UBE4A cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE4A
Entrez Gene ID	479418
Full Name	ubiquitination factor E4A
General protein information	Preferred Names ubiquitination factor E4A Names ubiquitin conjugation factor E4 A ubiquitination factor E4A (UFD2 homolog, yeast)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_844988.4	XP_850081.1	ubiquitin conjugation factor E4 A
XM_844988.5	XP_850081.1	ubiquitin conjugation factor E4 A

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE4A	NP_004779.2
Bos taurus (cattle)	UBE4A	NP_001092469.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001681	XP_321414.5
Pan troglodytes (chimpanzee)	UBE4A	XP_508791.2
Xenopus tropicalis (tropical clawed frog)	ube4a	NP_001163971.1
Canis lupus familiaris (dog)	UBE4A	XP_850081.1
Mus musculus (house mouse)	Ube4a	NP_663375.3
Danio rerio (zebrafish)	ube4a	XP_001922961.2
Drosophila melanogaster (fruit fly)	CG11070	NP_609060.1
	UBE4A	XP_001097911.2
Rattus norvegicus (Norway rat)	Ube4a	NP_997493.1
Gallus gallus (chicken)	UBE4A	NP_001026081.1

Gene Ontology

The following UBE4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe05907	XM_844988.5 Latest version!	Canis lupus familiaris ubiquitination factor E4A (UBE4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe05907	XM_844988.4	Canis lupus familiaris ubiquitination factor E4A (UBE4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE4A ( XM_844988.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_850081.1 Canis lupus familiaris ubiquitination factor E4A (UBE4A), mRNA.

Price & Availability↑

CloneID	OCe05907
Clone ID Related Accession (Same CDS sequence)	XM_844988.4 , XM_844988.5
Accession Version	XM_844988.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ubiquitin conjugation factor E4 A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_844988.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACC AGGAGAATAA CAACAACATC TCGAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA GTTTGCAGCA ATCCAAAAAG AGCAGCTGAA GCAGCAATCT 
GATGAACTCA CAGCTAGCCC AGATGACTCG GATAATAGTG TGTCAGAAAG CCTGGATGAA 
TTTGATTACT CTGTGGCTGA GATTAGCCGT TCCTTCCGTT CACAGCAAGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTCA TTACTCTGGA CAACAGTGAC 
CCAAGCTTGA AAAGTGGGAA TGGCATCCCC AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGACCTGG AAGATCAAGA CTGGCTTGAT ATGAACAACG TTGAGCAGGC TGTCTTCGCT 
CGCTTATTAC TTCAGGATCC AGGCAACCAC TTGATTAACA TGACTTCTTC TACAACGTTG 
AATCTCTCTG CTGATCGAGA TGCAGGGGAG AGGCACATTT TTTGTTACCT TTATTCCTGC 
TTCCAAAGAG CTAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTCCC CTTTGCGGTG 
CAGTGCAGGA ACCTCACTGT GTCCAATACC CGAACAGTAC TCCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTAATGT TGGAAGCCAT CCAAGGAGCC 
CATTTTGAAG ATGTAACTGA GTTTCTGGAA GAGGTTATTG AAGCCTTGAT AATGGATGAG 
GAAGTTCGCA CATTTCCCGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG AACTATGTCA GATCCTGTTT TATGCATATC TGGATATTCT TCTCTATTTC 
ACTAGGCAGA AGGATATGGC AAAGGTTTTT GTAGAGTACA TTCAGCCCAA GGACCCTAGC 
AATGGGCAGA TGTACCAGAA GACCTTGCTG GGAGTAATCC TGAATATCTC CTGCTTATTA 
AAGACTCCGG GTGTAGTAGA AAATCATGGC TACTTTCTGA ATCCATCTCG TTCCAGTCCC 
CAGGAGATCA AAGTGCAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCATGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTCTTCCA AATGTATGCC TCGGATGCCT TCTTTTTGAA TCTGGGTGCT 
GCTCTCCTGA AGTTATGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACTT ACTGTGCCCT GAAGGAATTG AATGATGAAG AACGAAAAAT TAAAAATGTG 
CACATGAGAG GTTTGGACAA AGAAACTTGT TTGATCCCAG CGGTGCAAGA GCCAAAGTTT 
CCCCAGAACT ACAACCTTGT GACAGAGAAC CTTGTCCTGA CAGAGTATAC TTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAACCT TCGTGAGCAA 
TTTGAACGGC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACAGA GCCACAGATG 
CTACAGAACT GTCTAAACTT GCAGGTGTCC ATGGCTGTTC TGCTTGTTCA ACTGGCCATA 
GGCAATGAGG GCTCACAGCC AATAGAACTA ACTTTTCCTT TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACTTGGGTG ATTTCCTCAT TTTTCTACGC 
CGCTTTGCCG ATGACATCTT GGAGACGTCA GCAGACTCCC TGGAACATGT CCTTCACTTT 
ATCACGATTT TCACTGGAAG CATAGAAAGG ATGAAGAATC CCCACCTACG GGCCAAACTG 
GCGGAGGTGC TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTCCTC ATGCACCCCA CCTTGCAGAA 
GCTCTAATCA AGGTTTTTGT GGACATTGAG TTTACAGGAG ACCCCCATCA ATTTGAACAG 
AAGTTTAATT ACCGGCGTCC CATGTATCCC ATCCTAAGGT ACATGTGGGG AACAGATACC 
TATCGAGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGTTT TCTTAACCTG CTAATGAACG ACGCCATCTT CCTCCTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAACAGA TTGAGAAAGA CCGAGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAGAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TTGGTACCCT TGCCTTTCTG 
ACATCAGAGA TCAAATCACT CTTTGTGCAT CCTTTCCTGG CTGAGCGCAT CATCTCCATG 
TTGAACTACT TCCTGCAGCA CCTGGTTGGC CCCAAGATGG GGGCCTTAAA AGTCAAGGAC 
TTCAGTGAAT TTGACTTCAA GCCCCAGCAG CTCGTATCAG ATATCTGCAC AATCTACTTA 
AACCTTGGGG ACGAGGAGAA CTTCTGTGCC ACGGTGCCCA AGGACGGACG TTCCTATTCC 
CCAACTCTCT TTGCACAGAC AGTCCGAGTC CTGAAGAAAA TAAATAAGCC CGGGAATATG 
ATTGTGGCTT TCAGCAACTT GGCAGAGAGA ATCAAGTCTC TAGCAGACCT CCAGCAACAG 
GAGGAGGAAA CTTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATCAT GAGCACACTC 
ATGTCCGACC CTGTGGTGCT GCCATCCTCC AGAGTCACTG TGGATAGATC CACCATTGCC 
AGACATTTGC TCAGTGACCA AACCGACCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGAT GGCTTGCAGA GAGGAAACAA 
CAAAAGGAGC AACTTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_850081.1
CDS	143..3343
Translation	MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELTASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVDLEDQDWLDMNNVEQAVFARLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALIMDEEVRTFPEVMIPVFDILLGRIKDLELCQILFYAYLDILLYFTRQKDMAKVFVEYIQPKDPSNGQMYQKTLLGVILNISCLLKTPGVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKFPQNYNLVTENLVLTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFPHAPHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMSDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Target ORF information:

RefSeq Version	XM_844988.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitination factor E4A (UBE4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_844988.4

ORF Insert Sequence:

ATGACAGACC AGGAGAATAA CAACAACATC TCGAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA GTTTGCAGCA ATCCAAAAAG AGCAGCTGAA GCAGCAATCT 
GATGAACTCA CAGCTAGCCC AGATGACTCG GATAATAGTG TGTCAGAAAG CCTGGATGAA 
TTTGATTACT CTGTGGCTGA GATTAGCCGT TCCTTCCGTT CACAGCAAGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTCA TTACTCTGGA CAACAGTGAC 
CCAAGCTTGA AAAGTGGGAA TGGCATCCCC AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGACCTGG AAGATCAAGA CTGGCTTGAT ATGAACAACG TTGAGCAGGC TGTCTTCGCT 
CGCTTATTAC TTCAGGATCC AGGCAACCAC TTGATTAACA TGACTTCTTC TACAACGTTG 
AATCTCTCTG CTGATCGAGA TGCAGGGGAG AGGCACATTT TTTGTTACCT TTATTCCTGC 
TTCCAAAGAG CTAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTCCC CTTTGCGGTG 
CAGTGCAGGA ACCTCACTGT GTCCAATACC CGAACAGTAC TCCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTAATGT TGGAAGCCAT CCAAGGAGCC 
CATTTTGAAG ATGTAACTGA GTTTCTGGAA GAGGTTATTG AAGCCTTGAT AATGGATGAG 
GAAGTTCGCA CATTTCCCGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG AACTATGTCA GATCCTGTTT TATGCATATC TGGATATTCT TCTCTATTTC 
ACTAGGCAGA AGGATATGGC AAAGGTTTTT GTAGAGTACA TTCAGCCCAA GGACCCTAGC 
AATGGGCAGA TGTACCAGAA GACCTTGCTG GGAGTAATCC TGAATATCTC CTGCTTATTA 
AAGACTCCGG GTGTAGTAGA AAATCATGGC TACTTTCTGA ATCCATCTCG TTCCAGTCCC 
CAGGAGATCA AAGTGCAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCATGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTCTTCCA AATGTATGCC TCGGATGCCT TCTTTTTGAA TCTGGGTGCT 
GCTCTCCTGA AGTTATGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACTT ACTGTGCCCT GAAGGAATTG AATGATGAAG AACGAAAAAT TAAAAATGTG 
CACATGAGAG GTTTGGACAA AGAAACTTGT TTGATCCCAG CGGTGCAAGA GCCAAAGTTT 
CCCCAGAACT ACAACCTTGT GACAGAGAAC CTTGTCCTGA CAGAGTATAC TTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAACCT TCGTGAGCAA 
TTTGAACGGC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACAGA GCCACAGATG 
CTACAGAACT GTCTAAACTT GCAGGTGTCC ATGGCTGTTC TGCTTGTTCA ACTGGCCATA 
GGCAATGAGG GCTCACAGCC AATAGAACTA ACTTTTCCTT TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACTTGGGTG ATTTCCTCAT TTTTCTACGC 
CGCTTTGCCG ATGACATCTT GGAGACGTCA GCAGACTCCC TGGAACATGT CCTTCACTTT 
ATCACGATTT TCACTGGAAG CATAGAAAGG ATGAAGAATC CCCACCTACG GGCCAAACTG 
GCGGAGGTGC TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTCCTC ATGCACCCCA CCTTGCAGAA 
GCTCTAATCA AGGTTTTTGT GGACATTGAG TTTACAGGAG ACCCCCATCA ATTTGAACAG 
AAGTTTAATT ACCGGCGTCC CATGTATCCC ATCCTAAGGT ACATGTGGGG AACAGATACC 
TATCGAGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGTTT TCTTAACCTG CTAATGAACG ACGCCATCTT CCTCCTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAACAGA TTGAGAAAGA CCGAGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAGAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TTGGTACCCT TGCCTTTCTG 
ACATCAGAGA TCAAATCACT CTTTGTGCAT CCTTTCCTGG CTGAGCGCAT CATCTCCATG 
TTGAACTACT TCCTGCAGCA CCTGGTTGGC CCCAAGATGG GGGCCTTAAA AGTCAAGGAC 
TTCAGTGAAT TTGACTTCAA GCCCCAGCAG CTCGTATCAG ATATCTGCAC AATCTACTTA 
AACCTTGGGG ACGAGGAGAA CTTCTGTGCC ACGGTGCCCA AGGACGGACG TTCCTATTCC 
CCAACTCTCT TTGCACAGAC AGTCCGAGTC CTGAAGAAAA TAAATAAGCC CGGGAATATG 
ATTGTGGCTT TCAGCAACTT GGCAGAGAGA ATCAAGTCTC TAGCAGACCT CCAGCAACAG 
GAGGAGGAAA CTTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATCAT GAGCACACTC 
ATGTCCGACC CTGTGGTGCT GCCATCCTCC AGAGTCACTG TGGATAGATC CACCATTGCC 
AGACATTTGC TCAGTGACCA AACCGACCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGAT GGCTTGCAGA GAGGAAACAA 
CAAAAGGAGC AACTTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4A ( XM_844988.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_850081.1 Canis lupus familiaris ubiquitination factor E4A (UBE4A), mRNA.

Price & Availability↑

CloneID	OCe05907
Clone ID Related Accession (Same CDS sequence)	XM_844988.4 , XM_844988.5
Accession Version	XM_844988.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin conjugation factor E4 A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_844988.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACC AGGAGAATAA CAACAACATC TCGAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA GTTTGCAGCA ATCCAAAAAG AGCAGCTGAA GCAGCAATCT 
GATGAACTCA CAGCTAGCCC AGATGACTCG GATAATAGTG TGTCAGAAAG CCTGGATGAA 
TTTGATTACT CTGTGGCTGA GATTAGCCGT TCCTTCCGTT CACAGCAAGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTCA TTACTCTGGA CAACAGTGAC 
CCAAGCTTGA AAAGTGGGAA TGGCATCCCC AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGACCTGG AAGATCAAGA CTGGCTTGAT ATGAACAACG TTGAGCAGGC TGTCTTCGCT 
CGCTTATTAC TTCAGGATCC AGGCAACCAC TTGATTAACA TGACTTCTTC TACAACGTTG 
AATCTCTCTG CTGATCGAGA TGCAGGGGAG AGGCACATTT TTTGTTACCT TTATTCCTGC 
TTCCAAAGAG CTAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTCCC CTTTGCGGTG 
CAGTGCAGGA ACCTCACTGT GTCCAATACC CGAACAGTAC TCCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTAATGT TGGAAGCCAT CCAAGGAGCC 
CATTTTGAAG ATGTAACTGA GTTTCTGGAA GAGGTTATTG AAGCCTTGAT AATGGATGAG 
GAAGTTCGCA CATTTCCCGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG AACTATGTCA GATCCTGTTT TATGCATATC TGGATATTCT TCTCTATTTC 
ACTAGGCAGA AGGATATGGC AAAGGTTTTT GTAGAGTACA TTCAGCCCAA GGACCCTAGC 
AATGGGCAGA TGTACCAGAA GACCTTGCTG GGAGTAATCC TGAATATCTC CTGCTTATTA 
AAGACTCCGG GTGTAGTAGA AAATCATGGC TACTTTCTGA ATCCATCTCG TTCCAGTCCC 
CAGGAGATCA AAGTGCAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCATGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTCTTCCA AATGTATGCC TCGGATGCCT TCTTTTTGAA TCTGGGTGCT 
GCTCTCCTGA AGTTATGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACTT ACTGTGCCCT GAAGGAATTG AATGATGAAG AACGAAAAAT TAAAAATGTG 
CACATGAGAG GTTTGGACAA AGAAACTTGT TTGATCCCAG CGGTGCAAGA GCCAAAGTTT 
CCCCAGAACT ACAACCTTGT GACAGAGAAC CTTGTCCTGA CAGAGTATAC TTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAACCT TCGTGAGCAA 
TTTGAACGGC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACAGA GCCACAGATG 
CTACAGAACT GTCTAAACTT GCAGGTGTCC ATGGCTGTTC TGCTTGTTCA ACTGGCCATA 
GGCAATGAGG GCTCACAGCC AATAGAACTA ACTTTTCCTT TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACTTGGGTG ATTTCCTCAT TTTTCTACGC 
CGCTTTGCCG ATGACATCTT GGAGACGTCA GCAGACTCCC TGGAACATGT CCTTCACTTT 
ATCACGATTT TCACTGGAAG CATAGAAAGG ATGAAGAATC CCCACCTACG GGCCAAACTG 
GCGGAGGTGC TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTCCTC ATGCACCCCA CCTTGCAGAA 
GCTCTAATCA AGGTTTTTGT GGACATTGAG TTTACAGGAG ACCCCCATCA ATTTGAACAG 
AAGTTTAATT ACCGGCGTCC CATGTATCCC ATCCTAAGGT ACATGTGGGG AACAGATACC 
TATCGAGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGTTT TCTTAACCTG CTAATGAACG ACGCCATCTT CCTCCTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAACAGA TTGAGAAAGA CCGAGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAGAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TTGGTACCCT TGCCTTTCTG 
ACATCAGAGA TCAAATCACT CTTTGTGCAT CCTTTCCTGG CTGAGCGCAT CATCTCCATG 
TTGAACTACT TCCTGCAGCA CCTGGTTGGC CCCAAGATGG GGGCCTTAAA AGTCAAGGAC 
TTCAGTGAAT TTGACTTCAA GCCCCAGCAG CTCGTATCAG ATATCTGCAC AATCTACTTA 
AACCTTGGGG ACGAGGAGAA CTTCTGTGCC ACGGTGCCCA AGGACGGACG TTCCTATTCC 
CCAACTCTCT TTGCACAGAC AGTCCGAGTC CTGAAGAAAA TAAATAAGCC CGGGAATATG 
ATTGTGGCTT TCAGCAACTT GGCAGAGAGA ATCAAGTCTC TAGCAGACCT CCAGCAACAG 
GAGGAGGAAA CTTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATCAT GAGCACACTC 
ATGTCCGACC CTGTGGTGCT GCCATCCTCC AGAGTCACTG TGGATAGATC CACCATTGCC 
AGACATTTGC TCAGTGACCA AACCGACCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGAT GGCTTGCAGA GAGGAAACAA 
CAAAAGGAGC AACTTGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_850081.1
CDS	180..3380
Translation	MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELTASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVDLEDQDWLDMNNVEQAVFARLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALIMDEEVRTFPEVMIPVFDILLGRIKDLELCQILFYAYLDILLYFTRQKDMAKVFVEYIQPKDPSNGQMYQKTLLGVILNISCLLKTPGVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKFPQNYNLVTENLVLTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFPHAPHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMSDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Target ORF information:

RefSeq Version	XM_844988.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitination factor E4A (UBE4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_844988.5

ORF Insert Sequence:

ATGACAGACC AGGAGAATAA CAACAACATC TCGAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAGCA GTTTGCAGCA ATCCAAAAAG AGCAGCTGAA GCAGCAATCT 
GATGAACTCA CAGCTAGCCC AGATGACTCG GATAATAGTG TGTCAGAAAG CCTGGATGAA 
TTTGATTACT CTGTGGCTGA GATTAGCCGT TCCTTCCGTT CACAGCAAGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTCA TTACTCTGGA CAACAGTGAC 
CCAAGCTTGA AAAGTGGGAA TGGCATCCCC AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGACCTGG AAGATCAAGA CTGGCTTGAT ATGAACAACG TTGAGCAGGC TGTCTTCGCT 
CGCTTATTAC TTCAGGATCC AGGCAACCAC TTGATTAACA TGACTTCTTC TACAACGTTG 
AATCTCTCTG CTGATCGAGA TGCAGGGGAG AGGCACATTT TTTGTTACCT TTATTCCTGC 
TTCCAAAGAG CTAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTCCC CTTTGCGGTG 
CAGTGCAGGA ACCTCACTGT GTCCAATACC CGAACAGTAC TCCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTAATGT TGGAAGCCAT CCAAGGAGCC 
CATTTTGAAG ATGTAACTGA GTTTCTGGAA GAGGTTATTG AAGCCTTGAT AATGGATGAG 
GAAGTTCGCA CATTTCCCGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG AACTATGTCA GATCCTGTTT TATGCATATC TGGATATTCT TCTCTATTTC 
ACTAGGCAGA AGGATATGGC AAAGGTTTTT GTAGAGTACA TTCAGCCCAA GGACCCTAGC 
AATGGGCAGA TGTACCAGAA GACCTTGCTG GGAGTAATCC TGAATATCTC CTGCTTATTA 
AAGACTCCGG GTGTAGTAGA AAATCATGGC TACTTTCTGA ATCCATCTCG TTCCAGTCCC 
CAGGAGATCA AAGTGCAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCATGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTCTTCCA AATGTATGCC TCGGATGCCT TCTTTTTGAA TCTGGGTGCT 
GCTCTCCTGA AGTTATGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACTT ACTGTGCCCT GAAGGAATTG AATGATGAAG AACGAAAAAT TAAAAATGTG 
CACATGAGAG GTTTGGACAA AGAAACTTGT TTGATCCCAG CGGTGCAAGA GCCAAAGTTT 
CCCCAGAACT ACAACCTTGT GACAGAGAAC CTTGTCCTGA CAGAGTATAC TTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAACCT TCGTGAGCAA 
TTTGAACGGC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACAGA GCCACAGATG 
CTACAGAACT GTCTAAACTT GCAGGTGTCC ATGGCTGTTC TGCTTGTTCA ACTGGCCATA 
GGCAATGAGG GCTCACAGCC AATAGAACTA ACTTTTCCTT TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACTTGGGTG ATTTCCTCAT TTTTCTACGC 
CGCTTTGCCG ATGACATCTT GGAGACGTCA GCAGACTCCC TGGAACATGT CCTTCACTTT 
ATCACGATTT TCACTGGAAG CATAGAAAGG ATGAAGAATC CCCACCTACG GGCCAAACTG 
GCGGAGGTGC TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTCCTC ATGCACCCCA CCTTGCAGAA 
GCTCTAATCA AGGTTTTTGT GGACATTGAG TTTACAGGAG ACCCCCATCA ATTTGAACAG 
AAGTTTAATT ACCGGCGTCC CATGTATCCC ATCCTAAGGT ACATGTGGGG AACAGATACC 
TATCGAGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGTTT TCTTAACCTG CTAATGAACG ACGCCATCTT CCTCCTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAACAGA TTGAGAAAGA CCGAGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAGAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TTGGTACCCT TGCCTTTCTG 
ACATCAGAGA TCAAATCACT CTTTGTGCAT CCTTTCCTGG CTGAGCGCAT CATCTCCATG 
TTGAACTACT TCCTGCAGCA CCTGGTTGGC CCCAAGATGG GGGCCTTAAA AGTCAAGGAC 
TTCAGTGAAT TTGACTTCAA GCCCCAGCAG CTCGTATCAG ATATCTGCAC AATCTACTTA 
AACCTTGGGG ACGAGGAGAA CTTCTGTGCC ACGGTGCCCA AGGACGGACG TTCCTATTCC 
CCAACTCTCT TTGCACAGAC AGTCCGAGTC CTGAAGAAAA TAAATAAGCC CGGGAATATG 
ATTGTGGCTT TCAGCAACTT GGCAGAGAGA ATCAAGTCTC TAGCAGACCT CCAGCAACAG 
GAGGAGGAAA CTTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATCAT GAGCACACTC 
ATGTCCGACC CTGTGGTGCT GCCATCCTCC AGAGTCACTG TGGATAGATC CACCATTGCC 
AGACATTTGC TCAGTGACCA AACCGACCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGAT GGCTTGCAGA GAGGAAACAA 
CAAAAGGAGC AACTTGAATA A