RHOA cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	RHOA
Entrez Gene ID	104494849
Full Name	ras homolog family member A
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010137736.1	XP_010136038.1	transforming protein RhoA

Pathways from BioSystems
Gene Ontology

The following RHOA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHOA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu38497	XM_010137736.1 Latest version!	Buceros rhinoceros silvestris ras homolog family member A (RHOA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHOA ( XM_010137736.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010136038.1 Buceros rhinoceros silvestris ras homolog family member A (RHOA), mRNA.

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CloneID	OBu38497
Clone ID Related Accession (Same CDS sequence)	XM_010137736.1
Accession Version	XM_010137736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	transforming protein RhoA
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010416212.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA TTCGAAAAAA GCTGGTTATA GTGGGTGATG GTGCCTGTGG AAAGACTTGT 
CTGCTGATTG TATTTAGCAA AGACCAGTTC CCTGAAGTGT ATGTTCCCAC CGTCTTTGAA 
AATTATGTAG CAGATATTGA AGTGGATGGA AAGCAGGTTG AGTTGGCTTT GTGGGATACA 
GCAGGACAAG AAGACTATGA TCGACTTAGA CCGCTTTCTT ATCCAGATAC TGATGTTATA 
CTTATGTGTT TTTCAATTGA TAGTCCTGAT AGTTTAGAAA ACATCCCAGA GAAGTGGACC 
CCGGAGGTGA AGCATTTCTG CCCCAACGTG CCTATCATCT TGGTAGGAAA CAAGAAGGAC 
CTGAGGAACG ACGAGCACAC AAGACGAGAG CTGGCCAAAA TGAAGCAGGA GCCTGTCAAA 
CCCGAGGAAG GACGAGATAT GGCAAACCGC ATTGGTGCAT TCGGGTATAT GGAGTGTTCG 
GCAAAGACCA AAGACGGTGT GAGGGAGGTT TTTGAAATGG CCACTAGAGC TGCTTTGCAA 
GCCCGGCGTG GCAAGAAAAA GTCCGGGTGC CTTCTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010136038.1
CDS	6..587
Translation	MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLLL

Target ORF information:

RefSeq Version	XM_010137736.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris ras homolog family member A (RHOA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010137736.1

ORF Insert Sequence:

ATGGCAGCCA TTCGAAAAAA GCTGGTTATA GTGGGTGATG GTGCCTGTGG AAAGACTTGT 
CTGCTGATTG TATTTAGCAA AGACCAGTTC CCTGAAGTGT ATGTTCCCAC CGTCTTTGAA 
AATTATGTAG CAGATATTGA AGTGGATGGA AAGCAGGTTG AGTTGGCTTT GTGGGATACA 
GCAGGACAAG AAGACTATGA TCGACTTAGA CCGCTTTCTT ATCCAGATAC TGATGTTATA 
CTTATGTGTT TTTCAATTGA TAGTCCTGAT AGTTTAGAAA ACATCCCAGA GAAGTGGACC 
CCGGAGGTGA AGCATTTCTG CCCCAACGTG CCTATCATCT TGGTAGGAAA CAAGAAGGAC 
CTGAGGAACG ACGAGCACAC AAGACGAGAG CTGGCCAAAA TGAAGCAGGA GCCTGTCAAA 
CCCGAGGAAG GACGAGATAT GGCAAACCGC ATTGGTGCAT TCGGGTATAT GGAGTGTTCG 
GCAAAGACCA AAGACGGTGT GAGGGAGGTT TTTGAAATGG CCACTAGAGC TGCTTTGCAA 
GCCCGGCGTG GCAAGAAAAA GTCCGGGTGC CTTCTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.