NPL cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	NPL
Entrez Gene ID	104502235
Full Name	N-acetylneuraminate pyruvate lyase
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010146181.1	XP_010144483.1	LOW QUALITY PROTEIN: N-acetylneuraminate lyase

Pathways from BioSystems
Gene Ontology

The following NPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu35299	XM_010146181.1 Latest version!	Buceros rhinoceros silvestris N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPL ( XM_010146181.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010144483.1 Buceros rhinoceros silvestris N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), mRNA.

Price & Availability↑

CloneID	OBu35299
Clone ID Related Accession (Same CDS sequence)	XM_010146181.1
Accession Version	XM_010146181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	LOW QUALITY PROTEIN: N-acetylneuraminate lyase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010397225.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGACACCCG GAAAGAAGTT AGAGGGTCTT GTAGCNGCTA CAATCACTCC AATGTCTCCT 
GATGGACAAA TTAACCTCCC GGTGATTGGT CAATATGTGG ATTATCTGGT GAGCGAGCAG 
AACGTGAAGA ACGTCTTTGT GAATGGCACA ACAGGAGAAG GACTGTCCCT TAACACCCAG 
GAGAGGAAGC AGTTAGCGGA AGAATGGGTG TGCCAAGGAA AAAACAAGTT GGATCACGTG 
ATCGTTCATG TGGGAGCACT GAGTCTGGCA GAGTCTCAAG AGCTGGCAAG ACACGCAGCA 
GCCATAGGCG CTAGTGCTAT TGCTGTCATA GCCCCCTTCT TCTTCAGACC CATAAACAAA 
GATGAGCTGA TTGCTTTCCT ACAGAAGGTT GCGTCTGAAG CCCCTACGGT TCCATTTTAT 
TACTATCACA TTCCTCATCT GACGGGCGTG AAGATTCGTG TTGAAGAGTT GCTGGATGGA 
ATAAAAGAGC GGATCCCCAC CTTCCAGGGT GTGAAGTTCA GTGGCACGGA CCTCCTGGAC 
CTTGCACAGT GTATAAACAA GACTGAGAGA GAACAGTATG CATTTCTTTA TGGGGTGGAT 
GAGCAACTGT TGAGTGCGCT GGCAATAGGG GCAGATGGAG CAGTTGGAAG TACCTACAAC 
TATTTGGGTC AAAAAACCAA TCTGATGTTG CAAGCCTTTG CAAAGCCAGA CCTTCTGTTA 
GCACGGAAGT ATCAGTTTCT CACCGGGGAA TTTCTCAACT TTGTCATAAA ACTAGGTTTC 
GGTGTTGCAG AGACCAAAGC TGTAATGACT TTCGTTTCTG GCATTCCCAT GGGACCTCCA 
CGGCTTCCCC TTGTTGATGC CTCCGAGGAG TTCATCGTCA AGGCCAAAGC CAAGCTGGAT 
AGCATTGTGT GGCCTGATGG TGACTGACAT GCGCTTCCAT GTCAATCGGG AAGTGGAGGC 
TCCTTTTCTG GTCATTGCTG TCACGTGGCA CGTTCCCCAC ATGCTGAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010144483.1
CDS	1..1014
Translation	MTPGKKLEGLVnullATITPMSPDGQINLPVIGQYVDYLVSEQNVKNVFVNGTTGEGLSLNTQERKQLAEEWVCQGKNKLDHVIVHVGALSLAESQELARHAAAIGASAIAVIAPFFFRPINKDELIAFLQKVASEAPTVPFYYYHIPHLTGVKIRVEELLDGIKERIPTFQGVKFSGTDLLDLAQCINKTEREQYAFLYGVDEQLLSALAIGADGAVGSTYNYLGQKTNLMLQAFAKPDLLLARKYQFLTGEFLNFVIKLGFGVAETKAVMTFVSGIPMGPPRLPLVDASEEFIVKAKAKLDSIVWPDGD*HALPCQSGSGGSFSGHCCHVARSPHAEL

Target ORF information:

RefSeq Version	XM_010146181.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010146181.1

ORF Insert Sequence:

ATGACACCCG GAAAGAAGTT AGAGGGTCTT GTAGCNGCTA CAATCACTCC AATGTCTCCT 
GATGGACAAA TTAACCTCCC GGTGATTGGT CAATATGTGG ATTATCTGGT GAGCGAGCAG 
AACGTGAAGA ACGTCTTTGT GAATGGCACA ACAGGAGAAG GACTGTCCCT TAACACCCAG 
GAGAGGAAGC AGTTAGCGGA AGAATGGGTG TGCCAAGGAA AAAACAAGTT GGATCACGTG 
ATCGTTCATG TGGGAGCACT GAGTCTGGCA GAGTCTCAAG AGCTGGCAAG ACACGCAGCA 
GCCATAGGCG CTAGTGCTAT TGCTGTCATA GCCCCCTTCT TCTTCAGACC CATAAACAAA 
GATGAGCTGA TTGCTTTCCT ACAGAAGGTT GCGTCTGAAG CCCCTACGGT TCCATTTTAT 
TACTATCACA TTCCTCATCT GACGGGCGTG AAGATTCGTG TTGAAGAGTT GCTGGATGGA 
ATAAAAGAGC GGATCCCCAC CTTCCAGGGT GTGAAGTTCA GTGGCACGGA CCTCCTGGAC 
CTTGCACAGT GTATAAACAA GACTGAGAGA GAACAGTATG CATTTCTTTA TGGGGTGGAT 
GAGCAACTGT TGAGTGCGCT GGCAATAGGG GCAGATGGAG CAGTTGGAAG TACCTACAAC 
TATTTGGGTC AAAAAACCAA TCTGATGTTG CAAGCCTTTG CAAAGCCAGA CCTTCTGTTA 
GCACGGAAGT ATCAGTTTCT CACCGGGGAA TTTCTCAACT TTGTCATAAA ACTAGGTTTC 
GGTGTTGCAG AGACCAAAGC TGTAATGACT TTCGTTTCTG GCATTCCCAT GGGACCTCCA 
CGGCTTCCCC TTGTTGATGC CTCCGAGGAG TTCATCGTCA AGGCCAAAGC CAAGCTGGAT 
AGCATTGTGT GGCCTGATGG TGACTGACAT GCGCTTCCAT GTCAATCGGG AAGTGGAGGC 
TCCTTTTCTG GTCATTGCTG TCACGTGGCA CGTTCCCCAC ATGCTGAGCT GTGA