PLN cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLN
Entrez Gene ID	414755
Full Name	phospholamban
General protein information	Preferred Names phospholamban Names cardiac phospholamban PLB
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003332.1	NP_001003332.1	cardiac phospholamban
XM_014116918.2	XP_013972393.1	cardiac phospholamban isoform X1
XM_014116918.1	XP_013972393.1	cardiac phospholamban isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pln	NP_073198.1
	PLN	XP_002803964.1
Canis lupus familiaris (dog)	PLN	NP_001003332.1
Bos taurus (cattle)	PLN	NP_001096789.1
Mus musculus (house mouse)	Pln	NP_001135399.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardiac gene transfer of short hairpin RNA directed against phospholamban effectively knocks down gene expression but causes cellular toxicity in canines.
Bish LT, Sleeper MM, Reynolds C, Gazzara J, Withnall E, Singletary GE, Buchlis G, Hui D, High KA, Gao G, Wilson JM, Sweeney HL
Human gene therapy22(8)969-77(2011 Aug)

Superinhibitory phospholamban mutants compete with Ca2+ for binding to SERCA2a by stabilizing a unique nucleotide-dependent conformational state.
Akin BL, Chen Z, Jones LR
The Journal of biological chemistry285(37)28540-52(2010 Sep)

Ca2+ binding to site I of the cardiac Ca2+ pump is sufficient to dissociate phospholamban.
Chen Z, Akin BL, Jones LR
The Journal of biological chemistry285(5)3253-60(2010 Jan)

Phospholamban thiols play a central role in activation of the cardiac muscle sarcoplasmic reticulum calcium pump by nitroxyl.
Froehlich JP, Mahaney JE, Keceli G, Pavlos CM, Goldstein R, Redwood AJ, Sumbilla C, Lee DI, Tocchetti CG, Kass DA, Paolocci N, Toscano JP
Biochemistry47(50)13150-2(2008 Dec)

Phospholamban oligomerization, quaternary structure, and sarco(endo)plasmic reticulum calcium ATPase binding measured by fluorescence resonance energy transfer in living cells.
Kelly EM, Hou Z, Bossuyt J, Bers DM, Robia SL
The Journal of biological chemistry283(18)12202-11(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PLB mRNA was reduced 16-fold, and PLB protein was reduced 5-fold.
Title: Cardiac gene transfer of short hairpin RNA directed against phospholamban effectively knocks down gene expression but causes cellular toxicity in canines.

PLB decreases Ca(2+) binding to SERCA2a by stabilizing a unique E2.ATP state that is unable to bind thapsigargin or vanadate.
Title: Superinhibitory phospholamban mutants compete with Ca2+ for binding to SERCA2a by stabilizing a unique nucleotide-dependent conformational state.

Ca2+ binding to site I of the cardiac Ca2+ pump is sufficient to dissociate phospholamban
Title: Ca2+ binding to site I of the cardiac Ca2+ pump is sufficient to dissociate phospholamban.

Activation of cardiac muscle sarcoplasmic reticulum calcium-transporting ATPase (SERCA2a) dephosphorylation by nitroxyl (HNO) is PLN-dependent; PLN thiols are targets for HNO.
Title: Phospholamban thiols play a central role in activation of the cardiac muscle sarcoplasmic reticulum calcium pump by nitroxyl.

Phospholamban (PLB) oligomerization, quaternary structure, and sarco(endo)plasmic reticulum calcium ATPase (SERCA) binding were quantified by fluorescence resonance energy transfer (FRET) in an intact cellular environment.
Title: Phospholamban oligomerization, quaternary structure, and sarco(endo)plasmic reticulum calcium ATPase binding measured by fluorescence resonance energy transfer in living cells.

The following PLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00172	NM_001003332.1 Latest version!	Canis lupus familiaris phospholamban (PLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe00172	XM_014116918.2 Latest version!	Canis lupus familiaris phospholamban (PLN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OCe00172	XM_014116918.1	Canis lupus familiaris phospholamban (PLN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLN ( NM_001003332.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> NP_001003332.1 Canis lupus familiaris phospholamban (PLN), mRNA.

Price & Availability↑

CloneID	OCe00172
Clone ID Related Accession (Same CDS sequence)	NM_001003332.1 , XM_014116918.2 , XM_014116918.1
Accession Version	NM_001003332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	cardiac phospholamban
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y00399.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00399.1, CX011747.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

1
61
121

ATGGATAAAG TCCAATACCT CACTCGCTCT GCTATTAGAA GAGCTTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA TCTTCAGAAC CTATTTATAA ATTTCTGTCT CATTTTAATA 
TGTCTCTTGT TGATCTGCAT CATTGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003332.1
CDS	185..343
Translation	MDKVQYLTRSAIRRASTIEMPQQARQNLQNLFINFCLILICLLLICIIVMLL

Target ORF information:

RefSeq Version	NM_001003332.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholamban (PLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003332.1

ORF Insert Sequence:

1
61
121

ATGGATAAAG TCCAATACCT CACTCGCTCT GCTATTAGAA GAGCTTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA TCTTCAGAAC CTATTTATAA ATTTCTGTCT CATTTTAATA 
TGTCTCTTGT TGATCTGCAT CATTGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLN ( XM_014116918.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972393.1 Canis lupus familiaris phospholamban (PLN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe00172
Clone ID Related Accession (Same CDS sequence)	NM_001003332.1 , XM_014116918.2 , XM_014116918.1
Accession Version	XM_014116918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cardiac phospholamban isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121

ATGGATAAAG TCCAATACCT CACTCGCTCT GCTATTAGAA GAGCTTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA TCTTCAGAAC CTATTTATAA ATTTCTGTCT CATTTTAATA 
TGTCTCTTGT TGATCTGCAT CATTGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972393.1
CDS	379..537
Translation	MDKVQYLTRSAIRRASTIEMPQQARQNLQNLFINFCLILICLLLICIIVMLL

Target ORF information:

RefSeq Version	XM_014116918.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholamban (PLN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116918.2

ORF Insert Sequence:

1
61
121

ATGGATAAAG TCCAATACCT CACTCGCTCT GCTATTAGAA GAGCTTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA TCTTCAGAAC CTATTTATAA ATTTCTGTCT CATTTTAATA 
TGTCTCTTGT TGATCTGCAT CATTGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLN ( XM_014116918.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013972393.1 Canis lupus familiaris phospholamban (PLN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe00172
Clone ID Related Accession (Same CDS sequence)	NM_001003332.1 , XM_014116918.2 , XM_014116918.1
Accession Version	XM_014116918.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cardiac phospholamban isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121

ATGGATAAAG TCCAATACCT CACTCGCTCT GCTATTAGAA GAGCTTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA TCTTCAGAAC CTATTTATAA ATTTCTGTCT CATTTTAATA 
TGTCTCTTGT TGATCTGCAT CATTGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972393.1
CDS	361..519
Translation	MDKVQYLTRSAIRRASTIEMPQQARQNLQNLFINFCLILICLLLICIIVMLL

Target ORF information:

RefSeq Version	XM_014116918.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholamban (PLN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116918.1

ORF Insert Sequence:

1
61
121

ATGGATAAAG TCCAATACCT CACTCGCTCT GCTATTAGAA GAGCTTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA TCTTCAGAAC CTATTTATAA ATTTCTGTCT CATTTTAATA 
TGTCTCTTGT TGATCTGCAT CATTGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiac gene transfer of short hairpin RNA directed against phospholamban effectively knocks down gene expression but causes cellular toxicity in canines.
Human gene therapy22(8)969-77(2011 Aug)
Bish LT,Sleeper MM,Reynolds C,Gazzara J,Withnall E,Singletary GE,Buchlis G,Hui D,High KA,Gao G,Wilson JM,Sweeney HL

Superinhibitory phospholamban mutants compete with Ca2+ for binding to SERCA2a by stabilizing a unique nucleotide-dependent conformational state.
The Journal of biological chemistry285(37)28540-52(2010 Sep)
Akin BL,Chen Z,Jones LR

Ca2+ binding to site I of the cardiac Ca2+ pump is sufficient to dissociate phospholamban.
The Journal of biological chemistry285(5)3253-60(2010 Jan)
Chen Z,Akin BL,Jones LR

Phospholamban thiols play a central role in activation of the cardiac muscle sarcoplasmic reticulum calcium pump by nitroxyl.
Biochemistry47(50)13150-2(2008 Dec)
Froehlich JP,Mahaney JE,Keceli G,Pavlos CM,Goldstein R,Redwood AJ,Sumbilla C,Lee DI,Tocchetti CG,Kass DA,Paolocci N,Toscano JP

Phospholamban oligomerization, quaternary structure, and sarco(endo)plasmic reticulum calcium ATPase binding measured by fluorescence resonance energy transfer in living cells.
The Journal of biological chemistry283(18)12202-11(2008 May)
Kelly EM,Hou Z,Bossuyt J,Bers DM,Robia SL