gclc cDNA ORF clone, Callorhinchus milii(elephant shark)

Gene
Clones
gRNAs

Summary

Gene Symbol	gclc
Entrez Gene ID	103179004
Full Name	glutamate-cysteine ligase catalytic subunit
Gene Type	protein-coding
Organism	Callorhinchus milii(elephant shark)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007894053.1	XP_007892244.1	glutamate--cysteine ligase catalytic subunit

Pathways from BioSystems
Gene Ontology

The following gclc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gclc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa376597	XM_007894053.1 Latest version!	Callorhinchus milii glutamate-cysteine ligase, catalytic subunit (gclc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gclc ( XM_007894053.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007892244.1 Callorhinchus milii glutamate-cysteine ligase, catalytic subunit (gclc), mRNA.

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CloneID	OCa376597
Clone ID Related Accession (Same CDS sequence)	XM_007894053.1
Accession Version	XM_007894053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	glutamate--cysteine ligase catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCTCC TGTCGGAAGG TTCGCCGTTG AACTGGGAAG AGACCAAGAA ATACGCCGAC 
CACGTCCGCA AACACGGCAT CATCCAGTTC CTCAACATCT ACCACAAAGT CAAGGACCGT 
CAGAAGGATG TCCTCAAATG GGGCGACGAG GTTGAGTATA TGTTGTTATC ATTTGATCAT 
AAACGTCAGA GAGTTCAAGT GTTGCTTCGG GGAGAAGAGA TTCTTCATAT ACTACAAGAC 
CAGGGCGAAA AAGCGAATCC TAACCATCCC ACACTTTGGA GACCTGAATA TGGCAGCTAT 
ATGATTGAAG GGACACCAGG ACAACCGTAT GGGGGAACTA TGGCTGATTT TAACACTGTG 
GAAGCAAACA TGAAATTACG TCGACAAGAA GTATTTTCAC TTTTGAAAGA TAATGAGACC 
ATATGCAGCA TCACAGCTTT CCCCAGGTTG GGATGTCCGG GGTTCACACA ACCTGAATAC 
GATCCAAAGC CACTTGAATG GGGAGCATCC AAATCATTGT TCTTTCCTGA TGAAGGAATT 
AATAAACATC CAAGATTCAG CACTTTGACT AGGAATATCC GTCACAGGCG AGGAGAGAAA 
ATAGCTATTA ATGTACCAAT CTATAAAGAT AAAAATACTC CTTCTCCATT CATTGAAAAA 
TTTATTAATG ATGATGGAGA GGCAGCAAGA GAAGCAAAGA CAGACCATAT CTATATGGAT 
GCTATGGGTT TCGGCATGGG CAATTGCTGT CTTCAGGTAA CATTTCAGGC ATGCAGCATC 
TCCGAAGCCA GGTATCTCTA TGACCAGCTC GCGACAATTT GCCCTATTGT GATGGCCTTG 
AGTGCTGCCT CGCCAATTTA TAGAGGTTAT GTGTCTGACA TTGACTGTCG TTGGGGAATA 
ATTTCTGCCT CTGTCGACGA CCGGACGCGA GAAGAAAGAG GTTTAGAGCC TTGTAAGTAC 
AGTAAGTACA GAATCAGCAA ATCAAGATAC GACTCCATTG ATAGTTACCT CTCAAAATGT 
GGTGAGAAAT ACAATGACAT AGAAGTAACA ATTGATGAAG AAATCTACCA TCAGCTTCGA 
GATGGAGGTA TTGATCATCT CCTGGCACAG CATCTAGCTC ATCTTTTTAT TCGGGATCCT 
TTGACACTGT ATGCAGAAAA GATACACTTG GATGATGCAA ATGAGTCTGA CCACTTTGAG 
AATATACAGT CCACTAATTG GCAAACTATG AGATTCAAAC CACCTCCACC AAACTCTGAA 
ATTGGATGGA GAGTTGAATT TCGACCTATG GAAGTGCAGT TAACAGATTT TGAGAATTCT 
GCATACGTGG TCTTTGTGGT ATTACTGACG AGAGTGATTC TTTCATACAA ACTGGATTTC 
CTCATTCCAC TCTCAAAGGT GGATGAAAAT TTGAAAGTGG CTCAGAAGCG TGATGCTGTA 
CTCCAAGGAT TGTTCTACTT CAGAAAAGAA ATCCATAAAA CTGGAAACCC AGTTGTCGAT 
GGCTGTAGCA GTTCACACAA TGGGATTGAT TCAGATACAG AAGATTACAC ATTAATGACC 
ATAGATACAA TCATTAATGG AAAGGAAGGT GTTTTTCCAG GTTTAATCCC AATTATAAAC 
TGTTATCTCG AAAACATGGA AGTAGATGTG GAAACAAGAT GCAGCATTTT GAACTACCTT 
AAGCTGATCA GAAAGCGAGC TTCGGGAGCG CTGATGACTG CAGCGTGTTG GTTGAGGAAG 
TTTGTTGCTA ATCACCCTAA CTACAAGCAA GATAGTGTTG TAAATGAAAA AATCAACTTT 
GACCTGATTA CGAAATGTGA CCAAATTGCA AAAGAGATAT GTAACTGCCC AGAACTGATT 
GGAGAACCTG TCAGCAAGGC CAAACATTCA GAAGGCATGA CTGGCGCTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007892244.1
CDS	164..2077
Translation	MGLLSEGSPLNWEETKKYADHVRKHGIIQFLNIYHKVKDRQKDVLKWGDEVEYMLLSFDHKRQRVQVLLRGEEILHILQDQGEKANPNHPTLWRPEYGSYMIEGTPGQPYGGTMADFNTVEANMKLRRQEVFSLLKDNETICSITAFPRLGCPGFTQPEYDPKPLEWGASKSLFFPDEGINKHPRFSTLTRNIRHRRGEKIAINVPIYKDKNTPSPFIEKFINDDGEAAREAKTDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPIYRGYVSDIDCRWGIISASVDDRTREERGLEPCKYSKYRISKSRYDSIDSYLSKCGEKYNDIEVTIDEEIYHQLRDGGIDHLLAQHLAHLFIRDPLTLYAEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSEIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENLKVAQKRDAVLQGLFYFRKEIHKTGNPVVDGCSSSHNGIDSDTEDYTLMTIDTIINGKEGVFPGLIPIINCYLENMEVDVETRCSILNYLKLIRKRASGALMTAACWLRKFVANHPNYKQDSVVNEKINFDLITKCDQIAKEICNCPELIGEPVSKAKHSEGMTGAV

Target ORF information:

RefSeq Version	XM_007894053.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii glutamate-cysteine ligase, catalytic subunit (gclc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007894053.1

ORF Insert Sequence:

ATGGGTCTCC TGTCGGAAGG TTCGCCGTTG AACTGGGAAG AGACCAAGAA ATACGCCGAC 
CACGTCCGCA AACACGGCAT CATCCAGTTC CTCAACATCT ACCACAAAGT CAAGGACCGT 
CAGAAGGATG TCCTCAAATG GGGCGACGAG GTTGAGTATA TGTTGTTATC ATTTGATCAT 
AAACGTCAGA GAGTTCAAGT GTTGCTTCGG GGAGAAGAGA TTCTTCATAT ACTACAAGAC 
CAGGGCGAAA AAGCGAATCC TAACCATCCC ACACTTTGGA GACCTGAATA TGGCAGCTAT 
ATGATTGAAG GGACACCAGG ACAACCGTAT GGGGGAACTA TGGCTGATTT TAACACTGTG 
GAAGCAAACA TGAAATTACG TCGACAAGAA GTATTTTCAC TTTTGAAAGA TAATGAGACC 
ATATGCAGCA TCACAGCTTT CCCCAGGTTG GGATGTCCGG GGTTCACACA ACCTGAATAC 
GATCCAAAGC CACTTGAATG GGGAGCATCC AAATCATTGT TCTTTCCTGA TGAAGGAATT 
AATAAACATC CAAGATTCAG CACTTTGACT AGGAATATCC GTCACAGGCG AGGAGAGAAA 
ATAGCTATTA ATGTACCAAT CTATAAAGAT AAAAATACTC CTTCTCCATT CATTGAAAAA 
TTTATTAATG ATGATGGAGA GGCAGCAAGA GAAGCAAAGA CAGACCATAT CTATATGGAT 
GCTATGGGTT TCGGCATGGG CAATTGCTGT CTTCAGGTAA CATTTCAGGC ATGCAGCATC 
TCCGAAGCCA GGTATCTCTA TGACCAGCTC GCGACAATTT GCCCTATTGT GATGGCCTTG 
AGTGCTGCCT CGCCAATTTA TAGAGGTTAT GTGTCTGACA TTGACTGTCG TTGGGGAATA 
ATTTCTGCCT CTGTCGACGA CCGGACGCGA GAAGAAAGAG GTTTAGAGCC TTGTAAGTAC 
AGTAAGTACA GAATCAGCAA ATCAAGATAC GACTCCATTG ATAGTTACCT CTCAAAATGT 
GGTGAGAAAT ACAATGACAT AGAAGTAACA ATTGATGAAG AAATCTACCA TCAGCTTCGA 
GATGGAGGTA TTGATCATCT CCTGGCACAG CATCTAGCTC ATCTTTTTAT TCGGGATCCT 
TTGACACTGT ATGCAGAAAA GATACACTTG GATGATGCAA ATGAGTCTGA CCACTTTGAG 
AATATACAGT CCACTAATTG GCAAACTATG AGATTCAAAC CACCTCCACC AAACTCTGAA 
ATTGGATGGA GAGTTGAATT TCGACCTATG GAAGTGCAGT TAACAGATTT TGAGAATTCT 
GCATACGTGG TCTTTGTGGT ATTACTGACG AGAGTGATTC TTTCATACAA ACTGGATTTC 
CTCATTCCAC TCTCAAAGGT GGATGAAAAT TTGAAAGTGG CTCAGAAGCG TGATGCTGTA 
CTCCAAGGAT TGTTCTACTT CAGAAAAGAA ATCCATAAAA CTGGAAACCC AGTTGTCGAT 
GGCTGTAGCA GTTCACACAA TGGGATTGAT TCAGATACAG AAGATTACAC ATTAATGACC 
ATAGATACAA TCATTAATGG AAAGGAAGGT GTTTTTCCAG GTTTAATCCC AATTATAAAC 
TGTTATCTCG AAAACATGGA AGTAGATGTG GAAACAAGAT GCAGCATTTT GAACTACCTT 
AAGCTGATCA GAAAGCGAGC TTCGGGAGCG CTGATGACTG CAGCGTGTTG GTTGAGGAAG 
TTTGTTGCTA ATCACCCTAA CTACAAGCAA GATAGTGTTG TAAATGAAAA AATCAACTTT 
GACCTGATTA CGAAATGTGA CCAAATTGCA AAAGAGATAT GTAACTGCCC AGAACTGATT 
GGAGAACCTG TCAGCAAGGC CAAACATTCA GAAGGCATGA CTGGCGCTGT TTAA