NPHS2 cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	NPHS2
Entrez Gene ID	104493560
Full Name	NPHS2, podocin
Synonyms	Podocin
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010136270.1	XP_010134572.1	podocin

Pathways from BioSystems
Gene Ontology

The following NPHS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu37803	XM_010136270.1 Latest version!	Buceros rhinoceros silvestris nephrosis 2, idiopathic, steroid-resistant (podocin) (NPHS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPHS2 ( XM_010136270.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010134572.1 Buceros rhinoceros silvestris nephrosis 2, idiopathic, steroid-resistant (podocin) (NPHS2), mRNA.

Price & Availability↑

CloneID	OBu37803
Clone ID Related Accession (Same CDS sequence)	XM_010136270.1
Accession Version	XM_010136270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	podocin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010412154.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAGC GGTCTCGGAG CTCGTCCAGG GAGTCCCGCA GGAGACGCAG AGAGTCCCCA 
GCCACACAGA GCAAACGAGA GAAGAGGGAG AGCAGCAGAG AAGCTGGCAA AGGGAGGGGA 
GACATAAAGC AGGAGAAAGC AAAGGAGATC AAGAGCACCG CAGGGCCCGA CGGCAGGGTG 
CACACGTCCA CGGTGGTGGA CGTGGATGGT GTGATATCCG ATGAAGAGAT GGAGGCGATG 
GCCTTGCTGG ACAGCGAGCA GCCAGAGGAA GGTGTAAAGT CTCCCGGTCT GGGCATCTGT 
GAGTGGGTTC TGACCGTCCT GTCTACCCTG TTCGTCATAA TGACCTTCCC CATTTCTGTC 
TGGTTCTGCA TGAAGGTAGT GCGGGAGTAC GAGAGAGCCG TCGTGTTCCG GCTTGGGCAT 
CTCCTTCCAG GCAGACCGAG AGGGCCTGGC CTTTTCTTTT TGCTTCCCTG TCTGGACACT 
TATCACAAAG TAGACCTCCG CCTCAAAACC CTAGAGATCC CCTTCCATCA GGTGGTGACC 
AAAGACATGG TTACCTTGGA GATAGATGCT GTCTGCTACT ACCGACTGGA GAACGCCTCT 
CTTCTCCTCA CCACCCTGAC CAGCATCTCC AGTGCTATCC AGCTGCTGGT GCAGACCACC 
ACGAAGCGCC TCCTGGCACA CCGAGCCTTC TCTGAGCTTC TCTTGGAGAG GAAGAGCATC 
AGCCAGGAGA TAAAGGTGGC TTTGGACGCG GTCACGGGCT GCTGGGGGAT CAAGGTGGAG 
AGAACAGAAA TCAACAACGT GCAGCTGCCT GCTGAAGTCC GGCAGTCCCT GGCGGTGGAA 
GCGGAAGCCC AGAGACAGGC CAAAGCGCGG GTGATTGCTG CTGAGGCGGA AAAGGCTGCT 
TCTGAGTCGC TGCGGACGGC AGCCGAGATC CTGTCCAGTG CCCCTGCTGC CGCCCAGCTC 
CGTTACCTCC ACGCGCTGCA CTCCCTGACC GCAGAAAAAC CTCCTGCTTT CATCCTGCCC 
TTGCCTTTGG ATGCCACGAG CCTGGTCCCT CCGGCCACCC ACAGTCCTCC GGCAGCGAGC 
AGCCTCACAG ACACCACCAG GCTCCCAGAA AGCCCAAAGG ACAAGGACTC ACCCATGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010134572.1
CDS	1..1143
Translation	MDKRSRSSSRESRRRRRESPATQSKREKRESSREAGKGRGDIKQEKAKEIKSTAGPDGRVHTSTVVDVDGVISDEEMEAMALLDSEQPEEGVKSPGLGICEWVLTVLSTLFVIMTFPISVWFCMKVVREYERAVVFRLGHLLPGRPRGPGLFFLLPCLDTYHKVDLRLKTLEIPFHQVVTKDMVTLEIDAVCYYRLENASLLLTTLTSISSAIQLLVQTTTKRLLAHRAFSELLLERKSISQEIKVALDAVTGCWGIKVERTEINNVQLPAEVRQSLAVEAEAQRQAKARVIAAEAEKAASESLRTAAEILSSAPAAAQLRYLHALHSLTAEKPPAFILPLPLDATSLVPPATHSPPAASSLTDTTRLPESPKDKDSPML

Target ORF information:

RefSeq Version	XM_010136270.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris nephrosis 2, idiopathic, steroid-resistant (podocin) (NPHS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010136270.1

ORF Insert Sequence:

ATGGATAAGC GGTCTCGGAG CTCGTCCAGG GAGTCCCGCA GGAGACGCAG AGAGTCCCCA 
GCCACACAGA GCAAACGAGA GAAGAGGGAG AGCAGCAGAG AAGCTGGCAA AGGGAGGGGA 
GACATAAAGC AGGAGAAAGC AAAGGAGATC AAGAGCACCG CAGGGCCCGA CGGCAGGGTG 
CACACGTCCA CGGTGGTGGA CGTGGATGGT GTGATATCCG ATGAAGAGAT GGAGGCGATG 
GCCTTGCTGG ACAGCGAGCA GCCAGAGGAA GGTGTAAAGT CTCCCGGTCT GGGCATCTGT 
GAGTGGGTTC TGACCGTCCT GTCTACCCTG TTCGTCATAA TGACCTTCCC CATTTCTGTC 
TGGTTCTGCA TGAAGGTAGT GCGGGAGTAC GAGAGAGCCG TCGTGTTCCG GCTTGGGCAT 
CTCCTTCCAG GCAGACCGAG AGGGCCTGGC CTTTTCTTTT TGCTTCCCTG TCTGGACACT 
TATCACAAAG TAGACCTCCG CCTCAAAACC CTAGAGATCC CCTTCCATCA GGTGGTGACC 
AAAGACATGG TTACCTTGGA GATAGATGCT GTCTGCTACT ACCGACTGGA GAACGCCTCT 
CTTCTCCTCA CCACCCTGAC CAGCATCTCC AGTGCTATCC AGCTGCTGGT GCAGACCACC 
ACGAAGCGCC TCCTGGCACA CCGAGCCTTC TCTGAGCTTC TCTTGGAGAG GAAGAGCATC 
AGCCAGGAGA TAAAGGTGGC TTTGGACGCG GTCACGGGCT GCTGGGGGAT CAAGGTGGAG 
AGAACAGAAA TCAACAACGT GCAGCTGCCT GCTGAAGTCC GGCAGTCCCT GGCGGTGGAA 
GCGGAAGCCC AGAGACAGGC CAAAGCGCGG GTGATTGCTG CTGAGGCGGA AAAGGCTGCT 
TCTGAGTCGC TGCGGACGGC AGCCGAGATC CTGTCCAGTG CCCCTGCTGC CGCCCAGCTC 
CGTTACCTCC ACGCGCTGCA CTCCCTGACC GCAGAAAAAC CTCCTGCTTT CATCCTGCCC 
TTGCCTTTGG ATGCCACGAG CCTGGTCCCT CCGGCCACCC ACAGTCCTCC GGCAGCGAGC 
AGCCTCACAG ACACCACCAG GCTCCCAGAA AGCCCAAAGG ACAAGGACTC ACCCATGCTC 
TAA