SFI1 cDNA ORF clone, Calypte anna(Anna's hummingbird)

Gene
Clones
gRNAs

Summary

Gene Symbol	SFI1
Entrez Gene ID	103536678
Full Name	SFI1 centrin binding protein
Gene Type	protein-coding
Organism	Calypte anna(Anna's hummingbird)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008502873.1	XP_008501095.1	protein SFI1 homolog
XM_030460700.1	XP_030316560.1	protein SFI1 homolog

Pathways from BioSystems
Gene Ontology

The following SFI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SFI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa428150	XM_008502873.1 Latest version!	Calypte anna Sfi1 homolog, spindle assembly associated (yeast) (SFI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa811341	XM_030460700.1 Latest version!	Calypte anna SFI1 centrin binding protein (SFI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SFI1 ( XM_008502873.1 ) cDNA ORF clone, Calypte anna(Anna's hummingbird) -> XP_008501095.1 Calypte anna Sfi1 homolog, spindle assembly associated (yeast) (SFI1), mRNA.

Price & Availability↑

CloneID	OCa428150
Clone ID Related Accession (Same CDS sequence)	XM_008502873.1
Accession Version	XM_008502873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-07-09
Organism	Calypte anna(Anna's hummingbird)
Product	protein SFI1 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007622184.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calypte anna Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCATGAAG TCCAACAACT TCCTAGGCAT AAAATGCATG CAACTGGGCA ATCTGTTACT 
CCCCAGTCTG CCAGAGAAGT TGGGTATACG TGGAATCAAA GAAGAAGATT GAAAGAATTC 
CAAGCCAGAT ATCTAGCAAG GAAATTCTTC TATGTGTGGG CAAAAAAGAC ATTTGGACAA 
ATTCTCCCTT CCACAGCCAG ATGCTTTTAT GACCAGAAGA TTCTTCAGAA AACATTTGGA 
CAGTGGAAGG ACTTGTGGAC TGCTCGTAGA GAAAGGAAGC TCAGCCTCAG AGCAGAGCAC 
CATTACAGGC ACTTACTCCA TAATTTGGTA TTCAAGGCTT GGAGAGCCTA TGTGTGCCAG 
CAGCAAGGGA AGAGGAACAA GTACTGTGTT GCAGAGTCTC ATGCAAAGAA GCAAAACTTG 
CTCAGGACGT GGCAATGCTG GGTGGTTTAT GTCCGTGTTC AAAGGACCAA GAGTGGGATG 
CAGTCTGTGG CACTGGCATT CAGGGAAAGA AGCTGTTTGA GCAGGTGGTG GGCAGTGTGG 
AGACGACAGC ACTACCAGAA CTGCACTGAA CACAGAATCA ATGTTCTGGC TCTGCAGCAT 
TGGGCCCAGA GCCTGCAATT TCGAGCTTGG TTGCAGTGGA GGGAGCTGTA TTTGTACAGC 
CAGAATGACA AGCAGAAAGC AACCAGGGCA GTAACTCACC ATCAGCACTG GGTACTGAAG 
AGATGCATGG AAGCTTGGCT GAGGTACTTA AACCTCCATA AAGCAAAGAA ACATCAAAAT 
GAGGTAGCCC AAGAGCACCA CAGGAGCAGG GTAGTCCAGC AGTGTTTCTC TGACTGGTGG 
CTGTCATGGG ACTGCAGGAG GAGAATGCGT GCTCACCAGG AAGACACTGA GAGACTGGCA 
GGAAGGATTG CCATAAGGAG AGTCTTTGCA CACTGGAAGC ATTATGTGCT GTGTGTGGAA 
GAGATTCGAC AGCAGGAACT GGCTGAAAAC CACTACAAGC ATCATCTGTT GAAACTTGGG 
TTTAAGGCTT TTTGTCAGAA TGTCATCACC ACTCATCTTC AGCAAATGAG AAAAAATCTG 
GCACACCGTC AGCATCAAGT TACAGTGCTG AAGAAGTTCT GGAACTGTTG GAAGTCCCAT 
TTGGAAAAGA AGGAAGAAGA GCAGCAGCAG GCCTTAACAG CAGTGGCTCA TGCCCGTTAT 
AGGAGGGTGT TGATGAGACA GGTATTTAAC ACATGGCTGC TGAACACCTG CAAGCAGCAA 
GAATACAGAA CTGAAGAGAA GAGGGCTGTA CTTCATTTTG AGAGGCAGCT ATTGCGTGGC 
TTCTGGTGCT CCTGGCGCAG GAGAACAGCT GCATGTTTAG AGGAGCAAGA GGGCTTGGTT 
CGTGCAAGAG GTCACTACAG TAACCTGCTG CTGCTAAAGG CTTTCTGTCT GTGGAAACAA 
AATACTCAGG AGAGAAAAAC AGAGACACTT AAGGAGAGGC AAGCCTTACG TTTTCATGAT 
TCAAAGTGTC TGCAGCAGTC TTGGAACAAG TGGAGGGAGT ACCTGGAACA TCAACGTGAG 
AAATGGAGGA AGTTGGTAGA AGCAGACGTG CATTATCAGC ACACATTGCT GGGCAAGACC 
TTTGCAGCCT GGAAGATTTA CCAACATAAC ATCCAGTGCA TTATGTACCA AGTAGATGAA 
AAGGAGAAAC AGCACACTAG GCTACTGCTG CGGCAGCTTC TGTGTACATG GAGAGAAAAT 
GCCCTTGCTC TTATATGTGA AGCTAAGGCA ACTTCTCGAG CAGATGAGCA CTACAGGAGA 
GCAATCCTGT TAAAGGTGTT GCTGCAGTGG AGATCTGCTG CATCACTGCA AGGGTATTAC 
CGTCAGCAGA AGATGACAGC TGTGATGGAG GCCAGAAAAC AGTTGGATCT AGTGCATTTA 
CAGAACCTGT TTCTTCACTG GAAGAGATTA ACCAGGGAGT CTCTGCTGGC AAGGGCTCAG 
CAGTACAGGG CAGTACAGCA CCACCAGCAG CATGTACTCC AGAAGTATCT GGTGAAGTGG 
AAGAAATATC ATCAGCTGTG CCTTGAGAAA ATGCTACTGC AGAGACAGGG AGATCAGCTA 
ATGGCTTATA GGCTTTGCTC TGCTTCCTTC TCTTGTTGGA AGACACAGCT GTTGCAGCAG 
CGATGGGAAA AGCAGAAGAC AGTGCAAGCC CTGTGGCACT GGTCCCTTTC ATTGCAGAGA 
AAGGTATTTG ATGCTTGGCT CCTGTTTGCC AAGGGAAAAC AGCAGAAGGA AGGTGATATT 
GAAAAAGCCA CAGGACTTTA CCATACTACT TCTCTGAGAG AAAGCTATGC TGCTGGCATC 
AAGCAGTTGC AGGGACAGCT CCAAGCCCAG CACCAGCTGA AGAAGCAGGT GACATTTAAA 
ATGCCCAATG CTACCCCTGA GACAAGAGAA CATTCAACAG AGAAAGAGCC ACGGCCTTCA 
AAATGCAATC ATCTGCCATT TGACCTTGAT GATGGGAACT CAATTCTTCA AGATCTATCA 
GAAGCACCCT TTCAGCAAAT ATCTGTGAAA AAAAGTTCTG CACAGACTGG GAATTTAATG 
CTAGCACCTC TGCTGGAAAA AAGTAAACGT CTGTCTCAAA GTGGCGATAC CTCTCCTTCC 
TGTCCATCTC TCCCATCTGT TTCACTGGGG ACACATGATG TGCACCATGC TGTTCAGAGG 
CCAGAGCTAT GGTCTTCTTC ACCTTTTATA ACCCAGATGA AAACTTGGGG AGAAGTGAGA 
GGCAGTCAGC CTGGGAGTGG TCACAATGGA GCCCAGTGCC AGGACTCTCA GCAGGAGACT 
GTGGAGAAGT TGCAACCAAA GTTACAGTCA CGTTTGCTGT CACCTGAAGA AGTGGTGGGA 
GAAAGGAGTC ACCAAACTGC AGGGCAAGAA GGGGAGCACA GTTTCAGAGA AGAAATGGTG 
TTACAGAGAC AGCTAGAAGG TGAACTTCGA CACATCCAAC AGCAGATGCA GTACTACTAC 
AGCAAGAAAC AAGAACTCAG GTCCTGTCAG CAGCAGGCAC AGATTCTACA GAAGTGGCTA 
GAAAATAGCA CACAATCAGA GGACAGAGGT GGTGTACAAG AGCTTAAGGA AGAATTGCAT 
CAGATGCAGG TGAGGATCAG CACTCTCAGC AAAGCACAAC CAAGAGACAG ACAGCATGTG 
CAGAAGCTGC TTGCCAGACT GCAAGATATC GAGATCACGC TGGAGACATA CTGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008501095.1
CDS	1..3240
Translation	MHEVQQLPRHKMHATGQSVTPQSAREVGYTWNQRRRLKEFQARYLARKFFYVWAKKTFGQILPSTARCFYDQKILQKTFGQWKDLWTARRERKLSLRAEHHYRHLLHNLVFKAWRAYVCQQQGKRNKYCVAESHAKKQNLLRTWQCWVVYVRVQRTKSGMQSVALAFRERSCLSRWWAVWRRQHYQNCTEHRINVLALQHWAQSLQFRAWLQWRELYLYSQNDKQKATRAVTHHQHWVLKRCMEAWLRYLNLHKAKKHQNEVAQEHHRSRVVQQCFSDWWLSWDCRRRMRAHQEDTERLAGRIAIRRVFAHWKHYVLCVEEIRQQELAENHYKHHLLKLGFKAFCQNVITTHLQQMRKNLAHRQHQVTVLKKFWNCWKSHLEKKEEEQQQALTAVAHARYRRVLMRQVFNTWLLNTCKQQEYRTEEKRAVLHFERQLLRGFWCSWRRRTAACLEEQEGLVRARGHYSNLLLLKAFCLWKQNTQERKTETLKERQALRFHDSKCLQQSWNKWREYLEHQREKWRKLVEADVHYQHTLLGKTFAAWKIYQHNIQCIMYQVDEKEKQHTRLLLRQLLCTWRENALALICEAKATSRADEHYRRAILLKVLLQWRSAASLQGYYRQQKMTAVMEARKQLDLVHLQNLFLHWKRLTRESLLARAQQYRAVQHHQQHVLQKYLVKWKKYHQLCLEKMLLQRQGDQLMAYRLCSASFSCWKTQLLQQRWEKQKTVQALWHWSLSLQRKVFDAWLLFAKGKQQKEGDIEKATGLYHTTSLRESYAAGIKQLQGQLQAQHQLKKQVTFKMPNATPETREHSTEKEPRPSKCNHLPFDLDDGNSILQDLSEAPFQQISVKKSSAQTGNLMLAPLLEKSKRLSQSGDTSPSCPSLPSVSLGTHDVHHAVQRPELWSSSPFITQMKTWGEVRGSQPGSGHNGAQCQDSQQETVEKLQPKLQSRLLSPEEVVGERSHQTAGQEGEHSFREEMVLQRQLEGELRHIQQQMQYYYSKKQELRSCQQQAQILQKWLENSTQSEDRGGVQELKEELHQMQVRISTLSKAQPRDRQHVQKLLARLQDIEITLETYCT

Target ORF information:

RefSeq Version	XM_008502873.1
Organism	Calypte anna(Anna's hummingbird)
Definition	Calypte anna Sfi1 homolog, spindle assembly associated (yeast) (SFI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008502873.1

ORF Insert Sequence:

ATGCATGAAG TCCAACAACT TCCTAGGCAT AAAATGCATG CAACTGGGCA ATCTGTTACT 
CCCCAGTCTG CCAGAGAAGT TGGGTATACG TGGAATCAAA GAAGAAGATT GAAAGAATTC 
CAAGCCAGAT ATCTAGCAAG GAAATTCTTC TATGTGTGGG CAAAAAAGAC ATTTGGACAA 
ATTCTCCCTT CCACAGCCAG ATGCTTTTAT GACCAGAAGA TTCTTCAGAA AACATTTGGA 
CAGTGGAAGG ACTTGTGGAC TGCTCGTAGA GAAAGGAAGC TCAGCCTCAG AGCAGAGCAC 
CATTACAGGC ACTTACTCCA TAATTTGGTA TTCAAGGCTT GGAGAGCCTA TGTGTGCCAG 
CAGCAAGGGA AGAGGAACAA GTACTGTGTT GCAGAGTCTC ATGCAAAGAA GCAAAACTTG 
CTCAGGACGT GGCAATGCTG GGTGGTTTAT GTCCGTGTTC AAAGGACCAA GAGTGGGATG 
CAGTCTGTGG CACTGGCATT CAGGGAAAGA AGCTGTTTGA GCAGGTGGTG GGCAGTGTGG 
AGACGACAGC ACTACCAGAA CTGCACTGAA CACAGAATCA ATGTTCTGGC TCTGCAGCAT 
TGGGCCCAGA GCCTGCAATT TCGAGCTTGG TTGCAGTGGA GGGAGCTGTA TTTGTACAGC 
CAGAATGACA AGCAGAAAGC AACCAGGGCA GTAACTCACC ATCAGCACTG GGTACTGAAG 
AGATGCATGG AAGCTTGGCT GAGGTACTTA AACCTCCATA AAGCAAAGAA ACATCAAAAT 
GAGGTAGCCC AAGAGCACCA CAGGAGCAGG GTAGTCCAGC AGTGTTTCTC TGACTGGTGG 
CTGTCATGGG ACTGCAGGAG GAGAATGCGT GCTCACCAGG AAGACACTGA GAGACTGGCA 
GGAAGGATTG CCATAAGGAG AGTCTTTGCA CACTGGAAGC ATTATGTGCT GTGTGTGGAA 
GAGATTCGAC AGCAGGAACT GGCTGAAAAC CACTACAAGC ATCATCTGTT GAAACTTGGG 
TTTAAGGCTT TTTGTCAGAA TGTCATCACC ACTCATCTTC AGCAAATGAG AAAAAATCTG 
GCACACCGTC AGCATCAAGT TACAGTGCTG AAGAAGTTCT GGAACTGTTG GAAGTCCCAT 
TTGGAAAAGA AGGAAGAAGA GCAGCAGCAG GCCTTAACAG CAGTGGCTCA TGCCCGTTAT 
AGGAGGGTGT TGATGAGACA GGTATTTAAC ACATGGCTGC TGAACACCTG CAAGCAGCAA 
GAATACAGAA CTGAAGAGAA GAGGGCTGTA CTTCATTTTG AGAGGCAGCT ATTGCGTGGC 
TTCTGGTGCT CCTGGCGCAG GAGAACAGCT GCATGTTTAG AGGAGCAAGA GGGCTTGGTT 
CGTGCAAGAG GTCACTACAG TAACCTGCTG CTGCTAAAGG CTTTCTGTCT GTGGAAACAA 
AATACTCAGG AGAGAAAAAC AGAGACACTT AAGGAGAGGC AAGCCTTACG TTTTCATGAT 
TCAAAGTGTC TGCAGCAGTC TTGGAACAAG TGGAGGGAGT ACCTGGAACA TCAACGTGAG 
AAATGGAGGA AGTTGGTAGA AGCAGACGTG CATTATCAGC ACACATTGCT GGGCAAGACC 
TTTGCAGCCT GGAAGATTTA CCAACATAAC ATCCAGTGCA TTATGTACCA AGTAGATGAA 
AAGGAGAAAC AGCACACTAG GCTACTGCTG CGGCAGCTTC TGTGTACATG GAGAGAAAAT 
GCCCTTGCTC TTATATGTGA AGCTAAGGCA ACTTCTCGAG CAGATGAGCA CTACAGGAGA 
GCAATCCTGT TAAAGGTGTT GCTGCAGTGG AGATCTGCTG CATCACTGCA AGGGTATTAC 
CGTCAGCAGA AGATGACAGC TGTGATGGAG GCCAGAAAAC AGTTGGATCT AGTGCATTTA 
CAGAACCTGT TTCTTCACTG GAAGAGATTA ACCAGGGAGT CTCTGCTGGC AAGGGCTCAG 
CAGTACAGGG CAGTACAGCA CCACCAGCAG CATGTACTCC AGAAGTATCT GGTGAAGTGG 
AAGAAATATC ATCAGCTGTG CCTTGAGAAA ATGCTACTGC AGAGACAGGG AGATCAGCTA 
ATGGCTTATA GGCTTTGCTC TGCTTCCTTC TCTTGTTGGA AGACACAGCT GTTGCAGCAG 
CGATGGGAAA AGCAGAAGAC AGTGCAAGCC CTGTGGCACT GGTCCCTTTC ATTGCAGAGA 
AAGGTATTTG ATGCTTGGCT CCTGTTTGCC AAGGGAAAAC AGCAGAAGGA AGGTGATATT 
GAAAAAGCCA CAGGACTTTA CCATACTACT TCTCTGAGAG AAAGCTATGC TGCTGGCATC 
AAGCAGTTGC AGGGACAGCT CCAAGCCCAG CACCAGCTGA AGAAGCAGGT GACATTTAAA 
ATGCCCAATG CTACCCCTGA GACAAGAGAA CATTCAACAG AGAAAGAGCC ACGGCCTTCA 
AAATGCAATC ATCTGCCATT TGACCTTGAT GATGGGAACT CAATTCTTCA AGATCTATCA 
GAAGCACCCT TTCAGCAAAT ATCTGTGAAA AAAAGTTCTG CACAGACTGG GAATTTAATG 
CTAGCACCTC TGCTGGAAAA AAGTAAACGT CTGTCTCAAA GTGGCGATAC CTCTCCTTCC 
TGTCCATCTC TCCCATCTGT TTCACTGGGG ACACATGATG TGCACCATGC TGTTCAGAGG 
CCAGAGCTAT GGTCTTCTTC ACCTTTTATA ACCCAGATGA AAACTTGGGG AGAAGTGAGA 
GGCAGTCAGC CTGGGAGTGG TCACAATGGA GCCCAGTGCC AGGACTCTCA GCAGGAGACT 
GTGGAGAAGT TGCAACCAAA GTTACAGTCA CGTTTGCTGT CACCTGAAGA AGTGGTGGGA 
GAAAGGAGTC ACCAAACTGC AGGGCAAGAA GGGGAGCACA GTTTCAGAGA AGAAATGGTG 
TTACAGAGAC AGCTAGAAGG TGAACTTCGA CACATCCAAC AGCAGATGCA GTACTACTAC 
AGCAAGAAAC AAGAACTCAG GTCCTGTCAG CAGCAGGCAC AGATTCTACA GAAGTGGCTA 
GAAAATAGCA CACAATCAGA GGACAGAGGT GGTGTACAAG AGCTTAAGGA AGAATTGCAT 
CAGATGCAGG TGAGGATCAG CACTCTCAGC AAAGCACAAC CAAGAGACAG ACAGCATGTG 
CAGAAGCTGC TTGCCAGACT GCAAGATATC GAGATCACGC TGGAGACATA CTGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SFI1 ( XM_030460700.1 ) cDNA ORF clone, Calypte anna(Anna's hummingbird) -> XP_030316560.1 Calypte anna SFI1 centrin binding protein (SFI1), mRNA.

Price & Availability↑

CloneID	OCa811341
Clone ID Related Accession (Same CDS sequence)	XM_030460700.1
Accession Version	XM_030460700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-19
Organism	Calypte anna(Anna's hummingbird)
Product	protein SFI1 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044261.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Calypte anna Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 16% of CDS bases ##RefSeq-Attributes-END##

ATGGTTACCG CAAAGCCTGG GACCGGGGAC CTAAATAACA ACAAAACCGA GCCCTCAGAA 
CGCCAGACAC CGCTTCAAGC CACGCAGCCC CGCCTGGGGC ACGAACCGGC ACCCCGAGCC 
CCGCCAGACC CGCAGCCCAG CTCCGCGGCA GCCTGCCCAC AACAAAGCTG CCCCGTGGGG 
GCCATCGGGA GCAGAGCACC GTGGCCGGGG GAGCGGGGCC GCCGGGACAC CCTCACCGCG 
GCTCCCCTCG CGTGGCGGCG GGGGCAGCGC GGCCCAGGCC CGGTCCCGAG GCAGCCGAGC 
CGAGGGGGCA GGAGGGCGGC GGGAAAGGTG ACTCAGCGGA TCCTCCCCCC CCCCCCCAGC 
CCCGCCGGCC GTACTCACCG CCCCGCCGGC GGCTCTCCCA ACGGCGCCCC GCTGAAGGCC 
TTACCGCCGC CCGCGGCGTT TCCTGATCCC GGCCACCGGC TCCGCTCGGC CCCTGCCCGT 
CCCTCCACTG GCGGCTCCCG GCGCCGCTCC CGCCGCTTTC CGCGCCACCC GCGCTCCGGG 
CCGGTCCCGT CCCCGCCCCG CCCGCCGCAG CTCGCGCGGC CCCGGCCCCC TCCGCGCTGG 
CTCGCGCGCC CGCTCCTTAT TCGCCCGACT CTGTCTCTCT TCAGCCGCCG TAACCGGGGC 
CTTCCGCTGC CCGCCCCGCA CCCATTTAAT GGCCCGCGCG CAGGCTGGGG GAGCGCCGAG 
GGCCCGCAGG CACTCTCGTC AGACAGCCCT GCGGGGGAGG CTGTCGCCGG GAGCAGCGGC 
GGCGGGACGT GTTGGAAATC CCTGGAGTTG CACGAAGTCC AACAACTTCC TAGGCATAAA 
ATGCATGCAA CTGGGCAATC TGTTACTCCC CAGTCTGCCA GAGAAGTTGG GTATACGTGG 
AATCAAAGAA GAAGATTGAA AGAATTCCAA GCCAGATATC TAGCAAGGAA ATTCTTCTAT 
TTGTGGGCAA AAAAGACATT TGGACAAATT CTCCCTTCCA CAGCCAGATG CTTTTATGAC 
CAGAAGATTC TTCAGAAAAC ATTTGGACAG TGGAAGGACT TGTGGACTGC TTGTAGAGAA 
AGGAAGCTCA GCCTCAGAGC AGAGCACCAT TACAGGCACT TACTCCATAA TTTGGTATTC 
AAGGCTTGGA GAGCCTATGT GTGCCAGCAG CAAGGGAAGA GGAACAAGTA CTGTGTTGCA 
GAGTCTCATG CAAAGAAGCA AAACTTGCTC AGGACGTGGC AATGCTGGGT GGTTTATGTC 
CGTGTTCAAA GGACCAAGAG TGGGATGCAG TCTGTGGCAC TGGCATTCAG GGAAAGAAGC 
TGTTTGAGCA GGTGGTGGGC AGTGTGGAGA CGACAGCACT ACCAGAACTG CACTGAACAC 
AGAATCAATG TTCTGGCTCT GCAGCATTGG GCCCAGAGCC TGCAATTTCG AGCTTGGTTG 
CAGTGGAGGG AGCTGTATTT GTACAGCCAG AATGACAAGC AGAAAGCAAC CAGGGCAGTA 
ACTCACCATC AGCACTGGGT ACTGAAGAGA TGCATGGAAG CTTGGCTGAG GTACTTAAAC 
CTCCATAAAG CAAAGAAACA TCAAAATGAG GTAGCCCAAG AGCACCACAG GAGCAGGGTA 
GTCCAGCAGT GTTTCTCTGA CTGGTGGCTG TCATGGGACT GCAGGAGGAG AATGCGTGCT 
CACCAGGAAG ACACTGAGAG ACTGGCAGGA AGGATTGCCA TAAGGAGAGT CTTTGCACAC 
TGGAAGCATT ATGTGCTGTG TGTGGAAGAG ATTCGACAGC AGGAACTGGC TGAAAACCAC 
TACAAGCATC ATCTGTTGAA ACTTGGGTTT AAGGCTTTTT GTCAGAATGT CATCACCACT 
CATCTTCAGC AAATGAGAAA AAATCTGGCA CACCGTCAGC ATCAAGTTAC AGTGCTGAAG 
AAGTTCTGGA ACTGTTGGAA GTCCCATTTG GAAAAGAAGG AAGAAGAGCA GCAGCAGGCC 
TTAACAGCAG TGGCTCATGC CCGTTATAGG AGGGTGTTGA TGAGACAGGT ATTTAACACA 
TGGCTGCTGA ACACCTGCAA GCAGCAAGAA TACAGAACTG AAGAGAAGAG GGCTGTACTT 
CATTTTGAGA GGCAGCTATT GCGTGGCTTC TGGTGCTCCT GGCGCAGGAG AACAGCTGCA 
TGTTTAGAGG AGCAAGAGGG CTTGGTTCGT GCAAGAGGTC ACTACAGTAA CCTGCTGCTG 
CTAAAGGCTT TCTGTCTGTG GAAACAAAAT ACTCAGGAGA GAAAAACAGA GACACTTAAG 
GAGAGGCAAG CCTTACGTTT TCATGATTCA AAGTGTCTGC AGCAGTCTTG GAACAAGTGG 
AGGGAGTACC TGGAACATCA ACGTGAGAAA TGGAGGAAGT TGGTAGAAGC AGACGTGCAT 
TATCAGCACA CATTGCTGGG CAAGACCTTT GCAGCCTGGA AGATTTACCA ACATAACATC 
CAGTGCATTA TGTACCAAGT AGATGAAAAG GAGAAACAGC ACACTAGGCT ACTGCTGCGG 
CAGCTTCTGT GTACATGGAG AGAAAATGCC CTTGCTCTTA TATGTGAAGC TAAGGCAACT 
TCTCGAGCAG ATGAGCACTA CAGGAGAGCA ATCCTGTTAA AGGTGTTGCT GCAGTGGAGA 
TCTGCTGCAT CACTGCAAGG GTATTACCGT CAGCAGAAGA TGACAGCTGT GATGGAGGCC 
AGAAAACAGT TGGATCTAGT GCATTTACAG AACCTGTTTC TTCACTGGAA GAGATTAACC 
AGGGAGTCTC TGCTGGCAAG GGCTCAGCAG TACAGGGCAG TACAGCACCA CCAGCAGCAT 
GTACTCCAGA AGTATCTGGT GAAGTGGAAG AAATATCATC AGCTGTGCCT TGAGAAAATG 
CTACTGCAGA GACAGGGAGA TCAGCTAATG GCTTATAGGC TTTGCTCTGC TTCCTTCTCT 
TGTTGGAAGA CACAGCTGTT GCAGCAGCGA TGGGAAAAGC AGAAGACAGT GCAAGCCCTG 
TGGCACTGGT CCCTTTCATT GCAGAGAAAG GTATTTGATG CTTGGCTCCT GTTTGCCAAG 
GGAAAACAGC AGAAGGAAGG TGATATTGAA AAAGCCACAG GACTTTACCA TACTACTTCT 
CTGAGAGAAA GCTATGCTGC TGGCATCAAG CAGTTGCAGG GACAGCTCCA AGCCCAGCAC 
CAGCTGAAGG CAGCCCACAA CCTTCACCTG TCAGTGTATC ACTGTGCCCT GCTATGGAAA 
CAGAAGGCAC TCTCCAAGAA ATTAAATAAC CCTTGTTCTT TTCTGCCACC TCTCAAGAAG 
CAGGTGACAT TTAAAATGCC CAATGCTACC CCTGAGACAA GAGAACATTC AACAGAGAAA 
GAGCCACGGC CTTCAAAATG CAATCATCTG CCATTTGACC TTGATGATGG GAACTCAATT 
CTTCAAGATC TAAATGCTAG TCAACAAGCA AGGTTACGAC CACAGAGGTC TGACTTCCTT 
CTGGAATCTC TAGAAAGCAA GGAACTTCTG GGACCTCCTT TTACTAGATC AGAAGCACCC 
TTTCAGCAAA TATCTGTGAA AAAAAGTTCT GCACAGACTG GGAATTTAAT GCTAGCACCT 
CTGCTGGAAA AAAGTAAACG TCTGTCTCAA AGTGGCGATA CCTCTCCTTC CTGTCCATCT 
CTCCCATCTG TTTCACTGGG GACACATGAT GTGCACCATG CTGTTCAGAG GCCAGAGCTA 
TGGTCTTCTT CACCTTTTAT AACCCAGATG AAAACTTGGG GAGAAGTGAG AGGCAGTCAG 
CCTGGGAGTG GTCACAATGG AGCCCAGTGC CAGGACTCTC AGCAGGAGAC TGTGGAGAAG 
TTGCAACCAA AGTTACAGTC ACGTTTGCTG TCACCTGAAG AAGTGGTGGG AGAAAGGAGT 
CACCAAACTG CAGGGCAAGA AGGGGAGCAC AGTTTCAGAG AAGAAATGGT GTTACAGAGA 
CAGCTAGAAG GTGAACTTCG ACACATCCAA CAGCAGATGC AGTACTACTA CAGCAAGAAA 
CAAGAACTCA GGTCCTGTCA GCAGCAGGCA CAGATTCTAC AGAAGTGGCT AGAAAATAGC 
ACACAATCAG AGGACAGAGG TGGTGTACAA GAGCTTAAGG AAGAATTGCA TCAGATGCAG 
GTGAGGATCA GCACTCTCAG CAAAGCACAA CCAAGAGACA GACAGCATGT GCAGAAGCTG 
CTTGCCAGAC TGCAAGATAT CGAGATCACG CTGGAGACAT ACTGCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030316560.1
CDS	1..4251
Translation	MVTAKPGTGDLNNNKTEPSERQTPLQATQPRLGHEPAPRAPPDPQPSSAAACPQQSCPVGAIGSRAPWPGERGRRDTLTAAPLAWRRGQRGPGPVPRQPSRGGRRAAGKVTQRILPPPPSPAGRTHRPAGGSPNGAPLKALPPPAAFPDPGHRLRSAPARPSTGGSRRRSRRFPRHPRSGPVPSPPRPPQLARPRPPPRWLARPLLIRPTLSLFSRRNRGLPLPAPHPFNGPRAGWGSAEGPQALSSDSPAGEAVAGSSGGGTCWKSLELHEVQQLPRHKMHATGQSVTPQSAREVGYTWNQRRRLKEFQARYLARKFFYLWAKKTFGQILPSTARCFYDQKILQKTFGQWKDLWTACRERKLSLRAEHHYRHLLHNLVFKAWRAYVCQQQGKRNKYCVAESHAKKQNLLRTWQCWVVYVRVQRTKSGMQSVALAFRERSCLSRWWAVWRRQHYQNCTEHRINVLALQHWAQSLQFRAWLQWRELYLYSQNDKQKATRAVTHHQHWVLKRCMEAWLRYLNLHKAKKHQNEVAQEHHRSRVVQQCFSDWWLSWDCRRRMRAHQEDTERLAGRIAIRRVFAHWKHYVLCVEEIRQQELAENHYKHHLLKLGFKAFCQNVITTHLQQMRKNLAHRQHQVTVLKKFWNCWKSHLEKKEEEQQQALTAVAHARYRRVLMRQVFNTWLLNTCKQQEYRTEEKRAVLHFERQLLRGFWCSWRRRTAACLEEQEGLVRARGHYSNLLLLKAFCLWKQNTQERKTETLKERQALRFHDSKCLQQSWNKWREYLEHQREKWRKLVEADVHYQHTLLGKTFAAWKIYQHNIQCIMYQVDEKEKQHTRLLLRQLLCTWRENALALICEAKATSRADEHYRRAILLKVLLQWRSAASLQGYYRQQKMTAVMEARKQLDLVHLQNLFLHWKRLTRESLLARAQQYRAVQHHQQHVLQKYLVKWKKYHQLCLEKMLLQRQGDQLMAYRLCSASFSCWKTQLLQQRWEKQKTVQALWHWSLSLQRKVFDAWLLFAKGKQQKEGDIEKATGLYHTTSLRESYAAGIKQLQGQLQAQHQLKAAHNLHLSVYHCALLWKQKALSKKLNNPCSFLPPLKKQVTFKMPNATPETREHSTEKEPRPSKCNHLPFDLDDGNSILQDLNASQQARLRPQRSDFLLESLESKELLGPPFTRSEAPFQQISVKKSSAQTGNLMLAPLLEKSKRLSQSGDTSPSCPSLPSVSLGTHDVHHAVQRPELWSSSPFITQMKTWGEVRGSQPGSGHNGAQCQDSQQETVEKLQPKLQSRLLSPEEVVGERSHQTAGQEGEHSFREEMVLQRQLEGELRHIQQQMQYYYSKKQELRSCQQQAQILQKWLENSTQSEDRGGVQELKEELHQMQVRISTLSKAQPRDRQHVQKLLARLQDIEITLETYCT

Target ORF information:

RefSeq Version	XM_030460700.1
Organism	Calypte anna(Anna's hummingbird)
Definition	Calypte anna SFI1 centrin binding protein (SFI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030460700.1

ORF Insert Sequence:

ATGGTTACCG CAAAGCCTGG GACCGGGGAC CTAAATAACA ACAAAACCGA GCCCTCAGAA 
CGCCAGACAC CGCTTCAAGC CACGCAGCCC CGCCTGGGGC ACGAACCGGC ACCCCGAGCC 
CCGCCAGACC CGCAGCCCAG CTCCGCGGCA GCCTGCCCAC AACAAAGCTG CCCCGTGGGG 
GCCATCGGGA GCAGAGCACC GTGGCCGGGG GAGCGGGGCC GCCGGGACAC CCTCACCGCG 
GCTCCCCTCG CGTGGCGGCG GGGGCAGCGC GGCCCAGGCC CGGTCCCGAG GCAGCCGAGC 
CGAGGGGGCA GGAGGGCGGC GGGAAAGGTG ACTCAGCGGA TCCTCCCCCC CCCCCCCAGC 
CCCGCCGGCC GTACTCACCG CCCCGCCGGC GGCTCTCCCA ACGGCGCCCC GCTGAAGGCC 
TTACCGCCGC CCGCGGCGTT TCCTGATCCC GGCCACCGGC TCCGCTCGGC CCCTGCCCGT 
CCCTCCACTG GCGGCTCCCG GCGCCGCTCC CGCCGCTTTC CGCGCCACCC GCGCTCCGGG 
CCGGTCCCGT CCCCGCCCCG CCCGCCGCAG CTCGCGCGGC CCCGGCCCCC TCCGCGCTGG 
CTCGCGCGCC CGCTCCTTAT TCGCCCGACT CTGTCTCTCT TCAGCCGCCG TAACCGGGGC 
CTTCCGCTGC CCGCCCCGCA CCCATTTAAT GGCCCGCGCG CAGGCTGGGG GAGCGCCGAG 
GGCCCGCAGG CACTCTCGTC AGACAGCCCT GCGGGGGAGG CTGTCGCCGG GAGCAGCGGC 
GGCGGGACGT GTTGGAAATC CCTGGAGTTG CACGAAGTCC AACAACTTCC TAGGCATAAA 
ATGCATGCAA CTGGGCAATC TGTTACTCCC CAGTCTGCCA GAGAAGTTGG GTATACGTGG 
AATCAAAGAA GAAGATTGAA AGAATTCCAA GCCAGATATC TAGCAAGGAA ATTCTTCTAT 
TTGTGGGCAA AAAAGACATT TGGACAAATT CTCCCTTCCA CAGCCAGATG CTTTTATGAC 
CAGAAGATTC TTCAGAAAAC ATTTGGACAG TGGAAGGACT TGTGGACTGC TTGTAGAGAA 
AGGAAGCTCA GCCTCAGAGC AGAGCACCAT TACAGGCACT TACTCCATAA TTTGGTATTC 
AAGGCTTGGA GAGCCTATGT GTGCCAGCAG CAAGGGAAGA GGAACAAGTA CTGTGTTGCA 
GAGTCTCATG CAAAGAAGCA AAACTTGCTC AGGACGTGGC AATGCTGGGT GGTTTATGTC 
CGTGTTCAAA GGACCAAGAG TGGGATGCAG TCTGTGGCAC TGGCATTCAG GGAAAGAAGC 
TGTTTGAGCA GGTGGTGGGC AGTGTGGAGA CGACAGCACT ACCAGAACTG CACTGAACAC 
AGAATCAATG TTCTGGCTCT GCAGCATTGG GCCCAGAGCC TGCAATTTCG AGCTTGGTTG 
CAGTGGAGGG AGCTGTATTT GTACAGCCAG AATGACAAGC AGAAAGCAAC CAGGGCAGTA 
ACTCACCATC AGCACTGGGT ACTGAAGAGA TGCATGGAAG CTTGGCTGAG GTACTTAAAC 
CTCCATAAAG CAAAGAAACA TCAAAATGAG GTAGCCCAAG AGCACCACAG GAGCAGGGTA 
GTCCAGCAGT GTTTCTCTGA CTGGTGGCTG TCATGGGACT GCAGGAGGAG AATGCGTGCT 
CACCAGGAAG ACACTGAGAG ACTGGCAGGA AGGATTGCCA TAAGGAGAGT CTTTGCACAC 
TGGAAGCATT ATGTGCTGTG TGTGGAAGAG ATTCGACAGC AGGAACTGGC TGAAAACCAC 
TACAAGCATC ATCTGTTGAA ACTTGGGTTT AAGGCTTTTT GTCAGAATGT CATCACCACT 
CATCTTCAGC AAATGAGAAA AAATCTGGCA CACCGTCAGC ATCAAGTTAC AGTGCTGAAG 
AAGTTCTGGA ACTGTTGGAA GTCCCATTTG GAAAAGAAGG AAGAAGAGCA GCAGCAGGCC 
TTAACAGCAG TGGCTCATGC CCGTTATAGG AGGGTGTTGA TGAGACAGGT ATTTAACACA 
TGGCTGCTGA ACACCTGCAA GCAGCAAGAA TACAGAACTG AAGAGAAGAG GGCTGTACTT 
CATTTTGAGA GGCAGCTATT GCGTGGCTTC TGGTGCTCCT GGCGCAGGAG AACAGCTGCA 
TGTTTAGAGG AGCAAGAGGG CTTGGTTCGT GCAAGAGGTC ACTACAGTAA CCTGCTGCTG 
CTAAAGGCTT TCTGTCTGTG GAAACAAAAT ACTCAGGAGA GAAAAACAGA GACACTTAAG 
GAGAGGCAAG CCTTACGTTT TCATGATTCA AAGTGTCTGC AGCAGTCTTG GAACAAGTGG 
AGGGAGTACC TGGAACATCA ACGTGAGAAA TGGAGGAAGT TGGTAGAAGC AGACGTGCAT 
TATCAGCACA CATTGCTGGG CAAGACCTTT GCAGCCTGGA AGATTTACCA ACATAACATC 
CAGTGCATTA TGTACCAAGT AGATGAAAAG GAGAAACAGC ACACTAGGCT ACTGCTGCGG 
CAGCTTCTGT GTACATGGAG AGAAAATGCC CTTGCTCTTA TATGTGAAGC TAAGGCAACT 
TCTCGAGCAG ATGAGCACTA CAGGAGAGCA ATCCTGTTAA AGGTGTTGCT GCAGTGGAGA 
TCTGCTGCAT CACTGCAAGG GTATTACCGT CAGCAGAAGA TGACAGCTGT GATGGAGGCC 
AGAAAACAGT TGGATCTAGT GCATTTACAG AACCTGTTTC TTCACTGGAA GAGATTAACC 
AGGGAGTCTC TGCTGGCAAG GGCTCAGCAG TACAGGGCAG TACAGCACCA CCAGCAGCAT 
GTACTCCAGA AGTATCTGGT GAAGTGGAAG AAATATCATC AGCTGTGCCT TGAGAAAATG 
CTACTGCAGA GACAGGGAGA TCAGCTAATG GCTTATAGGC TTTGCTCTGC TTCCTTCTCT 
TGTTGGAAGA CACAGCTGTT GCAGCAGCGA TGGGAAAAGC AGAAGACAGT GCAAGCCCTG 
TGGCACTGGT CCCTTTCATT GCAGAGAAAG GTATTTGATG CTTGGCTCCT GTTTGCCAAG 
GGAAAACAGC AGAAGGAAGG TGATATTGAA AAAGCCACAG GACTTTACCA TACTACTTCT 
CTGAGAGAAA GCTATGCTGC TGGCATCAAG CAGTTGCAGG GACAGCTCCA AGCCCAGCAC 
CAGCTGAAGG CAGCCCACAA CCTTCACCTG TCAGTGTATC ACTGTGCCCT GCTATGGAAA 
CAGAAGGCAC TCTCCAAGAA ATTAAATAAC CCTTGTTCTT TTCTGCCACC TCTCAAGAAG 
CAGGTGACAT TTAAAATGCC CAATGCTACC CCTGAGACAA GAGAACATTC AACAGAGAAA 
GAGCCACGGC CTTCAAAATG CAATCATCTG CCATTTGACC TTGATGATGG GAACTCAATT 
CTTCAAGATC TAAATGCTAG TCAACAAGCA AGGTTACGAC CACAGAGGTC TGACTTCCTT 
CTGGAATCTC TAGAAAGCAA GGAACTTCTG GGACCTCCTT TTACTAGATC AGAAGCACCC 
TTTCAGCAAA TATCTGTGAA AAAAAGTTCT GCACAGACTG GGAATTTAAT GCTAGCACCT 
CTGCTGGAAA AAAGTAAACG TCTGTCTCAA AGTGGCGATA CCTCTCCTTC CTGTCCATCT 
CTCCCATCTG TTTCACTGGG GACACATGAT GTGCACCATG CTGTTCAGAG GCCAGAGCTA 
TGGTCTTCTT CACCTTTTAT AACCCAGATG AAAACTTGGG GAGAAGTGAG AGGCAGTCAG 
CCTGGGAGTG GTCACAATGG AGCCCAGTGC CAGGACTCTC AGCAGGAGAC TGTGGAGAAG 
TTGCAACCAA AGTTACAGTC ACGTTTGCTG TCACCTGAAG AAGTGGTGGG AGAAAGGAGT 
CACCAAACTG CAGGGCAAGA AGGGGAGCAC AGTTTCAGAG AAGAAATGGT GTTACAGAGA 
CAGCTAGAAG GTGAACTTCG ACACATCCAA CAGCAGATGC AGTACTACTA CAGCAAGAAA 
CAAGAACTCA GGTCCTGTCA GCAGCAGGCA CAGATTCTAC AGAAGTGGCT AGAAAATAGC 
ACACAATCAG AGGACAGAGG TGGTGTACAA GAGCTTAAGG AAGAATTGCA TCAGATGCAG 
GTGAGGATCA GCACTCTCAG CAAAGCACAA CCAAGAGACA GACAGCATGT GCAGAAGCTG 
CTTGCCAGAC TGCAAGATAT CGAGATCACG CTGGAGACAT ACTGCACCTG A