rtcb cDNA ORF clone, Callorhinchus milii(elephant shark)

Gene
Clones
gRNAs

Summary

Gene Symbol	rtcb
Entrez Gene ID	103181817
Full Name	RNA 2',3'-cyclic phosphate and 5'-OH ligase
Gene Type	protein-coding
Organism	Callorhinchus milii(elephant shark)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001293028.1	NP_001279957.1	tRNA-splicing ligase RtcB homolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequencing and analysis of full-length cDNAs, 5'-ESTs and 3'-ESTs from a cartilaginous fish, the elephant shark (Callorhinchus milii).
Tan YY, Kodzius R, Tay BH, Tay A, Brenner S, Venkatesh B
PloS one7(10)e47174(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rtcb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rtcb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa121564	NM_001293028.1 Latest version!	Callorhinchus milii RNA 2',3'-cyclic phosphate and 5'-OH ligase (rtcb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rtcb ( NM_001293028.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> NP_001279957.1 Callorhinchus milii RNA 2',3'-cyclic phosphate and 5'-OH ligase (rtcb), mRNA.

Price & Availability↑

CloneID	OCa121564
Clone ID Related Accession (Same CDS sequence)	NM_001293028.1
Accession Version	NM_001293028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Callorhinchus milii(elephant shark)
Product	tRNA-splicing ligase RtcB homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JX053439.1. On May 23, 2014 this sequence version replaced XM_007898528.1. ##Evidence-Data-START## Transcript exon combination :: JX053439.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00188955, SAMN02699929 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCGCA GCTACAATGA CGAGCTCGGC TTCTTGGAGA AGGTCCGCAA GAACTGCTGG 
AGGATCAACA AGGGCTTCGT GCCCAACATG CAGGTGGAAG GTGTGTTTTA TGTCAATGAA 
CCCCTGGAGA AATTGATGTT TGAAGAATTA CGAAACTCTT GTCGTGGTGG AGGTGTGGGT 
GGCTTCCTAC CAGCTATGAA GCAGATTGGG AATGTGGCAG CGTTGCCTGG GATTATTCAT 
AAATCCATTG GACTTCCTGA TGTCCACTCG GGTTATGGGT TTGCAATTGG GAACATGGCA 
GCTTTTGACA TGAATGATCC CCAGTCTGTT GTTTCGCCGG GTGGTGTGGG GTTTGATATA 
AACTGCGGAG TGCGCTTGCT GCGAACTAAC CTCGACGAAG GGGACGTGCA GCCAGTGAAG 
GAACAGCTGG CCCAGGCCAT GTTTGATCAC ATTCCCGTAG GGGTGGGCTC GAAGGGAGTC 
ATCCCAATGG GCGCCAAGGA CCTGGAGGAA GCTTTGGAGA TGGGGGTGGA CTGGTCTCTG 
AGGGAAGGTT ATGCATGGGC TGAGGACAAG GAGCACTGTG AAGAATATGG CAGAATGCTG 
CAGGCTGATC CCAGCAAGGT CTCTTCAAAG GCTAAAAAGA GAGGATTGCC TCAGCTGGGG 
ACTCTGGGCG CTGGGAACCA CTATGCTGAG ATTCAGGTTG TCGACGAGAT CTTTGATGAA 
TATGCAGCAC GTAAAATGGG CATTGACCAC AAGGGACAGG TGTGTGTGAT GATTCACTGT 
GGCAGCAGAG GTCTTGGACA TCAGGTCGCT ACAGATGCCC TTGTGGCCAT GGAGAAAGCC 
ATGAAGAGAG ATAAGATCAT TGTCAACGAT CGACAGCTAG CATGTGCCAG GATCAACTCC 
CAGGAAGGCC AGGACTATCT GAAGGGCATG GCAGCAGCTG GAGACTATGC CTGGGTGAAT 
CGCTCCTCCA TGACATTCTT GACACGACAG GCTTTTGCCA AAGTTTTCGG CACTACTCCA 
GATGACTTGG ATATGCACAT GATTTATGAT GTATCTCACA ACATAGCTAA GGTCGAGGAG 
CACGTTGTGG ATGGGAAACA GAAGACTCTG CTGGTTCACA GGAAGGGTTC TACCAGAGCC 
TTCCCACCAC ATCACCCTCT CATTGCAGTC GATTATCAGC TGACTGGTCA GCCTGTTCTG 
ATCGGTGGCA CAATGGGAAC CTGTAGCTAC GTCCTTACAG GCACAGAGCA AGGAATGACG 
GAGACATTTG GAACGACCTG CCATGGAGCG GGCCGAGCCT TGTCCCGTGC CAAATCCCGG 
CGTAACCTTG ATTTCCAAGA TGTGTTGGAT AAACTATCAG ATATGGGCAT TGCCATTCGT 
GTCGCCTCCC CCAAATTGGT GATGGAAGAA GCACCTGAAT CCTACAAGAA TGTAACTGAT 
GTGGTGAATA CTTGTCATGA TGCAGGGATC AGCAAAAAGG CTATTAAACT TCGACCTATT 
GCAGTTATAA AGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001279957.1
CDS	19..1536
Translation	MSRSYNDELGFLEKVRKNCWRINKGFVPNMQVEGVFYVNEPLEKLMFEELRNSCRGGGVGGFLPAMKQIGNVAALPGIIHKSIGLPDVHSGYGFAIGNMAAFDMNDPQSVVSPGGVGFDINCGVRLLRTNLDEGDVQPVKEQLAQAMFDHIPVGVGSKGVIPMGAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPSKVSSKAKKRGLPQLGTLGAGNHYAEIQVVDEIFDEYAARKMGIDHKGQVCVMIHCGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARINSQEGQDYLKGMAAAGDYAWVNRSSMTFLTRQAFAKVFGTTPDDLDMHMIYDVSHNIAKVEEHVVDGKQKTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLSDMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGISKKAIKLRPIAVIKG

Target ORF information:

RefSeq Version	NM_001293028.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii RNA 2',3'-cyclic phosphate and 5'-OH ligase (rtcb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293028.1

ORF Insert Sequence:

ATGAGCCGCA GCTACAATGA CGAGCTCGGC TTCTTGGAGA AGGTCCGCAA GAACTGCTGG 
AGGATCAACA AGGGCTTCGT GCCCAACATG CAGGTGGAAG GTGTGTTTTA TGTCAATGAA 
CCCCTGGAGA AATTGATGTT TGAAGAATTA CGAAACTCTT GTCGTGGTGG AGGTGTGGGT 
GGCTTCCTAC CAGCTATGAA GCAGATTGGG AATGTGGCAG CGTTGCCTGG GATTATTCAT 
AAATCCATTG GACTTCCTGA TGTCCACTCG GGTTATGGGT TTGCAATTGG GAACATGGCA 
GCTTTTGACA TGAATGATCC CCAGTCTGTT GTTTCGCCGG GTGGTGTGGG GTTTGATATA 
AACTGCGGAG TGCGCTTGCT GCGAACTAAC CTCGACGAAG GGGACGTGCA GCCAGTGAAG 
GAACAGCTGG CCCAGGCCAT GTTTGATCAC ATTCCCGTAG GGGTGGGCTC GAAGGGAGTC 
ATCCCAATGG GCGCCAAGGA CCTGGAGGAA GCTTTGGAGA TGGGGGTGGA CTGGTCTCTG 
AGGGAAGGTT ATGCATGGGC TGAGGACAAG GAGCACTGTG AAGAATATGG CAGAATGCTG 
CAGGCTGATC CCAGCAAGGT CTCTTCAAAG GCTAAAAAGA GAGGATTGCC TCAGCTGGGG 
ACTCTGGGCG CTGGGAACCA CTATGCTGAG ATTCAGGTTG TCGACGAGAT CTTTGATGAA 
TATGCAGCAC GTAAAATGGG CATTGACCAC AAGGGACAGG TGTGTGTGAT GATTCACTGT 
GGCAGCAGAG GTCTTGGACA TCAGGTCGCT ACAGATGCCC TTGTGGCCAT GGAGAAAGCC 
ATGAAGAGAG ATAAGATCAT TGTCAACGAT CGACAGCTAG CATGTGCCAG GATCAACTCC 
CAGGAAGGCC AGGACTATCT GAAGGGCATG GCAGCAGCTG GAGACTATGC CTGGGTGAAT 
CGCTCCTCCA TGACATTCTT GACACGACAG GCTTTTGCCA AAGTTTTCGG CACTACTCCA 
GATGACTTGG ATATGCACAT GATTTATGAT GTATCTCACA ACATAGCTAA GGTCGAGGAG 
CACGTTGTGG ATGGGAAACA GAAGACTCTG CTGGTTCACA GGAAGGGTTC TACCAGAGCC 
TTCCCACCAC ATCACCCTCT CATTGCAGTC GATTATCAGC TGACTGGTCA GCCTGTTCTG 
ATCGGTGGCA CAATGGGAAC CTGTAGCTAC GTCCTTACAG GCACAGAGCA AGGAATGACG 
GAGACATTTG GAACGACCTG CCATGGAGCG GGCCGAGCCT TGTCCCGTGC CAAATCCCGG 
CGTAACCTTG ATTTCCAAGA TGTGTTGGAT AAACTATCAG ATATGGGCAT TGCCATTCGT 
GTCGCCTCCC CCAAATTGGT GATGGAAGAA GCACCTGAAT CCTACAAGAA TGTAACTGAT 
GTGGTGAATA CTTGTCATGA TGCAGGGATC AGCAAAAAGG CTATTAAACT TCGACCTATT 
GCAGTTATAA AGGGTTGA