACATCCATG 
CATGTCCAGC CTCAGGTGGC TGCCCAGAAG AAGATGGTGG ATGACGGGAG CGGGGAGGTG 
CAGGTGTGGC GCATTGAGAA CCTAGAGCTG GTGCCGGTGG AGTCCAAGTG GCTAGGCCAC 
TTCTACGGGG GTGACTGCTA CCTGCTGCTC TACACCTACC TCATTGGCGA GAAGAAGAAC 
TACTTGCTCT ACATTTGGCA GGGTAGCCAA GCCAGCCAGG ATGAAATAAC AGCCTCTGCC 
TATCAAGCTG TCACCCTAGA CCAGAAGTAC AACAACGAAC CGGTCCAGAT CCGGGTCCCG 
ATGGGCAAGG AGCCACCACA CCTCATGGCC ATCTTCAAGG GACAAATGGT GGTTTACCAG 
GGAGGCACCT CCCGGGCCAA CACACAGGAG CCAGTGCCCT CCACACGGCT GTTCCAGGTC 
CAGGGAACCA GCGCCAACAA CACCAAGGCC TTTGAGGTCC AGCCCCGGGC CAGCTCCCTC 
AACTCCAATG ACGTCTTCAT CCTTAAGACC CAGTCCTGCT GCTATCTGTG GTGCGGGAAG 
GGCTGCAGTG GGGACGAGCG GGAGATGGCC AAGATGGTTG CTGACACCAT CTCCCGGACA 
GAGAAGCAAG TGGTGGTGGA AGGGCAGGAG CCGGCCAACT TCTGGGTAGC CCTGGGTGGA 
AAGGCTCCCT ATGCCAACAC CAAGAGATTG CAGGAGGAAA CCCTGGCCAT CACGCCCCGG 
CTCTTTGAAT GTTCCAACCA GACCGGGCGC TTTCTGGCCA CAGAAATCCC TGACTTCAAT 
CAAGATGACC TGGAAGAGGA CGATGTGTTC CTGCTAGATG TCTGGGACCA GGTCTTCTTC 
TGGATCGGGA AGCACGCCAA CGAGGAGGAG AAGAGAGCCG CAGCCGTCAC GGCACAGGAG 
TATCTCAAGA CTCACCCCAG CGGCCGTGAC CCCGACACCC CCATCATCGT GGTGAAGCAG 
GGATACGAGC CCTCCACCTT CACCGGCTGG TTCCTGGCGT GGGATCCCTT CAAGTGGAGT 
AACACCAAAT CCTATGAGGA CCTGAAGGCG GAGCTCCAAA GCCCTGAGGA CTGGAGCCAG 
ATCACTGCTG AGATCACCAA ACCTAAGCCG AACACGTTCA CCGCTAACAC CAGCCTCATT 
TCTGGGCCTC TACCCATCTT TCCCCTGGAG CAGCTGGTGA ACAAGTCTGT GGAGGAGCTC 
CCTGAGGGTG TAGACCCCAC TAGGAAGGAG GAGCACCTGT CTGTCGAGGA TTTCACCAAG 
GTCTTGGGAA TGACACCCAC GGCCTTCTCT GCCCTCCCTA AATGGAAGCA ACAAAACCTC 
AAGAAAGAAA AAGGCCTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_545642.2
CDS	120..2603
Translation	MTRLTTQVKNSLNITTPGVQIWRIEAMKMVPVPSSTFGSFFDGDCYIVLAIHKTGSNLSYDIHYWIGQASSQDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGIVIQKGGVASGMKQVETNSYEIQRLLHVKGKRNVVAGEVELAWKSFNRGDVFLLDLGKLIIQWNGPESNRMERLRGMTLAKEIRDQERGGRTYVAVVEGDNEKATPQLMEIMTHVLGPRGTLRAAVPDNEVEPAVKAALKLYHVSDAEGKLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKRANAQEKTGAMNQALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWTLPNRTSGLGKTHAVGSVAKVEQVKFDATSMHVQPQVAAQKKMVDDGSGEVQVWRIENLELVPVESKWLGHFYGGDCYLLLYTYLIGEKKNYLLYIWQGSQASQDEITASAYQAVTLDQKYNNEPVQIRVPMGKEPPHLMAIFKGQMVVYQGGTSRANTQEPVPSTRLFQVQGTSANNTKAFEVQPRASSLNSNDVFILKTQSCCYLWCGKGCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWVALGGKAPYANTKRLQEETLAITPRLFECSNQTGRFLATEIPDFNQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKRAAAVTAQEYLKTHPSGRDPDTPIIVVKQGYEPSTFTGWFLAWDPFKWSNTKSYEDLKAELQSPEDWSQITAEITKPKPNTFTANTSLISGPLPIFPLEQLVNKSVEELPEGVDPTRKEEHLSVEDFTKVLGMTPTAFSALPKWKQQNLKKEKGLF

Target ORF information:

RefSeq Version	XM_545642.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris villin 1 (VIL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_545642.5

ORF Insert Sequence:

ATGACCAGAC TGACCACCCA AGTCAAAAAC TCACTCAACA TCACCACCCC CGGGGTGCAG 
ATATGGAGGA TCGAGGCCAT GAAGATGGTT CCTGTTCCTT CCAGCACCTT CGGTAGCTTC 
TTTGACGGTG ACTGCTACAT AGTCTTGGCT ATCCACAAGA CGGGCAGCAA TCTGTCCTAT 
GACATCCACT ACTGGATCGG CCAGGCCTCG TCCCAGGACG AGCAAGGGGC GGCTGCCATC 
TACACCACAC AGATGGATGA CTTCCTGAAG GGCCGGGCTG TCCAGCACCG GGAGGTCCAG 
GGCAACGAGA GCGAGGCCTT CCGGGGCTAC TTCAAGCAGG GCATTGTGAT CCAGAAGGGG 
GGTGTGGCTT CCGGCATGAA GCAAGTGGAG ACCAACTCCT ACGAGATCCA GCGGCTGCTG 
CATGTCAAGG GCAAGAGGAA CGTGGTGGCC GGAGAGGTGG AGCTGGCTTG GAAGAGTTTC 
AACCGCGGGG ATGTTTTCCT CCTGGACCTG GGGAAGCTCA TCATCCAGTG GAACGGGCCC 
GAGAGCAACC GCATGGAGAG ACTCAGGGGC ATGACCCTGG CCAAGGAGAT CCGAGACCAG 
GAGCGCGGCG GGCGGACCTA CGTGGCGGTG GTGGAGGGGG ACAACGAGAA GGCCACCCCG 
CAGCTGATGG AGATCATGAC CCACGTGCTG GGCCCGCGCG GCACCCTGAG GGCGGCGGTG 
CCGGACAACG AGGTGGAGCC GGCGGTCAAG GCCGCCCTCA AGCTGTACCA CGTGTCGGAC 
GCGGAGGGAA AGCTGGTGGT GCGAGAAGTC GCCACGAGGC CCCTCACCCA AGACCTGCTC 
AGCCACGAGG ACTGTTATAT CCTGGACCAG GGGGGCCTGA AGATCTACGT GTGGAAGGGG 
AAGAGGGCCA ACGCCCAGGA GAAGACGGGA GCCATGAACC AGGCACTGAA CTTTATCAAA 
GCAAAGCAGT ACCCGCCCAG CACACAGGTG GAGGTGCAGA ATGATGGGGC CGAGTCAGCT 
GTGTTCCAGC AGCTCTTCCA GAAGTGGACC CTGCCCAATC GGACTTCAGG CCTGGGCAAA 
ACCCATGCCG TGGGTTCCGT GGCCAAGGTG GAGCAAGTGA AGTTTGATGC CACATCCATG 
CATGTCCAGC CTCAGGTGGC TGCCCAGAAG AAGATGGTGG ATGACGGGAG CGGGGAGGTG 
CAGGTGTGGC GCATTGAGAA CCTAGAGCTG GTGCCGGTGG AGTCCAAGTG GCTAGGCCAC 
TTCTACGGGG GTGACTGCTA CCTGCTGCTC TACACCTACC TCATTGGCGA GAAGAAGAAC 
TACTTGCTCT ACATTTGGCA GGGTAGCCAA GCCAGCCAGG ATGAAATAAC AGCCTCTGCC 
TATCAAGCTG TCACCCTAGA CCAGAAGTAC AACAACGAAC CGGTCCAGAT CCGGGTCCCG 
ATGGGCAAGG AGCCACCACA CCTCATGGCC ATCTTCAAGG GACAAATGGT GGTTTACCAG 
GGAGGCACCT CCCGGGCCAA CACACAGGAG CCAGTGCCCT CCACACGGCT GTTCCAGGTC 
CAGGGAACCA GCGCCAACAA CACCAAGGCC TTTGAGGTCC AGCCCCGGGC CAGCTCCCTC 
AACTCCAATG ACGTCTTCAT CCTTAAGACC CAGTCCTGCT GCTATCTGTG GTGCGGGAAG 
GGCTGCAGTG GGGACGAGCG GGAGATGGCC AAGATGGTTG CTGACACCAT CTCCCGGACA 
GAGAAGCAAG TGGTGGTGGA AGGGCAGGAG CCGGCCAACT TCTGGGTAGC CCTGGGTGGA 
AAGGCTCCCT ATGCCAACAC CAAGAGATTG CAGGAGGAAA CCCTGGCCAT CACGCCCCGG 
CTCTTTGAAT GTTCCAACCA GACCGGGCGC TTTCTGGCCA CAGAAATCCC TGACTTCAAT 
CAAGATGACC TGGAAGAGGA CGATGTGTTC CTGCTAGATG TCTGGGACCA GGTCTTCTTC 
TGGATCGGGA AGCACGCCAA CGAGGAGGAG AAGAGAGCCG CAGCCGTCAC GGCACAGGAG 
TATCTCAAGA CTCACCCCAG CGGCCGTGAC CCCGACACCC CCATCATCGT GGTGAAGCAG 
GGATACGAGC CCTCCACCTT CACCGGCTGG TTCCTGGCGT GGGATCCCTT CAAGTGGAGT 
AACACCAAAT CCTATGAGGA CCTGAAGGCG GAGCTCCAAA GCCCTGAGGA CTGGAGCCAG 
ATCACTGCTG AGATCACCAA ACCTAAGCCG AACACGTTCA CCGCTAACAC CAGCCTCATT 
TCTGGGCCTC TACCCATCTT TCCCCTGGAG CAGCTGGTGA ACAAGTCTGT GGAGGAGCTC 
CCTGAGGGTG TAGACCCCAC TAGGAAGGAG GAGCACCTGT CTGTCGAGGA TTTCACCAAG 
GTCTTGGGAA TGACACCCAC GGCCTTCTCT GCCCTCCCTA AATGGAAGCA ACAAAACCTC 
AAGAAAGAAA AAGGCCTGTT TTGA