TSC1 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022423654.1	XP_022279362.1	hamartin isoform X1
XM_022423656.1	XP_022279364.1	hamartin isoform X2
XM_014116967.1	XP_013972442.1	hamartin isoform X1
XM_014116968.1	XP_013972443.1	hamartin isoform X1
XM_537808.5	XP_537808.3	hamartin isoform X2
XM_014116969.1	XP_013972444.1	hamartin isoform X3
XM_005625176.2	XP_005625233.1	hamartin isoform X1
XM_537808.6	XP_537808.3	hamartin isoform X1
XM_022423653.1	XP_022279361.1	hamartin isoform X1
XM_022423655.1	XP_022279363.1	hamartin isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe12639	XM_022423656.1 Latest version!	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe38636	XM_014116969.1 Latest version!	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe12638	XM_022423654.1 Latest version!	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe12638	XM_537808.6 Latest version!	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe12638	XM_022423655.1 Latest version!	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe12638	XM_022423653.1 Latest version!	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe12640	XM_014116968.1 Latest version!	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe12640	XM_014116967.1 Latest version!	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe12640	XM_005625176.2 Latest version!	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OCe12638	XM_537808.5	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

TSC1 ( XM_022423656.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279364.1 Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe12639
Clone ID Related Accession (Same CDS sequence)	XM_022423656.1
Accession Version	XM_022423656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hamartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGAT 
GAGATCCGAA CCCTCCGTAA CCAGTTGCTT TTACTGCACA ACCAGTTACT CTATGAGCGT 
TTTAAGAGGC AGCAGCATGC TCTCCGAAAC AGGCGGCTCC TCCGCAAGGT GATCAAGGCG 
GCAGCTCTGG AGGAGCACAA TGCTGCTATG AAAGACCAGT TGAAGTTACA GGAGAAGGAC 
ATCCAGATCT GGAAGGTTAG TCTACAGAAG GAACAAGCCC GGTACAGCCA GCTGCAGGAT 
CAGCGAGACA CGATGGTCAC CCAGCTCCAT AGCCAGATCC GACAGCTGCA GCATGACCGG 
GAGGAGTTCT ATAACCAGAG CCAGGAGCTG CAGACGAAGC TGGAGGACTG CAGGAACATG 
ATCGCAGAGC TGCGTCTGGA ATTGAAGAAG GCCAACAGCA AGGTGTGTCA CACCGAGCTG 
CTGCTCAGTC AGGTGTCCCA GAAGCTCTCA AACAGCGAGT CGGTCCAGCA GCAGATGGAG 
TTCTTGAATC GGCAGCTCTT GGTCCTGGGG GAGGTCAACG AGCTGTATCT GGAGCAACTG 
CAGAGCAAGC ACTCGGATAC CACGAAGGAG GTAGAGATGA TGAAAGCTGC GTGTCGGAAA 
GAATTAGAGA AAAACAGAAG CCACGTTCTC CAGCAGAATC AGAGGCTCGA TACCTCTCAG 
AAACGGATTT TGGAACTGGA ATCTCACTTG GCCAAGAAAG ACCACCTTCT TTTGGAGCAG 
AAGAAATATT TAGAGGACGT CAAAGTCCAG GCAAGAGGAC AGCTGCAGGC GGCAGAGAGC 
AGGTACGAGG CTCAGAAAAG GATCACCCAA GTGTTTGAAT TGGAGATCTT AGATTTATAC 
GGCAGGTTGG AGAAGGATGG GCTCCTGAAG AAACTTGAAG AAGAAAAAGC AGAAGCAGCT 
GAAGCAGCAG AAGAAAGGCT TGCCTGTTGT AAGGATGGCT GCTCAGATTC TGTAACAGAC 
CACCATGAAG AGGCATCTGG CCACAACGGC GAGACCAAGC CCCCCAGGCC TGGTGGTGCA 
CGGGGCAGCA GTGGAAGTAG AGGGGGTGGA GGTGGCAGCA GTGGCGGCAG CAGCAGCGAG 
CTTTCAACCC CAGAGAAACC CCCCAACCAG AGGGCAGGCC TGTTCAGCAG TCGGTGGGAA 
ACGACCGTGG GAGAGCCTGC CACCAGCATT CCCACTACTG TCGGCTCCCT TCCCAGTTCA 
AAAAGCTTCC TAGGCATGAA GGCACGAGAG TTATTTCGTA ATAAGAGTGA GAGCCAGTGT 
GATGAGGACA GTGTGACCAT CAGTAGCCTT TCTGAGACGC TAAAGACAGA ACTGGGCAAA 
GACCTGGGTG TGGAAGCCAA GACTCCCCCA ACCCTTGATG GCCCACACCC CACCTCCCCG 
AACCCAGACA GTGTTGGACA GCTCCACATC ATGGACTACA ATGAGACTCA TCATGAACAC 
AGTGAAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279364.1
CDS	302..3793
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_022423656.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423656.1

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGAT 
GAGATCCGAA CCCTCCGTAA CCAGTTGCTT TTACTGCACA ACCAGTTACT CTATGAGCGT 
TTTAAGAGGC AGCAGCATGC TCTCCGAAAC AGGCGGCTCC TCCGCAAGGT GATCAAGGCG 
GCAGCTCTGG AGGAGCACAA TGCTGCTATG AAAGACCAGT TGAAGTTACA GGAGAAGGAC 
ATCCAGATCT GGAAGGTTAG TCTACAGAAG GAACAAGCCC GGTACAGCCA GCTGCAGGAT 
CAGCGAGACA CGATGGTCAC CCAGCTCCAT AGCCAGATCC GACAGCTGCA GCATGACCGG 
GAGGAGTTCT ATAACCAGAG CCAGGAGCTG CAGACGAAGC TGGAGGACTG CAGGAACATG 
ATCGCAGAGC TGCGTCTGGA ATTGAAGAAG GCCAACAGCA AGGTGTGTCA CACCGAGCTG 
CTGCTCAGTC AGGTGTCCCA GAAGCTCTCA AACAGCGAGT CGGTCCAGCA GCAGATGGAG 
TTCTTGAATC GGCAGCTCTT GGTCCTGGGG GAGGTCAACG AGCTGTATCT GGAGCAACTG 
CAGAGCAAGC ACTCGGATAC CACGAAGGAG GTAGAGATGA TGAAAGCTGC GTGTCGGAAA 
GAATTAGAGA AAAACAGAAG CCACGTTCTC CAGCAGAATC AGAGGCTCGA TACCTCTCAG 
AAACGGATTT TGGAACTGGA ATCTCACTTG GCCAAGAAAG ACCACCTTCT TTTGGAGCAG 
AAGAAATATT TAGAGGACGT CAAAGTCCAG GCAAGAGGAC AGCTGCAGGC GGCAGAGAGC 
AGGTACGAGG CTCAGAAAAG GATCACCCAA GTGTTTGAAT TGGAGATCTT AGATTTATAC 
GGCAGGTTGG AGAAGGATGG GCTCCTGAAG AAACTTGAAG AAGAAAAAGC AGAAGCAGCT 
GAAGCAGCAG AAGAAAGGCT TGCCTGTTGT AAGGATGGCT GCTCAGATTC TGTAACAGAC 
CACCATGAAG AGGCATCTGG CCACAACGGC GAGACCAAGC CCCCCAGGCC TGGTGGTGCA 
CGGGGCAGCA GTGGAAGTAG AGGGGGTGGA GGTGGCAGCA GTGGCGGCAG CAGCAGCGAG 
CTTTCAACCC CAGAGAAACC CCCCAACCAG AGGGCAGGCC TGTTCAGCAG TCGGTGGGAA 
ACGACCGTGG GAGAGCCTGC CACCAGCATT CCCACTACTG TCGGCTCCCT TCCCAGTTCA 
AAAAGCTTCC TAGGCATGAA GGCACGAGAG TTATTTCGTA ATAAGAGTGA GAGCCAGTGT 
GATGAGGACA GTGTGACCAT CAGTAGCCTT TCTGAGACGC TAAAGACAGA ACTGGGCAAA 
GACCTGGGTG TGGAAGCCAA GACTCCCCCA ACCCTTGATG GCCCACACCC CACCTCCCCG 
AACCCAGACA GTGTTGGACA GCTCCACATC ATGGACTACA ATGAGACTCA TCATGAACAC 
AGTGAAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_014116969.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972444.1 Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe38636
Clone ID Related Accession (Same CDS sequence)	XM_014116969.1
Accession Version	XM_014116969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hamartin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACTT CTCCTCCTCC AGCCCCATTG 
TGCCACTCGG ATGACTATGT GCACATTTCC CTCCAGCAGG CCACAGTCCC AGCTCCCAAG 
AAGGAAGAGA AAACAGATTC AGCAAGGCCC TGTCTACACA GACAGCACCA TCTTCCAAAC 
GACAGAGGAT TAGAAGAGCT ACCTGGCCTC AAAGGTTCTG TCACTCTAAG TGATCTTCCA 
GGGTTTTTAG GGGACCTTGC CTCTGATGAA GATAGCGTTG AAAAAGACAA AGAAGAAGCG 
GCGATATCAA AAGAGCTCTC AGAGATCACG ACGGCTGACA CTGAGCCCCT GGTTCCTCGA 
GGAGGCTTTG ACTCTCCCTT CTACCCGGAC AGTCTCCCGG GCTGTCCTCG CAAGACCCAT 
TCAGCCACCT CTGGTCCTCA GGGTACCAGT GTGAATCCTG AACCTTTCAA CTCCTCCTTG 
GACAAGCGTG GGCCAGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGTGGA 
GGCACCCACG AAAGCCCTGC CGGGGACAGG GAGCGCCAGA CTTCGCTGGA GACCAACATC 
CTCACTCCCA GCCCTTGTAA AATTCCACCT CAGAGGGGAG TGGGGTTTGG AAGTGGGCAG 
CCTCCCCCTT ACGATCATCT TTTTGAGGTG GCATTGCCAA AGACCGCCCA TCATTTTGTC 
AGCAAGAAGA CCAAGGAACT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGACTACCCG 
CCCTCAACCT CCCCAGCAGA AGTTCTGGAT AGGCTGATCC AGCAGGGAGC CGACGCGCAC 
AGCAAGGAGC TGAGTCGGTT GTCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA 
GATGAGATCC GAACCCTCCG TAACCAGTTG CTTTTACTGC ACAACCAGTT ACTCTATGAG 
CGTTTTAAGA GGCAGCAGCA TGCTCTCCGA AACAGGCGGC TCCTCCGCAA GGTGATCAAG 
GCGGCAGCTC TGGAGGAGCA CAATGCTGCT ATGAAAGACC AGTTGAAGTT ACAGGAGAAG 
GACATCCAGA TCTGGAAGGT TAGTCTACAG AAGGAACAAG CCCGGTACAG CCAGCTGCAG 
GATCAGCGAG ACACGATGGT CACCCAGCTC CATAGCCAGA TCCGACAGCT GCAGCATGAC 
CGGGAGGAGT TCTATAACCA GAGCCAGGAG CTGCAGACGA AGCTGGAGGA CTGCAGGAAC 
ATGATCGCAG AGCTGCGTCT GGAATTGAAG AAGGCCAACA GCAAGGTGTG TCACACCGAG 
CTGCTGCTCA GTCAGGTGTC CCAGAAGCTC TCAAACAGCG AGTCGGTCCA GCAGCAGATG 
GAGTTCTTGA ATCGGCAGCT CTTGGTCCTG GGGGAGGTCA ACGAGCTGTA TCTGGAGCAA 
CTGCAGAGCA AGCACTCGGA TACCACGAAG GAGGTAGAGA TGATGAAAGC TGCGTGTCGG 
AAAGAATTAG AGAAAAACAG AAGCCACGTT CTCCAGCAGA ATCAGAGGCT CGATACCTCT 
CAGAAACGGA TTTTGGAACT GGAATCTCAC TTGGCCAAGA AAGACCACCT TCTTTTGGAG 
CAGAAGAAAT ATTTAGAGGA CGTCAAAGTC CAGGCAAGAG GACAGCTGCA GGCGGCAGAG 
AGCAGGTACG AGGCTCAGAA AAGGATCACC CAAGTGTTTG AATTGGAGAT CTTAGATTTA 
TACGGCAGGT TGGAGAAGGA TGGGCTCCTG AAGAAACTTG AAGAAGAAAA AGCAGAAGCA 
GCTGAAGCAG CAGAAGAAAG GCTTGCCTGT TGTAAGGATG GCTGCTCAGA TTCTGTAACA 
GACCACCATG AAGAGGCATC TGGCCACAAC GGCGAGACCA AGCCCCCCAG GCCTGGTGGT 
GCACGGGGCA GCAGTGGAAG TAGAGGGGGT GGAGGTGGCA GCAGTGGCGG CAGCAGCAGC 
GAGCTTTCAA CCCCAGAGAA ACCCCCCAAC CAGAGGGCAG GCCTGTTCAG CAGTCGGTGG 
GAAACGACCG TGGGAGAGCC TGCCACCAGC ATTCCCACTA CTGTCGGCTC CCTTCCCAGT 
TCAAAAAGCT TCCTAGGCAT GAAGGCACGA GAGTTATTTC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ACAGTGTGAC CATCAGTAGC CTTTCTGAGA CGCTAAAGAC AGAACTGGGC 
AAAGACCTGG GTGTGGAAGC CAAGACTCCC CCAACCCTTG ATGGCCCACA CCCCACCTCC 
CCGAACCCAG ACAGTGTTGG ACAGCTCCAC ATCATGGACT ACAATGAGAC TCATCATGAA 
CACAGTGAAG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972444.1
CDS	495..3989
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_014116969.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116969.1

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACTT CTCCTCCTCC AGCCCCATTG 
TGCCACTCGG ATGACTATGT GCACATTTCC CTCCAGCAGG CCACAGTCCC AGCTCCCAAG 
AAGGAAGAGA AAACAGATTC AGCAAGGCCC TGTCTACACA GACAGCACCA TCTTCCAAAC 
GACAGAGGAT TAGAAGAGCT ACCTGGCCTC AAAGGTTCTG TCACTCTAAG TGATCTTCCA 
GGGTTTTTAG GGGACCTTGC CTCTGATGAA GATAGCGTTG AAAAAGACAA AGAAGAAGCG 
GCGATATCAA AAGAGCTCTC AGAGATCACG ACGGCTGACA CTGAGCCCCT GGTTCCTCGA 
GGAGGCTTTG ACTCTCCCTT CTACCCGGAC AGTCTCCCGG GCTGTCCTCG CAAGACCCAT 
TCAGCCACCT CTGGTCCTCA GGGTACCAGT GTGAATCCTG AACCTTTCAA CTCCTCCTTG 
GACAAGCGTG GGCCAGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGTGGA 
GGCACCCACG AAAGCCCTGC CGGGGACAGG GAGCGCCAGA CTTCGCTGGA GACCAACATC 
CTCACTCCCA GCCCTTGTAA AATTCCACCT CAGAGGGGAG TGGGGTTTGG AAGTGGGCAG 
CCTCCCCCTT ACGATCATCT TTTTGAGGTG GCATTGCCAA AGACCGCCCA TCATTTTGTC 
AGCAAGAAGA CCAAGGAACT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGACTACCCG 
CCCTCAACCT CCCCAGCAGA AGTTCTGGAT AGGCTGATCC AGCAGGGAGC CGACGCGCAC 
AGCAAGGAGC TGAGTCGGTT GTCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA 
GATGAGATCC GAACCCTCCG TAACCAGTTG CTTTTACTGC ACAACCAGTT ACTCTATGAG 
CGTTTTAAGA GGCAGCAGCA TGCTCTCCGA AACAGGCGGC TCCTCCGCAA GGTGATCAAG 
GCGGCAGCTC TGGAGGAGCA CAATGCTGCT ATGAAAGACC AGTTGAAGTT ACAGGAGAAG 
GACATCCAGA TCTGGAAGGT TAGTCTACAG AAGGAACAAG CCCGGTACAG CCAGCTGCAG 
GATCAGCGAG ACACGATGGT CACCCAGCTC CATAGCCAGA TCCGACAGCT GCAGCATGAC 
CGGGAGGAGT TCTATAACCA GAGCCAGGAG CTGCAGACGA AGCTGGAGGA CTGCAGGAAC 
ATGATCGCAG AGCTGCGTCT GGAATTGAAG AAGGCCAACA GCAAGGTGTG TCACACCGAG 
CTGCTGCTCA GTCAGGTGTC CCAGAAGCTC TCAAACAGCG AGTCGGTCCA GCAGCAGATG 
GAGTTCTTGA ATCGGCAGCT CTTGGTCCTG GGGGAGGTCA ACGAGCTGTA TCTGGAGCAA 
CTGCAGAGCA AGCACTCGGA TACCACGAAG GAGGTAGAGA TGATGAAAGC TGCGTGTCGG 
AAAGAATTAG AGAAAAACAG AAGCCACGTT CTCCAGCAGA ATCAGAGGCT CGATACCTCT 
CAGAAACGGA TTTTGGAACT GGAATCTCAC TTGGCCAAGA AAGACCACCT TCTTTTGGAG 
CAGAAGAAAT ATTTAGAGGA CGTCAAAGTC CAGGCAAGAG GACAGCTGCA GGCGGCAGAG 
AGCAGGTACG AGGCTCAGAA AAGGATCACC CAAGTGTTTG AATTGGAGAT CTTAGATTTA 
TACGGCAGGT TGGAGAAGGA TGGGCTCCTG AAGAAACTTG AAGAAGAAAA AGCAGAAGCA 
GCTGAAGCAG CAGAAGAAAG GCTTGCCTGT TGTAAGGATG GCTGCTCAGA TTCTGTAACA 
GACCACCATG AAGAGGCATC TGGCCACAAC GGCGAGACCA AGCCCCCCAG GCCTGGTGGT 
GCACGGGGCA GCAGTGGAAG TAGAGGGGGT GGAGGTGGCA GCAGTGGCGG CAGCAGCAGC 
GAGCTTTCAA CCCCAGAGAA ACCCCCCAAC CAGAGGGCAG GCCTGTTCAG CAGTCGGTGG 
GAAACGACCG TGGGAGAGCC TGCCACCAGC ATTCCCACTA CTGTCGGCTC CCTTCCCAGT 
TCAAAAAGCT TCCTAGGCAT GAAGGCACGA GAGTTATTTC GTAATAAGAG TGAGAGCCAG 
TGTGATGAGG ACAGTGTGAC CATCAGTAGC CTTTCTGAGA CGCTAAAGAC AGAACTGGGC 
AAAGACCTGG GTGTGGAAGC CAAGACTCCC CCAACCCTTG ATGGCCCACA CCCCACCTCC 
CCGAACCCAG ACAGTGTTGG ACAGCTCCAC ATCATGGACT ACAATGAGAC TCATCATGAA 
CACAGTGAAG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_022423654.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279362.1 Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.

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CloneID	OCe12638
Clone ID Related Accession (Same CDS sequence)	XM_022423654.1 , XM_537808.5 , XM_537808.6 , XM_022423653.1 , XM_022423655.1
Accession Version	XM_022423654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279362.1
CDS	261..3767
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_022423654.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423654.1

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_537808.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537808.3 Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe12638
Clone ID Related Accession (Same CDS sequence)	XM_022423654.1 , XM_537808.5 , XM_537808.6 , XM_022423653.1 , XM_022423655.1
Accession Version	XM_537808.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hamartin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537808.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537808.3
CDS	494..4000
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_537808.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537808.5

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_537808.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537808.3 Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

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CloneID	OCe12638
Clone ID Related Accession (Same CDS sequence)	XM_022423654.1 , XM_537808.5 , XM_537808.6 , XM_022423653.1 , XM_022423655.1
Accession Version	XM_537808.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537808.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537808.3
CDS	302..3808
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_537808.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537808.6

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_022423653.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279361.1 Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

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CloneID	OCe12638
Clone ID Related Accession (Same CDS sequence)	XM_022423654.1 , XM_537808.5 , XM_537808.6 , XM_022423653.1 , XM_022423655.1
Accession Version	XM_022423653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279361.1
CDS	283..3789
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_022423653.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423653.1

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_022423655.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022279363.1 Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe12638
Clone ID Related Accession (Same CDS sequence)	XM_022423654.1 , XM_537808.5 , XM_537808.6 , XM_022423653.1 , XM_022423655.1
Accession Version	XM_022423655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	hamartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022279363.1
CDS	378..3884
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_022423655.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TSC complex subunit 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022423655.1

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACTTCTC CTCCTCCAGC CCCATTGTGC 
CACTCGGATG ACTATGTGCA CATTTCCCTC CAGCAGGCCA CAGTCCCAGC TCCCAAGAAG 
GAAGAGAAAA CAGATTCAGC AAGGCCCTGT CTACACAGAC AGCACCATCT TCCAAACGAC 
AGAGGATTAG AAGAGCTACC TGGCCTCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG 
TTTTTAGGGG ACCTTGCCTC TGATGAAGAT AGCGTTGAAA AAGACAAAGA AGAAGCGGCG 
ATATCAAAAG AGCTCTCAGA GATCACGACG GCTGACACTG AGCCCCTGGT TCCTCGAGGA 
GGCTTTGACT CTCCCTTCTA CCCGGACAGT CTCCCGGGCT GTCCTCGCAA GACCCATTCA 
GCCACCTCTG GTCCTCAGGG TACCAGTGTG AATCCTGAAC CTTTCAACTC CTCCTTGGAC 
AAGCGTGGGC CAGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGTGGAGGC 
ACCCACGAAA GCCCTGCCGG GGACAGGGAG CGCCAGACTT CGCTGGAGAC CAACATCCTC 
ACTCCCAGCC CTTGTAAAAT TCCACCTCAG AGGGGAGTGG GGTTTGGAAG TGGGCAGCCT 
CCCCCTTACG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CCGCCCATCA TTTTGTCAGC 
AAGAAGACCA AGGAACTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA CTACCCGCCC 
TCAACCTCCC CAGCAGAAGT TCTGGATAGG CTGATCCAGC AGGGAGCCGA CGCGCACAGC 
AAGGAGCTGA GTCGGTTGTC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC 
TCTCCTCCCT CAGATGAGAT CCGAACCCTC CGTAACCAGT TGCTTTTACT GCACAACCAG 
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCTCTCC GAAACAGGCG GCTCCTCCGC 
AAGGTGATCA AGGCGGCAGC TCTGGAGGAG CACAATGCTG CTATGAAAGA CCAGTTGAAG 
TTACAGGAGA AGGACATCCA GATCTGGAAG GTTAGTCTAC AGAAGGAACA AGCCCGGTAC 
AGCCAGCTGC AGGATCAGCG AGACACGATG GTCACCCAGC TCCATAGCCA GATCCGACAG 
CTGCAGCATG ACCGGGAGGA GTTCTATAAC CAGAGCCAGG AGCTGCAGAC GAAGCTGGAG 
GACTGCAGGA ACATGATCGC AGAGCTGCGT CTGGAATTGA AGAAGGCCAA CAGCAAGGTG 
TGTCACACCG AGCTGCTGCT CAGTCAGGTG TCCCAGAAGC TCTCAAACAG CGAGTCGGTC 
CAGCAGCAGA TGGAGTTCTT GAATCGGCAG CTCTTGGTCC TGGGGGAGGT CAACGAGCTG 
TATCTGGAGC AACTGCAGAG CAAGCACTCG GATACCACGA AGGAGGTAGA GATGATGAAA 
GCTGCGTGTC GGAAAGAATT AGAGAAAAAC AGAAGCCACG TTCTCCAGCA GAATCAGAGG 
CTCGATACCT CTCAGAAACG GATTTTGGAA CTGGAATCTC ACTTGGCCAA GAAAGACCAC 
CTTCTTTTGG AGCAGAAGAA ATATTTAGAG GACGTCAAAG TCCAGGCAAG AGGACAGCTG 
CAGGCGGCAG AGAGCAGGTA CGAGGCTCAG AAAAGGATCA CCCAAGTGTT TGAATTGGAG 
ATCTTAGATT TATACGGCAG GTTGGAGAAG GATGGGCTCC TGAAGAAACT TGAAGAAGAA 
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGCCT GTTGTAAGGA TGGCTGCTCA 
GATTCTGTAA CAGACCACCA TGAAGAGGCA TCTGGCCACA ACGGCGAGAC CAAGCCCCCC 
AGGCCTGGTG GTGCACGGGG CAGCAGTGGA AGTAGAGGGG GTGGAGGTGG CAGCAGTGGC 
GGCAGCAGCA GCGAGCTTTC AACCCCAGAG AAACCCCCCA ACCAGAGGGC AGGCCTGTTC 
AGCAGTCGGT GGGAAACGAC CGTGGGAGAG CCTGCCACCA GCATTCCCAC TACTGTCGGC 
TCCCTTCCCA GTTCAAAAAG CTTCCTAGGC ATGAAGGCAC GAGAGTTATT TCGTAATAAG 
AGTGAGAGCC AGTGTGATGA GGACAGTGTG ACCATCAGTA GCCTTTCTGA GACGCTAAAG 
ACAGAACTGG GCAAAGACCT GGGTGTGGAA GCCAAGACTC CCCCAACCCT TGATGGCCCA 
CACCCCACCT CCCCGAACCC AGACAGTGTT GGACAGCTCC ACATCATGGA CTACAATGAG 
ACTCATCATG AACACAGTGA AGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_014116967.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972442.1 Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

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CloneID	OCe12640
Clone ID Related Accession (Same CDS sequence)	XM_014116967.1 , XM_014116968.1 , XM_005625176.2
Accession Version	XM_014116967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hamartin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACTT CTCCTCCTCC AGCCCCATTG 
TGCCACTCGG ATGACTATGT GCACATTTCC CTCCAGCAGG CCACAGTCCC AGCTCCCAAG 
AAGGAAGAGA AAACAGATTC AGCAAGGCCC TGTCTACACA GACAGCACCA TCTTCCAAAC 
GACAGAGGAT TAGAAGAGCT ACCTGGCCTC AAAGGTTCTG TCACTCTAAG TGATCTTCCA 
GGGTTTTTAG GGGACCTTGC CTCTGATGAA GATAGCGTTG AAAAAGACAA AGAAGAAGCG 
GCGATATCAA AAGAGCTCTC AGAGATCACG ACGGCTGACA CTGAGCCCCT GGTTCCTCGA 
GGAGGCTTTG ACTCTCCCTT CTACCCGGAC AGTCTCCCGG GCTGTCCTCG CAAGACCCAT 
TCAGCCACCT CTGGTCCTCA GGGTACCAGT GTGAATCCTG AACCTTTCAA CTCCTCCTTG 
GACAAGCGTG GGCCAGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGTGGA 
GGCACCCACG AAAGCCCTGC CGGGGACAGG GAGCGCCAGA CTTCGCTGGA GACCAACATC 
CTCACTCCCA GCCCTTGTAA AATTCCACCT CAGAGGGGAG TGGGGTTTGG AAGTGGGCAG 
CCTCCCCCTT ACGATCATCT TTTTGAGGTG GCATTGCCAA AGACCGCCCA TCATTTTGTC 
AGCAAGAAGA CCAAGGAACT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGACTACCCG 
CCCTCAACCT CCCCAGCAGA AGTTCTGGAT AGGCTGATCC AGCAGGGAGC CGACGCGCAC 
AGCAAGGAGC TGAGTCGGTT GTCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA 
GGCTCTCCTC CCTCAGATGA GATCCGAACC CTCCGTAACC AGTTGCTTTT ACTGCACAAC 
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCTC TCCGAAACAG GCGGCTCCTC 
CGCAAGGTGA TCAAGGCGGC AGCTCTGGAG GAGCACAATG CTGCTATGAA AGACCAGTTG 
AAGTTACAGG AGAAGGACAT CCAGATCTGG AAGGTTAGTC TACAGAAGGA ACAAGCCCGG 
TACAGCCAGC TGCAGGATCA GCGAGACACG ATGGTCACCC AGCTCCATAG CCAGATCCGA 
CAGCTGCAGC ATGACCGGGA GGAGTTCTAT AACCAGAGCC AGGAGCTGCA GACGAAGCTG 
GAGGACTGCA GGAACATGAT CGCAGAGCTG CGTCTGGAAT TGAAGAAGGC CAACAGCAAG 
GTGTGTCACA CCGAGCTGCT GCTCAGTCAG GTGTCCCAGA AGCTCTCAAA CAGCGAGTCG 
GTCCAGCAGC AGATGGAGTT CTTGAATCGG CAGCTCTTGG TCCTGGGGGA GGTCAACGAG 
CTGTATCTGG AGCAACTGCA GAGCAAGCAC TCGGATACCA CGAAGGAGGT AGAGATGATG 
AAAGCTGCGT GTCGGAAAGA ATTAGAGAAA AACAGAAGCC ACGTTCTCCA GCAGAATCAG 
AGGCTCGATA CCTCTCAGAA ACGGATTTTG GAACTGGAAT CTCACTTGGC CAAGAAAGAC 
CACCTTCTTT TGGAGCAGAA GAAATATTTA GAGGACGTCA AAGTCCAGGC AAGAGGACAG 
CTGCAGGCGG CAGAGAGCAG GTACGAGGCT CAGAAAAGGA TCACCCAAGT GTTTGAATTG 
GAGATCTTAG ATTTATACGG CAGGTTGGAG AAGGATGGGC TCCTGAAGAA ACTTGAAGAA 
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG CCTGTTGTAA GGATGGCTGC 
TCAGATTCTG TAACAGACCA CCATGAAGAG GCATCTGGCC ACAACGGCGA GACCAAGCCC 
CCCAGGCCTG GTGGTGCACG GGGCAGCAGT GGAAGTAGAG GGGGTGGAGG TGGCAGCAGT 
GGCGGCAGCA GCAGCGAGCT TTCAACCCCA GAGAAACCCC CCAACCAGAG GGCAGGCCTG 
TTCAGCAGTC GGTGGGAAAC GACCGTGGGA GAGCCTGCCA CCAGCATTCC CACTACTGTC 
GGCTCCCTTC CCAGTTCAAA AAGCTTCCTA GGCATGAAGG CACGAGAGTT ATTTCGTAAT 
AAGAGTGAGA GCCAGTGTGA TGAGGACAGT GTGACCATCA GTAGCCTTTC TGAGACGCTA 
AAGACAGAAC TGGGCAAAGA CCTGGGTGTG GAAGCCAAGA CTCCCCCAAC CCTTGATGGC 
CCACACCCCA CCTCCCCGAA CCCAGACAGT GTTGGACAGC TCCACATCAT GGACTACAAT 
GAGACTCATC ATGAACACAG TGAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972442.1
CDS	466..3975
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_014116967.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116967.1

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACTT CTCCTCCTCC AGCCCCATTG 
TGCCACTCGG ATGACTATGT GCACATTTCC CTCCAGCAGG CCACAGTCCC AGCTCCCAAG 
AAGGAAGAGA AAACAGATTC AGCAAGGCCC TGTCTACACA GACAGCACCA TCTTCCAAAC 
GACAGAGGAT TAGAAGAGCT ACCTGGCCTC AAAGGTTCTG TCACTCTAAG TGATCTTCCA 
GGGTTTTTAG GGGACCTTGC CTCTGATGAA GATAGCGTTG AAAAAGACAA AGAAGAAGCG 
GCGATATCAA AAGAGCTCTC AGAGATCACG ACGGCTGACA CTGAGCCCCT GGTTCCTCGA 
GGAGGCTTTG ACTCTCCCTT CTACCCGGAC AGTCTCCCGG GCTGTCCTCG CAAGACCCAT 
TCAGCCACCT CTGGTCCTCA GGGTACCAGT GTGAATCCTG AACCTTTCAA CTCCTCCTTG 
GACAAGCGTG GGCCAGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGTGGA 
GGCACCCACG AAAGCCCTGC CGGGGACAGG GAGCGCCAGA CTTCGCTGGA GACCAACATC 
CTCACTCCCA GCCCTTGTAA AATTCCACCT CAGAGGGGAG TGGGGTTTGG AAGTGGGCAG 
CCTCCCCCTT ACGATCATCT TTTTGAGGTG GCATTGCCAA AGACCGCCCA TCATTTTGTC 
AGCAAGAAGA CCAAGGAACT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGACTACCCG 
CCCTCAACCT CCCCAGCAGA AGTTCTGGAT AGGCTGATCC AGCAGGGAGC CGACGCGCAC 
AGCAAGGAGC TGAGTCGGTT GTCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA 
GGCTCTCCTC CCTCAGATGA GATCCGAACC CTCCGTAACC AGTTGCTTTT ACTGCACAAC 
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCTC TCCGAAACAG GCGGCTCCTC 
CGCAAGGTGA TCAAGGCGGC AGCTCTGGAG GAGCACAATG CTGCTATGAA AGACCAGTTG 
AAGTTACAGG AGAAGGACAT CCAGATCTGG AAGGTTAGTC TACAGAAGGA ACAAGCCCGG 
TACAGCCAGC TGCAGGATCA GCGAGACACG ATGGTCACCC AGCTCCATAG CCAGATCCGA 
CAGCTGCAGC ATGACCGGGA GGAGTTCTAT AACCAGAGCC AGGAGCTGCA GACGAAGCTG 
GAGGACTGCA GGAACATGAT CGCAGAGCTG CGTCTGGAAT TGAAGAAGGC CAACAGCAAG 
GTGTGTCACA CCGAGCTGCT GCTCAGTCAG GTGTCCCAGA AGCTCTCAAA CAGCGAGTCG 
GTCCAGCAGC AGATGGAGTT CTTGAATCGG CAGCTCTTGG TCCTGGGGGA GGTCAACGAG 
CTGTATCTGG AGCAACTGCA GAGCAAGCAC TCGGATACCA CGAAGGAGGT AGAGATGATG 
AAAGCTGCGT GTCGGAAAGA ATTAGAGAAA AACAGAAGCC ACGTTCTCCA GCAGAATCAG 
AGGCTCGATA CCTCTCAGAA ACGGATTTTG GAACTGGAAT CTCACTTGGC CAAGAAAGAC 
CACCTTCTTT TGGAGCAGAA GAAATATTTA GAGGACGTCA AAGTCCAGGC AAGAGGACAG 
CTGCAGGCGG CAGAGAGCAG GTACGAGGCT CAGAAAAGGA TCACCCAAGT GTTTGAATTG 
GAGATCTTAG ATTTATACGG CAGGTTGGAG AAGGATGGGC TCCTGAAGAA ACTTGAAGAA 
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG CCTGTTGTAA GGATGGCTGC 
TCAGATTCTG TAACAGACCA CCATGAAGAG GCATCTGGCC ACAACGGCGA GACCAAGCCC 
CCCAGGCCTG GTGGTGCACG GGGCAGCAGT GGAAGTAGAG GGGGTGGAGG TGGCAGCAGT 
GGCGGCAGCA GCAGCGAGCT TTCAACCCCA GAGAAACCCC CCAACCAGAG GGCAGGCCTG 
TTCAGCAGTC GGTGGGAAAC GACCGTGGGA GAGCCTGCCA CCAGCATTCC CACTACTGTC 
GGCTCCCTTC CCAGTTCAAA AAGCTTCCTA GGCATGAAGG CACGAGAGTT ATTTCGTAAT 
AAGAGTGAGA GCCAGTGTGA TGAGGACAGT GTGACCATCA GTAGCCTTTC TGAGACGCTA 
AAGACAGAAC TGGGCAAAGA CCTGGGTGTG GAAGCCAAGA CTCCCCCAAC CCTTGATGGC 
CCACACCCCA CCTCCCCGAA CCCAGACAGT GTTGGACAGC TCCACATCAT GGACTACAAT 
GAGACTCATC ATGAACACAG TGAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_014116968.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972443.1 Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

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CloneID	OCe12640
Clone ID Related Accession (Same CDS sequence)	XM_014116967.1 , XM_014116968.1 , XM_005625176.2
Accession Version	XM_014116968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hamartin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACTT CTCCTCCTCC AGCCCCATTG 
TGCCACTCGG ATGACTATGT GCACATTTCC CTCCAGCAGG CCACAGTCCC AGCTCCCAAG 
AAGGAAGAGA AAACAGATTC AGCAAGGCCC TGTCTACACA GACAGCACCA TCTTCCAAAC 
GACAGAGGAT TAGAAGAGCT ACCTGGCCTC AAAGGTTCTG TCACTCTAAG TGATCTTCCA 
GGGTTTTTAG GGGACCTTGC CTCTGATGAA GATAGCGTTG AAAAAGACAA AGAAGAAGCG 
GCGATATCAA AAGAGCTCTC AGAGATCACG ACGGCTGACA CTGAGCCCCT GGTTCCTCGA 
GGAGGCTTTG ACTCTCCCTT CTACCCGGAC AGTCTCCCGG GCTGTCCTCG CAAGACCCAT 
TCAGCCACCT CTGGTCCTCA GGGTACCAGT GTGAATCCTG AACCTTTCAA CTCCTCCTTG 
GACAAGCGTG GGCCAGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGTGGA 
GGCACCCACG AAAGCCCTGC CGGGGACAGG GAGCGCCAGA CTTCGCTGGA GACCAACATC 
CTCACTCCCA GCCCTTGTAA AATTCCACCT CAGAGGGGAG TGGGGTTTGG AAGTGGGCAG 
CCTCCCCCTT ACGATCATCT TTTTGAGGTG GCATTGCCAA AGACCGCCCA TCATTTTGTC 
AGCAAGAAGA CCAAGGAACT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGACTACCCG 
CCCTCAACCT CCCCAGCAGA AGTTCTGGAT AGGCTGATCC AGCAGGGAGC CGACGCGCAC 
AGCAAGGAGC TGAGTCGGTT GTCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA 
GGCTCTCCTC CCTCAGATGA GATCCGAACC CTCCGTAACC AGTTGCTTTT ACTGCACAAC 
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCTC TCCGAAACAG GCGGCTCCTC 
CGCAAGGTGA TCAAGGCGGC AGCTCTGGAG GAGCACAATG CTGCTATGAA AGACCAGTTG 
AAGTTACAGG AGAAGGACAT CCAGATCTGG AAGGTTAGTC TACAGAAGGA ACAAGCCCGG 
TACAGCCAGC TGCAGGATCA GCGAGACACG ATGGTCACCC AGCTCCATAG CCAGATCCGA 
CAGCTGCAGC ATGACCGGGA GGAGTTCTAT AACCAGAGCC AGGAGCTGCA GACGAAGCTG 
GAGGACTGCA GGAACATGAT CGCAGAGCTG CGTCTGGAAT TGAAGAAGGC CAACAGCAAG 
GTGTGTCACA CCGAGCTGCT GCTCAGTCAG GTGTCCCAGA AGCTCTCAAA CAGCGAGTCG 
GTCCAGCAGC AGATGGAGTT CTTGAATCGG CAGCTCTTGG TCCTGGGGGA GGTCAACGAG 
CTGTATCTGG AGCAACTGCA GAGCAAGCAC TCGGATACCA CGAAGGAGGT AGAGATGATG 
AAAGCTGCGT GTCGGAAAGA ATTAGAGAAA AACAGAAGCC ACGTTCTCCA GCAGAATCAG 
AGGCTCGATA CCTCTCAGAA ACGGATTTTG GAACTGGAAT CTCACTTGGC CAAGAAAGAC 
CACCTTCTTT TGGAGCAGAA GAAATATTTA GAGGACGTCA AAGTCCAGGC AAGAGGACAG 
CTGCAGGCGG CAGAGAGCAG GTACGAGGCT CAGAAAAGGA TCACCCAAGT GTTTGAATTG 
GAGATCTTAG ATTTATACGG CAGGTTGGAG AAGGATGGGC TCCTGAAGAA ACTTGAAGAA 
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG CCTGTTGTAA GGATGGCTGC 
TCAGATTCTG TAACAGACCA CCATGAAGAG GCATCTGGCC ACAACGGCGA GACCAAGCCC 
CCCAGGCCTG GTGGTGCACG GGGCAGCAGT GGAAGTAGAG GGGGTGGAGG TGGCAGCAGT 
GGCGGCAGCA GCAGCGAGCT TTCAACCCCA GAGAAACCCC CCAACCAGAG GGCAGGCCTG 
TTCAGCAGTC GGTGGGAAAC GACCGTGGGA GAGCCTGCCA CCAGCATTCC CACTACTGTC 
GGCTCCCTTC CCAGTTCAAA AAGCTTCCTA GGCATGAAGG CACGAGAGTT ATTTCGTAAT 
AAGAGTGAGA GCCAGTGTGA TGAGGACAGT GTGACCATCA GTAGCCTTTC TGAGACGCTA 
AAGACAGAAC TGGGCAAAGA CCTGGGTGTG GAAGCCAAGA CTCCCCCAAC CCTTGATGGC 
CCACACCCCA CCTCCCCGAA CCCAGACAGT GTTGGACAGC TCCACATCAT GGACTACAAT 
GAGACTCATC ATGAACACAG TGAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972443.1
CDS	491..4000
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_014116968.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116968.1

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACTT CTCCTCCTCC AGCCCCATTG 
TGCCACTCGG ATGACTATGT GCACATTTCC CTCCAGCAGG CCACAGTCCC AGCTCCCAAG 
AAGGAAGAGA AAACAGATTC AGCAAGGCCC TGTCTACACA GACAGCACCA TCTTCCAAAC 
GACAGAGGAT TAGAAGAGCT ACCTGGCCTC AAAGGTTCTG TCACTCTAAG TGATCTTCCA 
GGGTTTTTAG GGGACCTTGC CTCTGATGAA GATAGCGTTG AAAAAGACAA AGAAGAAGCG 
GCGATATCAA AAGAGCTCTC AGAGATCACG ACGGCTGACA CTGAGCCCCT GGTTCCTCGA 
GGAGGCTTTG ACTCTCCCTT CTACCCGGAC AGTCTCCCGG GCTGTCCTCG CAAGACCCAT 
TCAGCCACCT CTGGTCCTCA GGGTACCAGT GTGAATCCTG AACCTTTCAA CTCCTCCTTG 
GACAAGCGTG GGCCAGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGTGGA 
GGCACCCACG AAAGCCCTGC CGGGGACAGG GAGCGCCAGA CTTCGCTGGA GACCAACATC 
CTCACTCCCA GCCCTTGTAA AATTCCACCT CAGAGGGGAG TGGGGTTTGG AAGTGGGCAG 
CCTCCCCCTT ACGATCATCT TTTTGAGGTG GCATTGCCAA AGACCGCCCA TCATTTTGTC 
AGCAAGAAGA CCAAGGAACT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGACTACCCG 
CCCTCAACCT CCCCAGCAGA AGTTCTGGAT AGGCTGATCC AGCAGGGAGC CGACGCGCAC 
AGCAAGGAGC TGAGTCGGTT GTCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA 
GGCTCTCCTC CCTCAGATGA GATCCGAACC CTCCGTAACC AGTTGCTTTT ACTGCACAAC 
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCTC TCCGAAACAG GCGGCTCCTC 
CGCAAGGTGA TCAAGGCGGC AGCTCTGGAG GAGCACAATG CTGCTATGAA AGACCAGTTG 
AAGTTACAGG AGAAGGACAT CCAGATCTGG AAGGTTAGTC TACAGAAGGA ACAAGCCCGG 
TACAGCCAGC TGCAGGATCA GCGAGACACG ATGGTCACCC AGCTCCATAG CCAGATCCGA 
CAGCTGCAGC ATGACCGGGA GGAGTTCTAT AACCAGAGCC AGGAGCTGCA GACGAAGCTG 
GAGGACTGCA GGAACATGAT CGCAGAGCTG CGTCTGGAAT TGAAGAAGGC CAACAGCAAG 
GTGTGTCACA CCGAGCTGCT GCTCAGTCAG GTGTCCCAGA AGCTCTCAAA CAGCGAGTCG 
GTCCAGCAGC AGATGGAGTT CTTGAATCGG CAGCTCTTGG TCCTGGGGGA GGTCAACGAG 
CTGTATCTGG AGCAACTGCA GAGCAAGCAC TCGGATACCA CGAAGGAGGT AGAGATGATG 
AAAGCTGCGT GTCGGAAAGA ATTAGAGAAA AACAGAAGCC ACGTTCTCCA GCAGAATCAG 
AGGCTCGATA CCTCTCAGAA ACGGATTTTG GAACTGGAAT CTCACTTGGC CAAGAAAGAC 
CACCTTCTTT TGGAGCAGAA GAAATATTTA GAGGACGTCA AAGTCCAGGC AAGAGGACAG 
CTGCAGGCGG CAGAGAGCAG GTACGAGGCT CAGAAAAGGA TCACCCAAGT GTTTGAATTG 
GAGATCTTAG ATTTATACGG CAGGTTGGAG AAGGATGGGC TCCTGAAGAA ACTTGAAGAA 
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG CCTGTTGTAA GGATGGCTGC 
TCAGATTCTG TAACAGACCA CCATGAAGAG GCATCTGGCC ACAACGGCGA GACCAAGCCC 
CCCAGGCCTG GTGGTGCACG GGGCAGCAGT GGAAGTAGAG GGGGTGGAGG TGGCAGCAGT 
GGCGGCAGCA GCAGCGAGCT TTCAACCCCA GAGAAACCCC CCAACCAGAG GGCAGGCCTG 
TTCAGCAGTC GGTGGGAAAC GACCGTGGGA GAGCCTGCCA CCAGCATTCC CACTACTGTC 
GGCTCCCTTC CCAGTTCAAA AAGCTTCCTA GGCATGAAGG CACGAGAGTT ATTTCGTAAT 
AAGAGTGAGA GCCAGTGTGA TGAGGACAGT GTGACCATCA GTAGCCTTTC TGAGACGCTA 
AAGACAGAAC TGGGCAAAGA CCTGGGTGTG GAAGCCAAGA CTCCCCCAAC CCTTGATGGC 
CCACACCCCA CCTCCCCGAA CCCAGACAGT GTTGGACAGC TCCACATCAT GGACTACAAT 
GAGACTCATC ATGAACACAG TGAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC1 ( XM_005625176.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005625233.1 Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe12640
Clone ID Related Accession (Same CDS sequence)	XM_014116967.1 , XM_014116968.1 , XM_005625176.2
Accession Version	XM_005625176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	hamartin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACTT CTCCTCCTCC AGCCCCATTG 
TGCCACTCGG ATGACTATGT GCACATTTCC CTCCAGCAGG CCACAGTCCC AGCTCCCAAG 
AAGGAAGAGA AAACAGATTC AGCAAGGCCC TGTCTACACA GACAGCACCA TCTTCCAAAC 
GACAGAGGAT TAGAAGAGCT ACCTGGCCTC AAAGGTTCTG TCACTCTAAG TGATCTTCCA 
GGGTTTTTAG GGGACCTTGC CTCTGATGAA GATAGCGTTG AAAAAGACAA AGAAGAAGCG 
GCGATATCAA AAGAGCTCTC AGAGATCACG ACGGCTGACA CTGAGCCCCT GGTTCCTCGA 
GGAGGCTTTG ACTCTCCCTT CTACCCGGAC AGTCTCCCGG GCTGTCCTCG CAAGACCCAT 
TCAGCCACCT CTGGTCCTCA GGGTACCAGT GTGAATCCTG AACCTTTCAA CTCCTCCTTG 
GACAAGCGTG GGCCAGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGTGGA 
GGCACCCACG AAAGCCCTGC CGGGGACAGG GAGCGCCAGA CTTCGCTGGA GACCAACATC 
CTCACTCCCA GCCCTTGTAA AATTCCACCT CAGAGGGGAG TGGGGTTTGG AAGTGGGCAG 
CCTCCCCCTT ACGATCATCT TTTTGAGGTG GCATTGCCAA AGACCGCCCA TCATTTTGTC 
AGCAAGAAGA CCAAGGAACT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGACTACCCG 
CCCTCAACCT CCCCAGCAGA AGTTCTGGAT AGGCTGATCC AGCAGGGAGC CGACGCGCAC 
AGCAAGGAGC TGAGTCGGTT GTCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA 
GGCTCTCCTC CCTCAGATGA GATCCGAACC CTCCGTAACC AGTTGCTTTT ACTGCACAAC 
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCTC TCCGAAACAG GCGGCTCCTC 
CGCAAGGTGA TCAAGGCGGC AGCTCTGGAG GAGCACAATG CTGCTATGAA AGACCAGTTG 
AAGTTACAGG AGAAGGACAT CCAGATCTGG AAGGTTAGTC TACAGAAGGA ACAAGCCCGG 
TACAGCCAGC TGCAGGATCA GCGAGACACG ATGGTCACCC AGCTCCATAG CCAGATCCGA 
CAGCTGCAGC ATGACCGGGA GGAGTTCTAT AACCAGAGCC AGGAGCTGCA GACGAAGCTG 
GAGGACTGCA GGAACATGAT CGCAGAGCTG CGTCTGGAAT TGAAGAAGGC CAACAGCAAG 
GTGTGTCACA CCGAGCTGCT GCTCAGTCAG GTGTCCCAGA AGCTCTCAAA CAGCGAGTCG 
GTCCAGCAGC AGATGGAGTT CTTGAATCGG CAGCTCTTGG TCCTGGGGGA GGTCAACGAG 
CTGTATCTGG AGCAACTGCA GAGCAAGCAC TCGGATACCA CGAAGGAGGT AGAGATGATG 
AAAGCTGCGT GTCGGAAAGA ATTAGAGAAA AACAGAAGCC ACGTTCTCCA GCAGAATCAG 
AGGCTCGATA CCTCTCAGAA ACGGATTTTG GAACTGGAAT CTCACTTGGC CAAGAAAGAC 
CACCTTCTTT TGGAGCAGAA GAAATATTTA GAGGACGTCA AAGTCCAGGC AAGAGGACAG 
CTGCAGGCGG CAGAGAGCAG GTACGAGGCT CAGAAAAGGA TCACCCAAGT GTTTGAATTG 
GAGATCTTAG ATTTATACGG CAGGTTGGAG AAGGATGGGC TCCTGAAGAA ACTTGAAGAA 
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG CCTGTTGTAA GGATGGCTGC 
TCAGATTCTG TAACAGACCA CCATGAAGAG GCATCTGGCC ACAACGGCGA GACCAAGCCC 
CCCAGGCCTG GTGGTGCACG GGGCAGCAGT GGAAGTAGAG GGGGTGGAGG TGGCAGCAGT 
GGCGGCAGCA GCAGCGAGCT TTCAACCCCA GAGAAACCCC CCAACCAGAG GGCAGGCCTG 
TTCAGCAGTC GGTGGGAAAC GACCGTGGGA GAGCCTGCCA CCAGCATTCC CACTACTGTC 
GGCTCCCTTC CCAGTTCAAA AAGCTTCCTA GGCATGAAGG CACGAGAGTT ATTTCGTAAT 
AAGAGTGAGA GCCAGTGTGA TGAGGACAGT GTGACCATCA GTAGCCTTTC TGAGACGCTA 
AAGACAGAAC TGGGCAAAGA CCTGGGTGTG GAAGCCAAGA CTCCCCCAAC CCTTGATGGC 
CCACACCCCA CCTCCCCGAA CCCAGACAGT GTTGGACAGC TCCACATCAT GGACTACAAT 
GAGACTCATC ATGAACACAG TGAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005625233.1
CDS	495..4004
Translation	MAQQANIGELLSMLDSPVLSVRDDVTAVFKENLSSDRGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDKHLLDKMNDCMGRAASRLPALSLLGHVIRLQPPWKHKLSQAPLLPSLLKCLKMDTDVIVLTTGVLVLITMLPMIPQSGKQHLHDFFDIFGRLSSWCLKKPGHVTEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLDTFEEVVRPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGDSISPQVSARFPHRAADVATSPYVDTQNSCGSATSTPYSSSRLTLLNTPGQPPQTLSPPSTRLLTEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTAAGGKGTPLGTPATSPPPAPLCHSDDYVHISLQQATVPAPKKEEKTDSARPCLHRQHHLPNDRGLEELPGLKGSVTLSDLPGFLGDLASDEDSVEKDKEEAAISKELSEITTADTEPLVPRGGFDSPFYPDSLPGCPRKTHSATSGPQGTSVNPEPFNSSLDKRGPDTPKQAFTPIDLPCGGTHESPAGDRERQTSLETNILTPSPCKIPPQRGVGFGSGQPPPYDHLFEVALPKTAHHFVSKKTKELLKKAKGNTEEDYPPSTSPAEVLDRLIQQGADAHSKELSRLSLPSKSVDWTHFGGSPPSDEIRTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQIWKVSLQKEQARYSQLQDQRDTMVTQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRLELKKANSKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQSKHSDTTKEVEMMKAACRKELEKNRSHVLQQNQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKVQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLACCKDGCSDSVTDHHEEASGHNGETKPPRPGGARGSSGSRGGGGGSSGGSSSELSTPEKPPNQRAGLFSSRWETTVGEPATSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTISSLSETLKTELGKDLGVEAKTPPTLDGPHPTSPNPDSVGQLHIMDYNETHHEHSEG

Target ORF information:

RefSeq Version	XM_005625176.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005625176.2

ORF Insert Sequence:

ATGGCCCAGC AAGCGAATAT CGGGGAGCTC CTCTCCATGC TGGACTCCCC TGTGCTGAGT 
GTGCGCGACG ATGTGACAGC TGTCTTCAAA GAGAACCTCA GCTCTGACCG TGGTCCGATG 
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAATT CTCAGCCGGT ATTACACATC 
CTGACTACCT TGCAAGAGCC TCACGACAAG CACCTCTTGG ACAAAATGAA TGACTGTATG 
GGCAGAGCGG CCTCTCGGCT GCCCGCGCTG TCGTTATTGG GGCACGTCAT CAGGCTGCAG 
CCGCCTTGGA AGCACAAACT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT CAAATGTCTC 
AAGATGGACA CCGACGTCAT TGTCCTCACA ACAGGTGTCT TAGTGTTGAT AACCATGCTA 
CCAATGATTC CACAATCGGG GAAGCAGCAC CTTCATGATT TCTTTGATAT TTTTGGCCGT 
CTTTCGTCGT GGTGCCTGAA GAAACCAGGC CATGTGACAG AAATCTATCT TGTCCATCTC 
CATGCCAGTG TTTATGCTCT CTTTCATCGC CTCTACGGAA TGTACCCTTG CAACTTTGTC 
TCCTTTCTAC GCTCTCACTA CAGTATGAAG GAGAACTTGG ACACTTTTGA AGAAGTGGTC 
AGGCCAATGA TGGAGCATGT GCGAATTCAC CCGGAATTAG TGACTGGATC CAAGGACCAC 
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACACATG ATGTTGTAAT AGAGTGTGCC 
AAAATCTCTC TGGACCCCGC AGAAGCCTCA TACGAAGATG GCGACTCCAT ATCCCCGCAG 
GTGTCAGCGC GCTTTCCTCA TCGTGCGGCT GATGTGGCCA CCAGCCCTTA TGTTGACACG 
CAAAACAGCT GTGGGAGTGC TACCTCGACC CCTTATTCCA GCTCTCGGCT GACGTTGCTA 
AATACGCCCG GGCAGCCACC TCAGACTCTG AGTCCCCCGT CGACACGGCT GTTGACTGAG 
CCGCCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT 
TCTCCTGGAA ATGTCCCACC TGATCTCTCA CACCCTTACA GTAAAGTCTT TGGTACAGCT 
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACTT CTCCTCCTCC AGCCCCATTG 
TGCCACTCGG ATGACTATGT GCACATTTCC CTCCAGCAGG CCACAGTCCC AGCTCCCAAG 
AAGGAAGAGA AAACAGATTC AGCAAGGCCC TGTCTACACA GACAGCACCA TCTTCCAAAC 
GACAGAGGAT TAGAAGAGCT ACCTGGCCTC AAAGGTTCTG TCACTCTAAG TGATCTTCCA 
GGGTTTTTAG GGGACCTTGC CTCTGATGAA GATAGCGTTG AAAAAGACAA AGAAGAAGCG 
GCGATATCAA AAGAGCTCTC AGAGATCACG ACGGCTGACA CTGAGCCCCT GGTTCCTCGA 
GGAGGCTTTG ACTCTCCCTT CTACCCGGAC AGTCTCCCGG GCTGTCCTCG CAAGACCCAT 
TCAGCCACCT CTGGTCCTCA GGGTACCAGT GTGAATCCTG AACCTTTCAA CTCCTCCTTG 
GACAAGCGTG GGCCAGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGTGGA 
GGCACCCACG AAAGCCCTGC CGGGGACAGG GAGCGCCAGA CTTCGCTGGA GACCAACATC 
CTCACTCCCA GCCCTTGTAA AATTCCACCT CAGAGGGGAG TGGGGTTTGG AAGTGGGCAG 
CCTCCCCCTT ACGATCATCT TTTTGAGGTG GCATTGCCAA AGACCGCCCA TCATTTTGTC 
AGCAAGAAGA CCAAGGAACT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGACTACCCG 
CCCTCAACCT CCCCAGCAGA AGTTCTGGAT AGGCTGATCC AGCAGGGAGC CGACGCGCAC 
AGCAAGGAGC TGAGTCGGTT GTCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA 
GGCTCTCCTC CCTCAGATGA GATCCGAACC CTCCGTAACC AGTTGCTTTT ACTGCACAAC 
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCTC TCCGAAACAG GCGGCTCCTC 
CGCAAGGTGA TCAAGGCGGC AGCTCTGGAG GAGCACAATG CTGCTATGAA AGACCAGTTG 
AAGTTACAGG AGAAGGACAT CCAGATCTGG AAGGTTAGTC TACAGAAGGA ACAAGCCCGG 
TACAGCCAGC TGCAGGATCA GCGAGACACG ATGGTCACCC AGCTCCATAG CCAGATCCGA 
CAGCTGCAGC ATGACCGGGA GGAGTTCTAT AACCAGAGCC AGGAGCTGCA GACGAAGCTG 
GAGGACTGCA GGAACATGAT CGCAGAGCTG CGTCTGGAAT TGAAGAAGGC CAACAGCAAG 
GTGTGTCACA CCGAGCTGCT GCTCAGTCAG GTGTCCCAGA AGCTCTCAAA CAGCGAGTCG 
GTCCAGCAGC AGATGGAGTT CTTGAATCGG CAGCTCTTGG TCCTGGGGGA GGTCAACGAG 
CTGTATCTGG AGCAACTGCA GAGCAAGCAC TCGGATACCA CGAAGGAGGT AGAGATGATG 
AAAGCTGCGT GTCGGAAAGA ATTAGAGAAA AACAGAAGCC ACGTTCTCCA GCAGAATCAG 
AGGCTCGATA CCTCTCAGAA ACGGATTTTG GAACTGGAAT CTCACTTGGC CAAGAAAGAC 
CACCTTCTTT TGGAGCAGAA GAAATATTTA GAGGACGTCA AAGTCCAGGC AAGAGGACAG 
CTGCAGGCGG CAGAGAGCAG GTACGAGGCT CAGAAAAGGA TCACCCAAGT GTTTGAATTG 
GAGATCTTAG ATTTATACGG CAGGTTGGAG AAGGATGGGC TCCTGAAGAA ACTTGAAGAA 
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG CCTGTTGTAA GGATGGCTGC 
TCAGATTCTG TAACAGACCA CCATGAAGAG GCATCTGGCC ACAACGGCGA GACCAAGCCC 
CCCAGGCCTG GTGGTGCACG GGGCAGCAGT GGAAGTAGAG GGGGTGGAGG TGGCAGCAGT 
GGCGGCAGCA GCAGCGAGCT TTCAACCCCA GAGAAACCCC CCAACCAGAG GGCAGGCCTG 
TTCAGCAGTC GGTGGGAAAC GACCGTGGGA GAGCCTGCCA CCAGCATTCC CACTACTGTC 
GGCTCCCTTC CCAGTTCAAA AAGCTTCCTA GGCATGAAGG CACGAGAGTT ATTTCGTAAT 
AAGAGTGAGA GCCAGTGTGA TGAGGACAGT GTGACCATCA GTAGCCTTTC TGAGACGCTA 
AAGACAGAAC TGGGCAAAGA CCTGGGTGTG GAAGCCAAGA CTCCCCCAAC CCTTGATGGC 
CCACACCCCA CCTCCCCGAA CCCAGACAGT GTTGGACAGC TCCACATCAT GGACTACAAT 
GAGACTCATC ATGAACACAG TGAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TSC1
Entrez Gene ID	480688
Full Name	TSC complex subunit 1
General protein information	Preferred Names TSC complex subunit 1 Names hamartin chromosome 9 TSC1 tuberous sclerosis 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	TSC1	NP_000359.1
Bos taurus (cattle)	TSC1	XP_005213508.1
	TSC1	XP_001103846.2
Mus musculus (house mouse)	Tsc1	NP_075025.2
Xenopus tropicalis (tropical clawed frog)	tsc1	XP_002944681.2
Pan troglodytes (chimpanzee)	TSC1	XP_001168992.1
Gallus gallus (chicken)	TSC1	XP_415449.2
Canis lupus familiaris (dog)	TSC1	XP_005625233.1
Rattus norvegicus (Norway rat)	Tsc1	NP_068626.1
Danio rerio (zebrafish)	tsc1a	NP_956346.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TSC1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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