MED12L cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	MED12L
Entrez Gene ID	104499652
Full Name	mediator complex subunit 12 like
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010143182.1	XP_010141484.1	mediator of RNA polymerase II transcription subunit 12-like protein

Pathways from BioSystems
Gene Ontology

The following MED12L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED12L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu40947	XM_010143182.1 Latest version!	Buceros rhinoceros silvestris mediator complex subunit 12-like (MED12L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED12L ( XM_010143182.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010141484.1 Buceros rhinoceros silvestris mediator complex subunit 12-like (MED12L), mRNA.

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CloneID	OBu40947
Clone ID Related Accession (Same CDS sequence)	XM_010143182.1
Accession Version	XM_010143182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	mediator of RNA polymerase II transcription subunit 12-like protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010430899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGATT TCATCGAGAA CCCTTCTGCT CACAACATTA ACTACTCAAT GCTGGGCAAG 
ATCCTGAGCG ATAACGCGGC CAACCGCTAC AGCTTCGTCT GCAACGCGCT GATGAACGTG 
TGCATGGGGC ACCAGGACAC TGGGAGGATT AATGACATAG CAAACCTTTG CGCAGAGCTG 
ACGGCATGCT GCACGGTGCT TAGCTCAGAG TGGGTAGGGG TCGTAAAAGC TCTTTGCTGC 
TCTTCAAATC ATGTCTGGGG TTTCAATGAC CTGCTCTGCA GTGTGGATGT GAGTGACCTG 
TCGTTCCACG ATTCCCTGGC CACTTTCATT GCCATACTGA TCGCCCGGCA GTGCTTTTCT 
CTGGAAGACG TGGTTCAGCA TGTTGCACTG CCTTCTCTTC TTGCAGCTGC CTGTGGAGAT 
CCAGATGCCG AGCCTGGGGC GCGGATGACC TGCCGGCTGC TCCTGCACCT CTTCAGGACA 
CCCCAGGTCT GCCTCTTTCC CCAGGGAGCA GGGAAATTAT TTCCTGGAAT CCGTTCCTCT 
TGCGATCGTC ACCTCTTGGC TGCTGCTCAC AACAGTATAG AAGTGGGAGC CGTGTTTGCA 
GTCTTAAAGG CAATTTTGAT GCTTGGTGAT GCCGGAATTG GCAGCAGCAG CGTCAGCTCC 
TTGAAGAGTG AAGACTTCCA TGTGAGAGGC TTGTTAGATG ACCTCAGTGA GGATGAAATC 
TGGGGCTCCT CTCACACTTT GAAATTGTGC GGAAAAGCTG TTTCCATAGA AACTGCAAGT 
TTAAGCGAGT ATGCTAGATA TGTGCTAAGA ACAATTTGCC AGCAGGAATG GGTTGGAGAG 
CACTGTCTGA AAGAACCCGA GAGGCTGTGC ACAGACAAAG ATCTGATTTT GGATCCTGTT 
CTTTCAAACA AACAAGCACA GAAACTCCTT CAACTTATCT GTTATCCTCA TGGTATCAAG 
GAATGCTCCG AGGGTGACAG CCCTCAGAGG CAGCACATCA AACGCATTCT TCAGAACTTG 
GACCAGTGGA CTCTCCGGCA GTCTTGGTTG GAGCTGCAGC TAATGATCAA ACAGTGCATG 
AAGGAGCCTT TCCTCCGAGC GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT 
GCCTACTTCA GTTCAAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010141484.1
CDS	554..1714
Translation	MLDFIENPSAHNINYSMLGKILSDNAANRYSFVCNALMNVCMGHQDTGRINDIANLCAELTACCTVLSSEWVGVVKALCCSSNHVWGFNDLLCSVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDPDAEPGARMTCRLLLHLFRTPQVCLFPQGAGKLFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAILMLGDAGIGSSSVSSLKSEDFHVRGLLDDLSEDEIWGSSHTLKLCGKAVSIETASLSEYARYVLRTICQQEWVGEHCLKEPERLCTDKDLILDPVLSNKQAQKLLQLICYPHGIKECSEGDSPQRQHIKRILQNLDQWTLRQSWLELQLMIKQCMKEPFLRAARSVAGGSLDCKTAYFSSR

Target ORF information:

RefSeq Version	XM_010143182.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris mediator complex subunit 12-like (MED12L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010143182.1

ORF Insert Sequence:

ATGCTCGATT TCATCGAGAA CCCTTCTGCT CACAACATTA ACTACTCAAT GCTGGGCAAG 
ATCCTGAGCG ATAACGCGGC CAACCGCTAC AGCTTCGTCT GCAACGCGCT GATGAACGTG 
TGCATGGGGC ACCAGGACAC TGGGAGGATT AATGACATAG CAAACCTTTG CGCAGAGCTG 
ACGGCATGCT GCACGGTGCT TAGCTCAGAG TGGGTAGGGG TCGTAAAAGC TCTTTGCTGC 
TCTTCAAATC ATGTCTGGGG TTTCAATGAC CTGCTCTGCA GTGTGGATGT GAGTGACCTG 
TCGTTCCACG ATTCCCTGGC CACTTTCATT GCCATACTGA TCGCCCGGCA GTGCTTTTCT 
CTGGAAGACG TGGTTCAGCA TGTTGCACTG CCTTCTCTTC TTGCAGCTGC CTGTGGAGAT 
CCAGATGCCG AGCCTGGGGC GCGGATGACC TGCCGGCTGC TCCTGCACCT CTTCAGGACA 
CCCCAGGTCT GCCTCTTTCC CCAGGGAGCA GGGAAATTAT TTCCTGGAAT CCGTTCCTCT 
TGCGATCGTC ACCTCTTGGC TGCTGCTCAC AACAGTATAG AAGTGGGAGC CGTGTTTGCA 
GTCTTAAAGG CAATTTTGAT GCTTGGTGAT GCCGGAATTG GCAGCAGCAG CGTCAGCTCC 
TTGAAGAGTG AAGACTTCCA TGTGAGAGGC TTGTTAGATG ACCTCAGTGA GGATGAAATC 
TGGGGCTCCT CTCACACTTT GAAATTGTGC GGAAAAGCTG TTTCCATAGA AACTGCAAGT 
TTAAGCGAGT ATGCTAGATA TGTGCTAAGA ACAATTTGCC AGCAGGAATG GGTTGGAGAG 
CACTGTCTGA AAGAACCCGA GAGGCTGTGC ACAGACAAAG ATCTGATTTT GGATCCTGTT 
CTTTCAAACA AACAAGCACA GAAACTCCTT CAACTTATCT GTTATCCTCA TGGTATCAAG 
GAATGCTCCG AGGGTGACAG CCCTCAGAGG CAGCACATCA AACGCATTCT TCAGAACTTG 
GACCAGTGGA CTCTCCGGCA GTCTTGGTTG GAGCTGCAGC TAATGATCAA ACAGTGCATG 
AAGGAGCCTT TCCTCCGAGC GGCGAGGAGT GTGGCTGGTG GCTCCCTTGA TTGCAAAACT 
GCCTACTTCA GTTCAAGGTA G