ATG4A cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATG4A
Entrez Gene ID	481015
Full Name	autophagy related 4A cysteine peptidase
General protein information	Preferred Names autophagy related 4A cysteine peptidase Names cysteine protease ATG4A ATG4 autophagy related 4 homolog A
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_538136.5	XP_538136.3	cysteine protease ATG4A
XM_538136.6	XP_538136.4	cysteine protease ATG4A

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ATG4A	NP_001258915.1
Danio rerio (zebrafish)	atg4a	NP_001019605.1
Drosophila melanogaster (fruit fly)	Atg4	NP_608563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008497	XP_316946.4
Caenorhabditis elegans (roundworm)	atg-4.1	NP_493375.2
Homo sapiens (human)	ATG4A	NP_443168.2
Pan troglodytes (chimpanzee)	ATG4A	NP_001233457.1
	ATG4A	XP_001097251.1
Oryza sativa (rice)	Os04g0682000	NP_001054293.1
Bos taurus (cattle)	ATG4A	NP_001001171.1
Rattus norvegicus (Norway rat)	Atg4al1	NP_001119770.1
Saccharomyces cerevisiae (baker's yeast)	ATG4	NP_014176.2
Kluyveromyces lactis	KLLA0D19536g	XP_453929.1
Eremothecium gossypii	AGOS_ABL191W	NP_982756.1
Arabidopsis thaliana (thale cress)	AT2G44140	NP_850412.1
Arabidopsis thaliana (thale cress)	AT3G59950	NP_191554.1
Xenopus tropicalis (tropical clawed frog)	atg4a	NP_001016619.1
Canis lupus familiaris (dog)	ATG4A	XP_538136.3
Mus musculus (house mouse)	Atg4a	NP_777364.3

Gene Ontology

The following ATG4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATG4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe32749	XM_538136.5 Latest version!	Canis lupus familiaris autophagy related 4A, cysteine peptidase (ATG4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe285202	XM_538136.6 Latest version!	Canis lupus familiaris autophagy related 4A cysteine peptidase (ATG4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATG4A ( XM_538136.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_538136.3 Canis lupus familiaris autophagy related 4A, cysteine peptidase (ATG4A), mRNA.

Price & Availability↑

CloneID	OCe32749
Clone ID Related Accession (Same CDS sequence)	XM_538136.5
Accession Version	XM_538136.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cysteine protease ATG4A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_538136.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAG TTTTATCCAA GTATGAAAAC CAGATTACTA TTTTTGCTGA CTACCTAGAA 
GAATTTCCAG ATTCAGATGA GCTGGTATGG ATCTTGGGGA AACAGCATCT CCTCAAAACG 
GAAAAATCTA AGCTGTTGTC TGATATAAGA GCTCGTCTAT GGTTTACATA CAGAAGGAAA 
TTTTCACCAA TTGGAGGAAC AGGCCCTTCA TCAGATGCTG GCTGGGGATG TATGCTACGC 
TGTGGACAAA TGATGCTGGC CCAGGCGCTT ATCTGCAGAC ACTTGGGAAG GGACTGGAGC 
TGGGAGAAAC AGAAAGAACA ACCCAGAGAA TACCAACGGA TCCTCCAGTG CTTCTTAGAT 
AGAAAGGACT GTTGCTACTC TATCCATCAA ATGGCACAGA TGGGTGTAGG AGAAGGGAAA 
TCAATTGGCG AATGGTTTGG ACCAAATACA GTTGCACAGG TATTAAAAAA ACTTGCTTTG 
TTTGACGAGT GGAATTCCTT GGCTATTTAT GTTTCGATGG ATAACACTGT GGTCATTGAA 
GATATCAAAA AAATGTGCTG TGTCCTCCCC TTGAGTGCAG ACACAATTGG TGAGAGCCCC 
CTCAACACTC TGAATGCTTC AAACCAGAGT AAGAGTGCCC CTGCCTCCTG CCCAGCCTGG 
AAACCCCTGC TGCTCATCGT GCCCCTTCGC CTGGGCATAA ACCAAATCAA CCCTGTCTAT 
GTTGATGCAT TCAAAGAGTG TTTTAAGATG CCACAGTCTT TAGGGGCATT AGGAGGAAAA 
CCAAATAACG CCTATTATTT CATAGGATTC TTAGGCGATG AGCTCATTTT CTTGGACCCT 
CATACAACCC AGACTTTTGT TGACACCGAA GAGAATGGAA CGGTTGATGA CCAGACTTTC 
CATTGCCTGC AGTCCCCACA GCGAATGAAC ATCTTGAACT TGGATCCTTC CGTCGCCCTG 
GGATTTTTCT GCAAAGAAGA AAAGGACTTT GATAGCTGGT GTAGCCTCGT GCAGAAGGAA 
ATTCTAAAGG AAAATTTAAG GATGTTTGAA CTGGTTCAGA AACATCCATC GCACTGGCCT 
CCCTTTGTTC CACCAGCCAA GCCAGAAGTG ACAACCACTG GGGCAGAATT CATTGACTCC 
ACCGAACAAC TAGAGGACTT CGACCTAGAG GAAGATTTTG AGATCCTGAG CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_538136.3
CDS	146..1342
Translation	MESVLSKYENQITIFADYLEEFPDSDELVWILGKQHLLKTEKSKLLSDIRARLWFTYRRKFSPIGGTGPSSDAGWGCMLRCGQMMLAQALICRHLGRDWSWEKQKEQPREYQRILQCFLDRKDCCYSIHQMAQMGVGEGKSIGEWFGPNTVAQVLKKLALFDEWNSLAIYVSMDNTVVIEDIKKMCCVLPLSADTIGESPLNTLNASNQSKSAPASCPAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALGGKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEENGTVDDQTFHCLQSPQRMNILNLDPSVALGFFCKEEKDFDSWCSLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEDFDLEEDFEILSV

Target ORF information:

RefSeq Version	XM_538136.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris autophagy related 4A, cysteine peptidase (ATG4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_538136.5

ORF Insert Sequence:

ATGGAGTCAG TTTTATCCAA GTATGAAAAC CAGATTACTA TTTTTGCTGA CTACCTAGAA 
GAATTTCCAG ATTCAGATGA GCTGGTATGG ATCTTGGGGA AACAGCATCT CCTCAAAACG 
GAAAAATCTA AGCTGTTGTC TGATATAAGA GCTCGTCTAT GGTTTACATA CAGAAGGAAA 
TTTTCACCAA TTGGAGGAAC AGGCCCTTCA TCAGATGCTG GCTGGGGATG TATGCTACGC 
TGTGGACAAA TGATGCTGGC CCAGGCGCTT ATCTGCAGAC ACTTGGGAAG GGACTGGAGC 
TGGGAGAAAC AGAAAGAACA ACCCAGAGAA TACCAACGGA TCCTCCAGTG CTTCTTAGAT 
AGAAAGGACT GTTGCTACTC TATCCATCAA ATGGCACAGA TGGGTGTAGG AGAAGGGAAA 
TCAATTGGCG AATGGTTTGG ACCAAATACA GTTGCACAGG TATTAAAAAA ACTTGCTTTG 
TTTGACGAGT GGAATTCCTT GGCTATTTAT GTTTCGATGG ATAACACTGT GGTCATTGAA 
GATATCAAAA AAATGTGCTG TGTCCTCCCC TTGAGTGCAG ACACAATTGG TGAGAGCCCC 
CTCAACACTC TGAATGCTTC AAACCAGAGT AAGAGTGCCC CTGCCTCCTG CCCAGCCTGG 
AAACCCCTGC TGCTCATCGT GCCCCTTCGC CTGGGCATAA ACCAAATCAA CCCTGTCTAT 
GTTGATGCAT TCAAAGAGTG TTTTAAGATG CCACAGTCTT TAGGGGCATT AGGAGGAAAA 
CCAAATAACG CCTATTATTT CATAGGATTC TTAGGCGATG AGCTCATTTT CTTGGACCCT 
CATACAACCC AGACTTTTGT TGACACCGAA GAGAATGGAA CGGTTGATGA CCAGACTTTC 
CATTGCCTGC AGTCCCCACA GCGAATGAAC ATCTTGAACT TGGATCCTTC CGTCGCCCTG 
GGATTTTTCT GCAAAGAAGA AAAGGACTTT GATAGCTGGT GTAGCCTCGT GCAGAAGGAA 
ATTCTAAAGG AAAATTTAAG GATGTTTGAA CTGGTTCAGA AACATCCATC GCACTGGCCT 
CCCTTTGTTC CACCAGCCAA GCCAGAAGTG ACAACCACTG GGGCAGAATT CATTGACTCC 
ACCGAACAAC TAGAGGACTT CGACCTAGAG GAAGATTTTG AGATCCTGAG CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG4A ( XM_538136.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_538136.4 Canis lupus familiaris autophagy related 4A cysteine peptidase (ATG4A), mRNA.

Price & Availability↑

CloneID	OCe285202
Clone ID Related Accession (Same CDS sequence)	XM_538136.6
Accession Version	XM_538136.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cysteine protease ATG4A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_538136.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCA CTGGATCCTC AGGGCGCCTC TGGGAGTTGC AGTTTGAGCG TAGTTCCGGG 
TGGAGAGGCG CCTTTACCGT TCTCACAGAC TCTGCCCCTA GCAGCGCGGA ACTACAGGTC 
CCAGGGTCCC TTGCGGCCGC CGTAGTCAAG TGCCGGGTGG AATTGGCCCA GGATGACAGC 
TGGAGAATGG AGTCAGTTTT ATCCAAGTAT GAAAACCAGA TTACTATTTT TGCTGACTAC 
CTAGAAGAAT TTCCAGATTC AGATGAGCTG GTATGGATCT TGGGGAAACA GCATCTCCTC 
AAAACGGAAA AATCTAAGCT GTTGTCTGAT ATAAGAGCTC GTCTATGGTT TACATACAGA 
AGGAAATTTT CACCAATTGG AGGAACAGGC CCTTCATCAG ATGCTGGCTG GGGATGTATG 
CTACGCTGTG GACAAATGAT GCTGGCCCAG GCGCTTATCT GCAGACACTT GGGAAGGGAC 
TGGAGCTGGG AGAAACAGAA AGAACAACCC AGAGAATACC AACGGATCCT CCAGTGCTTC 
TTAGATAGAA AGGACTGTTG CTACTCTATC CATCAAATGG CACAGATGGG TGTAGGAGAA 
GGGAAATCAA TTGGCGAATG GTTTGGACCA AATACAGTTG CACAGGTATT AAAAAAACTT 
GCTTTGTTTG ACGAGTGGAA TTCCTTGGCT ATTTATGTTT CGATGGATAA CACTGTGGTC 
ATTGAAGATA TCAAAAAAAT GTGCTGTGTC CTCCCCTTGA GTGCAGACAC AATTGGTGAG 
AGCCCCCTCA ACACTCTGAA TGCTTCAAAC CAGAGTAAGA GTGCCCCTGC CTCCTGCCCA 
GCCTGGAAAC CCCTGCTGCT CATCGTGCCC CTTCGCCTGG GCATAAACCA AATCAACCCT 
GTCTATGTTG ATGCATTCAA AGAGTGTTTT AAGATGCCAC AGTCTTTAGG GGCATTAGGA 
GGAAAACCAA ATAACGCCTA TTATTTCATA GGATTCTTAG GCGATGAGCT CATTTTCTTG 
GACCCTCATA CAACCCAGAC TTTTGTTGAC ACCGAAGAGA ATGGAACGGT TGATGACCAG 
ACTTTCCATT GCCTGCAGTC CCCACAGCGA ATGAACATCT TGAACTTGGA TCCTTCCGTC 
GCCCTGGGAT TTTTCTGCAA AGAAGAAAAG GACTTTGATA GCTGGTGTAG CCTCGTGCAG 
AAGGAAATTC TAAAGGAAAA TTTAAGGATG TTTGAACTGG TTCAGAAACA TCCATCGCAC 
TGGCCTCCCT TTGTTCCACC AGCCAAGCCA GAAGTGACAA CCACTGGGGC AGAATTCATT 
GACTCCACCG AACAACTAGA GGACTTCGAC CTAGAGGAAG ATTTTGAGAT CCTGAGCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_538136.4
CDS	120..1502
Translation	MESTGSSGRLWELQFERSSGWRGAFTVLTDSAPSSAELQVPGSLAAAVVKCRVELAQDDSWRMESVLSKYENQITIFADYLEEFPDSDELVWILGKQHLLKTEKSKLLSDIRARLWFTYRRKFSPIGGTGPSSDAGWGCMLRCGQMMLAQALICRHLGRDWSWEKQKEQPREYQRILQCFLDRKDCCYSIHQMAQMGVGEGKSIGEWFGPNTVAQVLKKLALFDEWNSLAIYVSMDNTVVIEDIKKMCCVLPLSADTIGESPLNTLNASNQSKSAPASCPAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALGGKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEENGTVDDQTFHCLQSPQRMNILNLDPSVALGFFCKEEKDFDSWCSLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEDFDLEEDFEILSV

Target ORF information:

RefSeq Version	XM_538136.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris autophagy related 4A cysteine peptidase (ATG4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_538136.6

ORF Insert Sequence:

ATGGAGAGCA CTGGATCCTC AGGGCGCCTC TGGGAGTTGC AGTTTGAGCG TAGTTCCGGG 
TGGAGAGGCG CCTTTACCGT TCTCACAGAC TCTGCCCCTA GCAGCGCGGA ACTACAGGTC 
CCAGGGTCCC TTGCGGCCGC CGTAGTCAAG TGCCGGGTGG AATTGGCCCA GGATGACAGC 
TGGAGAATGG AGTCAGTTTT ATCCAAGTAT GAAAACCAGA TTACTATTTT TGCTGACTAC 
CTAGAAGAAT TTCCAGATTC AGATGAGCTG GTATGGATCT TGGGGAAACA GCATCTCCTC 
AAAACGGAAA AATCTAAGCT GTTGTCTGAT ATAAGAGCTC GTCTATGGTT TACATACAGA 
AGGAAATTTT CACCAATTGG AGGAACAGGC CCTTCATCAG ATGCTGGCTG GGGATGTATG 
CTACGCTGTG GACAAATGAT GCTGGCCCAG GCGCTTATCT GCAGACACTT GGGAAGGGAC 
TGGAGCTGGG AGAAACAGAA AGAACAACCC AGAGAATACC AACGGATCCT CCAGTGCTTC 
TTAGATAGAA AGGACTGTTG CTACTCTATC CATCAAATGG CACAGATGGG TGTAGGAGAA 
GGGAAATCAA TTGGCGAATG GTTTGGACCA AATACAGTTG CACAGGTATT AAAAAAACTT 
GCTTTGTTTG ACGAGTGGAA TTCCTTGGCT ATTTATGTTT CGATGGATAA CACTGTGGTC 
ATTGAAGATA TCAAAAAAAT GTGCTGTGTC CTCCCCTTGA GTGCAGACAC AATTGGTGAG 
AGCCCCCTCA ACACTCTGAA TGCTTCAAAC CAGAGTAAGA GTGCCCCTGC CTCCTGCCCA 
GCCTGGAAAC CCCTGCTGCT CATCGTGCCC CTTCGCCTGG GCATAAACCA AATCAACCCT 
GTCTATGTTG ATGCATTCAA AGAGTGTTTT AAGATGCCAC AGTCTTTAGG GGCATTAGGA 
GGAAAACCAA ATAACGCCTA TTATTTCATA GGATTCTTAG GCGATGAGCT CATTTTCTTG 
GACCCTCATA CAACCCAGAC TTTTGTTGAC ACCGAAGAGA ATGGAACGGT TGATGACCAG 
ACTTTCCATT GCCTGCAGTC CCCACAGCGA ATGAACATCT TGAACTTGGA TCCTTCCGTC 
GCCCTGGGAT TTTTCTGCAA AGAAGAAAAG GACTTTGATA GCTGGTGTAG CCTCGTGCAG 
AAGGAAATTC TAAAGGAAAA TTTAAGGATG TTTGAACTGG TTCAGAAACA TCCATCGCAC 
TGGCCTCCCT TTGTTCCACC AGCCAAGCCA GAAGTGACAA CCACTGGGGC AGAATTCATT 
GACTCCACCG AACAACTAGA GGACTTCGAC CTAGAGGAAG ATTTTGAGAT CCTGAGCGTC 
TAA