NGEF cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NGEF
Entrez Gene ID	486165
Full Name	neuronal guanine nucleotide exchange factor
General protein information	Preferred Names neuronal guanine nucleotide exchange factor Names ephexin-1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014107552.1	XP_013963027.1	ephexin-1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NGEF	XP_005635921.1
Danio rerio (zebrafish)	LOC569090	XP_697546.3
Xenopus tropicalis (tropical clawed frog)	ngef	XP_004917852.1
Bos taurus (cattle)	NGEF	NP_001070394.1
Rattus norvegicus (Norway rat)	Ngef	NP_001129713.1
Pan troglodytes (chimpanzee)	NGEF	XP_516157.3
Homo sapiens (human)	NGEF	NP_062824.2
	LOC713917	XP_001114604.1
Mus musculus (house mouse)	Ngef	NP_001104784.1
Gallus gallus (chicken)	NGEF	NP_001010841.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007452	XP_308411.4

Gene Ontology

The following NGEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe25835	XM_014107552.1 Latest version!	Canis lupus familiaris neuronal guanine nucleotide exchange factor (NGEF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NGEF ( XM_014107552.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963027.1 Canis lupus familiaris neuronal guanine nucleotide exchange factor (NGEF), mRNA.

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CloneID	OCe25835
Clone ID Related Accession (Same CDS sequence)	XM_014107552.1
Accession Version	XM_014107552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ephexin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGGT GGAGAATCCC AAAACAGCCA GACCCAGGAG CCAAGAGAAC AAGCGAGTCG 
TCCTCCACCC CGGGAAATGG GGCCACGCCC GAGGAGTGCC CGGCGCTGGC CGACAGCCCC 
ACCACGCTCA CCGAGGCCCT GCAGATGATC CACCCCATCC CCGCCGACTC CTGGAGAAAC 
CTCATCGAAC AAATAGGGCT CCTGTATCAG GAATACCGAG ATAAATCCAC GCTCCAAGAA 
ATTGAAACCA GGAGGCAGCA GGATGCAGAG ATACACACCA ATGCCCATGG CTCCCCGGCC 
AGCGAGGAGA CCCCAGAAGA GGAGGAGGAC GAGGAGGAGG AGGAGGACGA GCCGGCAAGC 
CCGCCCGAGA GGAAGGCTCT GCCCCAGATC TGCCTCCTCA GTAACCCCCA CTCGAGATTT 
AACCTCTGGC AGGACCTGCC CGAGATCCAG AGCAGCGGGG TGCTGGAAAT CCTCCAGCCC 
GAGGAGATCA AGCTGCAGGA GGCCATGTTC GAGCTGGTCA CTTCTGAGGC CTCCTACTAC 
AAAAGCCTGA GCCTGCTCGT GTCTCACTTC ATGGAGAATG AACGTCTGAA GAAGATCCTG 
CACCCGTCTG AGGCACACAT CCTCTTCTCC AATGTCCTGG ACGTCATGGC CGTCAGTGAA 
CGGTTTCTCC TGGAGCTGGA GCGCCGGATG GAGGAGAACA TCGTCATTTC GGACGTGTGC 
GACATCGTGT ACCATTACGC GGCCAACCAC TTCTCCGTGT ACATCACCTA CGTCAGCAAC 
CAGACCTACC AGGAGAGGAC CTACAAGCAG TTGCTCCAGG AGAAGGCGGC CTTTCGGGAG 
CTGATTGCGC AGTTGGAGCT GGACCCCAAG TGCAGGGGGC TGCCTCTGTC CTCCTTCCTC 
ATCCTGCCTT TCCAGAGGAT CACGCGCCTC AAGCTGTTGA TCCAGAACAT TCTGAAGAGA 
GTGGAAGAAA GGTCTGAGCG CGAGTGCACT GCTTTGGAGG CCCACAAGGA ACTGGAGATG 
GTGGTAAAAG CGTGCAACGA GGGTGTCAGG AAAATGAGCC GCACAGAGCA GATGATCAGC 
ATTCAGAAGA AGATGGAGTT TAAGATCAAG TCCGTGCCCA TCATCTCCCA CTCCCGCTGG 
CTGCTGAAGC AGGGTGAGCT GCAGCAGATG TCAGGCCCCA AGACCTCCCG AACCCTGCGG 
ACCAAGAAAC TCTTCCGGGA AATTTACCTC TTCCTCTTCA ACGACCTGCT GGTGATCTGC 
CGGCAGATCC CCGGAGACAA GTACCAAGTG TTTGACTCGG CCCCGAGGGG CCTCCTCCGG 
GTGGAGGAGC TGGAGGACCA GGGCCAGACG CTGGCCAACG TGTTCATCCT GCGGCTGCTG 
GAGAACGCGG ACGACCGGGA GGCCACCTAC ATGCTGAAGG CGTCTTCTCA GAGTGAGATG 
AAGCGTTGGA TGACCTCACT GGCCCCCAAC CGGAGAACCA AGTTTGTTTC CTTCACTTCC 
CGGCTGCTGG ACTGTCCCCA GGTCCAGTGT GTGCACCCGT ATGTGGCCCA GCAGCCAGAC 
GAGCTGACAC TGGAGCTGGC TGACATTCTG AACATTCTGG AAAAGACCGA AGACGGGTGG 
ATCTTTGGCG AGCGTCTGCA TGACCAGGAG AGAGGCTGGT TCCCCAGCTC CATGACTGAG 
GAGATCTTGA ATCCCAAGAT CCGGTCCCAG AACCTCAAGG AATGTTTCCG AGTCCACAAG 
ATGGACGACC CTCAGCGCAG CCAGAACAAG GATCGCAGGA AGCTGGGCAG CCGAAATCGG 
CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963027.1
CDS	145..1950
Translation	MLRWRIPKQPDPGAKRTSESSSTPGNGATPEECPALADSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIHTNAHGSPASEETPEEEEDEEEEEDEPASPPERKALPQICLLSNPHSRFNLWQDLPEIQSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLSLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELERRMEENIVISDVCDIVYHYAANHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPLSSFLILPFQRITRLKLLIQNILKRVEERSERECTALEAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILEKTEDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	XM_014107552.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris neuronal guanine nucleotide exchange factor (NGEF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107552.1

ORF Insert Sequence:

ATGCTTCGGT GGAGAATCCC AAAACAGCCA GACCCAGGAG CCAAGAGAAC AAGCGAGTCG 
TCCTCCACCC CGGGAAATGG GGCCACGCCC GAGGAGTGCC CGGCGCTGGC CGACAGCCCC 
ACCACGCTCA CCGAGGCCCT GCAGATGATC CACCCCATCC CCGCCGACTC CTGGAGAAAC 
CTCATCGAAC AAATAGGGCT CCTGTATCAG GAATACCGAG ATAAATCCAC GCTCCAAGAA 
ATTGAAACCA GGAGGCAGCA GGATGCAGAG ATACACACCA ATGCCCATGG CTCCCCGGCC 
AGCGAGGAGA CCCCAGAAGA GGAGGAGGAC GAGGAGGAGG AGGAGGACGA GCCGGCAAGC 
CCGCCCGAGA GGAAGGCTCT GCCCCAGATC TGCCTCCTCA GTAACCCCCA CTCGAGATTT 
AACCTCTGGC AGGACCTGCC CGAGATCCAG AGCAGCGGGG TGCTGGAAAT CCTCCAGCCC 
GAGGAGATCA AGCTGCAGGA GGCCATGTTC GAGCTGGTCA CTTCTGAGGC CTCCTACTAC 
AAAAGCCTGA GCCTGCTCGT GTCTCACTTC ATGGAGAATG AACGTCTGAA GAAGATCCTG 
CACCCGTCTG AGGCACACAT CCTCTTCTCC AATGTCCTGG ACGTCATGGC CGTCAGTGAA 
CGGTTTCTCC TGGAGCTGGA GCGCCGGATG GAGGAGAACA TCGTCATTTC GGACGTGTGC 
GACATCGTGT ACCATTACGC GGCCAACCAC TTCTCCGTGT ACATCACCTA CGTCAGCAAC 
CAGACCTACC AGGAGAGGAC CTACAAGCAG TTGCTCCAGG AGAAGGCGGC CTTTCGGGAG 
CTGATTGCGC AGTTGGAGCT GGACCCCAAG TGCAGGGGGC TGCCTCTGTC CTCCTTCCTC 
ATCCTGCCTT TCCAGAGGAT CACGCGCCTC AAGCTGTTGA TCCAGAACAT TCTGAAGAGA 
GTGGAAGAAA GGTCTGAGCG CGAGTGCACT GCTTTGGAGG CCCACAAGGA ACTGGAGATG 
GTGGTAAAAG CGTGCAACGA GGGTGTCAGG AAAATGAGCC GCACAGAGCA GATGATCAGC 
ATTCAGAAGA AGATGGAGTT TAAGATCAAG TCCGTGCCCA TCATCTCCCA CTCCCGCTGG 
CTGCTGAAGC AGGGTGAGCT GCAGCAGATG TCAGGCCCCA AGACCTCCCG AACCCTGCGG 
ACCAAGAAAC TCTTCCGGGA AATTTACCTC TTCCTCTTCA ACGACCTGCT GGTGATCTGC 
CGGCAGATCC CCGGAGACAA GTACCAAGTG TTTGACTCGG CCCCGAGGGG CCTCCTCCGG 
GTGGAGGAGC TGGAGGACCA GGGCCAGACG CTGGCCAACG TGTTCATCCT GCGGCTGCTG 
GAGAACGCGG ACGACCGGGA GGCCACCTAC ATGCTGAAGG CGTCTTCTCA GAGTGAGATG 
AAGCGTTGGA TGACCTCACT GGCCCCCAAC CGGAGAACCA AGTTTGTTTC CTTCACTTCC 
CGGCTGCTGG ACTGTCCCCA GGTCCAGTGT GTGCACCCGT ATGTGGCCCA GCAGCCAGAC 
GAGCTGACAC TGGAGCTGGC TGACATTCTG AACATTCTGG AAAAGACCGA AGACGGGTGG 
ATCTTTGGCG AGCGTCTGCA TGACCAGGAG AGAGGCTGGT TCCCCAGCTC CATGACTGAG 
GAGATCTTGA ATCCCAAGAT CCGGTCCCAG AACCTCAAGG AATGTTTCCG AGTCCACAAG 
ATGGACGACC CTCAGCGCAG CCAGAACAAG GATCGCAGGA AGCTGGGCAG CCGAAATCGG 
CAATGA