THEMIS cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	THEMIS
Entrez Gene ID	104493180
Full Name	thymocyte selection associated
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010135813.1	XP_010134115.1	protein THEMIS

Pathways from BioSystems
Gene Ontology

The following THEMIS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THEMIS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu37589	XM_010135813.1 Latest version!	Buceros rhinoceros silvestris thymocyte selection associated (THEMIS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THEMIS ( XM_010135813.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010134115.1 Buceros rhinoceros silvestris thymocyte selection associated (THEMIS), mRNA.

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CloneID	OBu37589
Clone ID Related Accession (Same CDS sequence)	XM_010135813.1
Accession Version	XM_010135813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	protein THEMIS
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010411005.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGCATCTA CGCTGGAGAA GTTCATTCAT TCCCTGGACC CCAGAGCCCT TCCCAGGGTC 
CTCCAAATCC AGTCTGGCAT CTATTTCCAA GGCTCCATCT ATGAAATGTT TGGAAACGAA 
TGCTGCCTGT CAACAGGAGA AATCATTAAA GTTATTGGCT TCAAAATTAA TAAGCTTATA 
GCAAGTATCT GCGAGAATAA TGAAGTCAGC CAGCTTTCAG CCAAAGTGGA ATTGCCACTA 
AATTTTCCTG GTCTTTATAA GGTTGTGGCA GATAAAACTC CGTATGTCAG CATCGAAGAA 
ATTACAAGAA AAATCTCTAT TGGGCCCACA AGATTTGGCC ATCCATGTTT CTACAGCCCC 
GAGGATATAA AACTGGCAAA CCTCACTATC AAACGCGGTG AGCAGATCAC CTTCAACTCA 
GTGGAAGAGG TCAACGGAAC GATGGCTGTG AACTGTGGCG TGGTCAGAAA CAACCAGTCT 
CATTCCTTCA CCCTGCCCCT GTCGCAAGAG GGAAAATTCT ACGAGTGTGA AGATGACCAG 
ATATACACCC TCAAAGAGAT CGCAGAGTGG AAAATCCCCA AGTGCAGGAG CCGGATCGTC 
AAACTTTCCA ATATTTTACA CACGTGGGAC AGCTCTAATC CACTTCCTGA GAATTTTGAC 
GGCTGCTTGA CCCTCACGCC TGTCTATGAA GTGCAGGCAG TGATGAAATT TCGAAAGGAC 
ATAGTTCATA TCCTCTCAGA TCTGGATGTT GAGGTCAAGG ATATAACAGA CTGCTATGAT 
ATTAGCTCTT TCCTTCAGCC ACTGACTTTG GAAGATATTT TTGAAAGAAC AAGCAAGGAA 
TTTCCTATGG TTGCTGAGAT AATGGAAGGG CCTTCGGGAA GCCCAAAACC TTATAACTCA 
CTGTATACAG GAAAAGAGAT CGTCATCTAC AAAAAGTACC AGGCAACAAG AGTCCTAGCC 
TCGGAGATCA GAAGCGATTC CCCCAAAAGA CATTTTTTAA TCCCTATGAG CTACAAAGGA 
AAGTTCAAGA GAAGACCTCG GGAGTTTCCG ACTGCCTACG ACTTAGAGAT AGCAAGAAGC 
AAAGAGGAAC AGCTTCATGT TGTAGCAACT AAAGCCTTTG GTTCCCCTCA TGAAGAGCTT 
TTTTCTGTTT CTGTTGGAGA TCAATTTCTG GTTCAGCAAT GCCAGACCAG TGAAGTTCTG 
TACGAGGGAA GAAAGAAACT CGTAGATGTT TTGGCTTGTG AACAAATACT GAGTGATGCG 
TATAAGAAAG TGCTCGTCCC TATGTACATG GAAGGCGGCT TTGTAGAGGT GGTTCACGAC 
AAGAAACAGT ACCAGCTTTC TGAGATCTGC AAACGCTTTC GTCTGCCTTT TAATGTGAAA 
GTGGCCGTGA GGGACCTTTC CGTTGAGGAG GATGTCTTGG CTGCCGTGCC TGGTCTGCAG 
TTTGAAGAAG AAATCACAGA CTCTTATCTG CTGATCAGTA GTGCCAGCAG CCCCTTGGAG 
AGCTGGGAGA TTCCTGTATA TCGCTTAAAC ATGTTGGTCC ACTTGCTCAA CAAAGATGTC 
CACACCGTTG TACCGCCTGC GACTAAGACG ACAGTGGAAG AGGTCAGTGA AGAGCAGTAC 
TACATGGTGC GAAGATATGA AAACCAAACT CTTCATCCAC CGCCCAGGCC TCCCAAAAAG 
CCAGCACCAC TGGCACCACA AACTGTTTCT GCACCACCTA AGCAGGGTGT GACTGATGCA 
CTCCAGGCTC CAAAGGTAAC GGATCACGCC TCTTCTTTGA ACCTCTTACA ACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010134115.1
CDS	1..1797
Translation	MASTLEKFIHSLDPRALPRVLQIQSGIYFQGSIYEMFGNECCLSTGEIIKVIGFKINKLIASICENNEVSQLSAKVELPLNFPGLYKVVADKTPYVSIEEITRKISIGPTRFGHPCFYSPEDIKLANLTIKRGEQITFNSVEEVNGTMAVNCGVVRNNQSHSFTLPLSQEGKFYECEDDQIYTLKEIAEWKIPKCRSRIVKLSNILHTWDSSNPLPENFDGCLTLTPVYEVQAVMKFRKDIVHILSDLDVEVKDITDCYDISSFLQPLTLEDIFERTSKEFPMVAEIMEGPSGSPKPYNSLYTGKEIVIYKKYQATRVLASEIRSDSPKRHFLIPMSYKGKFKRRPREFPTAYDLEIARSKEEQLHVVATKAFGSPHEELFSVSVGDQFLVQQCQTSEVLYEGRKKLVDVLACEQILSDAYKKVLVPMYMEGGFVEVVHDKKQYQLSEICKRFRLPFNVKVAVRDLSVEEDVLAAVPGLQFEEEITDSYLLISSASSPLESWEIPVYRLNMLVHLLNKDVHTVVPPATKTTVEEVSEEQYYMVRRYENQTLHPPPRPPKKPAPLAPQTVSAPPKQGVTDALQAPKVTDHASSLNLLQL

Target ORF information:

RefSeq Version	XM_010135813.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris thymocyte selection associated (THEMIS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010135813.1

ORF Insert Sequence:

ATGGCATCTA CGCTGGAGAA GTTCATTCAT TCCCTGGACC CCAGAGCCCT TCCCAGGGTC 
CTCCAAATCC AGTCTGGCAT CTATTTCCAA GGCTCCATCT ATGAAATGTT TGGAAACGAA 
TGCTGCCTGT CAACAGGAGA AATCATTAAA GTTATTGGCT TCAAAATTAA TAAGCTTATA 
GCAAGTATCT GCGAGAATAA TGAAGTCAGC CAGCTTTCAG CCAAAGTGGA ATTGCCACTA 
AATTTTCCTG GTCTTTATAA GGTTGTGGCA GATAAAACTC CGTATGTCAG CATCGAAGAA 
ATTACAAGAA AAATCTCTAT TGGGCCCACA AGATTTGGCC ATCCATGTTT CTACAGCCCC 
GAGGATATAA AACTGGCAAA CCTCACTATC AAACGCGGTG AGCAGATCAC CTTCAACTCA 
GTGGAAGAGG TCAACGGAAC GATGGCTGTG AACTGTGGCG TGGTCAGAAA CAACCAGTCT 
CATTCCTTCA CCCTGCCCCT GTCGCAAGAG GGAAAATTCT ACGAGTGTGA AGATGACCAG 
ATATACACCC TCAAAGAGAT CGCAGAGTGG AAAATCCCCA AGTGCAGGAG CCGGATCGTC 
AAACTTTCCA ATATTTTACA CACGTGGGAC AGCTCTAATC CACTTCCTGA GAATTTTGAC 
GGCTGCTTGA CCCTCACGCC TGTCTATGAA GTGCAGGCAG TGATGAAATT TCGAAAGGAC 
ATAGTTCATA TCCTCTCAGA TCTGGATGTT GAGGTCAAGG ATATAACAGA CTGCTATGAT 
ATTAGCTCTT TCCTTCAGCC ACTGACTTTG GAAGATATTT TTGAAAGAAC AAGCAAGGAA 
TTTCCTATGG TTGCTGAGAT AATGGAAGGG CCTTCGGGAA GCCCAAAACC TTATAACTCA 
CTGTATACAG GAAAAGAGAT CGTCATCTAC AAAAAGTACC AGGCAACAAG AGTCCTAGCC 
TCGGAGATCA GAAGCGATTC CCCCAAAAGA CATTTTTTAA TCCCTATGAG CTACAAAGGA 
AAGTTCAAGA GAAGACCTCG GGAGTTTCCG ACTGCCTACG ACTTAGAGAT AGCAAGAAGC 
AAAGAGGAAC AGCTTCATGT TGTAGCAACT AAAGCCTTTG GTTCCCCTCA TGAAGAGCTT 
TTTTCTGTTT CTGTTGGAGA TCAATTTCTG GTTCAGCAAT GCCAGACCAG TGAAGTTCTG 
TACGAGGGAA GAAAGAAACT CGTAGATGTT TTGGCTTGTG AACAAATACT GAGTGATGCG 
TATAAGAAAG TGCTCGTCCC TATGTACATG GAAGGCGGCT TTGTAGAGGT GGTTCACGAC 
AAGAAACAGT ACCAGCTTTC TGAGATCTGC AAACGCTTTC GTCTGCCTTT TAATGTGAAA 
GTGGCCGTGA GGGACCTTTC CGTTGAGGAG GATGTCTTGG CTGCCGTGCC TGGTCTGCAG 
TTTGAAGAAG AAATCACAGA CTCTTATCTG CTGATCAGTA GTGCCAGCAG CCCCTTGGAG 
AGCTGGGAGA TTCCTGTATA TCGCTTAAAC ATGTTGGTCC ACTTGCTCAA CAAAGATGTC 
CACACCGTTG TACCGCCTGC GACTAAGACG ACAGTGGAAG AGGTCAGTGA AGAGCAGTAC 
TACATGGTGC GAAGATATGA AAACCAAACT CTTCATCCAC CGCCCAGGCC TCCCAAAAAG 
CCAGCACCAC TGGCACCACA AACTGTTTCT GCACCACCTA AGCAGGGTGT GACTGATGCA 
CTCCAGGCTC CAAAGGTAAC GGATCACGCC TCTTCTTTGA ACCTCTTACA ACTGTAG