PDE5A gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014109885.1	XP_013965360.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
XM_005639043.2	XP_005639100.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
XM_005639046.2	XP_005639103.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
XM_022413385.1	XP_022269093.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
XM_005639044.1	XP_005639101.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
NM_001003188.1	NP_001003188.1	cGMP-specific 3',5'-cyclic phosphodiesterase
XM_005639045.3	XP_005639102.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
XM_014109885.2	XP_013965360.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
XM_005639045.2	XP_005639102.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
XM_005639043.3	XP_005639100.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe34253	NM_001003188.1 Latest version!	Canis lupus familiaris phosphodiesterase 5A (PDE5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe29597	XM_005639045.3 Latest version!	Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29597	XM_005639046.2 Latest version!	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe284003	XM_022413385.1 Latest version!	Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe29595	XM_005639043.3 Latest version!	Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe29596	XM_005639044.1 Latest version!	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe44532	XM_014109885.2 Latest version!	Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OCe44532	XM_014109885.1	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe29597	XM_005639045.2	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe29595	XM_005639043.2	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

PDE5A ( NM_001003188.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003188.1 Canis lupus familiaris phosphodiesterase 5A (PDE5A), mRNA.

Price & Availability↑

CloneID	OCe34253
Clone ID Related Accession (Same CDS sequence)	NM_001003188.1
Accession Version	NM_001003188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Canis lupus familiaris(dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB008468.1. ##Evidence-Data-START## Transcript exon combination :: AB008468.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGTTGCCCT TTGGACACCA AAGAAGAGAA ATGGTCAATG CCTGGTTCGC TGAGAGAGTC 
CACACCATCC CTGTGTGTAA AGAAGGCATC AGAGGCCACG CAGAATCTTG CTCTTGCTCC 
TCGCAGCAGA GTTCCCGTGC AGACAGCAGT GCGCCTGGCA CACCAACCAG GAAAATCTCT 
GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTCGTCA AAGATTCTGA GGGTACTGTG 
AGCTTCCTCG CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCCCC AAGGTTTGAT 
AATGATGAAG GGGACCAGTG CTCGAGACTC TTGGAATTAG TGAAAGATAT TTCTAGTCAT 
TTGGATGTCA CAGCCTTATG TCATAAAATT TTCTTGCATA TTCATGGACT TATCTCCGCT 
GATCGCTATT CCCTGTTTCT TGTCTGTGAG GACAGCTCCA ATGACAAGTT TCTTATCAGC 
CGCCTGTTTG ATGTTGCAGA AGGTTCAACA CTGGAAGAAG CTTCAAATAA CTGTATCCGC 
TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCAC TTGGTGAGCC CTTGAACATC 
AAAGATGCTT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGTTACAAG 
ACACAGAGTA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACATTCA CTGAAAAAGA TGAAAAGGAC 
TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAACT CTATGAGACT 
TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGCTT AATTTTTGAA 
GAACAACAAT CATTAGAAGT AATTCTAAAG AAAATAGCTG CCACTATTAT CTCTTTCATG 
CAGGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGC 
GTCTTTCACA TGGAATGTGA GGAATTAGAA AAATTGCCAG ATACGTTAAC AAGGGAACGT 
GATGCAAACA GAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT 
AATATCCCAG ATGTCAGTAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CACAGGAAAT 
GTAAACCAGC AATGCATTAG AAGTTTGCTT TGTACTCCTA TAAAAAATGG AAAGAAGAAT 
AAAGTGATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG 
CCTTTCAACC GAAATGATGA ACAGTTTCTG GAGGCTTTTG TCATCTTTTG CGGCTTGGGG 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA GATGGTCACA 
CTGGAGGTTC TATCCTACCA TGCTTCAGCA GCAGAGGAGG AAACAAAAGA GCTCCAATCC 
TTGGCGGCTG CTGTGGTACC ATCTGCTCAG ACGCTTAAAA TTACTGACTT CAGCTTCAGT 
GACTTTGAGC TGTCTGACCT AGAAACAGCA CTGTGCACAA TTCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTT 
AAGAAGAATT ACAGGAAGAA TGTTGCCTAT CATAATTGGA GACACGCCTT TAATACAGCT 
CAGTGCATGT TTGCTGCTTT AAAGGCCGGC AAGATTCAGA ACAAGCTGAC TGACCTGGAG 
ATACTTGCCC TGCTGATTGC TGCTCTCAGC CACGACTTGG ACCACCGTGG TGTGAATAAC 
TCTTATATAC AGCGAAGTGA ACACCCACTT GCTCAACTCT ACTGCCATTC AATCATGGAG 
CATCATCATT TTGACCAGTG CCTGATGATT CTCAATAGTC CAGGCAATCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAATA TAAGACCACG CTGAAAATAA TCAAGCAAGC TATTTTAGCC 
ACAGACCTAG CATTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT 
CAATTCAACT TGGAAGATCC TCATCAAAAA GAGTTGTTTT TAGCGATGCT GATGACAGCT 
TGTGACCTGT CTGCCATTAC AAAACCGTGG CCTATTCAAC AACGGATAGC AGAACTTGTA 
GCAACTGAAT TTTTTGACCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAGCCTGCT 
GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAACTATA TGAGGCTCTG ACCCACGTAT CAGAGGACTG CTTCCCTTTG 
CTGGATGGCT GCAGAAAGAA CAGGCAAAAA TGGCAGGCCC TAGCAGAACA GCAGGAGAAA 
ACGCTGATTA ATGGGGAAAG CAGCCAGGCC AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003188.1
CDS	118..2619
Translation	MLPFGHQRREMVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	NM_001003188.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphodiesterase 5A (PDE5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003188.1

ORF Insert Sequence:

ATGTTGCCCT TTGGACACCA AAGAAGAGAA ATGGTCAATG CCTGGTTCGC TGAGAGAGTC 
CACACCATCC CTGTGTGTAA AGAAGGCATC AGAGGCCACG CAGAATCTTG CTCTTGCTCC 
TCGCAGCAGA GTTCCCGTGC AGACAGCAGT GCGCCTGGCA CACCAACCAG GAAAATCTCT 
GCCTCTGAAT TTGACCGGCC TCTTAGACCC ATTGTCGTCA AAGATTCTGA GGGTACTGTG 
AGCTTCCTCG CTGACTCAGA AAAGAAGGAA CAGATGCCTC TAACCCCCCC AAGGTTTGAT 
AATGATGAAG GGGACCAGTG CTCGAGACTC TTGGAATTAG TGAAAGATAT TTCTAGTCAT 
TTGGATGTCA CAGCCTTATG TCATAAAATT TTCTTGCATA TTCATGGACT TATCTCCGCT 
GATCGCTATT CCCTGTTTCT TGTCTGTGAG GACAGCTCCA ATGACAAGTT TCTTATCAGC 
CGCCTGTTTG ATGTTGCAGA AGGTTCAACA CTGGAAGAAG CTTCAAATAA CTGTATCCGC 
TTAGAATGGA ACAAAGGCAT TGTGGGACAT GTGGCAGCAC TTGGTGAGCC CTTGAACATC 
AAAGATGCTT ATGAGGATCC TCGGTTCAAT GCAGAAGTTG ACCAAATTAC AGGTTACAAG 
ACACAGAGTA TTCTTTGTAT GCCAATTAAG AATCATAGGG AAGAGGTTGT TGGTGTAGCC 
CAGGCCATCA ACAAGAAATC AGGAAATGGT GGGACATTCA CTGAAAAAGA TGAAAAGGAC 
TTTGCTGCTT ATTTGGCATT TTGTGGTATT GTTCTTCATA ATGCTCAACT CTATGAGACT 
TCACTGCTGG AGAACAAGAG AAATCAGGTG CTGCTTGACC TTGCTAGCTT AATTTTTGAA 
GAACAACAAT CATTAGAAGT AATTCTAAAG AAAATAGCTG CCACTATTAT CTCTTTCATG 
CAGGTGCAGA AATGCACCAT TTTCATAGTG GATGAAGATT GCTCCGATTC TTTTTCTAGC 
GTCTTTCACA TGGAATGTGA GGAATTAGAA AAATTGCCAG ATACGTTAAC AAGGGAACGT 
GATGCAAACA GAATCAATTA CATGTATGCT CAGTATGTCA AAAATACTAT GGAACCACTT 
AATATCCCAG ATGTCAGTAA GGACAAAAGA TTTCCCTGGA CAAATGAAAA CACAGGAAAT 
GTAAACCAGC AATGCATTAG AAGTTTGCTT TGTACTCCTA TAAAAAATGG AAAGAAGAAT 
AAAGTGATAG GGGTTTGCCA ACTTGTTAAT AAGATGGAGG AGAATACTGG CAAGGTTAAG 
CCTTTCAACC GAAATGATGA ACAGTTTCTG GAGGCTTTTG TCATCTTTTG CGGCTTGGGG 
ATCCAGAACA CACAGATGTA TGAAGCAGTG GAGAGAGCCA TGGCCAAGCA GATGGTCACA 
CTGGAGGTTC TATCCTACCA TGCTTCAGCA GCAGAGGAGG AAACAAAAGA GCTCCAATCC 
TTGGCGGCTG CTGTGGTACC ATCTGCTCAG ACGCTTAAAA TTACTGACTT CAGCTTCAGT 
GACTTTGAGC TGTCTGACCT AGAAACAGCA CTGTGCACAA TTCGGATGTT CACTGACCTC 
AACCTTGTGC AGAACTTCCA GATGAAACAT GAGGTTCTTT GCAGGTGGAT TTTAAGTGTT 
AAGAAGAATT ACAGGAAGAA TGTTGCCTAT CATAATTGGA GACACGCCTT TAATACAGCT 
CAGTGCATGT TTGCTGCTTT AAAGGCCGGC AAGATTCAGA ACAAGCTGAC TGACCTGGAG 
ATACTTGCCC TGCTGATTGC TGCTCTCAGC CACGACTTGG ACCACCGTGG TGTGAATAAC 
TCTTATATAC AGCGAAGTGA ACACCCACTT GCTCAACTCT ACTGCCATTC AATCATGGAG 
CATCATCATT TTGACCAGTG CCTGATGATT CTCAATAGTC CAGGCAATCA GATTCTCAGT 
GGCCTCTCCA TTGAAGAATA TAAGACCACG CTGAAAATAA TCAAGCAAGC TATTTTAGCC 
ACAGACCTAG CATTGTACAT TAAGAGACGA GGAGAATTTT TTGAACTTAT AAGAAAAAAT 
CAATTCAACT TGGAAGATCC TCATCAAAAA GAGTTGTTTT TAGCGATGCT GATGACAGCT 
TGTGACCTGT CTGCCATTAC AAAACCGTGG CCTATTCAAC AACGGATAGC AGAACTTGTA 
GCAACTGAAT TTTTTGACCA AGGAGACAGA GAGAGAAAAG AACTCAACAT AGAGCCTGCT 
GATCTAATGA ACAGGGAGAA GAAAAACAAA ATCCCAAGTA TGCAAGTTGG GTTCATAGAT 
GCCATCTGCT TGCAACTATA TGAGGCTCTG ACCCACGTAT CAGAGGACTG CTTCCCTTTG 
CTGGATGGCT GCAGAAAGAA CAGGCAAAAA TGGCAGGCCC TAGCAGAACA GCAGGAGAAA 
ACGCTGATTA ATGGGGAAAG CAGCCAGGCC AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_005639046.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005639103.1 Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe29597
Clone ID Related Accession (Same CDS sequence)	XM_005639046.2 , XM_005639045.3 , XM_005639045.2
Accession Version	XM_005639046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATG CCTGGTTCGC TGAGAGAGTC CACACCATCC CTGTGTGTAA AGAAGGCATC 
AGAGGCCACG CAGAATCTTG CTCTTGCTCC TCGCAGCAGA GTTCCCGTGC AGACAGCAGT 
GCGCCTGGCA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC 
ATTGTCGTCA AAGATTCTGA GGGTACTGTG AGCTTCCTCG CTGACTCAGA AAAGAAGGAA 
CAGATGCCTC TAACCCCCCC AAGGTTTGAT AATGATGAAG GGGACCAGTG CTCGAGACTC 
TTGGAATTAG TGAAAGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCATAAAATT 
TTCTTGCATA TTCATGGACT TATCTCCGCT GATCGCTATT CCCTGTTTCT TGTCTGTGAG 
GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTGTTTG ATGTTGCAGA AGGTTCAACA 
CTGGAAGAAG CTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT 
GTGGCAGCAC TTGGTGAGCC CTTGAACATC AAAGATGCTT ATGAGGATCC TCGGTTCAAT 
GCAGAAGTTG ACCAAATTAC AGGTTACAAG ACACAGAGTA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAATGGT 
GGGACATTCA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT 
GTTCTTCATA ATGCTCAACT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGCTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTCTAAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAGGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGC GTCTTTCACA TGGAATGTGA GGAATTAGAA 
AAATTGCCAG ATACGTTAAC AAGGGAACGT GATGCAAACA GAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGACAAAAGA 
TTTCCCTGGA CAAATGAAAA CACAGGAAAT GTAAACCAGC AATGCATTAG AAGTTTGCTT 
TGTACTCCTA TAAAAAATGG AAAGAAGAAT AAAGTGATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGATGA ACAGTTTCTG 
GAGGCTTTTG TCATCTTTTG CGGCTTGGGG ATCCAGAACA CACAGATGTA TGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA GATGGTCACA CTGGAGGTTC TATCCTACCA TGCTTCAGCA 
GCAGAGGAGG AAACAAAAGA GCTCCAATCC TTGGCGGCTG CTGTGGTACC ATCTGCTCAG 
ACGCTTAAAA TTACTGACTT CAGCTTCAGT GACTTTGAGC TGTCTGACCT AGAAACAGCA 
CTGTGCACAA TTCGGATGTT CACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTTCTTT GCAGGTGGAT TTTAAGTGTT AAGAAGAATT ACAGGAAGAA TGTTGCCTAT 
CATAATTGGA GACACGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTTT AAAGGCCGGC 
AAGATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCCC TGCTGATTGC TGCTCTCAGC 
CACGACTTGG ACCACCGTGG TGTGAATAAC TCTTATATAC AGCGAAGTGA ACACCCACTT 
GCTCAACTCT ACTGCCATTC AATCATGGAG CATCATCATT TTGACCAGTG CCTGATGATT 
CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG 
CTGAAAATAA TCAAGCAAGC TATTTTAGCC ACAGACCTAG CATTGTACAT TAAGAGACGA 
GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAACT TGGAAGATCC TCATCAAAAA 
GAGTTGTTTT TAGCGATGCT GATGACAGCT TGTGACCTGT CTGCCATTAC AAAACCGTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGACCA AGGAGACAGA 
GAGAGAAAAG AACTCAACAT AGAGCCTGCT GATCTAATGA ACAGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTATA TGAGGCTCTG 
ACCCACGTAT CAGAGGACTG CTTCCCTTTG CTGGATGGCT GCAGAAAGAA CAGGCAAAAA 
TGGCAGGCCC TAGCAGAACA GCAGGAGAAA ACGCTGATTA ATGGGGAAAG CAGCCAGGCC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639103.1
CDS	174..2645
Translation	MVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	XM_005639046.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639046.2

ORF Insert Sequence:

ATGGTCAATG CCTGGTTCGC TGAGAGAGTC CACACCATCC CTGTGTGTAA AGAAGGCATC 
AGAGGCCACG CAGAATCTTG CTCTTGCTCC TCGCAGCAGA GTTCCCGTGC AGACAGCAGT 
GCGCCTGGCA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC 
ATTGTCGTCA AAGATTCTGA GGGTACTGTG AGCTTCCTCG CTGACTCAGA AAAGAAGGAA 
CAGATGCCTC TAACCCCCCC AAGGTTTGAT AATGATGAAG GGGACCAGTG CTCGAGACTC 
TTGGAATTAG TGAAAGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCATAAAATT 
TTCTTGCATA TTCATGGACT TATCTCCGCT GATCGCTATT CCCTGTTTCT TGTCTGTGAG 
GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTGTTTG ATGTTGCAGA AGGTTCAACA 
CTGGAAGAAG CTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT 
GTGGCAGCAC TTGGTGAGCC CTTGAACATC AAAGATGCTT ATGAGGATCC TCGGTTCAAT 
GCAGAAGTTG ACCAAATTAC AGGTTACAAG ACACAGAGTA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAATGGT 
GGGACATTCA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT 
GTTCTTCATA ATGCTCAACT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGCTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTCTAAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAGGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGC GTCTTTCACA TGGAATGTGA GGAATTAGAA 
AAATTGCCAG ATACGTTAAC AAGGGAACGT GATGCAAACA GAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGACAAAAGA 
TTTCCCTGGA CAAATGAAAA CACAGGAAAT GTAAACCAGC AATGCATTAG AAGTTTGCTT 
TGTACTCCTA TAAAAAATGG AAAGAAGAAT AAAGTGATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGATGA ACAGTTTCTG 
GAGGCTTTTG TCATCTTTTG CGGCTTGGGG ATCCAGAACA CACAGATGTA TGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA GATGGTCACA CTGGAGGTTC TATCCTACCA TGCTTCAGCA 
GCAGAGGAGG AAACAAAAGA GCTCCAATCC TTGGCGGCTG CTGTGGTACC ATCTGCTCAG 
ACGCTTAAAA TTACTGACTT CAGCTTCAGT GACTTTGAGC TGTCTGACCT AGAAACAGCA 
CTGTGCACAA TTCGGATGTT CACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTTCTTT GCAGGTGGAT TTTAAGTGTT AAGAAGAATT ACAGGAAGAA TGTTGCCTAT 
CATAATTGGA GACACGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTTT AAAGGCCGGC 
AAGATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCCC TGCTGATTGC TGCTCTCAGC 
CACGACTTGG ACCACCGTGG TGTGAATAAC TCTTATATAC AGCGAAGTGA ACACCCACTT 
GCTCAACTCT ACTGCCATTC AATCATGGAG CATCATCATT TTGACCAGTG CCTGATGATT 
CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG 
CTGAAAATAA TCAAGCAAGC TATTTTAGCC ACAGACCTAG CATTGTACAT TAAGAGACGA 
GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAACT TGGAAGATCC TCATCAAAAA 
GAGTTGTTTT TAGCGATGCT GATGACAGCT TGTGACCTGT CTGCCATTAC AAAACCGTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGACCA AGGAGACAGA 
GAGAGAAAAG AACTCAACAT AGAGCCTGCT GATCTAATGA ACAGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTATA TGAGGCTCTG 
ACCCACGTAT CAGAGGACTG CTTCCCTTTG CTGGATGGCT GCAGAAAGAA CAGGCAAAAA 
TGGCAGGCCC TAGCAGAACA GCAGGAGAAA ACGCTGATTA ATGGGGAAAG CAGCCAGGCC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_005639045.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005639102.1 Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe29597
Clone ID Related Accession (Same CDS sequence)	XM_005639046.2 , XM_005639045.3 , XM_005639045.2
Accession Version	XM_005639045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATG CCTGGTTCGC TGAGAGAGTC CACACCATCC CTGTGTGTAA AGAAGGCATC 
AGAGGCCACG CAGAATCTTG CTCTTGCTCC TCGCAGCAGA GTTCCCGTGC AGACAGCAGT 
GCGCCTGGCA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC 
ATTGTCGTCA AAGATTCTGA GGGTACTGTG AGCTTCCTCG CTGACTCAGA AAAGAAGGAA 
CAGATGCCTC TAACCCCCCC AAGGTTTGAT AATGATGAAG GGGACCAGTG CTCGAGACTC 
TTGGAATTAG TGAAAGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCATAAAATT 
TTCTTGCATA TTCATGGACT TATCTCCGCT GATCGCTATT CCCTGTTTCT TGTCTGTGAG 
GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTGTTTG ATGTTGCAGA AGGTTCAACA 
CTGGAAGAAG CTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT 
GTGGCAGCAC TTGGTGAGCC CTTGAACATC AAAGATGCTT ATGAGGATCC TCGGTTCAAT 
GCAGAAGTTG ACCAAATTAC AGGTTACAAG ACACAGAGTA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAATGGT 
GGGACATTCA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT 
GTTCTTCATA ATGCTCAACT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGCTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTCTAAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAGGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGC GTCTTTCACA TGGAATGTGA GGAATTAGAA 
AAATTGCCAG ATACGTTAAC AAGGGAACGT GATGCAAACA GAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGACAAAAGA 
TTTCCCTGGA CAAATGAAAA CACAGGAAAT GTAAACCAGC AATGCATTAG AAGTTTGCTT 
TGTACTCCTA TAAAAAATGG AAAGAAGAAT AAAGTGATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGATGA ACAGTTTCTG 
GAGGCTTTTG TCATCTTTTG CGGCTTGGGG ATCCAGAACA CACAGATGTA TGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA GATGGTCACA CTGGAGGTTC TATCCTACCA TGCTTCAGCA 
GCAGAGGAGG AAACAAAAGA GCTCCAATCC TTGGCGGCTG CTGTGGTACC ATCTGCTCAG 
ACGCTTAAAA TTACTGACTT CAGCTTCAGT GACTTTGAGC TGTCTGACCT AGAAACAGCA 
CTGTGCACAA TTCGGATGTT CACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTTCTTT GCAGGTGGAT TTTAAGTGTT AAGAAGAATT ACAGGAAGAA TGTTGCCTAT 
CATAATTGGA GACACGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTTT AAAGGCCGGC 
AAGATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCCC TGCTGATTGC TGCTCTCAGC 
CACGACTTGG ACCACCGTGG TGTGAATAAC TCTTATATAC AGCGAAGTGA ACACCCACTT 
GCTCAACTCT ACTGCCATTC AATCATGGAG CATCATCATT TTGACCAGTG CCTGATGATT 
CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG 
CTGAAAATAA TCAAGCAAGC TATTTTAGCC ACAGACCTAG CATTGTACAT TAAGAGACGA 
GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAACT TGGAAGATCC TCATCAAAAA 
GAGTTGTTTT TAGCGATGCT GATGACAGCT TGTGACCTGT CTGCCATTAC AAAACCGTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGACCA AGGAGACAGA 
GAGAGAAAAG AACTCAACAT AGAGCCTGCT GATCTAATGA ACAGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTATA TGAGGCTCTG 
ACCCACGTAT CAGAGGACTG CTTCCCTTTG CTGGATGGCT GCAGAAAGAA CAGGCAAAAA 
TGGCAGGCCC TAGCAGAACA GCAGGAGAAA ACGCTGATTA ATGGGGAAAG CAGCCAGGCC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639102.1
CDS	112..2583
Translation	MVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	XM_005639045.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639045.3

ORF Insert Sequence:

ATGGTCAATG CCTGGTTCGC TGAGAGAGTC CACACCATCC CTGTGTGTAA AGAAGGCATC 
AGAGGCCACG CAGAATCTTG CTCTTGCTCC TCGCAGCAGA GTTCCCGTGC AGACAGCAGT 
GCGCCTGGCA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC 
ATTGTCGTCA AAGATTCTGA GGGTACTGTG AGCTTCCTCG CTGACTCAGA AAAGAAGGAA 
CAGATGCCTC TAACCCCCCC AAGGTTTGAT AATGATGAAG GGGACCAGTG CTCGAGACTC 
TTGGAATTAG TGAAAGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCATAAAATT 
TTCTTGCATA TTCATGGACT TATCTCCGCT GATCGCTATT CCCTGTTTCT TGTCTGTGAG 
GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTGTTTG ATGTTGCAGA AGGTTCAACA 
CTGGAAGAAG CTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT 
GTGGCAGCAC TTGGTGAGCC CTTGAACATC AAAGATGCTT ATGAGGATCC TCGGTTCAAT 
GCAGAAGTTG ACCAAATTAC AGGTTACAAG ACACAGAGTA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAATGGT 
GGGACATTCA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT 
GTTCTTCATA ATGCTCAACT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGCTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTCTAAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAGGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGC GTCTTTCACA TGGAATGTGA GGAATTAGAA 
AAATTGCCAG ATACGTTAAC AAGGGAACGT GATGCAAACA GAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGACAAAAGA 
TTTCCCTGGA CAAATGAAAA CACAGGAAAT GTAAACCAGC AATGCATTAG AAGTTTGCTT 
TGTACTCCTA TAAAAAATGG AAAGAAGAAT AAAGTGATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGATGA ACAGTTTCTG 
GAGGCTTTTG TCATCTTTTG CGGCTTGGGG ATCCAGAACA CACAGATGTA TGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA GATGGTCACA CTGGAGGTTC TATCCTACCA TGCTTCAGCA 
GCAGAGGAGG AAACAAAAGA GCTCCAATCC TTGGCGGCTG CTGTGGTACC ATCTGCTCAG 
ACGCTTAAAA TTACTGACTT CAGCTTCAGT GACTTTGAGC TGTCTGACCT AGAAACAGCA 
CTGTGCACAA TTCGGATGTT CACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTTCTTT GCAGGTGGAT TTTAAGTGTT AAGAAGAATT ACAGGAAGAA TGTTGCCTAT 
CATAATTGGA GACACGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTTT AAAGGCCGGC 
AAGATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCCC TGCTGATTGC TGCTCTCAGC 
CACGACTTGG ACCACCGTGG TGTGAATAAC TCTTATATAC AGCGAAGTGA ACACCCACTT 
GCTCAACTCT ACTGCCATTC AATCATGGAG CATCATCATT TTGACCAGTG CCTGATGATT 
CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG 
CTGAAAATAA TCAAGCAAGC TATTTTAGCC ACAGACCTAG CATTGTACAT TAAGAGACGA 
GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAACT TGGAAGATCC TCATCAAAAA 
GAGTTGTTTT TAGCGATGCT GATGACAGCT TGTGACCTGT CTGCCATTAC AAAACCGTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGACCA AGGAGACAGA 
GAGAGAAAAG AACTCAACAT AGAGCCTGCT GATCTAATGA ACAGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTATA TGAGGCTCTG 
ACCCACGTAT CAGAGGACTG CTTCCCTTTG CTGGATGGCT GCAGAAAGAA CAGGCAAAAA 
TGGCAGGCCC TAGCAGAACA GCAGGAGAAA ACGCTGATTA ATGGGGAAAG CAGCCAGGCC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_005639045.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005639102.1 Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe29597
Clone ID Related Accession (Same CDS sequence)	XM_005639046.2 , XM_005639045.3 , XM_005639045.2
Accession Version	XM_005639045.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATG CCTGGTTCGC TGAGAGAGTC CACACCATCC CTGTGTGTAA AGAAGGCATC 
AGAGGCCACG CAGAATCTTG CTCTTGCTCC TCGCAGCAGA GTTCCCGTGC AGACAGCAGT 
GCGCCTGGCA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC 
ATTGTCGTCA AAGATTCTGA GGGTACTGTG AGCTTCCTCG CTGACTCAGA AAAGAAGGAA 
CAGATGCCTC TAACCCCCCC AAGGTTTGAT AATGATGAAG GGGACCAGTG CTCGAGACTC 
TTGGAATTAG TGAAAGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCATAAAATT 
TTCTTGCATA TTCATGGACT TATCTCCGCT GATCGCTATT CCCTGTTTCT TGTCTGTGAG 
GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTGTTTG ATGTTGCAGA AGGTTCAACA 
CTGGAAGAAG CTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT 
GTGGCAGCAC TTGGTGAGCC CTTGAACATC AAAGATGCTT ATGAGGATCC TCGGTTCAAT 
GCAGAAGTTG ACCAAATTAC AGGTTACAAG ACACAGAGTA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAATGGT 
GGGACATTCA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT 
GTTCTTCATA ATGCTCAACT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGCTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTCTAAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAGGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGC GTCTTTCACA TGGAATGTGA GGAATTAGAA 
AAATTGCCAG ATACGTTAAC AAGGGAACGT GATGCAAACA GAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGACAAAAGA 
TTTCCCTGGA CAAATGAAAA CACAGGAAAT GTAAACCAGC AATGCATTAG AAGTTTGCTT 
TGTACTCCTA TAAAAAATGG AAAGAAGAAT AAAGTGATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGATGA ACAGTTTCTG 
GAGGCTTTTG TCATCTTTTG CGGCTTGGGG ATCCAGAACA CACAGATGTA TGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA GATGGTCACA CTGGAGGTTC TATCCTACCA TGCTTCAGCA 
GCAGAGGAGG AAACAAAAGA GCTCCAATCC TTGGCGGCTG CTGTGGTACC ATCTGCTCAG 
ACGCTTAAAA TTACTGACTT CAGCTTCAGT GACTTTGAGC TGTCTGACCT AGAAACAGCA 
CTGTGCACAA TTCGGATGTT CACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTTCTTT GCAGGTGGAT TTTAAGTGTT AAGAAGAATT ACAGGAAGAA TGTTGCCTAT 
CATAATTGGA GACACGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTTT AAAGGCCGGC 
AAGATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCCC TGCTGATTGC TGCTCTCAGC 
CACGACTTGG ACCACCGTGG TGTGAATAAC TCTTATATAC AGCGAAGTGA ACACCCACTT 
GCTCAACTCT ACTGCCATTC AATCATGGAG CATCATCATT TTGACCAGTG CCTGATGATT 
CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG 
CTGAAAATAA TCAAGCAAGC TATTTTAGCC ACAGACCTAG CATTGTACAT TAAGAGACGA 
GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAACT TGGAAGATCC TCATCAAAAA 
GAGTTGTTTT TAGCGATGCT GATGACAGCT TGTGACCTGT CTGCCATTAC AAAACCGTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGACCA AGGAGACAGA 
GAGAGAAAAG AACTCAACAT AGAGCCTGCT GATCTAATGA ACAGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTATA TGAGGCTCTG 
ACCCACGTAT CAGAGGACTG CTTCCCTTTG CTGGATGGCT GCAGAAAGAA CAGGCAAAAA 
TGGCAGGCCC TAGCAGAACA GCAGGAGAAA ACGCTGATTA ATGGGGAAAG CAGCCAGGCC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639102.1
CDS	108..2579
Translation	MVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	XM_005639045.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639045.2

ORF Insert Sequence:

ATGGTCAATG CCTGGTTCGC TGAGAGAGTC CACACCATCC CTGTGTGTAA AGAAGGCATC 
AGAGGCCACG CAGAATCTTG CTCTTGCTCC TCGCAGCAGA GTTCCCGTGC AGACAGCAGT 
GCGCCTGGCA CACCAACCAG GAAAATCTCT GCCTCTGAAT TTGACCGGCC TCTTAGACCC 
ATTGTCGTCA AAGATTCTGA GGGTACTGTG AGCTTCCTCG CTGACTCAGA AAAGAAGGAA 
CAGATGCCTC TAACCCCCCC AAGGTTTGAT AATGATGAAG GGGACCAGTG CTCGAGACTC 
TTGGAATTAG TGAAAGATAT TTCTAGTCAT TTGGATGTCA CAGCCTTATG TCATAAAATT 
TTCTTGCATA TTCATGGACT TATCTCCGCT GATCGCTATT CCCTGTTTCT TGTCTGTGAG 
GACAGCTCCA ATGACAAGTT TCTTATCAGC CGCCTGTTTG ATGTTGCAGA AGGTTCAACA 
CTGGAAGAAG CTTCAAATAA CTGTATCCGC TTAGAATGGA ACAAAGGCAT TGTGGGACAT 
GTGGCAGCAC TTGGTGAGCC CTTGAACATC AAAGATGCTT ATGAGGATCC TCGGTTCAAT 
GCAGAAGTTG ACCAAATTAC AGGTTACAAG ACACAGAGTA TTCTTTGTAT GCCAATTAAG 
AATCATAGGG AAGAGGTTGT TGGTGTAGCC CAGGCCATCA ACAAGAAATC AGGAAATGGT 
GGGACATTCA CTGAAAAAGA TGAAAAGGAC TTTGCTGCTT ATTTGGCATT TTGTGGTATT 
GTTCTTCATA ATGCTCAACT CTATGAGACT TCACTGCTGG AGAACAAGAG AAATCAGGTG 
CTGCTTGACC TTGCTAGCTT AATTTTTGAA GAACAACAAT CATTAGAAGT AATTCTAAAG 
AAAATAGCTG CCACTATTAT CTCTTTCATG CAGGTGCAGA AATGCACCAT TTTCATAGTG 
GATGAAGATT GCTCCGATTC TTTTTCTAGC GTCTTTCACA TGGAATGTGA GGAATTAGAA 
AAATTGCCAG ATACGTTAAC AAGGGAACGT GATGCAAACA GAATCAATTA CATGTATGCT 
CAGTATGTCA AAAATACTAT GGAACCACTT AATATCCCAG ATGTCAGTAA GGACAAAAGA 
TTTCCCTGGA CAAATGAAAA CACAGGAAAT GTAAACCAGC AATGCATTAG AAGTTTGCTT 
TGTACTCCTA TAAAAAATGG AAAGAAGAAT AAAGTGATAG GGGTTTGCCA ACTTGTTAAT 
AAGATGGAGG AGAATACTGG CAAGGTTAAG CCTTTCAACC GAAATGATGA ACAGTTTCTG 
GAGGCTTTTG TCATCTTTTG CGGCTTGGGG ATCCAGAACA CACAGATGTA TGAAGCAGTG 
GAGAGAGCCA TGGCCAAGCA GATGGTCACA CTGGAGGTTC TATCCTACCA TGCTTCAGCA 
GCAGAGGAGG AAACAAAAGA GCTCCAATCC TTGGCGGCTG CTGTGGTACC ATCTGCTCAG 
ACGCTTAAAA TTACTGACTT CAGCTTCAGT GACTTTGAGC TGTCTGACCT AGAAACAGCA 
CTGTGCACAA TTCGGATGTT CACTGACCTC AACCTTGTGC AGAACTTCCA GATGAAACAT 
GAGGTTCTTT GCAGGTGGAT TTTAAGTGTT AAGAAGAATT ACAGGAAGAA TGTTGCCTAT 
CATAATTGGA GACACGCCTT TAATACAGCT CAGTGCATGT TTGCTGCTTT AAAGGCCGGC 
AAGATTCAGA ACAAGCTGAC TGACCTGGAG ATACTTGCCC TGCTGATTGC TGCTCTCAGC 
CACGACTTGG ACCACCGTGG TGTGAATAAC TCTTATATAC AGCGAAGTGA ACACCCACTT 
GCTCAACTCT ACTGCCATTC AATCATGGAG CATCATCATT TTGACCAGTG CCTGATGATT 
CTTAATAGTC CAGGCAATCA GATTCTCAGT GGCCTCTCCA TTGAAGAATA TAAGACCACG 
CTGAAAATAA TCAAGCAAGC TATTTTAGCC ACAGACCTAG CATTGTACAT TAAGAGACGA 
GGAGAATTTT TTGAACTTAT AAGAAAAAAT CAATTCAACT TGGAAGATCC TCATCAAAAA 
GAGTTGTTTT TAGCGATGCT GATGACAGCT TGTGACCTGT CTGCCATTAC AAAACCGTGG 
CCTATTCAAC AACGGATAGC AGAACTTGTA GCAACTGAAT TTTTTGACCA AGGAGACAGA 
GAGAGAAAAG AACTCAACAT AGAGCCTGCT GATCTAATGA ACAGGGAGAA GAAAAACAAA 
ATCCCAAGTA TGCAAGTTGG GTTCATAGAT GCCATCTGCT TGCAACTATA TGAGGCTCTG 
ACCCACGTAT CAGAGGACTG CTTCCCTTTG CTGGATGGCT GCAGAAAGAA CAGGCAAAAA 
TGGCAGGCCC TAGCAGAACA GCAGGAGAAA ACGCTGATTA ATGGGGAAAG CAGCCAGGCC 
AAGCGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_022413385.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022269093.1 Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe284003
Clone ID Related Accession (Same CDS sequence)	XM_022413385.1
Accession Version	XM_022413385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGTTCTTTG CAGGTGGATT 
TTAAGTGTTA AGAAGAATTA CAGGAAGAAT GTTGCCTATC ATAATTGGAG ACACGCCTTT 
AATACAGCTC AGTGCATGTT TGCTGCTTTA AAGGCCGGCA AGATTCAGAA CAAGCTGACT 
GACCTGGAGA TACTTGCCCT GCTGATTGCT GCTCTCAGCC ACGACTTGGA CCACCGTGGT 
GTGAATAACT CTTATATACA GCGAAGTGAA CACCCACTTG CTCAACTCTA CTGCCATTCA 
ATCATGGAGC ATCATCATTT TGACCAGTGC CTGATGATTC TTAATAGTCC AGGCAATCAG 
ATTCTCAGTG GCCTCTCCAT TGAAGAATAT AAGACCACGC TGAAAATAAT CAAGCAAGCT 
ATTTTAGCCA CAGACCTAGC ATTGTACATT AAGAGACGAG GAGAATTTTT TGAACTTATA 
AGAAAAAATC AATTCAACTT GGAAGATCCT CATCAAAAAG AGTTGTTTTT AGCGATGCTG 
ATGACAGCTT GTGACCTGTC TGCCATTACA AAACCGTGGC CTATTCAACA ACGGATAGCA 
GAACTTGTAG CAACTGAATT TTTTGACCAA GGAGACAGAG AGAGAAAAGA ACTCAACATA 
GAGCCTGCTG ATCTAATGAA CAGGGAGAAG AAAAACAAAA TCCCAAGTAT GCAAGTTGGG 
TTCATAGATG CCATCTGCTT GCAACTATAT GAGGCTCTGA CCCACGTATC AGAGGACTGC 
TTCCCTTTGC TGGATGGCTG CAGAAAGAAC AGGCAAAAAT GGCAGGCCCT AGCAGAACAG 
CAGGAGAAAA CGCTGATTAA TGGGGAAAGC AGCCAGGCCA AGCGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269093.1
CDS	53..2503
Translation	MERGSPGAGAARLPRDQDSVEAWLDDHRDFTFSYFVKKATREMVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	XM_022413385.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413385.1

ORF Insert Sequence:

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGTTCTTTG CAGGTGGATT 
TTAAGTGTTA AGAAGAATTA CAGGAAGAAT GTTGCCTATC ATAATTGGAG ACACGCCTTT 
AATACAGCTC AGTGCATGTT TGCTGCTTTA AAGGCCGGCA AGATTCAGAA CAAGCTGACT 
GACCTGGAGA TACTTGCCCT GCTGATTGCT GCTCTCAGCC ACGACTTGGA CCACCGTGGT 
GTGAATAACT CTTATATACA GCGAAGTGAA CACCCACTTG CTCAACTCTA CTGCCATTCA 
ATCATGGAGC ATCATCATTT TGACCAGTGC CTGATGATTC TTAATAGTCC AGGCAATCAG 
ATTCTCAGTG GCCTCTCCAT TGAAGAATAT AAGACCACGC TGAAAATAAT CAAGCAAGCT 
ATTTTAGCCA CAGACCTAGC ATTGTACATT AAGAGACGAG GAGAATTTTT TGAACTTATA 
AGAAAAAATC AATTCAACTT GGAAGATCCT CATCAAAAAG AGTTGTTTTT AGCGATGCTG 
ATGACAGCTT GTGACCTGTC TGCCATTACA AAACCGTGGC CTATTCAACA ACGGATAGCA 
GAACTTGTAG CAACTGAATT TTTTGACCAA GGAGACAGAG AGAGAAAAGA ACTCAACATA 
GAGCCTGCTG ATCTAATGAA CAGGGAGAAG AAAAACAAAA TCCCAAGTAT GCAAGTTGGG 
TTCATAGATG CCATCTGCTT GCAACTATAT GAGGCTCTGA CCCACGTATC AGAGGACTGC 
TTCCCTTTGC TGGATGGCTG CAGAAAGAAC AGGCAAAAAT GGCAGGCCCT AGCAGAACAG 
CAGGAGAAAA CGCTGATTAA TGGGGAAAGC AGCCAGGCCA AGCGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_005639043.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005639100.1 Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe29595
Clone ID Related Accession (Same CDS sequence)	XM_005639043.2 , XM_005639043.3
Accession Version	XM_005639043.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GATAGCAGAA CTTGTAGCAA CTGAATTTTT TGACCAAGGA 
GACAGAGAGA GAAAAGAACT CAACATAGAG CCTGCTGATC TAATGAACAG GGAGAAGAAA 
AACAAAATCC CAAGTATGCA AGTTGGGTTC ATAGATGCCA TCTGCTTGCA ACTATATGAG 
GCTCTGACCC ACGTATCAGA GGACTGCTTC CCTTTGCTGG ATGGCTGCAG AAAGAACAGG 
CAAAAATGGC AGGCCCTAGC AGAACAGCAG GAGAAAACGC TGATTAATGG GGAAAGCAGC 
CAGGCCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639100.1
CDS	307..2904
Translation	MERGSPGAGAARLPRDQDSVEAWLDDHRDFTFSYFVKKATREMVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	XM_005639043.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639043.2

ORF Insert Sequence:

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GATAGCAGAA CTTGTAGCAA CTGAATTTTT TGACCAAGGA 
GACAGAGAGA GAAAAGAACT CAACATAGAG CCTGCTGATC TAATGAACAG GGAGAAGAAA 
AACAAAATCC CAAGTATGCA AGTTGGGTTC ATAGATGCCA TCTGCTTGCA ACTATATGAG 
GCTCTGACCC ACGTATCAGA GGACTGCTTC CCTTTGCTGG ATGGCTGCAG AAAGAACAGG 
CAAAAATGGC AGGCCCTAGC AGAACAGCAG GAGAAAACGC TGATTAATGG GGAAAGCAGC 
CAGGCCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_005639043.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005639100.1 Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe29595
Clone ID Related Accession (Same CDS sequence)	XM_005639043.2 , XM_005639043.3
Accession Version	XM_005639043.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GATAGCAGAA CTTGTAGCAA CTGAATTTTT TGACCAAGGA 
GACAGAGAGA GAAAAGAACT CAACATAGAG CCTGCTGATC TAATGAACAG GGAGAAGAAA 
AACAAAATCC CAAGTATGCA AGTTGGGTTC ATAGATGCCA TCTGCTTGCA ACTATATGAG 
GCTCTGACCC ACGTATCAGA GGACTGCTTC CCTTTGCTGG ATGGCTGCAG AAAGAACAGG 
CAAAAATGGC AGGCCCTAGC AGAACAGCAG GAGAAAACGC TGATTAATGG GGAAAGCAGC 
CAGGCCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639100.1
CDS	283..2880
Translation	MERGSPGAGAARLPRDQDSVEAWLDDHRDFTFSYFVKKATREMVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	XM_005639043.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639043.3

ORF Insert Sequence:

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GATAGCAGAA CTTGTAGCAA CTGAATTTTT TGACCAAGGA 
GACAGAGAGA GAAAAGAACT CAACATAGAG CCTGCTGATC TAATGAACAG GGAGAAGAAA 
AACAAAATCC CAAGTATGCA AGTTGGGTTC ATAGATGCCA TCTGCTTGCA ACTATATGAG 
GCTCTGACCC ACGTATCAGA GGACTGCTTC CCTTTGCTGG ATGGCTGCAG AAAGAACAGG 
CAAAAATGGC AGGCCCTAGC AGAACAGCAG GAGAAAACGC TGATTAATGG GGAAAGCAGC 
CAGGCCAAGC GGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_005639044.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005639101.1 Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe29596
Clone ID Related Accession (Same CDS sequence)	XM_005639044.1
Accession Version	XM_005639044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GATAGCAGAA CTTGTAGCAA CTGAATTTTT TGACCAAGGA 
GACAGAGAGA GAAAAGAACT CAACATAGAG CCTGCTGCTC TGACCCACGT ATCAGAGGAC 
TGCTTCCCTT TGCTGGATGG CTGCAGAAAG AACAGGCAAA AATGGCAGGC CCTAGCAGAA 
CAGCAGGAGA AAACGCTGAT TAATGGGGAA AGCAGCCAGG CCAAGCGGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005639101.1
CDS	109..2622
Translation	MERGSPGAGAARLPRDQDSVEAWLDDHRDFTFSYFVKKATREMVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPAALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	XM_005639044.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005639044.1

ORF Insert Sequence:

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GATAGCAGAA CTTGTAGCAA CTGAATTTTT TGACCAAGGA 
GACAGAGAGA GAAAAGAACT CAACATAGAG CCTGCTGCTC TGACCCACGT ATCAGAGGAC 
TGCTTCCCTT TGCTGGATGG CTGCAGAAAG AACAGGCAAA AATGGCAGGC CCTAGCAGAA 
CAGCAGGAGA AAACGCTGAT TAATGGGGAA AGCAGCCAGG CCAAGCGGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_014109885.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965360.1 Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe44532
Clone ID Related Accession (Same CDS sequence)	XM_014109885.1 , XM_014109885.2
Accession Version	XM_014109885.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GGCTCTGACC CACGTATCAG AGGACTGCTT CCCTTTGCTG 
GATGGCTGCA GAAAGAACAG GCAAAAATGG CAGGCCCTAG CAGAACAGCA GGAGAAAACG 
CTGATTAATG GGGAAAGCAG CCAGGCCAAG CGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965360.1
CDS	109..2547
Translation	MERGSPGAGAARLPRDQDSVEAWLDDHRDFTFSYFVKKATREMVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	XM_014109885.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109885.1

ORF Insert Sequence:

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GGCTCTGACC CACGTATCAG AGGACTGCTT CCCTTTGCTG 
GATGGCTGCA GAAAGAACAG GCAAAAATGG CAGGCCCTAG CAGAACAGCA GGAGAAAACG 
CTGATTAATG GGGAAAGCAG CCAGGCCAAG CGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_014109885.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965360.1 Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X4, mRNA.

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CloneID	OCe44532
Clone ID Related Accession (Same CDS sequence)	XM_014109885.1 , XM_014109885.2
Accession Version	XM_014109885.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GGCTCTGACC CACGTATCAG AGGACTGCTT CCCTTTGCTG 
GATGGCTGCA GAAAGAACAG GCAAAAATGG CAGGCCCTAG CAGAACAGCA GGAGAAAACG 
CTGATTAATG GGGAAAGCAG CCAGGCCAAG CGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965360.1
CDS	53..2491
Translation	MERGSPGAGAARLPRDQDSVEAWLDDHRDFTFSYFVKKATREMVNAWFAERVHTIPVCKEGIRGHAESCSCSSQQSSRADSSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLADSEKKEQMPLTPPRFDNDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKLPDTLTRERDANRINYMYAQYVKNTMEPLNIPDVSKDKRFPWTNENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETKELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKTLINGESSQAKRN

Target ORF information:

RefSeq Version	XM_014109885.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphodiesterase 5A (PDE5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109885.2

ORF Insert Sequence:

ATGGAGCGGG GCAGCCCCGG CGCCGGGGCG GCGCGGCTGC CGCGGGACCA GGACTCCGTG 
GAAGCGTGGC TGGATGATCA CCGGGACTTC ACCTTCTCTT ACTTTGTTAA GAAAGCCACC 
AGAGAAATGG TCAATGCCTG GTTCGCTGAG AGAGTCCACA CCATCCCTGT GTGTAAAGAA 
GGCATCAGAG GCCACGCAGA ATCTTGCTCT TGCTCCTCGC AGCAGAGTTC CCGTGCAGAC 
AGCAGTGCGC CTGGCACACC AACCAGGAAA ATCTCTGCCT CTGAATTTGA CCGGCCTCTT 
AGACCCATTG TCGTCAAAGA TTCTGAGGGT ACTGTGAGCT TCCTCGCTGA CTCAGAAAAG 
AAGGAACAGA TGCCTCTAAC CCCCCCAAGG TTTGATAATG ATGAAGGGGA CCAGTGCTCG 
AGACTCTTGG AATTAGTGAA AGATATTTCT AGTCATTTGG ATGTCACAGC CTTATGTCAT 
AAAATTTTCT TGCATATTCA TGGACTTATC TCCGCTGATC GCTATTCCCT GTTTCTTGTC 
TGTGAGGACA GCTCCAATGA CAAGTTTCTT ATCAGCCGCC TGTTTGATGT TGCAGAAGGT 
TCAACACTGG AAGAAGCTTC AAATAACTGT ATCCGCTTAG AATGGAACAA AGGCATTGTG 
GGACATGTGG CAGCACTTGG TGAGCCCTTG AACATCAAAG ATGCTTATGA GGATCCTCGG 
TTCAATGCAG AAGTTGACCA AATTACAGGT TACAAGACAC AGAGTATTCT TTGTATGCCA 
ATTAAGAATC ATAGGGAAGA GGTTGTTGGT GTAGCCCAGG CCATCAACAA GAAATCAGGA 
AATGGTGGGA CATTCACTGA AAAAGATGAA AAGGACTTTG CTGCTTATTT GGCATTTTGT 
GGTATTGTTC TTCATAATGC TCAACTCTAT GAGACTTCAC TGCTGGAGAA CAAGAGAAAT 
CAGGTGCTGC TTGACCTTGC TAGCTTAATT TTTGAAGAAC AACAATCATT AGAAGTAATT 
CTAAAGAAAA TAGCTGCCAC TATTATCTCT TTCATGCAGG TGCAGAAATG CACCATTTTC 
ATAGTGGATG AAGATTGCTC CGATTCTTTT TCTAGCGTCT TTCACATGGA ATGTGAGGAA 
TTAGAAAAAT TGCCAGATAC GTTAACAAGG GAACGTGATG CAAACAGAAT CAATTACATG 
TATGCTCAGT ATGTCAAAAA TACTATGGAA CCACTTAATA TCCCAGATGT CAGTAAGGAC 
AAAAGATTTC CCTGGACAAA TGAAAACACA GGAAATGTAA ACCAGCAATG CATTAGAAGT 
TTGCTTTGTA CTCCTATAAA AAATGGAAAG AAGAATAAAG TGATAGGGGT TTGCCAACTT 
GTTAATAAGA TGGAGGAGAA TACTGGCAAG GTTAAGCCTT TCAACCGAAA TGATGAACAG 
TTTCTGGAGG CTTTTGTCAT CTTTTGCGGC TTGGGGATCC AGAACACACA GATGTATGAA 
GCAGTGGAGA GAGCCATGGC CAAGCAGATG GTCACACTGG AGGTTCTATC CTACCATGCT 
TCAGCAGCAG AGGAGGAAAC AAAAGAGCTC CAATCCTTGG CGGCTGCTGT GGTACCATCT 
GCTCAGACGC TTAAAATTAC TGACTTCAGC TTCAGTGACT TTGAGCTGTC TGACCTAGAA 
ACAGCACTGT GCACAATTCG GATGTTCACT GACCTCAACC TTGTGCAGAA CTTCCAGATG 
AAACATGAGG TTCTTTGCAG GTGGATTTTA AGTGTTAAGA AGAATTACAG GAAGAATGTT 
GCCTATCATA ATTGGAGACA CGCCTTTAAT ACAGCTCAGT GCATGTTTGC TGCTTTAAAG 
GCCGGCAAGA TTCAGAACAA GCTGACTGAC CTGGAGATAC TTGCCCTGCT GATTGCTGCT 
CTCAGCCACG ACTTGGACCA CCGTGGTGTG AATAACTCTT ATATACAGCG AAGTGAACAC 
CCACTTGCTC AACTCTACTG CCATTCAATC ATGGAGCATC ATCATTTTGA CCAGTGCCTG 
ATGATTCTTA ATAGTCCAGG CAATCAGATT CTCAGTGGCC TCTCCATTGA AGAATATAAG 
ACCACGCTGA AAATAATCAA GCAAGCTATT TTAGCCACAG ACCTAGCATT GTACATTAAG 
AGACGAGGAG AATTTTTTGA ACTTATAAGA AAAAATCAAT TCAACTTGGA AGATCCTCAT 
CAAAAAGAGT TGTTTTTAGC GATGCTGATG ACAGCTTGTG ACCTGTCTGC CATTACAAAA 
CCGTGGCCTA TTCAACAACG GGCTCTGACC CACGTATCAG AGGACTGCTT CCCTTTGCTG 
GATGGCTGCA GAAAGAACAG GCAAAAATGG CAGGCCCTAG CAGAACAGCA GGAGAAAACG 
CTGATTAATG GGGAAAGCAG CCAGGCCAAG CGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PDE5A
Entrez Gene ID	403825
Full Name	phosphodiesterase 5A
Synonyms	PDE5
General protein information	Preferred Names phosphodiesterase 5A Names cGMP-specific 3',5'-cyclic phosphodiesterase 3',5'-Cyclic GMP Phosphodiesterase CGB-PDE cGMP-binding cGMP-specific phosphodiesterase
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Danio rerio (zebrafish)	pde5ab	NP_001116732.1
Drosophila melanogaster (fruit fly)	Pde11	NP_001188837.1
Pan troglodytes (chimpanzee)	PDE5A	XP_001150967.2
	PDE5A	XP_001099652.1
Canis lupus familiaris (dog)	PDE5A	NP_001003188.1
Bos taurus (cattle)	PDE5A	NP_776842.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008646	XP_314743.3
Rattus norvegicus (Norway rat)	Pde5a	NP_598268.1
Xenopus tropicalis (tropical clawed frog)	pde5a	NP_001072812.1
Homo sapiens (human)	PDE5A	NP_001074.2
Mus musculus (house mouse)	Pde5a	NP_700471.2
Gallus gallus (chicken)	PDE5A	XP_420631.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PDE5A cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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