POLR1E cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR1E
Entrez Gene ID	474767
Full Name	RNA polymerase I subunit E
General protein information	Preferred Names RNA polymerase I subunit E Names DNA-directed RNA polymerase I subunit RPA49 polymerase (RNA) I polypeptide E, 53kDa
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_531998.5	XP_531998.2	DNA-directed RNA polymerase I subunit RPA49
XM_531998.4	XP_531998.2	DNA-directed RNA polymerase I subunit RPA49

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Polr1e	NP_001101408.1
Xenopus tropicalis (tropical clawed frog)	polr1e	NP_001120306.1
Gallus gallus (chicken)	POLR1E	XP_424416.3
Danio rerio (zebrafish)	polr1e	NP_001018540.1
Mus musculus (house mouse)	Polr1e	NP_073722.1
Homo sapiens (human)	POLR1E	NP_071935.1
Pan troglodytes (chimpanzee)	POLR1E	XP_001169824.1
Canis lupus familiaris (dog)	POLR1E	XP_531998.2
Bos taurus (cattle)	POLR1E	NP_001029580.1

Gene Ontology

The following POLR1E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR1E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe14643	XM_531998.5 Latest version!	Canis lupus familiaris RNA polymerase I subunit E (POLR1E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
OCe14643	XM_531998.4	Canis lupus familiaris polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR1E ( XM_531998.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_531998.2 Canis lupus familiaris RNA polymerase I subunit E (POLR1E), mRNA.

Price & Availability↑

CloneID	OCe14643
Clone ID Related Accession (Same CDS sequence)	XM_531998.5 , XM_531998.4
Accession Version	XM_531998.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA-directed RNA polymerase I subunit RPA49
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_531998.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGGTGTTGCC GAGTGCGCGG TGGCTATACT GTGGGCAGCC CGACGAGAGC 
CAGAGAGCCG TACTGGTGCA GTTCTCCAAC GGGAAGCTGC AGAATCCAGG CAACGTGCGT 
TTTACTTTGT ACAAGAGCAA TGACTCCACA AACCCCAGGA AGAGGAGTCA GCGGATTCTG 
GCAGCTGAAA CAGACAGACT TTCCTACGTG GGAAACAATT TCGGGACTGG AGCCCTCAAA 
TGCAACACTT TGTGCAGGCA CTTTGTGGGA ATTCTCAACA AGACCTCTGG CCAGATGCAA 
GTGTATGATG CTGAATTGTT CAACATGCAG CCACTGTTTG CAGATGAATC ACTTGAGAGC 
GACTTGACAC AAGAGAGTCA GGCCAAAACT TTTAGAGAAA AGATGGATTC TTGCATTGAA 
GCCTTTGGAA CCACCAAACA GAAGCGGGCT TTGAACTCCA GAAGAATGAA CACAGTTGGC 
AGTGAATCTC TGAACTGTGC AGTAGCTAAA GCTGCAGAGA ATATTATTGA TGCGAAGGGT 
GTAACTGCTC TGGTCAACGA TGCCATCCAT GATGATCTGC AGGATGACTC CCTCTACCTT 
CCTCCCTGCC ACGCTGATGC AGCCAAGCCT GAAGACGTAT ATAAATTTGA AGACCTTCTG 
TCCACTGCGG AGTATGAAGC TCTTCAGAGC CCATCTGAAG CTTTCAGGAA TGTCTCATCA 
GAGGAAATAC TGAAGATGAT TGAAGAGAAC AGCCACTGCT CCTTTGTCAT AGAAGCATTG 
AAGTCTTTGC CATCAGATGA GAAGAGCCGA GACCGCCAGG CCCGATGCAT ATGGTTTCTG 
GACACGCTCA TCAAATTTCG AGCACAGAAA GTGGTTAAGC GGAAAAGTGC CCTGGGACCC 
GGAATCCCCC ATGTCATCAA CACCAAATTG CTGAAGCACT TCACCTGCTT GACCTACAAC 
AACGGCAGCC TACGGAACTT AATTTCGGAT TCTATGAAGG CGAAGATCAC TGCATATGTA 
ATCATACTTG CCTTGCACAT AAGCGACTTC CAGATTGACC TGACAATGTT ACAGAGGGAC 
TTGAAGCTTA GTGAGAGAAG GATGATTGAG ATAGCGAAAG CCATGAGGCT GAAGATCTCT 
AAAAGAAAGG TGTCTCTGGC AGCTGGCAGG GAAGAGGATC ACAAACTGGG CACCCTGTCC 
ATCCCGCTGC CTCCAGCCCA GACCTCAAAA CACCAGTCAA AGCGGAGGAA AATCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_531998.2
CDS	117..1376
Translation	MAAEVLPSARWLYCGQPDESQRAVLVQFSNGKLQNPGNVRFTLYKSNDSTNPRKRSQRILAAETDRLSYVGNNFGTGALKCNTLCRHFVGILNKTSGQMQVYDAELFNMQPLFADESLESDLTQESQAKTFREKMDSCIEAFGTTKQKRALNSRRMNTVGSESLNCAVAKAAENIIDAKGVTALVNDAIHDDLQDDSLYLPPCHADAAKPEDVYKFEDLLSTAEYEALQSPSEAFRNVSSEEILKMIEENSHCSFVIEALKSLPSDEKSRDRQARCIWFLDTLIKFRAQKVVKRKSALGPGIPHVINTKLLKHFTCLTYNNGSLRNLISDSMKAKITAYVIILALHISDFQIDLTMLQRDLKLSERRMIEIAKAMRLKISKRKVSLAAGREEDHKLGTLSIPLPPAQTSKHQSKRRKIT

Target ORF information:

RefSeq Version	XM_531998.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RNA polymerase I subunit E (POLR1E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_531998.5

ORF Insert Sequence:

ATGGCGGCGG AGGTGTTGCC GAGTGCGCGG TGGCTATACT GTGGGCAGCC CGACGAGAGC 
CAGAGAGCCG TACTGGTGCA GTTCTCCAAC GGGAAGCTGC AGAATCCAGG CAACGTGCGT 
TTTACTTTGT ACAAGAGCAA TGACTCCACA AACCCCAGGA AGAGGAGTCA GCGGATTCTG 
GCAGCTGAAA CAGACAGACT TTCCTACGTG GGAAACAATT TCGGGACTGG AGCCCTCAAA 
TGCAACACTT TGTGCAGGCA CTTTGTGGGA ATTCTCAACA AGACCTCTGG CCAGATGCAA 
GTGTATGATG CTGAATTGTT CAACATGCAG CCACTGTTTG CAGATGAATC ACTTGAGAGC 
GACTTGACAC AAGAGAGTCA GGCCAAAACT TTTAGAGAAA AGATGGATTC TTGCATTGAA 
GCCTTTGGAA CCACCAAACA GAAGCGGGCT TTGAACTCCA GAAGAATGAA CACAGTTGGC 
AGTGAATCTC TGAACTGTGC AGTAGCTAAA GCTGCAGAGA ATATTATTGA TGCGAAGGGT 
GTAACTGCTC TGGTCAACGA TGCCATCCAT GATGATCTGC AGGATGACTC CCTCTACCTT 
CCTCCCTGCC ACGCTGATGC AGCCAAGCCT GAAGACGTAT ATAAATTTGA AGACCTTCTG 
TCCACTGCGG AGTATGAAGC TCTTCAGAGC CCATCTGAAG CTTTCAGGAA TGTCTCATCA 
GAGGAAATAC TGAAGATGAT TGAAGAGAAC AGCCACTGCT CCTTTGTCAT AGAAGCATTG 
AAGTCTTTGC CATCAGATGA GAAGAGCCGA GACCGCCAGG CCCGATGCAT ATGGTTTCTG 
GACACGCTCA TCAAATTTCG AGCACAGAAA GTGGTTAAGC GGAAAAGTGC CCTGGGACCC 
GGAATCCCCC ATGTCATCAA CACCAAATTG CTGAAGCACT TCACCTGCTT GACCTACAAC 
AACGGCAGCC TACGGAACTT AATTTCGGAT TCTATGAAGG CGAAGATCAC TGCATATGTA 
ATCATACTTG CCTTGCACAT AAGCGACTTC CAGATTGACC TGACAATGTT ACAGAGGGAC 
TTGAAGCTTA GTGAGAGAAG GATGATTGAG ATAGCGAAAG CCATGAGGCT GAAGATCTCT 
AAAAGAAAGG TGTCTCTGGC AGCTGGCAGG GAAGAGGATC ACAAACTGGG CACCCTGTCC 
ATCCCGCTGC CTCCAGCCCA GACCTCAAAA CACCAGTCAA AGCGGAGGAA AATCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR1E ( XM_531998.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_531998.2 Canis lupus familiaris polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mRNA.

Price & Availability↑

CloneID	OCe14643
Clone ID Related Accession (Same CDS sequence)	XM_531998.5 , XM_531998.4
Accession Version	XM_531998.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA-directed RNA polymerase I subunit RPA49
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_531998.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGGTGTTGCC GAGTGCGCGG TGGCTATACT GTGGGCAGCC CGACGAGAGC 
CAGAGAGCCG TACTGGTGCA GTTCTCCAAC GGGAAGCTGC AGAATCCAGG CAACGTGCGT 
TTTACTTTGT ACAAGAGCAA TGACTCCACA AACCCCAGGA AGAGGAGTCA GCGGATTCTG 
GCAGCTGAAA CAGACAGACT TTCCTACGTG GGAAACAATT TCGGGACTGG AGCCCTCAAA 
TGCAACACTT TGTGCAGGCA CTTTGTGGGA ATTCTCAACA AGACCTCTGG CCAGATGCAA 
GTGTATGATG CTGAATTGTT CAACATGCAG CCACTGTTTG CAGATGAATC ACTTGAGAGC 
GACTTGACAC AAGAGAGTCA GGCCAAAACT TTTAGAGAAA AGATGGATTC TTGCATTGAA 
GCCTTTGGAA CCACCAAACA GAAGCGGGCT TTGAACTCCA GAAGAATGAA CACAGTTGGC 
AGTGAATCTC TGAACTGTGC AGTAGCTAAA GCTGCAGAGA ATATTATTGA TGCGAAGGGT 
GTAACTGCTC TGGTCAACGA TGCCATCCAT GATGATCTGC AGGATGACTC CCTCTACCTT 
CCTCCCTGCC ACGCTGATGC AGCCAAGCCT GAAGACGTAT ATAAATTTGA AGACCTTCTG 
TCCACTGCGG AGTATGAAGC TCTTCAGAGC CCATCTGAAG CTTTCAGGAA TGTCTCATCA 
GAGGAAATAC TGAAGATGAT TGAAGAGAAC AGCCACTGCT CCTTTGTCAT AGAAGCATTG 
AAGTCTTTGC CATCAGATGA GAAGAGCCGA GACCGCCAGG CCCGATGCAT ATGGTTTCTG 
GACACGCTCA TCAAATTTCG AGCACAGAAA GTGGTTAAGC GGAAAAGTGC CCTGGGACCC 
GGAATCCCCC ATGTCATCAA CACCAAATTG CTGAAGCACT TCACCTGCTT GACCTACAAC 
AACGGCAGCC TACGGAACTT AATTTCGGAT TCTATGAAGG CGAAGATCAC TGCATATGTA 
ATCATACTTG CCTTGCACAT AAGCGACTTC CAGATTGACC TGACAATGTT ACAGAGGGAC 
TTGAAGCTTA GTGAGAGAAG GATGATTGAG ATAGCGAAAG CCATGAGGCT GAAGATCTCT 
AAAAGAAAGG TGTCTCTGGC AGCTGGCAGG GAAGAGGATC ACAAACTGGG CACCCTGTCC 
ATCCCGCTGC CTCCAGCCCA GACCTCAAAA CACCAGTCAA AGCGGAGGAA AATCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_531998.2
CDS	125..1384
Translation	MAAEVLPSARWLYCGQPDESQRAVLVQFSNGKLQNPGNVRFTLYKSNDSTNPRKRSQRILAAETDRLSYVGNNFGTGALKCNTLCRHFVGILNKTSGQMQVYDAELFNMQPLFADESLESDLTQESQAKTFREKMDSCIEAFGTTKQKRALNSRRMNTVGSESLNCAVAKAAENIIDAKGVTALVNDAIHDDLQDDSLYLPPCHADAAKPEDVYKFEDLLSTAEYEALQSPSEAFRNVSSEEILKMIEENSHCSFVIEALKSLPSDEKSRDRQARCIWFLDTLIKFRAQKVVKRKSALGPGIPHVINTKLLKHFTCLTYNNGSLRNLISDSMKAKITAYVIILALHISDFQIDLTMLQRDLKLSERRMIEIAKAMRLKISKRKVSLAAGREEDHKLGTLSIPLPPAQTSKHQSKRRKIT

Target ORF information:

RefSeq Version	XM_531998.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_531998.4

ORF Insert Sequence:

ATGGCGGCGG AGGTGTTGCC GAGTGCGCGG TGGCTATACT GTGGGCAGCC CGACGAGAGC 
CAGAGAGCCG TACTGGTGCA GTTCTCCAAC GGGAAGCTGC AGAATCCAGG CAACGTGCGT 
TTTACTTTGT ACAAGAGCAA TGACTCCACA AACCCCAGGA AGAGGAGTCA GCGGATTCTG 
GCAGCTGAAA CAGACAGACT TTCCTACGTG GGAAACAATT TCGGGACTGG AGCCCTCAAA 
TGCAACACTT TGTGCAGGCA CTTTGTGGGA ATTCTCAACA AGACCTCTGG CCAGATGCAA 
GTGTATGATG CTGAATTGTT CAACATGCAG CCACTGTTTG CAGATGAATC ACTTGAGAGC 
GACTTGACAC AAGAGAGTCA GGCCAAAACT TTTAGAGAAA AGATGGATTC TTGCATTGAA 
GCCTTTGGAA CCACCAAACA GAAGCGGGCT TTGAACTCCA GAAGAATGAA CACAGTTGGC 
AGTGAATCTC TGAACTGTGC AGTAGCTAAA GCTGCAGAGA ATATTATTGA TGCGAAGGGT 
GTAACTGCTC TGGTCAACGA TGCCATCCAT GATGATCTGC AGGATGACTC CCTCTACCTT 
CCTCCCTGCC ACGCTGATGC AGCCAAGCCT GAAGACGTAT ATAAATTTGA AGACCTTCTG 
TCCACTGCGG AGTATGAAGC TCTTCAGAGC CCATCTGAAG CTTTCAGGAA TGTCTCATCA 
GAGGAAATAC TGAAGATGAT TGAAGAGAAC AGCCACTGCT CCTTTGTCAT AGAAGCATTG 
AAGTCTTTGC CATCAGATGA GAAGAGCCGA GACCGCCAGG CCCGATGCAT ATGGTTTCTG 
GACACGCTCA TCAAATTTCG AGCACAGAAA GTGGTTAAGC GGAAAAGTGC CCTGGGACCC 
GGAATCCCCC ATGTCATCAA CACCAAATTG CTGAAGCACT TCACCTGCTT GACCTACAAC 
AACGGCAGCC TACGGAACTT AATTTCGGAT TCTATGAAGG CGAAGATCAC TGCATATGTA 
ATCATACTTG CCTTGCACAT AAGCGACTTC CAGATTGACC TGACAATGTT ACAGAGGGAC 
TTGAAGCTTA GTGAGAGAAG GATGATTGAG ATAGCGAAAG CCATGAGGCT GAAGATCTCT 
AAAAGAAAGG TGTCTCTGGC AGCTGGCAGG GAAGAGGATC ACAAACTGGG CACCCTGTCC 
ATCCCGCTGC CTCCAGCCCA GACCTCAAAA CACCAGTCAA AGCGGAGGAA AATCACCTAG