LOC104745780 cDNA ORF clone, Camelina sativa(false flax)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104745780
Entrez Gene ID	104745780
Full Name	increased DNA methylation 1-like
Gene Type	protein-coding
Organism	Camelina sativa(false flax)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010467131.2	XP_010465433.1	increased DNA methylation 1-like isoform X2
XM_010467129.2	XP_010465431.1	increased DNA methylation 1-like isoform X1
XM_010467130.2	XP_010465432.1	increased DNA methylation 1-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC104745780 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104745780 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa189412	XM_010467130.2 Latest version!	Camelina sativa increased DNA methylation 1-like (LOC104745780), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa189412	XM_010467129.2 Latest version!	Camelina sativa increased DNA methylation 1-like (LOC104745780), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa189413	XM_010467131.2 Latest version!	Camelina sativa increased DNA methylation 1-like (LOC104745780), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104745780 ( XM_010467129.2 ) cDNA ORF clone, Camelina sativa(false flax) -> XP_010465431.1 Camelina sativa increased DNA methylation 1-like (LOC104745780), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa189412
Clone ID Related Accession (Same CDS sequence)	XM_010467129.2 , XM_010467130.2
Accession Version	XM_010467129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Camelina sativa(false flax)
Product	increased DNA methylation 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_025699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010467129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Camelina sativa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCGCA AAGCTGAGAT TGAGATGTTA GGAGGTGACG ACTGCTTTGA AGGGTCTTCT 
GAGGATCATC AGATATTCAG GCAAGTTTTC TTTGGAAGTG ATTCTGGAAA TACTACTAAA 
AGATGTCTCG TTACTGGAGT CATTAACTTC GAATCTGATT CCACTAAGAA TGTTAATTCC 
TCTCTGAGTT CCAACAATGA CAACTCTGTG GTAACAAGTG GGTATGAGGC ATCTGCTTCA 
AAAGATGGTT CAGACTTTAC CACGAAAGCT AAGAGACTGA AGCTTTCAGG TAATAAGCAT 
TTGGATGCAA GGGACGTAAA GGGATCCGCT TTCAGTGGTT TCCCAAATTC GGATATAGCT 
AGAGAAACAA TTCCTCTCCA TTTAGTTGAA TCGTCTAACA AGGGTGTTTC AACTAGCTCT 
TATTTGCTAA AGCAAAGCAT AGAAAAGGGG AAAGAAGTTT ATCTGGGTGG CATTCTCACA 
GAAAAGTGCA AGTCACAAAA CTTGGACAGA TCCGATGGAA AGGAATTTAA AGCAATTGCC 
TCTCCTGTTT CTCAGGAGAG CTTCGCAACC AGAATGATCT GTGCAGGTGC TTCTACGCCT 
CATAGTGAGA AGGCTTGCTT CTCACCGCAG TTGAACAACG GGTCTAAAGT ATCTCCTAAT 
GAGCTAATTA TGTCTAAAAC CTGTCTAAAG ATTGATCCTA AAGATGATCC CCGTCCTCTT 
ATCTACAAAT ATGTCTGTAA GTTGCTAAAT GCTGCCAGAT GGAAAATCGA GAAGCGCGGA 
AGATCAGGTA GGAAATACTG TGATACATTT TACATATCGC CTGAGGGACG GAAATTTCGT 
GAATTTCCGT CAGCTTGGAA ATCGTTGGGT GGGATCCTAT TAGCTGGTCA TAAGTTAATG 
GATACCAGTA CTAAGAAATG GACCGGTATT AATGACTTCT GGTCTGATCT CTCTCTTACA 
CTGTTGGACA TGGAGGAGAA CATGAAAAAT CTAAATCTTG CCAACACACG GGCACTTTGG 
TGGAGTGCCT TGGAGCCTTT TGTGATTGCT GTCTTCATTT CTAAGCAAGT TGGATCTTTA 
AGAAGAGGAA ATAAAGTCGA AGTTGCAAGG ATTTCAATAG TTGACAAGTT GAAGAAAGAA 
GATGCGATTT GTTTGAACAT GATTTCAGGG TGTCCTGAAA GTGTGCTGAC AGTATCTGAA 
GGCAGTCATC TTGTTAATGA CGTGGATGCT ATCCAAGAGA TTCATTCAGA TCTTGAGGTG 
CAAACTAAAA TTGTCCCTCG AAAAGTTTCT TCAAGACTAG AGAGGCAGAA TATAGTTGGT 
AAGGAAATCT ATGGGACTGA TGAGCAGGAG GTTAGCAAGG GCGTTGTAGC AGAAGACATG 
CATGAAAGTG TTATGAGAAA GAAGTTACAC AGAAGATCAA AAAAAGTATC GGATATCAAA 
CCAGTTTCCT TGGACCAACA TGATTCACTA GATTCTAATT CTCTCGACAG CCTTGAATGC 
CAGGATAAGG AGATGAGCAA CATTCACCTC ATCGCAAAGG GAAGTCCGGA TGAAAGGTTG 
AGCAATGAGA AGATTAAGAA GAATCCATGT TGCAATTTAA AGAAAGGAAG AAAGAAAGCT 
AGGAAGCACT ATTCTCAGGA TGATGATCTT ATGGCGTCGA CAATAATCAG AAACAAAGTT 
AAGGTTTGCA GATCAAGCCA GAAAAAGAAA ACCCAGAAAC CAAAGGCTAG AACCAAAAAA 
AGAAACAATA GGGGAGGGTG CAGGCTGCTT CCCCGAAGCA CAAGCAATGT GGAAAATCAA 
TTATGTCAAG GAAATTGGTC TAATTTGGGT CCAAGAACTG TCTTATCATG GTTGATAGCT 
ACCAAAGTAA TTTCCAGGGA CGAAGTAATC CAGTTACGAG ATCCAGATGA TGATACTGTG 
GTAAAAACAG GCCTCGTAAC TAAAGATGGA GTGGTTTGTA CATGTTGCAA CAGGACTGTA 
TCACTTTCTG AATTCAAGAA CCATGCTGGA TTTAACCAGA ATTGTCCATG TCTGAATCTT 
TTCATGGGGT CAGGAAAGCC TTTAGCTTCA TGTCAGCTGG AAGCTTGGTC TGCTGAGTAT 
AAGGCGCGGA GAAATGGATG GAAATCAGTG GAAGCTTCTG ATGATGATCC AAATGATGAT 
TCTTGTGGAG TTTGTGGTGA TGGGGGTGAA CTGATCTGCT GTGATAATTG TCCGTCGACC 
TTCCATCAGG CTTGCTTATC AATGCAGGTG CTCCCTGAAG GAAGTTGGTA TTGCTCGAGC 
TGCACATGTT GGATATGCAG CGAGTTGGTT AGTGATAATG CTGAACGCTA TCAGGATTTT 
AAATGTTCTC ATTGTGCGCA TAAATATCAT GGAATATGTT TGCAAGGGGA AAGTAAACCG 
AGAAAACTTT CTCCAGAGAC CTACTTTTGT GGTAAAAATT GCGAGAAGGT GTACACTGGT 
CTCAGTTCTC GTGTTGGGGT CATTAACCCT AATGCTGCTG GTCTGTCTTG GTCAATTCTG 
AAATGTTTTC AAGAAGATGG AAATGTTCAT TCTGCCCGAA GGTTGGCGCT GAAGGCTGAA 
TGCAACTCGA AATTAGCTGT TGCCCTATCA ATTATGGAGG AAAGCTTTCA GTCTATGATA 
GATCCAAGAA CTGGTATTGA CATGATACCT CATGTGCTAT ACAACTGGGG GTCAAATTTT 
GCTCGTTTAG ACTTTGATGG GTTTTACACT GTGGTTGTGG AGAAGGACGA TGTGATGATC 
TCAGTAGCAT CTATCAGGGT GCATGGAGTA ACTATTGCAG AGATGCCTCT TGCAGCTACC 
TGCAGCAAAT ATCGCCGACA GGGGATGTGC AGAATCCTCG TGGCTGCCAT CGAAGAGCTG 
CTTATGTCTC TCAAAGTGGA GAAGCTTGTG GTAGCTGCAT TGCCGAGTTT AGTGGAAACT 
TGGACTGAAG GTTTTGGCTT CAAACCCATG GATGATGAAG AACGTGATGC TCTCAAAAGA 
ATAAACTTGA TGGTCTTCCC TGGGACAATT CTGCTCAAGA AAACGCTATA CGAGAGCACA 
AAAACCAACA TTGTGAACGA CGTTTGTCTT TCTAAAATTC AAAGTGAACC ATCAAACAAA 
GAAGCAGACC AAGAGCCAGG CCTAGACAAA GCCGGGTTTC CCATACATAC CAAAGTTGAG 
AGCTGTGATC AGATGGTTCC AGCAGGTTCA GATGAAGACC CGGCACCAGG CTTCCCCGTG 
CCACTTGGAG CCAAGGAAAC AGAACTAACT TCCGAAACAG AGAACCCAAG CCGAGACAGC 
AATGCCGATG ATAGGCCAAA AAAAACAAGA GTATCCATGG GAGAAAAAGA AGAAGAAGAA 
GGTTTGCAGG AAGAGGTATC AAAGATTTCA GAAGAAGGAA AAGAAATGAC AAAAGCTTCT 
TCTTCCTCTG CAGCTTTAGA AGAAGTAAGT GATGTAGGAG TAGTAGTAGT AAATAATGTT 
AGTGATGACA TGTTGCTTTG TTTTGATGAA CAGCTTGACT CTGATAGCTC TGAAGAAGAA 
AACTCGGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010465431.1
CDS	540..4091
Translation	MFRKAEIEMLGGDDCFEGSSEDHQIFRQVFFGSDSGNTTKRCLVTGVINFESDSTKNVNSSLSSNNDNSVVTSGYEASASKDGSDFTTKAKRLKLSGNKHLDARDVKGSAFSGFPNSDIARETIPLHLVESSNKGVSTSSYLLKQSIEKGKEVYLGGILTEKCKSQNLDRSDGKEFKAIASPVSQESFATRMICAGASTPHSEKACFSPQLNNGSKVSPNELIMSKTCLKIDPKDDPRPLIYKYVCKLLNAARWKIEKRGRSGRKYCDTFYISPEGRKFREFPSAWKSLGGILLAGHKLMDTSTKKWTGINDFWSDLSLTLLDMEENMKNLNLANTRALWWSALEPFVIAVFISKQVGSLRRGNKVEVARISIVDKLKKEDAICLNMISGCPESVLTVSEGSHLVNDVDAIQEIHSDLEVQTKIVPRKVSSRLERQNIVGKEIYGTDEQEVSKGVVAEDMHESVMRKKLHRRSKKVSDIKPVSLDQHDSLDSNSLDSLECQDKEMSNIHLIAKGSPDERLSNEKIKKNPCCNLKKGRKKARKHYSQDDDLMASTIIRNKVKVCRSSQKKKTQKPKARTKKRNNRGGCRLLPRSTSNVENQLCQGNWSNLGPRTVLSWLIATKVISRDEVIQLRDPDDDTVVKTGLVTKDGVVCTCCNRTVSLSEFKNHAGFNQNCPCLNLFMGSGKPLASCQLEAWSAEYKARRNGWKSVEASDDDPNDDSCGVCGDGGELICCDNCPSTFHQACLSMQVLPEGSWYCSSCTCWICSELVSDNAERYQDFKCSHCAHKYHGICLQGESKPRKLSPETYFCGKNCEKVYTGLSSRVGVINPNAAGLSWSILKCFQEDGNVHSARRLALKAECNSKLAVALSIMEESFQSMIDPRTGIDMIPHVLYNWGSNFARLDFDGFYTVVVEKDDVMISVASIRVHGVTIAEMPLAATCSKYRRQGMCRILVAAIEELLMSLKVEKLVVAALPSLVETWTEGFGFKPMDDEERDALKRINLMVFPGTILLKKTLYESTKTNIVNDVCLSKIQSEPSNKEADQEPGLDKAGFPIHTKVESCDQMVPAGSDEDPAPGFPVPLGAKETELTSETENPSRDSNADDRPKKTRVSMGEKEEEEGLQEEVSKISEEGKEMTKASSSSAALEEVSDVGVVVVNNVSDDMLLCFDEQLDSDSSEEENSE

Target ORF information:

RefSeq Version	XM_010467129.2
Organism	Camelina sativa(false flax)
Definition	Camelina sativa increased DNA methylation 1-like (LOC104745780), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010467129.2

ORF Insert Sequence:

ATGTTTCGCA AAGCTGAGAT TGAGATGTTA GGAGGTGACG ACTGCTTTGA AGGGTCTTCT 
GAGGATCATC AGATATTCAG GCAAGTTTTC TTTGGAAGTG ATTCTGGAAA TACTACTAAA 
AGATGTCTCG TTACTGGAGT CATTAACTTC GAATCTGATT CCACTAAGAA TGTTAATTCC 
TCTCTGAGTT CCAACAATGA CAACTCTGTG GTAACAAGTG GGTATGAGGC ATCTGCTTCA 
AAAGATGGTT CAGACTTTAC CACGAAAGCT AAGAGACTGA AGCTTTCAGG TAATAAGCAT 
TTGGATGCAA GGGACGTAAA GGGATCCGCT TTCAGTGGTT TCCCAAATTC GGATATAGCT 
AGAGAAACAA TTCCTCTCCA TTTAGTTGAA TCGTCTAACA AGGGTGTTTC AACTAGCTCT 
TATTTGCTAA AGCAAAGCAT AGAAAAGGGG AAAGAAGTTT ATCTGGGTGG CATTCTCACA 
GAAAAGTGCA AGTCACAAAA CTTGGACAGA TCCGATGGAA AGGAATTTAA AGCAATTGCC 
TCTCCTGTTT CTCAGGAGAG CTTCGCAACC AGAATGATCT GTGCAGGTGC TTCTACGCCT 
CATAGTGAGA AGGCTTGCTT CTCACCGCAG TTGAACAACG GGTCTAAAGT ATCTCCTAAT 
GAGCTAATTA TGTCTAAAAC CTGTCTAAAG ATTGATCCTA AAGATGATCC CCGTCCTCTT 
ATCTACAAAT ATGTCTGTAA GTTGCTAAAT GCTGCCAGAT GGAAAATCGA GAAGCGCGGA 
AGATCAGGTA GGAAATACTG TGATACATTT TACATATCGC CTGAGGGACG GAAATTTCGT 
GAATTTCCGT CAGCTTGGAA ATCGTTGGGT GGGATCCTAT TAGCTGGTCA TAAGTTAATG 
GATACCAGTA CTAAGAAATG GACCGGTATT AATGACTTCT GGTCTGATCT CTCTCTTACA 
CTGTTGGACA TGGAGGAGAA CATGAAAAAT CTAAATCTTG CCAACACACG GGCACTTTGG 
TGGAGTGCCT TGGAGCCTTT TGTGATTGCT GTCTTCATTT CTAAGCAAGT TGGATCTTTA 
AGAAGAGGAA ATAAAGTCGA AGTTGCAAGG ATTTCAATAG TTGACAAGTT GAAGAAAGAA 
GATGCGATTT GTTTGAACAT GATTTCAGGG TGTCCTGAAA GTGTGCTGAC AGTATCTGAA 
GGCAGTCATC TTGTTAATGA CGTGGATGCT ATCCAAGAGA TTCATTCAGA TCTTGAGGTG 
CAAACTAAAA TTGTCCCTCG AAAAGTTTCT TCAAGACTAG AGAGGCAGAA TATAGTTGGT 
AAGGAAATCT ATGGGACTGA TGAGCAGGAG GTTAGCAAGG GCGTTGTAGC AGAAGACATG 
CATGAAAGTG TTATGAGAAA GAAGTTACAC AGAAGATCAA AAAAAGTATC GGATATCAAA 
CCAGTTTCCT TGGACCAACA TGATTCACTA GATTCTAATT CTCTCGACAG CCTTGAATGC 
CAGGATAAGG AGATGAGCAA CATTCACCTC ATCGCAAAGG GAAGTCCGGA TGAAAGGTTG 
AGCAATGAGA AGATTAAGAA GAATCCATGT TGCAATTTAA AGAAAGGAAG AAAGAAAGCT 
AGGAAGCACT ATTCTCAGGA TGATGATCTT ATGGCGTCGA CAATAATCAG AAACAAAGTT 
AAGGTTTGCA GATCAAGCCA GAAAAAGAAA ACCCAGAAAC CAAAGGCTAG AACCAAAAAA 
AGAAACAATA GGGGAGGGTG CAGGCTGCTT CCCCGAAGCA CAAGCAATGT GGAAAATCAA 
TTATGTCAAG GAAATTGGTC TAATTTGGGT CCAAGAACTG TCTTATCATG GTTGATAGCT 
ACCAAAGTAA TTTCCAGGGA CGAAGTAATC CAGTTACGAG ATCCAGATGA TGATACTGTG 
GTAAAAACAG GCCTCGTAAC TAAAGATGGA GTGGTTTGTA CATGTTGCAA CAGGACTGTA 
TCACTTTCTG AATTCAAGAA CCATGCTGGA TTTAACCAGA ATTGTCCATG TCTGAATCTT 
TTCATGGGGT CAGGAAAGCC TTTAGCTTCA TGTCAGCTGG AAGCTTGGTC TGCTGAGTAT 
AAGGCGCGGA GAAATGGATG GAAATCAGTG GAAGCTTCTG ATGATGATCC AAATGATGAT 
TCTTGTGGAG TTTGTGGTGA TGGGGGTGAA CTGATCTGCT GTGATAATTG TCCGTCGACC 
TTCCATCAGG CTTGCTTATC AATGCAGGTG CTCCCTGAAG GAAGTTGGTA TTGCTCGAGC 
TGCACATGTT GGATATGCAG CGAGTTGGTT AGTGATAATG CTGAACGCTA TCAGGATTTT 
AAATGTTCTC ATTGTGCGCA TAAATATCAT GGAATATGTT TGCAAGGGGA AAGTAAACCG 
AGAAAACTTT CTCCAGAGAC CTACTTTTGT GGTAAAAATT GCGAGAAGGT GTACACTGGT 
CTCAGTTCTC GTGTTGGGGT CATTAACCCT AATGCTGCTG GTCTGTCTTG GTCAATTCTG 
AAATGTTTTC AAGAAGATGG AAATGTTCAT TCTGCCCGAA GGTTGGCGCT GAAGGCTGAA 
TGCAACTCGA AATTAGCTGT TGCCCTATCA ATTATGGAGG AAAGCTTTCA GTCTATGATA 
GATCCAAGAA CTGGTATTGA CATGATACCT CATGTGCTAT ACAACTGGGG GTCAAATTTT 
GCTCGTTTAG ACTTTGATGG GTTTTACACT GTGGTTGTGG AGAAGGACGA TGTGATGATC 
TCAGTAGCAT CTATCAGGGT GCATGGAGTA ACTATTGCAG AGATGCCTCT TGCAGCTACC 
TGCAGCAAAT ATCGCCGACA GGGGATGTGC AGAATCCTCG TGGCTGCCAT CGAAGAGCTG 
CTTATGTCTC TCAAAGTGGA GAAGCTTGTG GTAGCTGCAT TGCCGAGTTT AGTGGAAACT 
TGGACTGAAG GTTTTGGCTT CAAACCCATG GATGATGAAG AACGTGATGC TCTCAAAAGA 
ATAAACTTGA TGGTCTTCCC TGGGACAATT CTGCTCAAGA AAACGCTATA CGAGAGCACA 
AAAACCAACA TTGTGAACGA CGTTTGTCTT TCTAAAATTC AAAGTGAACC ATCAAACAAA 
GAAGCAGACC AAGAGCCAGG CCTAGACAAA GCCGGGTTTC CCATACATAC CAAAGTTGAG 
AGCTGTGATC AGATGGTTCC AGCAGGTTCA GATGAAGACC CGGCACCAGG CTTCCCCGTG 
CCACTTGGAG CCAAGGAAAC AGAACTAACT TCCGAAACAG AGAACCCAAG CCGAGACAGC 
AATGCCGATG ATAGGCCAAA AAAAACAAGA GTATCCATGG GAGAAAAAGA AGAAGAAGAA 
GGTTTGCAGG AAGAGGTATC AAAGATTTCA GAAGAAGGAA AAGAAATGAC AAAAGCTTCT 
TCTTCCTCTG CAGCTTTAGA AGAAGTAAGT GATGTAGGAG TAGTAGTAGT AAATAATGTT 
AGTGATGACA TGTTGCTTTG TTTTGATGAA CAGCTTGACT CTGATAGCTC TGAAGAAGAA 
AACTCGGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104745780 ( XM_010467130.2 ) cDNA ORF clone, Camelina sativa(false flax) -> XP_010465432.1 Camelina sativa increased DNA methylation 1-like (LOC104745780), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa189412
Clone ID Related Accession (Same CDS sequence)	XM_010467129.2 , XM_010467130.2
Accession Version	XM_010467130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Camelina sativa(false flax)
Product	increased DNA methylation 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_025699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010467130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Camelina sativa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCGCA AAGCTGAGAT TGAGATGTTA GGAGGTGACG ACTGCTTTGA AGGGTCTTCT 
GAGGATCATC AGATATTCAG GCAAGTTTTC TTTGGAAGTG ATTCTGGAAA TACTACTAAA 
AGATGTCTCG TTACTGGAGT CATTAACTTC GAATCTGATT CCACTAAGAA TGTTAATTCC 
TCTCTGAGTT CCAACAATGA CAACTCTGTG GTAACAAGTG GGTATGAGGC ATCTGCTTCA 
AAAGATGGTT CAGACTTTAC CACGAAAGCT AAGAGACTGA AGCTTTCAGG TAATAAGCAT 
TTGGATGCAA GGGACGTAAA GGGATCCGCT TTCAGTGGTT TCCCAAATTC GGATATAGCT 
AGAGAAACAA TTCCTCTCCA TTTAGTTGAA TCGTCTAACA AGGGTGTTTC AACTAGCTCT 
TATTTGCTAA AGCAAAGCAT AGAAAAGGGG AAAGAAGTTT ATCTGGGTGG CATTCTCACA 
GAAAAGTGCA AGTCACAAAA CTTGGACAGA TCCGATGGAA AGGAATTTAA AGCAATTGCC 
TCTCCTGTTT CTCAGGAGAG CTTCGCAACC AGAATGATCT GTGCAGGTGC TTCTACGCCT 
CATAGTGAGA AGGCTTGCTT CTCACCGCAG TTGAACAACG GGTCTAAAGT ATCTCCTAAT 
GAGCTAATTA TGTCTAAAAC CTGTCTAAAG ATTGATCCTA AAGATGATCC CCGTCCTCTT 
ATCTACAAAT ATGTCTGTAA GTTGCTAAAT GCTGCCAGAT GGAAAATCGA GAAGCGCGGA 
AGATCAGGTA GGAAATACTG TGATACATTT TACATATCGC CTGAGGGACG GAAATTTCGT 
GAATTTCCGT CAGCTTGGAA ATCGTTGGGT GGGATCCTAT TAGCTGGTCA TAAGTTAATG 
GATACCAGTA CTAAGAAATG GACCGGTATT AATGACTTCT GGTCTGATCT CTCTCTTACA 
CTGTTGGACA TGGAGGAGAA CATGAAAAAT CTAAATCTTG CCAACACACG GGCACTTTGG 
TGGAGTGCCT TGGAGCCTTT TGTGATTGCT GTCTTCATTT CTAAGCAAGT TGGATCTTTA 
AGAAGAGGAA ATAAAGTCGA AGTTGCAAGG ATTTCAATAG TTGACAAGTT GAAGAAAGAA 
GATGCGATTT GTTTGAACAT GATTTCAGGG TGTCCTGAAA GTGTGCTGAC AGTATCTGAA 
GGCAGTCATC TTGTTAATGA CGTGGATGCT ATCCAAGAGA TTCATTCAGA TCTTGAGGTG 
CAAACTAAAA TTGTCCCTCG AAAAGTTTCT TCAAGACTAG AGAGGCAGAA TATAGTTGGT 
AAGGAAATCT ATGGGACTGA TGAGCAGGAG GTTAGCAAGG GCGTTGTAGC AGAAGACATG 
CATGAAAGTG TTATGAGAAA GAAGTTACAC AGAAGATCAA AAAAAGTATC GGATATCAAA 
CCAGTTTCCT TGGACCAACA TGATTCACTA GATTCTAATT CTCTCGACAG CCTTGAATGC 
CAGGATAAGG AGATGAGCAA CATTCACCTC ATCGCAAAGG GAAGTCCGGA TGAAAGGTTG 
AGCAATGAGA AGATTAAGAA GAATCCATGT TGCAATTTAA AGAAAGGAAG AAAGAAAGCT 
AGGAAGCACT ATTCTCAGGA TGATGATCTT ATGGCGTCGA CAATAATCAG AAACAAAGTT 
AAGGTTTGCA GATCAAGCCA GAAAAAGAAA ACCCAGAAAC CAAAGGCTAG AACCAAAAAA 
AGAAACAATA GGGGAGGGTG CAGGCTGCTT CCCCGAAGCA CAAGCAATGT GGAAAATCAA 
TTATGTCAAG GAAATTGGTC TAATTTGGGT CCAAGAACTG TCTTATCATG GTTGATAGCT 
ACCAAAGTAA TTTCCAGGGA CGAAGTAATC CAGTTACGAG ATCCAGATGA TGATACTGTG 
GTAAAAACAG GCCTCGTAAC TAAAGATGGA GTGGTTTGTA CATGTTGCAA CAGGACTGTA 
TCACTTTCTG AATTCAAGAA CCATGCTGGA TTTAACCAGA ATTGTCCATG TCTGAATCTT 
TTCATGGGGT CAGGAAAGCC TTTAGCTTCA TGTCAGCTGG AAGCTTGGTC TGCTGAGTAT 
AAGGCGCGGA GAAATGGATG GAAATCAGTG GAAGCTTCTG ATGATGATCC AAATGATGAT 
TCTTGTGGAG TTTGTGGTGA TGGGGGTGAA CTGATCTGCT GTGATAATTG TCCGTCGACC 
TTCCATCAGG CTTGCTTATC AATGCAGGTG CTCCCTGAAG GAAGTTGGTA TTGCTCGAGC 
TGCACATGTT GGATATGCAG CGAGTTGGTT AGTGATAATG CTGAACGCTA TCAGGATTTT 
AAATGTTCTC ATTGTGCGCA TAAATATCAT GGAATATGTT TGCAAGGGGA AAGTAAACCG 
AGAAAACTTT CTCCAGAGAC CTACTTTTGT GGTAAAAATT GCGAGAAGGT GTACACTGGT 
CTCAGTTCTC GTGTTGGGGT CATTAACCCT AATGCTGCTG GTCTGTCTTG GTCAATTCTG 
AAATGTTTTC AAGAAGATGG AAATGTTCAT TCTGCCCGAA GGTTGGCGCT GAAGGCTGAA 
TGCAACTCGA AATTAGCTGT TGCCCTATCA ATTATGGAGG AAAGCTTTCA GTCTATGATA 
GATCCAAGAA CTGGTATTGA CATGATACCT CATGTGCTAT ACAACTGGGG GTCAAATTTT 
GCTCGTTTAG ACTTTGATGG GTTTTACACT GTGGTTGTGG AGAAGGACGA TGTGATGATC 
TCAGTAGCAT CTATCAGGGT GCATGGAGTA ACTATTGCAG AGATGCCTCT TGCAGCTACC 
TGCAGCAAAT ATCGCCGACA GGGGATGTGC AGAATCCTCG TGGCTGCCAT CGAAGAGCTG 
CTTATGTCTC TCAAAGTGGA GAAGCTTGTG GTAGCTGCAT TGCCGAGTTT AGTGGAAACT 
TGGACTGAAG GTTTTGGCTT CAAACCCATG GATGATGAAG AACGTGATGC TCTCAAAAGA 
ATAAACTTGA TGGTCTTCCC TGGGACAATT CTGCTCAAGA AAACGCTATA CGAGAGCACA 
AAAACCAACA TTGTGAACGA CGTTTGTCTT TCTAAAATTC AAAGTGAACC ATCAAACAAA 
GAAGCAGACC AAGAGCCAGG CCTAGACAAA GCCGGGTTTC CCATACATAC CAAAGTTGAG 
AGCTGTGATC AGATGGTTCC AGCAGGTTCA GATGAAGACC CGGCACCAGG CTTCCCCGTG 
CCACTTGGAG CCAAGGAAAC AGAACTAACT TCCGAAACAG AGAACCCAAG CCGAGACAGC 
AATGCCGATG ATAGGCCAAA AAAAACAAGA GTATCCATGG GAGAAAAAGA AGAAGAAGAA 
GGTTTGCAGG AAGAGGTATC AAAGATTTCA GAAGAAGGAA AAGAAATGAC AAAAGCTTCT 
TCTTCCTCTG CAGCTTTAGA AGAAGTAAGT GATGTAGGAG TAGTAGTAGT AAATAATGTT 
AGTGATGACA TGTTGCTTTG TTTTGATGAA CAGCTTGACT CTGATAGCTC TGAAGAAGAA 
AACTCGGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010465432.1
CDS	516..4067
Translation	MFRKAEIEMLGGDDCFEGSSEDHQIFRQVFFGSDSGNTTKRCLVTGVINFESDSTKNVNSSLSSNNDNSVVTSGYEASASKDGSDFTTKAKRLKLSGNKHLDARDVKGSAFSGFPNSDIARETIPLHLVESSNKGVSTSSYLLKQSIEKGKEVYLGGILTEKCKSQNLDRSDGKEFKAIASPVSQESFATRMICAGASTPHSEKACFSPQLNNGSKVSPNELIMSKTCLKIDPKDDPRPLIYKYVCKLLNAARWKIEKRGRSGRKYCDTFYISPEGRKFREFPSAWKSLGGILLAGHKLMDTSTKKWTGINDFWSDLSLTLLDMEENMKNLNLANTRALWWSALEPFVIAVFISKQVGSLRRGNKVEVARISIVDKLKKEDAICLNMISGCPESVLTVSEGSHLVNDVDAIQEIHSDLEVQTKIVPRKVSSRLERQNIVGKEIYGTDEQEVSKGVVAEDMHESVMRKKLHRRSKKVSDIKPVSLDQHDSLDSNSLDSLECQDKEMSNIHLIAKGSPDERLSNEKIKKNPCCNLKKGRKKARKHYSQDDDLMASTIIRNKVKVCRSSQKKKTQKPKARTKKRNNRGGCRLLPRSTSNVENQLCQGNWSNLGPRTVLSWLIATKVISRDEVIQLRDPDDDTVVKTGLVTKDGVVCTCCNRTVSLSEFKNHAGFNQNCPCLNLFMGSGKPLASCQLEAWSAEYKARRNGWKSVEASDDDPNDDSCGVCGDGGELICCDNCPSTFHQACLSMQVLPEGSWYCSSCTCWICSELVSDNAERYQDFKCSHCAHKYHGICLQGESKPRKLSPETYFCGKNCEKVYTGLSSRVGVINPNAAGLSWSILKCFQEDGNVHSARRLALKAECNSKLAVALSIMEESFQSMIDPRTGIDMIPHVLYNWGSNFARLDFDGFYTVVVEKDDVMISVASIRVHGVTIAEMPLAATCSKYRRQGMCRILVAAIEELLMSLKVEKLVVAALPSLVETWTEGFGFKPMDDEERDALKRINLMVFPGTILLKKTLYESTKTNIVNDVCLSKIQSEPSNKEADQEPGLDKAGFPIHTKVESCDQMVPAGSDEDPAPGFPVPLGAKETELTSETENPSRDSNADDRPKKTRVSMGEKEEEEGLQEEVSKISEEGKEMTKASSSSAALEEVSDVGVVVVNNVSDDMLLCFDEQLDSDSSEEENSE

Target ORF information:

RefSeq Version	XM_010467130.2
Organism	Camelina sativa(false flax)
Definition	Camelina sativa increased DNA methylation 1-like (LOC104745780), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010467130.2

ORF Insert Sequence:

ATGTTTCGCA AAGCTGAGAT TGAGATGTTA GGAGGTGACG ACTGCTTTGA AGGGTCTTCT 
GAGGATCATC AGATATTCAG GCAAGTTTTC TTTGGAAGTG ATTCTGGAAA TACTACTAAA 
AGATGTCTCG TTACTGGAGT CATTAACTTC GAATCTGATT CCACTAAGAA TGTTAATTCC 
TCTCTGAGTT CCAACAATGA CAACTCTGTG GTAACAAGTG GGTATGAGGC ATCTGCTTCA 
AAAGATGGTT CAGACTTTAC CACGAAAGCT AAGAGACTGA AGCTTTCAGG TAATAAGCAT 
TTGGATGCAA GGGACGTAAA GGGATCCGCT TTCAGTGGTT TCCCAAATTC GGATATAGCT 
AGAGAAACAA TTCCTCTCCA TTTAGTTGAA TCGTCTAACA AGGGTGTTTC AACTAGCTCT 
TATTTGCTAA AGCAAAGCAT AGAAAAGGGG AAAGAAGTTT ATCTGGGTGG CATTCTCACA 
GAAAAGTGCA AGTCACAAAA CTTGGACAGA TCCGATGGAA AGGAATTTAA AGCAATTGCC 
TCTCCTGTTT CTCAGGAGAG CTTCGCAACC AGAATGATCT GTGCAGGTGC TTCTACGCCT 
CATAGTGAGA AGGCTTGCTT CTCACCGCAG TTGAACAACG GGTCTAAAGT ATCTCCTAAT 
GAGCTAATTA TGTCTAAAAC CTGTCTAAAG ATTGATCCTA AAGATGATCC CCGTCCTCTT 
ATCTACAAAT ATGTCTGTAA GTTGCTAAAT GCTGCCAGAT GGAAAATCGA GAAGCGCGGA 
AGATCAGGTA GGAAATACTG TGATACATTT TACATATCGC CTGAGGGACG GAAATTTCGT 
GAATTTCCGT CAGCTTGGAA ATCGTTGGGT GGGATCCTAT TAGCTGGTCA TAAGTTAATG 
GATACCAGTA CTAAGAAATG GACCGGTATT AATGACTTCT GGTCTGATCT CTCTCTTACA 
CTGTTGGACA TGGAGGAGAA CATGAAAAAT CTAAATCTTG CCAACACACG GGCACTTTGG 
TGGAGTGCCT TGGAGCCTTT TGTGATTGCT GTCTTCATTT CTAAGCAAGT TGGATCTTTA 
AGAAGAGGAA ATAAAGTCGA AGTTGCAAGG ATTTCAATAG TTGACAAGTT GAAGAAAGAA 
GATGCGATTT GTTTGAACAT GATTTCAGGG TGTCCTGAAA GTGTGCTGAC AGTATCTGAA 
GGCAGTCATC TTGTTAATGA CGTGGATGCT ATCCAAGAGA TTCATTCAGA TCTTGAGGTG 
CAAACTAAAA TTGTCCCTCG AAAAGTTTCT TCAAGACTAG AGAGGCAGAA TATAGTTGGT 
AAGGAAATCT ATGGGACTGA TGAGCAGGAG GTTAGCAAGG GCGTTGTAGC AGAAGACATG 
CATGAAAGTG TTATGAGAAA GAAGTTACAC AGAAGATCAA AAAAAGTATC GGATATCAAA 
CCAGTTTCCT TGGACCAACA TGATTCACTA GATTCTAATT CTCTCGACAG CCTTGAATGC 
CAGGATAAGG AGATGAGCAA CATTCACCTC ATCGCAAAGG GAAGTCCGGA TGAAAGGTTG 
AGCAATGAGA AGATTAAGAA GAATCCATGT TGCAATTTAA AGAAAGGAAG AAAGAAAGCT 
AGGAAGCACT ATTCTCAGGA TGATGATCTT ATGGCGTCGA CAATAATCAG AAACAAAGTT 
AAGGTTTGCA GATCAAGCCA GAAAAAGAAA ACCCAGAAAC CAAAGGCTAG AACCAAAAAA 
AGAAACAATA GGGGAGGGTG CAGGCTGCTT CCCCGAAGCA CAAGCAATGT GGAAAATCAA 
TTATGTCAAG GAAATTGGTC TAATTTGGGT CCAAGAACTG TCTTATCATG GTTGATAGCT 
ACCAAAGTAA TTTCCAGGGA CGAAGTAATC CAGTTACGAG ATCCAGATGA TGATACTGTG 
GTAAAAACAG GCCTCGTAAC TAAAGATGGA GTGGTTTGTA CATGTTGCAA CAGGACTGTA 
TCACTTTCTG AATTCAAGAA CCATGCTGGA TTTAACCAGA ATTGTCCATG TCTGAATCTT 
TTCATGGGGT CAGGAAAGCC TTTAGCTTCA TGTCAGCTGG AAGCTTGGTC TGCTGAGTAT 
AAGGCGCGGA GAAATGGATG GAAATCAGTG GAAGCTTCTG ATGATGATCC AAATGATGAT 
TCTTGTGGAG TTTGTGGTGA TGGGGGTGAA CTGATCTGCT GTGATAATTG TCCGTCGACC 
TTCCATCAGG CTTGCTTATC AATGCAGGTG CTCCCTGAAG GAAGTTGGTA TTGCTCGAGC 
TGCACATGTT GGATATGCAG CGAGTTGGTT AGTGATAATG CTGAACGCTA TCAGGATTTT 
AAATGTTCTC ATTGTGCGCA TAAATATCAT GGAATATGTT TGCAAGGGGA AAGTAAACCG 
AGAAAACTTT CTCCAGAGAC CTACTTTTGT GGTAAAAATT GCGAGAAGGT GTACACTGGT 
CTCAGTTCTC GTGTTGGGGT CATTAACCCT AATGCTGCTG GTCTGTCTTG GTCAATTCTG 
AAATGTTTTC AAGAAGATGG AAATGTTCAT TCTGCCCGAA GGTTGGCGCT GAAGGCTGAA 
TGCAACTCGA AATTAGCTGT TGCCCTATCA ATTATGGAGG AAAGCTTTCA GTCTATGATA 
GATCCAAGAA CTGGTATTGA CATGATACCT CATGTGCTAT ACAACTGGGG GTCAAATTTT 
GCTCGTTTAG ACTTTGATGG GTTTTACACT GTGGTTGTGG AGAAGGACGA TGTGATGATC 
TCAGTAGCAT CTATCAGGGT GCATGGAGTA ACTATTGCAG AGATGCCTCT TGCAGCTACC 
TGCAGCAAAT ATCGCCGACA GGGGATGTGC AGAATCCTCG TGGCTGCCAT CGAAGAGCTG 
CTTATGTCTC TCAAAGTGGA GAAGCTTGTG GTAGCTGCAT TGCCGAGTTT AGTGGAAACT 
TGGACTGAAG GTTTTGGCTT CAAACCCATG GATGATGAAG AACGTGATGC TCTCAAAAGA 
ATAAACTTGA TGGTCTTCCC TGGGACAATT CTGCTCAAGA AAACGCTATA CGAGAGCACA 
AAAACCAACA TTGTGAACGA CGTTTGTCTT TCTAAAATTC AAAGTGAACC ATCAAACAAA 
GAAGCAGACC AAGAGCCAGG CCTAGACAAA GCCGGGTTTC CCATACATAC CAAAGTTGAG 
AGCTGTGATC AGATGGTTCC AGCAGGTTCA GATGAAGACC CGGCACCAGG CTTCCCCGTG 
CCACTTGGAG CCAAGGAAAC AGAACTAACT TCCGAAACAG AGAACCCAAG CCGAGACAGC 
AATGCCGATG ATAGGCCAAA AAAAACAAGA GTATCCATGG GAGAAAAAGA AGAAGAAGAA 
GGTTTGCAGG AAGAGGTATC AAAGATTTCA GAAGAAGGAA AAGAAATGAC AAAAGCTTCT 
TCTTCCTCTG CAGCTTTAGA AGAAGTAAGT GATGTAGGAG TAGTAGTAGT AAATAATGTT 
AGTGATGACA TGTTGCTTTG TTTTGATGAA CAGCTTGACT CTGATAGCTC TGAAGAAGAA 
AACTCGGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104745780 ( XM_010467131.2 ) cDNA ORF clone, Camelina sativa(false flax) -> XP_010465433.1 Camelina sativa increased DNA methylation 1-like (LOC104745780), transcript variant X3, mRNA.

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CloneID	OCa189413
Clone ID Related Accession (Same CDS sequence)	XM_010467131.2
Accession Version	XM_010467131.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Camelina sativa(false flax)
Product	increased DNA methylation 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_025699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010467131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Camelina sativa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCGCA AAGCTGAGAT TGAGATGTTA GGAGGTGACG ACTGCTTTGA AGGGTCTTCT 
GAGGATCATC AGATATTCAG GCAAGTTTTC TTTGGAAGTG ATTCTGGAAA TACTACTAAA 
AGATGTCTCG TTACTGGAGT CATTAACTTC GAATCTGATT CCACTAAGAA TGTTAATTCC 
TCTCTGAGTT CCAACAATGA CAACTCTGTG GTAACAAGTG GGTATGAGGC ATCTGCTTCA 
AAAGATGGTT CAGACTTTAC CACGAAAGCT AAGAGACTGA AGCTTTCAGG TAATAAGCAT 
TTGGATGCAA GGGACGTAAA GGGATCCGCT TTCAGTGGTT TCCCAAATTC GGATATAGCT 
AGAGAAACAA TTCCTCTCCA TTTAGTTGAA TCGTCTAACA AGGGTGTTTC AACTAGCTCT 
TATTTGCTAA AGCAAAGCAT AGAAAAGGGG AAAGAAGTTT ATCTGGGTGG CATTCTCACA 
GAAAAGTGCA AGTCACAAAA CTTGGACAGA TCCGATGGAA AGGAATTTAA AGCAATTGCC 
TCTCCTGTTT CTCAGGAGAG CTTCGCAACC AGAATGATCT GTGCAGGTGC TTCTACGCCT 
CATAGTGAGA AGGCTTGCTT CTCACCGCAG TTGAACAACG GGTCTAAAGT ATCTCCTAAT 
GAGCTAATTA TGTCTAAAAC CTGTCTAAAG ATTGATCCTA AAGATGATCC CCGTCCTCTT 
ATCTACAAAT ATGTCTGTAA GTTGCTAAAT GCTGCCAGAT GGAAAATCGA GAAGCGCGGA 
AGATCAGGTA GGAAATACTG TGATACATTT TACATATCGC CTGAGGGACG GAAATTTCGT 
GAATTTCCGT CAGCTTGGAA ATCGTTGGGT GGGATCCTAT TAGCTGGTCA TAAGTTAATG 
GATACCAGTA CTAAGAAATG GACCGGTATT AATGACTTCT GGTCTGATCT CTCTCTTACA 
CTGTTGGACA TGGAGGAGAA CATGAAAAAT CTAAATCTTG CCAACACACG GGCACTTTGG 
TGGAGTGCCT TGGAGCCTTT TGTGATTGCT GTCTTCATTT CTAAGCAAGT TGGATCTTTA 
AGAAGAGGAA ATAAAGTCGA AGTTGCAAGG ATTTCAATAG TTGACAAGTT GAAGAAAGAA 
GATGCGATTT GTTTGAACAT GATTTCAGGG TGTCCTGAAA GTGTGCTGAC AGTATCTGAA 
GGCAGTCATC TTGTTAATGA CGTGGATGCT ATCCAAGAGA TTCATTCAGA TCTTGAGGTG 
CAAACTAAAA TTGTCCCTCG AAAAGTTTCT TCAAGACTAG AGAGGCAGAA TATAGTTGGT 
AAGGAAATCT ATGGGACTGA TGAGCAGGAG GTTAGCAAGG GCGTTGTTAG CAAGGGCGTT 
GTAGCAGAAG ACATGCATGA AAGTGTTATG AGAAAGAAGT TACACAGAAG ATCAAAAAAA 
GTATCGGATA TCAAACCAGT TTCCTTGGAC CAACATGATT CACTAGATTC TAATTCTCTC 
GACAGCCTTG AATGCCAGGA TAAGGAGATG AGCAACATTC ACCTCATCGC AAAGGGAAGT 
CCGGATGAAA GGTTGAGCAA TGAGAAGATT AAGAAGAATC CATGTTGCAA TTTAAAGAAA 
GGAAGAAAGA AAGCTAGGAA GCACTATTCT CAGGATGATG ATCTTATGGC GTCGACAATA 
ATCAGAAACA AAGTTAAGGT TTGCAGATCA AGCCAGAAAA AGAAAACCCA GAAACCAAAG 
GCTAGAACCA AAAAAAGAAA CAATAGGGGA GGGTGCAGGC TGCTTCCCCG AAGCACAAGC 
AATGTGGAAA ATCAATTATG TCAAGGAAAT TGGTCTAATT TGGGTCCAAG AACTGTCTTA 
TCATGGTTGA TAGCTACCAA AGTAATTTCC AGGGACGAAG TAATCCAGTT ACGAGATCCA 
GATGATGATA CTGTGGTAAA AACAGGCCTC GTAACTAAAG ATGGAGTGGT TTGTACATGT 
TGCAACAGGA CTGTATCACT TTCTGAATTC AAGAACCATG CTGGATTTAA CCAGAATTGT 
CCATGTCTGA ATCTTTTCAT GGGGTCAGGA AAGCCTTTAG CTTCATGTCA GCTGGAAGCT 
TGGTCTGCTG AGTATAAGGC GCGGAGAAAT GGATGGAAAT CAGTGGAAGC TTCTGATGAT 
GATCCAAATG ATGATTCTTG TGGAGTTTGT GGTGATGGGG GTGAACTGAT CTGCTGTGAT 
AATTGTCCGT CGACCTTCCA TCAGGCTTGC TTATCAATGC AGGTGCTCCC TGAAGGAAGT 
TGGTATTGCT CGAGCTGCAC ATGTTGGATA TGCAGCGAGT TGGTTAGTGA TAATGCTGAA 
CGCTATCAGG ATTTTAAATG TTCTCATTGT GCGCATAAAT ATCATGGAAT ATGTTTGCAA 
GGGGAAAGTA AACCGAGAAA ACTTTCTCCA GAGACCTACT TTTGTGGTAA AAATTGCGAG 
AAGGTGTACA CTGGTCTCAG TTCTCGTGTT GGGGTCATTA ACCCTAATGC TGCTGGTCTG 
TCTTGGTCAA TTCTGAAATG TTTTCAAGAA GATGGAAATG TTCATTCTGC CCGAAGGTTG 
GCGCTGAAGG CTGAATGCAA CTCGAAATTA GCTGTTGCCC TATCAATTAT GGAGGAAAGC 
TTTCAGTCTA TGATAGATCC AAGAACTGGT ATTGACATGA TACCTCATGT GCTATACAAC 
TGGGGGTCAA ATTTTGCTCG TTTAGACTTT GATGGGTTTT ACACTGTGGT TGTGGAGAAG 
GACGATGTGA TGATCTCAGT AGCATCTATC AGGGTGCATG GAGTAACTAT TGCAGAGATG 
CCTCTTGCAG CTACCTGCAG CAAATATCGC CGACAGGGGA TGTGCAGAAT CCTCGTGGCT 
GCCATCGAAG AGCTGCTTAT GTCTCTCAAA GTGGAGAAGC TTGTGGTAGC TGCATTGCCG 
AGTTTAGTGG AAACTTGGAC TGAAGGTTTT GGCTTCAAAC CCATGGATGA TGAAGAACGT 
GATGCTCTCA AAAGAATAAA CTTGATGGTC TTCCCTGGGA CAATTCTGCT CAAGAAAACG 
CTATACGAGA GCACAAAAAC CAACATTGTG AACGACGTTT GTCTTTCTAA AATTCAAAGT 
GAACCATCAA ACAAAGAAGC AGACCAAGAG CCAGGCCTAG ACAAAGCCGG GTTTCCCATA 
CATACCAAAG TTGAGAGCTG TGATCAGATG GTTCCAGCAG GTTCAGATGA AGACCCGGCA 
CCAGGCTTCC CCGTGCCACT TGGAGCCAAG GAAACAGAAC TAACTTCCGA AACAGAGAAC 
CCAAGCCGAG ACAGCAATGC CGATGATAGG CCAAAAAAAA CAAGAGTATC CATGGGAGAA 
AAAGAAGAAG AAGAAGGTTT GCAGGAAGAG GTATCAAAGA TTTCAGAAGA AGGAAAAGAA 
ATGACAAAAG CTTCTTCTTC CTCTGCAGCT TTAGAAGAAG TAAGTGATGT AGGAGTAGTA 
GTAGTAAATA ATGTTAGTGA TGACATGTTG CTTTGTTTTG ATGAACAGCT TGACTCTGAT 
AGCTCTGAAG AAGAAAACTC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010465433.1
CDS	541..4107
Translation	MFRKAEIEMLGGDDCFEGSSEDHQIFRQVFFGSDSGNTTKRCLVTGVINFESDSTKNVNSSLSSNNDNSVVTSGYEASASKDGSDFTTKAKRLKLSGNKHLDARDVKGSAFSGFPNSDIARETIPLHLVESSNKGVSTSSYLLKQSIEKGKEVYLGGILTEKCKSQNLDRSDGKEFKAIASPVSQESFATRMICAGASTPHSEKACFSPQLNNGSKVSPNELIMSKTCLKIDPKDDPRPLIYKYVCKLLNAARWKIEKRGRSGRKYCDTFYISPEGRKFREFPSAWKSLGGILLAGHKLMDTSTKKWTGINDFWSDLSLTLLDMEENMKNLNLANTRALWWSALEPFVIAVFISKQVGSLRRGNKVEVARISIVDKLKKEDAICLNMISGCPESVLTVSEGSHLVNDVDAIQEIHSDLEVQTKIVPRKVSSRLERQNIVGKEIYGTDEQEVSKGVVSKGVVAEDMHESVMRKKLHRRSKKVSDIKPVSLDQHDSLDSNSLDSLECQDKEMSNIHLIAKGSPDERLSNEKIKKNPCCNLKKGRKKARKHYSQDDDLMASTIIRNKVKVCRSSQKKKTQKPKARTKKRNNRGGCRLLPRSTSNVENQLCQGNWSNLGPRTVLSWLIATKVISRDEVIQLRDPDDDTVVKTGLVTKDGVVCTCCNRTVSLSEFKNHAGFNQNCPCLNLFMGSGKPLASCQLEAWSAEYKARRNGWKSVEASDDDPNDDSCGVCGDGGELICCDNCPSTFHQACLSMQVLPEGSWYCSSCTCWICSELVSDNAERYQDFKCSHCAHKYHGICLQGESKPRKLSPETYFCGKNCEKVYTGLSSRVGVINPNAAGLSWSILKCFQEDGNVHSARRLALKAECNSKLAVALSIMEESFQSMIDPRTGIDMIPHVLYNWGSNFARLDFDGFYTVVVEKDDVMISVASIRVHGVTIAEMPLAATCSKYRRQGMCRILVAAIEELLMSLKVEKLVVAALPSLVETWTEGFGFKPMDDEERDALKRINLMVFPGTILLKKTLYESTKTNIVNDVCLSKIQSEPSNKEADQEPGLDKAGFPIHTKVESCDQMVPAGSDEDPAPGFPVPLGAKETELTSETENPSRDSNADDRPKKTRVSMGEKEEEEGLQEEVSKISEEGKEMTKASSSSAALEEVSDVGVVVVNNVSDDMLLCFDEQLDSDSSEEENSE

Target ORF information:

RefSeq Version	XM_010467131.2
Organism	Camelina sativa(false flax)
Definition	Camelina sativa increased DNA methylation 1-like (LOC104745780), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010467131.2

ORF Insert Sequence:

ATGTTTCGCA AAGCTGAGAT TGAGATGTTA GGAGGTGACG ACTGCTTTGA AGGGTCTTCT 
GAGGATCATC AGATATTCAG GCAAGTTTTC TTTGGAAGTG ATTCTGGAAA TACTACTAAA 
AGATGTCTCG TTACTGGAGT CATTAACTTC GAATCTGATT CCACTAAGAA TGTTAATTCC 
TCTCTGAGTT CCAACAATGA CAACTCTGTG GTAACAAGTG GGTATGAGGC ATCTGCTTCA 
AAAGATGGTT CAGACTTTAC CACGAAAGCT AAGAGACTGA AGCTTTCAGG TAATAAGCAT 
TTGGATGCAA GGGACGTAAA GGGATCCGCT TTCAGTGGTT TCCCAAATTC GGATATAGCT 
AGAGAAACAA TTCCTCTCCA TTTAGTTGAA TCGTCTAACA AGGGTGTTTC AACTAGCTCT 
TATTTGCTAA AGCAAAGCAT AGAAAAGGGG AAAGAAGTTT ATCTGGGTGG CATTCTCACA 
GAAAAGTGCA AGTCACAAAA CTTGGACAGA TCCGATGGAA AGGAATTTAA AGCAATTGCC 
TCTCCTGTTT CTCAGGAGAG CTTCGCAACC AGAATGATCT GTGCAGGTGC TTCTACGCCT 
CATAGTGAGA AGGCTTGCTT CTCACCGCAG TTGAACAACG GGTCTAAAGT ATCTCCTAAT 
GAGCTAATTA TGTCTAAAAC CTGTCTAAAG ATTGATCCTA AAGATGATCC CCGTCCTCTT 
ATCTACAAAT ATGTCTGTAA GTTGCTAAAT GCTGCCAGAT GGAAAATCGA GAAGCGCGGA 
AGATCAGGTA GGAAATACTG TGATACATTT TACATATCGC CTGAGGGACG GAAATTTCGT 
GAATTTCCGT CAGCTTGGAA ATCGTTGGGT GGGATCCTAT TAGCTGGTCA TAAGTTAATG 
GATACCAGTA CTAAGAAATG GACCGGTATT AATGACTTCT GGTCTGATCT CTCTCTTACA 
CTGTTGGACA TGGAGGAGAA CATGAAAAAT CTAAATCTTG CCAACACACG GGCACTTTGG 
TGGAGTGCCT TGGAGCCTTT TGTGATTGCT GTCTTCATTT CTAAGCAAGT TGGATCTTTA 
AGAAGAGGAA ATAAAGTCGA AGTTGCAAGG ATTTCAATAG TTGACAAGTT GAAGAAAGAA 
GATGCGATTT GTTTGAACAT GATTTCAGGG TGTCCTGAAA GTGTGCTGAC AGTATCTGAA 
GGCAGTCATC TTGTTAATGA CGTGGATGCT ATCCAAGAGA TTCATTCAGA TCTTGAGGTG 
CAAACTAAAA TTGTCCCTCG AAAAGTTTCT TCAAGACTAG AGAGGCAGAA TATAGTTGGT 
AAGGAAATCT ATGGGACTGA TGAGCAGGAG GTTAGCAAGG GCGTTGTTAG CAAGGGCGTT 
GTAGCAGAAG ACATGCATGA AAGTGTTATG AGAAAGAAGT TACACAGAAG ATCAAAAAAA 
GTATCGGATA TCAAACCAGT TTCCTTGGAC CAACATGATT CACTAGATTC TAATTCTCTC 
GACAGCCTTG AATGCCAGGA TAAGGAGATG AGCAACATTC ACCTCATCGC AAAGGGAAGT 
CCGGATGAAA GGTTGAGCAA TGAGAAGATT AAGAAGAATC CATGTTGCAA TTTAAAGAAA 
GGAAGAAAGA AAGCTAGGAA GCACTATTCT CAGGATGATG ATCTTATGGC GTCGACAATA 
ATCAGAAACA AAGTTAAGGT TTGCAGATCA AGCCAGAAAA AGAAAACCCA GAAACCAAAG 
GCTAGAACCA AAAAAAGAAA CAATAGGGGA GGGTGCAGGC TGCTTCCCCG AAGCACAAGC 
AATGTGGAAA ATCAATTATG TCAAGGAAAT TGGTCTAATT TGGGTCCAAG AACTGTCTTA 
TCATGGTTGA TAGCTACCAA AGTAATTTCC AGGGACGAAG TAATCCAGTT ACGAGATCCA 
GATGATGATA CTGTGGTAAA AACAGGCCTC GTAACTAAAG ATGGAGTGGT TTGTACATGT 
TGCAACAGGA CTGTATCACT TTCTGAATTC AAGAACCATG CTGGATTTAA CCAGAATTGT 
CCATGTCTGA ATCTTTTCAT GGGGTCAGGA AAGCCTTTAG CTTCATGTCA GCTGGAAGCT 
TGGTCTGCTG AGTATAAGGC GCGGAGAAAT GGATGGAAAT CAGTGGAAGC TTCTGATGAT 
GATCCAAATG ATGATTCTTG TGGAGTTTGT GGTGATGGGG GTGAACTGAT CTGCTGTGAT 
AATTGTCCGT CGACCTTCCA TCAGGCTTGC TTATCAATGC AGGTGCTCCC TGAAGGAAGT 
TGGTATTGCT CGAGCTGCAC ATGTTGGATA TGCAGCGAGT TGGTTAGTGA TAATGCTGAA 
CGCTATCAGG ATTTTAAATG TTCTCATTGT GCGCATAAAT ATCATGGAAT ATGTTTGCAA 
GGGGAAAGTA AACCGAGAAA ACTTTCTCCA GAGACCTACT TTTGTGGTAA AAATTGCGAG 
AAGGTGTACA CTGGTCTCAG TTCTCGTGTT GGGGTCATTA ACCCTAATGC TGCTGGTCTG 
TCTTGGTCAA TTCTGAAATG TTTTCAAGAA GATGGAAATG TTCATTCTGC CCGAAGGTTG 
GCGCTGAAGG CTGAATGCAA CTCGAAATTA GCTGTTGCCC TATCAATTAT GGAGGAAAGC 
TTTCAGTCTA TGATAGATCC AAGAACTGGT ATTGACATGA TACCTCATGT GCTATACAAC 
TGGGGGTCAA ATTTTGCTCG TTTAGACTTT GATGGGTTTT ACACTGTGGT TGTGGAGAAG 
GACGATGTGA TGATCTCAGT AGCATCTATC AGGGTGCATG GAGTAACTAT TGCAGAGATG 
CCTCTTGCAG CTACCTGCAG CAAATATCGC CGACAGGGGA TGTGCAGAAT CCTCGTGGCT 
GCCATCGAAG AGCTGCTTAT GTCTCTCAAA GTGGAGAAGC TTGTGGTAGC TGCATTGCCG 
AGTTTAGTGG AAACTTGGAC TGAAGGTTTT GGCTTCAAAC CCATGGATGA TGAAGAACGT 
GATGCTCTCA AAAGAATAAA CTTGATGGTC TTCCCTGGGA CAATTCTGCT CAAGAAAACG 
CTATACGAGA GCACAAAAAC CAACATTGTG AACGACGTTT GTCTTTCTAA AATTCAAAGT 
GAACCATCAA ACAAAGAAGC AGACCAAGAG CCAGGCCTAG ACAAAGCCGG GTTTCCCATA 
CATACCAAAG TTGAGAGCTG TGATCAGATG GTTCCAGCAG GTTCAGATGA AGACCCGGCA 
CCAGGCTTCC CCGTGCCACT TGGAGCCAAG GAAACAGAAC TAACTTCCGA AACAGAGAAC 
CCAAGCCGAG ACAGCAATGC CGATGATAGG CCAAAAAAAA CAAGAGTATC CATGGGAGAA 
AAAGAAGAAG AAGAAGGTTT GCAGGAAGAG GTATCAAAGA TTTCAGAAGA AGGAAAAGAA 
ATGACAAAAG CTTCTTCTTC CTCTGCAGCT TTAGAAGAAG TAAGTGATGT AGGAGTAGTA 
GTAGTAAATA ATGTTAGTGA TGACATGTTG CTTTGTTTTG ATGAACAGCT TGACTCTGAT 
AGCTCTGAAG AAGAAAACTC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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LOC104745780 cDNA ORF clone, Camelina sativa(false flax)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム