RNF185 cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF185
Entrez Gene ID	104489426
Full Name	ring finger protein 185
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010131585.1	XP_010129887.1	E3 ubiquitin-protein ligase RNF185 isoform X1
XM_010131586.1	XP_010129888.1	E3 ubiquitin-protein ligase RNF185 isoform X2

Pathways from BioSystems
Gene Ontology

The following RNF185 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF185 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu35696	XM_010131585.1 Latest version!	Buceros rhinoceros silvestris ring finger protein 185 (RNF185), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OBu35697	XM_010131586.1 Latest version!	Buceros rhinoceros silvestris ring finger protein 185 (RNF185), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF185 ( XM_010131585.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010129887.1 Buceros rhinoceros silvestris ring finger protein 185 (RNF185), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu35696
Clone ID Related Accession (Same CDS sequence)	XM_010131585.1
Accession Version	XM_010131585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	E3 ubiquitin-protein ligase RNF185 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010399685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA AAGGACCCAC AACCTCTACT TCAACAAAGA ACTCCAGTAC TGGAGGGACC 
AGTGGCAGCA GTAGCAGTAA TGGTGCTGGG GACAATGCAA ATCAGGACAA CACTTTCGAG 
TGTAACATCT GTTTAGACAC TGCCAAGGAT GCAGTTATCA GCTTGTGTGG ACATCTCTTC 
TGTTGGCCTT GTTTACACCA GTGGCTAGAG ACCAGGCCAA ACAGACAAGT GTGTCCTGTA 
TGCAAAGCAG GAATCAGTCG AGATAAAGTT ATTCCTTTGT ATGGAAGAGG TAGCACTGGG 
CAACAGGACC CCAGAGAGAA GACTCCACCA CGACCCCAAG GACAGAGACC TGAACCAGAA 
AACAGAGGAG GATTCCAGGG CTTTGGGTTT GGGGATGGTG GCTTCCAAAT GTCATTCGGA 
ATTGGGGCGT TTCCCTTTGG TATTTTTGCA ACAGCGTTCA ACATAAACGA CGGGCGACCT 
CCTCCAGCTG TTCCAGGGAC TCCTCAATAT GTGGATGAGC AGTTCCTATC CCGCCTCTTC 
CTGTTTGTGG CTCTGGTGAT AATGTTCTGG CTGTTGATTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010129887.1
CDS	49..633
Translation	MASKGPTTSTSTKNSSTGGTSGSSSSNGAGDNANQDNTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA

Target ORF information:

RefSeq Version	XM_010131585.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris ring finger protein 185 (RNF185), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010131585.1

ORF Insert Sequence:

ATGGCAAGCA AAGGACCCAC AACCTCTACT TCAACAAAGA ACTCCAGTAC TGGAGGGACC 
AGTGGCAGCA GTAGCAGTAA TGGTGCTGGG GACAATGCAA ATCAGGACAA CACTTTCGAG 
TGTAACATCT GTTTAGACAC TGCCAAGGAT GCAGTTATCA GCTTGTGTGG ACATCTCTTC 
TGTTGGCCTT GTTTACACCA GTGGCTAGAG ACCAGGCCAA ACAGACAAGT GTGTCCTGTA 
TGCAAAGCAG GAATCAGTCG AGATAAAGTT ATTCCTTTGT ATGGAAGAGG TAGCACTGGG 
CAACAGGACC CCAGAGAGAA GACTCCACCA CGACCCCAAG GACAGAGACC TGAACCAGAA 
AACAGAGGAG GATTCCAGGG CTTTGGGTTT GGGGATGGTG GCTTCCAAAT GTCATTCGGA 
ATTGGGGCGT TTCCCTTTGG TATTTTTGCA ACAGCGTTCA ACATAAACGA CGGGCGACCT 
CCTCCAGCTG TTCCAGGGAC TCCTCAATAT GTGGATGAGC AGTTCCTATC CCGCCTCTTC 
CTGTTTGTGG CTCTGGTGAT AATGTTCTGG CTGTTGATTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF185 ( XM_010131586.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010129888.1 Buceros rhinoceros silvestris ring finger protein 185 (RNF185), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu35697
Clone ID Related Accession (Same CDS sequence)	XM_010131586.1
Accession Version	XM_010131586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	E3 ubiquitin-protein ligase RNF185 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010399685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA AAGGACCCAC AACCTCTACT TCAACAAAGA ACTCCAGTAC TGGAGGGACC 
AGTGGCAGCA GTAGCAGTAA TGGTGCTGGG GACAATGCAA ATCAGGACAA CACTTTCGAG 
TGTAACATCT GTTTAGACAC TGCCAAGGAT GCAGTTATCA GCTTGTGTGG ACATCTCTTC 
TGTTGGCCTT GTTTACACCA GGGATTCCAG GGCTTTGGGT TTGGGGATGG TGGCTTCCAA 
ATGTCATTCG GAATTGGGGC GTTTCCCTTT GGTATTTTTG CAACAGCGTT CAACATAAAC 
GACGGGCGAC CTCCTCCAGC TGTTCCAGGG ACTCCTCAAT ATGTGGATGA GCAGTTCCTA 
TCCCGCCTCT TCCTGTTTGT GGCTCTGGTG ATAATGTTCT GGCTGTTGAT TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010129888.1
CDS	1..417
Translation	MASKGPTTSTSTKNSSTGGTSGSSSSNGAGDNANQDNTFECNICLDTAKDAVISLCGHLFCWPCLHQGFQGFGFGDGGFQMSFGIGAFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA

Target ORF information:

RefSeq Version	XM_010131586.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris ring finger protein 185 (RNF185), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010131586.1

ORF Insert Sequence:

ATGGCAAGCA AAGGACCCAC AACCTCTACT TCAACAAAGA ACTCCAGTAC TGGAGGGACC 
AGTGGCAGCA GTAGCAGTAA TGGTGCTGGG GACAATGCAA ATCAGGACAA CACTTTCGAG 
TGTAACATCT GTTTAGACAC TGCCAAGGAT GCAGTTATCA GCTTGTGTGG ACATCTCTTC 
TGTTGGCCTT GTTTACACCA GGGATTCCAG GGCTTTGGGT TTGGGGATGG TGGCTTCCAA 
ATGTCATTCG GAATTGGGGC GTTTCCCTTT GGTATTTTTG CAACAGCGTT CAACATAAAC 
GACGGGCGAC CTCCTCCAGC TGTTCCAGGG ACTCCTCAAT ATGTGGATGA GCAGTTCCTA 
TCCCGCCTCT TCCTGTTTGT GGCTCTGGTG ATAATGTTCT GGCTGTTGAT TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.