MME cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	MME
Entrez Gene ID	104499547
Full Name	membrane metalloendopeptidase
Synonyms	Neprilysin
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010143064.1	XP_010141366.1	neprilysin

Pathways from BioSystems
Gene Ontology

The following MME gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MME cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu40890	XM_010143064.1 Latest version!	Buceros rhinoceros silvestris membrane metallo-endopeptidase (MME), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MME ( XM_010143064.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010141366.1 Buceros rhinoceros silvestris membrane metallo-endopeptidase (MME), mRNA.

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CloneID	OBu40890
Clone ID Related Accession (Same CDS sequence)	XM_010143064.1
Accession Version	XM_010143064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	neprilysin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010430517.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAGT CGGAAAGCCA GATGGACATA ACTGAGATGA ACACCCCAAA GCCAAAGAAG 
AAACTGCGCT GCAGTGCCCT GGAGATCGGG CTGGCTGTGG TAGTGATTCT GCTGGCAATA 
GTCGCGATCA CCATGATCGT TCTGTATGCC ACCTATGACG ATGGGGTTTG CAAGACATCA 
GACTGCATAA AATCAGCAGC CCGAATCCTT GAGAACATGG ACACCAGTGC AGAGCCCTGC 
AAGGATTTCT ACCAATATGC CTGTGGAGGA TGGCTGAAGA GGAACGTCAT TCCAGAAACC 
AGCTCCCGTT ATAGTAACTT TGACATTTTA AGAGACGAAC TAGAAGTTGT TTTAAAAGAT 
GTCCTTGACA CACCGAGCAC TAACGACATA GCAGCCGTGC AGAAGGCAAA AACTCTGTAC 
AGATCGTGCA TTAATGAAAC TACCATTGAT AGCAGAGGTG GGAGGCCTCT AATCAGTTTA 
CTGCCCAGTG TATCTGACTG GCCTGTAGCA ACAACTAACT GGGAATCGTC CTATGGTCCC 
ACTTGGACAG CGGAGACAGC CATTGCACAG CTCAACTCCA GATACGGAAA AAAGGTCCTG 
ATTAATTTTT TTGTGGGCAC AGATGATAAA AATTCCACGG CACATATCAT GCACATTGAC 
CAGCCCGGGC TTGGCCTGCC TTCTCGGGAC TACTACGAGT GCACTGGTGT ATACAAAGAG 
GCATGTTCTG TCTATGTCGA TTTCATGATT TCTGTAGCTA AATTAATCCT ACAAGAAAGA 
AATATTTCTT TCAATGAGAG CGAAATTTCT GAACAGATGA GAAGAATTAT GGACCTGGAG 
AAGGAGATCG CCAATGCAAC AACAAGATCT GAAGACAGGA ATGATCCACT TTTGCTGTAC 
AATAAAATGA CCTTGGCTGA CCTCCAAAAG AACTTCTCAC TGGAGTTCAA TCATAAGGTA 
TTCAACTGGT CAAAATTCAT AAATGAGATA ATGTCCACTG TACAAATTAA CATCGAGAAC 
ACGGAGCATG TCATCGTTTA TGATCCAGAA TACCTCCTCA GGCTGAAGCC TATTCTGACT 
AACTACACTG CCAGAGACTT TCAGAATTAC ATAATCTGGC GCTTCATAAT GGATCTTGTC 
AACAGCCTGA GCCGTAACTA CAAGGATACA AGGAACGCTT TTCTTAAGGC ACTTTATGGC 
ACAACATCAG AAACTGCTGT TTGGCGGCGC TGCGCAAACT ACGTCAATGG CAAGATGGAG 
AATGCGGTGG GACGGTTATA TGTCAAGGAA GCATTTGCTG GAGACAGCAA ACATGTGGTG 
GAGGAAATGA TTGCAGACAT ACGTGATGTT TTTATAAAAA CCCTAGATGA ACTTACCTGG 
ATGGATGCAG AAACCAAAAA GAAAGCAGAG CAGAAAGCCA CAGCAATCAG AGAGAGGATT 
GGCTACCCCG ACGAGATCCT GACAGACGAC AGTAAGCTGA ACAGCGAGTA CCAGGAGTTG 
AACTTCAAAG AAGAAGAATA CTTTGAAAAT ATTATTCAAA ATTTAATATT TACCCAGAAG 
AAAAGGTTGA AAAAGCTTAG AGAAACGGTC GACAAAGAGG AGTGGATAAG TGGAGCTGCT 
GTGGTCAATG CGTTTTATTC AGCGAGCAGA AATCAGATAG TGTTCCCTGC CGGCATTTTG 
CAGCCCCCTT TCTTCAGTGC GTCCCAGCCC AAATCTCTGA ATTACGGAGG CATAGGCATG 
GTCATCGGGC ATGAGATCAC CCACGGGTTT GACGACAACG GCAGGAATTT TAATGAGAAT 
GGAGACCTTG TGGACTGGTG GAGCGAAGCA TCGGCACGCA ATTTTAAGGA GCTCTCCCAG 
TGCATGGTGT ACCAGTACGG GAACTTCTCG TGGGACCTGG CAGGTGGACA GCACCTGAGC 
GGAATCAACA CGCTGGGAGA AAACATTGCT GATAACGGGG GGGTCAGACA AGCGTATAAG 
GCCTATGAAA ACTTTGTGCA AAAGCATGGG AAAGAAAAAC TTCTTCCTGG TCTTGACATG 
AACCACCAGC AGCTGTTTTT CCTGAACTTC GCACAGATAT GGTGTGGAAC ATACAGACCA 
GAGTATGCCG TGAACTCCAT CAAAACTGAT GTGCACAGCC CAGGAAAATT CAGGGTCATC 
GGATCACTGC AGAACTCTCC GGAGTTTTCT GAAGCCTTTT CATGCACCAA GACAAGCTAC 
ATGGATCCTG TTAAGAAGTG CCGCGTTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010141366.1
CDS	4..2256
Translation	MGKSESQMDITEMNTPKPKKKLRCSALEIGLAVVVILLAIVAITMIVLYATYDDGVCKTSDCIKSAARILENMDTSAEPCKDFYQYACGGWLKRNVIPETSSRYSNFDILRDELEVVLKDVLDTPSTNDIAAVQKAKTLYRSCINETTIDSRGGRPLISLLPSVSDWPVATTNWESSYGPTWTAETAIAQLNSRYGKKVLINFFVGTDDKNSTAHIMHIDQPGLGLPSRDYYECTGVYKEACSVYVDFMISVAKLILQERNISFNESEISEQMRRIMDLEKEIANATTRSEDRNDPLLLYNKMTLADLQKNFSLEFNHKVFNWSKFINEIMSTVQINIENTEHVIVYDPEYLLRLKPILTNYTARDFQNYIIWRFIMDLVNSLSRNYKDTRNAFLKALYGTTSETAVWRRCANYVNGKMENAVGRLYVKEAFAGDSKHVVEEMIADIRDVFIKTLDELTWMDAETKKKAEQKATAIRERIGYPDEILTDDSKLNSEYQELNFKEEEYFENIIQNLIFTQKKRLKKLRETVDKEEWISGAAVVNAFYSASRNQIVFPAGILQPPFFSASQPKSLNYGGIGMVIGHEITHGFDDNGRNFNENGDLVDWWSEASARNFKELSQCMVYQYGNFSWDLAGGQHLSGINTLGENIADNGGVRQAYKAYENFVQKHGKEKLLPGLDMNHQQLFFLNFAQIWCGTYRPEYAVNSIKTDVHSPGKFRVIGSLQNSPEFSEAFSCTKTSYMDPVKKCRVW

Target ORF information:

RefSeq Version	XM_010143064.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris membrane metallo-endopeptidase (MME), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010143064.1

ORF Insert Sequence:

ATGGGCAAGT CGGAAAGCCA GATGGACATA ACTGAGATGA ACACCCCAAA GCCAAAGAAG 
AAACTGCGCT GCAGTGCCCT GGAGATCGGG CTGGCTGTGG TAGTGATTCT GCTGGCAATA 
GTCGCGATCA CCATGATCGT TCTGTATGCC ACCTATGACG ATGGGGTTTG CAAGACATCA 
GACTGCATAA AATCAGCAGC CCGAATCCTT GAGAACATGG ACACCAGTGC AGAGCCCTGC 
AAGGATTTCT ACCAATATGC CTGTGGAGGA TGGCTGAAGA GGAACGTCAT TCCAGAAACC 
AGCTCCCGTT ATAGTAACTT TGACATTTTA AGAGACGAAC TAGAAGTTGT TTTAAAAGAT 
GTCCTTGACA CACCGAGCAC TAACGACATA GCAGCCGTGC AGAAGGCAAA AACTCTGTAC 
AGATCGTGCA TTAATGAAAC TACCATTGAT AGCAGAGGTG GGAGGCCTCT AATCAGTTTA 
CTGCCCAGTG TATCTGACTG GCCTGTAGCA ACAACTAACT GGGAATCGTC CTATGGTCCC 
ACTTGGACAG CGGAGACAGC CATTGCACAG CTCAACTCCA GATACGGAAA AAAGGTCCTG 
ATTAATTTTT TTGTGGGCAC AGATGATAAA AATTCCACGG CACATATCAT GCACATTGAC 
CAGCCCGGGC TTGGCCTGCC TTCTCGGGAC TACTACGAGT GCACTGGTGT ATACAAAGAG 
GCATGTTCTG TCTATGTCGA TTTCATGATT TCTGTAGCTA AATTAATCCT ACAAGAAAGA 
AATATTTCTT TCAATGAGAG CGAAATTTCT GAACAGATGA GAAGAATTAT GGACCTGGAG 
AAGGAGATCG CCAATGCAAC AACAAGATCT GAAGACAGGA ATGATCCACT TTTGCTGTAC 
AATAAAATGA CCTTGGCTGA CCTCCAAAAG AACTTCTCAC TGGAGTTCAA TCATAAGGTA 
TTCAACTGGT CAAAATTCAT AAATGAGATA ATGTCCACTG TACAAATTAA CATCGAGAAC 
ACGGAGCATG TCATCGTTTA TGATCCAGAA TACCTCCTCA GGCTGAAGCC TATTCTGACT 
AACTACACTG CCAGAGACTT TCAGAATTAC ATAATCTGGC GCTTCATAAT GGATCTTGTC 
AACAGCCTGA GCCGTAACTA CAAGGATACA AGGAACGCTT TTCTTAAGGC ACTTTATGGC 
ACAACATCAG AAACTGCTGT TTGGCGGCGC TGCGCAAACT ACGTCAATGG CAAGATGGAG 
AATGCGGTGG GACGGTTATA TGTCAAGGAA GCATTTGCTG GAGACAGCAA ACATGTGGTG 
GAGGAAATGA TTGCAGACAT ACGTGATGTT TTTATAAAAA CCCTAGATGA ACTTACCTGG 
ATGGATGCAG AAACCAAAAA GAAAGCAGAG CAGAAAGCCA CAGCAATCAG AGAGAGGATT 
GGCTACCCCG ACGAGATCCT GACAGACGAC AGTAAGCTGA ACAGCGAGTA CCAGGAGTTG 
AACTTCAAAG AAGAAGAATA CTTTGAAAAT ATTATTCAAA ATTTAATATT TACCCAGAAG 
AAAAGGTTGA AAAAGCTTAG AGAAACGGTC GACAAAGAGG AGTGGATAAG TGGAGCTGCT 
GTGGTCAATG CGTTTTATTC AGCGAGCAGA AATCAGATAG TGTTCCCTGC CGGCATTTTG 
CAGCCCCCTT TCTTCAGTGC GTCCCAGCCC AAATCTCTGA ATTACGGAGG CATAGGCATG 
GTCATCGGGC ATGAGATCAC CCACGGGTTT GACGACAACG GCAGGAATTT TAATGAGAAT 
GGAGACCTTG TGGACTGGTG GAGCGAAGCA TCGGCACGCA ATTTTAAGGA GCTCTCCCAG 
TGCATGGTGT ACCAGTACGG GAACTTCTCG TGGGACCTGG CAGGTGGACA GCACCTGAGC 
GGAATCAACA CGCTGGGAGA AAACATTGCT GATAACGGGG GGGTCAGACA AGCGTATAAG 
GCCTATGAAA ACTTTGTGCA AAAGCATGGG AAAGAAAAAC TTCTTCCTGG TCTTGACATG 
AACCACCAGC AGCTGTTTTT CCTGAACTTC GCACAGATAT GGTGTGGAAC ATACAGACCA 
GAGTATGCCG TGAACTCCAT CAAAACTGAT GTGCACAGCC CAGGAAAATT CAGGGTCATC 
GGATCACTGC AGAACTCTCC GGAGTTTTCT GAAGCCTTTT CATGCACCAA GACAAGCTAC 
ATGGATCCTG TTAAGAAGTG CCGCGTTTGG TGA