CD3E cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD3E
Entrez Gene ID	442981
Full Name	CD3e molecule
General protein information	Preferred Names CD3e molecule Names T-cell surface glycoprotein CD3 epsilon chain CD3 epsilon CD3E antigen, epsilon polypeptide (TiT3 complex)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003379.1	NP_001003379.1	T-cell surface glycoprotein CD3 epsilon chain precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CD3E	NP_996787.1
Homo sapiens (human)	CD3E	NP_000724.1
Canis lupus familiaris (dog)	CD3E	NP_001003379.1
Mus musculus (house mouse)	Cd3e	NP_031674.1
Xenopus tropicalis (tropical clawed frog)	cd3e	XP_002932946.1
Pan troglodytes (chimpanzee)	CD3E	XP_001160742.1
	CD3E	XP_001097204.1
Bos taurus (cattle)	CD3E	NP_776436.1
Rattus norvegicus (Norway rat)	Cd3e	NP_001101610.1

Gene Ontology
Bibliography

Related articles in PubMed

Tenascin-C in chronic canine hepatitis: immunohistochemical localization and correlation with necro-inflammatory activity, fibrotic stage, and expression of alpha-smooth muscle actin, cytokeratin 7, and CD3+ cells.
Mekonnen GA, Ijzer J, Nederbragt H
Veterinary pathology44(6)803-13(2007 Nov)

Molecular cloning of the CD3 epsilon subunit of the T-cell receptor/CD3 complex in dog.
Nash RA, Scherf U, Storb R
Immunogenetics33(5-6)396-8(1991)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reported the correlation of tenascin-C with the inflammatory activity and expression of alpha-smooth muscle actin (alpha-SMA), cytokeratin 7 (CK7), and CD3+ T-lymphocytes in canine chronic hepatitis.
Title: Tenascin-C in chronic canine hepatitis: immunohistochemical localization and correlation with necro-inflammatory activity, fibrotic stage, and expression of alpha-smooth muscle actin, cytokeratin 7, and CD3+ cells.

The following CD3E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD3E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00049	NM_001003379.1 Latest version!	Canis lupus familiaris CD3e molecule (CD3E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD3E ( NM_001003379.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003379.1 Canis lupus familiaris CD3e molecule (CD3E), mRNA.

Price & Availability↑

CloneID	OCe00049
Clone ID Related Accession (Same CDS sequence)	NM_001003379.1
Accession Version	NM_001003379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Canis lupus familiaris(dog)
Product	T-cell surface glycoprotein CD3 epsilon chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M55410.1. ##Evidence-Data-START## CDS exon combination :: M55410.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00991610 [ECO:0000350] ##Evidence-Data-END##

ATGCAGTCGA GGAACCTCTG GAGAATTCTG GGACTCTGTC TCTTATCAGT TGGTGCTTGG 
GGGCAGGACG AGGATTTCAA AGCTTCTGAT GACTTGACAA GTATATCTCC AGAGAAACGG 
TTTAAGGTCT CCATCTCTGG AACCGAGGTA GTGGTGACAT GCCCTGATGT TTTTGGATAT 
GATAATATAA AATGGGAAAA AAATGATAAC CTTGTGGAAG GTGCTAGTAA CAGAGAGCTA 
TCTCAGAAGG AGTTTTCAGA AGTGGACGAC AGTGGTTATT ATGCCTGCTA TGCAGATTCC 
ATAAAGGAGA AGAGCTATCT CTACCTGAGA GCAAGAGTGT GTGCAAACTG CATAGAGGTG 
AATCTGATGG CAGTGGTCAC AATCATTGTA GCTGACATCT GCCTTACTCT GGGGTTGCTG 
CTGATGGTGT ATTACTGGAG CAAGACTAGA AAGGCCAATG CCAAGCCTGT GATGAGAGGA 
ACAGGTGCCG GCAGCAGGCC CAGGGGACAA AACAAGGAGA AGCCACCACC TGTTCCCAAT 
CCAGACTACG AGCCCATCCG GAAAGGCCAG CAGGACCTGT ATTCTGGCCT GAATCAGAGA 
GGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003379.1
CDS	240..848
Translation	MQSRNLWRILGLCLLSVGAWGQDEDFKASDDLTSISPEKRFKVSISGTEVVVTCPDVFGYDNIKWEKNDNLVEGASNRELSQKEFSEVDDSGYYACYADSIKEKSYLYLRARVCANCIEVNLMAVVTIIVADICLTLGLLLMVYYWSKTRKANAKPVMRGTGAGSRPRGQNKEKPPPVPNPDYEPIRKGQQDLYSGLNQRGI

Target ORF information:

RefSeq Version	NM_001003379.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CD3e molecule (CD3E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003379.1

ORF Insert Sequence:

ATGCAGTCGA GGAACCTCTG GAGAATTCTG GGACTCTGTC TCTTATCAGT TGGTGCTTGG 
GGGCAGGACG AGGATTTCAA AGCTTCTGAT GACTTGACAA GTATATCTCC AGAGAAACGG 
TTTAAGGTCT CCATCTCTGG AACCGAGGTA GTGGTGACAT GCCCTGATGT TTTTGGATAT 
GATAATATAA AATGGGAAAA AAATGATAAC CTTGTGGAAG GTGCTAGTAA CAGAGAGCTA 
TCTCAGAAGG AGTTTTCAGA AGTGGACGAC AGTGGTTATT ATGCCTGCTA TGCAGATTCC 
ATAAAGGAGA AGAGCTATCT CTACCTGAGA GCAAGAGTGT GTGCAAACTG CATAGAGGTG 
AATCTGATGG CAGTGGTCAC AATCATTGTA GCTGACATCT GCCTTACTCT GGGGTTGCTG 
CTGATGGTGT ATTACTGGAG CAAGACTAGA AAGGCCAATG CCAAGCCTGT GATGAGAGGA 
ACAGGTGCCG GCAGCAGGCC CAGGGGACAA AACAAGGAGA AGCCACCACC TGTTCCCAAT 
CCAGACTACG AGCCCATCCG GAAAGGCCAG CAGGACCTGT ATTCTGGCCT GAATCAGAGA 
GGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tenascin-C in chronic canine hepatitis: immunohistochemical localization and correlation with necro-inflammatory activity, fibrotic stage, and expression of alpha-smooth muscle actin, cytokeratin 7, and CD3+ cells.
Veterinary pathology44(6)803-13(2007 Nov)
Mekonnen GA,Ijzer J,Nederbragt H

Molecular cloning of the CD3 epsilon subunit of the T-cell receptor/CD3 complex in dog.
Immunogenetics33(5-6)396-8(1991)
Nash RA,Scherf U,Storb R